MD11G1137000.v1.1

Serine threonine-protein phosphatase 6 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008361
Physical Location & Seq
Forward (+)
12636253 .. 12645189
8937 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1137000.v1.1.491

Sequence Viewer

Length: 2550 bp
ATGTTTTGGAAGCTCACTTCTGCCGCCTCTCCTGTGGAGACAGTGCTAGACAAGGAAAATTTTACATTAGAGGAGCTTCTGGATGAAGAAGAGATTATCCAAGAATGCAAAGCCTTAAACACTCGTCTCATTAATTTTTTACGGGATAGAGCCCAGGTGGAGCAATTATTGCGTTATATTGTTGAAGAACCTCCTGAGGATGCTGAAAGCAAACGAGCCTTCAAGTTCCCCTTCATTGCTTGTGAGGTTTTTACTTGTGAAATTGATGTTATCTTAAAGACTTTGGTGGATGAAGAGGAGCTAATGAACTTACTTTTCTCTTTCTTGGAACCAAATCGTCCTCATAGTGCCCCGTTGGCTGGGTATTTTGGCAAGGTTGTTGTATGTCTAATGATACGGAAGACAGCCCCACTTATGAACTATGTTCAAGCCCATCAGGATGTTTTCCGTCAGCTAGTTGATTTGATAGGAATTACGTCCATCATGGAGGTTTTGGTTCGACTTGTTGGTGCGGACGACCATGTGTATCCCAATTTTATTGATGTGATGCAATGGTTAGCTGAAAGTAATCTGCTTGAAATGATCGTAGATAAGTTGAGCCCAACTAATTCTCCTGAAGTTCATGCTAATGTAGCTGAAACACTTTCTGCTATAACTCGAAATGCTCCTTCGGCCCTAGCCAACAAACTTTCTAGCCCAAGTTTTGTTACAAGGATATTTGGTCACGCATTGGAAGATTCACATTCAAAGTCTGGTCTTGTACACTCACTTTCTGTCTGCATCTCTTTGTTGGATCCGAAAAGATCAGCAGTTTCTTCTCCCTTGTTCCATTCTTTTCGGGGCCAACATATGTATGAGTCTCCAGTCCCTGTTAATCCAGATACTGTCAGTGCAATGCTCCCAAAACTTGGCGACTTGCTTGTGCTTTTGAATGTGTCATGGGATGAGAAAACATTGCCGACGACATACGGAGAGTTGAGGCCTCCACTTGGAAAGCATCGTTTAAAGATTGTGGAGTTCATTGCTGTTCTACTGAGATCAGGCAATGAAGAGGCAGAAAAGGAATTGGTTAGCTCAGGAACCATCCAACGAGTCATTGATCTTTTCTTTGAGTACCCTTACAATAATTCCTTGCATCATCATGTAGATAGTATAATATCGTCATGTTTGGAAAGCAAGAGTGATGCCATTGTTGACCATCTTCTTCGAGAGTGTGATTTAATTGGAAAGTTTCTTCAAACGGATAAAAATCCCATTCTATCTGGTGATGCTAATAAGCCAACGGTACCTGCTACTGGAAAATCAGCACCACGAGCAGGAAACCTCGGACACATCACAAGAATTTCTAATAAGCTCATTCAGTTGGGAAACAGCCAAAGCCGGGTTCAGGCATGCCTTCAGGAAAATAGCGACTGGAATGGGTGGCAAACAACAGTTTTGCAGGAGCGCAATGCAGTTGAAAATGTGTATCGATGGGCTTGCGGTCGCCCTACTGCTTTGCAAGACCGGACAAGGGATAGTGACGAGGATGACATGAATGACAGAGATTATGATGTAGCGGCTCTAGCAAATAATCTAAGCCAGGCTTTTAGATACAAAATCTATGGGAATGAGGATGCAGGAGAGGACCAAGGAGCTCTTGATCGAGATGATGAGGATGTCTACTTTGATGATGAATCTGCTGAAGTGGTCATATCATCCTTGCGTCTTGGTGATGACCAAGGAAGTTTATTCACAAATTCCAACTGGTTTGCATTCCAAGATGATAGAACCGGTGATGAACCTGTGGGCACATCACTGTCAGAGATGGAAGAAGTAAACTTGAACGGCACTGCAAATGGTGGTAATAGCAGTAGTGATGATGAGGTTGTGGTAGGGGAGGATGATGAGTTGGCCGGGAGTAAAGAGACTGTCAATGGCGCGTCCAGTTCCAACAGAAACCTTATGAATGGATTTAGCAACGGTGGTAACATGAACCCAGATGGCGAGAAGACAAGTGCCTCCAGTGACTTGGGGGGTTTCTTCAGATTTGAGACATCTGATAATGATGACATGTTTGGAGACAGGCCTTTGCCTGAATGGGTGGGATGGAGTGAATCATCAGATTTGCAAGTCGGCGGCGCAAGTGTTAATCCATTTGAGGATCATGATGACGCAGTTGTCAATCCTTCAACTGCCGAGGTAGTGGCACCAGATGCTGGTGCTAGTTTGCACTCAAGTGGAGAACCTACACTTCCAAATGGCTCTCCTCCATCCTCCACTGCTTCTACTGCAGGGTCAGTGGGCAGTGATGTGAGCCAGAGATCAGCTGCTGTCCCATCACTGTTTGAAGAGGATGTTGAATTCGTGGGTGTGGAATTGGATGGTACTGAGAAGGCTATGGAGCAGGCTCTTAAAGAGGGTATCGTAGGGGAGGCAGGGCCATTGAAGAAAAACATTATGCCGAAGGTGCCAGAAAAAGAGAACCCTGATGACGGTGAGGCTGCAAATAAGGAGTTCAATGACACCAACTATTGGAGGGTTGATCAGGAAGTGGCTGTTTTGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAPS

Protein Analysis

850

Amino Acids

93.25

Weight (kDa)

4.46

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAPS PF04499 129 - 489 1.1e-65 SIT4 phosphatase-associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2159
Acc36I ACCTGC 1 cut(s) 1297
Acc65I GGTACC 1 cut(s) 1285
AccB1I GGYRCC 3 cut(s) 1285, 2188, 2449
AccB7I CCANNNNNTGG 3 cut(s) 908, 2198, 2514
AccI GTMKAC 1 cut(s) 1662
AccII CGCG 1 cut(s) 1922
AciI CCGC 5 cut(s) 24, 512, 1482, 1559, 2118
AclWI GGATC 3 cut(s) 788, 801, 2151
AcoI YGGCCR 1 cut(s) 1893
AcsI RAATTY 4 cut(s) 58, 1341, 1738, 2342
AcuI CTGAAG 4 cut(s) 636, 1382, 1704, 2008
AfaI GTAC 4 cut(s) 762, 1115, 1287, 2368
AflIII ACRYGT 1 cut(s) 2052
AgeI ACCGGT 1 cut(s) 1772
AjnI CCWGG 2 cut(s) 153, 1581
AjuI GAANNNNNNNTTGG 2 cut(s) 1368, 1400
AleI CACNNNNGTG 1 cut(s) 2217
Alw21I GWGCWC 1 cut(s) 1639
Alw26I GTCTC 6 cut(s) 32, 131, 864, 1901, 2027, 2055
AlwI GGATC 3 cut(s) 788, 801, 2151
AlwNI CAGNNNCTG 4 cut(s) 869, 884, 2198, 2312
AoxI GGCC 6 cut(s) 672, 841, 980, 1893, 2066, 2420
ApeKI GCWGC 2 cut(s) 2309, 2483
ApoI RAATTY 4 cut(s) 58, 1341, 1738, 2342
AseI ATTAAT 1 cut(s) 132
AsiGI ACCGGT 1 cut(s) 1772
Asp718I GGTACC 1 cut(s) 1285
AspLEI GCGC 3 cut(s) 1449, 1922, 2123
AspS9I GGNCC 4 cut(s) 673, 841, 1627, 2420
AsuC2I CCSGG 2 cut(s) 1382, 1897
AsuHPI GGTGA 4 cut(s) 1277, 1724, 1787, 2489
AvaII GGWCC 1 cut(s) 1627
AxyI CCTNAGG 1 cut(s) 195
BaeGI GKGCMC 2 cut(s) 352, 1793
BamHI GGATCC 1 cut(s) 793
BanI GGYRCC 3 cut(s) 1285, 2188, 2449
BanII GRGCYC 3 cut(s) 154, 602, 1639
BauI CACGAG 1 cut(s) 1311
BbsI GAAGAC 2 cut(s) 407, 1997
Bbv12I GWGCWC 1 cut(s) 1639
BbvI GCAGC 2 cut(s) 2296, 2470
BceAI ACGGC 1 cut(s) 1843
