MD03G1194000.v1.1
ERF Family

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin Fzo YdjA family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007671
Physical Location & Seq
Reverse (-)
26619866 .. 26625070
5205 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1194000.v1.1.491

Sequence Viewer

Length: 1674 bp
ATGGAAATTTCTTCAGTTCCGATCAGTTCGTGCTCGAAAGAGCATCAGAATATCTACCAGGAATGGTTTCGATTCGCTGATTCAGACACTGATGGCCGCATTACCGGGAGTGATGCTATAAAGTTCTTCGGCATGTCCAATCTGAATCGGCAGGATCTCAAGCAGGTTTGGGCCATGGCCGATTCAAAGAGGCAGGGATATCTTGGTTTCAGTGAGTTCGTTGCCGCTATGCAGCTAGTTTCTCTTGGTCAAGCTGGACATGATATTACACACGGTTTATTGAATAGCAATGTTGACTTGGAAAGTCTGAAACCTCCTGTTATGGAGGGTTTGGATGCATTACTATTAGTGAAGAAAAAGCATTCGCACAAGTCAAATGAAAACGAAGTAAATGGAACTGCTGTGGTTAAACAATCACCTTCGGCACTTTGGTTTTCATCAAAATCATCAAAAAAGGTACCACTTTCTTCTGTAACATCAATTATTGATGGGTTGAAGAGACTGTATGTTCAGAAGCTGAAGCCATTAGAAGTTACATACAAGTTCAACGATTTTGTATCCCCATTACTGACAAATAGTGATTTTGATGCCAAACCCATGGTTATGCTTTTGGGTCAATACTCCACTGGGAAAACAACATTCATTAAACATTTGCTTAAAAGTAGTTATCCAGGCAAGTTATCCTCTGGGAAAGTGTCTTTGTGTAGAGCTCACATTGGTCCCGAGCCTACAACCGATAGATTTGTTGTTGTTATGTCTGGGCCTGATGAAAGAAGTGTTCCTGGGAATACTATTGCCGTCCAAGCTGACATGCCATTTAGCGGTCTCACAACTTTTGGAACAGCATTCTTGTCAAAGTTTGAGTGTTCTCAAATGCCACATTCACTGCTAGAACACATTACTTTTGTGGATACCCCTGGAGTTTTATCTGGAGAGAAGCAACGGACACATCGAGCCTACGATTTTACTGGGGTAACTTCGTGGTTTGCTGCAAAGTGTGACCTCATTCTACTTCTGTTTGATCCTCACAAACTCGATGTTAGTGATGAGTTCAAGCGTGTTATTTCATCTTTACATGGCCATGATGATAAAATTCGTGTTGTTCTGAACAAGGCGGATCAAATTGATACTCAGCAATTGATGAGAGTTTATGGAGCATTGATGTGGTCACTTGGGAAGGTTCTTAATACTCCTGAGGTCATGCGAGTTTATATCGGCTCATTCAATGACAAACCTGTAAATGAGGCTGCTACGGGTCCAGTTGGGAAAGAACTCTTTGAAAGGGAACAAGAGGATCTTCTTGCTGATTTAAAGGATATTCCAAAGAAGGCCTGTGATCGCAGAATCAATGAATTTGTGAAGCGTGCCAGAGCTGCCAAGATACATGCTTACATAATTAGCCATTTAAGGAAGGAGATGCCTGCCATGTTGGGGAAATCTAAGGCTCAACGGAAGCTTATTGATAATTTGGAAGATGAATTTAAAAAGGTCCAGAGGGAGCACCATCTACCTCCAGGGGATTTCCCGAACGTAGACCACTACAGGGACATCTTGACTGGTTACAGCTTTGACAAATTGGAGAGGTTAAAGCCTAAGATGATAGATGCCGTCGATGAAATGCTGGGTTATGATATCCCGGAACTCTTGAAGAAATTCAGAAATCCATACGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005886 GO:0005911 GO:0005929 GO:0006810 GO:0006886 GO:0006897 GO:0006950 GO:0006970 GO:0006972 GO:0006996 GO:0007275 GO:0007399 GO:0008104 GO:0008150 GO:0009506 GO:0009628 GO:0009651 GO:0009719 GO:0009987 GO:0010008 GO:0010033 GO:0010830 GO:0010831 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0020016 GO:0020018 GO:0022008 GO:0022603 GO:0022607 GO:0030030 GO:0030031 GO:0030054 GO:0030154 GO:0030182 GO:0031090 GO:0031175 GO:0031253 GO:0031410 GO:0031901 GO:0031982 GO:0032386 GO:0032388 GO:0032456 GO:0032501 GO:0032502 GO:0032879 GO:0033036 GO:0033365 GO:0034613 GO:0042221 GO:0042538 GO:0042886 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044441 GO:0044444 GO:0044446 GO:0044459 GO:0044463 GO:0044464 GO:0044782 GO:0045184 GO:0045595 GO:0045597 GO:0046907 GO:0048468 GO:0048518 GO:0048522 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051128 GO:0051130 GO:0051147 GO:0051149 GO:0051153 GO:0051155 GO:0051179 GO:0051234 GO:0051259 GO:0051260 GO:0051641 GO:0051649 GO:0051716 GO:0055037 GO:0055038 GO:0055044 GO:0060142 GO:0060143 GO:0060170 GO:0060271 GO:0060341 GO:0060627 GO:0061512 GO:0065003 GO:0065007 GO:0070727 GO:0070848 GO:0070887 GO:0070925 GO:0071310 GO:0071363 GO:0071495 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0097708 GO:0098588 GO:0098590 GO:0098657 GO:0098805 GO:0120025 GO:0120031 GO:0120036 GO:0120038 GO:1901739 GO:1901741 GO:1990089 GO:1990090 GO:2001135 GO:2001137
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Dynamin_N EF-hand_4 EHD_N

