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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G60700 AT5G60700 |
| fragaria_vesca | FvH4_3g31890 FvH4_3g31890 |
| malus_domestica | MD03G1120400.v1.1 MD11G1138800.v1.1 |
| prunus_persica | Prupe.6G103800_v2.0.a1 |
| pyrus_communis | pycom11g11450 |
| rosa_chinensis | RchiOBHm_Chr5g0058661 |
| rosa_laevigata | RLG00000035238 |
| rosa_multiflora | Rmu_sc0000367.1_g000205 |
| rosa_roxburghii | Rroxscaffold_1G00021760 |
| rosa_rugosa | Rorug05G0317100 |
| rosa_samantha | Rh5AG382800 Rh5BG394700 Rh5CG418500 Rh5DG410100 |
| rosa_wichuraiana | Rw5G036070 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 994 |
| Acc36I | ACCTGC | 1 cut(s) | 994 |
| Acc65I | GGTACC | 2 cut(s) | 1888, 2031 |
| AccB1I | GGYRCC | 2 cut(s) | 1888, 2031 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1026, 2316 |
| AccBSI | CCGCTC | 1 cut(s) | 1501 |
| AccI | GTMKAC | 2 cut(s) | 675, 2776 |
| AciI | CCGC | 2 cut(s) | 1501, 1795 |
| AclWI | GGATC | 5 cut(s) | 48, 1643, 1997, 2114, 2132 |
| AcoI | YGGCCR | 1 cut(s) | 318 |
| AcsI | RAATTY | 5 cut(s) | 348, 1388, 1665, 2382, 2605 |
| AcuI | CTGAAG | 1 cut(s) | 434 |
| AdeI | CACNNNGTG | 3 cut(s) | 575, 1299, 2789 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 625, 1026, 1624, 1824, 1825, 1842, 2316, 2712 |
| AgeI | ACCGGT | 1 cut(s) | 2771 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 533 |
| AjnI | CCWGG | 5 cut(s) | 802, 2393, 2487, 2614, 2792 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 203, 235, 591, 623, 851, 883 |
| AleI | CACNNNNGTG | 1 cut(s) | 1474 |
| Alw21I | GWGCWC | 4 cut(s) | 107, 360, 580, 657 |
| Alw26I | GTCTC | 8 cut(s) | 143, 834, 1061, 1248, 1432, 1679, 2089, 2154 |
| AlwI | GGATC | 5 cut(s) | 48, 1643, 1997, 2114, 2132 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 1466, 1640, 2799 |
| Ama87I | CYCGRG | 3 cut(s) | 1618, 2156, 2519 |
| AoxI | GGCC | 7 cut(s) | 318, 1478, 1724, 1958, 1966, 2213, 2634 |
| ApeKI | GCWGC | 6 cut(s) | 817, 873, 1304, 1460, 1570, 2696 |
| ApoI | RAATTY | 5 cut(s) | 348, 1388, 1665, 2382, 2605 |
| AsiGI | ACCGGT | 1 cut(s) | 2771 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 554, 2660 |
| Asp718I | GGTACC | 2 cut(s) | 1888, 2031 |
| AspA2I | CCTAGG | 1 cut(s) | 2186 |
| AspLEI | GCGC | 1 cut(s) | 2537 |
| AspS9I | GGNCC | 3 cut(s) | 518, 1395, 1967 |
| AsuC2I | CCSGG | 3 cut(s) | 1826, 2520, 2521 |
| AsuHPI | GGTGA | 6 cut(s) | 1196, 1639, 1807, 2079, 2215, 2344 |
| AsuII | TTCGAA | 1 cut(s) | 1762 |
| AsuNHI | GCTAGC | 1 cut(s) | 1586 |
| AvaI | CYCGRG | 3 cut(s) | 1618, 2156, 2519 |