BcgI CGANNNNNNTGC 2 cut(s) 1461, 1495
BciT130I CCWGG 2 cut(s) 155, 1583
BciVI GTATCC 1 cut(s) 537
BclI TGATCA 1 cut(s) 2524
BcnI CCSGG 2 cut(s) 1382, 1897
BcoDI GTCTC 6 cut(s) 32, 131, 864, 1901, 2027, 2055
BfaI CTAG 6 cut(s) 47, 455, 677, 693, 1565, 2205
BfmI CTRYAG 1 cut(s) 2271
BfuAI ACCTGC 1 cut(s) 1297
BfuI GTATCC 1 cut(s) 537
BglI GCCNNNNNGGC 1 cut(s) 356
BisI GCNGC 5 cut(s) 24, 1560, 2119, 2310, 2484
BlsI GCNGC 5 cut(s) 25, 1561, 2120, 2311, 2485
Bme1390I CCNGG 4 cut(s) 155, 1382, 1583, 1897
Bme18I GGWCC 1 cut(s) 1627
BmgT120I GGNCC 4 cut(s) 673, 841, 1627, 2420
BmiI GGNNCC 7 cut(s) 330, 795, 842, 1081, 1287, 2190, 2451
BmrFI CCNGG 4 cut(s) 155, 1382, 1583, 1897
BmsI GCATC 9 cut(s) 190, 537, 789, 1006, 1144, 1174, 1258, 1606, 2185
BpiI GAAGAC 2 cut(s) 407, 1997
BpmI CTGGAG 2 cut(s) 846, 1987
Bpu10I CCTNAGC 1 cut(s) 1075
BpuEI CTTGAG 1 cut(s) 2200
BpuMI CCSGG 2 cut(s) 1382, 1897
Bsa29I ATCGAT 1 cut(s) 1471
BsaBI GATNNNNATC 1 cut(s) 1248
BsaJI CCNNGG 5 cut(s) 153, 1324, 1630, 1720, 2178
BsaWI WCCGGW 2 cut(s) 1506, 1772
BsaXI ACNNNNNCTCC 2 cut(s) 595, 625
Bse118I RCCGGY 1 cut(s) 1772
Bse1I ACTGG 6 cut(s) 863, 1300, 1418, 1751, 1926, 2004
Bse21I CCTNAGG 1 cut(s) 195
Bse3DI GCAATG 7 cut(s) 234, 557, 900, 953, 1020, 1051, 1456
Bse8I GATNNNNATC 1 cut(s) 1248
BseBI CCWGG 2 cut(s) 155, 1583
BseCI ATCGAT 1 cut(s) 1471
BseDI CCNNGG 5 cut(s) 153, 1324, 1630, 1720, 2178
BseJI GATNNNNATC 1 cut(s) 1248
BseMI GCAATG 7 cut(s) 234, 557, 900, 953, 1020, 1051, 1456
BseMII CTCAG 4 cut(s) 186, 1025, 1089, 2361
BseNI ACTGG 6 cut(s) 863, 1300, 1418, 1751, 1926, 2004
BseRI GAGGAG 3 cut(s) 86, 311, 2238
BseSI GKGCMC 2 cut(s) 352, 1793
BseXI GCAGC 2 cut(s) 2296, 2470
BseYI CCCAGC 1 cut(s) 359
Bsh1236I CGCG 1 cut(s) 1922
Bsh1285I CGRYCG 1 cut(s) 1486
BshFI GGCC 6 cut(s) 674, 843, 982, 1895, 2068, 2422
BshNI GGYRCC 3 cut(s) 1285, 2188, 2449
BshTI ACCGGT 1 cut(s) 1772
BshVI ATCGAT 1 cut(s) 1471
BsiEI CGRYCG 1 cut(s) 1486
BsiHKAI GWGCWC 1 cut(s) 1639
BsiSI CCGG 4 cut(s) 1381, 1507, 1773, 1896
BslFI GGGAC 2 cut(s) 851, 2300
BsmAI GTCTC 6 cut(s) 32, 131, 864, 1901, 2027, 2055
BsmBI CGTCTC 1 cut(s) 131
BsmFI GGGAC 2 cut(s) 851, 2300
BsmI GAATGC 2 cut(s) 110, 1754
BsnI GGCC 6 cut(s) 674, 843, 982, 1895, 2068, 2422
Bsp1286I GDGCHC 5 cut(s) 154, 352, 602, 1639, 1793
