MD03G1184300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014185
Physical Location & Seq
Reverse (-)
25119468 .. 25121650
2183 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1184300.v1.1.491

Sequence Viewer

Length: 438 bp
ATGGACCCCTCTCCAGTGCGAACCACTCCACCTCTCGAAGAAGAAGATGAGTGGGACACTGATGGGTTCGTGATTCCGAGCTTGGTGGTTGAAGAGCAAGATAAACAAAATCAGGATGCTCCAACAGTGGAAGCTTCAAAACCTCCATCTCCGAAGGCGGAAAAAGAAGAGAAGATCTACTTAGGACCGCACGGGGCTCCTCCTTCGCAATCAAAGCAGCAAGAGGTAAACCCTGGTGGTCGTAAGCAGAAGTTCAAGCAGAAACTGAAGGAAGCGGATAGGAAAAGTACCGGGGCAGGCCGAGAGAATAAGTTGGAGAATCTGAGAGAGCTTGTGGGCGATGGGAAAGGAAGCAGCAGCATGGCAAAGGGTTCTAACAGGGATTGGTTAGACCCACACTGCCATGAGTCTGAGTTCGAGAAGTGGAACAGCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

16.23

Weight (kDa)

5.34

Isoelectric Point (pI)

58.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55640
fragaria_vesca FvH4_3g29370
malus_domestica MD03G1184300.v1.1 MD11G1201100.v1.1
prunus_persica Prupe.4G242300_v2.0.a1 Prupe.4G242300_v2.0.a1
pyrus_communis pycom11g17350
rosa_chinensis RchiOBHm_Chr5g0053761
rosa_laevigata RLG00000034901
rosa_multiflora Rmu_sc0001759.1_g000025
rosa_roxburghii Rroxscaffold_1G00026830
rosa_rugosa Rorug05G0282400
rosa_samantha Rh5AG352900 Rh5BG364600 Rh5CG389500 Rh5DG379400
rosa_wichuraiana Rw5G033340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 158, 188, 275
AcuI CTGAAG 1 cut(s) 287
AfaI GTAC 1 cut(s) 289
AgsI TTSAA 3 cut(s) 92, 138, 256
AjnI CCWGG 1 cut(s) 232
AluBI AGCT 3 cut(s) 81, 134, 331
AluI AGCT 3 cut(s) 81, 134, 331
AlwNI CAGNNNCTG 1 cut(s) 265
AoxI GGCC 1 cut(s) 298
ApeKI GCWGC 3 cut(s) 217, 354, 357
AspS9I GGNCC 2 cut(s) 4, 185
AsuC2I CCSGG 1 cut(s) 292
AvaII GGWCC 2 cut(s) 4, 185
BanII GRGCYC 1 cut(s) 199
BbvI GCAGC 3 cut(s) 229, 366, 369
BccI CCATC 3 cut(s) 56, 154, 335
BciT130I CCWGG 1 cut(s) 234
BcnI CCSGG 1 cut(s) 292
BglII AGATCT 1 cut(s) 174
BisI GCNGC 3 cut(s) 218, 355, 358
BlsI GCNGC 3 cut(s) 219, 356, 359
Bme1390I CCNGG 2 cut(s) 234, 292
Bme18I GGWCC 2 cut(s) 4, 185
BmgT120I GGNCC 2 cut(s) 4, 185
BmiI GGNNCC 2 cut(s) 6, 198
BmrFI CCNGG 2 cut(s) 234, 292
BmsI GCATC 1 cut(s) 106
BpuMI CCSGG 1 cut(s) 292
BsaJI CCNNGG 2 cut(s) 232, 291
Bse118I RCCGGY 1 cut(s) 432
