MD03G1189500.v1.1

Tornado 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015957
Physical Location & Seq
Forward (+)
25990481 .. 25995238
4758 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1189500.v1.1.491

Sequence Viewer

Length: 4137 bp
ATGGCAACAAGCCAGAACAATCTCAAGGATCTCCAATGGCTTCTGGATGCCATCGAATCCGAAAGCCTCAGCCTCCACAACATCTCCTTCTACCTCTCTCAACCCTCTTCCTTCGGCTTCCAGGAAACCGAAAACTCCGTCAACATCAACGTTTCGGAGGACAGCCTCTCTTTCTTCTCCCACTTCCTCACAGCTTTAGCAACATCCAAAACCAACCAACTTCTGATGAACTTGGAGTTTCACCAGGTTCAGTGGGACGTGCAGCAGCTTCGCCATCTTTCGGCGTTGCTTGAGAGTAGTTTGAGCATCAAGCAAGTAGGGTTTAGGAGAAACAGATTTGACAAACAATGCCTGGCAGAGTTGTGTGAGATCGTGAAGAGGAACAGAGTGATCAAAGAGATTATGTTTTCGGAATCAGGTATCGGGTTTGCTGGAGCCGGGTTTCTTGCTTCTGCGCTTAAGGTCAATGAGAGCTTGGAGGAGTTGCAGATATGGGAAGACTCTATTGGGTCGAAAGGCGCAGAGGAGCTCTCGAAGATGATTGAAGTGAACTCAACACTGAAGGTGTTGACAATATTTGACTCGAATTCGATCACTGCAACTCCACTTATTTCTGCAGTTTTAGCAAGGAATAGGTCCATGGAAGTCCATGTTTGGAGTGGAGAAAACGGAGAAAGAAGCTCGAAGGTGGTTGAGTTTCTACCCGAAAACAGCACGCTTAGGATTTACAGGCTCGACCTTTCGGGCGCTTGCAGGGTTGCCTGTGCTCTCGGGTGGAACTCAACGGTTAAGTCCCTCGACCTTACTGGAGTGCGGTTGAAATCAAGGTGGGCTAAGGAGTTTCGGTGGGTTTTGGAACAGAACCAGACCCTCAAAGAGGTGAACTTGTCGAAAACTTGGCTGAAAGATAAGGGGGTTGTGTATGTAGCAGCTGGACTCTTCAAAAACCAGAGCTTGGAGTGCTTGTGCTTAGATGGGAACGCGTTTGGAGGTATCGGAGTTGAGCATTTACTCTGCCCTTTGAGCAGGTTCTCTGCCTTGCAATATCAAGCAAACATTACTTTAAAATCGGTAACACTTGGAGGTGGAAGAACCAAGATTGGAAGGGAGGGGCTTGCAGCAATTTTACAGATGCTGACAACTAATGAAAGTTTGACGCGGTTGGGGATATTCGATGATGAGAGTTTAAGGCCCGATGATTTTGTTAAACTCTTCAAAAGCTTGGAGAAGAATGCCAGCTTGAGACACTTATCGTTACAGGGCTGCAAAGGTGTTCAAGGAGAGCAGGTTCTGCAGGCAATCATGGAGACACTGCAGGTAAATCCTTGGCTTGAAAATATTGATCTTGCAAGAACGCCGCTGCAGAATTCAGGAAAGACAGATGGGGTCTATCAAAGACTCGGGCAGAATGGGAAGCCTGAACCGGAAATGGATTTGCTTAAAGACATGCCACTTACAGTACCGAAAAGTTGTAGAGTGTTCTTCTGTGGGCAAGAATATGCAGGTAAAGCTACATTGTGCAATTCCATATTGCAGAGTGTGTCTTCTTCAAAGCTTCTGTACGTGGACCAAGTACTATCTTTAGTGAACCCGGTTGAGCAAGCTGTTAGAACAGTTGGAATGAAGATTAAGACTTTCAAAGACGATGACAACACAAAGATTTCAATATGGAATCTTGCTGGGCAGCATGAATTCTATTCTCTTCATGATCTCATGTTCCCGGGGCATGGCAGTGCGTCATTTTTTGTCATCGTATCCAGTTTATTCAGGAAAACAAACAACAGAGAACCGAAAAACTCAATGGAGATAGAAGAGGACCTCCAGTACTGGCTCAGGTTCATAGTCTCAAACTCTAGAAGAGCAGTGCAGCAATGCATGCTACCAAATGTCACGGTTGTACTCACGCACTATGACAAAATCAATCAACCGTCACAAAACTTACAGGTTGCAGTGAACTCGATTCAAAAATTGCGAGACAAGTTCCAGGGATTTGTTGACTTCTATGCAACGGTGTTCACAGTTGATGCAAGATCATCAGCATCAGTGAGTAAGCTCACTCATCACCTTCTAAAGACAAGCAAGACAGTTCTCCAAAGAGTCCCAAGAATCTATCAGCTGTGCAATGATCTGACACAAATATTATCAGATTGGAGATCGGAGAACTACAACAAGCCAGCAATGCAGTGGAAGGAGTTCAACGAGCTATGCCAAGTTAAGGTTCCATCATTAAGAGTCCGATCAAGACACGATAACAAAGAGAAGGTGGAAACGAGGAGGCGGGTTGTTGCTACTTGCCTTCACCACATAGGAGAGGTTATTTATTTTGATGAGCTCGGGTTTCTGATCTTAGATTGTGAGTGGTTCTGCGGCGAGGTGCTTGGCCAACTAATAAGACTAGATGTAAGAAGTCGAAACTCCAACGAGAACAATGGATTCATCAGCAAGAAAGATTTGGAGAAGATTCTAAGAGGAAGCTTGCAGAGCCCGATTCCTGGAGTGGGTACGAAGGTATTTGAGAACTTGGATGCAACTGATCTTGTGAGAATGATGCTTAAACTGGAACTATGTTATGAACAAGACCCTTCAGATCGTAATTCTTTGCTGTTGATCCCCTCCATTCTTGAAGAAGGAAGAGGAAAGCCACAACGATGGCACTTAAGCAGACCGGAGTCCCTTTATGCCGGGAGGCATCTTGAATGTGATGATTCCAGCCACATGTTCCTCACACCCGGCTTCTTTCCTCGATTACAGGTGCATCTGCATAACAGAATCATGGCCTTGAAGAACCAACACGGAGCGACTTACAGCCTTGAGAAGTACCTGATCTCGATAACTATCAATGGAATCTATATCAGAGTAGAACTGGGAGGACAGCTGGGTTACTACATCGATATCCTCGCATGCTCCACCAACAACCTGACAGAAACCCTGAGGGTTATCCAGCAGCTTATAATCCCAGCAATTCATAGCCTCTGTAACGGTATCACCTTGACTGAAAATGTCATACGGCCAGAATGCGTGCAAAATCTCACTCCTCCAAGATACAGGAAGATGCAATTTGTTTCGTTGCAACAATTAAAACAGGCATTACTTTCTGTTCCTGCAGACAGCATGTATGATTACCAGCATACCTGGGATCCAATCTCAGACTCCGGAAGACAAATTCTAGGACCTGGGTTTGATCTGGCCCGAGACCTCCTATCAGATGATGACTTCCGTGAAGTGCTCCATCGGAGATATCATGACCTATACAACCTTGCTCAGGAACTGCAAATCCCACCCGAGAGCAACCCGGAGAACACATTATCCACAAGTGACGAGGCTGACAAAGTTGATCCAACTTTCGGTGGAATTGCCAAGGGGGTTGAAGCGGTTTTGCAGAGGCTGAAGATCATTGAGCAAGAAATCAGAGACTTGAAACAGGAGATCCAAGGGTTGAGATATTACGAGCACAGACTCCTTGCAGAGCTTCATCGCAAAGTGAATTACCTTGTGACATACAATGTCCAAATTGAAGAGAGGAAAGTGCCGAATATGTTCTACTTTGTTAGAACAGAAAACTACTCGAGGAGATTGATCACCAATATGGTTCCCGGCATGAATGCTATTCGACTGCACATGTTATGCGAATTTCGACGAGAAATGCATGTTGTTGAAGATCAAATGGGGTGCGAACTGATGCAGGTTGACAACAGGACCGTGAAGTCTTTGGCACCGTACACCACAAAGTTCATGAAACTGCTTACATTTGCTCTCAAGATAGGAGCACATCTAGCCGCAGGCATGGGAGAAATGATACCGGATTTGAGCAGGGAAGTCGCCCACCTGGCTGATTCTTCCCTCCTTTATGGAGCTGCAGGGGCAGTTGCTGCCGGTGCAGTGGGAGCTGCGGCACTTGGGCAGGGAAGGAACAGAAGTAGGGCTGCAGAAAGCTCGAGAGATATCCAGCAAGATCAGAGAACAGCACAACAATGGGTTCTGGATTTCCTGAGGGACCGGAAATGCTCAACCGGAAAGGATATTGCCGAGAAGTTTGGTTTGTGGAGAGTGCGGTACAGAGACGATGGTCAGATTGCTTGGATCTGCAGGAGGCATATTAACCTCAGAGCACATGAAATAATTGAAGTGCCACTTTGA

Protein Analysis

1379

Amino Acids

156.51

Weight (kDa)

7.72

Isoelectric Point (pI)

45.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COR-A PF16095 720 - 874 2.3e-09 C-terminal of Roc, COR-A domain
COR-B PF25497 896 - 1039 9.8e-14 C-terminal of Roc, COR-B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015957)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55540 AT5G55540
fragaria_vesca FvH4_3g23950
malus_domestica MD03G1189500.v1.1
prunus_persica Prupe.4G231800_v2.0.a1
pyrus_communis pycom03g14240
rosa_chinensis RchiOBHm_Chr5g0042451
rosa_laevigata RLG00000034127
