Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0012192

Physical Location & Seq

Genomic Coordinates Forward (+)

29545203 .. 29550678

Gene Structure 5476 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1215000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1647 bp

ATGCCCACCCGTTCATCATCATCGTCATCTGACTCGGGAGGCGATCAGAGCCCCGAGCTCCGGTCACCTCTGCTCTCCAATGCAAATAAACCCGCCGGAGAGCCTGAATCAGAGAAACTATGCATCGACGACATGTTACAAAAGTATTGCGGGGAGTTTGGTCCGTACCAAATGAAGCACTTTGTGCTGACCAGCTTGGCTTGGGCTCTGGAGGCCTTCCACACCATGGTCATGATATTTGCAGACCGTGAACCGACTTGGCAGTGTTTGGATGGAGCATCTGGTTCGGGTTGCGATGCCAGGGCGAGGACCGTTTGCGGGCTCGAACCGGGTTCGTGGGAGTGGATCGGCGGTCTTGGAAGCTCGACCGTTGCTGAATGGGGGTTGGTTTGTGAGGAGAAGTATAAGGTTGGGCTGGCTCAGGCCTTGTTCTTCGGCGGCTGTATGATTGGTGCGGGAATCTTTGGGCATTTGTCAGACTCGTTTTTAGGAAGAAAAGGCTCCCTAACCGTTGTTTGCATTGTGAATACGATCTTCGGTTGCCTAACAGCATTCTCCCCAAACTACTGGACCTACGTCCTCTTCCGCCTCCTCACCGGCTTCAGCACAGGTGGGGTTGGTCTATGTGCCTTCGTCCTTGCCACTGAACCTGTCGGTCCTACAAAGCGTGGTGCAGCAGGAATGTCCACATTCTACTTCTTCTCAATAGGAATTGCAATCCTCTCTTGTGTTGCGTACGTTTTCCCACAATGGCGCGAGCTCTACATTGTCTCCTCAATCCCATCATTGCTATTCTTGATCGTTGTCCTACCATTTGTCTCTGAGTCCCCAAGGTGGTACCTCATACGAGGAAGAATATCTGAAGCCATGAAACTAATGCGCGTAATTGCAAACTCCAACAAGAACTACCTTCCTGAGGGAGTTGTCCTAACCCTAGACGAAGAAGCAAGCAATGATAATAGTACTACTATTACAAACGAGGATGACCAAACATACAAGGAACTATTGGAGAGCAAAGAAGCAATAAGTGGTTCTCTAATTGATGTGATGAAGTCTCCAGTTACTCGAATTCGGTTATTCCTAGCTGTGGCCATAAATTTCTTGTGCTCGGTTGTGTACTATGGTTTGAGTTTGAACGTTGTCAACCTCGAAACCAACCTCTACATCAATGTGCTACTCAATGCTGTGGCGGAAATGCCAGCTTTCACAATCACAGCAATATTACTGGACAAGTATGGAAGGAAGCCATTGGCAATTGGGACACTATGGTTTAGTGGGGTTTTTTGTTTTCTTGGGAGCTTGGTGGGGAATGTTGGGGTGTGGAAAGTTGTTAGGATGGTGTGCGGGATTTTGGGGATTTTTGGGATGGCGGGGACTTACAATTTGCTTTTTATTTACACCACGGAGTTGTTTCCAACTGTGGTGAGGAATGCAGCGCTTGGATGTGCCACACAAGCCGCACAAATGGGGGCGATTTTGGCCCCGTTTGTGGTGGTTTTGGGCGGGGGTTTGCCGTTTGCAGTGTTTGCCATGTGTGGGGTTGTGGGTGGAATGTTTGCATTTTATTTGCCGGAGACGTTGAATAAGCCTTTGTATGATACGATGACTGGGATGGAGGATGGGGAGGGTGCTTTCAGAAGTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001101 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0007154 GO:0008150 GO:0008509 GO:0008514 GO:0009414 GO:0009415 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009605 GO:0009628 GO:0009705 GO:0009987 GO:0009991 GO:0010035 GO:0015075 GO:0015711 GO:0016020 GO:0016021 GO:0022857 GO:0031090 GO:0031224 GO:0031668 GO:0031976 GO:0031984 GO:0033554 GO:0034220 GO:0034357 GO:0042221 GO:0042631 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044436 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055035 GO:0055085 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071702 GO:0098588 GO:0098656 GO:0098805 GO:0104004 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko05231 map05231

