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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G60700 AT5G60700 |
| fragaria_vesca | FvH4_3g31890 FvH4_3g31890 |
| malus_domestica | MD03G1120400.v1.1 MD11G1138800.v1.1 |
| prunus_persica | Prupe.6G103800_v2.0.a1 |
| pyrus_communis | pycom11g11450 |
| rosa_chinensis | RchiOBHm_Chr5g0058661 |
| rosa_laevigata | RLG00000035238 |
| rosa_multiflora | Rmu_sc0000367.1_g000205 |
| rosa_roxburghii | Rroxscaffold_1G00021760 |
| rosa_rugosa | Rorug05G0317100 |
| rosa_samantha | Rh5AG382800 Rh5BG394700 Rh5CG418500 Rh5DG410100 |
| rosa_wichuraiana | Rw5G036070 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 991 |
| Acc36I | ACCTGC | 1 cut(s) | 991 |
| Acc65I | GGTACC | 2 cut(s) | 1885, 2028 |
| AccB1I | GGYRCC | 4 cut(s) | 1885, 2028, 2575, 2641 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1023, 2313 |
| AccI | GTMKAC | 2 cut(s) | 1394, 2331 |
| AccII | CGCG | 1 cut(s) | 860 |
| AciI | CCGC | 4 cut(s) | 300, 836, 860, 1443 |
| AclWI | GGATC | 3 cut(s) | 535, 1144, 2111 |
| AcsI | RAATTY | 5 cut(s) | 19, 1385, 1605, 2321, 2792 |
| AcyI | GRCGYC | 1 cut(s) | 2358 |
| AdeI | CACNNNGTG | 2 cut(s) | 572, 1296 |
| AfaI | GTAC | 9 cut(s) | 106, 728, 776, 851, 1124, 1887, 2030, 2444, 2456 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 622, 1023, 1621, 1953, 2188, 2286, 2313, 2391, 2485 |
| AflIII | ACRYGT | 1 cut(s) | 829 |
| AhlI | ACTAGT | 1 cut(s) | 1936 |
| AjnI | CCWGG | 4 cut(s) | 799, 2390, 2484, 2611 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 261, 293, 848, 880, 1809, 1841 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1128, 1160 |
| Alw21I | GWGCWC | 5 cut(s) | 357, 577, 654, 1534, 2160 |
| Alw26I | GTCTC | 6 cut(s) | 143, 1429, 1676, 2086, 2468, 2677 |
| AlwI | GGATC | 3 cut(s) | 535, 1144, 2111 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1463, 1637 |
| Ama87I | CYCGRG | 2 cut(s) | 1426, 2516 |
| AoxI | GGCC | 7 cut(s) | 514, 1475, 1721, 1955, 1963, 2210, 2683 |
| ApeKI | GCWGC | 6 cut(s) | 814, 870, 1301, 1457, 1843, 2693 |
| ApoI | RAATTY | 5 cut(s) | 19, 1385, 1605, 2321, 2792 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2033, 2065 |
| AseI | ATTAAT | 1 cut(s) | 1644 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 324, 2423, 2657 |
| Asp718I | GGTACC | 2 cut(s) | 1885, 2028 |
| AspS9I | GGNCC | 2 cut(s) | 515, 1964 |
| AsuC2I | CCSGG | 3 cut(s) | 2517, 2518, 2698 |
| AsuHPI | GGTGA | 8 cut(s) | 1066, 1193, 1636, 1804, 1889, 2212, 2341, 2560 |