rosa_multiflora Rmu_sc0002779.1_g000005 Rmu_sc0016438.1_g000001
rosa_roxburghii Rroxscaffold_1G00038240
rosa_rugosa Rorug05G0200300
rosa_samantha Rh5AG286400 Rh5CG323200
rosa_wichuraiana Rw5G026610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2951
AasI GACNNNNNNGTC 1 cut(s) 3703
Acc36I ACCTGC 5 cut(s) 1017, 1276, 1306, 1493, 3673
AccB1I GGYRCC 1 cut(s) 3712
AccB7I CCANNNNNTGG 1 cut(s) 955
AccII CGCG 2 cut(s) 983, 1159
AccIII TCCGGA 1 cut(s) 3152
AciI CCGC 9 cut(s) 814, 1159, 1358, 2278, 2367, 3371, 3777, 3890, 4051
AclI AACGTT 1 cut(s) 150
AclWI GGATC 7 cut(s) 36, 2602, 3131, 3144, 3329, 3421, 4088
AcoI YGGCCR 2 cut(s) 2380, 3008
AcsI RAATTY 5 cut(s) 586, 1366, 1691, 3162, 3629
AcuI CTGAAG 3 cut(s) 581, 2568, 3407
AfaI GTAC 9 cut(s) 1461, 1562, 1575, 1826, 1899, 2503, 2819, 3719, 4055
AfiI CCNNNNNNNGG 9 cut(s) 280, 877, 955, 1727, 2215, 2492, 2498, 3262, 3344
AflII CTTAAG 2 cut(s) 458, 2656
AflIII ACRYGT 3 cut(s) 981, 2715, 3618
AjiI CACGTC 1 cut(s) 259
AjnI CCWGG 8 cut(s) 120, 243, 351, 1983, 2491, 3131, 3172, 3825
AjuI GAANNNNNNNTTGG 4 cut(s) 489, 521, 2202, 2234
AloI GAACNNNNNNTCC 6 cut(s) 1272, 1304, 3258, 3290, 3290, 3322
Alw21I GWGCWC 7 cut(s) 531, 769, 2334, 3228, 3453, 3769, 4111
Alw26I GTCTC 7 cut(s) 1237, 1301, 1849, 1968, 3186, 3405, 4053
AlwI GGATC 7 cut(s) 36, 2602, 3131, 3144, 3329, 3421, 4088
AlwNI CAGNNNCTG 5 cut(s) 1135, 1291, 3268, 3385, 3869
Ama87I CYCGRG 8 cut(s) 770, 1400, 1720, 2333, 3189, 3281, 3565, 3934
Aor13HI TCCGGA 1 cut(s) 3152
AoxI GGCC 5 cut(s) 1190, 2380, 2775, 3008, 3186
ApoI RAATTY 5 cut(s) 586, 1366, 1691, 3162, 3629
ArsI GACNNNNNNTTYG 2 cut(s) 123, 155
Asp700I GAANNNNTTC 2 cut(s) 2192, 3536
AspLEI GCGC 3 cut(s) 457, 521, 749
AspS9I GGNCC 8 cut(s) 636, 1191, 1567, 1816, 3170, 3187, 3696, 3994
AsuC2I CCSGG 8 cut(s) 439, 1592, 1721, 1722, 2683, 2730, 3293, 3594
AsuHPI GGTGA 6 cut(s) 233, 892, 2054, 2291, 2977, 3571
AvaI CYCGRG 8 cut(s) 770, 1400, 1720, 2333, 3189, 3281, 3565, 3934
AvaII GGWCC 6 cut(s) 636, 1567, 1816, 3170, 3696, 3994
AxyI CCTNAGG 2 cut(s) 2930, 3989
BaeI ACNNNNGTAYC 2 cut(s) 2493, 2526
BalI TGGCCA 1 cut(s) 2382
BamHI GGATCC 1 cut(s) 3136
BanI GGYRCC 1 cut(s) 3712
BanII GRGCYC 3 cut(s) 531, 2334, 2486
BbsI GAAGAC 3 cut(s) 504, 1536, 3163
Bbv12I GWGCWC 7 cut(s) 531, 769, 2334, 3228, 3453, 3769, 4111
BbvCI CCTCAGC 1 cut(s) 68
BccI CCATC 8 cut(s) 59, 282, 968, 1376, 2230, 2643, 3237, 4058
BceAI ACGGC 1 cut(s) 3023
BcgI CGANNNNNNTGC 8 cut(s) 251, 285, 1938, 1972, 3799, 3833, 3915, 3949
BciT130I CCWGG 8 cut(s) 122, 245, 353, 1985, 2493, 3133, 3174, 3827