Pfam Domains

Protein Families

Sugar_tr

Protein Analysis

549

Amino Acids

59.31

Weight (kDa)

5.12

Isoelectric Point (pI)

36.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sugar_tr	PF00083	134 - 533	2e-36	Sugar (and other) transporter
MFS_1	PF07690	137 - 280	3.4e-10	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012192)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73220 AT3G20660
fragaria_vesca	FvH4_6g28280
malus_domestica	MD03G1215000.v1.1 MD11G1229700.v1.1
prunus_persica	Prupe.4G197800_v2.0.a1
pyrus_communis	pycom03g16350 pycom11g20340
rosa_chinensis	RchiOBHm_Chr2g0132341
rosa_laevigata	RLG00000019247
rosa_multiflora	Rmu_sc0000781.1_g000003 Rmu_sc0002795.1_g000003
rosa_roxburghii	Rroxscaffold_2G00112150
rosa_rugosa	Rorug02G0303700
rosa_samantha	Rh2AG356800 Rh2BG363300 Rh2CG340400 Rh2DG380100
rosa_wichuraiana	Rw2G029010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	837
AccB1I	GGYRCC	1 cut(s)	837
AccB7I	CCANNNNNTGG	1 cut(s)	226
AccII	CGCG	2 cut(s)	756, 882
AclI	AACGTT	1 cut(s)	1137
AclWI	GGATC	1 cut(s)	353
AcoI	YGGCCR	1 cut(s)	1089
AcsI	RAATTY	2 cut(s)	1068, 1096
AcuI	CTGAAG	2 cut(s)	586, 882
AdeI	CACNNNGTG	1 cut(s)	184
AfaI	GTAC	5 cut(s)	167, 737, 839, 964, 1118
AfeI	AGCGCT	1 cut(s)	1437
AfiI	CCNNNNNNNGG	9 cut(s)	60, 226, 306, 318, 834, 916, 1087, 1489, 1536
AflIII	ACRYGT	1 cut(s)	132
AgsI	TTSAA	2 cut(s)	1135, 1582
AjnI	CCWGG	1 cut(s)	299
AluBI	AGCT	7 cut(s)	58, 195, 363, 760, 1085, 1202, 1299
AluI	AGCT	7 cut(s)	58, 195, 363, 760, 1085, 1202, 1299
Alw21I	GWGCWC	3 cut(s)	60, 762, 1109
Alw26I	GTCTC	4 cut(s)	775, 823, 1059, 1568
AlwI	GGATC	1 cut(s)	353
Ama87I	CYCGRG	2 cut(s)	34, 53
Aor51HI	AGCGCT	1 cut(s)	1437
AoxI	GGCC	4 cut(s)	213, 423, 1089, 1479
ApeKI	GCWGC	2 cut(s)	674, 1433
ApoI	RAATTY	2 cut(s)	1068, 1096
ArsI	GACNNNNNNTTYG	2 cut(s)	1366, 1398
Asp718I	GGTACC	1 cut(s)	837
AspLEI	GCGC	3 cut(s)	756, 882, 1438
AspS9I	GGNCC	5 cut(s)	161, 309, 570, 656, 1480
AsuC2I	CCSGG	1 cut(s)	330
AsuHPI	GGTGA	3 cut(s)	57, 586, 1435
AvaI	CYCGRG	2 cut(s)	34, 53
AvaII	GGWCC	4 cut(s)	161, 309, 570, 656
AxyI	CCTNAGG	1 cut(s)	915
BalI	TGGCCA	1 cut(s)	1091
BanI	GGYRCC	1 cut(s)	837
BanII	GRGCYC	5 cut(s)	53, 60, 208, 324, 762
