Rorug05G0165500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004475
Physical Location & Seq
Forward (+)
15326558 .. 15328345
1788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0165500.1

Sequence Viewer

Length: 1701 bp
ATGGACCTCTGCAATACAAGTCCAGCTCCATCTATTGATGCTGAAGACCGGGAGTTGGAGGCTTATTATGAGTCCTTTTTGGGGGGTAAGATAAATCTTTCTTTGCTTAGCTTGAAATATATGAGTTACTTAGACTTGAGCTCGAATGACTTTCAAGGGCTTCAAATTCCTAAGTTCTTTGGTCAGCTTCAAAGTCTGAACGGTAACTACTTATTGGAAATTTCTTCCATAAATTTGAATTGGCTTTCTCATCTCTCCTCTCTAAAATACCTCAATCTGAATGAAGTGGACCTTAGCAATGCAGGAGCCAGTTGGCTACATGTTGTTAACATGCTTCCTTCCATGCTAGAGTTACACTTGTCTAGATGCAACATTGAAAGCCTTCCACCTTCACTTCACACCATGAAACTCACATCACTTTTGATCCTTGATATGTCATTCAATGATTTTAAGTCTCCATTTCCAAAGTGGGTCGTTAATCTAACTAGCCTCAGAGAACTTCACTTGGCCAAGAATTCTTTCAGCGGTCCCTTTCCCAATGATTTCATTAACCTGAAATCATTGGAAGAACTTGATTTATCTCTTAATGGCCTTCACGGTCCAATTCCCAAATTCTTTGGAAATCTCTGCAACCTAAAGACCCTAATTCTTGCTGGAAACGCATTTGATCAGGGTATTCCAGAGTTTTTGGATGGTTTATCAGGTTGTGCAAATAATAAGTTGGAGTCACTAGACTTGTCTAGTAATAAGCTGGTAGGCGAATTGCCTGCCTCCTTAGGGATGCTCCCCCATTTGCAGTACCTTATCCTCTTCGACAATCATTTGAATGGATCCATCCCGGAAAGTTTGGGACAACTCTCTGAGCTAGTTCATCTAGACCTGTCGGGCAACTCATTGGTAGGCTTTTTAACTGAAGCCCATTTGATAGATCTCGCGAAGTTAACATACATTGCGTTGGGCAATATCAGTGTTCCCAACCCGCTACCTTCCATCATTTTCAATGTGGGTTATGAGTGGGTTCCTCCTTTCAAGCTTGACACCATTGACATCAGGAACTGTCAAGTAGGTCTTGCCTTTTCACTATGGCTTCTATCTCAAACTGAACTAGTATATGTCGCTCTAAGCAATACAAGCATCTCGGATTCCATACCAGACGAATGGTTCATGAAAATATCTTCCCAACTCCAATTTTTGGATTTATCTTTCAACCAAATTCGTGGAAAGCTTCCAGTCCAACTGATAGTTCCAAAATTAGTGACTATAGATTTGAGACATAATCAATTCCACGGCTCACTCCCGCTTTGGTTGTGTAAGGAGGCTTCCTACCTAAATTTTGCTTTCAATTCATTTTCCGGGCCAATTCCATTTACTTTTGACCAACATTTTCCAAAATTGCTACAATTGCATCTCTATGAAAATTATTTGAATGGCACGATTCCATTCTCTGTTTGCAATATGCAGAAGTTGTTAATCTTTTCTCTAAGAAGCAACAACCTTTCTGGAGAATTCCCTCAAGCATGGAGTATGTGGGGTGATATATACATTGTAGATGTTGGAGACAATCATCTCTCAGGAAATATTCCCAGTTCAATGGGTATCCCAGGTTCACTTTTTATGTTGTCGATGAAGAACAACAAATTCGATAGTGAAATCCCGATGGCCTTGGGAAATTGCACCAACCTGAGGGGTCAGGAGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

566

Amino Acids

63.22

Weight (kDa)

5.05

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 72 - 297 5.9e-15 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1192
AccII CGCG 1 cut(s) 935
AciI CCGC 3 cut(s) 525, 980, 1298
AclWI GGATC 3 cut(s) 418, 825, 838
AcoI YGGCCR 1 cut(s) 507
AcsI RAATTY 9 cut(s) 165, 219, 232, 514, 611, 1212, 1330, 1505, 1637
AcuI CTGAAG 2 cut(s) 63, 933
