MD03G1211500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004475
Physical Location & Seq
Forward (+)
29051192 .. 29051848
657 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1211500.v1.1.491

Sequence Viewer

Length: 657 bp
ATGGCTTCCATCTTCCCACAATTCCTCTCAACTCATAATCCTCCTCACCCACCACCAAACCACCTCCCAACCCAAACCACCGCAGCTCCCTCCCACCTTTCCCTCCATAGACCACCCAGGAAACACTTGTCCACCACCACATGCGCCACTCCAAACCCTACTCAACCTGACTTCCCAAGTCCAGACTCCGAAACCACCACCAGCGCCGAGACATTCCCCATCGAAAAGCGAAGAAAATCCGACATCACCCGAGACAGGAGCAGGTCAGAAACCGGCCTCGCAAAGCCGGAACCCCCCAACTTTGAGATTGGTTGGAAGAGAACCAAAGAGATAAAGCTAGAGAAGCCGATAGGGTACGTAATAGCTGACTTCTTGGAGAAGTTAGAGGACCTGATGGGAAAAGAATTTGGCTCCACTGAACTGTTGCTAAAAGTGGCGGAGATCGTGGCTGAGAGAGCGAGAGAGGAAGCGCAAGTGTTGAGAGACGAAGGCAAAGTGGAGGACAGAATGGTGACCGAGCTGTTTAGGGTTTTGAGGTTGATAGAGATGGACATGGTTATGGTAAAAGCTGCTGTCAAGGAAGAGACTTTGAATGAGAGGCTTGAGCAGGCTAAGGCAAGGTGCAGGCAAGCTATACTTGTTGCTTTGTCGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

219

Amino Acids

24.7

Weight (kDa)

6.62

Isoelectric Point (pI)