| AsuII | TTCGAA | 2 cut(s) | 23, 1733 |
| AsuNHI | GCTAGC | 1 cut(s) | 1583 |
| AvaI | CYCGRG | 2 cut(s) | 1426, 2516 |
| BaeGI | GKGCMC | 1 cut(s) | 2524 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 2446, 2479 |
| BanI | GGYRCC | 4 cut(s) | 1885, 2028, 2575, 2641 |
| BanII | GRGCYC | 5 cut(s) | 191, 644, 654, 1534, 2160 |
| BbsI | GAAGAC | 2 cut(s) | 2290, 2598 |
| Bbv12I | GWGCWC | 5 cut(s) | 357, 577, 654, 1534, 2160 |
| BbvCI | CCTCAGC | 1 cut(s) | 1103 |
| BbvI | GCAGC | 6 cut(s) | 801, 882, 1288, 1469, 1830, 2705 |
| BccI | CCATC | 9 cut(s) | 4, 464, 1741, 2256, 2277, 2397, 2653, 2746, 2785 |
| BceAI | ACGGC | 1 cut(s) | 874 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 439, 473, 2478, 2512 |
| BciT130I | CCWGG | 4 cut(s) | 801, 2392, 2486, 2613 |
| BciVI | GTATCC | 1 cut(s) | 1320 |
| BclI | TGATCA | 5 cut(s) | 402, 1255, 1599, 1626, 2758 |
| BcnI | CCSGG | 3 cut(s) | 2517, 2518, 2698 |
| BcoDI | GTCTC | 6 cut(s) | 143, 1429, 1676, 2086, 2468, 2677 |
| BcuI | ACTAGT | 1 cut(s) | 1936 |
| BfaI | CTAG | 3 cut(s) | 114, 1584, 1937 |
| BfmI | CTRYAG | 4 cut(s) | 815, 922, 1035, 2281 |
| BfuAI | ACCTGC | 1 cut(s) | 991 |
| BfuI | GTATCC | 1 cut(s) | 1320 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 895 |
| BglII | AGATCT | 1 cut(s) | 1093 |
| BisI | GCNGC | 6 cut(s) | 815, 871, 1302, 1458, 1844, 2694 |
| BlpI | GCTNAGC | 1 cut(s) | 576 |
| BlsI | GCNGC | 6 cut(s) | 816, 872, 1303, 1459, 1845, 2695 |
| BmcAI | AGTACT | 2 cut(s) | 106, 728 |
| Bme1390I | CCNGG | 7 cut(s) | 801, 2392, 2486, 2517, 2518, 2613, 2698 |
| BmeT110I | CYCGRG | 2 cut(s) | 1426, 2516 |
| BmgT120I | GGNCC | 2 cut(s) | 515, 1964 |
| BmrFI | CCNGG | 7 cut(s) | 801, 2392, 2486, 2517, 2518, 2613, 2698 |
| BmrI | ACTGGG | 1 cut(s) | 2366 |
| BmsI | GCATC | 6 cut(s) | 382, 1315, 1444, 1852, 2642, 2717 |
| BmtI | GCTAGC | 1 cut(s) | 1587 |
| BmuI | ACTGGG | 1 cut(s) | 2366 |
| BpiI | GAAGAC | 2 cut(s) | 2290, 2598 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2147, 2179 |
| BpmI | CTGGAG | 1 cut(s) | 2613 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1103, 2184 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 576 |
| Bpu14I | TTCGAA | 2 cut(s) | 23, 1733 |
| BpuEI | CTTGAG | 4 cut(s) | 395, 1400, 1454, 2084 |
| BpuMI | CCSGG | 3 cut(s) | 2517, 2518, 2698 |
| BsaHI | GRCGYC | 1 cut(s) | 2358 |
| BsaJI | CCNNGG | 8 cut(s) | 800, 1718, 1993, 2352, 2516, 2517, 2535, 2697 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 66, 96 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 622, 1023, 1621, 1953, 2188, 2286, 2313, 2391, 2485 |
