Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0006074

Physical Location & Seq

Genomic Coordinates Reverse (-)

28709817 .. 28711757

Gene Structure 1941 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1208000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1275 bp

ATGAAACTCTCCTTCTCTCTCCCTCCCAAATCATCCTCCAAATCAAACCCTAAACGCAACCCCTCCTCCGCCACCTCCACCTTCAACGACGGCACCGGAAACCACCCTAATGACGCCGTTTCCAAACACTTCGTCAGCGAATTCGACGCCTCGAAGCCCTTCTCCGCCGACCTCAAAGCCCGCGTGATCGCCCCGATCCCCAACGAATGGCGGCCCCACAAGAAGATGAAGAACATCGAGCTTCCCATCACCGAGTCCGGCGGCCACGAGCTCCAGTTCGAGGTCGAGACGCTCTCCGTCACCGACGACCCCGACGCCAAGATTTCGTACGGTCTTAATCTCCGGGAGAAATCGGAATTGGAGAATCGGGAGGGGTTGGGGAATGGTGACCGGCCGAGATTGAGAGGCGTGGAGGATACTCTGCTGCAGAAGTTTAAAGACGATTTGGAGCGCTTGTCGGATCATAGGGGGTTGGAGGAGTTTGAAGAGATGCCGGTGGAGGGTTACGGCGAGGCGTTGCTTACCGGGTACGGGTGGTACCCGGGTAGAGGAATTGGGAAGAATGCGAAGGAAGATGTGAAGATAGTGGAGTATACTAGGAGTACTGATAGGCACGGGTTAGGGTTTCTTGCGAATTCGAAAGAGAAGGATAGAAAGAATTCGAAAGAAAAAGAGAAAGAGAAGAGGAAAGATGGAGATGTGGGGAAGGAAGTGAGGATCGTTTCGGGTCGAGACCATCTGTTGAGTAAGAGCAAACAGCATGATGAAGAGGTAGTAAAAGTGAATGCGGATGAAGTTGCGGAATTGGGTTCGAAAGAAGAGGAGAAGTGCTTGAGGAGATTGAATGAGACACAAAGGAAAATGGGTAGTGATAGCAGGCCGAGACGAGAAGAGCCAAGGGGTCATTCGACGTGGCTTGCGAGGAACATTCGGGTCAGGGTTATTAGCAAGGACTTGAAAGGTGGGAAATTGTATTCGAGAAAAGGGGAGGTGATGGATGTTGTTGGACCCTACACTTGCGATATATCCATGGATGGAAGTAGGGAGCTTGTGCAGGGGGTTTCTCAGGATTTTCTTGAGACAGCCCTGCCAAGGCGCGGCGGACCGGTTCTTGTGTTATGTGGGAAGCATAAGGGGGTTTATGGTAGTCTTGTGGAGAAGGATTCTGACAGAGAGACTGGTGTTGTCAAGGATTCTGATACCCATGCCTTGCTCAATGTGGGTCTTGAGCAGATTGCCGAGTTTACTGGTGATCCCAACGACCTTGGATATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009870 GO:0009987 GO:0023052 GO:0031347 GO:0031349 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045088 GO:0045089 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0080134 GO:0097159 GO:0098542 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

G-patch_2 KOW

Protein Analysis

425

Amino Acids

47.43

Weight (kDa)

6.46

Isoelectric Point (pI)