Protein Analysis

558

Amino Acids

62.7

Weight (kDa)

8.49

Isoelectric Point (pI)

32.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EH PF12763 14 - 86 7.9e-12 EH domain
EF-hand_7 PF13499 22 - 78 2e-06 EF-hand domain pair
EHD_N PF16880 164 - 196 3.4e-16 N-terminal EH-domain containing protein
Dynamin_N PF00350 201 - 372 2.3e-10 Dynamin family
DUF5600 PF18150 447 - 548 4e-38 Domain of unknown function (DUF5600)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 154
Acc65I GGTACC 1 cut(s) 457
AccB1I GGYRCC 1 cut(s) 457
AccI GTMKAC 1 cut(s) 1533
AciI CCGC 4 cut(s) 97, 225, 822, 1115
AclWI GGATC 4 cut(s) 162, 1016, 1125, 1302
AcoI YGGCCR 3 cut(s) 94, 177, 1078
AcsI RAATTY 5 cut(s) 6, 1092, 1352, 1478, 1652
AcuI CTGAAG 1 cut(s) 539
AfaI GTAC 1 cut(s) 459
AfiI CCNNNNNNNGG 3 cut(s) 821, 1431, 1543
AgsI TTSAA 8 cut(s) 186, 283, 496, 547, 1054, 1225, 1280, 1648
AjnI CCWGG 5 cut(s) 57, 670, 781, 916, 1513
AluBI AGCT 8 cut(s) 235, 254, 517, 710, 806, 1373, 1456, 1566
AluI AGCT 8 cut(s) 235, 254, 517, 710, 806, 1373, 1456, 1566
Alw21I GWGCWC 3 cut(s) 35, 712, 1503
Alw26I GTCTC 2 cut(s) 493, 830
AlwI GGATC 4 cut(s) 162, 1016, 1125, 1302
AlwNI CAGNNNCTG 2 cut(s) 89, 517
Ama87I CYCGRG 1 cut(s) 722
AoxI GGCC 6 cut(s) 94, 171, 177, 761, 1078, 1329
ApeKI GCWGC 4 cut(s) 232, 989, 1247, 1373
ApoI RAATTY 5 cut(s) 6, 1092, 1352, 1478, 1652
Asp700I GAANNNNTTC 2 cut(s) 66, 1652
Asp718I GGTACC 1 cut(s) 457
AspS9I GGNCC 5 cut(s) 171, 719, 761, 1256, 1489
AsuC2I CCSGG 2 cut(s) 106, 1637
AsuHPI GGTGA 1 cut(s) 408
AvaI CYCGRG 1 cut(s) 722
AvaII GGWCC 3 cut(s) 719, 1256, 1489
AxyI CCTNAGG 1 cut(s) 1194
BalI TGGCCA 1 cut(s) 1080
BanI GGYRCC 1 cut(s) 457
BanII GRGCYC 1 cut(s) 712
Bbv12I GWGCWC 3 cut(s) 35, 712, 1503
BbvI GCAGC 4 cut(s) 244, 976, 1234, 1360
BccI CCATC 3 cut(s) 86, 482, 1512
BceAI ACGGC 2 cut(s) 782, 1592
BciT130I CCWGG 5 cut(s) 59, 672, 783, 918, 1515
BciVI GTATCC 2 cut(s) 568, 904
BcnI CCSGG 2 cut(s) 106, 1637
BcoDI GTCTC 2 cut(s) 493, 830
BfaI CTAG 2 cut(s) 236, 890
BfmI CTRYAG 1 cut(s) 1540
BfuAI ACCTGC 1 cut(s) 154
BfuI GTATCC 2 cut(s) 568, 904
BisI GCNGC 6 cut(s) 97, 225, 233, 990, 1248, 1374
BlsI GCNGC 6 cut(s) 98, 226, 234, 991, 1249, 1375
