MD03G1169200.v1.1

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014247
Physical Location & Seq
Reverse (-)
22891346 .. 22896557
5212 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1169200.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGGCCATGGCTTCCAGACTCTCACCTACTTCGATTCTTAATCTTCCAATCCCAGCTAGAAATCTCCCAAACCACCCTCACAGAATGCCTCTTTTTTCTATTCCACCACCTCTGGATCTCCATGCAAATTCCTCTATCAATTCAACAACCATTTCGCGCTCTAAAGGACGATCATCGCCACCGGCACTTGGTGCTGTCCAAGAAGAAGTCGTCCAGTCCCCAAGTTCAGAGCGCACCGGAAATTCAGAAGCTTCCCCTTCTTCCTCTTCCAAGCTCGTTCTCGTCATTGGCGGCACCGGCGGCGTTGGTCAGCTGGTGGTGGCTTCGTTGCTCGACCGGAATATCAAGTCCCGACTATTGTTGCGTGACCCCGAGAAAGCCACTACATTATTTGGTGAACACGATGAAGAAACATTGCAGATATTCAAAGGGGACACTCGGAATCCAAAGGATCTGGATCCATCTATTTTTGAGGGAGTCACCCATGTGATTTGCTGCTCGGGAACTACTGCCTTTCCTTCAAAGCGATGGGAAGGCGATAATACACCAGAAAGAGTTGATTGGGAGGGTGTGAGAAATCTTATATCTGCACTGCCTTCATCGCTGAAGAGAATTGTTCTTGTATCATCAGTAGGCGTAACCAAGTCAAATGAACTGCCATGGAGCATTATGAACCTTTTTGGCGTTCTCAAATATAAAAAGATGGGGGAGGATATGCTTCGTAATTCTGGCCTACCATTTACAATAATCAGACCTGGTAGATTGACAGATGGACCTTACACATCATATGATCTTAATACATTGCTCAAAGCTACAGCTGGCAAACGGCGTGCAGTCCTATTAGGTCAAGGAGATAAGCTGGTTGGAGAGGTTAGCAGGCATGTCGTTGCTGAAGCTTGCATACAGGCGTTGGACATAGAATGTACTGAAGGACAAATTTTTGAAGTCAACTCGGTCGAGGGAGAAGGCCCAGGAAGTGATCAGCAAAAGTGGCGGGAATTGTTCAAAGCTGCCCGAGCTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

342

Amino Acids

36.97

Weight (kDa)

8.31

Isoelectric Point (pI)

54.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 90 - 168 1.5e-07 NmrA-like family
Epimerase PF01370 93 - 214 8.9e-08 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 94 - 225 1.4e-06 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 97 - 305 2.2e-29 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014247)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31530 AT4G31530
fragaria_vesca FvH4_3g28150
malus_domestica MD03G1169200.v1.1
prunus_persica Prupe.4G258400_v2.0.a1 Prupe.4G258400_v2.0.a1
pyrus_communis pycom03g12540
rosa_chinensis RchiOBHm_Chr5g0050831
rosa_laevigata RLG00000034742
rosa_multiflora Rmu_sc0007394.1_g000008
rosa_roxburghii Rroxscaffold_1G00029880
rosa_rugosa Rorug05G0264500.1
rosa_samantha Rh5AG336500 Rh5BG346000 Rh5CG373700 Rh5DG359000
