RLG00000034742

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014247
Physical Location & Seq
Reverse (-)
53338206 .. 53342897
4692 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034742

Sequence Viewer

Length: 1254 bp
ATGGCTTTCAGACTCTCACTCACTTCCACTGCCAATTTTCAAATCTCAACCCCAAAACACCCTCGACAATTACCTCTTCCTCTTCTGGGTTTTCAGTTAAAATGTTGCTCCAATTCCATTTCGCGCTCAAAAGCGGGAGCAGTTCATGCTGTTCAACAGGAGCAGGAGGTTCTCCAGTCCCCAGCTTCAGATTCCAATTCTGTTCCTTCTTCCTCGTCCAAGCTCGTTCTCGTCGTCGGTGGTAGCGGCGGTGTTGGTCAGCTGGTAGTGGCTTCATTGCTGGATCGGAATATCAAGTCTCGCCTACTATTGCGTGACACTGAGAAAGCAACTGCATTATTTGGTGAACAAGACCAAGAAACATTGCAGGTATTCAAAGGGGACACTCGCAACCCAGAAGATCTAGATCCATCTATATTTGAGGGAGTTACACATGTGATTTGCTGCACTGGAACTACAGCCTTTCCCTCGAAGCGCTGGAGTGGGGATAATACACCAGAAAAAGTTGATTGGGAGGGTGTGAGAAATTTGGTATCTGCACTGCCTCGGTCACTGAAGAGAATTGTTCTTGTATCATCAGTTGGTGTAACCAAGTTCAATGAACTGCCATGGAGTGTTATGAACCTCTTCGGTGTTCTCAAGTATAAGAAGATGGGGGAAGACATTCTTCGTAATTCTGGTCTACCATTTACAATAATCAGGGCTGGTAGATTGACTGATGGACCTTACACATCATATGATCTTAATACATTGCTCAAAGCTACAGCTGGCCAACGACGTGCAGTCCTAATAGGTCAAGGAGATACACTAGTTGGAGAGGTTAGCAGACTTGTTGTTGCTGAAGCTTGCATACAGGCACTGGACATAGAATGCACTGAAGGAAAAATATTTGAAGTCAACTCAGTTGAGTTCCTTTTCGGCTTGTTCTCAAATAGCACGTTGCTTTTGCTGTTTTGTGTCAATGATTTGGAACACATTTGTTGTTGCGAAGTTTATGAGCCAACTCCTATTTGTGTTGGACTCTTTAATGTATCGATTCCTAATAACCCTGCCATACTCTGCTGCTTTGAGAACTTTCTTTCCACTACTGAAAATCTAGTACTGCACAGGGAGAAGGTCCCGGAAGCGATCAGCAGAAGTGGCGGGAATTATTCAAAGCTGCCCAAGCTCAATAACTGGATTCTTAAACTCTATTTGGTGCATCCATATGTACCAGTGATACTGCTTTATAACAAGGATTACAATGGTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

418

Amino Acids

45.8

Weight (kDa)