| Bse118I | RCCGGY | 1 cut(s) | 1085 |
| Bse1I | ACTGG | 5 cut(s) | 1151, 1285, 1958, 2372, 2669 |
| Bse3DI | GCAATG | 5 cut(s) | 254, 495, 1311, 1769, 2318 |
| BseBI | CCWGG | 4 cut(s) | 801, 2392, 2486, 2613 |
| BseDI | CCNNGG | 8 cut(s) | 800, 1718, 1993, 2352, 2516, 2517, 2535, 2697 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 622, 1023, 1621, 1953, 2188, 2286, 2313, 2391, 2485 |
| BseMI | GCAATG | 5 cut(s) | 254, 495, 1311, 1769, 2318 |
| BseMII | CTCAG | 8 cut(s) | 410, 491, 590, 1117, 1307, 1870, 2220, 2461 |
| BseNI | ACTGG | 5 cut(s) | 1151, 1285, 1958, 2372, 2669 |
| BseRI | GAGGAG | 1 cut(s) | 2152 |
| BseSI | GKGCMC | 1 cut(s) | 2524 |
| BseXI | GCAGC | 6 cut(s) | 801, 882, 1288, 1469, 1830, 2705 |
| BsgI | GTGCAG | 4 cut(s) | 57, 1019, 1082, 2313 |
| Bsh1236I | CGCG | 1 cut(s) | 860 |
| Bsh1285I | CGRYCG | 2 cut(s) | 198, 1086 |
| BshFI | GGCC | 7 cut(s) | 516, 1477, 1723, 1957, 1965, 2212, 2685 |
| BshNI | GGYRCC | 4 cut(s) | 1885, 2028, 2575, 2641 |
| BsiEI | CGRYCG | 2 cut(s) | 198, 1086 |
| BsiHKAI | GWGCWC | 5 cut(s) | 357, 577, 654, 1534, 2160 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1426, 2516 |
| BsiSI | CCGG | 3 cut(s) | 1086, 2517, 2697 |
| BslFI | GGGAC | 2 cut(s) | 853, 2131 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 622, 1023, 1621, 1953, 2188, 2286, 2313, 2391, 2485 |
| BsmAI | GTCTC | 6 cut(s) | 143, 1429, 1676, 2086, 2468, 2677 |
| BsmFI | GGGAC | 2 cut(s) | 853, 2131 |
| BsmI | GAATGC | 3 cut(s) | 60, 1699, 1794 |
| BsnI | GGCC | 7 cut(s) | 516, 1477, 1723, 1957, 1965, 2212, 2685 |
| BsoBI | CYCGRG | 2 cut(s) | 1426, 2516 |
| Bsp119I | TTCGAA | 2 cut(s) | 23, 1733 |
| Bsp1286I | GDGCHC | 8 cut(s) | 191, 357, 577, 644, 654, 1534, 2160, 2524 |
| Bsp1407I | TGTACA | 1 cut(s) | 774 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 576 |
| BspACI | CCGC | 4 cut(s) | 300, 836, 860, 1443 |
| BspANI | GGCC | 7 cut(s) | 516, 1477, 1723, 1957, 1965, 2212, 2685 |
| BspCNI | CTCAG | 8 cut(s) | 409, 490, 589, 1116, 1306, 1869, 2221, 2460 |
| BspFNI | CGCG | 1 cut(s) | 860 |
| BspMAI | CTGCAG | 3 cut(s) | 819, 1039, 2285 |
| BspMI | ACCTGC | 1 cut(s) | 991 |
| BspOI | GCTAGC | 1 cut(s) | 1587 |
| BspPI | GGATC | 3 cut(s) | 535, 1144, 2111 |
| BspT104I | TTCGAA | 2 cut(s) | 23, 1733 |
| BspT107I | GGYRCC | 4 cut(s) | 1885, 2028, 2575, 2641 |
| BsrDI | GCAATG | 5 cut(s) | 254, 495, 1311, 1769, 2318 |
| BsrFI | RCCGGY | 1 cut(s) | 1085 |