Bsp1407I TGTACA 1 cut(s) 760
Bsp143I GATC 9 cut(s) 582, 793, 803, 1037, 1099, 1642, 2143, 2303, 2524
BspACI CCGC 5 cut(s) 24, 512, 1482, 1559, 2118
BspANI GGCC 6 cut(s) 674, 843, 982, 1895, 2068, 2422
BspCNI CTCAG 4 cut(s) 187, 1026, 1088, 2362
BspDI ATCGAT 1 cut(s) 1471
BspFNI CGCG 1 cut(s) 1922
BspHI TCATGA 1 cut(s) 2146
BspLI GGNNCC 7 cut(s) 330, 795, 842, 1081, 1287, 2190, 2451
BspMAI CTGCAG 1 cut(s) 2275
BspMI ACCTGC 1 cut(s) 1297
BspPI GGATC 3 cut(s) 788, 801, 2151
BspT107I GGYRCC 3 cut(s) 1285, 2188, 2449
BsrDI GCAATG 7 cut(s) 234, 557, 900, 953, 1020, 1051, 1456
BsrFI RCCGGY 1 cut(s) 1772
BsrGI TGTACA 1 cut(s) 760
BsrI ACTGG 6 cut(s) 863, 1300, 1418, 1751, 1926, 2004
BssAI RCCGGY 1 cut(s) 1772
BssECI CCNNGG 5 cut(s) 153, 1324, 1630, 1720, 2178
BssMI GATC 9 cut(s) 582, 793, 803, 1037, 1099, 1642, 2143, 2303, 2524
BssSI CACGAG 1 cut(s) 1311
BssT1I CCWWGG 2 cut(s) 1630, 1720
Bst2BI CACGAG 1 cut(s) 1311
Bst2UI CCWGG 2 cut(s) 155, 1583
Bst4CI ACNGT 9 cut(s) 43, 886, 1285, 1435, 1800, 1912, 1964, 2325, 2477
Bst6I CTCTTC 4 cut(s) 84, 288, 1044, 2325
BstAPI GCANNNNNTGC 2 cut(s) 169, 2195
BstAUI TGTACA 1 cut(s) 760
BstC8I GCNNGC 3 cut(s) 1393, 1480, 2388
BstDEI CTNAG 5 cut(s) 195, 1034, 1075, 1577, 2370
BstFNI CGCG 1 cut(s) 1922
BstHHI GCGC 3 cut(s) 1449, 1922, 2123
BstKTI GATC 9 cut(s) 585, 796, 806, 1040, 1102, 1645, 2146, 2306, 2527
BstMAI GTCTC 6 cut(s) 32, 131, 864, 1901, 2027, 2055
BstMBI GATC 9 cut(s) 582, 793, 803, 1037, 1099, 1642, 2143, 2303, 2524
BstMCI CGRYCG 1 cut(s) 1486
BstMWI GCNNNNNNNGC 9 cut(s) 169, 356, 632, 671, 1313, 1565, 2195, 2270, 2449
BstNI CCWGG 2 cut(s) 155, 1583
BstNSI RCATGY 2 cut(s) 1395, 2056
BstSCI CCNGG 4 cut(s) 153, 1380, 1581, 1895
BstSFI CTRYAG 1 cut(s) 2271
BstSLI GKGCMC 2 cut(s) 352, 1793
BstUI CGCG 1 cut(s) 1922
BstV1I GCAGC 2 cut(s) 2296, 2470
BstV2I GAAGAC 2 cut(s) 407, 1997
BstX2I RGATCY 1 cut(s) 793
BstXI CCANNNNNNTGG 1 cut(s) 2011
BstYI RGATCY 1 cut(s) 793
Bsu15I ATCGAT 1 cut(s) 1471
Bsu36I CCTNAGG 1 cut(s) 195
BsuI GTATCC 1 cut(s) 537
BsuRI GGCC 6 cut(s) 674, 843, 982, 1895, 2068, 2422
BsuTUI ATCGAT 1 cut(s) 1471
BtsI GCAGTG 3 cut(s) 1830, 2259, 2293
BtsIMutI CAGTG 9 cut(s) 48, 895, 1796, 1830, 2011, 2259, 2286, 2293, 2321
BveI ACCTGC 1 cut(s) 1297
Cac8I GCNNGC 3 cut(s) 1393, 1480, 2388