| AvaII | GGWCC | 2 cut(s) | 518, 1395 |
| AvrII | CCTAGG | 1 cut(s) | 2186 |
| BanI | GGYRCC | 2 cut(s) | 1888, 2031 |
| BanII | GRGCYC | 4 cut(s) | 191, 657, 1537, 2527 |
| BbsI | GAAGAC | 2 cut(s) | 422, 2293 |
| Bbv12I | GWGCWC | 4 cut(s) | 107, 360, 580, 657 |
| BbvI | GCAGC | 6 cut(s) | 804, 885, 1291, 1472, 1557, 2708 |
| BccI | CCATC | 4 cut(s) | 203, 341, 481, 2656 |
| BceAI | ACGGC | 3 cut(s) | 1409, 1739, 1767 |
| BciT130I | CCWGG | 5 cut(s) | 804, 2395, 2489, 2616, 2794 |
| BciVI | GTATCC | 1 cut(s) | 1222 |
| BclI | TGATCA | 3 cut(s) | 405, 1602, 2761 |
| BcnI | CCSGG | 3 cut(s) | 1826, 2520, 2521 |
| BcoDI | GTCTC | 8 cut(s) | 143, 834, 1061, 1248, 1432, 1679, 2089, 2154 |
| BfaI | CTAG | 4 cut(s) | 114, 1587, 2144, 2187 |
| BfmI | CTRYAG | 3 cut(s) | 818, 925, 1038 |
| BfoI | RGCGCY | 1 cut(s) | 2538 |
| BfuAI | ACCTGC | 1 cut(s) | 994 |
| BfuI | GTATCC | 1 cut(s) | 1222 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 898 |
| BglII | AGATCT | 2 cut(s) | 1096, 1512 |
| BisI | GCNGC | 6 cut(s) | 818, 874, 1305, 1461, 1571, 2697 |
| BlnI | CCTAGG | 1 cut(s) | 2186 |
| BlpI | GCTNAGC | 2 cut(s) | 480, 579 |
| BlsI | GCNGC | 6 cut(s) | 819, 875, 1306, 1462, 1572, 2698 |
| BmcAI | AGTACT | 1 cut(s) | 731 |
| Bme1390I | CCNGG | 8 cut(s) | 804, 1826, 2395, 2489, 2520, 2521, 2616, 2794 |
| Bme18I | GGWCC | 2 cut(s) | 518, 1395 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 533 |
| BmeT110I | CYCGRG | 3 cut(s) | 1618, 2156, 2519 |
| BmgT120I | GGNCC | 3 cut(s) | 518, 1395, 1967 |
| BmiI | GGNNCC | 7 cut(s) | 520, 743, 1890, 2033, 2225, 2517, 2704 |
| BmrFI | CCNGG | 8 cut(s) | 804, 1826, 2395, 2489, 2520, 2521, 2616, 2794 |
| BmrI | ACTGGG | 1 cut(s) | 2369 |
| BmsI | GCATC | 7 cut(s) | 385, 1318, 1321, 1447, 1855, 2350, 2720 |
| BmtI | GCTAGC | 1 cut(s) | 1590 |
| BmuI | ACTGGG | 1 cut(s) | 2369 |
| BpiI | GAAGAC | 2 cut(s) | 422, 2293 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2150, 2182 |
| BpmI | CTGGAG | 3 cut(s) | 125, 1452, 2721 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 480, 579 |
| Bpu14I | TTCGAA | 1 cut(s) | 1762 |
| BpuEI | CTTGAG | 3 cut(s) | 1457, 1964, 2623 |
| BpuMI | CCSGG | 3 cut(s) | 1826, 2520, 2521 |
| Bsa29I | ATCGAT | 1 cut(s) | 198 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1802, 2647 |
| BsaJI | CCNNGG | 7 cut(s) | 84, 803, 1721, 2186, 2519, 2520, 2538 |
| BsaWI | WCCGGW | 1 cut(s) | 2771 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 625, 1026, 1624, 1824, 1825, 1842, 2316, 2712 |
| Bse118I | RCCGGY | 3 cut(s) | 1088, 2671, 2771 |