Bbv12I	GWGCWC	3 cut(s)	60, 762, 1109
BbvI	GCAGC	2 cut(s)	686, 1445
BccI	CCATC	6 cut(s)	266, 790, 1330, 1360, 1606, 1613
BceAI	ACGGC	1 cut(s)	1498
BcgI	CGANNNNNNTGC	2 cut(s)	267, 301
BciT130I	CCWGG	1 cut(s)	301
BcnI	CCSGG	1 cut(s)	330
BcoDI	GTCTC	4 cut(s)	775, 823, 1059, 1568
BfaI	CTAG	2 cut(s)	935, 1082
BfoI	RGCGCY	1 cut(s)	1439
BisI	GCNGC	4 cut(s)	439, 675, 1434, 1458
BlsI	GCNGC	4 cut(s)	440, 676, 1435, 1459
BmcAI	AGTACT	1 cut(s)	964
Bme1390I	CCNGG	2 cut(s)	301, 330
Bme18I	GGWCC	4 cut(s)	161, 309, 570, 656
BmeT110I	CYCGRG	2 cut(s)	34, 53
BmgT120I	GGNCC	5 cut(s)	161, 309, 570, 656, 1480
BmiI	GGNNCC	3 cut(s)	502, 839, 1482
BmrFI	CCNGG	2 cut(s)	301, 330
BmrI	ACTGGG	1 cut(s)	1617
BmsI	GCATC	3 cut(s)	132, 286, 287
BmuI	ACTGGG	1 cut(s)	1617
BoxI	GACNNNNGTC	1 cut(s)	575
BpmI	CTGGAG	2 cut(s)	230, 1041
Bpu10I	CCTNAGC	1 cut(s)	420
BpuMI	CCSGG	1 cut(s)	330
BsaJI	CCNNGG	4 cut(s)	225, 300, 830, 1401
BsaWI	WCCGGW	1 cut(s)	60
BsaXI	ACNNNNNCTCC	2 cut(s)	755, 785
Bsc4I	CCNNNNNNNGG	9 cut(s)	60, 226, 306, 318, 834, 916, 1087, 1489, 1536
Bse118I	RCCGGY	1 cut(s)	596
Bse1I	ACTGG	4 cut(s)	572, 1058, 1230, 1612
Bse21I	CCTNAGG	1 cut(s)	915
Bse3DI	GCAATG	2 cut(s)	785, 958
BseBI	CCWGG	1 cut(s)	301
BseDI	CCNNGG	4 cut(s)	225, 300, 830, 1401
BseGI	GGATG	7 cut(s)	277, 988, 1341, 1371, 1448, 1617, 1624
BseLI	CCNNNNNNNGG	9 cut(s)	60, 226, 306, 318, 834, 916, 1087, 1489, 1536
BseMI	GCAATG	2 cut(s)	785, 958
BseMII	CTCAG	3 cut(s)	434, 813, 906
BseNI	ACTGG	4 cut(s)	572, 1058, 1230, 1612
BseRI	GAGGAG	3 cut(s)	410, 581, 763
BseXI	GCAGC	2 cut(s)	686, 1445
BsgI	GTGCAG	1 cut(s)	693
Bsh1236I	CGCG	2 cut(s)	756, 882
Bsh1285I	CGRYCG	1 cut(s)	369
BshFI	GGCC	4 cut(s)	215, 425, 1091, 1481
BshNI	GGYRCC	1 cut(s)	837
BsiEI	CGRYCG	1 cut(s)	369
BsiHKAI	GWGCWC	3 cut(s)	60, 762, 1109
BsiHKCI	CYCGRG	2 cut(s)	34, 53
BsiSI	CCGG	5 cut(s)	61, 96, 329, 597, 1571
BsiWI	CGTACG	1 cut(s)	735
BslFI	GGGAC	3 cut(s)	811, 1273, 1387
BslI	CCNNNNNNNGG	9 cut(s)	60, 226, 306, 318, 834, 916, 1087, 1489, 1536
BsmAI	GTCTC	4 cut(s)	775, 823, 1059, 1568
BsmBI	CGTCTC	1 cut(s)	1568
BsmFI	GGGAC	3 cut(s)	811, 1273, 1387
BsmI	GAATGC	2 cut(s)	551, 1435
BsnI	GGCC	4 cut(s)	215, 425, 1091, 1481