| BsrGI | TGTACA | 1 cut(s) | 774 |
| BsrI | ACTGG | 5 cut(s) | 1151, 1285, 1958, 2372, 2669 |
| BssAI | RCCGGY | 1 cut(s) | 1085 |
| BssECI | CCNNGG | 8 cut(s) | 800, 1718, 1993, 2352, 2516, 2517, 2535, 2697 |
| BssNI | GRCGYC | 1 cut(s) | 2358 |
| BssT1I | CCWWGG | 3 cut(s) | 1718, 1993, 2535 |
| Bst2UI | CCWGG | 4 cut(s) | 801, 2392, 2486, 2613 |
| Bst4CI | ACNGT | 6 cut(s) | 538, 1187, 1222, 1399, 1633, 1910 |
| Bst6I | CTCTTC | 2 cut(s) | 1337, 2663 |
| BstACI | GRCGYC | 1 cut(s) | 2358 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 161, 1440, 1463 |
| BstAUI | TGTACA | 1 cut(s) | 774 |
| BstBI | TTCGAA | 2 cut(s) | 23, 1733 |
| BstC8I | GCNNGC | 3 cut(s) | 1585, 2409, 2491 |
| BstFNI | CGCG | 1 cut(s) | 860 |
| BstMAI | GTCTC | 6 cut(s) | 143, 1429, 1676, 2086, 2468, 2677 |
| BstMCI | CGRYCG | 2 cut(s) | 198, 1086 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 161, 895, 958, 1418, 1440, 1463, 1529, 2120, 2699 |
| BstNI | CCWGG | 4 cut(s) | 801, 2392, 2486, 2613 |
| BstNSI | RCATGY | 1 cut(s) | 833 |
| BstSCI | CCNGG | 7 cut(s) | 799, 2390, 2484, 2515, 2516, 2611, 2696 |
| BstSFI | CTRYAG | 4 cut(s) | 815, 922, 1035, 2281 |
| BstSLI | GKGCMC | 1 cut(s) | 2524 |
| BstUI | CGCG | 1 cut(s) | 860 |
| BstV1I | GCAGC | 6 cut(s) | 801, 882, 1288, 1469, 1830, 2705 |
| BstV2I | GAAGAC | 2 cut(s) | 2290, 2598 |
| BstX2I | RGATCY | 1 cut(s) | 1093 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1705 |
| BstYI | RGATCY | 1 cut(s) | 1093 |
| BsuI | GTATCC | 1 cut(s) | 1320 |
| BsuRI | GGCC | 7 cut(s) | 516, 1477, 1723, 1957, 1965, 2212, 2685 |
| BtgZI | GCGATG | 1 cut(s) | 1402 |
| BtsIMutI | CAGTG | 8 cut(s) | 543, 1059, 1192, 1218, 1301, 1638, 1864, 2536 |
| BveI | ACCTGC | 1 cut(s) | 991 |
| Cac8I | GCNNGC | 3 cut(s) | 1585, 2409, 2491 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1463, 1637 |
| Cfr10I | RCCGGY | 1 cut(s) | 1085 |
| Cfr13I | GGNCC | 2 cut(s) | 515, 1964 |
| Cfr9I | CCCGGG | 1 cut(s) | 2516 |
| CseI | GACGC | 1 cut(s) | 2366 |
| Csp6I | GTAC | 9 cut(s) | 105, 727, 775, 850, 1123, 1886, 2029, 2443, 2455 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 602, 637 |
| CviQI | GTAC | 9 cut(s) | 105, 727, 775, 850, 1123, 1886, 2029, 2443, 2455 |
| DraI | TTTAAA | 1 cut(s) | 660 |
| DraIII | CACNNNGTG | 2 cut(s) | 572, 1296 |
| Eam1104I | CTCTTC | 2 cut(s) | 1337, 2663 |
| EarI | CTCTTC | 2 cut(s) | 1337, 2663 |
| Ecl136II | GAGCTC | 3 cut(s) | 652, 1532, 2158 |
| Eco130I | CCWWGG | 3 cut(s) | 1718, 1993, 2535 |