Bme1390I CCNGG 7 cut(s) 59, 106, 672, 783, 918, 1515, 1637
Bme18I GGWCC 3 cut(s) 719, 1256, 1489
BmeT110I CYCGRG 1 cut(s) 722
BmgT120I GGNCC 5 cut(s) 171, 719, 761, 1256, 1489
BmiI GGNNCC 3 cut(s) 459, 721, 1257
BmrFI CCNGG 7 cut(s) 59, 106, 672, 783, 918, 1515, 1637
BmrI ACTGGG 2 cut(s) 636, 978
BmsI GCATC 6 cut(s) 52, 103, 325, 577, 1407, 1594
BmuI ACTGGG 2 cut(s) 636, 978
BpmI CTGGAG 3 cut(s) 939, 951, 1497
BpuEI CTTGAG 1 cut(s) 143
BpuMI CCSGG 2 cut(s) 106, 1637
BsaI GGTCTC 1 cut(s) 830
BsaJI CCNNGG 5 cut(s) 174, 597, 782, 916, 1514
Bsc4I CCNNNNNNNGG 3 cut(s) 821, 1431, 1543
Bse1I ACTGG 4 cut(s) 631, 973, 1259, 1561
Bse21I CCTNAGG 1 cut(s) 1194
Bse3DI GCAATG 1 cut(s) 295
BseBI CCWGG 5 cut(s) 59, 672, 783, 918, 1515
BseDI CCNNGG 5 cut(s) 174, 597, 782, 916, 1514
BseGI GGATG 1 cut(s) 340
BseLI CCNNNNNNNGG 3 cut(s) 821, 1431, 1543
BseMI GCAATG 1 cut(s) 295
BseMII CTCAG 2 cut(s) 1145, 1185
BseNI ACTGG 4 cut(s) 631, 973, 1259, 1561
BseXI GCAGC 4 cut(s) 244, 976, 1234, 1360
BseYI CCCAGC 1 cut(s) 1621
BshFI GGCC 6 cut(s) 96, 173, 179, 763, 1080, 1331
BshNI GGYRCC 1 cut(s) 457
BsiHKAI GWGCWC 3 cut(s) 35, 712, 1503
BsiHKCI CYCGRG 1 cut(s) 722
BsiSI CCGG 2 cut(s) 105, 1637
BslFI GGGAC 2 cut(s) 705, 1559
BslI CCNNNNNNNGG 3 cut(s) 821, 1431, 1543
BsmAI GTCTC 2 cut(s) 493, 830
BsmFI GGGAC 2 cut(s) 705, 1559
BsmI GAATGC 2 cut(s) 361, 845
BsnI GGCC 6 cut(s) 96, 173, 179, 763, 1080, 1331
Bso31I GGTCTC 1 cut(s) 830
BsoBI CYCGRG 1 cut(s) 722
Bsp1286I GDGCHC 3 cut(s) 35, 712, 1503
Bsp143I GATC 6 cut(s) 21, 154, 1021, 1117, 1294, 1336
Bsp19I CCATGG 2 cut(s) 174, 597
BspACI CCGC 4 cut(s) 97, 225, 822, 1115
BspANI GGCC 6 cut(s) 96, 173, 179, 763, 1080, 1331
BspCNI CTCAG 2 cut(s) 1144, 1186
BspLI GGNNCC 3 cut(s) 459, 721, 1257
BspMI ACCTGC 1 cut(s) 154
BspPI GGATC 4 cut(s) 162, 1016, 1125, 1302
BspT107I GGYRCC 1 cut(s) 457
BspTNI GGTCTC 1 cut(s) 830
BsrDI GCAATG 1 cut(s) 295
BsrI ACTGG 4 cut(s) 631, 973, 1259, 1561
BssECI CCNNGG 5 cut(s) 174, 597, 782, 916, 1514
BssMI GATC 6 cut(s) 21, 154, 1021, 1117, 1294, 1336
BssT1I CCWWGG 2 cut(s) 174, 597
Bst2UI CCWGG 5 cut(s) 59, 672, 783, 918, 1515
Bst4CI ACNGT 2 cut(s) 275, 504
Bst6I CTCTTC 1 cut(s) 491
BstC8I GCNNGC 2 cut(s) 1365, 1422