35.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
G-patch_2	PF12656	154 - 209	6.1e-17	G-patch domain
KN17_SH3	PF18131	304 - 351	4e-06	KN17 SH3-like domain
GPKOW_C	PF25088	310 - 417	6.8e-35	GPKOW C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006074)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33520
fragaria_vesca	FvH4_3g22160 FvH4_3g22160 FvH4_3g22160 FvH4_3g22160 FvH4_3g22160 FvH4_3g22160
malus_domestica	MD03G1208000.v1.1 MD11G1222200.v1.1
prunus_persica	Prupe.4G208800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0038051
rosa_laevigata	RLG00000033832
rosa_multiflora	Rmu_sc0002670.1_g000010 Rmu_sc0008278.1_g000003
rosa_roxburghii	Rroxscaffold_1G00042880
rosa_rugosa	Rorug05G0170200 Rorug05G0170300 Rorug05G0170400 Rorug05G0170500 Rorug05G0170600 Rorug05G0170600 Rorug05G0170700
rosa_samantha	Rh5AG258300 Rh5BG261700 Rh5CG293700 Rh5DG270700
rosa_wichuraiana	Rw5G024150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	537
AccB1I	GGYRCC	2 cut(s)	92, 537
AccB7I	CCANNNNNTGG	1 cut(s)	207
AccI	GTMKAC	1 cut(s)	593
AccII	CGCG	2 cut(s)	183, 1098
AciI	CCGC	9 cut(s)	69, 165, 181, 211, 261, 788, 800, 1098, 1101
AclWI	GGATC	4 cut(s)	190, 468, 725, 1247
AcoI	YGGCCR	2 cut(s)	262, 392
AcsI	RAATTY	3 cut(s)	140, 634, 658
AcyI	GRCGYC	3 cut(s)	114, 147, 315
AfaI	GTAC	4 cut(s)	329, 530, 539, 604
AfeI	AGCGCT	1 cut(s)	452
AfiI	CCNNNNNNNGG	7 cut(s)	207, 280, 500, 531, 548, 1092, 1097
AgeI	ACCGGT	1 cut(s)	1105
AgsI	TTSAA	4 cut(s)	85, 485, 844, 958
AjiI	CACGTC	1 cut(s)	912
AjuI	GAANNNNNNNTTGG	4 cut(s)	341, 373, 889, 921
AluBI	AGCT	3 cut(s)	241, 271, 1048
AluI	AGCT	3 cut(s)	241, 271, 1048
Alw21I	GWGCWC	1 cut(s)	273
Alw26I	GTCTC	6 cut(s)	281, 726, 842, 877, 1073, 1169
AlwI	GGATC	4 cut(s)	190, 468, 725, 1247
Ama87I	CYCGRG	1 cut(s)	541
Aor51HI	AGCGCT	1 cut(s)	452
AoxI	GGCC	4 cut(s)	212, 262, 392, 878
ApeKI	GCWGC	1 cut(s)	424
ApoI	RAATTY	3 cut(s)	140, 634, 658
ArsI	GACNNNNNNTTYG	2 cut(s)	117, 149
AsiGI	ACCGGT	1 cut(s)	1105
Asp700I	GAANNNNTTC	2 cut(s)	158, 658
Asp718I	GGTACC	1 cut(s)	537
AspLEI	GCGC	2 cut(s)	453, 1098
AspS9I	GGNCC	3 cut(s)	213, 1007, 1103
