Rorug05G0317100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0013531
Physical Location & Seq
Forward (+)
37709472 .. 37715319
5848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0317100.1

Sequence Viewer

Length: 1182 bp
ATGGCAAGCTTCACCAACACCGTCTTATTCAGAATCATTCTGGTCCTTGTTAGTCTCTCAGATTCAGCTCTGTTCCATGTGCGGTGCTTAAGCTTCGGCATCAACTACGGCCAAATCGCCAACAACCTCCCTCCTCCTTCCCAAGTAGCGGGGCTCCTCCAGTCCCTCAACGTCAGCAGAGTCAAACTCTACGACGCCGATCCCAATGTCCTCCAAGCCTTCTCCAACTCCGAGGTCGACTTCATCATCGAACTCGGAAACGAGCTCCTACCCAACATGACCGACCCGCTCAAGGCCCAGGCTTGGATCCAACAACACGTCACCCCTCACCTCCCGCAGACCAAAATCTCCTCCATCCTCGTCGGCAACGAGATCCTCGGCGGAACCGACACCCGTCTCATGTCGTACCTCCTCATCGCAATGCAATCCGTCTACGCCGCCCTCGTCAACCTCGGACTTTCCAAAACCATCGTCGTCACCAGCGCGCATTCCCTTGCCATTTTGGGAAACTCCTACCCGCCGTCGGCCGGGACTTTCCAGGAAAACCTCGCCCAATACATCAAGCCAATTCTAGACTTCCACTCGCAGACCAATGCCCCGTTCCTCGTAAACGTGTACCCTTACTTTGCTTACAAGGGCAGTCCTGATGAAGTTTCCTTGGAGTACGTGCTTTTTCAGCCAAACTCCGGCATGAATGATTCCGGCACCAATCTTCACTACGATAACATGCTCGATGCTCAGATTGACGCTGTCTATGCCGCAATGAAAGTGATGGGCCACACTGATATCGAGGTTCGGATATCTGAAACCGGCTGGCCGTCCAACGGAGACGAGGATGAAGCCGGAGCCACGCCGGAGAATGCCGGGATTTACAATGGGAATTTGCTCAGGAAAATTGTGGAGAAGAAAGGCACTCCGGCCAAGCCTGACGTTCCTATTGATACATATGTGTTTGCTCTGTTTAATGAGGACTTGAAGCCCGGTCCGGCGTCGGAGAGGAACTACGGGCTGTTTTATCCCAATGGGACTCGTGTGTATGATATGGGAGATGTCGGAGCTGCCGGACCTGACGGAGCAGATGGTTATCTTCCCCAGTTTACTTTTTCTTCAGCTCATGATATTAATGCTTTGTCGATCTTCAATTTTCTGATATTTCTTGTAGTGTACTTATTATGGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

393

Amino Acids

42.99

Weight (kDa)

4.65

Isoelectric Point (pI)

31.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 33 - 348 1.3e-85 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013531)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60700 AT5G60700
fragaria_vesca FvH4_3g31890 FvH4_3g31890
malus_domestica MD03G1120400.v1.1 MD11G1138800.v1.1
prunus_persica Prupe.6G103800_v2.0.a1
pyrus_communis pycom11g11450
rosa_chinensis RchiOBHm_Chr5g0058661
rosa_laevigata RLG00000035238
rosa_multiflora Rmu_sc0000367.1_g000205
rosa_roxburghii Rroxscaffold_1G00021760
rosa_rugosa Rorug05G0317100
rosa_samantha Rh5AG382800 Rh5BG394700 Rh5CG418500 Rh5DG410100
rosa_wichuraiana Rw5G036070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 704