BciVI GTATCC 1 cut(s) 1765
BclI TGATCA 2 cut(s) 390, 3576
BcnI CCSGG 8 cut(s) 439, 1592, 1721, 1722, 2683, 2730, 3293, 3594
BcoDI GTCTC 7 cut(s) 1237, 1301, 1849, 1968, 3186, 3405, 4053
BfaI CTAG 4 cut(s) 1854, 2396, 3167, 3773
BfmI CTRYAG 8 cut(s) 615, 1292, 1313, 1361, 3102, 3855, 3924, 4084
BfoI RGCGCY 1 cut(s) 750
BfrI CTTAAG 2 cut(s) 458, 2656
BfuAI ACCTGC 5 cut(s) 1017, 1276, 1306, 1493, 3673
BfuI GTATCC 1 cut(s) 1765
BglI GCCNNNNNGGC 1 cut(s) 3827
BmcAI AGTACT 2 cut(s) 1575, 1826
Bme18I GGWCC 6 cut(s) 636, 1567, 1816, 3170, 3696, 3994
BmeT110I CYCGRG 8 cut(s) 770, 1400, 1720, 2333, 3189, 3281, 3565, 3934
BmgBI CACGTC 1 cut(s) 259
BmgT120I GGNCC 8 cut(s) 636, 1191, 1567, 1816, 3170, 3187, 3696, 3994
BmiI GGNNCC 6 cut(s) 436, 2220, 3138, 3591, 3714, 3995
BmrI ACTGGG 1 cut(s) 2873
BmuI ACTGGG 1 cut(s) 2873
BoxI GACNNNNGTC 2 cut(s) 2668, 4065
BpiI GAAGAC 3 cut(s) 504, 1536, 3163
BplI GAGNNNNNCTC 2 cut(s) 515, 547
BpmI CTGGAG 4 cut(s) 453, 828, 1805, 2514
Bpu10I CCTNAGC 5 cut(s) 68, 719, 834, 1832, 3261
BpuEI CTTGAG 5 cut(s) 8, 311, 1261, 2831, 3740
BpuMI CCSGG 8 cut(s) 439, 1592, 1721, 1722, 2683, 2730, 3293, 3594
Bsa29I ATCGAT 1 cut(s) 2889
BsaAI YACGTR 1 cut(s) 1564
BsaBI GATNNNNATC 1 cut(s) 2834
BsaI GGTCTC 1 cut(s) 3186
BsaJI CCNNGG 9 cut(s) 639, 1325, 1720, 1721, 1984, 3132, 3173, 3357, 3430
BsaWI WCCGGW 6 cut(s) 1423, 2665, 3152, 3799, 3996, 4010
Bsc4I CCNNNNNNNGG 9 cut(s) 280, 877, 955, 1727, 2215, 2492, 2498, 3262, 3344
Bse118I RCCGGY 1 cut(s) 3872
Bse1I ACTGG 6 cut(s) 811, 1758, 1822, 1832, 2562, 2868
Bse21I CCTNAGG 2 cut(s) 2930, 3989
Bse3DI GCAATG 3 cut(s) 1877, 2128, 2184
Bse8I GATNNNNATC 1 cut(s) 2834
BseAI TCCGGA 1 cut(s) 3152
BseBI CCWGG 8 cut(s) 122, 245, 353, 1985, 2493, 3133, 3174, 3827
BseCI ATCGAT 1 cut(s) 2889
BseDI CCNNGG 9 cut(s) 639, 1325, 1720, 1721, 1984, 3132, 3173, 3357, 3430
BseGI GGATG 3 cut(s) 52, 203, 2530
BseJI GATNNNNATC 1 cut(s) 2834
BseLI CCNNNNNNNGG 9 cut(s) 280, 877, 955, 1727, 2215, 2492, 2498, 3262, 3344
BseMI GCAATG 3 cut(s) 1877, 2128, 2184
BseMII CTCAG 7 cut(s) 82, 1846, 2921, 3159, 3275, 3980, 4117
BseNI ACTGG 6 cut(s) 811, 1758, 1822, 1832, 2562, 2868
BseRI GAGGAG 5 cut(s) 494, 539, 2287, 3024, 3583
BseYI CCCAGC 3 cut(s) 1679, 2875, 2956
BsgI GTGCAG 4 cut(s) 281, 1886, 3599, 3897
Bsh1236I CGCG 2 cut(s) 983, 1159
BshFI GGCC 5 cut(s) 1192, 2382, 2777, 3010, 3188
BshNI GGYRCC 1 cut(s) 3712
BshVI ATCGAT 1 cut(s) 2889
BsiHKAI GWGCWC 7 cut(s) 531, 769, 2334, 3228, 3453, 3769, 4111