| Bse1I | ACTGG | 6 cut(s) | 142, 1154, 1288, 1961, 2275, 2375 |
| Bse3DI | GCAATG | 6 cut(s) | 254, 498, 1314, 1419, 1772, 2321 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1802, 2647 |
| BseBI | CCWGG | 5 cut(s) | 804, 2395, 2489, 2616, 2794 |
| BseCI | ATCGAT | 1 cut(s) | 198 |
| BseDI | CCNNGG | 7 cut(s) | 84, 803, 1721, 2186, 2519, 2520, 2538 |
| BseGI | GGATG | 5 cut(s) | 1312, 1888, 2251, 2725, 2735 |
| BseJI | GATNNNNATC | 2 cut(s) | 1802, 2647 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 625, 1026, 1624, 1824, 1825, 1842, 2316, 2712 |
| BseMI | GCAATG | 6 cut(s) | 254, 498, 1314, 1419, 1772, 2321 |
| BseMII | CTCAG | 8 cut(s) | 50, 110, 413, 494, 593, 1873, 2464, 2736 |
| BseNI | ACTGG | 6 cut(s) | 142, 1154, 1288, 1961, 2275, 2375 |
| BseRI | GAGGAG | 1 cut(s) | 1999 |
| BseXI | GCAGC | 6 cut(s) | 804, 885, 1291, 1472, 1557, 2708 |
| BsgI | GTGCAG | 3 cut(s) | 1022, 1085, 1452 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1089 |
| BshFI | GGCC | 7 cut(s) | 320, 1480, 1726, 1960, 1968, 2215, 2636 |
| BshNI | GGYRCC | 2 cut(s) | 1888, 2031 |
| BshTI | ACCGGT | 1 cut(s) | 2771 |
| BshVI | ATCGAT | 1 cut(s) | 198 |
| BsiEI | CGRYCG | 1 cut(s) | 1089 |
| BsiHKAI | GWGCWC | 4 cut(s) | 107, 360, 580, 657 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1618, 2156, 2519 |
| BsiSI | CCGG | 6 cut(s) | 1089, 1825, 2520, 2633, 2672, 2772 |
| BslFI | GGGAC | 3 cut(s) | 21, 504, 856 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 625, 1026, 1624, 1824, 1825, 1842, 2316, 2712 |
| BsmAI | GTCTC | 8 cut(s) | 143, 834, 1061, 1248, 1432, 1679, 2089, 2154 |
| BsmBI | CGTCTC | 2 cut(s) | 1248, 1432 |
| BsmFI | GGGAC | 3 cut(s) | 21, 504, 856 |
| BsmI | GAATGC | 3 cut(s) | 60, 1797, 1831 |
| BsnI | GGCC | 7 cut(s) | 320, 1480, 1726, 1960, 1968, 2215, 2636 |
| BsoBI | CYCGRG | 3 cut(s) | 1618, 2156, 2519 |
| Bsp119I | TTCGAA | 1 cut(s) | 1762 |
| Bsp1286I | GDGCHC | 7 cut(s) | 107, 191, 360, 580, 657, 1537, 2527 |
| Bsp1407I | TGTACA | 1 cut(s) | 777 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 480, 579 |
| BspACI | CCGC | 2 cut(s) | 1501, 1795 |
| BspANI | GGCC | 7 cut(s) | 320, 1480, 1726, 1960, 1968, 2215, 2636 |
| BspCNI | CTCAG | 8 cut(s) | 51, 109, 412, 493, 592, 1872, 2463, 2737 |
| BspDI | ATCGAT | 1 cut(s) | 198 |
| BspLI | GGNNCC | 7 cut(s) | 520, 743, 1890, 2033, 2225, 2517, 2704 |
| BspMAI | CTGCAG | 2 cut(s) | 822, 1042 |
| BspMI | ACCTGC | 1 cut(s) | 994 |
| BspOI | GCTAGC | 1 cut(s) | 1590 |
| BspPI | GGATC | 5 cut(s) | 48, 1643, 1997, 2114, 2132 |
| BspQI | GCTCTTC | 1 cut(s) | 95 |