AfaI GTAC 1 cut(s) 800
AfiI CCNNNNNNNGG 5 cut(s) 55, 81, 777, 1192, 1683
AflIII ACRYGT 1 cut(s) 319
AhlI ACTAGT 1 cut(s) 1105
AjnI CCWGG 1 cut(s) 1600
AjuI GAANNNNNNNTTGG 4 cut(s) 503, 535, 1227, 1259
AluBI AGCT 8 cut(s) 26, 111, 141, 187, 751, 865, 1033, 1225
AluI AGCT 8 cut(s) 26, 111, 141, 187, 751, 865, 1033, 1225
Alw21I GWGCWC 1 cut(s) 143
Alw26I GTCTC 3 cut(s) 459, 1264, 1551
AlwI GGATC 3 cut(s) 418, 825, 838
AlwNI CAGNNNCTG 1 cut(s) 1056
AoxI GGCC 4 cut(s) 507, 589, 1355, 1659
ApoI RAATTY 9 cut(s) 165, 219, 232, 514, 611, 1212, 1330, 1505, 1637
ArsI GACNNNNNNTTYG 2 cut(s) 1249, 1281
Asp700I GAANNNNTTC 2 cut(s) 381, 518
AspS9I GGNCC 5 cut(s) 4, 289, 527, 599, 1355
AsuC2I CCSGG 3 cut(s) 50, 839, 1354
AsuHPI GGTGA 1 cut(s) 1544
AvaII GGWCC 4 cut(s) 4, 289, 527, 599
AxyI CCTNAGG 2 cut(s) 775, 1682
BalI TGGCCA 1 cut(s) 509
BamHI GGATCC 1 cut(s) 830
BanII GRGCYC 1 cut(s) 143
BarI GAAGNNNNNNTAC 4 cut(s) 929, 961, 1303, 1335
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 1 cut(s) 143
BccI CCATC 5 cut(s) 37, 686, 842, 998, 1651
BceAI ACGGC 1 cut(s) 1303
BcgI CGANNNNNNTGC 2 cut(s) 749, 783
BciT130I CCWGG 1 cut(s) 1602
BciVI GTATCC 1 cut(s) 1607
BclI TGATCA 1 cut(s) 667
BcnI CCSGG 3 cut(s) 50, 839, 1354
BcoDI GTCTC 3 cut(s) 459, 1264, 1551
BcuI ACTAGT 1 cut(s) 1105
BfaI CTAG 8 cut(s) 347, 363, 486, 731, 741, 866, 875, 1106
BfmI CTRYAG 1 cut(s) 1260
BfuI GTATCC 1 cut(s) 1607
BglII AGATCT 1 cut(s) 928
BlpI GCTNAGC 1 cut(s) 107
Bme1390I CCNGG 4 cut(s) 50, 839, 1354, 1602
Bme18I GGWCC 4 cut(s) 4, 289, 527, 599
BmgT120I GGNCC 5 cut(s) 4, 289, 527, 599, 1355
BmiI GGNNCC 4 cut(s) 307, 529, 832, 1020
BmrFI CCNGG 4 cut(s) 50, 839, 1354, 1602
BmrI ACTGGG 1 cut(s) 1578
BmsI GCATC 5 cut(s) 28, 356, 771, 1143, 1414
BmuI ACTGGG 1 cut(s) 1578
BpiI GAAGAC 1 cut(s) 51
BpmI CTGGAG 1 cut(s) 1521
Bpu10I CCTNAGC 1 cut(s) 293
Bpu1102I GCTNAGC 1 cut(s) 107
BpuEI CTTGAG 2 cut(s) 157, 1497
BpuMI CCSGG 3 cut(s) 50, 839, 1354
BsaJI CCNNGG 3 cut(s) 1285, 1600, 1662
Bsc4I CCNNNNNNNGG 5 cut(s) 55, 81, 777, 1192, 1683
Bse1I ACTGG 3 cut(s) 309, 1229, 1584
Bse21I CCTNAGG 2 cut(s) 775, 1682
Bse3DI GCAATG 2 cut(s) 304, 948
BseBI CCWGG 1 cut(s) 1602
BseDI CCNNGG 3 cut(s) 1285, 1600, 1662
BseGI GGATG 3 cut(s) 697, 786, 834
BseLI CCNNNNNNNGG 5 cut(s) 55, 81, 777, 1192, 1683
BseMI GCAATG 2 cut(s) 304, 948
BseMII CTCAG 4 cut(s) 505, 852, 1584, 1673
