Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0014157

Physical Location & Seq

Genomic Coordinates Forward (+)

16543149 .. 16546403

Gene Structure 3255 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1148600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 663 bp

ATGGCGATACTCTACGCCGTGGTGGCTCGAGGCACGGTGGTGTTGGCTGAGTTCAGCGCGGTTACAGGGAACACAGGGGCCGTGGCTCGGCGGATCTGCGAAAAGCTTCCGTCGGAGGCCGATGCTAGGGTTTGCTTCTCTCAAGATCGATACATCTTCCACATACTCAGAGCCGATGGCCTCACTTTCCTCTGTATGGCCAACGACACCTTCGGCAGTGCAATACCCTTTTCGTACTTGGAGGATATTCATATGAGGTTCATGAAAAATTATGGCAGAGTTGCCCCTTATGCTCCGGCTTATGCGATGAACGACGAATTTTCGAGGGTGTTGCATCAACAAATGGAGTTTTTCTCCAGTAATCCTAGCGCCGATACTCTCAACCGTGTGAGAGGCGAAGTTGGTGAGATACGAACTGTAATGGTGGACAACATAGAAAAGATATTGGATAGGGGTGACCGTATCGATCTTCTTGTTGACAAAACTGCAACTATGCAAGATGGTGCATTTCACTTCAAGAAGCAGTCTAAACGCCTTCGTCGAGCTCTGTGGATGAAAAATGCCAAGCTCTTGGCCCTGTTGACATGCTTGATTGTCGTGCTTCTGTACATCATAATAGCTGCTTGTTGTGGAGGCATCACACTACCTTCATGCAGATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000149 GO:0003674 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005794 GO:0006810 GO:0006887 GO:0006906 GO:0006950 GO:0006970 GO:0006996 GO:0008150 GO:0009628 GO:0009651 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0031201 GO:0032940 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0046903 GO:0048284 GO:0050896 GO:0051179 GO:0051234 GO:0061024 GO:0061025 GO:0071840 GO:0090174 GO:0098796

KEGG Pathways

Metabolic & Signaling

ko04130 map04130

Pfam Domains

Protein Families

Longin Synaptobrevin

Protein Analysis

221

Amino Acids

24.64

Weight (kDa)

8.86

Isoelectric Point (pI)