BsoBI	CYCGRG	2 cut(s)	34, 53
Bsp1286I	GDGCHC	6 cut(s)	53, 60, 208, 324, 762, 1109
Bsp143I	GATC	4 cut(s)	43, 345, 531, 798
Bsp19I	CCATGG	1 cut(s)	225
BspANI	GGCC	4 cut(s)	215, 425, 1091, 1481
BspCNI	CTCAG	3 cut(s)	433, 814, 907
BspFNI	CGCG	2 cut(s)	756, 882
BspHI	TCATGA	1 cut(s)	231
BspLI	GGNNCC	3 cut(s)	502, 839, 1482
BspPI	GGATC	1 cut(s)	353
BspT107I	GGYRCC	1 cut(s)	837
BsrDI	GCAATG	2 cut(s)	785, 958
BsrFI	RCCGGY	1 cut(s)	596
BsrI	ACTGG	4 cut(s)	572, 1058, 1230, 1612
BssAI	RCCGGY	1 cut(s)	596
BssECI	CCNNGG	4 cut(s)	225, 300, 830, 1401
BssMI	GATC	4 cut(s)	43, 345, 531, 798
BssT1I	CCWWGG	2 cut(s)	225, 830
Bst2UI	CCWGG	1 cut(s)	301
Bst4CI	ACNGT	5 cut(s)	248, 313, 370, 511, 1420
Bst6I	CTCTTC	1 cut(s)	587
BstAPI	GCANNNNNTGC	2 cut(s)	184, 1526
BstC8I	GCNNGC	5 cut(s)	320, 417, 758, 949, 1200
BstDEI	CTNAG	3 cut(s)	420, 822, 915
BstDSI	CCRYGG	2 cut(s)	225, 1401
BstEII	GGTNACC	1 cut(s)	63
BstENI	CCTNNNNNAGG	1 cut(s)	914
BstF5I	GGATG	7 cut(s)	277, 988, 1341, 1371, 1448, 1617, 1624
BstFNI	CGCG	2 cut(s)	756, 882
BstH2I	RGCGCY	1 cut(s)	1439
BstHHI	GCGC	3 cut(s)	756, 882, 1438
BstKTI	GATC	4 cut(s)	46, 348, 534, 801
BstMAI	GTCTC	4 cut(s)	775, 823, 1059, 1568
BstMBI	GATC	4 cut(s)	43, 345, 531, 798
BstMCI	CGRYCG	1 cut(s)	369
BstMWI	GCNNNNNNNGC	6 cut(s)	48, 184, 212, 1454, 1478, 1526
BstNI	CCWGG	1 cut(s)	301
BstNSI	RCATGY	1 cut(s)	136
BstPAI	GACNNNNGTC	1 cut(s)	575
BstPI	GGTNACC	1 cut(s)	63
BstSCI	CCNGG	2 cut(s)	299, 328
BstUI	CGCG	2 cut(s)	756, 882
BstV1I	GCAGC	2 cut(s)	686, 1445
BstXI	CCANNNNNNTGG	1 cut(s)	567
Bsu36I	CCTNAGG	1 cut(s)	915
BsuRI	GGCC	4 cut(s)	215, 425, 1091, 1481
BtgI	CCRYGG	2 cut(s)	225, 1401
BtgZI	GCGATG	1 cut(s)	309
BtsCI	GGATG	7 cut(s)	277, 988, 1341, 1371, 1448, 1617, 1624
BtsI	GCAGTG	2 cut(s)	269, 1527
BtsIMutI	CAGTG	3 cut(s)	269, 642, 1527
Cac8I	GCNNGC	5 cut(s)	320, 417, 758, 949, 1200
CciI	TCATGA	1 cut(s)	231
CfoI	GCGC	3 cut(s)	756, 882, 1438
Cfr10I	RCCGGY	1 cut(s)	596
Cfr13I	GGNCC	5 cut(s)	161, 309, 570, 656, 1480
Csp6I	GTAC	5 cut(s)	166, 736, 838, 963, 1117
CviAII	CATG	5 cut(s)	133, 226, 232, 868, 1531
CviQI	GTAC	5 cut(s)	166, 736, 838, 963, 1117
DdeI	CTNAG	3 cut(s)	420, 822, 915
DpnI	GATC	4 cut(s)	45, 347, 533, 800