| Eco147I | AGGCCT | 3 cut(s) | 1723, 2212, 2685 |
| Eco24I | GRGCYC | 5 cut(s) | 191, 644, 654, 1534, 2160 |
| Eco32I | GATATC | 3 cut(s) | 979, 1237, 2074 |
| Eco53kI | GAGCTC | 3 cut(s) | 652, 1532, 2158 |
| Eco88I | CYCGRG | 2 cut(s) | 1426, 2516 |
| EcoICRI | GAGCTC | 3 cut(s) | 652, 1532, 2158 |
| EcoO109I | RGGNCCY | 1 cut(s) | 515 |
| EcoRI | GAATTC | 1 cut(s) | 2321 |
| EcoRII | CCWGG | 4 cut(s) | 799, 2390, 2484, 2611 |
| EcoRV | GATATC | 3 cut(s) | 979, 1237, 2074 |
| EcoT14I | CCWWGG | 3 cut(s) | 1718, 1993, 2535 |
| EcoT38I | GRGCYC | 5 cut(s) | 191, 644, 654, 1534, 2160 |
| ErhI | CCWWGG | 3 cut(s) | 1718, 1993, 2535 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 753, 785, 1986, 2018, 2579, 2611 |
| FaqI | GGGAC | 2 cut(s) | 853, 2131 |
| FauI | CCCGC | 1 cut(s) | 829 |
| FauNDI | CATATG | 1 cut(s) | 2508 |
| FbaI | TGATCA | 5 cut(s) | 402, 1255, 1599, 1626, 2758 |
| FblI | GTMKAC | 2 cut(s) | 1394, 2331 |
| Fnu4HI | GCNGC | 6 cut(s) | 815, 871, 1302, 1458, 1844, 2694 |
| FriOI | GRGCYC | 5 cut(s) | 191, 644, 654, 1534, 2160 |
| Fsp4HI | GCNGC | 6 cut(s) | 815, 871, 1302, 1458, 1844, 2694 |
| FspBI | CTAG | 3 cut(s) | 114, 1584, 1937 |
| GluI | GCNGC | 6 cut(s) | 815, 871, 1302, 1458, 1844, 2694 |
| GsuI | CTGGAG | 1 cut(s) | 2613 |
| HaeIII | GGCC | 7 cut(s) | 516, 1477, 1723, 1957, 1965, 2212, 2685 |
| HapII | CCGG | 3 cut(s) | 1086, 2517, 2697 |
| HgaI | GACGC | 1 cut(s) | 2366 |
| Hin1I | GRCGYC | 1 cut(s) | 2358 |
| HincII | GTYRAC | 1 cut(s) | 1156 |
| HindII | GTYRAC | 1 cut(s) | 1156 |
| HindIII | AAGCTT | 4 cut(s) | 697, 1410, 2409, 2491 |
| HinfI | GANTC | 7 cut(s) | 296, 686, 796, 1157, 1986, 2221, 2346 |
| HpaII | CCGG | 3 cut(s) | 1086, 2517, 2697 |
| HphI | GGTGA | 8 cut(s) | 1066, 1193, 1636, 1804, 1889, 2212, 2341, 2560 |
| Hpy166II | GTNNAC | 7 cut(s) | 775, 1006, 1156, 1190, 1395, 1828, 2332 |
| Hpy188I | TCNGA | 9 cut(s) | 399, 1213, 1250, 1555, 2041, 2450, 2472, 2566, 2679 |
| Hpy8I | GTNNAC | 7 cut(s) | 775, 1006, 1156, 1190, 1395, 1828, 2332 |
| Hpy99I | CGWCG | 1 cut(s) | 2048 |
| HpyCH4III | ACNGT | 6 cut(s) | 538, 1187, 1222, 1399, 1633, 1910 |
| HpyCH4IV | ACGT | 1 cut(s) | 2457 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 161, 895, 958, 1418, 1440, 1463, 1529, 2120, 2699 |
| HpySE526I | ACGT | 1 cut(s) | 2457 |
| Hsp92I | GRCGYC | 1 cut(s) | 2358 |
| KpnI | GGTACC | 2 cut(s) | 1889, 2032 |
| Ksp22I | TGATCA | 5 cut(s) | 402, 1255, 1599, 1626, 2758 |
| LmnI | GCTCC | 2 cut(s) | 639, 1529 |