CaiI CAGNNNCTG 4 cut(s) 869, 884, 2198, 2312
CciI TCATGA 1 cut(s) 2146
CfoI GCGC 3 cut(s) 1449, 1922, 2123
Cfr10I RCCGGY 1 cut(s) 1772
Cfr13I GGNCC 4 cut(s) 673, 841, 1627, 2420
ClaI ATCGAT 1 cut(s) 1471
CseI GACGC 3 cut(s) 1694, 1911, 2162
Csp6I GTAC 4 cut(s) 761, 1114, 1286, 2367
CspAI ACCGGT 1 cut(s) 1772
CviQI GTAC 4 cut(s) 761, 1114, 1286, 2367
DdeI CTNAG 5 cut(s) 195, 1034, 1075, 1577, 2370
DpnI GATC 9 cut(s) 584, 795, 805, 1039, 1101, 1644, 2145, 2305, 2526
DpnII GATC 9 cut(s) 582, 793, 803, 1037, 1099, 1642, 2143, 2303, 2524
DraI TTTAAA 1 cut(s) 1005
DrdI GACNNNNNNGTC 1 cut(s) 2159
DseDI GACNNNNNNGTC 1 cut(s) 2159
EaeI YGGCCR 1 cut(s) 1893
Eam1104I CTCTTC 4 cut(s) 84, 288, 1044, 2325
EarI CTCTTC 4 cut(s) 84, 288, 1044, 2325
Ecl136II GAGCTC 1 cut(s) 1637
Eco130I CCWWGG 2 cut(s) 1630, 1720
Eco147I AGGCCT 2 cut(s) 982, 2068
Eco24I GRGCYC 3 cut(s) 154, 602, 1639
Eco47I GGWCC 1 cut(s) 1627
Eco53kI GAGCTC 1 cut(s) 1637
Eco57I CTGAAG 4 cut(s) 636, 1382, 1704, 2008
Eco81I CCTNAGG 1 cut(s) 195
EcoICRI GAGCTC 1 cut(s) 1637
EcoRI GAATTC 1 cut(s) 2342
EcoRII CCWGG 2 cut(s) 153, 1581
EcoT14I CCWWGG 2 cut(s) 1630, 1720
EcoT38I GRGCYC 3 cut(s) 154, 602, 1639
ErhI CCWWGG 2 cut(s) 1630, 1720
Esp3I CGTCTC 1 cut(s) 131
FalI AAGNNNNNCTT 6 cut(s) 215, 247, 1570, 1602, 1623, 1655
FaqI GGGAC 2 cut(s) 851, 2300
FauNDI CATATG 1 cut(s) 849
FbaI TGATCA 1 cut(s) 2524
FblI GTMKAC 1 cut(s) 1662
Fnu4HI GCNGC 5 cut(s) 24, 1560, 2119, 2310, 2484
FriOI GRGCYC 3 cut(s) 154, 602, 1639
Fsp4HI GCNGC 5 cut(s) 24, 1560, 2119, 2310, 2484
FspBI CTAG 6 cut(s) 47, 455, 677, 693, 1565, 2205
GlaI GCGC 3 cut(s) 1448, 1921, 2122
GluI GCNGC 5 cut(s) 24, 1560, 2119, 2310, 2484
GsaI CCCAGC 1 cut(s) 363
GsuI CTGGAG 2 cut(s) 846, 1987
HaeIII GGCC 6 cut(s) 674, 843, 982, 1895, 2068, 2422
HapII CCGG 4 cut(s) 1381, 1507, 1773, 1896
HgaI GACGC 3 cut(s) 1694, 1911, 2162
HhaI GCGC 3 cut(s) 1449, 1922, 2123
Hin6I GCGC 3 cut(s) 1447, 1920, 2121
HinP1I GCGC 3 cut(s) 1447, 1920, 2121
HincII GTYRAC 1 cut(s) 1195
HindII GTYRAC 1 cut(s) 1195
HinfI GANTC 5 cut(s) 737, 857, 1092, 1676, 2096
HpaII CCGG 4 cut(s) 1381, 1507, 1773, 1896
HphI GGTGA 4 cut(s) 1277, 1724, 1787, 2489
Hpy166II GTNNAC 4 cut(s) 763, 1195, 1663, 1819
Hpy188I TCNGA 6 cut(s) 798, 1328, 1804, 2027, 2041, 2104