AsuC2I	CCSGG	4 cut(s)	344, 526, 542, 543
AsuHPI	GGTGA	5 cut(s)	241, 292, 398, 1003, 1262
AsuII	TTCGAA	3 cut(s)	638, 662, 812
AvaI	CYCGRG	1 cut(s)	541
AvaII	GGWCC	2 cut(s)	1007, 1103
BaeI	ACNNNNGTAYC	2 cut(s)	529, 562
BanI	GGYRCC	2 cut(s)	92, 537
BanII	GRGCYC	1 cut(s)	273
BauI	CACGAG	1 cut(s)	266
Bbv12I	GWGCWC	1 cut(s)	273
BbvI	GCAGC	1 cut(s)	411
BccI	CCATC	5 cut(s)	254, 686, 744, 988, 1028
BceAI	ACGGC	3 cut(s)	101, 106, 523
BciVI	GTATCC	1 cut(s)	409
BcnI	CCSGG	4 cut(s)	344, 526, 542, 543
BcoDI	GTCTC	6 cut(s)	281, 726, 842, 877, 1073, 1169
BfaI	CTAG	1 cut(s)	597
BfmI	CTRYAG	1 cut(s)	425
BfoI	RGCGCY	1 cut(s)	454
BfuI	GTATCC	1 cut(s)	409
BisI	GCNGC	4 cut(s)	212, 262, 425, 1099
BlsI	GCNGC	4 cut(s)	213, 263, 426, 1100
BmcAI	AGTACT	1 cut(s)	604
Bme1390I	CCNGG	4 cut(s)	344, 526, 542, 543
Bme18I	GGWCC	2 cut(s)	1007, 1103
BmeT110I	CYCGRG	1 cut(s)	541
BmgBI	CACGTC	1 cut(s)	912
BmgT120I	GGNCC	3 cut(s)	213, 1007, 1103
BmiI	GGNNCC	4 cut(s)	94, 215, 539, 1009
BmrFI	CCNGG	4 cut(s)	344, 526, 542, 543
BmsI	GCATC	1 cut(s)	480
BplI	GAGNNNNNCTC	2 cut(s)	278, 310
BpmI	CTGGAG	1 cut(s)	257
Bpu14I	TTCGAA	3 cut(s)	638, 662, 812
BpuEI	CTTGAG	3 cut(s)	853, 1097, 1247
BpuMI	CCSGG	4 cut(s)	344, 526, 542, 543
BsaHI	GRCGYC	3 cut(s)	114, 147, 315
BsaI	GGTCTC	1 cut(s)	726
BsaJI	CCNNGG	5 cut(s)	541, 896, 1029, 1091, 1264
BsaWI	WCCGGW	2 cut(s)	95, 1105
BsaXI	ACNNNNNCTCC	4 cut(s)	50, 80, 440, 470
Bsc4I	CCNNNNNNNGG	7 cut(s)	207, 280, 500, 531, 548, 1092, 1097
Bse118I	RCCGGY	3 cut(s)	390, 493, 1105
Bse1I	ACTGG	3 cut(s)	274, 1183, 1252
BseDI	CCNNGG	5 cut(s)	541, 896, 1029, 1091, 1264
BseGI	GGATG	4 cut(s)	32, 796, 1003, 1039
BseLI	CCNNNNNNNGG	7 cut(s)	207, 280, 500, 531, 548, 1092, 1097
BseMII	CTCAG	1 cut(s)	1079
BseNI	ACTGG	3 cut(s)	274, 1183, 1252
BseRI	GAGGAG	4 cut(s)	55, 491, 836, 850
BseX3I	CGGCCG	1 cut(s)	392
BseXI	GCAGC	1 cut(s)	411
BsgI	GTGCAG	1 cut(s)	1073
Bsh1236I	CGCG	2 cut(s)	183, 1098
Bsh1285I	CGRYCG	1 cut(s)	395
BshFI	GGCC	4 cut(s)	214, 264, 394, 880
BshNI	GGYRCC	2 cut(s)	92, 537
BshTI	ACCGGT	1 cut(s)	1105
BsiEI	CGRYCG	1 cut(s)	395
