pycom03g10290

vesicle-associated membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0014157
Physical Location & Seq
Forward (+)
9437611 .. 9440537
2927 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g10290.3

Sequence Viewer

Length: 663 bp
ATGGCGATACTCTACGCCGTGGTGGCTCGAGGCACGGTGGTGTTGGCCGAGTTCAGCGCGGCTACAGGGAACACAGGGGCCGTGGCTCGGCGGATCTGCGAAAAGCTTCCGTCGGAGGCCGATGCTAGGGTTTGCTTCTCTCAAGATCGATACATCTTCCACATACTCAGAGCCGATGGCCTCACTTTCCTCTGTATGGCCAACGACACCTTCGGCAGGAGAATACCCTTTTCGTACTTGGAGGATATTCATATGAGGTTCATGAAAAATTATGGCAGAGTTGCCCCTTATGCTCCGGCTTATGCGATGAACGACGAATTTTCGAGGGTGTTGCATCAACAAATGGAGTTTTTCTCCAGTAATCCTAGCGCCGATACTCTCAACCGTGTGAGAGGCGAAGTTGGTGAGATACGAACTGTAATGGTGGACAACATAGAAAAGATATTGGATAGGGGTGACCGTATTGATCTTCTTGTTGACAAAACTGCAACTATGCAAGATGGTGCATTTCACTTCAAGAAGCAGTCTAAACGCCTTCGTCGAGCTCTGTGGATGAAAAATGCCAAGCTCTTGGCCCTGTTGACATGCTTGATTGTCGTGCTTCTGTACATCATAATAGCTGCTTGTTGTGGAGGCATCACACTACCTTCATGCAGATCTTGA

Protein Analysis

221

Amino Acids

24.77

Weight (kDa)

9.14

Isoelectric Point (pI)