6.61

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 73 - 151 2.8e-08 NmrA-like family
Epimerase PF01370 76 - 196 9.7e-07 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 77 - 200 1.5e-06 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 80 - 288 4.1e-27 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014247)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31530 AT4G31530
fragaria_vesca FvH4_3g28150
malus_domestica MD03G1169200.v1.1
prunus_persica Prupe.4G258400_v2.0.a1 Prupe.4G258400_v2.0.a1
pyrus_communis pycom03g12540
rosa_chinensis RchiOBHm_Chr5g0050831
rosa_laevigata RLG00000034742
rosa_multiflora Rmu_sc0007394.1_g000008
rosa_roxburghii Rroxscaffold_1G00029880
rosa_rugosa Rorug05G0264500.1
rosa_samantha Rh5AG336500 Rh5BG346000 Rh5CG373700 Rh5DG359000
rosa_wichuraiana Rw5G031730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1230
Acc36I ACCTGC 1 cut(s) 358
AccI GTMKAC 1 cut(s) 682
AccII CGCG 1 cut(s) 124
AciI CCGC 4 cut(s) 134, 246, 249, 1143
AclWI GGATC 2 cut(s) 291, 401
AcoI YGGCCR 1 cut(s) 769
AcsI RAATTY 1 cut(s) 526
AcuI CTGAAG 4 cut(s) 171, 575, 861, 897
AfaI GTAC 2 cut(s) 1101, 1212
AfeI AGCGCT 1 cut(s) 476
AfiI CCNNNNNNNGG 1 cut(s) 86
AflIII ACRYGT 1 cut(s) 433
AgsI TTSAA 6 cut(s) 41, 155, 376, 598, 893, 1155
AhdI GACNNNNNGTC 1 cut(s) 782
AhlI ACTAGT 1 cut(s) 808
AjiI CACGTC 1 cut(s) 779
AloI GAACNNNNNNTCC 2 cut(s) 1064, 1096
AluBI AGCT 8 cut(s) 185, 223, 262, 761, 767, 845, 1159, 1168
AluI AGCT 8 cut(s) 185, 223, 262, 761, 767, 845, 1159, 1168
Alw26I GTCTC 1 cut(s) 303
AlwI GGATC 2 cut(s) 291, 401
AlwNI CAGNNNCTG 1 cut(s) 859
Aor51HI AGCGCT 1 cut(s) 476
AoxI GGCC 1 cut(s) 769
ApeKI GCWGC 3 cut(s) 444, 1062, 1159
ApoI RAATTY 1 cut(s) 526
Asp700I GAANNNNTTC 2 cut(s) 626, 663
AspLEI GCGC 2 cut(s) 126, 477
AspS9I GGNCC 2 cut(s) 722, 1117
AsuC2I CCSGG 1 cut(s) 1121
AsuHPI GGTGA 1 cut(s) 356
AvaII GGWCC 2 cut(s) 722, 1117
BaeI ACNNNNGTAYC 2 cut(s) 795, 828
BalI TGGCCA 1 cut(s) 771
BarI GAAGNNNNNNTAC 2 cut(s) 834, 866
BbsI GAAGAC 1 cut(s) 666
BbvI GCAGC 3 cut(s) 431, 1049, 1146
BccI CCATC 3 cut(s) 418, 646, 713
BcnI CCSGG 1 cut(s) 1121
BcoDI GTCTC 1 cut(s) 303
BcuI ACTAGT 1 cut(s) 808
BfaI CTAG 3 cut(s) 404, 809, 1097
BfmI CTRYAG 2 cut(s) 456, 762
BfoI RGCGCY 1 cut(s) 478
BfuAI ACCTGC 1 cut(s) 358
BglII AGATCT 1 cut(s) 400
BisI GCNGC 4 cut(s) 247, 445, 1063, 1160
BlsI GCNGC 4 cut(s) 248, 446, 1064, 1161
BmcAI AGTACT 1 cut(s) 1101
Bme1390I CCNGG 1 cut(s) 1121
Bme18I GGWCC 2 cut(s) 722, 1117
BmeRI GACNNNNNGTC 1 cut(s) 782
BmgBI CACGTC 1 cut(s) 779
BmgT120I GGNCC 2 cut(s) 722, 1117
BmiI GGNNCC 1 cut(s) 1119
BmrFI CCNGG 1 cut(s) 1121
BmsI GCATC 1 cut(s) 1210
BpiI GAAGAC 1 cut(s) 666