47.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Longin	PF13774	29 - 108	4e-21	Regulated-SNARE-like domain
Synaptobrevin	PF00957	127 - 206	3.3e-27	Synaptobrevin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014157)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22360
fragaria_vesca	FvH4_3g25480
malus_domestica	MD03G1148600.v1.1
prunus_persica	Prupe.6G134600_v2.0.a1 Prupe.6G134600_v2.0.a1 Prupe.6G134600_v2.0.a1
pyrus_communis	pycom03g10290
rosa_chinensis	RchiOBHm_Chr5g0046561
rosa_laevigata	RLG00000034418
rosa_multiflora	Rmu_sc0003878.1_g000037
rosa_roxburghii	Rroxscaffold_1G00034840
rosa_rugosa	Rorug05G0229600
rosa_samantha	Rh5AG309900 Rh5BG318300 Rh5CG344000 Rh5DG328600
rosa_wichuraiana	Rw5G028910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	59
AciI	CCGC	2 cut(s)	59, 91
AclWI	GGATC	1 cut(s)	101
AcoI	YGGCCR	1 cut(s)	198
AcsI	RAATTY	1 cut(s)	317
AfaI	GTAC	2 cut(s)	236, 608
AfiI	CCNNNNNNNGG	3 cut(s)	87, 126, 196
AgsI	TTSAA	1 cut(s)	517
AjuI	GAANNNNNNNTTGG	2 cut(s)	194, 226
AleI	CACNNNNGTG	1 cut(s)	38
AluBI	AGCT	4 cut(s)	106, 545, 568, 620
AluI	AGCT	4 cut(s)	106, 545, 568, 620
Alw21I	GWGCWC	1 cut(s)	547
AlwI	GGATC	1 cut(s)	101
Ama87I	CYCGRG	1 cut(s)	27
AoxI	GGCC	5 cut(s)	78, 117, 178, 198, 573
ApeKI	GCWGC	1 cut(s)	620
ApoI	RAATTY	1 cut(s)	317
Asp700I	GAANNNNTTC	1 cut(s)	105
AspLEI	GCGC	2 cut(s)	59, 371
AspS9I	GGNCC	2 cut(s)	78, 574
AsuHPI	GGTGA	2 cut(s)	416, 467
AvaI	CYCGRG	1 cut(s)	27
BalI	TGGCCA	1 cut(s)	200
BanII	GRGCYC	1 cut(s)	547
Bbv12I	GWGCWC	1 cut(s)	547
BbvI	GCAGC	1 cut(s)	607
BccI	CCATC	2 cut(s)	170, 494
BceAI	ACGGC	2 cut(s)	2, 65
BcgI	CGANNNNNNTGC	2 cut(s)	313, 347
BfaI	CTAG	2 cut(s)	126, 366
BfoI	RGCGCY	1 cut(s)	372
BglI	GCCNNNNNGGC	1 cut(s)	23
BglII	AGATCT	1 cut(s)	656
BisI	GCNGC	1 cut(s)	621
BlsI	GCNGC	1 cut(s)	622
BmeT110I	CYCGRG	1 cut(s)	27
BmgT120I	GGNCC	2 cut(s)	78, 574
BmiI	GGNNCC	1 cut(s)	79
BmsI	GCATC	3 cut(s)	112, 343, 645
BplI	GAGNNNNNCTC	2 cut(s)	338, 370
BpmI	CTGGAG	1 cut(s)	340
BpuEI	CTTGAG	1 cut(s)	126
Bsa29I	ATCGAT	2 cut(s)	148, 465
BsaJI	CCNNGG	2 cut(s)	18, 81
Bsc4I	CCNNNNNNNGG	3 cut(s)	87, 126, 196
Bse1I	ACTGG	1 cut(s)	357
BseCI	ATCGAT	2 cut(s)	148, 465
BseDI	CCNNGG	2 cut(s)	18, 81
BseGI	GGATG	1 cut(s)	558
BseLI	CCNNNNNNNGG	3 cut(s)	87, 126, 196
BseMII	CTCAG	2 cut(s)	39, 181
BseNI	ACTGG	1 cut(s)	357
BseXI	GCAGC	1 cut(s)	607
Bsh1236I	CGCG	1 cut(s)	59
BshFI	GGCC	5 cut(s)	80, 119, 180, 200, 575
BshVI	ATCGAT	2 cut(s)	148, 465
BsiHKAI	GWGCWC	1 cut(s)	547
BsiHKCI	CYCGRG	1 cut(s)	27
BsiSI	CCGG	1 cut(s)	296
BslI	CCNNNNNNNGG	3 cut(s)	87, 126, 196
BsnI	GGCC	5 cut(s)	80, 119, 180, 200, 575
BsoBI	CYCGRG	1 cut(s)	27
Bsp1286I	GDGCHC	1 cut(s)	547