BsiHKAI	GWGCWC	1 cut(s)	273
BsiHKCI	CYCGRG	1 cut(s)	541
BsiSI	CCGG	8 cut(s)	96, 258, 343, 391, 494, 525, 542, 1106
BsiWI	CGTACG	1 cut(s)	327
BslI	CCNNNNNNNGG	7 cut(s)	207, 280, 500, 531, 548, 1092, 1097
BsmAI	GTCTC	6 cut(s)	281, 726, 842, 877, 1073, 1169
BsmBI	CGTCTC	2 cut(s)	281, 877
BsmI	GAATGC	2 cut(s)	568, 790
BsnI	GGCC	4 cut(s)	214, 264, 394, 880
Bso31I	GGTCTC	1 cut(s)	726
BsoBI	CYCGRG	1 cut(s)	541
Bsp119I	TTCGAA	3 cut(s)	638, 662, 812
Bsp1286I	GDGCHC	1 cut(s)	273
Bsp143I	GATC	5 cut(s)	186, 195, 460, 717, 1252
Bsp19I	CCATGG	1 cut(s)	1029
BspACI	CCGC	9 cut(s)	69, 165, 181, 211, 261, 788, 800, 1098, 1101
BspANI	GGCC	4 cut(s)	214, 264, 394, 880
BspCNI	CTCAG	1 cut(s)	1078
BspFNI	CGCG	2 cut(s)	183, 1098
BspLI	GGNNCC	4 cut(s)	94, 215, 539, 1009
BspMAI	CTGCAG	1 cut(s)	429
BspPI	GGATC	4 cut(s)	190, 468, 725, 1247
BspQI	GCTCTTC	1 cut(s)	885
BspT104I	TTCGAA	3 cut(s)	638, 662, 812
BspT107I	GGYRCC	2 cut(s)	92, 537
BspTNI	GGTCTC	1 cut(s)	726
BsrFI	RCCGGY	3 cut(s)	390, 493, 1105
BsrI	ACTGG	3 cut(s)	274, 1183, 1252
BssAI	RCCGGY	3 cut(s)	390, 493, 1105
BssECI	CCNNGG	5 cut(s)	541, 896, 1029, 1091, 1264
BssMI	GATC	5 cut(s)	186, 195, 460, 717, 1252
BssNAI	GTATAC	1 cut(s)	594
BssNI	GRCGYC	3 cut(s)	114, 147, 315
BssSI	CACGAG	1 cut(s)	266
BssT1I	CCWWGG	4 cut(s)	896, 1029, 1091, 1264
Bst1107I	GTATAC	1 cut(s)	594
Bst2BI	CACGAG	1 cut(s)	266
Bst4CI	ACNGT	1 cut(s)	332
Bst6I	CTCTTC	5 cut(s)	480, 677, 762, 813, 885
BstACI	GRCGYC	3 cut(s)	114, 147, 315
BstBI	TTCGAA	3 cut(s)	638, 662, 812
BstC8I	GCNNGC	3 cut(s)	181, 878, 918
BstDEI	CTNAG	1 cut(s)	1065
BstDSI	CCRYGG	1 cut(s)	1029
BstEII	GGTNACC	1 cut(s)	386
BstF5I	GGATG	4 cut(s)	32, 796, 1003, 1039
BstFNI	CGCG	2 cut(s)	183, 1098
BstH2I	RGCGCY	1 cut(s)	454
BstHHI	GCGC	2 cut(s)	453, 1098
BstKTI	GATC	5 cut(s)	189, 198, 463, 720, 1255
BstMAI	GTCTC	6 cut(s)	281, 726, 842, 877, 1073, 1169
BstMBI	GATC	5 cut(s)	186, 195, 460, 717, 1252
BstMCI	CGRYCG	1 cut(s)	395
BstPI	GGTNACC	1 cut(s)	386
BstSCI	CCNGG	4 cut(s)	342, 524, 540, 541
BstSFI	CTRYAG	1 cut(s)	425
BstUI	CGCG	2 cut(s)	183, 1098
BstV1I	GCAGC	1 cut(s)	411
BstZ17I	GTATAC	1 cut(s)	594