BseNI ACTGG 3 cut(s) 309, 1229, 1584
BseRI GAGGAG 1 cut(s) 247
Bsh1236I CGCG 1 cut(s) 935
BshFI GGCC 4 cut(s) 509, 591, 1357, 1661
BsiHKAI GWGCWC 1 cut(s) 143
BsiSI CCGG 3 cut(s) 49, 839, 1353
BslFI GGGAC 2 cut(s) 513, 864
BslI CCNNNNNNNGG 5 cut(s) 55, 81, 777, 1192, 1683
BsmAI GTCTC 3 cut(s) 459, 1264, 1551
BsmFI GGGAC 2 cut(s) 513, 864
BsnI GGCC 4 cut(s) 509, 591, 1357, 1661
Bsp1286I GDGCHC 1 cut(s) 143
Bsp143I GATC 4 cut(s) 423, 667, 830, 928
Bsp1720I GCTNAGC 1 cut(s) 107
Bsp68I TCGCGA 1 cut(s) 935
BspACI CCGC 3 cut(s) 525, 980, 1298
BspANI GGCC 4 cut(s) 509, 591, 1357, 1661
BspCNI CTCAG 4 cut(s) 504, 853, 1583, 1674
BspFNI CGCG 1 cut(s) 935
BspHI TCATGA 1 cut(s) 1164
BspLI GGNNCC 4 cut(s) 307, 529, 832, 1020
BspPI GGATC 3 cut(s) 418, 825, 838
BsrDI GCAATG 2 cut(s) 304, 948
BsrI ACTGG 3 cut(s) 309, 1229, 1584
BssECI CCNNGG 3 cut(s) 1285, 1600, 1662
BssMI GATC 4 cut(s) 423, 667, 830, 928
BssT1I CCWWGG 1 cut(s) 1662
Bst2UI CCWGG 1 cut(s) 1602
Bst4CI ACNGT 3 cut(s) 203, 599, 1058
Bst6I CTCTTC 1 cut(s) 815
BstC8I GCNNGC 1 cut(s) 768
BstDSI CCRYGG 1 cut(s) 1285
BstF5I GGATG 3 cut(s) 697, 786, 834
BstFNI CGCG 1 cut(s) 935
BstKTI GATC 4 cut(s) 426, 670, 833, 931
BstMAI GTCTC 3 cut(s) 459, 1264, 1551
BstMBI GATC 4 cut(s) 423, 667, 830, 928
BstMWI GCNNNNNNNGC 3 cut(s) 659, 1131, 1402
BstNI CCWGG 1 cut(s) 1602
BstNSI RCATGY 2 cut(s) 323, 334
BstSCI CCNGG 4 cut(s) 48, 837, 1352, 1600
BstSFI CTRYAG 1 cut(s) 1260
BstUI CGCG 1 cut(s) 935
BstV2I GAAGAC 1 cut(s) 51
BstX2I RGATCY 2 cut(s) 830, 928
BstXI CCANNNNNNTGG 3 cut(s) 1158, 1217, 1591
BstYI RGATCY 2 cut(s) 830, 928
Bsu36I CCTNAGG 2 cut(s) 775, 1682
BsuI GTATCC 1 cut(s) 1607
BsuRI GGCC 4 cut(s) 509, 591, 1357, 1661
BtgI CCRYGG 1 cut(s) 1285
BtsCI GGATG 3 cut(s) 697, 786, 834
BtsIMutI CAGTG 1 cut(s) 973
BtuMI TCGCGA 1 cut(s) 935
Cac8I GCNNGC 1 cut(s) 768
CaiI CAGNNNCTG 1 cut(s) 1056
CciI TCATGA 1 cut(s) 1164
Cfr13I GGNCC 5 cut(s) 4, 289, 527, 599, 1355
Csp6I GTAC 1 cut(s) 799
CviAII CATG 6 cut(s) 320, 331, 343, 403, 1165, 1518
CviQI GTAC 1 cut(s) 799
DpnI GATC 4 cut(s) 425, 669, 832, 930
DpnII GATC 4 cut(s) 423, 667, 830, 928
EaeI YGGCCR 1 cut(s) 507
Eam1104I CTCTTC 1 cut(s) 815
EarI CTCTTC 1 cut(s) 815
Ecl136II GAGCTC 1 cut(s) 141
Eco130I CCWWGG 1 cut(s) 1662
Eco24I GRGCYC 1 cut(s) 143
Eco47I GGWCC 4 cut(s) 4, 289, 527, 599
Eco53kI GAGCTC 1 cut(s) 141
Eco57I CTGAAG 2 cut(s) 63, 933