BstDEI CTNAG 4 cut(s) 1131, 1194, 1440, 1593
BstDSI CCRYGG 2 cut(s) 174, 597
BstF5I GGATG 1 cut(s) 340
BstKTI GATC 6 cut(s) 24, 157, 1024, 1120, 1297, 1339
BstMAI GTCTC 2 cut(s) 493, 830
BstMBI GATC 6 cut(s) 21, 154, 1021, 1117, 1294, 1336
BstMWI GCNNNNNNNGC 2 cut(s) 803, 1373
BstNI CCWGG 5 cut(s) 59, 672, 783, 918, 1515
BstNSI RCATGY 3 cut(s) 136, 814, 1388
BstSCI CCNGG 7 cut(s) 57, 104, 670, 781, 916, 1513, 1635
BstSFI CTRYAG 1 cut(s) 1540
BstV1I GCAGC 4 cut(s) 244, 976, 1234, 1360
BstX2I RGATCY 2 cut(s) 154, 1294
BstXI CCANNNNNNTGG 1 cut(s) 598
BstYI RGATCY 2 cut(s) 154, 1294
Bsu36I CCTNAGG 1 cut(s) 1194
BsuI GTATCC 2 cut(s) 568, 904
BsuRI GGCC 6 cut(s) 96, 173, 179, 763, 1080, 1331
BtgI CCRYGG 2 cut(s) 174, 597
BtsCI GGATG 1 cut(s) 340
BtsI GCAGTG 1 cut(s) 884
BtsIMutI CAGTG 4 cut(s) 87, 217, 624, 884
BveI ACCTGC 1 cut(s) 154
Cac8I GCNNGC 2 cut(s) 1365, 1422
CaiI CAGNNNCTG 2 cut(s) 89, 517
Cfr13I GGNCC 5 cut(s) 171, 719, 761, 1256, 1489
Csp6I GTAC 1 cut(s) 458
CviQI GTAC 1 cut(s) 458
DdeI CTNAG 4 cut(s) 1131, 1194, 1440, 1593
DpnI GATC 6 cut(s) 23, 156, 1023, 1119, 1296, 1338
DpnII GATC 6 cut(s) 21, 154, 1021, 1117, 1294, 1336
DraI TTTAAA 2 cut(s) 1311, 1483
EaeI YGGCCR 3 cut(s) 94, 177, 1078
Eam1104I CTCTTC 1 cut(s) 491
EarI CTCTTC 1 cut(s) 491
EciI GGCGGA 1 cut(s) 1130
Ecl136II GAGCTC 1 cut(s) 710
Eco130I CCWWGG 2 cut(s) 174, 597
Eco147I AGGCCT 1 cut(s) 1331
Eco24I GRGCYC 1 cut(s) 712
Eco31I GGTCTC 1 cut(s) 830
Eco32I GATATC 2 cut(s) 200, 1633
Eco47I GGWCC 3 cut(s) 719, 1256, 1489
Eco53kI GAGCTC 1 cut(s) 710
Eco57I CTGAAG 1 cut(s) 539
Eco81I CCTNAGG 1 cut(s) 1194
Eco88I CYCGRG 1 cut(s) 722
EcoICRI GAGCTC 1 cut(s) 710
EcoRII CCWGG 5 cut(s) 57, 670, 781, 916, 1513
EcoRV GATATC 2 cut(s) 200, 1633
EcoT14I CCWWGG 2 cut(s) 174, 597
EcoT22I ATGCAT 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 712
ErhI CCWWGG 2 cut(s) 174, 597
FalI AAGNNNNNCTT 2 cut(s) 1281, 1313
FaqI GGGAC 2 cut(s) 705, 1559
FblI GTMKAC 1 cut(s) 1533
Fnu4HI GCNGC 6 cut(s) 97, 225, 233, 990, 1248, 1374
FokI GGATG 1 cut(s) 347
FriOI GRGCYC 1 cut(s) 712
Fsp4HI GCNGC 6 cut(s) 97, 225, 233, 990, 1248, 1374
FspBI CTAG 2 cut(s) 236, 890
GluI GCNGC 6 cut(s) 97, 225, 233, 990, 1248, 1374