BpmI CTGGAG 2 cut(s) 158, 499
BpuEI CTTGAG 1 cut(s) 623
BpuMI CCSGG 1 cut(s) 1121
Bsa29I ATCGAT 1 cut(s) 1034
BsaBI GATNNNNATC 1 cut(s) 405
BsaJI CCNNGG 2 cut(s) 545, 608
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 5 cut(s) 175, 454, 864, 1181, 1214
Bse3DI GCAATG 3 cut(s) 275, 362, 749
Bse8I GATNNNNATC 1 cut(s) 405
BseCI ATCGAT 1 cut(s) 1034
BseDI CCNNGG 2 cut(s) 545, 608
BseGI GGATG 1 cut(s) 1201
BseJI GATNNNNATC 1 cut(s) 405
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMI GCAATG 3 cut(s) 275, 362, 749
BseMII CTCAG 2 cut(s) 312, 915
BseNI ACTGG 5 cut(s) 175, 454, 864, 1181, 1214
BseXI GCAGC 3 cut(s) 431, 1049, 1146
BseYI CCCAGC 1 cut(s) 181
BsgI GTGCAG 4 cut(s) 430, 522, 801, 1088
Bsh1236I CGCG 1 cut(s) 124
BshFI GGCC 1 cut(s) 771
BshVI ATCGAT 1 cut(s) 1034
BsiSI CCGG 1 cut(s) 1121
BslFI GGGAC 3 cut(s) 163, 395, 1103
BslI CCNNNNNNNGG 1 cut(s) 86
BsmAI GTCTC 1 cut(s) 303
BsmFI GGGAC 3 cut(s) 163, 395, 1103
BsmI GAATGC 1 cut(s) 875
BsnI GGCC 1 cut(s) 771
Bsp143I GATC 5 cut(s) 283, 400, 406, 739, 1128
Bsp19I CCATGG 1 cut(s) 608
BspACI CCGC 4 cut(s) 134, 246, 249, 1143
BspANI GGCC 1 cut(s) 771
BspCNI CTCAG 2 cut(s) 313, 914
BspDI ATCGAT 1 cut(s) 1034
BspFNI CGCG 1 cut(s) 124
BspLI GGNNCC 1 cut(s) 1119
BspMI ACCTGC 1 cut(s) 358
BspPI GGATC 2 cut(s) 291, 401
BsrDI GCAATG 3 cut(s) 275, 362, 749
BsrI ACTGG 5 cut(s) 175, 454, 864, 1181, 1214
BssECI CCNNGG 2 cut(s) 545, 608
BssMI GATC 5 cut(s) 283, 400, 406, 739, 1128
BssT1I CCWWGG 1 cut(s) 608
Bst6I CTCTTC 4 cut(s) 81, 87, 551, 632
BstAPI GCANNNNNTGC 1 cut(s) 146
BstC8I GCNNGC 2 cut(s) 769, 847
BstDEI CTNAG 2 cut(s) 321, 901
BstDSI CCRYGG 1 cut(s) 608
BstF5I GGATG 1 cut(s) 1201
BstFNI CGCG 1 cut(s) 124
BstH2I RGCGCY 1 cut(s) 478
BstHHI GCGC 2 cut(s) 126, 477
BstKTI GATC 5 cut(s) 286, 403, 409, 742, 1131
BstMAI GTCTC 1 cut(s) 303
BstMBI GATC 5 cut(s) 283, 400, 406, 739, 1128
BstMWI GCNNNNNNNGC 3 cut(s) 146, 1140, 1165
BstNSI RCATGY 1 cut(s) 437
BstSCI CCNGG 1 cut(s) 1119
BstSFI CTRYAG 2 cut(s) 456, 762
BstUI CGCG 1 cut(s) 124
BstV1I GCAGC 3 cut(s) 431, 1049, 1146
BstV2I GAAGAC 1 cut(s) 666
BstX2I RGATCY 2 cut(s) 400, 406
BstYI RGATCY 2 cut(s) 400, 406
Bsu15I ATCGAT 1 cut(s) 1034
BsuRI GGCC 1 cut(s) 771
BsuTUI ATCGAT 1 cut(s) 1034
BtgI CCRYGG 1 cut(s) 608
BtrI CACGTC 1 cut(s) 779
BtsCI GGATG 1 cut(s) 1201
BtsI GCAGTG 2 cut(s) 27, 539
BtsIMutI CAGTG 8 cut(s) 27, 318, 447, 539, 551, 857, 873, 1221
BveI ACCTGC 1 cut(s) 358
Cac8I GCNNGC 2 cut(s) 769, 847
CaiI CAGNNNCTG 1 cut(s) 859
CfoI GCGC 2 cut(s) 126, 477
Cfr13I GGNCC 2 cut(s) 722, 1117
ClaI ATCGAT 1 cut(s) 1034
Csp6I GTAC 2 cut(s) 1100, 1211
CviAII CATG 3 cut(s) 146, 434, 609
