Prupe.4G258400_v2.0.a1

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0014247
Physical Location & Seq
Forward (+)
18384306 .. 18389893
5588 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G258400.1

Sequence Viewer

Length: 1008 bp
ATGGCTTCCAGACTCTCACTCATTTCCATTCCCAATCTTCCAACCCCAGCTAGAACATTCCCAAAACACCCTCAACAAATACCTCTTTTTTCTCTTCCACCTCATAGTTTTCATGTAAATCCCTCCTTCAATTCGAGGACCATTTCGAGCTCTAATGGAGGATCATCGCCAGCACTTCATGCTGTCCAAGAGGAAGTGACCACCAGTTCAGATTCCACCAGTGATTCCAAAACTGCCCCTTCCTCCTCTTCCAAGGTCGTTCTCGTCATTGGAGGCACCGGCGGTGTTGGTCAGCTGGTAGTGGCTTCGTTGCTCAACCGGAATATCAAGGCCCGCCTATTATTGCGTGACCCTGAGAAAGCAACTACGTTGTTTGGTGAACAAGATGAAGAGAAATTGCAGGTCTTCAAAGGAGACACTCGGAATGCAGACGATCTAGATCCATCTATTTTTGAGGGAGTCACACATGTGATTTGCTGCACAGGAACTACGGCTTTTCCTTCGAAGCGGTGGGAAGGGGATAATACACCAGAAAGAGTTGATTGGGAGGGCGTGAGAAATCTTGTATCTGTACTGCCTTCTTCGCTGCAGAGAGTTGTTCTTGTATCATCAGTAGGTGTAACCAAGTTCAATGAACTGCCTTGGAGCATTATGAACCTTTTTGGTGTTCTCAAATATAAGAAGATGGGGGAGGATATTCTTCGTAATTCTGGTCTATCATTTACTATAATCAGACCTGGTAGATTGACAGATGGACCTTACACATCATATGATCTTAATACATTGCTGAAAGCTACAGCTGGCCAACGACGTGCAGTCCTAATAGGTCAAGGAGATAAACTAGTTGGAGAGGTTAGCAGACTTGTTGTTGCTGAAGCTTGCATACAGGCATTGGACATAGAATGTACTGAAGGAAAAACTTTTGAAGTTAACTCAGTTGAGGGAGAAGGTCCAGGAAGCGATCAACAAAAGTGGAGGGAATTATTCAAAGCTGCCCAAGCTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

336

Amino Acids

36.28

Weight (kDa)

6.55

Isoelectric Point (pI)