| Lsp1109I | GCAGC | 6 cut(s) | 801, 882, 1288, 1469, 1830, 2705 |
| LweI | GCATC | 6 cut(s) | 382, 1315, 1444, 1852, 2642, 2717 |
| MaeI | CTAG | 3 cut(s) | 114, 1584, 1937 |
| MaeII | ACGT | 1 cut(s) | 2457 |
| MaeIII | GTNAC | 7 cut(s) | 121, 532, 825, 1181, 1283, 2058, 2274 |
| MflI | RGATCY | 1 cut(s) | 1093 |
| MhlI | GDGCHC | 8 cut(s) | 191, 357, 577, 644, 654, 1534, 2160, 2524 |
| MlyI | GAGTC | 1 cut(s) | 1151 |
| MmeI | TCCRAC | 3 cut(s) | 292, 292, 2249 |
| MroXI | GAANNNNTTC | 3 cut(s) | 324, 2423, 2657 |
| MseI | TTAA | 6 cut(s) | 659, 1359, 1506, 1644, 1899, 2797 |
| MslI | CAYNNNNRTG | 5 cut(s) | 247, 2339, 2435, 2529, 2782 |
| MspA1I | CMGCKG | 2 cut(s) | 481, 580 |
| MspI | CCGG | 3 cut(s) | 1086, 2517, 2697 |
| MspR9I | CCNGG | 7 cut(s) | 801, 2392, 2486, 2517, 2518, 2613, 2698 |
| Mva1269I | GAATGC | 3 cut(s) | 60, 1699, 1794 |
| MvaI | CCWGG | 4 cut(s) | 801, 2392, 2486, 2613 |
| MvnI | CGCG | 1 cut(s) | 860 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 161, 895, 958, 1418, 1440, 1463, 1529, 2120, 2699 |
| NciI | CCSGG | 3 cut(s) | 2517, 2518, 2698 |
| NdeI | CATATG | 1 cut(s) | 2508 |
| NheI | GCTAGC | 1 cut(s) | 1583 |
| NmuCI | GTSAC | 3 cut(s) | 532, 825, 1181 |
| NspI | RCATGY | 1 cut(s) | 833 |
| NspV | TTCGAA | 2 cut(s) | 23, 1733 |
| PaqCI | CACCTGC | 1 cut(s) | 991 |
| PceI | AGGCCT | 3 cut(s) | 1723, 2212, 2685 |
| PciI | ACATGT | 1 cut(s) | 829 |
| PctI | GAATGC | 3 cut(s) | 60, 1699, 1794 |
| PdmI | GAANNNNTTC | 3 cut(s) | 324, 2423, 2657 |
| PfeI | GAWTC | 6 cut(s) | 296, 686, 796, 1986, 2221, 2346 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1023, 2313 |
| PfoI | TCCNGGA | 1 cut(s) | 2390 |
| PkrI | GCNGC | 6 cut(s) | 816, 872, 1303, 1459, 1845, 2695 |
| Ple19I | CGATCG | 1 cut(s) | 198 |
| PleI | GAGTC | 1 cut(s) | 1151 |
| PpsI | GAGTC | 1 cut(s) | 1151 |
| PscI | ACATGT | 1 cut(s) | 829 |
| PshBI | ATTAAT | 1 cut(s) | 1644 |
| Psp124BI | GAGCTC | 3 cut(s) | 654, 1534, 2160 |
| Psp6I | CCWGG | 4 cut(s) | 799, 2390, 2484, 2611 |
| PspGI | CCWGG | 4 cut(s) | 799, 2390, 2484, 2611 |
| PspPI | GGNCC | 2 cut(s) | 515, 1964 |
| PstI | CTGCAG | 3 cut(s) | 819, 1039, 2285 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1463, 1637 |
| PsuI | RGATCY | 1 cut(s) | 1093 |
| PvuI | CGATCG | 1 cut(s) | 198 |
| PvuII | CAGCTG | 2 cut(s) | 481, 580 |
| RsaI | GTAC | 9 cut(s) | 106, 728, 776, 851, 1124, 1887, 2030, 2444, 2456 |
| RsaNI | GTAC | 9 cut(s) | 105, 727, 775, 850, 1123, 1886, 2029, 2443, 2455 |