50.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22360
fragaria_vesca FvH4_3g25480
malus_domestica MD03G1148600.v1.1
prunus_persica Prupe.6G134600_v2.0.a1 Prupe.6G134600_v2.0.a1 Prupe.6G134600_v2.0.a1
pyrus_communis pycom03g10290
rosa_chinensis RchiOBHm_Chr5g0046561
rosa_laevigata RLG00000034418
rosa_multiflora Rmu_sc0003878.1_g000037
rosa_roxburghii Rroxscaffold_1G00034840
rosa_rugosa Rorug05G0229600
rosa_samantha Rh5AG309900 Rh5BG318300 Rh5CG344000 Rh5DG328600
rosa_wichuraiana Rw5G028910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 59
AciI CCGC 2 cut(s) 59, 91
AclWI GGATC 1 cut(s) 101
AcoI YGGCCR 2 cut(s) 45, 198
AcsI RAATTY 1 cut(s) 317
AfaI GTAC 2 cut(s) 236, 608
AfiI CCNNNNNNNGG 4 cut(s) 87, 126, 196, 216
AgsI TTSAA 1 cut(s) 517
AjuI GAANNNNNNNTTGG 2 cut(s) 194, 226
AleI CACNNNNGTG 1 cut(s) 38
AluBI AGCT 4 cut(s) 106, 545, 568, 620
AluI AGCT 4 cut(s) 106, 545, 568, 620
Alw21I GWGCWC 1 cut(s) 547
AlwI GGATC 1 cut(s) 101
Ama87I CYCGRG 1 cut(s) 27
AoxI GGCC 6 cut(s) 45, 78, 117, 178, 198, 573
ApeKI GCWGC 1 cut(s) 620
ApoI RAATTY 1 cut(s) 317
Asp700I GAANNNNTTC 1 cut(s) 105
AspLEI GCGC 2 cut(s) 59, 371
AspS9I GGNCC 2 cut(s) 78, 574
AsuHPI GGTGA 2 cut(s) 416, 467
AvaI CYCGRG 1 cut(s) 27
BalI TGGCCA 1 cut(s) 200
BanII GRGCYC 1 cut(s) 547
Bbv12I GWGCWC 1 cut(s) 547
BbvI GCAGC 1 cut(s) 607
BccI CCATC 2 cut(s) 170, 494
BceAI ACGGC 2 cut(s) 2, 65
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BfaI CTAG 2 cut(s) 126, 366
BfmI CTRYAG 1 cut(s) 63
BfoI RGCGCY 1 cut(s) 372
BglI GCCNNNNNGGC 1 cut(s) 23
BglII AGATCT 1 cut(s) 656
BisI GCNGC 2 cut(s) 60, 621
BlsI GCNGC 2 cut(s) 61, 622
BmeT110I CYCGRG 1 cut(s) 27
BmgT120I GGNCC 2 cut(s) 78, 574
BmiI GGNNCC 1 cut(s) 79
BmsI GCATC 3 cut(s) 112, 343, 645
BplI GAGNNNNNCTC 2 cut(s) 338, 370
BpmI CTGGAG 1 cut(s) 340
BpuEI CTTGAG 1 cut(s) 126
Bsa29I ATCGAT 1 cut(s) 148
BsaJI CCNNGG 2 cut(s) 18, 81
Bsc4I CCNNNNNNNGG 4 cut(s) 87, 126, 196, 216
Bse1I ACTGG 1 cut(s) 357
BseCI ATCGAT 1 cut(s) 148
BseDI CCNNGG 2 cut(s) 18, 81
BseGI GGATG 1 cut(s) 558
BseLI CCNNNNNNNGG 4 cut(s) 87, 126, 196, 216
BseMII CTCAG 1 cut(s) 181
BseNI ACTGG 1 cut(s) 357
BseXI GCAGC 1 cut(s) 607
Bsh1236I CGCG 1 cut(s) 59
BshFI GGCC 6 cut(s) 47, 80, 119, 180, 200, 575
BshVI ATCGAT 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 547
BsiHKCI CYCGRG 1 cut(s) 27
BsiSI CCGG 1 cut(s) 296
BslI CCNNNNNNNGG 4 cut(s) 87, 126, 196, 216
BsnI GGCC 6 cut(s) 47, 80, 119, 180, 200, 575
BsoBI CYCGRG 1 cut(s) 27
Bsp1286I GDGCHC 1 cut(s) 547
Bsp1407I TGTACA 1 cut(s) 606
Bsp143I GATC 4 cut(s) 93, 145, 466, 656
BspACI CCGC 2 cut(s) 59, 91
BspANI GGCC 6 cut(s) 47, 80, 119, 180, 200, 575