47.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014247)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31530 AT4G31530
fragaria_vesca FvH4_3g28150
malus_domestica MD03G1169200.v1.1
prunus_persica Prupe.4G258400_v2.0.a1 Prupe.4G258400_v2.0.a1
pyrus_communis pycom03g12540
rosa_chinensis RchiOBHm_Chr5g0050831
rosa_laevigata RLG00000034742
rosa_multiflora Rmu_sc0007394.1_g000008
rosa_roxburghii Rroxscaffold_1G00029880
rosa_rugosa Rorug05G0264500.1
rosa_samantha Rh5AG336500 Rh5BG346000 Rh5CG373700 Rh5DG359000
rosa_wichuraiana Rw5G031730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 391
AccB1I GGYRCC 1 cut(s) 275
AciI CCGC 3 cut(s) 282, 334, 508
AclWI GGATC 2 cut(s) 169, 434
AcoI YGGCCR 1 cut(s) 802
AcuI CTGAAG 2 cut(s) 894, 930
AfaI GTAC 2 cut(s) 573, 907
AflIII ACRYGT 1 cut(s) 466
AgsI TTSAA 5 cut(s) 130, 409, 631, 926, 988
AhdI GACNNNNNGTC 1 cut(s) 815
AhlI ACTAGT 1 cut(s) 841
AjiI CACGTC 1 cut(s) 812
AjnI CCWGG 2 cut(s) 736, 952
AleI CACNNNNGTG 1 cut(s) 467
AluBI AGCT 8 cut(s) 50, 150, 295, 794, 800, 878, 992, 1001
AluI AGCT 8 cut(s) 50, 150, 295, 794, 800, 878, 992, 1001
Alw21I GWGCWC 1 cut(s) 152
Alw26I GTCTC 1 cut(s) 408
AlwI GGATC 2 cut(s) 169, 434
AoxI GGCC 2 cut(s) 330, 802
ApeKI GCWGC 3 cut(s) 477, 586, 992
AspS9I GGNCC 4 cut(s) 138, 331, 755, 950
AsuHPI GGTGA 1 cut(s) 389
AsuII TTCGAA 1 cut(s) 503
AvaII GGWCC 3 cut(s) 138, 755, 950
BalI TGGCCA 1 cut(s) 804
BanI GGYRCC 1 cut(s) 275
BanII GRGCYC 1 cut(s) 152
BarI GAAGNNNNNNTAC 2 cut(s) 867, 899
BbsI GAAGAC 1 cut(s) 397
Bbv12I GWGCWC 1 cut(s) 152
BbvI GCAGC 3 cut(s) 464, 573, 979
BccI CCATC 3 cut(s) 451, 679, 746
BceAI ACGGC 1 cut(s) 507
BciT130I CCWGG 2 cut(s) 738, 954
BcoDI GTCTC 1 cut(s) 408
BcuI ACTAGT 1 cut(s) 841
BfaI CTAG 3 cut(s) 51, 437, 842
BfmI CTRYAG 2 cut(s) 587, 795
BfuAI ACCTGC 1 cut(s) 391
BisI GCNGC 3 cut(s) 478, 587, 993
BlsI GCNGC 3 cut(s) 479, 588, 994
Bme1390I CCNGG 2 cut(s) 738, 954
Bme18I GGWCC 3 cut(s) 138, 755, 950
BmeRI GACNNNNNGTC 1 cut(s) 815
BmgBI CACGTC 1 cut(s) 812
BmgT120I GGNCC 4 cut(s) 138, 331, 755, 950
BmiI GGNNCC 1 cut(s) 277
BmrFI CCNGG 2 cut(s) 738, 954
BpiI GAAGAC 1 cut(s) 397
Bpu14I TTCGAA 1 cut(s) 503
BsaBI GATNNNNATC 1 cut(s) 438
BsaJI CCNNGG 2 cut(s) 252, 641
BsaWI WCCGGW 1 cut(s) 318
Bse118I RCCGGY 1 cut(s) 278
Bse1I ACTGG 2 cut(s) 204, 219
Bse3DI GCAATG 1 cut(s) 782
Bse8I GATNNNNATC 1 cut(s) 438
BseBI CCWGG 2 cut(s) 738, 954
BseDI CCNNGG 2 cut(s) 252, 641
BseJI GATNNNNATC 1 cut(s) 438
BseMI GCAATG 1 cut(s) 782
BseMII CTCAG 2 cut(s) 345, 948
BseNI ACTGG 2 cut(s) 204, 219
BseRI GAGGAG 1 cut(s) 235
BseXI GCAGC 3 cut(s) 464, 573, 979
BseYI CCCAGC 1 cut(s) 46
BsgI GTGCAG 2 cut(s) 463, 834
BshFI GGCC 2 cut(s) 332, 804
BshNI GGYRCC 1 cut(s) 275
BsiHKAI GWGCWC 1 cut(s) 152
BsiSI CCGG 2 cut(s) 279, 319
BsmAI GTCTC 1 cut(s) 408
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 2 cut(s) 332, 804
Bsp119I TTCGAA 1 cut(s) 503
Bsp1286I GDGCHC 1 cut(s) 152
Bsp143I GATC 5 cut(s) 161, 433, 439, 772, 961
BspACI CCGC 3 cut(s) 282, 334, 508
BspANI GGCC 2 cut(s) 332, 804
BspCNI CTCAG 2 cut(s) 346, 947