| BspT104I | TTCGAA | 1 cut(s) | 1762 |
| BspT107I | GGYRCC | 2 cut(s) | 1888, 2031 |
| BsrBI | CCGCTC | 1 cut(s) | 1501 |
| BsrDI | GCAATG | 6 cut(s) | 254, 498, 1314, 1419, 1772, 2321 |
| BsrFI | RCCGGY | 3 cut(s) | 1088, 2671, 2771 |
| BsrGI | TGTACA | 1 cut(s) | 777 |
| BsrI | ACTGG | 6 cut(s) | 142, 1154, 1288, 1961, 2275, 2375 |
| BssAI | RCCGGY | 3 cut(s) | 1088, 2671, 2771 |
| BssECI | CCNNGG | 7 cut(s) | 84, 803, 1721, 2186, 2519, 2520, 2538 |
| BssT1I | CCWWGG | 3 cut(s) | 84, 2186, 2538 |
| Bst2UI | CCWGG | 5 cut(s) | 804, 2395, 2489, 2616, 2794 |
| Bst4CI | ACNGT | 6 cut(s) | 258, 541, 1190, 1225, 1402, 2235 |
| Bst6I | CTCTTC | 4 cut(s) | 95, 659, 1064, 2666 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 1443, 1466, 2789 |
| BstAUI | TGTACA | 1 cut(s) | 777 |
| BstBI | TTCGAA | 1 cut(s) | 1762 |
| BstC8I | GCNNGC | 6 cut(s) | 356, 1138, 1588, 2412, 2494, 2785 |
| BstDSI | CCRYGG | 1 cut(s) | 1721 |
| BstF5I | GGATG | 5 cut(s) | 1312, 1888, 2251, 2725, 2735 |
| BstH2I | RGCGCY | 1 cut(s) | 2538 |
| BstHHI | GCGC | 1 cut(s) | 2537 |
| BstMAI | GTCTC | 8 cut(s) | 143, 834, 1061, 1248, 1432, 1679, 2089, 2154 |
| BstMCI | CGRYCG | 1 cut(s) | 1089 |
| BstNI | CCWGG | 5 cut(s) | 804, 2395, 2489, 2616, 2794 |
| BstNSI | RCATGY | 1 cut(s) | 2671 |
| BstSCI | CCNGG | 8 cut(s) | 802, 1824, 2393, 2487, 2518, 2519, 2614, 2792 |
| BstSFI | CTRYAG | 3 cut(s) | 818, 925, 1038 |
| BstV1I | GCAGC | 6 cut(s) | 804, 885, 1291, 1472, 1557, 2708 |
| BstV2I | GAAGAC | 2 cut(s) | 422, 2293 |
| BstX2I | RGATCY | 2 cut(s) | 1096, 1512 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 281 |
| BstYI | RGATCY | 2 cut(s) | 1096, 1512 |
| Bsu15I | ATCGAT | 1 cut(s) | 198 |
| BsuI | GTATCC | 1 cut(s) | 1222 |
| BsuRI | GGCC | 7 cut(s) | 320, 1480, 1726, 1960, 1968, 2215, 2636 |
| BsuTUI | ATCGAT | 1 cut(s) | 198 |
| BtgI | CCRYGG | 1 cut(s) | 1721 |
| BtsCI | GGATG | 5 cut(s) | 1312, 1888, 2251, 2725, 2735 |
| BtsI | GCAGTG | 3 cut(s) | 1304, 1533, 1641 |
| BveI | ACCTGC | 1 cut(s) | 994 |
| Cac8I | GCNNGC | 6 cut(s) | 356, 1138, 1588, 2412, 2494, 2785 |
| CaiI | CAGNNNCTG | 3 cut(s) | 1466, 1640, 2799 |
| CfoI | GCGC | 1 cut(s) | 2537 |
| Cfr10I | RCCGGY | 3 cut(s) | 1088, 2671, 2771 |
| Cfr13I | GGNCC | 3 cut(s) | 518, 1395, 1967 |
| Cfr9I | CCCGGG | 1 cut(s) | 2519 |
| ClaI | ATCGAT | 1 cut(s) | 198 |
| CseI | GACGC | 1 cut(s) | 2403 |
| CspAI | ACCGGT | 1 cut(s) | 2771 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 605, 640 |
| DraIII | CACNNNGTG | 3 cut(s) | 575, 1299, 2789 |
| DriI | GACNNNNNGTC | 1 cut(s) | 533 |
| EaeI | YGGCCR | 1 cut(s) | 318 |