AccBSI CCGCTC 1 cut(s) 289
AccI GTMKAC 2 cut(s) 237, 432
AccII CGCG 1 cut(s) 485
AciI CCGC 8 cut(s) 82, 149, 287, 335, 381, 438, 518, 759
AclWI GGATC 4 cut(s) 194, 301, 314, 367
AcoI YGGCCR 4 cut(s) 109, 525, 815, 918
AcsI RAATTY 1 cut(s) 880
AcuI CTGAAG 1 cut(s) 1092
AcyI GRCGYC 2 cut(s) 195, 989
AfaI GTAC 4 cut(s) 407, 617, 665, 1166
AfiI CCNNNNNNNGG 7 cut(s) 148, 292, 303, 523, 527, 686, 824
AflII CTTAAG 1 cut(s) 88
AflIII ACRYGT 2 cut(s) 316, 612
AgsI TTSAA 2 cut(s) 976, 1141
AjiI CACGTC 1 cut(s) 319
AjnI CCWGG 2 cut(s) 297, 537
AjuI GAANNNNNNNTTGG 2 cut(s) 584, 616
AluBI AGCT 6 cut(s) 9, 68, 93, 265, 1058, 1112
AluI AGCT 6 cut(s) 9, 68, 93, 265, 1058, 1112
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 3 cut(s) 59, 401, 822
AlwI GGATC 4 cut(s) 194, 301, 314, 367
AoxI GGCC 6 cut(s) 109, 294, 525, 775, 815, 918
ApeKI GCWGC 1 cut(s) 1058
ApoI RAATTY 1 cut(s) 880
ArsI GACNNNNNNTTYG 2 cut(s) 456, 488
AseI ATTAAT 1 cut(s) 1122
AspLEI GCGC 2 cut(s) 485, 487
AspS9I GGNCC 5 cut(s) 43, 295, 775, 983, 1064
AsuC2I CCSGG 3 cut(s) 529, 865, 981
AsuHPI GGTGA 4 cut(s) 4, 313, 320, 469
AvaII GGWCC 3 cut(s) 43, 983, 1064
BaeI ACNNNNGTAYC 2 cut(s) 933, 966
BamHI GGATCC 1 cut(s) 306
BanI GGYRCC 1 cut(s) 704
BanII GRGCYC 2 cut(s) 156, 267
BauI CACGAG 1 cut(s) 1029
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 1 cut(s) 1045
BccI CCATC 4 cut(s) 362, 476, 766, 1073
BceAI ACGGC 3 cut(s) 124, 505, 802
BciT130I CCWGG 2 cut(s) 299, 539
BcnI CCSGG 3 cut(s) 529, 865, 981
BcoDI GTCTC 3 cut(s) 59, 401, 822
BfaI CTAG 1 cut(s) 572
BfrI CTTAAG 1 cut(s) 88
BisI GCNGC 3 cut(s) 438, 759, 1059
BlsI GCNGC 3 cut(s) 439, 760, 1060
Bme1390I CCNGG 5 cut(s) 299, 529, 539, 865, 981
Bme18I GGWCC 3 cut(s) 43, 983, 1064
BmgBI CACGTC 1 cut(s) 319
BmgT120I GGNCC 5 cut(s) 43, 295, 775, 983, 1064
BmiI GGNNCC 5 cut(s) 155, 308, 385, 706, 847
BmrFI CCNGG 5 cut(s) 299, 529, 539, 865, 981
BmrI ACTGGG 1 cut(s) 1087
BmsI GCATC 2 cut(s) 108, 724
BmuI ACTGGG 1 cut(s) 1087
BoxI GACNNNNGTC 1 cut(s) 393
BplI GAGNNNNNCTC 2 cut(s) 171, 203
BpmI CTGGAG 1 cut(s) 143
Bpu10I CCTNAGC 1 cut(s) 887
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 3 cut(s) 529, 865, 981
BsaAI YACGTR 1 cut(s) 667
BsaBI GATNNNNATC 1 cut(s) 1083
