Rh5AG336500

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0014247
Physical Location & Seq
Reverse (-)
54088475 .. 54089911
1437 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG336500.1

Sequence Viewer

Length: 618 bp
ATGGCTTTCAGACTCTCACTCACTTCCACTCCCAATTTTCAAATCTCAACCCCAAAACACCCTCGACAATTACCTCTTCCTCTTCTGGGTTTTCAGTTAAAATGTTGCTCCAATTCCATTTCGCGCTCAAAAGCGGGAGCAGTTCATGCTGTTCAACAGGAGCAGGAGGTTCTCCAGTCCCCAGCTTCAGAGTCCAATTCTGTTCCTTCTTCCTCGTCCAAGCTCGTTCTCGTCGTCGGTGGTACCGGCGGTGTTGGTCAGCTGGTAGTGGCTTCATTGCTGGATCGGAATATCAAGTCTCGCCTACTATTGCGTGACACTGAGAAAGCAACTGCATTATTTGGTGAACATGACCAAGAAACATTGCAGGTATTCAAAGGGGACACTCGCAACCCAGAAGATCTAGATCCATCTATATTTGAGGGAGTCACACATGTGATTTGCTGCACTGGAACTACAGCCTTTCCCTCGAAGCGTTGGAGTGGGGATAATACACCAGAAAAAGTTGATTGGGAGGGTGTGAGAAATTTGGTATCTGCACTGCCTCGGTCACTGAAGAGAATTGTTCTTGTATCATCAGTTGGTGTAACCAAGTTCAATGAACTGCCATGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

205

Amino Acids

22.32

Weight (kDa)

9.0

Isoelectric Point (pI)