| Eam1104I | CTCTTC | 4 cut(s) | 95, 659, 1064, 2666 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 533 |
| EarI | CTCTTC | 4 cut(s) | 95, 659, 1064, 2666 |
| Ecl136II | GAGCTC | 1 cut(s) | 655 |
| Eco130I | CCWWGG | 3 cut(s) | 84, 2186, 2538 |
| Eco147I | AGGCCT | 1 cut(s) | 2215 |
| Eco24I | GRGCYC | 4 cut(s) | 191, 657, 1537, 2527 |
| Eco32I | GATATC | 3 cut(s) | 982, 1548, 2077 |
| Eco47I | GGWCC | 2 cut(s) | 518, 1395 |
| Eco53kI | GAGCTC | 1 cut(s) | 655 |
| Eco57I | CTGAAG | 1 cut(s) | 434 |
| Eco88I | CYCGRG | 3 cut(s) | 1618, 2156, 2519 |
| EcoICRI | GAGCTC | 1 cut(s) | 655 |
| EcoO109I | RGGNCCY | 1 cut(s) | 518 |
| EcoRII | CCWGG | 5 cut(s) | 802, 2393, 2487, 2614, 2792 |
| EcoRV | GATATC | 3 cut(s) | 982, 1548, 2077 |
| EcoT14I | CCWWGG | 3 cut(s) | 84, 2186, 2538 |
| EcoT22I | ATGCAT | 2 cut(s) | 1314, 1870 |
| EcoT38I | GRGCYC | 4 cut(s) | 191, 657, 1537, 2527 |
| ErhI | CCWWGG | 3 cut(s) | 84, 2186, 2538 |
| Esp3I | CGTCTC | 2 cut(s) | 1248, 1432 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 756, 788, 1043, 1075, 1989, 2021 |
| FaqI | GGGAC | 3 cut(s) | 21, 504, 856 |
| FauNDI | CATATG | 2 cut(s) | 976, 2511 |
| FbaI | TGATCA | 3 cut(s) | 405, 1602, 2761 |
| FblI | GTMKAC | 2 cut(s) | 675, 2776 |
| Fnu4HI | GCNGC | 6 cut(s) | 818, 874, 1305, 1461, 1571, 2697 |
| FokI | GGATG | 5 cut(s) | 1299, 1895, 2238, 2732, 2742 |
| FriOI | GRGCYC | 4 cut(s) | 191, 657, 1537, 2527 |
| Fsp4HI | GCNGC | 6 cut(s) | 818, 874, 1305, 1461, 1571, 2697 |
| FspBI | CTAG | 4 cut(s) | 114, 1587, 2144, 2187 |
| GlaI | GCGC | 1 cut(s) | 2536 |
| GluI | GCNGC | 6 cut(s) | 818, 874, 1305, 1461, 1571, 2697 |
| GsuI | CTGGAG | 3 cut(s) | 125, 1452, 2721 |
| HaeII | RGCGCY | 1 cut(s) | 2538 |
| HaeIII | GGCC | 7 cut(s) | 320, 1480, 1726, 1960, 1968, 2215, 2636 |
| HapII | CCGG | 6 cut(s) | 1089, 1825, 2520, 2633, 2672, 2772 |
| HgaI | GACGC | 1 cut(s) | 2403 |
| HhaI | GCGC | 1 cut(s) | 2537 |
| Hin6I | GCGC | 1 cut(s) | 2535 |
| HinP1I | GCGC | 1 cut(s) | 2535 |
| HincII | GTYRAC | 2 cut(s) | 676, 1159 |
| HindII | GTYRAC | 2 cut(s) | 676, 1159 |
| HindIII | AAGCTT | 3 cut(s) | 700, 1413, 2494 |
| HinfI | GANTC | 8 cut(s) | 296, 643, 669, 689, 799, 954, 1609, 2464 |
| HpaII | CCGG | 6 cut(s) | 1089, 1825, 2520, 2633, 2672, 2772 |
| HphI | GGTGA | 6 cut(s) | 1196, 1639, 1807, 2079, 2215, 2344 |
| Hpy166II | GTNNAC | 9 cut(s) | 160, 676, 778, 1009, 1159, 1193, 1398, 2352, 2777 |
| Hpy188III | TCNNGA | 9 cut(s) | 293, 547, 559, 693, 989, 1431, 1606, 1981, 2640 |
| Hpy8I | GTNNAC | 9 cut(s) | 160, 676, 778, 1009, 1159, 1193, 1398, 2352, 2777 |