DpnII	GATC	4 cut(s)	43, 345, 531, 798
DraIII	CACNNNGTG	1 cut(s)	184
EaeI	YGGCCR	1 cut(s)	1089
Eam1104I	CTCTTC	1 cut(s)	587
EarI	CTCTTC	1 cut(s)	587
EciI	GGCGGA	2 cut(s)	575, 1205
Ecl136II	GAGCTC	2 cut(s)	58, 760
Eco130I	CCWWGG	2 cut(s)	225, 830
Eco147I	AGGCCT	2 cut(s)	215, 425
Eco24I	GRGCYC	5 cut(s)	53, 60, 208, 324, 762
Eco47I	GGWCC	4 cut(s)	161, 309, 570, 656
Eco47III	AGCGCT	1 cut(s)	1437
Eco53kI	GAGCTC	2 cut(s)	58, 760
Eco57I	CTGAAG	2 cut(s)	586, 882
Eco81I	CCTNAGG	1 cut(s)	915
Eco88I	CYCGRG	2 cut(s)	34, 53
Eco91I	GGTNACC	1 cut(s)	63
EcoICRI	GAGCTC	2 cut(s)	58, 760
EcoNI	CCTNNNNNAGG	1 cut(s)	914
EcoO65I	GGTNACC	1 cut(s)	63
EcoRI	GAATTC	1 cut(s)	1068
EcoRII	CCWGG	1 cut(s)	299
EcoT14I	CCWWGG	2 cut(s)	225, 830
EcoT22I	ATGCAT	1 cut(s)	125
EcoT38I	GRGCYC	5 cut(s)	53, 60, 208, 324, 762
ErhI	CCWWGG	2 cut(s)	225, 830
Esp3I	CGTCTC	1 cut(s)	1568
FaeI	CATG	5 cut(s)	136, 229, 235, 871, 1534
FaqI	GGGAC	3 cut(s)	811, 1273, 1387
FatI	CATG	5 cut(s)	132, 225, 231, 867, 1530
FauI	CCCGC	7 cut(s)	100, 143, 311, 448, 1337, 1363, 1496
Fnu4HI	GCNGC	4 cut(s)	439, 675, 1434, 1458
FokI	GGATG	7 cut(s)	284, 995, 1348, 1378, 1455, 1624, 1631
FriOI	GRGCYC	5 cut(s)	53, 60, 208, 324, 762
Fsp4HI	GCNGC	4 cut(s)	439, 675, 1434, 1458
FspBI	CTAG	2 cut(s)	935, 1082
GlaI	GCGC	3 cut(s)	755, 881, 1437
GluI	GCNGC	4 cut(s)	439, 675, 1434, 1458
GsuI	CTGGAG	2 cut(s)	230, 1041
HaeII	RGCGCY	1 cut(s)	1439
HaeIII	GGCC	4 cut(s)	215, 425, 1091, 1481
HapII	CCGG	5 cut(s)	61, 96, 329, 597, 1571
HhaI	GCGC	3 cut(s)	756, 882, 1438
Hin1II	CATG	5 cut(s)	136, 229, 235, 871, 1534
Hin6I	GCGC	3 cut(s)	754, 880, 1436
HinP1I	GCGC	3 cut(s)	754, 880, 1436
HincII	GTYRAC	1 cut(s)	1144
HindII	GTYRAC	1 cut(s)	1144
HinfI	GANTC	5 cut(s)	32, 107, 459, 479, 824
HpaII	CCGG	5 cut(s)	61, 96, 329, 597, 1571
HphI	GGTGA	3 cut(s)	57, 586, 1435
Hpy166II	GTNNAC	4 cut(s)	251, 687, 1117, 1144
Hpy188I	TCNGA	7 cut(s)	31, 48, 112, 478, 823, 862, 1637
Hpy188III	TCNNGA	5 cut(s)	36, 209, 232, 796, 914
Hpy8I	GTNNAC	4 cut(s)	251, 687, 1117, 1144
Hpy99I	CGWCG	1 cut(s)	131
HpyAV	CCTTC	4 cut(s)	226, 640, 920, 1233
HpyCH4III	ACNGT	5 cut(s)	248, 313, 370, 511, 1420
HpyCH4IV	ACGT	4 cut(s)	576, 738, 1137, 1577