48.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 73 - 151 2.8e-09 NmrA-like family
Epimerase PF01370 76 - 196 8.7e-08 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 77 - 197 1e-06 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 80 - 198 7.7e-15 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014247)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31530 AT4G31530
fragaria_vesca FvH4_3g28150
malus_domestica MD03G1169200.v1.1
prunus_persica Prupe.4G258400_v2.0.a1 Prupe.4G258400_v2.0.a1
pyrus_communis pycom03g12540
rosa_chinensis RchiOBHm_Chr5g0050831
rosa_laevigata RLG00000034742
rosa_multiflora Rmu_sc0007394.1_g000008
rosa_roxburghii Rroxscaffold_1G00029880
rosa_rugosa Rorug05G0264500.1
rosa_samantha Rh5AG336500 Rh5BG346000 Rh5CG373700 Rh5DG359000
rosa_wichuraiana Rw5G031730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 358
Acc65I GGTACC 1 cut(s) 242
AccB1I GGYRCC 1 cut(s) 242
AccII CGCG 1 cut(s) 124
AciI CCGC 2 cut(s) 134, 249
AclWI GGATC 2 cut(s) 291, 401
AcsI RAATTY 1 cut(s) 526
AcuI CTGAAG 2 cut(s) 171, 575
AfaI GTAC 1 cut(s) 244
AfiI CCNNNNNNNGG 1 cut(s) 86
AflIII ACRYGT 1 cut(s) 433
AgsI TTSAA 4 cut(s) 41, 155, 376, 598
AleI CACNNNNGTG 1 cut(s) 434
AluBI AGCT 3 cut(s) 185, 223, 262
AluI AGCT 3 cut(s) 185, 223, 262
Alw26I GTCTC 1 cut(s) 303
AlwI GGATC 2 cut(s) 291, 401
ApeKI GCWGC 1 cut(s) 444
ApoI RAATTY 1 cut(s) 526
Asp718I GGTACC 1 cut(s) 242
AspLEI GCGC 1 cut(s) 126
AsuHPI GGTGA 1 cut(s) 356
BanI GGYRCC 1 cut(s) 242
BbvI GCAGC 1 cut(s) 431
BccI CCATC 1 cut(s) 418
BcoDI GTCTC 1 cut(s) 303
BfaI CTAG 1 cut(s) 404
BfmI CTRYAG 1 cut(s) 456
BfuAI ACCTGC 1 cut(s) 358
BglII AGATCT 1 cut(s) 400
BisI GCNGC 1 cut(s) 445
BlsI GCNGC 1 cut(s) 446
BmiI GGNNCC 1 cut(s) 244
BpmI CTGGAG 1 cut(s) 158
BsaBI GATNNNNATC 1 cut(s) 405
BsaJI CCNNGG 2 cut(s) 545, 608
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 2 cut(s) 175, 454
Bse3DI GCAATG 2 cut(s) 275, 362
Bse8I GATNNNNATC 1 cut(s) 405
BseDI CCNNGG 2 cut(s) 545, 608
BseJI GATNNNNATC 1 cut(s) 405
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMI GCAATG 2 cut(s) 275, 362
BseMII CTCAG 1 cut(s) 312
BseNI ACTGG 2 cut(s) 175, 454
BseXI GCAGC 1 cut(s) 431
BseYI CCCAGC 1 cut(s) 181
BsgI GTGCAG 2 cut(s) 430, 522
Bsh1236I CGCG 1 cut(s) 124
BshNI GGYRCC 1 cut(s) 242
BsiSI CCGG 1 cut(s) 246
BslFI GGGAC 2 cut(s) 163, 395
BslI CCNNNNNNNGG 1 cut(s) 86
BsmAI GTCTC 1 cut(s) 303
BsmFI GGGAC 2 cut(s) 163, 395
Bsp143I GATC 3 cut(s) 283, 400, 406
Bsp19I CCATGG 1 cut(s) 608
BspACI CCGC 2 cut(s) 134, 249
BspCNI CTCAG 1 cut(s) 313
BspFNI CGCG 1 cut(s) 124
BspLI GGNNCC 1 cut(s) 244
BspMI ACCTGC 1 cut(s) 358
BspPI GGATC 2 cut(s) 291, 401
BspT107I GGYRCC 1 cut(s) 242
BsrDI GCAATG 2 cut(s) 275, 362
BsrFI RCCGGY 1 cut(s) 245
BsrI ACTGG 2 cut(s) 175, 454
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 2 cut(s) 545, 608
BssMI GATC 3 cut(s) 283, 400, 406
BssT1I CCWWGG 1 cut(s) 608
Bst6I CTCTTC 3 cut(s) 81, 87, 551
BstAPI GCANNNNNTGC 1 cut(s) 146
BstDEI CTNAG 1 cut(s) 321
BstDSI CCRYGG 1 cut(s) 608
BstFNI CGCG 1 cut(s) 124
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 3 cut(s) 286, 403, 409
BstMAI GTCTC 1 cut(s) 303
BstMBI GATC 3 cut(s) 283, 400, 406
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNSI RCATGY 1 cut(s) 437
BstSFI CTRYAG 1 cut(s) 456
BstUI CGCG 1 cut(s) 124
BstV1I GCAGC 1 cut(s) 431
BstX2I RGATCY 2 cut(s) 400, 406
BstYI RGATCY 2 cut(s) 400, 406
BtgI CCRYGG 1 cut(s) 608
BtsI GCAGTG 1 cut(s) 539
BtsIMutI CAGTG 4 cut(s) 318, 447, 539, 551