Bse1I ACTGG 1 cut(s) 14
BseBI CCWGG 1 cut(s) 234
BseDI CCNNGG 2 cut(s) 232, 291
BseGI GGATG 1 cut(s) 121
BseMII CTCAG 2 cut(s) 314, 402
BseNI ACTGG 1 cut(s) 14
BseRI GAGGAG 1 cut(s) 189
BseXI GCAGC 3 cut(s) 229, 366, 369
BshFI GGCC 1 cut(s) 300
BsiSI CCGG 2 cut(s) 291, 433
BslFI GGGAC 1 cut(s) 68
BsmFI GGGAC 1 cut(s) 68
BsnI GGCC 1 cut(s) 300
Bsp1286I GDGCHC 1 cut(s) 199
Bsp143I GATC 1 cut(s) 174
BspACI CCGC 3 cut(s) 158, 188, 275
BspANI GGCC 1 cut(s) 300
BspCNI CTCAG 2 cut(s) 315, 403
BspLI GGNNCC 2 cut(s) 6, 198
BspQI GCTCTTC 1 cut(s) 87
BsrFI RCCGGY 1 cut(s) 432
BsrI ACTGG 1 cut(s) 14
BssAI RCCGGY 1 cut(s) 432
BssECI CCNNGG 2 cut(s) 232, 291
BssMI GATC 1 cut(s) 174
Bst2UI CCWGG 1 cut(s) 234
Bst4CI ACNGT 1 cut(s) 127
Bst6I CTCTTC 2 cut(s) 87, 162
BstC8I GCNNGC 1 cut(s) 298
BstDEI CTNAG 3 cut(s) 181, 323, 411
BstF5I GGATG 1 cut(s) 121
BstKTI GATC 1 cut(s) 177
BstMBI GATC 1 cut(s) 174
BstMWI GCNNNNNNNGC 1 cut(s) 214
BstNI CCWGG 1 cut(s) 234
BstSCI CCNGG 2 cut(s) 232, 290
BstV1I GCAGC 3 cut(s) 229, 366, 369
BstX2I RGATCY 1 cut(s) 174
BstYI RGATCY 1 cut(s) 174
BsuRI GGCC 1 cut(s) 300
BtgZI GCGATG 1 cut(s) 354
BtsCI GGATG 1 cut(s) 121
BtsI GCAGTG 1 cut(s) 397
BtsIMutI CAGTG 4 cut(s) 21, 57, 132, 397
Cac8I GCNNGC 1 cut(s) 298
CaiI CAGNNNCTG 1 cut(s) 265
Cfr10I RCCGGY 1 cut(s) 432
Cfr13I GGNCC 2 cut(s) 4, 185
Csp6I GTAC 1 cut(s) 288
CviAII CATG 2 cut(s) 361, 404
CviJI RGCY 6 cut(s) 81, 134, 197, 300, 331, 432
CviKI_1 RGCY 6 cut(s) 81, 134, 197, 300, 331, 432
CviQI GTAC 1 cut(s) 288
DdeI CTNAG 3 cut(s) 181, 323, 411
DpnI GATC 1 cut(s) 176
DpnII GATC 1 cut(s) 174
Eam1104I CTCTTC 2 cut(s) 87, 162
EarI CTCTTC 2 cut(s) 87, 162
EciI GGCGGA 1 cut(s) 173
Eco24I GRGCYC 1 cut(s) 199
Eco47I GGWCC 2 cut(s) 4, 185
Eco57I CTGAAG 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 232
EcoT38I GRGCYC 1 cut(s) 199
FaeI CATG 2 cut(s) 364, 407
FaiI YATR 2 cut(s) 362, 405
FalI AAGNNNNNCTT 2 cut(s) 164, 196
FaqI GGGAC 1 cut(s) 68
FatI CATG 2 cut(s) 360, 403
Fnu4HI GCNGC 3 cut(s) 218, 355, 358
FokI GGATG 1 cut(s) 128
FriOI GRGCYC 1 cut(s) 199
Fsp4HI GCNGC 3 cut(s) 218, 355, 358
GluI GCNGC 3 cut(s) 218, 355, 358
HaeIII GGCC 1 cut(s) 300
HapII CCGG 2 cut(s) 291, 433