BspLI GGNNCC 1 cut(s) 277
BspMAI CTGCAG 1 cut(s) 591
BspMI ACCTGC 1 cut(s) 391
BspPI GGATC 2 cut(s) 169, 434
BspT104I TTCGAA 1 cut(s) 503
BspT107I GGYRCC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 782
BsrFI RCCGGY 1 cut(s) 278
BsrI ACTGG 2 cut(s) 204, 219
BssAI RCCGGY 1 cut(s) 278
BssECI CCNNGG 2 cut(s) 252, 641
BssMI GATC 5 cut(s) 161, 433, 439, 772, 961
BssT1I CCWWGG 2 cut(s) 252, 641
Bst2UI CCWGG 2 cut(s) 738, 954
Bst6I CTCTTC 3 cut(s) 99, 253, 384
BstAPI GCANNNNNTGC 1 cut(s) 179
BstBI TTCGAA 1 cut(s) 503
BstC8I GCNNGC 4 cut(s) 171, 334, 802, 880
BstDEI CTNAG 2 cut(s) 354, 934
BstKTI GATC 5 cut(s) 164, 436, 442, 775, 964
BstMAI GTCTC 1 cut(s) 408
BstMBI GATC 5 cut(s) 161, 433, 439, 772, 961
BstMWI GCNNNNNNNGC 3 cut(s) 179, 583, 998
BstNI CCWGG 2 cut(s) 738, 954
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 2 cut(s) 736, 952
BstSFI CTRYAG 2 cut(s) 587, 795
BstV1I GCAGC 3 cut(s) 464, 573, 979
BstV2I GAAGAC 1 cut(s) 397
BstX2I RGATCY 1 cut(s) 439
BstYI RGATCY 1 cut(s) 439
BsuRI GGCC 2 cut(s) 332, 804
BtgZI GCGATG 1 cut(s) 150
BtrI CACGTC 1 cut(s) 812
BtsIMutI CAGTG 1 cut(s) 226
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 4 cut(s) 171, 334, 802, 880
Cfr10I RCCGGY 1 cut(s) 278
Cfr13I GGNCC 4 cut(s) 138, 331, 755, 950
CsiI ACCWGGT 1 cut(s) 736
Csp6I GTAC 2 cut(s) 572, 906
CspCI CAANNNNNGTGG 2 cut(s) 953, 988
CviAII CATG 3 cut(s) 113, 179, 467
CviQI GTAC 2 cut(s) 572, 906
DdeI CTNAG 2 cut(s) 354, 934
DpnI GATC 5 cut(s) 163, 435, 441, 774, 963
DpnII GATC 5 cut(s) 161, 433, 439, 772, 961
DriI GACNNNNNGTC 1 cut(s) 815
EaeI YGGCCR 1 cut(s) 802
Eam1104I CTCTTC 3 cut(s) 99, 253, 384
Eam1105I GACNNNNNGTC 1 cut(s) 815
EarI CTCTTC 3 cut(s) 99, 253, 384
Ecl136II GAGCTC 1 cut(s) 150
Eco130I CCWWGG 2 cut(s) 252, 641
Eco24I GRGCYC 1 cut(s) 152
Eco47I GGWCC 3 cut(s) 138, 755, 950
Eco53kI GAGCTC 1 cut(s) 150
Eco57I CTGAAG 2 cut(s) 894, 930
EcoICRI GAGCTC 1 cut(s) 150
EcoRII CCWGG 2 cut(s) 736, 952
EcoT14I CCWWGG 2 cut(s) 252, 641
EcoT38I GRGCYC 1 cut(s) 152
ErhI CCWWGG 2 cut(s) 252, 641
FaeI CATG 3 cut(s) 116, 182, 470
FatI CATG 3 cut(s) 112, 178, 466
FauI CCCGC 1 cut(s) 341
FauNDI CATATG 1 cut(s) 769
Fnu4HI GCNGC 3 cut(s) 478, 587, 993
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 3 cut(s) 478, 587, 993
FspBI CTAG 3 cut(s) 51, 437, 842
GluI GCNGC 3 cut(s) 478, 587, 993
GsaI CCCAGC 1 cut(s) 50
HaeIII GGCC 2 cut(s) 332, 804
HapII CCGG 2 cut(s) 279, 319
Hin1II CATG 3 cut(s) 116, 182, 470
HincII GTYRAC 1 cut(s) 931
HindII GTYRAC 1 cut(s) 931
HindIII AAGCTT 1 cut(s) 876
HinfI GANTC 4 cut(s) 12, 212, 224, 459
HpaI GTTAAC 1 cut(s) 931
HpaII CCGG 2 cut(s) 279, 319
HphI GGTGA 1 cut(s) 389
Hpy166II GTNNAC 2 cut(s) 380, 931
Hpy188I TCNGA 3 cut(s) 211, 423, 734
Hpy188III TCNNGA 2 cut(s) 9, 437
Hpy8I GTNNAC 2 cut(s) 380, 931
Hpy99I CGWCG 1 cut(s) 813
HpyAV CCTTC 7 cut(s) 136, 249, 509, 510, 588, 905, 941
HpyCH4IV ACGT 2 cut(s) 368, 811
HpyCH4V TGCA 6 cut(s) 400, 428, 480, 589, 815, 882
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 583, 998
HpyF3I CTNAG 2 cut(s) 354, 934