Hpy8I GTNNAC 4 cut(s) 763, 1195, 1663, 1819
Hpy99I CGWCG 1 cut(s) 964
HpyAV CCTTC 7 cut(s) 229, 241, 678, 1406, 2178, 2368, 2440
HpyCH4III ACNGT 9 cut(s) 43, 886, 1285, 1435, 1800, 1912, 1964, 2325, 2477
HpyCH4IV ACGT 1 cut(s) 476
HpyF10VI GCNNNNNNNGC 9 cut(s) 169, 356, 632, 671, 1313, 1565, 2195, 2270, 2449
HpyF3I CTNAG 5 cut(s) 195, 1034, 1075, 1577, 2370
HpySE526I ACGT 1 cut(s) 476
HspAI GCGC 3 cut(s) 1447, 1920, 2121
KpnI GGTACC 1 cut(s) 1289
Ksp22I TGATCA 1 cut(s) 2524
Kzo9I GATC 9 cut(s) 582, 793, 803, 1037, 1099, 1642, 2143, 2303, 2524
LmnI GCTCC 8 cut(s) 73, 160, 298, 670, 903, 1444, 1634, 2383
Lsp1109I GCAGC 2 cut(s) 2296, 2470
LweI GCATC 9 cut(s) 190, 537, 789, 1006, 1144, 1174, 1258, 1606, 2185
MaeI CTAG 6 cut(s) 47, 455, 677, 693, 1565, 2205
MaeII ACGT 1 cut(s) 476
MaeIII GTNAC 5 cut(s) 706, 722, 1520, 1967, 2006
MalI GATC 9 cut(s) 584, 795, 805, 1039, 1101, 1644, 2145, 2305, 2526
MboI GATC 9 cut(s) 582, 793, 803, 1037, 1099, 1642, 2143, 2303, 2524
MflI RGATCY 1 cut(s) 793
MhlI GDGCHC 5 cut(s) 154, 352, 602, 1639, 1793
MlyI GAGTC 2 cut(s) 866, 1101
MmeI TCCRAC 4 cut(s) 771, 1111, 1767, 1956
MseI TTAA 8 cut(s) 116, 132, 275, 873, 1004, 1220, 2130, 2394
MslI CAYNNNNRTG 5 cut(s) 438, 627, 852, 1140, 2217
MspA1I CMGCKG 1 cut(s) 2309
MspI CCGG 4 cut(s) 1381, 1507, 1773, 1896
MspR9I CCNGG 4 cut(s) 155, 1382, 1583, 1897
Mva1269I GAATGC 2 cut(s) 110, 1754
MvaI CCWGG 2 cut(s) 155, 1583
MvnI CGCG 1 cut(s) 1922
MwoI GCNNNNNNNGC 9 cut(s) 169, 356, 632, 671, 1313, 1565, 2195, 2270, 2449
NciI CCSGG 2 cut(s) 1382, 1897
NdeI CATATG 1 cut(s) 849
NdeII GATC 9 cut(s) 582, 793, 803, 1037, 1099, 1642, 2143, 2303, 2524
NlaIV GGNNCC 7 cut(s) 330, 795, 842, 1081, 1287, 2190, 2451
NmeAIII GCCGAG 1 cut(s) 2203
NmuCI GTSAC 3 cut(s) 722, 1520, 2006
NspI RCATGY 2 cut(s) 1395, 2056
OliI CACNNNNGTG 1 cut(s) 2217
PaeI GCATGC 1 cut(s) 1395
PagI TCATGA 1 cut(s) 2146
PceI AGGCCT 2 cut(s) 982, 2068
PciI ACATGT 1 cut(s) 2052
PctI GAATGC 2 cut(s) 110, 1754
PfeI GAWTC 3 cut(s) 737, 1676, 2096
PflMI CCANNNNNTGG 3 cut(s) 908, 2198, 2514
PinAI ACCGGT 1 cut(s) 1772
PkrI GCNGC 5 cut(s) 25, 1561, 2120, 2311, 2485
PleI GAGTC 2 cut(s) 865, 1100
PpsI GAGTC 2 cut(s) 865, 1100
PscI ACATGT 1 cut(s) 2052
PshBI ATTAAT 1 cut(s) 132
Psp124BI GAGCTC 1 cut(s) 1639