CviQI GTAC 2 cut(s) 1100, 1211
DdeI CTNAG 2 cut(s) 321, 901
DpnI GATC 5 cut(s) 285, 402, 408, 741, 1130
DpnII GATC 5 cut(s) 283, 400, 406, 739, 1128
DriI GACNNNNNGTC 1 cut(s) 782
EaeI YGGCCR 1 cut(s) 769
Eam1104I CTCTTC 4 cut(s) 81, 87, 551, 632
Eam1105I GACNNNNNGTC 1 cut(s) 782
EarI CTCTTC 4 cut(s) 81, 87, 551, 632
Eco130I CCWWGG 1 cut(s) 608
Eco47I GGWCC 2 cut(s) 722, 1117
Eco47III AGCGCT 1 cut(s) 476
Eco57I CTGAAG 4 cut(s) 171, 575, 861, 897
EcoO109I RGGNCCY 1 cut(s) 1117
EcoT14I CCWWGG 1 cut(s) 608
ErhI CCWWGG 1 cut(s) 608
FaeI CATG 3 cut(s) 149, 437, 612
FalI AAGNNNNNCTT 2 cut(s) 651, 683
FaqI GGGAC 3 cut(s) 163, 395, 1103
FatI CATG 3 cut(s) 145, 433, 608
FauI CCCGC 2 cut(s) 127, 1136
FauNDI CATATG 2 cut(s) 736, 1207
FblI GTMKAC 1 cut(s) 682
Fnu4HI GCNGC 4 cut(s) 247, 445, 1063, 1160
FokI GGATG 1 cut(s) 1188
Fsp4HI GCNGC 4 cut(s) 247, 445, 1063, 1160
FspBI CTAG 3 cut(s) 404, 809, 1097
GlaI GCGC 2 cut(s) 125, 476
GluI GCNGC 4 cut(s) 247, 445, 1063, 1160
GsaI CCCAGC 1 cut(s) 185
GsuI CTGGAG 2 cut(s) 158, 499
HaeII RGCGCY 1 cut(s) 478
HaeIII GGCC 1 cut(s) 771
HapII CCGG 1 cut(s) 1121
HhaI GCGC 2 cut(s) 126, 477
Hin1II CATG 3 cut(s) 149, 437, 612
Hin6I GCGC 2 cut(s) 124, 475
HinP1I GCGC 2 cut(s) 124, 475
HincII GTYRAC 1 cut(s) 898
HindII GTYRAC 1 cut(s) 898
HindIII AAGCTT 1 cut(s) 843
HinfI GANTC 5 cut(s) 12, 191, 1020, 1036, 1180
HpaII CCGG 1 cut(s) 1121
HphI GGTGA 1 cut(s) 356
Hpy166II GTNNAC 3 cut(s) 347, 683, 898
Hpy188I TCNGA 3 cut(s) 11, 190, 288
Hpy188III TCNNGA 1 cut(s) 404
Hpy8I GTNNAC 3 cut(s) 347, 683, 898
Hpy99I CGWCG 3 cut(s) 236, 239, 780
HpyAV CCTTC 3 cut(s) 216, 872, 1108
HpyCH4IV ACGT 2 cut(s) 778, 938
HpyCH4V TGCA 9 cut(s) 335, 367, 447, 539, 782, 849, 873, 1105, 1201
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 1140, 1165
HpyF3I CTNAG 2 cut(s) 321, 901
HpySE526I ACGT 2 cut(s) 778, 938
Hsp92II CATG 3 cut(s) 149, 437, 612
HspAI GCGC 2 cut(s) 124, 475
Kzo9I GATC 5 cut(s) 283, 400, 406, 739, 1128
LmnI GCTCC 3 cut(s) 113, 137, 160
Lsp1109I GCAGC 3 cut(s) 431, 1049, 1146
LweI GCATC 1 cut(s) 1210
MaeI CTAG 3 cut(s) 404, 809, 1097
MaeII ACGT 2 cut(s) 778, 938
MaeIII GTNAC 4 cut(s) 314, 427, 549, 586
MalI GATC 5 cut(s) 285, 402, 408, 741, 1130
MboI GATC 5 cut(s) 283, 400, 406, 739, 1128
MboII GAAGA 9 cut(s) 68, 74, 201, 410, 568, 619, 659, 661, 671
MflI RGATCY 2 cut(s) 400, 406
MlsI TGGCCA 1 cut(s) 771
MluCI AATT 8 cut(s) 34, 68, 112, 196, 526, 561, 673, 1147
MluNI TGGCCA 1 cut(s) 771
MlyI GAGTC 2 cut(s) 6, 1014
MmeI TCCRAC 2 cut(s) 793, 997
Mox20I TGGCCA 1 cut(s) 771
MroXI GAANNNNTTC 2 cut(s) 626, 663
MscI TGGCCA 1 cut(s) 771
MseI TTAA 5 cut(s) 98, 744, 1026, 1185, 1252
MslI CAYNNNNRTG 1 cut(s) 1206