rosa_wichuraiana Rw5G031730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 293
AccII CGCG 1 cut(s) 157
AciI CCGC 3 cut(s) 291, 300, 994
AclWI GGATC 4 cut(s) 123, 452, 459, 465
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 127, 241, 936
AcuI CTGAAG 3 cut(s) 626, 912, 948
AdeI CACNNNGTG 1 cut(s) 191
AfaI GTAC 1 cut(s) 925
AfiI CCNNNNNNNGG 1 cut(s) 188
AgsI TTSAA 5 cut(s) 144, 427, 522, 944, 1006
AjnI CCWGG 2 cut(s) 754, 970
AleI CACNNNNGTG 1 cut(s) 485
Alw21I GWGCWC 1 cut(s) 1021
AlwI GGATC 4 cut(s) 123, 452, 459, 465
Ama87I CYCGRG 3 cut(s) 371, 499, 1014
AoxI GGCC 3 cut(s) 3, 730, 967
ApeKI GCWGC 2 cut(s) 495, 1010
ApoI RAATTY 3 cut(s) 127, 241, 936
AspLEI GCGC 2 cut(s) 159, 234
AspS9I GGNCC 2 cut(s) 773, 968
AsuHPI GGTGA 3 cut(s) 15, 407, 472
AvaI CYCGRG 3 cut(s) 371, 499, 1014
AvaII GGWCC 1 cut(s) 773
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 457
BanI GGYRCC 1 cut(s) 293
BanII GRGCYC 1 cut(s) 1021
BarI GAAGNNNNNNTAC 2 cut(s) 885, 917
Bbv12I GWGCWC 1 cut(s) 1021
BbvI GCAGC 2 cut(s) 482, 997
BccI CCATC 4 cut(s) 469, 522, 697, 764
BceAI ACGGC 1 cut(s) 842
BcgI CGANNNNNNTGC 2 cut(s) 865, 899
BciT130I CCWGG 2 cut(s) 756, 972
BclI TGATCA 1 cut(s) 979
BfaI CTAG 1 cut(s) 57
BfmI CTRYAG 1 cut(s) 813
BisI GCNGC 4 cut(s) 292, 301, 496, 1011
BlsI GCNGC 4 cut(s) 293, 302, 497, 1012
Bme1390I CCNGG 2 cut(s) 756, 972
Bme18I GGWCC 1 cut(s) 773
BmeT110I CYCGRG 3 cut(s) 371, 499, 1014
BmgT120I GGNCC 2 cut(s) 773, 968
BmiI GGNNCC 2 cut(s) 295, 459
BmrFI CCNGG 2 cut(s) 756, 972
BsaBI GATNNNNATC 1 cut(s) 456
BsaJI CCNNGG 3 cut(s) 6, 659, 970
BsaWI WCCGGW 2 cut(s) 236, 336
Bsc4I CCNNNNNNNGG 1 cut(s) 188
Bse118I RCCGGY 2 cut(s) 181, 296
Bse1I ACTGG 1 cut(s) 214
Bse3DI GCAATG 2 cut(s) 413, 800
Bse8I GATNNNNATC 1 cut(s) 456
BseBI CCWGG 2 cut(s) 756, 972
BseDI CCNNGG 3 cut(s) 6, 659, 970
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 1 cut(s) 188
BseMI GCAATG 2 cut(s) 413, 800
BseNI ACTGG 1 cut(s) 214
BseXI GCAGC 2 cut(s) 482, 997
BseYI CCCAGC 1 cut(s) 52
BsgI GTGCAG 2 cut(s) 573, 852
Bsh1236I CGCG 1 cut(s) 157
Bsh1285I CGRYCG 2 cut(s) 337, 957
BshFI GGCC 3 cut(s) 5, 732, 969
BshNI GGYRCC 1 cut(s) 293
BsiEI CGRYCG 2 cut(s) 337, 957
BsiHKAI GWGCWC 1 cut(s) 1021