Hin1II CATG 2 cut(s) 364, 407
HindIII AAGCTT 1 cut(s) 132
HinfI GANTC 3 cut(s) 73, 319, 407
HpaII CCGG 2 cut(s) 291, 433
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 4 cut(s) 78, 153, 324, 412
Hpy188III TCNNGA 4 cut(s) 35, 70, 113, 418
Hpy8I GTNNAC 1 cut(s) 229
HpyAV CCTTC 3 cut(s) 148, 213, 262
HpyCH4III ACNGT 1 cut(s) 127
HpyF10VI GCNNNNNNNGC 1 cut(s) 214
HpyF3I CTNAG 3 cut(s) 181, 323, 411
Hsp92II CATG 2 cut(s) 364, 407
Kzo9I GATC 1 cut(s) 174
LguI GCTCTTC 1 cut(s) 87
LmnI GCTCC 2 cut(s) 124, 202
LpnPI CCDG 7 cut(s) 27, 98, 219, 246, 282, 304, 364
Lsp1109I GCAGC 3 cut(s) 229, 366, 369
LweI GCATC 1 cut(s) 106
MalI GATC 1 cut(s) 176
MboI GATC 1 cut(s) 174
MboII GAAGA 6 cut(s) 50, 53, 56, 104, 179, 184
MflI RGATCY 1 cut(s) 174
MhlI GDGCHC 1 cut(s) 199
MlyI GAGTC 1 cut(s) 416
MmeI TCCRAC 2 cut(s) 146, 294
MnlI CCTC 5 cut(s) 19, 42, 153, 210, 217
MslI CAYNNNNRTG 1 cut(s) 402
MspI CCGG 2 cut(s) 291, 433
MspR9I CCNGG 2 cut(s) 234, 292
MvaI CCWGG 1 cut(s) 234
MwoI GCNNNNNNNGC 1 cut(s) 214
NciI CCSGG 1 cut(s) 292
NdeII GATC 1 cut(s) 174
NlaIII CATG 2 cut(s) 364, 407
NlaIV GGNNCC 2 cut(s) 6, 198
NmeAIII GCCGAG 1 cut(s) 326
PciSI GCTCTTC 1 cut(s) 87
PfeI GAWTC 2 cut(s) 73, 319
PkrI GCNGC 3 cut(s) 219, 356, 359
PleI GAGTC 1 cut(s) 415
PpsI GAGTC 1 cut(s) 415
Psp6I CCWGG 1 cut(s) 232
PspGI CCWGG 1 cut(s) 232
PspN4I GGNNCC 2 cut(s) 6, 198
PspPI GGNCC 2 cut(s) 4, 185
PstNI CAGNNNCTG 1 cut(s) 265
PsuI RGATCY 1 cut(s) 174
RsaI GTAC 1 cut(s) 289
RsaNI GTAC 1 cut(s) 288
RseI CAYNNNNRTG 1 cut(s) 402
SapI GCTCTTC 1 cut(s) 87
SatI GCNGC 3 cut(s) 218, 355, 358
Sau3AI GATC 1 cut(s) 174
Sau96I GGNCC 2 cut(s) 4, 185
SchI GAGTC 1 cut(s) 416
ScrFI CCNGG 2 cut(s) 234, 292
SduI GDGCHC 1 cut(s) 199
SetI ASST 6 cut(s) 34, 83, 136, 145, 228, 333
SfaNI GCATC 1 cut(s) 106
SinI GGWCC 2 cut(s) 4, 185
SmiMI CAYNNNNRTG 1 cut(s) 402
SsiI CCGC 3 cut(s) 158, 188, 275
StyD4I CCNGG 2 cut(s) 232, 290
TaaI ACNGT 1 cut(s) 127
TaqI TCGA 2 cut(s) 36, 417
TfiI GAWTC 2 cut(s) 73, 319
TscAI CASTG 4 cut(s) 21, 64, 132, 404
TseI GCWGC 3 cut(s) 217, 354, 357
TspRI CASTG 4 cut(s) 21, 64, 132, 404
VpaK11BI GGWCC 2 cut(s) 4, 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.