Bsp1407I	TGTACA	1 cut(s)	606
Bsp143I	GATC	4 cut(s)	93, 145, 466, 656
BspACI	CCGC	2 cut(s)	59, 91
BspANI	GGCC	5 cut(s)	80, 119, 180, 200, 575
BspCNI	CTCAG	2 cut(s)	40, 180
BspDI	ATCGAT	2 cut(s)	148, 465
BspFNI	CGCG	1 cut(s)	59
BspHI	TCATGA	1 cut(s)	261
BspLI	GGNNCC	1 cut(s)	79
BspPI	GGATC	1 cut(s)	101
BsrGI	TGTACA	1 cut(s)	606
BsrI	ACTGG	1 cut(s)	357
BssECI	CCNNGG	2 cut(s)	18, 81
BssMI	GATC	4 cut(s)	93, 145, 466, 656
Bst4CI	ACNGT	4 cut(s)	37, 386, 418, 461
BstAUI	TGTACA	1 cut(s)	606
BstDEI	CTNAG	2 cut(s)	48, 167
BstDSI	CCRYGG	2 cut(s)	18, 81
BstEII	GGTNACC	1 cut(s)	455
BstF5I	GGATG	1 cut(s)	558
BstFNI	CGCG	1 cut(s)	59
BstH2I	RGCGCY	1 cut(s)	372
BstHHI	GCGC	2 cut(s)	59, 371
BstKTI	GATC	4 cut(s)	96, 148, 469, 659
BstMBI	GATC	4 cut(s)	93, 145, 466, 656
BstMWI	GCNNNNNNNGC	2 cut(s)	23, 290
BstNSI	RCATGY	1 cut(s)	588
BstPI	GGTNACC	1 cut(s)	455
BstUI	CGCG	1 cut(s)	59
BstV1I	GCAGC	1 cut(s)	607
BstX2I	RGATCY	2 cut(s)	93, 656
BstXI	CCANNNNNNTGG	1 cut(s)	571
BstYI	RGATCY	2 cut(s)	93, 656
Bsu15I	ATCGAT	2 cut(s)	148, 465
BsuRI	GGCC	5 cut(s)	80, 119, 180, 200, 575
BsuTUI	ATCGAT	2 cut(s)	148, 465
BtgI	CCRYGG	2 cut(s)	18, 81
BtgZI	GCGATG	1 cut(s)	320
BtsCI	GGATG	1 cut(s)	558
BtsI	GCAGTG	1 cut(s)	223
BtsIMutI	CAGTG	1 cut(s)	223
CciI	TCATGA	1 cut(s)	261
CfoI	GCGC	2 cut(s)	59, 371
Cfr13I	GGNCC	2 cut(s)	78, 574
ClaI	ATCGAT	2 cut(s)	148, 465
Csp6I	GTAC	2 cut(s)	235, 607
CviAII	CATG	3 cut(s)	262, 585, 651
CviQI	GTAC	2 cut(s)	235, 607
DdeI	CTNAG	2 cut(s)	48, 167
DpnI	GATC	4 cut(s)	95, 147, 468, 658
DpnII	GATC	4 cut(s)	93, 145, 466, 656
EaeI	YGGCCR	1 cut(s)	198
EciI	GGCGGA	1 cut(s)	106
Ecl136II	GAGCTC	1 cut(s)	545
Eco24I	GRGCYC	1 cut(s)	547
Eco53kI	GAGCTC	1 cut(s)	545
Eco88I	CYCGRG	1 cut(s)	27
Eco91I	GGTNACC	1 cut(s)	455
EcoICRI	GAGCTC	1 cut(s)	545
EcoO65I	GGTNACC	1 cut(s)	455
EcoT38I	GRGCYC	1 cut(s)	547
FaeI	CATG	3 cut(s)	265, 588, 654
FatI	CATG	3 cut(s)	261, 584, 650
FauNDI	CATATG	1 cut(s)	252
Fnu4HI	GCNGC	1 cut(s)	621
FokI	GGATG	1 cut(s)	565
FriOI	GRGCYC	1 cut(s)	547
Fsp4HI	GCNGC	1 cut(s)	621
FspBI	CTAG	2 cut(s)	126, 366
GlaI	GCGC	2 cut(s)	58, 370
GluI	GCNGC	1 cut(s)	621
GsuI	CTGGAG	1 cut(s)	340
HaeII	RGCGCY	1 cut(s)	372
HaeIII	GGCC	5 cut(s)	80, 119, 180, 200, 575
HapII	CCGG	1 cut(s)	296
HhaI	GCGC	2 cut(s)	59, 371
Hin1II	CATG	3 cut(s)	265, 588, 654
Hin6I	GCGC	2 cut(s)	57, 369
HinP1I	GCGC	2 cut(s)	57, 369
HincII	GTYRAC	2 cut(s)	478, 582
HindII	GTYRAC	2 cut(s)	478, 582
HindIII	AAGCTT	1 cut(s)	104
HpaII	CCGG	1 cut(s)	296
HphI	GGTGA	2 cut(s)	416, 467
Hpy166II	GTNNAC	3 cut(s)	427, 478, 582
Hpy188I	TCNGA	2 cut(s)	115, 170
Hpy188III	TCNNGA	4 cut(s)	143, 262, 517, 660
Hpy8I	GTNNAC	3 cut(s)	427, 478, 582
Hpy99I	CGWCG	3 cut(s)	115, 317, 543