BveI ACCTGC 1 cut(s) 358
CfoI GCGC 1 cut(s) 126
Cfr10I RCCGGY 1 cut(s) 245
Csp6I GTAC 1 cut(s) 243
CviAII CATG 4 cut(s) 146, 350, 434, 609
CviJI RGCY 6 cut(s) 5, 185, 223, 262, 272, 461
CviKI_1 RGCY 6 cut(s) 5, 185, 223, 262, 272, 461
CviQI GTAC 1 cut(s) 243
DdeI CTNAG 1 cut(s) 321
DpnI GATC 3 cut(s) 285, 402, 408
DpnII GATC 3 cut(s) 283, 400, 406
Eam1104I CTCTTC 3 cut(s) 81, 87, 551
EarI CTCTTC 3 cut(s) 81, 87, 551
Eco130I CCWWGG 1 cut(s) 608
Eco57I CTGAAG 2 cut(s) 171, 575
EcoT14I CCWWGG 1 cut(s) 608
ErhI CCWWGG 1 cut(s) 608
FaeI CATG 4 cut(s) 149, 353, 437, 612
FaiI YATR 5 cut(s) 147, 351, 416, 435, 610
FaqI GGGAC 2 cut(s) 163, 395
FatI CATG 4 cut(s) 145, 349, 433, 608
FauI CCCGC 1 cut(s) 127
Fnu4HI GCNGC 1 cut(s) 445
Fsp4HI GCNGC 1 cut(s) 445
FspBI CTAG 1 cut(s) 404
GlaI GCGC 1 cut(s) 125
GluI GCNGC 1 cut(s) 445
GsaI CCCAGC 1 cut(s) 185
GsuI CTGGAG 1 cut(s) 158
HapII CCGG 1 cut(s) 246
HhaI GCGC 1 cut(s) 126
Hin1II CATG 4 cut(s) 149, 353, 437, 612
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HinfI GANTC 3 cut(s) 12, 191, 426
HpaII CCGG 1 cut(s) 246
HphI GGTGA 1 cut(s) 356
Hpy166II GTNNAC 1 cut(s) 347
Hpy188I TCNGA 3 cut(s) 11, 190, 288
Hpy188III TCNNGA 1 cut(s) 404
Hpy8I GTNNAC 1 cut(s) 347
Hpy99I CGWCG 2 cut(s) 236, 239
HpyAV CCTTC 1 cut(s) 216
HpyCH4V TGCA 4 cut(s) 335, 367, 447, 539
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 1 cut(s) 321
Hsp92II CATG 4 cut(s) 149, 353, 437, 612
HspAI GCGC 1 cut(s) 124
KpnI GGTACC 1 cut(s) 246
Kzo9I GATC 3 cut(s) 283, 400, 406
LmnI GCTCC 3 cut(s) 113, 137, 160
Lsp1109I GCAGC 1 cut(s) 431
MaeI CTAG 1 cut(s) 404
MaeIII GTNAC 4 cut(s) 314, 427, 549, 586
MalI GATC 3 cut(s) 285, 402, 408
MboI GATC 3 cut(s) 283, 400, 406
MboII GAAGA 5 cut(s) 68, 74, 201, 410, 568
MflI RGATCY 2 cut(s) 400, 406
MluCI AATT 6 cut(s) 34, 68, 112, 196, 526, 561
MlyI GAGTC 3 cut(s) 6, 200, 435
MmeI TCCRAC 1 cut(s) 458
MseI TTAA 1 cut(s) 98
MslI CAYNNNNRTG 1 cut(s) 434
MspA1I CMGCKG 1 cut(s) 262
MspI CCGG 1 cut(s) 246
MvnI CGCG 1 cut(s) 124
MwoI GCNNNNNNNGC 1 cut(s) 146
NcoI CCATGG 1 cut(s) 608
NdeII GATC 3 cut(s) 283, 400, 406
NlaIII CATG 4 cut(s) 149, 353, 437, 612
NlaIV GGNNCC 1 cut(s) 244
NmuCI GTSAC 3 cut(s) 314, 427, 549
NspI RCATGY 1 cut(s) 437
OliI CACNNNNGTG 1 cut(s) 434
PciI ACATGT 1 cut(s) 433
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PkrI GCNGC 1 cut(s) 446
PleI GAGTC 3 cut(s) 6, 199, 434
PpsI GAGTC 3 cut(s) 6, 199, 434
PscI ACATGT 1 cut(s) 433
PspFI CCCAGC 1 cut(s) 181
PspN4I GGNNCC 1 cut(s) 244
PsuI RGATCY 2 cut(s) 400, 406
PvuII CAGCTG 1 cut(s) 262
RsaI GTAC 1 cut(s) 244
RsaNI GTAC 1 cut(s) 243
RseI CAYNNNNRTG 1 cut(s) 434
SaqAI TTAA 1 cut(s) 98
SatI GCNGC 1 cut(s) 445
Sau3AI GATC 3 cut(s) 283, 400, 406
SchI GAGTC 3 cut(s) 6, 200, 435
SetI ASST 7 cut(s) 76, 171, 187, 225, 264, 372, 617
SfcI CTRYAG 1 cut(s) 456
SmiMI CAYNNNNRTG 1 cut(s) 434
Sse9I AATT 6 cut(s) 34, 68, 112, 196, 526, 561
SsiI CCGC 2 cut(s) 134, 249
SspMI CTAG 1 cut(s) 404
StyI CCWWGG 1 cut(s) 608
TaqI TCGA 2 cut(s) 64, 470
TaqII GACCGA 1 cut(s) 537
TasI AATT 6 cut(s) 34, 68, 112, 196, 526, 561
Tru1I TTAA 1 cut(s) 98
Tru9I TTAA 1 cut(s) 98
TscAI CASTG 4 cut(s) 325, 454, 546, 558
TseFI GTSAC 3 cut(s) 314, 427, 549
TseI GCWGC 1 cut(s) 444
Tsp45I GTSAC 3 cut(s) 314, 427, 549
TspDTI ATGAA 3 cut(s) 134, 264, 615
TspRI CASTG 4 cut(s) 325, 454, 546, 558
XapI RAATTY 1 cut(s) 526
XbaI TCTAGA 1 cut(s) 403
XceI RCATGY 1 cut(s) 437
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.