BsiHKCI CYCGRG 8 cut(s) 770, 1400, 1720, 2333, 3189, 3281, 3565, 3934
BslFI GGGAC 5 cut(s) 269, 778, 2084, 2656, 4007
BslI CCNNNNNNNGG 9 cut(s) 280, 877, 955, 1727, 2215, 2492, 2498, 3262, 3344
BsmAI GTCTC 7 cut(s) 1237, 1301, 1849, 1968, 3186, 3405, 4053
BsmBI CGTCTC 1 cut(s) 4053
BsmFI GGGAC 5 cut(s) 269, 778, 2084, 2656, 4007
BsmI GAATGC 3 cut(s) 1237, 3020, 3607
BsnI GGCC 5 cut(s) 1192, 2382, 2777, 3010, 3188
Bso31I GGTCTC 1 cut(s) 3186
BsoBI CYCGRG 8 cut(s) 770, 1400, 1720, 2333, 3189, 3281, 3565, 3934
Bsp1286I GDGCHC 8 cut(s) 531, 769, 2334, 2486, 3228, 3453, 3769, 4111
Bsp13I TCCGGA 1 cut(s) 3152
Bsp19I CCATGG 1 cut(s) 639
BspACI CCGC 9 cut(s) 814, 1159, 1358, 2278, 2367, 3371, 3777, 3890, 4051
BspANI GGCC 5 cut(s) 1192, 2382, 2777, 3010, 3188
BspCNI CTCAG 7 cut(s) 81, 1845, 2922, 3158, 3274, 3981, 4116
BspDI ATCGAT 1 cut(s) 2889
BspEI TCCGGA 1 cut(s) 3152
BspFNI CGCG 2 cut(s) 983, 1159
BspHI TCATGA 3 cut(s) 1705, 3241, 3732
BspLI GGNNCC 6 cut(s) 436, 2220, 3138, 3591, 3714, 3995
BspMAI CTGCAG 8 cut(s) 619, 1296, 1317, 1365, 3106, 3859, 3928, 4088
BspMI ACCTGC 5 cut(s) 1017, 1276, 1306, 1493, 3673
BspPI GGATC 7 cut(s) 36, 2602, 3131, 3144, 3329, 3421, 4088
BspQI GCTCTTC 1 cut(s) 1852
BspT107I GGYRCC 1 cut(s) 3712
BspTI CTTAAG 2 cut(s) 458, 2656
BspTNI GGTCTC 1 cut(s) 3186
BsrDI GCAATG 3 cut(s) 1877, 2128, 2184
BsrFI RCCGGY 1 cut(s) 3872
BsrI ACTGG 6 cut(s) 811, 1758, 1822, 1832, 2562, 2868
BssAI RCCGGY 1 cut(s) 3872
BssECI CCNNGG 9 cut(s) 639, 1325, 1720, 1721, 1984, 3132, 3173, 3357, 3430
BssT1I CCWWGG 4 cut(s) 639, 1325, 3357, 3430
Bst2UI CCWGG 8 cut(s) 122, 245, 353, 1985, 2493, 3133, 3174, 3827
Bst6I CTCTTC 9 cut(s) 112, 371, 944, 1217, 1707, 1806, 1852, 2626, 3510
BstAFI CTTAAG 2 cut(s) 458, 2656
BstAPI GCANNNNNTGC 3 cut(s) 1291, 1876, 3869
BstBAI YACGTR 1 cut(s) 1564
BstDSI CCRYGG 1 cut(s) 639
BstENI CCTNNNNNAGG 2 cut(s) 875, 3260
BstF5I GGATG 3 cut(s) 52, 203, 2530
BstFNI CGCG 2 cut(s) 983, 1159
BstH2I RGCGCY 1 cut(s) 750
BstHHI GCGC 3 cut(s) 457, 521, 749
BstMAI GTCTC 7 cut(s) 1237, 1301, 1849, 1968, 3186, 3405, 4053
BstNI CCWGG 8 cut(s) 122, 245, 353, 1985, 2493, 3133, 3174, 3827
BstNSI RCATGY 7 cut(s) 1450, 1879, 2719, 2904, 3115, 3622, 3650
BstPAI GACNNNNGTC 2 cut(s) 2668, 4065
BstSFI CTRYAG 8 cut(s) 615, 1292, 1313, 1361, 3102, 3855, 3924, 4084
BstUI CGCG 2 cut(s) 983, 1159
BstV2I GAAGAC 3 cut(s) 504, 1536, 3163
BstX2I RGATCY 4 cut(s) 28, 3136, 3426, 4080
BstXI CCANNNNNNTGG 1 cut(s) 2649
BstYI RGATCY 4 cut(s) 28, 3136, 3426, 4080
Bsu15I ATCGAT 1 cut(s) 2889