HpyAV	CCTTC	3 cut(s)	220, 545, 657
HpyCH4III	ACNGT	4 cut(s)	37, 386, 418, 461
HpyCH4V	TGCA	6 cut(s)	221, 334, 488, 496, 506, 654
HpyF10VI	GCNNNNNNNGC	2 cut(s)	23, 290
HpyF3I	CTNAG	2 cut(s)	48, 167
Hsp92II	CATG	3 cut(s)	265, 588, 654
HspAI	GCGC	2 cut(s)	57, 369
Kzo9I	GATC	4 cut(s)	93, 145, 466, 656
LmnI	GCTCC	1 cut(s)	298
LpnPI	CCDG	5 cut(s)	51, 60, 309, 370, 590
Lsp1109I	GCAGC	1 cut(s)	607
LweI	GCATC	3 cut(s)	112, 343, 645
MaeI	CTAG	2 cut(s)	126, 366
MaeIII	GTNAC	2 cut(s)	61, 455
MalI	GATC	4 cut(s)	95, 147, 468, 658
MboI	GATC	4 cut(s)	93, 145, 466, 656
MboII	GAAGA	2 cut(s)	148, 461
MflI	RGATCY	2 cut(s)	93, 656
MhlI	GDGCHC	1 cut(s)	547
MlsI	TGGCCA	1 cut(s)	200
MluCI	AATT	2 cut(s)	268, 317
MluNI	TGGCCA	1 cut(s)	200
MmeI	TCCRAC	1 cut(s)	93
MnlI	CCTC	9 cut(s)	23, 109, 191, 200, 235, 249, 318, 386, 626
Mox20I	TGGCCA	1 cut(s)	200
MroXI	GAANNNNTTC	1 cut(s)	105
MscI	TGGCCA	1 cut(s)	200
MslI	CAYNNNNRTG	1 cut(s)	38
Msp20I	TGGCCA	1 cut(s)	200
MspI	CCGG	1 cut(s)	296
MvnI	CGCG	1 cut(s)	59
MwoI	GCNNNNNNNGC	2 cut(s)	23, 290
NdeI	CATATG	1 cut(s)	252
NdeII	GATC	4 cut(s)	93, 145, 466, 656
NlaIII	CATG	3 cut(s)	265, 588, 654
NlaIV	GGNNCC	1 cut(s)	79
NmeAIII	GCCGAG	1 cut(s)	67
NmuCI	GTSAC	1 cut(s)	455
NspI	RCATGY	1 cut(s)	588
OliI	CACNNNNGTG	1 cut(s)	38
PaeR7I	CTCGAG	1 cut(s)	27
PagI	TCATGA	1 cut(s)	261
PcsI	WCGNNNNNNNCGW	1 cut(s)	538
PdmI	GAANNNNTTC	1 cut(s)	105
PkrI	GCNGC	1 cut(s)	622
Psp124BI	GAGCTC	1 cut(s)	547
PspEI	GGTNACC	1 cut(s)	455
PspN4I	GGNNCC	1 cut(s)	79
PspPI	GGNCC	2 cut(s)	78, 574
PspXI	VCTCGAGB	1 cut(s)	27
PsuI	RGATCY	2 cut(s)	93, 656
RsaI	GTAC	2 cut(s)	236, 608
RsaNI	GTAC	2 cut(s)	235, 607
RseI	CAYNNNNRTG	1 cut(s)	38
SacI	GAGCTC	1 cut(s)	547
SatI	GCNGC	1 cut(s)	621
Sau3AI	GATC	4 cut(s)	93, 145, 466, 656
Sau96I	GGNCC	2 cut(s)	78, 574
SduI	GDGCHC	1 cut(s)	547
SetI	ASST	7 cut(s)	108, 212, 260, 547, 570, 622, 649
SfaNI	GCATC	3 cut(s)	112, 343, 645
Sfr274I	CTCGAG	1 cut(s)	27
SlaI	CTCGAG	1 cut(s)	27
SmiMI	CAYNNNNRTG	1 cut(s)	38
SmlI	CTYRAG	2 cut(s)	27, 141
SmoI	CTYRAG	2 cut(s)	27, 141
Sse9I	AATT	2 cut(s)	268, 317
SsiI	CCGC	2 cut(s)	59, 91
SspMI	CTAG	2 cut(s)	126, 366
SstI	GAGCTC	1 cut(s)	547
TaaI	ACNGT	4 cut(s)	37, 386, 418, 461
TaqI	TCGA	5 cut(s)	28, 148, 323, 465, 541
TasI	AATT	2 cut(s)	268, 317
TatI	WGTACW	1 cut(s)	606
TscAI	CASTG	1 cut(s)	223
TseFI	GTSAC	1 cut(s)	455
TseI	GCWGC	1 cut(s)	620
Tsp45I	GTSAC	1 cut(s)	455
TspDTI	ATGAA	6 cut(s)	239, 250, 278, 323, 569, 639
TspGWI	ACGGA	1 cut(s)	99
TspRI	CASTG	1 cut(s)	223
XapI	RAATTY	1 cut(s)	317
XceI	RCATGY	1 cut(s)	588
XhoI	CTCGAG	1 cut(s)	27
XmnI	GAANNNNTTC	1 cut(s)	105
XspI	CTAG	2 cut(s)	126, 366

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.