BstZI	CGGCCG	1 cut(s)	392
BsuI	GTATCC	1 cut(s)	409
BsuRI	GGCC	4 cut(s)	214, 264, 394, 880
BtgI	CCRYGG	1 cut(s)	1029
BtrI	CACGTC	1 cut(s)	912
BtsCI	GGATG	4 cut(s)	32, 796, 1003, 1039
Cac8I	GCNNGC	3 cut(s)	181, 878, 918
CfoI	GCGC	2 cut(s)	453, 1098
Cfr10I	RCCGGY	3 cut(s)	390, 493, 1105
Cfr13I	GGNCC	3 cut(s)	213, 1007, 1103
Cfr9I	CCCGGG	1 cut(s)	541
CpoI	CGGWCCG	1 cut(s)	1103
CseI	GACGC	4 cut(s)	122, 155, 298, 323
Csp6I	GTAC	4 cut(s)	328, 529, 538, 603
CspAI	ACCGGT	1 cut(s)	1105
CspI	CGGWCCG	1 cut(s)	1103
CviAII	CATG	3 cut(s)	761, 1030, 1205
CviQI	GTAC	4 cut(s)	328, 529, 538, 603
DdeI	CTNAG	1 cut(s)	1065
DpnI	GATC	5 cut(s)	188, 197, 462, 719, 1254
DpnII	GATC	5 cut(s)	186, 195, 460, 717, 1252
DraI	TTTAAA	1 cut(s)	436
EaeI	YGGCCR	2 cut(s)	262, 392
EagI	CGGCCG	1 cut(s)	392
Eam1104I	CTCTTC	5 cut(s)	480, 677, 762, 813, 885
EarI	CTCTTC	5 cut(s)	480, 677, 762, 813, 885
EciI	GGCGGA	3 cut(s)	58, 154, 1116
Ecl136II	GAGCTC	1 cut(s)	271
EclXI	CGGCCG	1 cut(s)	392
Eco130I	CCWWGG	4 cut(s)	896, 1029, 1091, 1264
Eco24I	GRGCYC	1 cut(s)	273
Eco31I	GGTCTC	1 cut(s)	726
Eco47I	GGWCC	2 cut(s)	1007, 1103
Eco47III	AGCGCT	1 cut(s)	452
Eco52I	CGGCCG	1 cut(s)	392
Eco53kI	GAGCTC	1 cut(s)	271
Eco88I	CYCGRG	1 cut(s)	541
Eco91I	GGTNACC	1 cut(s)	386
EcoICRI	GAGCTC	1 cut(s)	271
EcoO65I	GGTNACC	1 cut(s)	386
EcoRI	GAATTC	3 cut(s)	140, 634, 658
EcoT14I	CCWWGG	4 cut(s)	896, 1029, 1091, 1264
EcoT38I	GRGCYC	1 cut(s)	273
ErhI	CCWWGG	4 cut(s)	896, 1029, 1091, 1264
Esp3I	CGTCTC	2 cut(s)	281, 877
FaeI	CATG	3 cut(s)	764, 1033, 1208
FaiI	YATR	9 cut(s)	465, 594, 762, 1025, 1031, 1120, 1131, 1143, 1206
FatI	CATG	3 cut(s)	760, 1029, 1204
FauI	CCCGC	1 cut(s)	188
FblI	GTMKAC	1 cut(s)	593
Fnu4HI	GCNGC	4 cut(s)	212, 262, 425, 1099
FokI	GGATG	4 cut(s)	19, 803, 1010, 1046
FriOI	GRGCYC	1 cut(s)	273
Fsp4HI	GCNGC	4 cut(s)	212, 262, 425, 1099
FspBI	CTAG	1 cut(s)	597
GlaI	GCGC	2 cut(s)	452, 1097
GluI	GCNGC	4 cut(s)	212, 262, 425, 1099
GsuI	CTGGAG	1 cut(s)	257
HaeII	RGCGCY	1 cut(s)	454
HaeIII	GGCC	4 cut(s)	214, 264, 394, 880
HapII	CCGG	8 cut(s)	96, 258, 343, 391, 494, 525, 542, 1106