BsaHI GRCGYC 2 cut(s) 195, 989
BsaJI CCNNGG 5 cut(s) 231, 297, 376, 451, 657
Bsc4I CCNNNNNNNGG 7 cut(s) 148, 292, 303, 523, 527, 686, 824
Bse118I RCCGGY 1 cut(s) 809
Bse1I ACTGG 2 cut(s) 160, 1093
Bse3DI GCAATG 2 cut(s) 426, 768
Bse8I GATNNNNATC 1 cut(s) 1083
BseBI CCWGG 2 cut(s) 299, 539
BseDI CCNNGG 5 cut(s) 231, 297, 376, 451, 657
BseGI GGATG 2 cut(s) 354, 841
BseJI GATNNNNATC 1 cut(s) 1083
BseLI CCNNNNNNNGG 7 cut(s) 148, 292, 303, 523, 527, 686, 824
BseMI GCAATG 2 cut(s) 426, 768
BseMII CTCAG 3 cut(s) 72, 752, 901
BseNI ACTGG 2 cut(s) 160, 1093
BsePI GCGCGC 1 cut(s) 483
BseRI GAGGAG 4 cut(s) 123, 146, 340, 401
BseX3I CGGCCG 1 cut(s) 525
BseXI GCAGC 1 cut(s) 1045
Bsh1236I CGCG 1 cut(s) 485
Bsh1285I CGRYCG 1 cut(s) 528
BshFI GGCC 6 cut(s) 111, 296, 527, 777, 817, 920
BshNI GGYRCC 1 cut(s) 704
BsiEI CGRYCG 1 cut(s) 528
BsiHKAI GWGCWC 1 cut(s) 267
BslFI GGGAC 3 cut(s) 148, 544, 1039
BslI CCNNNNNNNGG 7 cut(s) 148, 292, 303, 523, 527, 686, 824
BsmAI GTCTC 3 cut(s) 59, 401, 822
BsmBI CGTCTC 2 cut(s) 401, 822
BsmFI GGGAC 3 cut(s) 148, 544, 1039
BsmI GAATGC 2 cut(s) 487, 865
BsnI GGCC 6 cut(s) 111, 296, 527, 777, 817, 920
Bsp1286I GDGCHC 2 cut(s) 156, 267
Bsp143I GATC 4 cut(s) 199, 306, 372, 1134
BspACI CCGC 8 cut(s) 82, 149, 287, 335, 381, 438, 518, 759
BspANI GGCC 6 cut(s) 111, 296, 527, 777, 817, 920
BspCNI CTCAG 3 cut(s) 71, 751, 900
BspFNI CGCG 1 cut(s) 485
BspHI TCATGA 1 cut(s) 1114
BspLI GGNNCC 5 cut(s) 155, 308, 385, 706, 847
BspPI GGATC 4 cut(s) 194, 301, 314, 367
BspT107I GGYRCC 1 cut(s) 704
BspTI CTTAAG 1 cut(s) 88
BsrBI CCGCTC 1 cut(s) 289
BsrDI GCAATG 2 cut(s) 426, 768
BsrFI RCCGGY 1 cut(s) 809
BsrI ACTGG 2 cut(s) 160, 1093
BssAI RCCGGY 1 cut(s) 809
BssECI CCNNGG 5 cut(s) 231, 297, 376, 451, 657
BssHII GCGCGC 1 cut(s) 483
BssMI GATC 4 cut(s) 199, 306, 372, 1134
BssNI GRCGYC 2 cut(s) 195, 989
BssSI CACGAG 1 cut(s) 1029
BssT1I CCWWGG 1 cut(s) 657
Bst2BI CACGAG 1 cut(s) 1029
Bst2UI CCWGG 2 cut(s) 299, 539
Bst4CI ACNGT 1 cut(s) 22
BstACI GRCGYC 2 cut(s) 195, 989
BstAFI CTTAAG 1 cut(s) 88
BstBAI YACGTR 1 cut(s) 667
BstC8I GCNNGC 3 cut(s) 7, 485, 815
BstDEI CTNAG 3 cut(s) 58, 738, 887
BstF5I GGATG 2 cut(s) 354, 841
BstFNI CGCG 1 cut(s) 485
BstHHI GCGC 2 cut(s) 485, 487