Psp6I CCWGG 2 cut(s) 153, 1581
PspFI CCCAGC 1 cut(s) 359
PspGI CCWGG 2 cut(s) 153, 1581
PspN4I GGNNCC 7 cut(s) 330, 795, 842, 1081, 1287, 2190, 2451
PspPI GGNCC 4 cut(s) 673, 841, 1627, 2420
PstI CTGCAG 1 cut(s) 2275
PstNI CAGNNNCTG 4 cut(s) 869, 884, 2198, 2312
PsuI RGATCY 1 cut(s) 793
PvuII CAGCTG 1 cut(s) 2309
RsaI GTAC 4 cut(s) 762, 1115, 1287, 2368
RsaNI GTAC 4 cut(s) 761, 1114, 1286, 2367
RseI CAYNNNNRTG 5 cut(s) 438, 627, 852, 1140, 2217
SacI GAGCTC 1 cut(s) 1639
SaqAI TTAA 8 cut(s) 116, 132, 275, 873, 1004, 1220, 2130, 2394
SatI GCNGC 5 cut(s) 24, 1560, 2119, 2310, 2484
Sau3AI GATC 9 cut(s) 582, 793, 803, 1037, 1099, 1642, 2143, 2303, 2524
Sau96I GGNCC 4 cut(s) 673, 841, 1627, 2420
SchI GAGTC 2 cut(s) 866, 1101
ScrFI CCNGG 4 cut(s) 155, 1382, 1583, 1897
SduI GDGCHC 5 cut(s) 154, 352, 602, 1639, 1793
SfaNI GCATC 9 cut(s) 190, 537, 789, 1006, 1144, 1174, 1258, 1606, 2185
SfcI CTRYAG 1 cut(s) 2271
SinI GGWCC 1 cut(s) 1627
SmiMI CAYNNNNRTG 5 cut(s) 438, 627, 852, 1140, 2217
SmlI CTYRAG 1 cut(s) 2215
SmoI CTYRAG 1 cut(s) 2215
SphI GCATGC 1 cut(s) 1395
SseBI AGGCCT 2 cut(s) 982, 2068
SsiI CCGC 5 cut(s) 24, 512, 1482, 1559, 2118
SspMI CTAG 6 cut(s) 47, 455, 677, 693, 1565, 2205
SstI GAGCTC 1 cut(s) 1639
StuI AGGCCT 2 cut(s) 982, 2068
StyD4I CCNGG 4 cut(s) 153, 1380, 1581, 1895
StyI CCWWGG 2 cut(s) 1630, 1720
TaaI ACNGT 9 cut(s) 43, 886, 1285, 1435, 1800, 1912, 1964, 2325, 2477
TaiI ACGT 1 cut(s) 479
TaqI TCGA 5 cut(s) 499, 658, 1207, 1471, 1645
TatI WGTACW 1 cut(s) 760
TauI GCSGC 3 cut(s) 26, 1562, 2121
TfiI GAWTC 3 cut(s) 737, 1676, 2096
Tru1I TTAA 8 cut(s) 116, 132, 275, 873, 1004, 1220, 2130, 2394
Tru9I TTAA 8 cut(s) 116, 132, 275, 873, 1004, 1220, 2130, 2394
TscAI CASTG 9 cut(s) 48, 895, 1803, 1837, 2011, 2266, 2286, 2293, 2328
TseFI GTSAC 3 cut(s) 722, 1520, 2006
TseI GCWGC 2 cut(s) 2309, 2483
Tsp45I GTSAC 3 cut(s) 722, 1520, 2006
TspGWI ACGGA 4 cut(s) 412, 437, 984, 1256
TspRI CASTG 9 cut(s) 48, 895, 1803, 1837, 2011, 2266, 2286, 2293, 2328
Van91I CCANNNNNTGG 3 cut(s) 908, 2198, 2514
VpaK11BI GGWCC 1 cut(s) 1627
VspI ATTAAT 1 cut(s) 132
XapI RAATTY 4 cut(s) 58, 1341, 1738, 2342
XceI RCATGY 2 cut(s) 1395, 2056
XmiI GTMKAC 1 cut(s) 1662
XspI CTAG 6 cut(s) 47, 455, 677, 693, 1565, 2205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.