| Hpy99I | CGWCG | 1 cut(s) | 677 |
| HpyCH4III | ACNGT | 6 cut(s) | 258, 541, 1190, 1225, 1402, 2235 |
| HspAI | GCGC | 1 cut(s) | 2535 |
| KpnI | GGTACC | 2 cut(s) | 1892, 2035 |
| Ksp22I | TGATCA | 3 cut(s) | 405, 1602, 2761 |
| LguI | GCTCTTC | 1 cut(s) | 95 |
| LmnI | GCTCC | 3 cut(s) | 600, 2294, 2702 |
| Lsp1109I | GCAGC | 6 cut(s) | 804, 885, 1291, 1472, 1557, 2708 |
| LweI | GCATC | 7 cut(s) | 385, 1318, 1321, 1447, 1855, 2350, 2720 |
| MaeI | CTAG | 4 cut(s) | 114, 1587, 2144, 2187 |
| MaeIII | GTNAC | 5 cut(s) | 121, 284, 535, 1184, 2277 |
| MbiI | CCGCTC | 1 cut(s) | 1501 |
| MflI | RGATCY | 2 cut(s) | 1096, 1512 |
| MhlI | GDGCHC | 7 cut(s) | 107, 191, 360, 580, 657, 1537, 2527 |
| MlyI | GAGTC | 3 cut(s) | 652, 948, 2473 |
| MmeI | TCCRAC | 5 cut(s) | 52, 352, 420, 622, 1384 |
| Mph1103I | ATGCAT | 2 cut(s) | 1314, 1870 |
| MroXI | GAANNNNTTC | 2 cut(s) | 554, 2660 |
| MseI | TTAA | 7 cut(s) | 1050, 1362, 1509, 1851, 2241, 2682, 2690 |
| MslI | CAYNNNNRTG | 2 cut(s) | 247, 1474 |
| MspA1I | CMGCKG | 2 cut(s) | 484, 2699 |
| MspI | CCGG | 6 cut(s) | 1089, 1825, 2520, 2633, 2672, 2772 |
| MspR9I | CCNGG | 8 cut(s) | 804, 1826, 2395, 2489, 2520, 2521, 2616, 2794 |
| Mva1269I | GAATGC | 3 cut(s) | 60, 1797, 1831 |
| MvaI | CCWGG | 5 cut(s) | 804, 2395, 2489, 2616, 2794 |
| NciI | CCSGG | 3 cut(s) | 1826, 2520, 2521 |
| NdeI | CATATG | 2 cut(s) | 976, 2511 |
| NheI | GCTAGC | 1 cut(s) | 1586 |
| NlaIV | GGNNCC | 7 cut(s) | 520, 743, 1890, 2033, 2225, 2517, 2704 |
| NmuCI | GTSAC | 4 cut(s) | 121, 284, 535, 1184 |
| NsiI | ATGCAT | 2 cut(s) | 1314, 1870 |
| NspI | RCATGY | 1 cut(s) | 2671 |
| NspV | TTCGAA | 1 cut(s) | 1762 |
| OliI | CACNNNNGTG | 1 cut(s) | 1474 |
| PaqCI | CACCTGC | 1 cut(s) | 994 |
| PceI | AGGCCT | 1 cut(s) | 2215 |
| PciSI | GCTCTTC | 1 cut(s) | 95 |
| PctI | GAATGC | 3 cut(s) | 60, 1797, 1831 |
| PdmI | GAANNNNTTC | 2 cut(s) | 554, 2660 |
| PfeI | GAWTC | 5 cut(s) | 296, 669, 689, 799, 1609 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1026, 2316 |
| PfoI | TCCNGGA | 1 cut(s) | 2393 |
| PinAI | ACCGGT | 1 cut(s) | 2771 |
| PkrI | GCNGC | 6 cut(s) | 819, 875, 1306, 1462, 1572, 2698 |
| PleI | GAGTC | 3 cut(s) | 651, 948, 2472 |
| PpsI | GAGTC | 3 cut(s) | 651, 948, 2472 |
| PpuMI | RGGWCCY | 1 cut(s) | 518 |
| Psp124BI | GAGCTC | 1 cut(s) | 657 |
| Psp5II | RGGWCCY | 1 cut(s) | 518 |
| Psp6I | CCWGG | 5 cut(s) | 802, 2393, 2487, 2614, 2792 |
| PspGI | CCWGG | 5 cut(s) | 802, 2393, 2487, 2614, 2792 |
| PspN4I | GGNNCC | 7 cut(s) | 520, 743, 1890, 2033, 2225, 2517, 2704 |