BstKTI GATC 4 cut(s) 202, 309, 375, 1137
BstMAI GTCTC 3 cut(s) 59, 401, 822
BstMBI GATC 4 cut(s) 199, 306, 372, 1134
BstMCI CGRYCG 1 cut(s) 528
BstMWI GCNNNNNNNGC 2 cut(s) 676, 755
BstNI CCWGG 2 cut(s) 299, 539
BstNSI RCATGY 1 cut(s) 730
BstPAI GACNNNNGTC 1 cut(s) 393
BstSCI CCNGG 5 cut(s) 297, 527, 537, 863, 979
BstUI CGCG 1 cut(s) 485
BstV1I GCAGC 1 cut(s) 1045
BstX2I RGATCY 2 cut(s) 306, 372
BstYI RGATCY 2 cut(s) 306, 372
BstZI CGGCCG 1 cut(s) 525
BsuRI GGCC 6 cut(s) 111, 296, 527, 777, 817, 920
BtgZI GCGATG 1 cut(s) 400
BtrI CACGTC 1 cut(s) 319
BtsCI GGATG 2 cut(s) 354, 841
BtsIMutI CAGTG 1 cut(s) 780
Cac8I GCNNGC 3 cut(s) 7, 485, 815
CciI TCATGA 1 cut(s) 1114
CfoI GCGC 2 cut(s) 485, 487
Cfr10I RCCGGY 1 cut(s) 809
Cfr13I GGNCC 5 cut(s) 43, 295, 775, 983, 1064
CpoI CGGWCCG 1 cut(s) 983
CseI GACGC 3 cut(s) 203, 755, 978
Csp6I GTAC 4 cut(s) 406, 616, 664, 1165
CspI CGGWCCG 1 cut(s) 983
CviAII CATG 6 cut(s) 77, 277, 400, 691, 727, 1115
CviQI GTAC 4 cut(s) 406, 616, 664, 1165
DdeI CTNAG 3 cut(s) 58, 738, 887
DpnI GATC 4 cut(s) 201, 308, 374, 1136
DpnII GATC 4 cut(s) 199, 306, 372, 1134
EaeI YGGCCR 4 cut(s) 109, 525, 815, 918
EagI CGGCCG 1 cut(s) 525
EciI GGCGGA 1 cut(s) 396
Ecl136II GAGCTC 1 cut(s) 265
EclXI CGGCCG 1 cut(s) 525
Eco130I CCWWGG 1 cut(s) 657
Eco24I GRGCYC 2 cut(s) 156, 267
Eco32I GATATC 2 cut(s) 787, 801
Eco47I GGWCC 3 cut(s) 43, 983, 1064
Eco52I CGGCCG 1 cut(s) 525
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 1092
EcoICRI GAGCTC 1 cut(s) 265
EcoRII CCWGG 2 cut(s) 297, 537
EcoRV GATATC 2 cut(s) 787, 801
EcoT14I CCWWGG 1 cut(s) 657
EcoT38I GRGCYC 2 cut(s) 156, 267
ErhI CCWWGG 1 cut(s) 657
Esp3I CGTCTC 2 cut(s) 401, 822
FaeI CATG 6 cut(s) 80, 280, 403, 694, 730, 1118
FaqI GGGAC 3 cut(s) 148, 544, 1039
FatI CATG 6 cut(s) 76, 276, 399, 690, 726, 1114
FauI CCCGC 4 cut(s) 142, 294, 342, 525
FauNDI CATATG 1 cut(s) 946
FblI GTMKAC 2 cut(s) 237, 432
Fnu4HI GCNGC 3 cut(s) 438, 759, 1059
FokI GGATG 2 cut(s) 341, 848
FriOI GRGCYC 2 cut(s) 156, 267
Fsp4HI GCNGC 3 cut(s) 438, 759, 1059
FspBI CTAG 1 cut(s) 572
GlaI GCGC 2 cut(s) 484, 486
GluI GCNGC 3 cut(s) 438, 759, 1059
GsuI CTGGAG 1 cut(s) 143
HaeIII GGCC 6 cut(s) 111, 296, 527, 777, 817, 920