| RseI | CAYNNNNRTG | 5 cut(s) | 247, 2339, 2435, 2529, 2782 |
| SacI | GAGCTC | 3 cut(s) | 654, 1534, 2160 |
| SaqAI | TTAA | 6 cut(s) | 659, 1359, 1506, 1644, 1899, 2797 |
| SatI | GCNGC | 6 cut(s) | 815, 871, 1302, 1458, 1844, 2694 |
| Sau96I | GGNCC | 2 cut(s) | 515, 1964 |
| ScaI | AGTACT | 2 cut(s) | 106, 728 |
| SchI | GAGTC | 1 cut(s) | 1151 |
| ScrFI | CCNGG | 7 cut(s) | 801, 2392, 2486, 2517, 2518, 2613, 2698 |
| SduI | GDGCHC | 8 cut(s) | 191, 357, 577, 644, 654, 1534, 2160, 2524 |
| SfaNI | GCATC | 6 cut(s) | 382, 1315, 1444, 1852, 2642, 2717 |
| SfcI | CTRYAG | 4 cut(s) | 815, 922, 1035, 2281 |
| SfuI | TTCGAA | 2 cut(s) | 23, 1733 |
| SmaI | CCCGGG | 1 cut(s) | 2518 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 247, 2339, 2435, 2529, 2782 |
| SmlI | CTYRAG | 4 cut(s) | 410, 1379, 1469, 2063 |
| SmoI | CTYRAG | 4 cut(s) | 410, 1379, 1469, 2063 |
| SpeI | ACTAGT | 1 cut(s) | 1936 |
| SseBI | AGGCCT | 3 cut(s) | 1723, 2212, 2685 |
| SsiI | CCGC | 4 cut(s) | 300, 836, 860, 1443 |
| SspI | AATATT | 1 cut(s) | 1321 |
| SspMI | CTAG | 3 cut(s) | 114, 1584, 1937 |
| SstI | GAGCTC | 3 cut(s) | 654, 1534, 2160 |
| StuI | AGGCCT | 3 cut(s) | 1723, 2212, 2685 |
| StyD4I | CCNGG | 7 cut(s) | 799, 2390, 2484, 2515, 2516, 2611, 2696 |
| StyI | CCWWGG | 3 cut(s) | 1718, 1993, 2535 |
| TaaI | ACNGT | 6 cut(s) | 538, 1187, 1222, 1399, 1633, 1910 |
| TaiI | ACGT | 1 cut(s) | 2460 |
| TatI | WGTACW | 5 cut(s) | 104, 726, 774, 849, 1122 |
| TfiI | GAWTC | 6 cut(s) | 296, 686, 796, 1986, 2221, 2346 |
| Tru1I | TTAA | 6 cut(s) | 659, 1359, 1506, 1644, 1899, 2797 |
| Tru9I | TTAA | 6 cut(s) | 659, 1359, 1506, 1644, 1899, 2797 |
| TscAI | CASTG | 8 cut(s) | 543, 1066, 1192, 1225, 1301, 1638, 1864, 2536 |
| TseFI | GTSAC | 3 cut(s) | 532, 825, 1181 |
| TseI | GCWGC | 6 cut(s) | 814, 870, 1301, 1457, 1843, 2693 |
| Tsp45I | GTSAC | 3 cut(s) | 532, 825, 1181 |
| TspGWI | ACGGA | 2 cut(s) | 857, 1336 |
| TspMI | CCCGGG | 1 cut(s) | 2516 |
| TspRI | CASTG | 8 cut(s) | 543, 1066, 1192, 1225, 1301, 1638, 1864, 2536 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1023, 2313 |
| VspI | ATTAAT | 1 cut(s) | 1644 |
| XapI | RAATTY | 5 cut(s) | 19, 1385, 1605, 2321, 2792 |
| XceI | RCATGY | 1 cut(s) | 833 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 222, 2542 |
| XmaI | CCCGGG | 1 cut(s) | 2516 |
| XmiI | GTMKAC | 2 cut(s) | 1394, 2331 |
| XmnI | GAANNNNTTC | 3 cut(s) | 324, 2423, 2657 |
| XspI | CTAG | 3 cut(s) | 114, 1584, 1937 |
| ZrmI | AGTACT | 2 cut(s) | 106, 728 |