49.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 252
AciI CCGC 2 cut(s) 81, 437
AcsI RAATTY 1 cut(s) 404
AfaI GTAC 1 cut(s) 356
AfiI CCNNNNNNNGG 1 cut(s) 255
AgsI TTSAA 1 cut(s) 592
AjnI CCWGG 1 cut(s) 116
AluBI AGCT 6 cut(s) 86, 337, 365, 520, 569, 632
AluI AGCT 6 cut(s) 86, 337, 365, 520, 569, 632
Alw26I GTCTC 4 cut(s) 203, 246, 477, 578
Ama87I CYCGRG 1 cut(s) 249
AoxI GGCC 1 cut(s) 274
ApeKI GCWGC 2 cut(s) 83, 569
ApoI RAATTY 1 cut(s) 404
AspLEI GCGC 3 cut(s) 146, 206, 472
AspS9I GGNCC 1 cut(s) 388
AsuHPI GGTGA 3 cut(s) 38, 238, 523
AvaI CYCGRG 1 cut(s) 249
AvaII GGWCC 1 cut(s) 388
BbvI GCAGC 2 cut(s) 95, 556
BccI CCATC 4 cut(s) 17, 227, 388, 541
BciT130I CCWGG 1 cut(s) 118
BcoDI GTCTC 4 cut(s) 203, 246, 477, 578
BfaI CTAG 1 cut(s) 338
BfoI RGCGCY 1 cut(s) 207
BfuAI ACCTGC 1 cut(s) 252
BisI GCNGC 2 cut(s) 84, 570
BlsI GCNGC 2 cut(s) 85, 571
Bme1390I CCNGG 1 cut(s) 118
Bme18I GGWCC 1 cut(s) 388
BmeT110I CYCGRG 1 cut(s) 249
BmgT120I GGNCC 1 cut(s) 388
BmiI GGNNCC 2 cut(s) 291, 412
BmrFI CCNGG 1 cut(s) 118
Bpu10I CCTNAGC 1 cut(s) 612
BpuEI CTTGAG 1 cut(s) 623
BsaAI YACGTR 1 cut(s) 358
BsaJI CCNNGG 1 cut(s) 116
BsaXI ACNNNNNCTCC 2 cut(s) 70, 100
Bsc4I CCNNNNNNNGG 1 cut(s) 255
Bse118I RCCGGY 1 cut(s) 272
BseBI CCWGG 1 cut(s) 118
BseDI CCNNGG 1 cut(s) 116
BseLI CCNNNNNNNGG 1 cut(s) 255
BseMII CTCAG 1 cut(s) 441
BseRI GAGGAG 1 cut(s) 33
BseXI GCAGC 2 cut(s) 95, 556
BsgI GTGCAG 1 cut(s) 643
BshFI GGCC 1 cut(s) 276
BsiHKCI CYCGRG 1 cut(s) 249
BsiSI CCGG 2 cut(s) 273, 287
BslI CCNNNNNNNGG 1 cut(s) 255
BsmAI GTCTC 4 cut(s) 203, 246, 477, 578
BsmBI CGTCTC 1 cut(s) 477
BsnI GGCC 1 cut(s) 276
BsoBI CYCGRG 1 cut(s) 249
Bsp143I GATC 1 cut(s) 441
BspACI CCGC 2 cut(s) 81, 437
BspANI GGCC 1 cut(s) 276
BspCNI CTCAG 1 cut(s) 442
BspLI GGNNCC 2 cut(s) 291, 412
BspMI ACCTGC 1 cut(s) 252
BsrFI RCCGGY 1 cut(s) 272
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 1 cut(s) 116
BssMI GATC 1 cut(s) 441
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 1 cut(s) 423
Bst6I CTCTTC 2 cut(s) 311, 576
BstBAI YACGTR 1 cut(s) 358
BstC8I GCNNGC 3 cut(s) 609, 626, 630
BstDEI CTNAG 2 cut(s) 450, 612
BstEII GGTNACC 1 cut(s) 511
BstH2I RGCGCY 1 cut(s) 207
BstHHI GCGC 3 cut(s) 146, 206, 472
BstKTI GATC 1 cut(s) 444
BstMAI GTCTC 4 cut(s) 203, 246, 477, 578
BstMBI GATC 1 cut(s) 441
BstMWI GCNNNNNNNGC 2 cut(s) 343, 455
BstNI CCWGG 1 cut(s) 118
BstNSI RCATGY 1 cut(s) 144
BstPI GGTNACC 1 cut(s) 511
BstSCI CCNGG 1 cut(s) 116
BstSNI TACGTA 1 cut(s) 358
BstV1I GCAGC 2 cut(s) 95, 556
BsuRI GGCC 1 cut(s) 276
BtsIMutI CAGTG 1 cut(s) 414
BveI ACCTGC 1 cut(s) 252
Cac8I GCNNGC 3 cut(s) 609, 626, 630
CfoI GCGC 3 cut(s) 146, 206, 472
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 1 cut(s) 388
Csp6I GTAC 1 cut(s) 355
CviAII CATG 2 cut(s) 141, 553
CviQI GTAC 1 cut(s) 355
DdeI CTNAG 2 cut(s) 450, 612
DpnI GATC 1 cut(s) 443
DpnII GATC 1 cut(s) 441
Eam1104I CTCTTC 2 cut(s) 311, 576
EarI CTCTTC 2 cut(s) 311, 576
EciI GGCGGA 1 cut(s) 452
Eco105I TACGTA 1 cut(s) 358
Eco47I GGWCC 1 cut(s) 388