HpySE526I ACGT 2 cut(s) 368, 811
Hsp92II CATG 3 cut(s) 116, 182, 470
KspAI GTTAAC 1 cut(s) 931
Kzo9I GATC 5 cut(s) 161, 433, 439, 772, 961
LmnI GCTCC 1 cut(s) 645
Lsp1109I GCAGC 3 cut(s) 464, 573, 979
MabI ACCWGGT 1 cut(s) 736
MaeI CTAG 3 cut(s) 51, 437, 842
MaeII ACGT 2 cut(s) 368, 811
MaeIII GTNAC 4 cut(s) 196, 347, 460, 619
MalI GATC 5 cut(s) 163, 435, 441, 774, 963
MboI GATC 5 cut(s) 161, 433, 439, 772, 961
MboII GAAGA 8 cut(s) 29, 86, 240, 397, 401, 573, 692, 694
MflI RGATCY 1 cut(s) 439
MhlI GDGCHC 1 cut(s) 152
MlsI TGGCCA 1 cut(s) 804
MluCI AATT 4 cut(s) 130, 395, 706, 980
MluNI TGGCCA 1 cut(s) 804
MlyI GAGTC 2 cut(s) 6, 468
MmeI TCCRAC 2 cut(s) 65, 826
Mox20I TGGCCA 1 cut(s) 804
MscI TGGCCA 1 cut(s) 804
MseI TTAA 2 cut(s) 777, 930
MslI CAYNNNNRTG 1 cut(s) 467
Msp20I TGGCCA 1 cut(s) 804
MspA1I CMGCKG 2 cut(s) 295, 800
MspI CCGG 2 cut(s) 279, 319
MspR9I CCNGG 2 cut(s) 738, 954
Mva1269I GAATGC 1 cut(s) 430
MvaI CCWGG 2 cut(s) 738, 954
MwoI GCNNNNNNNGC 3 cut(s) 179, 583, 998
NdeI CATATG 1 cut(s) 769
NdeII GATC 5 cut(s) 161, 433, 439, 772, 961
NlaIII CATG 3 cut(s) 116, 182, 470
NlaIV GGNNCC 1 cut(s) 277
NmuCI GTSAC 3 cut(s) 196, 347, 460
NspI RCATGY 1 cut(s) 470
NspV TTCGAA 1 cut(s) 503
OliI CACNNNNGTG 1 cut(s) 467
PciI ACATGT 1 cut(s) 466
PctI GAATGC 1 cut(s) 430
PfeI GAWTC 2 cut(s) 212, 224
PfoI TCCNGGA 1 cut(s) 952
PkrI GCNGC 3 cut(s) 479, 588, 994
PleI GAGTC 2 cut(s) 6, 467
PpsI GAGTC 2 cut(s) 6, 467
PscI ACATGT 1 cut(s) 466
Psp124BI GAGCTC 1 cut(s) 152
Psp6I CCWGG 2 cut(s) 736, 952
PspFI CCCAGC 1 cut(s) 46
PspGI CCWGG 2 cut(s) 736, 952
PspN4I GGNNCC 1 cut(s) 277
PspPI GGNCC 4 cut(s) 138, 331, 755, 950
PstI CTGCAG 1 cut(s) 591
PsuI RGATCY 1 cut(s) 439
PvuII CAGCTG 2 cut(s) 295, 800
RsaI GTAC 2 cut(s) 573, 907
RsaNI GTAC 2 cut(s) 572, 906
RseI CAYNNNNRTG 1 cut(s) 467
SacI GAGCTC 1 cut(s) 152
SaqAI TTAA 2 cut(s) 777, 930
SatI GCNGC 3 cut(s) 478, 587, 993
Sau3AI GATC 5 cut(s) 161, 433, 439, 772, 961
Sau96I GGNCC 4 cut(s) 138, 331, 755, 950
SchI GAGTC 2 cut(s) 6, 468
ScrFI CCNGG 2 cut(s) 738, 954
SduI GDGCHC 1 cut(s) 152
SexAI ACCWGGT 1 cut(s) 736
SfcI CTRYAG 2 cut(s) 587, 795
SfuI TTCGAA 1 cut(s) 503
SgrAI CRCCGGYG 1 cut(s) 278
SinI GGWCC 3 cut(s) 138, 755, 950
SmiMI CAYNNNNRTG 1 cut(s) 467
SpeI ACTAGT 1 cut(s) 841
Sse9I AATT 4 cut(s) 130, 395, 706, 980
SsiI CCGC 3 cut(s) 282, 334, 508
SspMI CTAG 3 cut(s) 51, 437, 842
SstI GAGCTC 1 cut(s) 152
StyD4I CCNGG 2 cut(s) 736, 952
StyI CCWWGG 2 cut(s) 252, 641
TaiI ACGT 2 cut(s) 371, 814
TaqI TCGA 3 cut(s) 134, 146, 503
TasI AATT 4 cut(s) 130, 395, 706, 980
TatI WGTACW 2 cut(s) 571, 905
TfiI GAWTC 2 cut(s) 212, 224
Tru1I TTAA 2 cut(s) 777, 930
Tru9I TTAA 2 cut(s) 777, 930
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 3 cut(s) 196, 347, 460
TseI GCWGC 3 cut(s) 477, 586, 992
Tsp45I GTSAC 3 cut(s) 196, 347, 460
TspDTI ATGAA 5 cut(s) 101, 167, 402, 648, 668
TspRI CASTG 1 cut(s) 226
VpaK11BI GGWCC 3 cut(s) 138, 755, 950
XbaI TCTAGA 1 cut(s) 436
XceI RCATGY 1 cut(s) 470
XspI CTAG 3 cut(s) 51, 437, 842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.