HgaI	GACGC	4 cut(s)	122, 155, 298, 323
HhaI	GCGC	2 cut(s)	453, 1098
Hin1I	GRCGYC	3 cut(s)	114, 147, 315
Hin1II	CATG	3 cut(s)	764, 1033, 1208
Hin6I	GCGC	2 cut(s)	451, 1096
HinP1I	GCGC	2 cut(s)	451, 1096
HinfI	GANTC	4 cut(s)	254, 364, 1163, 1193
HpaII	CCGG	8 cut(s)	96, 258, 343, 391, 494, 525, 542, 1106
HphI	GGTGA	5 cut(s)	241, 292, 398, 1003, 1262
Hpy166II	GTNNAC	2 cut(s)	594, 1245
Hpy188I	TCNGA	4 cut(s)	355, 460, 1168, 1198
Hpy188III	TCNNGA	7 cut(s)	286, 368, 731, 978, 1067, 1076, 1226
Hpy8I	GTNNAC	2 cut(s)	594, 1245
Hpy99I	CGWCG	5 cut(s)	92, 149, 308, 317, 913
HpyAV	CCTTC	7 cut(s)	22, 91, 169, 562, 640, 700, 1153
HpyCH4III	ACNGT	1 cut(s)	332
HpyCH4IV	ACGT	1 cut(s)	911
HpyCH4V	TGCA	2 cut(s)	427, 1054
HpyF3I	CTNAG	1 cut(s)	1065
HpySE526I	ACGT	1 cut(s)	911
Hsp92I	GRCGYC	3 cut(s)	114, 147, 315
Hsp92II	CATG	3 cut(s)	764, 1033, 1208
HspAI	GCGC	2 cut(s)	451, 1096
KpnI	GGTACC	1 cut(s)	541
Kzo9I	GATC	5 cut(s)	186, 195, 460, 717, 1252
LguI	GCTCTTC	1 cut(s)	885
LmnI	GCTCC	3 cut(s)	276, 448, 1045
Lsp1109I	GCAGC	1 cut(s)	411
LweI	GCATC	1 cut(s)	480
MaeI	CTAG	1 cut(s)	597
MaeII	ACGT	1 cut(s)	911
MaeIII	GTNAC	3 cut(s)	298, 386, 503
MalI	GATC	5 cut(s)	188, 197, 462, 719, 1254
MboI	GATC	5 cut(s)	186, 195, 460, 717, 1252
MhlI	GDGCHC	1 cut(s)	273
MluCI	AATT	7 cut(s)	140, 356, 552, 634, 658, 803, 968
MlyI	GAGTC	1 cut(s)	263
MmeI	TCCRAC	3 cut(s)	438, 453, 985
MroXI	GAANNNNTTC	2 cut(s)	158, 658
MseI	TTAA	2 cut(s)	336, 435
MslI	CAYNNNNRTG	1 cut(s)	108
MspI	CCGG	8 cut(s)	96, 258, 343, 391, 494, 525, 542, 1106
MspR9I	CCNGG	4 cut(s)	344, 526, 542, 543
Mva1269I	GAATGC	2 cut(s)	568, 790
MvnI	CGCG	2 cut(s)	183, 1098
NciI	CCSGG	4 cut(s)	344, 526, 542, 543
NcoI	CCATGG	1 cut(s)	1029
NdeII	GATC	5 cut(s)	186, 195, 460, 717, 1252
NlaIII	CATG	3 cut(s)	764, 1033, 1208
NlaIV	GGNNCC	4 cut(s)	94, 215, 539, 1009
NmeAIII	GCCGAG	3 cut(s)	420, 906, 1264
NmuCI	GTSAC	2 cut(s)	298, 386
NspV	TTCGAA	3 cut(s)	638, 662, 812
PciSI	GCTCTTC	1 cut(s)	885
PcsI	WCGNNNNNNNCGW	1 cut(s)	917
PctI	GAATGC	2 cut(s)	568, 790
PdmI	GAANNNNTTC	2 cut(s)	158, 658
PfeI	GAWTC	3 cut(s)	364, 1163, 1193