Bsu36I CCTNAGG 2 cut(s) 2930, 3989
BsuI GTATCC 1 cut(s) 1765
BsuRI GGCC 5 cut(s) 1192, 2382, 2777, 3010, 3188
BsuTUI ATCGAT 1 cut(s) 2889
BtgI CCRYGG 1 cut(s) 639
BtgZI GCGATG 1 cut(s) 3458
BtrI CACGTC 1 cut(s) 259
BtsCI GGATG 3 cut(s) 52, 203, 2530
BtsI GCAGTG 7 cut(s) 594, 1310, 1738, 1869, 1956, 2189, 3885
BveI ACCTGC 5 cut(s) 1017, 1276, 1306, 1493, 3673
CaiI CAGNNNCTG 5 cut(s) 1135, 1291, 3268, 3385, 3869
CciI TCATGA 3 cut(s) 1705, 3241, 3732
CfoI GCGC 3 cut(s) 457, 521, 749
Cfr10I RCCGGY 1 cut(s) 3872
Cfr13I GGNCC 8 cut(s) 636, 1191, 1567, 1816, 3170, 3187, 3696, 3994
Cfr9I CCCGGG 1 cut(s) 1720
ClaI ATCGAT 1 cut(s) 2889
CseI GACGC 2 cut(s) 1165, 1725
CsiI ACCWGGT 1 cut(s) 243
Csp6I GTAC 9 cut(s) 1460, 1561, 1574, 1825, 1898, 2502, 2818, 3718, 4054
CviQI GTAC 9 cut(s) 1460, 1561, 1574, 1825, 1898, 2502, 2818, 3718, 4054
DraI TTTAAA 1 cut(s) 1065
DrdI GACNNNNNNGTC 1 cut(s) 3703
DseDI GACNNNNNNGTC 1 cut(s) 3703
EaeI YGGCCR 2 cut(s) 2380, 3008
Eam1104I CTCTTC 9 cut(s) 112, 371, 944, 1217, 1707, 1806, 1852, 2626, 3510
EarI CTCTTC 9 cut(s) 112, 371, 944, 1217, 1707, 1806, 1852, 2626, 3510
Ecl136II GAGCTC 2 cut(s) 529, 2332
Eco130I CCWWGG 4 cut(s) 639, 1325, 3357, 3430
Eco24I GRGCYC 3 cut(s) 531, 2334, 2486
Eco31I GGTCTC 1 cut(s) 3186
Eco32I GATATC 3 cut(s) 2893, 3239, 3943
Eco47I GGWCC 6 cut(s) 636, 1567, 1816, 3170, 3696, 3994
Eco53kI GAGCTC 2 cut(s) 529, 2332
Eco57I CTGAAG 3 cut(s) 581, 2568, 3407
Eco81I CCTNAGG 2 cut(s) 2930, 3989
Eco88I CYCGRG 8 cut(s) 770, 1400, 1720, 2333, 3189, 3281, 3565, 3934
EcoICRI GAGCTC 2 cut(s) 529, 2332
EcoNI CCTNNNNNAGG 2 cut(s) 875, 3260
EcoO109I RGGNCCY 2 cut(s) 1816, 3170
EcoRI GAATTC 3 cut(s) 586, 1366, 1691
EcoRII CCWGG 8 cut(s) 120, 243, 351, 1983, 2491, 3131, 3172, 3825
EcoRV GATATC 3 cut(s) 2893, 3239, 3943
EcoT14I CCWWGG 4 cut(s) 639, 1325, 3357, 3430
EcoT22I ATGCAT 2 cut(s) 1877, 3648
EcoT38I GRGCYC 3 cut(s) 531, 2334, 2486
ErhI CCWWGG 4 cut(s) 639, 1325, 3357, 3430
Esp3I CGTCTC 1 cut(s) 4053
FalI AAGNNNNNCTT 1 cut(s) 4116
FaqI GGGAC 5 cut(s) 269, 778, 2084, 2656, 4007
FauI CCCGC 1 cut(s) 2271
FbaI TGATCA 2 cut(s) 390, 3576
FokI GGATG 3 cut(s) 59, 190, 2537
FriOI GRGCYC 3 cut(s) 531, 2334, 2486
FspBI CTAG 4 cut(s) 1854, 2396, 3167, 3773
GlaI GCGC 3 cut(s) 456, 520, 748
GsaI CCCAGC 3 cut(s) 1683, 2879, 2960
GsuI CTGGAG 4 cut(s) 453, 828, 1805, 2514
HaeII RGCGCY 1 cut(s) 750
HaeIII GGCC 5 cut(s) 1192, 2382, 2777, 3010, 3188
HgaI GACGC 2 cut(s) 1165, 1725
HhaI GCGC 3 cut(s) 457, 521, 749
Hin6I GCGC 3 cut(s) 455, 519, 747