BsiHKCI CYCGRG 3 cut(s) 371, 499, 1014
BsiSI CCGG 4 cut(s) 182, 237, 297, 337
BslFI GGGAC 3 cut(s) 202, 334, 446
BslI CCNNNNNNNGG 1 cut(s) 188
BsmFI GGGAC 3 cut(s) 202, 334, 446
BsmI GAATGC 1 cut(s) 90
BsnI GGCC 3 cut(s) 5, 732, 969
BsoBI CYCGRG 3 cut(s) 371, 499, 1014
Bsp1286I GDGCHC 1 cut(s) 1021
Bsp143I GATC 6 cut(s) 115, 170, 451, 457, 790, 979
Bsp19I CCATGG 2 cut(s) 6, 659
BspACI CCGC 3 cut(s) 291, 300, 994
BspANI GGCC 3 cut(s) 5, 732, 969
BspFNI CGCG 1 cut(s) 157
BspLI GGNNCC 2 cut(s) 295, 459
BspPI GGATC 4 cut(s) 123, 452, 459, 465
BspT107I GGYRCC 1 cut(s) 293
BsrDI GCAATG 2 cut(s) 413, 800
BsrFI RCCGGY 2 cut(s) 181, 296
BsrI ACTGG 1 cut(s) 214
BssAI RCCGGY 2 cut(s) 181, 296
BssECI CCNNGG 3 cut(s) 6, 659, 970
BssMI GATC 6 cut(s) 115, 170, 451, 457, 790, 979
BssT1I CCWWGG 2 cut(s) 6, 659
Bst2UI CCWGG 2 cut(s) 756, 972
Bst6I CTCTTC 2 cut(s) 271, 602
BstAPI GCANNNNNTGC 1 cut(s) 191
BstC8I GCNNGC 4 cut(s) 820, 831, 878, 898
BstDEI CTNAG 1 cut(s) 1020
BstDSI CCRYGG 2 cut(s) 6, 659
BstFNI CGCG 1 cut(s) 157
BstHHI GCGC 2 cut(s) 159, 234
BstKTI GATC 6 cut(s) 118, 173, 454, 460, 793, 982
BstMBI GATC 6 cut(s) 115, 170, 451, 457, 790, 979
BstMCI CGRYCG 2 cut(s) 337, 957
BstMWI GCNNNNNNNGC 6 cut(s) 191, 297, 300, 601, 991, 1016
BstNI CCWGG 2 cut(s) 756, 972
BstNSI RCATGY 1 cut(s) 884
BstSCI CCNGG 2 cut(s) 754, 970
BstSFI CTRYAG 1 cut(s) 813
BstUI CGCG 1 cut(s) 157
BstV1I GCAGC 2 cut(s) 482, 997
BstX2I RGATCY 3 cut(s) 115, 451, 457
BstYI RGATCY 3 cut(s) 115, 451, 457
BsuRI GGCC 3 cut(s) 5, 732, 969
BtgI CCRYGG 2 cut(s) 6, 659
BtgZI GCGATG 3 cut(s) 159, 541, 585
BtsI GCAGTG 1 cut(s) 590
BtsIMutI CAGTG 1 cut(s) 590
Cac8I GCNNGC 4 cut(s) 820, 831, 878, 898
CfoI GCGC 2 cut(s) 159, 234
Cfr10I RCCGGY 2 cut(s) 181, 296
Cfr13I GGNCC 2 cut(s) 773, 968
CsiI ACCWGGT 1 cut(s) 754
Csp6I GTAC 1 cut(s) 924
CviAII CATG 5 cut(s) 7, 122, 485, 660, 881
CviQI GTAC 1 cut(s) 924
DdeI CTNAG 1 cut(s) 1020
DpnI GATC 6 cut(s) 117, 172, 453, 459, 792, 981
DpnII GATC 6 cut(s) 115, 170, 451, 457, 790, 979
DraIII CACNNNGTG 1 cut(s) 191
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 271, 602
EarI CTCTTC 2 cut(s) 271, 602
Ecl136II GAGCTC 1 cut(s) 1019
Eco130I CCWWGG 2 cut(s) 6, 659