HgaI GACGC 3 cut(s) 203, 755, 978
HhaI GCGC 2 cut(s) 485, 487
Hin1I GRCGYC 2 cut(s) 195, 989
Hin1II CATG 6 cut(s) 80, 280, 403, 694, 730, 1118
Hin6I GCGC 2 cut(s) 483, 485
HinP1I GCGC 2 cut(s) 483, 485
HincII GTYRAC 2 cut(s) 238, 448
HindII GTYRAC 2 cut(s) 238, 448
HindIII AAGCTT 2 cut(s) 7, 91
HinfI GANTC 5 cut(s) 33, 62, 180, 698, 1027
HphI GGTGA 4 cut(s) 4, 313, 320, 469
Hpy166II GTNNAC 7 cut(s) 238, 433, 448, 610, 616, 1098, 1165
Hpy188III TCNNGA 4 cut(s) 572, 644, 889, 1115
Hpy8I GTNNAC 7 cut(s) 238, 433, 448, 610, 616, 1098, 1165
Hpy99I CGWCG 5 cut(s) 197, 365, 476, 526, 994
HpyAV CCTTC 2 cut(s) 147, 229
HpyCH4III ACNGT 1 cut(s) 22
HpyCH4IV ACGT 5 cut(s) 171, 318, 612, 666, 930
HpyCH4V TGCA 1 cut(s) 424
HpyF10VI GCNNNNNNNGC 2 cut(s) 676, 755
HpyF3I CTNAG 3 cut(s) 58, 738, 887
HpySE526I ACGT 5 cut(s) 171, 318, 612, 666, 930
Hsp92I GRCGYC 2 cut(s) 195, 989
Hsp92II CATG 6 cut(s) 80, 280, 403, 694, 730, 1118
HspAI GCGC 2 cut(s) 483, 485
Kzo9I GATC 4 cut(s) 199, 306, 372, 1134
LmnI GCTCC 5 cut(s) 159, 270, 845, 1055, 1073
Lsp1109I GCAGC 1 cut(s) 1045
LweI GCATC 2 cut(s) 108, 724
MaeI CTAG 1 cut(s) 572
MaeII ACGT 5 cut(s) 171, 318, 612, 666, 930
MaeIII GTNAC 2 cut(s) 319, 475
MalI GATC 4 cut(s) 201, 308, 374, 1136
MbiI CCGCTC 1 cut(s) 289
MboI GATC 4 cut(s) 199, 306, 372, 1134
MboII GAAGA 5 cut(s) 704, 916, 1079, 1098, 1129
MflI RGATCY 2 cut(s) 306, 372
MhlI GDGCHC 2 cut(s) 156, 267
MluCI AATT 4 cut(s) 567, 880, 894, 1141
MlyI GAGTC 2 cut(s) 189, 1021
MmeI TCCRAC 5 cut(s) 249, 334, 846, 972, 1033
MseI TTAA 3 cut(s) 89, 963, 1122
MslI CAYNNNNRTG 1 cut(s) 419
MspCI CTTAAG 1 cut(s) 88
MspR9I CCNGG 5 cut(s) 299, 529, 539, 865, 981
Mva1269I GAATGC 2 cut(s) 487, 865
MvaI CCWGG 2 cut(s) 299, 539
MvnI CGCG 1 cut(s) 485
MwoI GCNNNNNNNGC 2 cut(s) 676, 755
NciI CCSGG 3 cut(s) 529, 865, 981
NdeI CATATG 1 cut(s) 946
NdeII GATC 4 cut(s) 199, 306, 372, 1134
NlaIII CATG 6 cut(s) 80, 280, 403, 694, 730, 1118
NlaIV GGNNCC 5 cut(s) 155, 308, 385, 706, 847
NmeAIII GCCGAG 1 cut(s) 357
NmuCI GTSAC 2 cut(s) 319, 475
NspI RCATGY 1 cut(s) 730
PagI TCATGA 1 cut(s) 1114
PauI GCGCGC 1 cut(s) 483
PcsI WCGNNNNNNNCGW 3 cut(s) 366, 384, 441
PctI GAATGC 2 cut(s) 487, 865
PfeI GAWTC 3 cut(s) 33, 62, 698