HinP1I GCGC 3 cut(s) 455, 519, 747
HincII GTYRAC 4 cut(s) 142, 570, 1996, 3688
HindII GTYRAC 4 cut(s) 142, 570, 1996, 3688
HindIII AAGCTT 3 cut(s) 1219, 1553, 2473
HphI GGTGA 6 cut(s) 233, 892, 2054, 2291, 2977, 3571
Hpy99I CGWCG 1 cut(s) 3639
HpyCH4IV ACGT 3 cut(s) 150, 258, 1563
HpySE526I ACGT 3 cut(s) 150, 258, 1563
HspAI GCGC 3 cut(s) 455, 519, 747
Kpn2I TCCGGA 1 cut(s) 3152
Ksp22I TGATCA 2 cut(s) 390, 3576
LguI GCTCTTC 1 cut(s) 1852
LmnI GCTCC 8 cut(s) 434, 526, 2795, 2909, 3231, 3764, 3851, 3884
MabI ACCWGGT 1 cut(s) 243
MaeI CTAG 4 cut(s) 1854, 2396, 3167, 3773
MaeII ACGT 3 cut(s) 150, 258, 1563
MaeIII GTNAC 8 cut(s) 1072, 1254, 1888, 1929, 2879, 2975, 3314, 3493
MflI RGATCY 4 cut(s) 28, 3136, 3426, 4080
MhlI GDGCHC 8 cut(s) 531, 769, 2334, 2486, 3228, 3453, 3769, 4111
MlsI TGGCCA 1 cut(s) 2382
MluI ACGCGT 1 cut(s) 981
MluNI TGGCCA 1 cut(s) 2382
MlyI GAGTC 9 cut(s) 494, 575, 930, 1392, 2106, 2241, 2678, 3143, 3450
MmeI TCCRAC 3 cut(s) 1597, 2442, 3362
Mox20I TGGCCA 1 cut(s) 2382
Mph1103I ATGCAT 2 cut(s) 1877, 3648
MroI TCCGGA 1 cut(s) 3152
MroXI GAANNNNTTC 2 cut(s) 2192, 3536
MscI TGGCCA 1 cut(s) 2382
MslI CAYNNNNRTG 4 cut(s) 1731, 2647, 3584, 3970
Msp20I TGGCCA 1 cut(s) 2382
MspA1I CMGCKG 4 cut(s) 932, 1360, 2116, 2875
MspCI CTTAAG 2 cut(s) 458, 2656
Mva1269I GAATGC 3 cut(s) 1237, 3020, 3607
MvaI CCWGG 8 cut(s) 122, 245, 353, 1985, 2493, 3133, 3174, 3827
MvnI CGCG 2 cut(s) 983, 1159
NciI CCSGG 8 cut(s) 439, 1592, 1721, 1722, 2683, 2730, 3293, 3594
NcoI CCATGG 1 cut(s) 639
NlaIV GGNNCC 6 cut(s) 436, 2220, 3138, 3591, 3714, 3995
NmeAIII GCCGAG 1 cut(s) 4051
NmuCI GTSAC 4 cut(s) 1888, 1929, 3314, 3493
NsiI ATGCAT 2 cut(s) 1877, 3648
NspI RCATGY 7 cut(s) 1450, 1879, 2719, 2904, 3115, 3622, 3650
PaeI GCATGC 2 cut(s) 1879, 2904
PaeR7I CTCGAG 2 cut(s) 3565, 3934
PagI TCATGA 3 cut(s) 1705, 3241, 3732
PciI ACATGT 2 cut(s) 2715, 3618
PciSI GCTCTTC 1 cut(s) 1852
PctI GAATGC 3 cut(s) 1237, 3020, 3607
PdmI GAANNNNTTC 2 cut(s) 2192, 3536
PflMI CCANNNNNTGG 1 cut(s) 955
PfoI TCCNGGA 2 cut(s) 120, 2491
PleI GAGTC 9 cut(s) 494, 575, 930, 1392, 2105, 2240, 2677, 3143, 3450
PpsI GAGTC 9 cut(s) 494, 575, 930, 1392, 2105, 2240, 2677, 3143, 3450
Ppu21I YACGTR 1 cut(s) 1564
PpuMI RGGWCCY 2 cut(s) 1816, 3170
PscI ACATGT 2 cut(s) 2715, 3618
PshAI GACNNNNGTC 2 cut(s) 2668, 4065
PsiI TTATAA 1 cut(s) 2951
Psp124BI GAGCTC 2 cut(s) 531, 2334
Psp1406I AACGTT 1 cut(s) 150
Psp5II RGGWCCY 2 cut(s) 1816, 3170
Psp6I CCWGG 8 cut(s) 120, 243, 351, 1983, 2491, 3131, 3172, 3825
PspFI CCCAGC 3 cut(s) 1679, 2875, 2956