Eco24I GRGCYC 1 cut(s) 1021
Eco47I GGWCC 1 cut(s) 773
Eco53kI GAGCTC 1 cut(s) 1019
Eco57I CTGAAG 3 cut(s) 626, 912, 948
Eco88I CYCGRG 3 cut(s) 371, 499, 1014
EcoICRI GAGCTC 1 cut(s) 1019
EcoRII CCWGG 2 cut(s) 754, 970
EcoT14I CCWWGG 2 cut(s) 6, 659
EcoT38I GRGCYC 1 cut(s) 1021
ErhI CCWWGG 2 cut(s) 6, 659
FaeI CATG 5 cut(s) 10, 125, 488, 663, 884
FaqI GGGAC 3 cut(s) 202, 334, 446
FatI CATG 5 cut(s) 6, 121, 484, 659, 880
FauI CCCGC 1 cut(s) 987
FauNDI CATATG 1 cut(s) 787
FbaI TGATCA 1 cut(s) 979
Fnu4HI GCNGC 4 cut(s) 292, 301, 496, 1011
FriOI GRGCYC 1 cut(s) 1021
Fsp4HI GCNGC 4 cut(s) 292, 301, 496, 1011
FspBI CTAG 1 cut(s) 57
GlaI GCGC 2 cut(s) 158, 233
GluI GCNGC 4 cut(s) 292, 301, 496, 1011
GsaI CCCAGC 1 cut(s) 56
HaeIII GGCC 3 cut(s) 5, 732, 969
HapII CCGG 4 cut(s) 182, 237, 297, 337
HhaI GCGC 2 cut(s) 159, 234
Hin1II CATG 5 cut(s) 10, 125, 488, 663, 884
Hin6I GCGC 2 cut(s) 157, 232
HinP1I GCGC 2 cut(s) 157, 232
HincII GTYRAC 1 cut(s) 949
HindII GTYRAC 1 cut(s) 949
HindIII AAGCTT 2 cut(s) 249, 894
HinfI GANTC 4 cut(s) 18, 34, 442, 477
HpaII CCGG 4 cut(s) 182, 237, 297, 337
HphI GGTGA 3 cut(s) 15, 407, 472
Hpy166II GTNNAC 2 cut(s) 398, 949
Hpy188I TCNGA 4 cut(s) 229, 247, 441, 752
Hpy188III TCNNGA 5 cut(s) 15, 113, 351, 455, 501
Hpy8I GTNNAC 2 cut(s) 398, 949
HpyAV CCTTC 6 cut(s) 267, 527, 528, 606, 923, 959
HpyCH4V TGCA 5 cut(s) 125, 418, 590, 833, 900
HpyF10VI GCNNNNNNNGC 6 cut(s) 191, 297, 300, 601, 991, 1016
HpyF3I CTNAG 1 cut(s) 1020
Hsp92II CATG 5 cut(s) 10, 125, 488, 663, 884
HspAI GCGC 2 cut(s) 157, 232
Ksp22I TGATCA 1 cut(s) 979
Kzo9I GATC 6 cut(s) 115, 170, 451, 457, 790, 979
LmnI GCTCC 1 cut(s) 663
Lsp1109I GCAGC 2 cut(s) 482, 997
MabI ACCWGGT 1 cut(s) 754
MaeI CTAG 1 cut(s) 57
MaeIII GTNAC 3 cut(s) 365, 478, 637
MalI GATC 6 cut(s) 117, 172, 453, 459, 792, 981
MboI GATC 6 cut(s) 115, 170, 451, 457, 790, 979
MboII GAAGA 6 cut(s) 35, 215, 252, 258, 419, 619
MflI RGATCY 3 cut(s) 115, 451, 457
MhlI GDGCHC 1 cut(s) 1021
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 127, 139, 241, 612, 724, 936, 998
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 12, 486
MmeI TCCRAC 2 cut(s) 844, 891
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 39, 795