PflFI GACNNNGTC 1 cut(s) 749
PfoI TCCNGGA 1 cut(s) 537
PkrI GCNGC 3 cut(s) 439, 760, 1060
PleI GAGTC 2 cut(s) 188, 1021
PpsI GAGTC 2 cut(s) 188, 1021
Ppu21I YACGTR 1 cut(s) 667
PshAI GACNNNNGTC 1 cut(s) 393
PshBI ATTAAT 1 cut(s) 1122
Psp124BI GAGCTC 1 cut(s) 267
Psp6I CCWGG 2 cut(s) 297, 537
PspGI CCWGG 2 cut(s) 297, 537
PspN4I GGNNCC 5 cut(s) 155, 308, 385, 706, 847
PspPI GGNCC 5 cut(s) 43, 295, 775, 983, 1064
PsuI RGATCY 2 cut(s) 306, 372
PsyI GACNNNGTC 1 cut(s) 749
PteI GCGCGC 1 cut(s) 483
RsaI GTAC 4 cut(s) 407, 617, 665, 1166
RsaNI GTAC 4 cut(s) 406, 616, 664, 1165
RseI CAYNNNNRTG 1 cut(s) 419
Rsr2I CGGWCCG 1 cut(s) 983
RsrII CGGWCCG 1 cut(s) 983
SacI GAGCTC 1 cut(s) 267
SalI GTCGAC 1 cut(s) 236
SaqAI TTAA 3 cut(s) 89, 963, 1122
SatI GCNGC 3 cut(s) 438, 759, 1059
Sau3AI GATC 4 cut(s) 199, 306, 372, 1134
Sau96I GGNCC 5 cut(s) 43, 295, 775, 983, 1064
SchI GAGTC 2 cut(s) 189, 1021
ScrFI CCNGG 5 cut(s) 299, 529, 539, 865, 981
SduI GDGCHC 2 cut(s) 156, 267
SfaNI GCATC 2 cut(s) 108, 724
SinI GGWCC 3 cut(s) 43, 983, 1064
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 2 cut(s) 88, 290
SmoI CTYRAG 2 cut(s) 88, 290
Sse9I AATT 4 cut(s) 567, 880, 894, 1141
SsiI CCGC 8 cut(s) 82, 149, 287, 335, 381, 438, 518, 759
SspMI CTAG 1 cut(s) 572
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 5 cut(s) 297, 527, 537, 863, 979
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 1 cut(s) 22
TaiI ACGT 5 cut(s) 174, 321, 615, 669, 933
TaqI TCGA 5 cut(s) 237, 249, 732, 789, 1133
TaqII GACCGA 1 cut(s) 296
TasI AATT 4 cut(s) 567, 880, 894, 1141
TatI WGTACW 1 cut(s) 1164
TauI GCSGC 2 cut(s) 440, 761
TfiI GAWTC 3 cut(s) 33, 62, 698
Tru1I TTAA 3 cut(s) 89, 963, 1122
Tru9I TTAA 3 cut(s) 89, 963, 1122
TscAI CASTG 1 cut(s) 787
TseFI GTSAC 2 cut(s) 319, 475
TseI GCWGC 1 cut(s) 1058
Tsp45I GTSAC 2 cut(s) 319, 475
TspDTI ATGAA 5 cut(s) 232, 663, 707, 779, 852
TspGWI ACGGA 3 cut(s) 418, 840, 1086
TspRI CASTG 1 cut(s) 787
Tth111I GACNNNGTC 1 cut(s) 749
Vha464I CTTAAG 1 cut(s) 88
VpaK11BI GGWCC 3 cut(s) 43, 983, 1064
VspI ATTAAT 1 cut(s) 1122
XapI RAATTY 1 cut(s) 880
XbaI TCTAGA 1 cut(s) 571
XceI RCATGY 1 cut(s) 730
XmiI GTMKAC 2 cut(s) 237, 432
XspI CTAG 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.