Eco88I CYCGRG 1 cut(s) 249
Eco91I GGTNACC 1 cut(s) 511
EcoO109I RGGNCCY 1 cut(s) 388
EcoO65I GGTNACC 1 cut(s) 511
EcoRII CCWGG 1 cut(s) 116
Esp3I CGTCTC 1 cut(s) 477
FaeI CATG 2 cut(s) 144, 556
FaiI YATR 6 cut(s) 36, 108, 142, 554, 560, 635
FalI AAGNNNNNCTT 2 cut(s) 621, 653
FatI CATG 2 cut(s) 140, 552
Fnu4HI GCNGC 2 cut(s) 84, 570
Fsp4HI GCNGC 2 cut(s) 84, 570
FspBI CTAG 1 cut(s) 338
GlaI GCGC 3 cut(s) 145, 205, 471
GluI GCNGC 2 cut(s) 84, 570
HaeII RGCGCY 1 cut(s) 207
HaeIII GGCC 1 cut(s) 276
HapII CCGG 2 cut(s) 273, 287
HhaI GCGC 3 cut(s) 146, 206, 472
Hin1II CATG 2 cut(s) 144, 556
Hin6I GCGC 3 cut(s) 144, 204, 470
HinP1I GCGC 3 cut(s) 144, 204, 470
HinfI GANTC 1 cut(s) 185
HpaII CCGG 2 cut(s) 273, 287
HphI GGTGA 3 cut(s) 38, 238, 523
Hpy166II GTNNAC 1 cut(s) 132
Hpy188I TCNGA 4 cut(s) 190, 241, 268, 656
Hpy188III TCNNGA 1 cut(s) 182
Hpy8I GTNNAC 1 cut(s) 132
HpyAV CCTTC 1 cut(s) 482
HpyCH4III ACNGT 1 cut(s) 423
HpyCH4IV ACGT 1 cut(s) 357
HpyCH4V TGCA 1 cut(s) 624
HpyF10VI GCNNNNNNNGC 2 cut(s) 343, 455
HpyF3I CTNAG 2 cut(s) 450, 612
HpySE526I ACGT 1 cut(s) 357
Hsp92II CATG 2 cut(s) 144, 556
HspAI GCGC 3 cut(s) 144, 204, 470
Kzo9I GATC 1 cut(s) 441
LmnI GCTCC 3 cut(s) 91, 258, 416
Lsp1109I GCAGC 2 cut(s) 95, 556
MaeI CTAG 1 cut(s) 338
MaeII ACGT 1 cut(s) 357
MaeIII GTNAC 1 cut(s) 511
MalI GATC 1 cut(s) 443
MboI GATC 1 cut(s) 441
MboII GAAGA 4 cut(s) 4, 243, 328, 593
MluCI AATT 2 cut(s) 20, 404
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 2 cut(s) 264, 293
MslI CAYNNNNRTG 1 cut(s) 557
MspI CCGG 2 cut(s) 273, 287
MspR9I CCNGG 1 cut(s) 118
MvaI CCWGG 1 cut(s) 118
MwoI GCNNNNNNNGC 2 cut(s) 343, 455
NdeII GATC 1 cut(s) 441
NlaIII CATG 2 cut(s) 144, 556
NlaIV GGNNCC 2 cut(s) 291, 412
NmeAIII GCCGAG 1 cut(s) 232
NmuCI GTSAC 1 cut(s) 511
NspI RCATGY 1 cut(s) 144
PkrI GCNGC 2 cut(s) 85, 571
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
Ppu21I YACGTR 1 cut(s) 358
PpuMI RGGWCCY 1 cut(s) 388
Psp5II RGGWCCY 1 cut(s) 388
Psp6I CCWGG 1 cut(s) 116
PspEI GGTNACC 1 cut(s) 511
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 2 cut(s) 291, 412
PspPI GGNCC 1 cut(s) 388
PspPPI RGGWCCY 1 cut(s) 388
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
RseI CAYNNNNRTG 1 cut(s) 557
SatI GCNGC 2 cut(s) 84, 570
Sau3AI GATC 1 cut(s) 441
Sau96I GGNCC 1 cut(s) 388
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 1 cut(s) 118
SinI GGWCC 1 cut(s) 388
SmiMI CAYNNNNRTG 1 cut(s) 557
SmlI CTYRAG 1 cut(s) 602
SmoI CTYRAG 1 cut(s) 602
SnaBI TACGTA 1 cut(s) 358
Sse9I AATT 2 cut(s) 20, 404
SsiI CCGC 2 cut(s) 81, 437
SspMI CTAG 1 cut(s) 338
StyD4I CCNGG 1 cut(s) 116
TaaI ACNGT 1 cut(s) 423
TaiI ACGT 1 cut(s) 360
TaqI TCGA 1 cut(s) 222
TaqII GACCGA 1 cut(s) 530
TasI AATT 2 cut(s) 20, 404
TscAI CASTG 1 cut(s) 421
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 2 cut(s) 83, 569
Tsp45I GTSAC 1 cut(s) 511
TspRI CASTG 1 cut(s) 421
VpaK11BI GGWCC 1 cut(s) 388
XapI RAATTY 1 cut(s) 404
XceI RCATGY 1 cut(s) 144
XspI CTAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.