BspCNI CTCAG 1 cut(s) 180
BspDI ATCGAT 1 cut(s) 148
BspFNI CGCG 1 cut(s) 59
BspHI TCATGA 1 cut(s) 261
BspLI GGNNCC 1 cut(s) 79
BspPI GGATC 1 cut(s) 101
BsrGI TGTACA 1 cut(s) 606
BsrI ACTGG 1 cut(s) 357
BssECI CCNNGG 2 cut(s) 18, 81
BssMI GATC 4 cut(s) 93, 145, 466, 656
Bst4CI ACNGT 4 cut(s) 37, 386, 418, 461
BstAUI TGTACA 1 cut(s) 606
BstDEI CTNAG 1 cut(s) 167
BstDSI CCRYGG 2 cut(s) 18, 81
BstEII GGTNACC 1 cut(s) 455
BstENI CCTNNNNNAGG 1 cut(s) 214
BstF5I GGATG 1 cut(s) 558
BstFNI CGCG 1 cut(s) 59
BstH2I RGCGCY 1 cut(s) 372
BstHHI GCGC 2 cut(s) 59, 371
BstKTI GATC 4 cut(s) 96, 148, 469, 659
BstMBI GATC 4 cut(s) 93, 145, 466, 656
BstMWI GCNNNNNNNGC 2 cut(s) 23, 290
BstNSI RCATGY 1 cut(s) 588
BstPI GGTNACC 1 cut(s) 455
BstSFI CTRYAG 1 cut(s) 63
BstUI CGCG 1 cut(s) 59
BstV1I GCAGC 1 cut(s) 607
BstX2I RGATCY 2 cut(s) 93, 656
BstXI CCANNNNNNTGG 1 cut(s) 571
BstYI RGATCY 2 cut(s) 93, 656
Bsu15I ATCGAT 1 cut(s) 148
BsuRI GGCC 6 cut(s) 47, 80, 119, 180, 200, 575
BsuTUI ATCGAT 1 cut(s) 148
BtgI CCRYGG 2 cut(s) 18, 81
BtgZI GCGATG 1 cut(s) 320
BtsCI GGATG 1 cut(s) 558
CciI TCATGA 1 cut(s) 261
CfoI GCGC 2 cut(s) 59, 371
Cfr13I GGNCC 2 cut(s) 78, 574
ClaI ATCGAT 1 cut(s) 148
Csp6I GTAC 2 cut(s) 235, 607
CviAII CATG 3 cut(s) 262, 585, 651
CviQI GTAC 2 cut(s) 235, 607
DdeI CTNAG 1 cut(s) 167
DpnI GATC 4 cut(s) 95, 147, 468, 658
DpnII GATC 4 cut(s) 93, 145, 466, 656
EaeI YGGCCR 2 cut(s) 45, 198
EciI GGCGGA 1 cut(s) 106
Ecl136II GAGCTC 1 cut(s) 545
Eco24I GRGCYC 1 cut(s) 547
Eco53kI GAGCTC 1 cut(s) 545
Eco88I CYCGRG 1 cut(s) 27
Eco91I GGTNACC 1 cut(s) 455
EcoICRI GAGCTC 1 cut(s) 545
EcoNI CCTNNNNNAGG 1 cut(s) 214
EcoO65I GGTNACC 1 cut(s) 455
EcoT38I GRGCYC 1 cut(s) 547
FaeI CATG 3 cut(s) 265, 588, 654
FatI CATG 3 cut(s) 261, 584, 650
FauNDI CATATG 1 cut(s) 252
Fnu4HI GCNGC 2 cut(s) 60, 621
FokI GGATG 1 cut(s) 565
FriOI GRGCYC 1 cut(s) 547
Fsp4HI GCNGC 2 cut(s) 60, 621
FspBI CTAG 2 cut(s) 126, 366
GlaI GCGC 2 cut(s) 58, 370
GluI GCNGC 2 cut(s) 60, 621
GsuI CTGGAG 1 cut(s) 340
HaeII RGCGCY 1 cut(s) 372
HaeIII GGCC 6 cut(s) 47, 80, 119, 180, 200, 575
HapII CCGG 1 cut(s) 296
HhaI GCGC 2 cut(s) 59, 371
Hin1II CATG 3 cut(s) 265, 588, 654
Hin6I GCGC 2 cut(s) 57, 369
HinP1I GCGC 2 cut(s) 57, 369
HincII GTYRAC 2 cut(s) 478, 582
HindII GTYRAC 2 cut(s) 478, 582
HindIII AAGCTT 1 cut(s) 104
HpaII CCGG 1 cut(s) 296
HphI GGTGA 2 cut(s) 416, 467
Hpy166II GTNNAC 3 cut(s) 427, 478, 582
Hpy188I TCNGA 2 cut(s) 115, 170
Hpy188III TCNNGA 4 cut(s) 143, 262, 517, 660
Hpy8I GTNNAC 3 cut(s) 427, 478, 582
Hpy99I CGWCG 3 cut(s) 115, 317, 543
HpyAV CCTTC 3 cut(s) 220, 545, 657
HpyCH4III ACNGT 4 cut(s) 37, 386, 418, 461