MslI CAYNNNNRTG 1 cut(s) 485
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 313, 818
MspI CCGG 4 cut(s) 182, 237, 297, 337
MspR9I CCNGG 2 cut(s) 756, 972
Mva1269I GAATGC 1 cut(s) 90
MvaI CCWGG 2 cut(s) 756, 972
MvnI CGCG 1 cut(s) 157
MwoI GCNNNNNNNGC 6 cut(s) 191, 297, 300, 601, 991, 1016
NcoI CCATGG 2 cut(s) 6, 659
NdeI CATATG 1 cut(s) 787
NdeII GATC 6 cut(s) 115, 170, 451, 457, 790, 979
NlaIII CATG 5 cut(s) 10, 125, 488, 663, 884
NlaIV GGNNCC 2 cut(s) 295, 459
NmuCI GTSAC 2 cut(s) 365, 478
NspI RCATGY 1 cut(s) 884
OliI CACNNNNGTG 1 cut(s) 485
PctI GAATGC 1 cut(s) 90
PfeI GAWTC 2 cut(s) 34, 442
PkrI GCNGC 4 cut(s) 293, 302, 497, 1012
PleI GAGTC 2 cut(s) 12, 485
PpsI GAGTC 2 cut(s) 12, 485
Psp124BI GAGCTC 1 cut(s) 1021
Psp6I CCWGG 2 cut(s) 754, 970
PspFI CCCAGC 1 cut(s) 52
PspGI CCWGG 2 cut(s) 754, 970
PspN4I GGNNCC 2 cut(s) 295, 459
PspPI GGNCC 2 cut(s) 773, 968
PsuI RGATCY 3 cut(s) 115, 451, 457
PvuII CAGCTG 2 cut(s) 313, 818
RsaI GTAC 1 cut(s) 925
RsaNI GTAC 1 cut(s) 924
RseI CAYNNNNRTG 1 cut(s) 485
SacI GAGCTC 1 cut(s) 1021
SaqAI TTAA 2 cut(s) 39, 795
SatI GCNGC 4 cut(s) 292, 301, 496, 1011
Sau3AI GATC 6 cut(s) 115, 170, 451, 457, 790, 979
Sau96I GGNCC 2 cut(s) 773, 968
SchI GAGTC 2 cut(s) 12, 486
ScrFI CCNGG 2 cut(s) 756, 972
SduI GDGCHC 1 cut(s) 1021
SexAI ACCWGGT 1 cut(s) 754
SfcI CTRYAG 1 cut(s) 813
SgrAI CRCCGGYG 1 cut(s) 296
SinI GGWCC 1 cut(s) 773
SmiMI CAYNNNNRTG 1 cut(s) 485
Sse9I AATT 7 cut(s) 127, 139, 241, 612, 724, 936, 998
SsiI CCGC 3 cut(s) 291, 300, 994
SspMI CTAG 1 cut(s) 57
SstI GAGCTC 1 cut(s) 1021
StyD4I CCNGG 2 cut(s) 754, 970
StyI CCWWGG 2 cut(s) 6, 659
TaqI TCGA 3 cut(s) 32, 333, 957
TaqII GACCGA 1 cut(s) 943
TasI AATT 7 cut(s) 127, 139, 241, 612, 724, 936, 998
TatI WGTACW 1 cut(s) 923
TauI GCSGC 2 cut(s) 294, 303
TfiI GAWTC 2 cut(s) 34, 442
Tru1I TTAA 2 cut(s) 39, 795
Tru9I TTAA 2 cut(s) 39, 795
TscAI CASTG 1 cut(s) 597
TseFI GTSAC 2 cut(s) 365, 478
TseI GCWGC 2 cut(s) 495, 1010
Tsp45I GTSAC 2 cut(s) 365, 478
TspDTI ATGAA 4 cut(s) 420, 588, 666, 686
TspRI CASTG 1 cut(s) 597
VpaK11BI GGWCC 1 cut(s) 773
XapI RAATTY 3 cut(s) 127, 241, 936
XceI RCATGY 1 cut(s) 884
XspI CTAG 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.