PspGI CCWGG 8 cut(s) 120, 243, 351, 1983, 2491, 3131, 3172, 3825
PspN4I GGNNCC 6 cut(s) 436, 2220, 3138, 3591, 3714, 3995
PspPI GGNCC 8 cut(s) 636, 1191, 1567, 1816, 3170, 3187, 3696, 3994
PspPPI RGGWCCY 2 cut(s) 1816, 3170
PspXI VCTCGAGB 1 cut(s) 3565
PstI CTGCAG 8 cut(s) 619, 1296, 1317, 1365, 3106, 3859, 3928, 4088
PstNI CAGNNNCTG 5 cut(s) 1135, 1291, 3268, 3385, 3869
PsuI RGATCY 4 cut(s) 28, 3136, 3426, 4080
PvuII CAGCTG 3 cut(s) 932, 2116, 2875
RsaI GTAC 9 cut(s) 1461, 1562, 1575, 1826, 1899, 2503, 2819, 3719, 4055
RsaNI GTAC 9 cut(s) 1460, 1561, 1574, 1825, 1898, 2502, 2818, 3718, 4054
RseI CAYNNNNRTG 4 cut(s) 1731, 2647, 3584, 3970
SacI GAGCTC 2 cut(s) 531, 2334
SapI GCTCTTC 1 cut(s) 1852
Sau96I GGNCC 8 cut(s) 636, 1191, 1567, 1816, 3170, 3187, 3696, 3994
ScaI AGTACT 2 cut(s) 1575, 1826
SchI GAGTC 9 cut(s) 494, 575, 930, 1392, 2106, 2241, 2678, 3143, 3450
SduI GDGCHC 8 cut(s) 531, 769, 2334, 2486, 3228, 3453, 3769, 4111
SexAI ACCWGGT 1 cut(s) 243
SfcI CTRYAG 8 cut(s) 615, 1292, 1313, 1361, 3102, 3855, 3924, 4084
Sfr274I CTCGAG 2 cut(s) 3565, 3934
SinI GGWCC 6 cut(s) 636, 1567, 1816, 3170, 3696, 3994
SlaI CTCGAG 2 cut(s) 3565, 3934
SmaI CCCGGG 1 cut(s) 1722
SmiMI CAYNNNNRTG 4 cut(s) 1731, 2647, 3584, 3970
SmlI CTYRAG 9 cut(s) 23, 290, 458, 1240, 2656, 2810, 3565, 3755, 3934
SmoI CTYRAG 9 cut(s) 23, 290, 458, 1240, 2656, 2810, 3565, 3755, 3934
SphI GCATGC 2 cut(s) 1879, 2904
SsiI CCGC 9 cut(s) 814, 1159, 1358, 2278, 2367, 3371, 3777, 3890, 4051
SspI AATATT 3 cut(s) 576, 1339, 2139
SspMI CTAG 4 cut(s) 1854, 2396, 3167, 3773
SstI GAGCTC 2 cut(s) 531, 2334
StyI CCWWGG 4 cut(s) 639, 1325, 3357, 3430
TaiI ACGT 3 cut(s) 153, 261, 1566
TatI WGTACW 3 cut(s) 1573, 1824, 1897
TauI GCSGC 4 cut(s) 1360, 2370, 3779, 3893
TseFI GTSAC 4 cut(s) 1888, 1929, 3314, 3493
Tsp45I GTSAC 4 cut(s) 1888, 1929, 3314, 3493
TspGWI ACGGA 4 cut(s) 127, 684, 2808, 3206
TspMI CCCGGG 1 cut(s) 1720
Van91I CCANNNNNTGG 1 cut(s) 955
Vha464I CTTAAG 2 cut(s) 458, 2656
VpaK11BI GGWCC 6 cut(s) 636, 1567, 1816, 3170, 3696, 3994
XagI CCTNNNNNAGG 2 cut(s) 875, 3260
XapI RAATTY 5 cut(s) 586, 1366, 1691, 3162, 3629
XbaI TCTAGA 1 cut(s) 1853
XceI RCATGY 7 cut(s) 1450, 1879, 2719, 2904, 3115, 3622, 3650
XcmI CCANNNNNNNNNTGG 2 cut(s) 656, 2181
XhoI CTCGAG 2 cut(s) 3565, 3934
XmaI CCCGGG 1 cut(s) 1720
XmnI GAANNNNTTC 2 cut(s) 2192, 3536
XspI CTAG 4 cut(s) 1854, 2396, 3167, 3773
ZrmI AGTACT 2 cut(s) 1575, 1826
Zsp2I ATGCAT 2 cut(s) 1877, 3648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.