GsaI CCCAGC 1 cut(s) 1625
GsuI CTGGAG 3 cut(s) 939, 951, 1497
HaeIII GGCC 6 cut(s) 96, 173, 179, 763, 1080, 1331
HapII CCGG 2 cut(s) 105, 1637
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HindIII AAGCTT 1 cut(s) 1454
HinfI GANTC 5 cut(s) 72, 80, 145, 182, 1344
HpaII CCGG 2 cut(s) 105, 1637
HphI GGTGA 1 cut(s) 408
Hpy166II GTNNAC 2 cut(s) 295, 1534
Hpy188I TCNGA 8 cut(s) 21, 48, 85, 144, 309, 513, 1107, 1658
Hpy188III TCNNGA 7 cut(s) 722, 930, 1193, 1492, 1525, 1552, 1645
Hpy8I GTNNAC 2 cut(s) 295, 1534
Hpy99I CGWCG 1 cut(s) 1613
HpyAV CCTTC 4 cut(s) 429, 1171, 1321, 1405
HpyCH4III ACNGT 2 cut(s) 275, 504
HpyCH4IV ACGT 1 cut(s) 1530
HpyCH4V TGCA 3 cut(s) 232, 338, 992
HpyF10VI GCNNNNNNNGC 2 cut(s) 803, 1373
HpyF3I CTNAG 4 cut(s) 1131, 1194, 1440, 1593
HpySE526I ACGT 1 cut(s) 1530
KpnI GGTACC 1 cut(s) 461
Kzo9I GATC 6 cut(s) 21, 154, 1021, 1117, 1294, 1336
LmnI GCTCC 2 cut(s) 1154, 1498
Lsp1109I GCAGC 4 cut(s) 244, 976, 1234, 1360
LweI GCATC 6 cut(s) 52, 103, 325, 577, 1407, 1594
MaeI CTAG 2 cut(s) 236, 890
MaeII ACGT 1 cut(s) 1530
MaeIII GTNAC 6 cut(s) 472, 532, 973, 998, 1167, 1559
MalI GATC 6 cut(s) 23, 156, 1023, 1119, 1296, 1338
MboI GATC 6 cut(s) 21, 154, 1021, 1117, 1294, 1336
MboII GAAGA 8 cut(s) 3, 118, 364, 459, 508, 1289, 1484, 1660
MfeI CAATTG 1 cut(s) 1136
MflI RGATCY 2 cut(s) 154, 1294
MhlI GDGCHC 3 cut(s) 35, 712, 1503
MlsI TGGCCA 1 cut(s) 1080
MluNI TGGCCA 1 cut(s) 1080
Mox20I TGGCCA 1 cut(s) 1080
Mph1103I ATGCAT 1 cut(s) 340
MroXI GAANNNNTTC 2 cut(s) 66, 1652
MscI TGGCCA 1 cut(s) 1080
MseI TTAA 8 cut(s) 408, 645, 657, 1185, 1310, 1406, 1482, 1586
MslI CAYNNNNRTG 3 cut(s) 602, 1080, 1162
Msp20I TGGCCA 1 cut(s) 1080
MspI CCGG 2 cut(s) 105, 1637
MspR9I CCNGG 7 cut(s) 59, 106, 672, 783, 918, 1515, 1637
MunI CAATTG 1 cut(s) 1136
Mva1269I GAATGC 2 cut(s) 361, 845
MvaI CCWGG 5 cut(s) 59, 672, 783, 918, 1515
MwoI GCNNNNNNNGC 2 cut(s) 803, 1373
NciI CCSGG 2 cut(s) 106, 1637
NcoI CCATGG 2 cut(s) 174, 597
NdeII GATC 6 cut(s) 21, 154, 1021, 1117, 1294, 1336
NlaIV GGNNCC 3 cut(s) 459, 721, 1257
NmuCI GTSAC 2 cut(s) 998, 1167
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 3 cut(s) 136, 814, 1388
PceI AGGCCT 1 cut(s) 1331
PcsI WCGNNNNNNNCGW 1 cut(s) 949