Eco81I CCTNAGG 2 cut(s) 775, 1682
EcoICRI GAGCTC 1 cut(s) 141
EcoRI GAATTC 2 cut(s) 514, 1505
EcoRII CCWGG 1 cut(s) 1600
EcoT14I CCWWGG 1 cut(s) 1662
EcoT38I GRGCYC 1 cut(s) 143
ErhI CCWWGG 1 cut(s) 1662
FaeI CATG 6 cut(s) 323, 334, 346, 406, 1168, 1521
FalI AAGNNNNNCTT 4 cut(s) 276, 308, 1053, 1085
FaqI GGGAC 2 cut(s) 513, 864
FatI CATG 6 cut(s) 319, 330, 342, 402, 1164, 1517
FauI CCCGC 2 cut(s) 987, 1305
FbaI TGATCA 1 cut(s) 667
FokI GGATG 3 cut(s) 704, 793, 821
FriOI GRGCYC 1 cut(s) 143
FspBI CTAG 8 cut(s) 347, 363, 486, 731, 741, 866, 875, 1106
GsuI CTGGAG 1 cut(s) 1521
HaeIII GGCC 4 cut(s) 509, 591, 1357, 1661
HapII CCGG 3 cut(s) 49, 839, 1353
Hin1II CATG 6 cut(s) 323, 334, 346, 406, 1168, 1521
HincII GTYRAC 2 cut(s) 328, 942
HindII GTYRAC 2 cut(s) 328, 942
HindIII AAGCTT 2 cut(s) 1031, 1223
HinfI GANTC 4 cut(s) 71, 725, 1142, 1435
HpaI GTTAAC 2 cut(s) 328, 942
HpaII CCGG 3 cut(s) 49, 839, 1353
HphI GGTGA 1 cut(s) 1544
Hpy166II GTNNAC 4 cut(s) 289, 328, 942, 1607
Hpy188I TCNGA 5 cut(s) 198, 279, 494, 862, 1141
Hpy8I GTNNAC 4 cut(s) 289, 328, 942, 1607
HpyAV CCTTC 5 cut(s) 348, 392, 399, 602, 996
HpyCH4III ACNGT 3 cut(s) 203, 599, 1058
HpyF10VI GCNNNNNNNGC 3 cut(s) 659, 1131, 1402
Hsp92II CATG 6 cut(s) 323, 334, 346, 406, 1168, 1521
Ksp22I TGATCA 1 cut(s) 667
KspAI GTTAAC 2 cut(s) 328, 942
Kzo9I GATC 4 cut(s) 423, 667, 830, 928
LmnI GCTCC 3 cut(s) 31, 305, 789
LweI GCATC 5 cut(s) 28, 356, 771, 1143, 1414
MaeI CTAG 8 cut(s) 347, 363, 486, 731, 741, 866, 875, 1106
MaeIII GTNAC 5 cut(s) 125, 203, 351, 726, 1255
MalI GATC 4 cut(s) 425, 669, 832, 930
MboI GATC 4 cut(s) 423, 667, 830, 928
MboII GAAGA 6 cut(s) 56, 216, 578, 802, 1167, 1639
MfeI CAATTG 1 cut(s) 1400
MflI RGATCY 2 cut(s) 830, 928
MhlI GDGCHC 1 cut(s) 143
MlsI TGGCCA 1 cut(s) 509
MluNI TGGCCA 1 cut(s) 509
MlyI GAGTC 2 cut(s) 80, 734
MmeI TCCRAC 4 cut(s) 36, 702, 1258, 1534
Mox20I TGGCCA 1 cut(s) 509
MroXI GAANNNNTTC 2 cut(s) 381, 518
MscI TGGCCA 1 cut(s) 509
MseI TTAA 8 cut(s) 327, 450, 477, 549, 585, 908, 941, 1469
MslI CAYNNNNRTG 2 cut(s) 825, 1410
Msp20I TGGCCA 1 cut(s) 509
MspA1I CMGCKG 1 cut(s) 525
MspI CCGG 3 cut(s) 49, 839, 1353
MspR9I CCNGG 4 cut(s) 50, 839, 1354, 1602
MunI CAATTG 1 cut(s) 1400
MvaI CCWGG 1 cut(s) 1602
MvnI CGCG 1 cut(s) 935
MwoI GCNNNNNNNGC 3 cut(s) 659, 1131, 1402
NciI CCSGG 3 cut(s) 50, 839, 1354
NdeII GATC 4 cut(s) 423, 667, 830, 928