| PspPI | GGNCC | 3 cut(s) | 518, 1395, 1967 |
| PspPPI | RGGWCCY | 1 cut(s) | 518 |
| PstI | CTGCAG | 2 cut(s) | 822, 1042 |
| PstNI | CAGNNNCTG | 3 cut(s) | 1466, 1640, 2799 |
| PsuI | RGATCY | 2 cut(s) | 1096, 1512 |
| PvuII | CAGCTG | 2 cut(s) | 484, 2699 |
| RseI | CAYNNNNRTG | 2 cut(s) | 247, 1474 |
| SacI | GAGCTC | 1 cut(s) | 657 |
| SalI | GTCGAC | 1 cut(s) | 674 |
| SapI | GCTCTTC | 1 cut(s) | 95 |
| SaqAI | TTAA | 7 cut(s) | 1050, 1362, 1509, 1851, 2241, 2682, 2690 |
| SatI | GCNGC | 6 cut(s) | 818, 874, 1305, 1461, 1571, 2697 |
| Sau96I | GGNCC | 3 cut(s) | 518, 1395, 1967 |
| ScaI | AGTACT | 1 cut(s) | 731 |
| SchI | GAGTC | 3 cut(s) | 652, 948, 2473 |
| ScrFI | CCNGG | 8 cut(s) | 804, 1826, 2395, 2489, 2520, 2521, 2616, 2794 |
| SduI | GDGCHC | 7 cut(s) | 107, 191, 360, 580, 657, 1537, 2527 |
| SfaNI | GCATC | 7 cut(s) | 385, 1318, 1321, 1447, 1855, 2350, 2720 |
| SfcI | CTRYAG | 3 cut(s) | 818, 925, 1038 |
| SfuI | TTCGAA | 1 cut(s) | 1762 |
| SinI | GGWCC | 2 cut(s) | 518, 1395 |
| SmaI | CCCGGG | 1 cut(s) | 2521 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 247, 1474 |
| SmlI | CTYRAG | 3 cut(s) | 1472, 1979, 2638 |
| SmoI | CTYRAG | 3 cut(s) | 1472, 1979, 2638 |
| SseBI | AGGCCT | 1 cut(s) | 2215 |
| SsiI | CCGC | 2 cut(s) | 1501, 1795 |
| SspMI | CTAG | 4 cut(s) | 114, 1587, 2144, 2187 |
| SstI | GAGCTC | 1 cut(s) | 657 |
| StuI | AGGCCT | 1 cut(s) | 2215 |
| StyD4I | CCNGG | 8 cut(s) | 802, 1824, 2393, 2487, 2518, 2519, 2614, 2792 |
| StyI | CCWWGG | 3 cut(s) | 84, 2186, 2538 |
| TaaI | ACNGT | 6 cut(s) | 258, 541, 1190, 1225, 1402, 2235 |
| TatI | WGTACW | 5 cut(s) | 258, 729, 777, 852, 1125 |
| TfiI | GAWTC | 5 cut(s) | 296, 669, 689, 799, 1609 |
| Tru1I | TTAA | 7 cut(s) | 1050, 1362, 1509, 1851, 2241, 2682, 2690 |
| Tru9I | TTAA | 7 cut(s) | 1050, 1362, 1509, 1851, 2241, 2682, 2690 |
| TseFI | GTSAC | 4 cut(s) | 121, 284, 535, 1184 |
| TseI | GCWGC | 6 cut(s) | 817, 873, 1304, 1460, 1570, 2696 |
| Tsp45I | GTSAC | 4 cut(s) | 121, 284, 535, 1184 |
| TspMI | CCCGGG | 1 cut(s) | 2519 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1026, 2316 |
| VpaK11BI | GGWCC | 2 cut(s) | 518, 1395 |
| XapI | RAATTY | 5 cut(s) | 348, 1388, 1665, 2382, 2605 |
| XceI | RCATGY | 1 cut(s) | 2671 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 222 |
| XmaI | CCCGGG | 1 cut(s) | 2519 |
| XmaJI | CCTAGG | 1 cut(s) | 2186 |
| XmiI | GTMKAC | 2 cut(s) | 675, 2776 |
| XmnI | GAANNNNTTC | 2 cut(s) | 554, 2660 |
| XspI | CTAG | 4 cut(s) | 114, 1587, 2144, 2187 |
| ZrmI | AGTACT | 1 cut(s) | 731 |
| Zsp2I | ATGCAT | 2 cut(s) | 1314, 1870 |