PctI GAATGC 2 cut(s) 361, 845
PdmI GAANNNNTTC 2 cut(s) 66, 1652
PfeI GAWTC 5 cut(s) 72, 80, 145, 182, 1344
PfoI TCCNGGA 1 cut(s) 1635
PkrI GCNGC 6 cut(s) 98, 226, 234, 991, 1249, 1375
Psp124BI GAGCTC 1 cut(s) 712
Psp6I CCWGG 5 cut(s) 57, 670, 781, 916, 1513
PspFI CCCAGC 1 cut(s) 1621
PspGI CCWGG 5 cut(s) 57, 670, 781, 916, 1513
PspN4I GGNNCC 3 cut(s) 459, 721, 1257
PspPI GGNCC 5 cut(s) 171, 719, 761, 1256, 1489
PstNI CAGNNNCTG 2 cut(s) 89, 517
PsuI RGATCY 2 cut(s) 154, 1294
RsaI GTAC 1 cut(s) 459
RsaNI GTAC 1 cut(s) 458
RseI CAYNNNNRTG 3 cut(s) 602, 1080, 1162
SacI GAGCTC 1 cut(s) 712
SaqAI TTAA 8 cut(s) 408, 645, 657, 1185, 1310, 1406, 1482, 1586
SatI GCNGC 6 cut(s) 97, 225, 233, 990, 1248, 1374
Sau3AI GATC 6 cut(s) 21, 154, 1021, 1117, 1294, 1336
Sau96I GGNCC 5 cut(s) 171, 719, 761, 1256, 1489
ScrFI CCNGG 7 cut(s) 59, 106, 672, 783, 918, 1515, 1637
SduI GDGCHC 3 cut(s) 35, 712, 1503
SfaNI GCATC 6 cut(s) 52, 103, 325, 577, 1407, 1594
SfcI CTRYAG 1 cut(s) 1540
SinI GGWCC 3 cut(s) 719, 1256, 1489
SmiMI CAYNNNNRTG 3 cut(s) 602, 1080, 1162
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
SseBI AGGCCT 1 cut(s) 1331
SsiI CCGC 4 cut(s) 97, 225, 822, 1115
SspMI CTAG 2 cut(s) 236, 890
SstI GAGCTC 1 cut(s) 712
StuI AGGCCT 1 cut(s) 1331
StyD4I CCNGG 7 cut(s) 57, 104, 670, 781, 916, 1513, 1635
StyI CCWWGG 2 cut(s) 174, 597
TaaI ACNGT 2 cut(s) 275, 504
TaiI ACGT 1 cut(s) 1533
TaqI TCGA 5 cut(s) 35, 70, 952, 1035, 1611
TauI GCSGC 2 cut(s) 99, 227
TfiI GAWTC 5 cut(s) 72, 80, 145, 182, 1344
Tru1I TTAA 8 cut(s) 408, 645, 657, 1185, 1310, 1406, 1482, 1586
Tru9I TTAA 8 cut(s) 408, 645, 657, 1185, 1310, 1406, 1482, 1586
TscAI CASTG 4 cut(s) 94, 217, 631, 891
TseFI GTSAC 2 cut(s) 998, 1167
TseI GCWGC 4 cut(s) 232, 989, 1247, 1373
Tsp45I GTSAC 2 cut(s) 998, 1167
TspDTI ATGAA 8 cut(s) 393, 426, 631, 783, 1056, 1365, 1491, 1629
TspGWI ACGGA 2 cut(s) 958, 1465
TspRI CASTG 4 cut(s) 94, 217, 631, 891
VpaK11BI GGWCC 3 cut(s) 719, 1256, 1489
XapI RAATTY 5 cut(s) 6, 1092, 1352, 1478, 1652
XceI RCATGY 3 cut(s) 136, 814, 1388
XmiI GTMKAC 1 cut(s) 1533
XmnI GAANNNNTTC 2 cut(s) 66, 1652
XspI CTAG 2 cut(s) 236, 890
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.