NlaIII CATG 6 cut(s) 323, 334, 346, 406, 1168, 1521
NlaIV GGNNCC 4 cut(s) 307, 529, 832, 1020
NmuCI GTSAC 2 cut(s) 726, 1255
NruI TCGCGA 1 cut(s) 935
NspI RCATGY 2 cut(s) 323, 334
PagI TCATGA 1 cut(s) 1164
PciI ACATGT 1 cut(s) 319
PdmI GAANNNNTTC 2 cut(s) 381, 518
PfeI GAWTC 2 cut(s) 1142, 1435
PflMI CCANNNNNTGG 1 cut(s) 1192
PfoI TCCNGGA 1 cut(s) 837
PleI GAGTC 2 cut(s) 79, 733
PpsI GAGTC 2 cut(s) 79, 733
PscI ACATGT 1 cut(s) 319
Psp124BI GAGCTC 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 1600
PspGI CCWGG 1 cut(s) 1600
PspN4I GGNNCC 4 cut(s) 307, 529, 832, 1020
PspPI GGNCC 5 cut(s) 4, 289, 527, 599, 1355
PsrI GAACNNNNNNTAC 4 cut(s) 191, 223, 1588, 1620
PstNI CAGNNNCTG 1 cut(s) 1056
PsuI RGATCY 2 cut(s) 830, 928
RruI TCGCGA 1 cut(s) 935
RsaI GTAC 1 cut(s) 800
RsaNI GTAC 1 cut(s) 799
RseI CAYNNNNRTG 2 cut(s) 825, 1410
SacI GAGCTC 1 cut(s) 143
SaqAI TTAA 8 cut(s) 327, 450, 477, 549, 585, 908, 941, 1469
Sau3AI GATC 4 cut(s) 423, 667, 830, 928
Sau96I GGNCC 5 cut(s) 4, 289, 527, 599, 1355
SchI GAGTC 2 cut(s) 80, 734
ScrFI CCNGG 4 cut(s) 50, 839, 1354, 1602
SduI GDGCHC 1 cut(s) 143
SfaNI GCATC 5 cut(s) 28, 356, 771, 1143, 1414
SfcI CTRYAG 1 cut(s) 1260
SinI GGWCC 4 cut(s) 4, 289, 527, 599
SmiMI CAYNNNNRTG 2 cut(s) 825, 1410
SmlI CTYRAG 2 cut(s) 136, 1512
SmoI CTYRAG 2 cut(s) 136, 1512
SpeI ACTAGT 1 cut(s) 1105
SsiI CCGC 3 cut(s) 525, 980, 1298
SspI AATATT 1 cut(s) 1579
SspMI CTAG 8 cut(s) 347, 363, 486, 731, 741, 866, 875, 1106
SstI GAGCTC 1 cut(s) 143
StyD4I CCNGG 4 cut(s) 48, 837, 1352, 1600
StyI CCWWGG 1 cut(s) 1662
TaaI ACNGT 3 cut(s) 203, 599, 1058
TaqI TCGA 4 cut(s) 143, 813, 1622, 1641
TfiI GAWTC 2 cut(s) 1142, 1435
Tru1I TTAA 8 cut(s) 327, 450, 477, 549, 585, 908, 941, 1469
Tru9I TTAA 8 cut(s) 327, 450, 477, 549, 585, 908, 941, 1469
TscAI CASTG 1 cut(s) 973
TseFI GTSAC 2 cut(s) 726, 1255
Tsp45I GTSAC 2 cut(s) 726, 1255
TspDTI ATGAA 9 cut(s) 297, 419, 535, 860, 1153, 1181, 1335, 1428, 1640
TspRI CASTG 1 cut(s) 973
Van91I CCANNNNNTGG 1 cut(s) 1192
VpaK11BI GGWCC 4 cut(s) 4, 289, 527, 599
XapI RAATTY 9 cut(s) 165, 219, 232, 514, 611, 1212, 1330, 1505, 1637
XbaI TCTAGA 2 cut(s) 362, 874
XceI RCATGY 2 cut(s) 323, 334
XcmI CCANNNNNNNNNTGG 1 cut(s) 465
XmnI GAANNNNTTC 2 cut(s) 381, 518
XspI CTAG 8 cut(s) 347, 363, 486, 731, 741, 866, 875, 1106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.