Pfl23II	CGTACG	1 cut(s)	327
PflMI	CCANNNNNTGG	1 cut(s)	207
PfoI	TCCNGGA	1 cut(s)	342
PinAI	ACCGGT	1 cut(s)	1105
PkrI	GCNGC	4 cut(s)	213, 263, 426, 1100
PleI	GAGTC	1 cut(s)	262
PpsI	GAGTC	1 cut(s)	262
Psp124BI	GAGCTC	1 cut(s)	273
PspEI	GGTNACC	1 cut(s)	386
PspLI	CGTACG	1 cut(s)	327
PspN4I	GGNNCC	4 cut(s)	94, 215, 539, 1009
PspPI	GGNCC	3 cut(s)	213, 1007, 1103
PstI	CTGCAG	1 cut(s)	429
RsaI	GTAC	4 cut(s)	329, 530, 539, 604
RsaNI	GTAC	4 cut(s)	328, 529, 538, 603
RseI	CAYNNNNRTG	1 cut(s)	108
Rsr2I	CGGWCCG	1 cut(s)	1103
RsrII	CGGWCCG	1 cut(s)	1103
SacI	GAGCTC	1 cut(s)	273
SapI	GCTCTTC	1 cut(s)	885
SaqAI	TTAA	2 cut(s)	336, 435
SatI	GCNGC	4 cut(s)	212, 262, 425, 1099
Sau3AI	GATC	5 cut(s)	186, 195, 460, 717, 1252
Sau96I	GGNCC	3 cut(s)	213, 1007, 1103
ScaI	AGTACT	1 cut(s)	604
SchI	GAGTC	1 cut(s)	263
ScrFI	CCNGG	4 cut(s)	344, 526, 542, 543
SduI	GDGCHC	1 cut(s)	273
SfaNI	GCATC	1 cut(s)	480
SfcI	CTRYAG	1 cut(s)	425
SfuI	TTCGAA	3 cut(s)	638, 662, 812
SinI	GGWCC	2 cut(s)	1007, 1103
SmaI	CCCGGG	1 cut(s)	543
SmiMI	CAYNNNNRTG	1 cut(s)	108
SmlI	CTYRAG	3 cut(s)	832, 1076, 1226
SmoI	CTYRAG	3 cut(s)	832, 1076, 1226
Sse9I	AATT	7 cut(s)	140, 356, 552, 634, 658, 803, 968
SsiI	CCGC	9 cut(s)	69, 165, 181, 211, 261, 788, 800, 1098, 1101
SspMI	CTAG	1 cut(s)	597
SstI	GAGCTC	1 cut(s)	273
StyD4I	CCNGG	4 cut(s)	342, 524, 540, 541
StyI	CCWWGG	4 cut(s)	896, 1029, 1091, 1264
TaaI	ACNGT	1 cut(s)	332
TaiI	ACGT	1 cut(s)	914
TasI	AATT	7 cut(s)	140, 356, 552, 634, 658, 803, 968
TatI	WGTACW	1 cut(s)	602
TauI	GCSGC	3 cut(s)	214, 264, 1101
TfiI	GAWTC	3 cut(s)	364, 1163, 1193
Tru1I	TTAA	2 cut(s)	336, 435
Tru9I	TTAA	2 cut(s)	336, 435
TseFI	GTSAC	2 cut(s)	298, 386
TseI	GCWGC	1 cut(s)	424
Tsp45I	GTSAC	2 cut(s)	298, 386
TspDTI	ATGAA	4 cut(s)	17, 242, 780, 807
TspGWI	ACGGA	1 cut(s)	286
TspMI	CCCGGG	1 cut(s)	541
Van91I	CCANNNNNTGG	1 cut(s)	207
VpaK11BI	GGWCC	2 cut(s)	1007, 1103
XapI	RAATTY	3 cut(s)	140, 634, 658
XmaI	CCCGGG	1 cut(s)	541
XmiI	GTMKAC	1 cut(s)	593
XmnI	GAANNNNTTC	2 cut(s)	158, 658
XspI	CTAG	1 cut(s)	597
ZrmI	AGTACT	1 cut(s)	604

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.