Msp20I TGGCCA 1 cut(s) 771
MspA1I CMGCKG 2 cut(s) 262, 767
MspI CCGG 1 cut(s) 1121
MspR9I CCNGG 1 cut(s) 1121
Mva1269I GAATGC 1 cut(s) 875
MvnI CGCG 1 cut(s) 124
MwoI GCNNNNNNNGC 3 cut(s) 146, 1140, 1165
NciI CCSGG 1 cut(s) 1121
NcoI CCATGG 1 cut(s) 608
NdeI CATATG 2 cut(s) 736, 1207
NdeII GATC 5 cut(s) 283, 400, 406, 739, 1128
NlaIII CATG 3 cut(s) 149, 437, 612
NlaIV GGNNCC 1 cut(s) 1119
NmuCI GTSAC 2 cut(s) 314, 549
NspI RCATGY 1 cut(s) 437
PciI ACATGT 1 cut(s) 433
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PctI GAATGC 1 cut(s) 875
PdmI GAANNNNTTC 2 cut(s) 626, 663
PfeI GAWTC 3 cut(s) 191, 1036, 1180
PfoI TCCNGGA 1 cut(s) 1119
PkrI GCNGC 4 cut(s) 248, 446, 1064, 1161
PleI GAGTC 2 cut(s) 6, 1014
PpsI GAGTC 2 cut(s) 6, 1014
PpuMI RGGWCCY 1 cut(s) 1117
PscI ACATGT 1 cut(s) 433
PsiI TTATAA 1 cut(s) 1230
Psp5II RGGWCCY 1 cut(s) 1117
PspFI CCCAGC 1 cut(s) 181
PspN4I GGNNCC 1 cut(s) 1119
PspPI GGNCC 2 cut(s) 722, 1117
PspPPI RGGWCCY 1 cut(s) 1117
PstNI CAGNNNCTG 1 cut(s) 859
PsuI RGATCY 2 cut(s) 400, 406
PvuII CAGCTG 2 cut(s) 262, 767
RsaI GTAC 2 cut(s) 1101, 1212
RsaNI GTAC 2 cut(s) 1100, 1211
RseI CAYNNNNRTG 1 cut(s) 1206
SaqAI TTAA 5 cut(s) 98, 744, 1026, 1185, 1252
SatI GCNGC 4 cut(s) 247, 445, 1063, 1160
Sau3AI GATC 5 cut(s) 283, 400, 406, 739, 1128
Sau96I GGNCC 2 cut(s) 722, 1117
ScaI AGTACT 1 cut(s) 1101
SchI GAGTC 2 cut(s) 6, 1014
ScrFI CCNGG 1 cut(s) 1121
SfaNI GCATC 1 cut(s) 1210
SfcI CTRYAG 2 cut(s) 456, 762
SinI GGWCC 2 cut(s) 722, 1117
SmiMI CAYNNNNRTG 1 cut(s) 1206
SmlI CTYRAG 1 cut(s) 638
SmoI CTYRAG 1 cut(s) 638
SpeI ACTAGT 1 cut(s) 808
Sse9I AATT 8 cut(s) 34, 68, 112, 196, 526, 561, 673, 1147
SsiI CCGC 4 cut(s) 134, 246, 249, 1143
SspI AATATT 1 cut(s) 888
SspMI CTAG 3 cut(s) 404, 809, 1097
StyD4I CCNGG 1 cut(s) 1119
StyI CCWWGG 1 cut(s) 608
TaiI ACGT 2 cut(s) 781, 941
TaqI TCGA 3 cut(s) 64, 470, 1034
TaqII GACCGA 1 cut(s) 537
TasI AATT 8 cut(s) 34, 68, 112, 196, 526, 561, 673, 1147
TatI WGTACW 1 cut(s) 1099
TauI GCSGC 1 cut(s) 249
TfiI GAWTC 3 cut(s) 191, 1036, 1180
Tru1I TTAA 5 cut(s) 98, 744, 1026, 1185, 1252
Tru9I TTAA 5 cut(s) 98, 744, 1026, 1185, 1252
TscAI CASTG 8 cut(s) 34, 325, 454, 546, 558, 864, 880, 1221
TseFI GTSAC 2 cut(s) 314, 549
TseI GCWGC 3 cut(s) 444, 1062, 1159
Tsp45I GTSAC 2 cut(s) 314, 549
TspDTI ATGAA 4 cut(s) 134, 264, 615, 635
TspRI CASTG 8 cut(s) 34, 325, 454, 546, 558, 864, 880, 1221
VpaK11BI GGWCC 2 cut(s) 722, 1117
XapI RAATTY 1 cut(s) 526
XbaI TCTAGA 1 cut(s) 403
XceI RCATGY 1 cut(s) 437
XmiI GTMKAC 1 cut(s) 682
XmnI GAANNNNTTC 2 cut(s) 626, 663
XspI CTAG 3 cut(s) 404, 809, 1097
ZrmI AGTACT 1 cut(s) 1101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.