HpyCH4V TGCA 5 cut(s) 334, 488, 496, 506, 654
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 290
HpyF3I CTNAG 1 cut(s) 167
Hsp92II CATG 3 cut(s) 265, 588, 654
HspAI GCGC 2 cut(s) 57, 369
Kzo9I GATC 4 cut(s) 93, 145, 466, 656
LmnI GCTCC 1 cut(s) 298
LpnPI CCDG 6 cut(s) 51, 60, 202, 309, 370, 590
Lsp1109I GCAGC 1 cut(s) 607
LweI GCATC 3 cut(s) 112, 343, 645
MaeI CTAG 2 cut(s) 126, 366
MaeIII GTNAC 1 cut(s) 455
MalI GATC 4 cut(s) 95, 147, 468, 658
MboI GATC 4 cut(s) 93, 145, 466, 656
MboII GAAGA 2 cut(s) 148, 461
MflI RGATCY 2 cut(s) 93, 656
MhlI GDGCHC 1 cut(s) 547
MlsI TGGCCA 1 cut(s) 200
MluCI AATT 2 cut(s) 268, 317
MluNI TGGCCA 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 93
MnlI CCTC 9 cut(s) 23, 109, 191, 200, 235, 249, 318, 386, 626
Mox20I TGGCCA 1 cut(s) 200
MroXI GAANNNNTTC 1 cut(s) 105
MscI TGGCCA 1 cut(s) 200
MslI CAYNNNNRTG 1 cut(s) 38
Msp20I TGGCCA 1 cut(s) 200
MspI CCGG 1 cut(s) 296
MvnI CGCG 1 cut(s) 59
MwoI GCNNNNNNNGC 2 cut(s) 23, 290
NdeI CATATG 1 cut(s) 252
NdeII GATC 4 cut(s) 93, 145, 466, 656
NlaIII CATG 3 cut(s) 265, 588, 654
NlaIV GGNNCC 1 cut(s) 79
NmeAIII GCCGAG 2 cut(s) 67, 73
NmuCI GTSAC 1 cut(s) 455
NspI RCATGY 1 cut(s) 588
OliI CACNNNNGTG 1 cut(s) 38
PaeR7I CTCGAG 1 cut(s) 27
PagI TCATGA 1 cut(s) 261
PcsI WCGNNNNNNNCGW 1 cut(s) 538
PdmI GAANNNNTTC 1 cut(s) 105
PkrI GCNGC 2 cut(s) 61, 622
Psp124BI GAGCTC 1 cut(s) 547
PspEI GGTNACC 1 cut(s) 455
PspN4I GGNNCC 1 cut(s) 79
PspPI GGNCC 2 cut(s) 78, 574
PspXI VCTCGAGB 1 cut(s) 27
PsuI RGATCY 2 cut(s) 93, 656
RsaI GTAC 2 cut(s) 236, 608
RsaNI GTAC 2 cut(s) 235, 607
RseI CAYNNNNRTG 1 cut(s) 38
SacI GAGCTC 1 cut(s) 547
SatI GCNGC 2 cut(s) 60, 621
Sau3AI GATC 4 cut(s) 93, 145, 466, 656
Sau96I GGNCC 2 cut(s) 78, 574
SduI GDGCHC 1 cut(s) 547
SetI ASST 7 cut(s) 108, 212, 260, 547, 570, 622, 649
SfaNI GCATC 3 cut(s) 112, 343, 645
SfcI CTRYAG 1 cut(s) 63
Sfr274I CTCGAG 1 cut(s) 27
SlaI CTCGAG 1 cut(s) 27
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 2 cut(s) 27, 141
SmoI CTYRAG 2 cut(s) 27, 141
Sse9I AATT 2 cut(s) 268, 317
SsiI CCGC 2 cut(s) 59, 91
SspMI CTAG 2 cut(s) 126, 366
SstI GAGCTC 1 cut(s) 547
TaaI ACNGT 4 cut(s) 37, 386, 418, 461
TaqI TCGA 4 cut(s) 28, 148, 323, 541
TasI AATT 2 cut(s) 268, 317
TatI WGTACW 1 cut(s) 606
TauI GCSGC 1 cut(s) 62
TseFI GTSAC 1 cut(s) 455
TseI GCWGC 1 cut(s) 620
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 6 cut(s) 239, 250, 278, 323, 569, 639
TspGWI ACGGA 1 cut(s) 99
XagI CCTNNNNNAGG 1 cut(s) 214
XapI RAATTY 1 cut(s) 317
XceI RCATGY 1 cut(s) 588
XhoI CTCGAG 1 cut(s) 27
XmnI GAANNNNTTC 1 cut(s) 105
XspI CTAG 2 cut(s) 126, 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.