HpyF10VI	GCNNNNNNNGC	6 cut(s)	48, 184, 212, 1454, 1478, 1526
HpyF3I	CTNAG	3 cut(s)	420, 822, 915
HpySE526I	ACGT	4 cut(s)	576, 738, 1137, 1577
Hsp92II	CATG	5 cut(s)	136, 229, 235, 871, 1534
HspAI	GCGC	3 cut(s)	754, 880, 1436
KpnI	GGTACC	1 cut(s)	841
Kzo9I	GATC	4 cut(s)	43, 345, 531, 798
LmnI	GCTCC	4 cut(s)	63, 275, 506, 1296
Lsp1109I	GCAGC	2 cut(s)	686, 1445
LweI	GCATC	3 cut(s)	132, 286, 287
MaeI	CTAG	2 cut(s)	935, 1082
MaeII	ACGT	4 cut(s)	576, 738, 1137, 1577
MaeIII	GTNAC	3 cut(s)	63, 135, 1060
MalI	GATC	4 cut(s)	45, 347, 533, 800
MboI	GATC	4 cut(s)	43, 345, 531, 798
MboII	GAAGA	7 cut(s)	424, 504, 526, 574, 691, 864, 953
MfeI	CAATTG	1 cut(s)	1254
MhlI	GDGCHC	6 cut(s)	53, 60, 208, 324, 762, 1109
MlsI	TGGCCA	1 cut(s)	1091
MluCI	AATT	7 cut(s)	711, 885, 1038, 1068, 1096, 1254, 1381
MluNI	TGGCCA	1 cut(s)	1091
MlyI	GAGTC	3 cut(s)	26, 473, 833
MmeI	TCCRAC	2 cut(s)	921, 1439
Mox20I	TGGCCA	1 cut(s)	1091
Mph1103I	ATGCAT	1 cut(s)	125
MscI	TGGCCA	1 cut(s)	1091
MslI	CAYNNNNRTG	2 cut(s)	230, 1169
Msp20I	TGGCCA	1 cut(s)	1091
MspI	CCGG	5 cut(s)	61, 96, 329, 597, 1571
MspR9I	CCNGG	2 cut(s)	301, 330
MunI	CAATTG	1 cut(s)	1254
Mva1269I	GAATGC	2 cut(s)	551, 1435
MvaI	CCWGG	1 cut(s)	301
MvnI	CGCG	2 cut(s)	756, 882
MwoI	GCNNNNNNNGC	6 cut(s)	48, 184, 212, 1454, 1478, 1526
NciI	CCSGG	1 cut(s)	330
NcoI	CCATGG	1 cut(s)	225
NdeII	GATC	4 cut(s)	43, 345, 531, 798
NlaIII	CATG	5 cut(s)	136, 229, 235, 871, 1534
NlaIV	GGNNCC	3 cut(s)	502, 839, 1482
NmuCI	GTSAC	1 cut(s)	63
NsiI	ATGCAT	1 cut(s)	125
NspI	RCATGY	1 cut(s)	136
PagI	TCATGA	1 cut(s)	231
PceI	AGGCCT	2 cut(s)	215, 425
PciI	ACATGT	1 cut(s)	132
PctI	GAATGC	2 cut(s)	551, 1435
PfeI	GAWTC	2 cut(s)	107, 459
Pfl23II	CGTACG	1 cut(s)	735
PflMI	CCANNNNNTGG	1 cut(s)	226
PkrI	GCNGC	4 cut(s)	440, 676, 1435, 1459
PleI	GAGTC	3 cut(s)	26, 473, 832
PpsI	GAGTC	3 cut(s)	26, 473, 832
PscI	ACATGT	1 cut(s)	132
PshAI	GACNNNNGTC	1 cut(s)	575
Psp124BI	GAGCTC	2 cut(s)	60, 762
Psp1406I	AACGTT	1 cut(s)	1137
Psp6I	CCWGG	1 cut(s)	299
PspEI	GGTNACC	1 cut(s)	63
PspGI	CCWGG	1 cut(s)	299
PspLI	CGTACG	1 cut(s)	735
PspN4I	GGNNCC	3 cut(s)	502, 839, 1482
PspPI	GGNCC	5 cut(s)	161, 309, 570, 656, 1480
RsaI	GTAC	5 cut(s)	167, 737, 839, 964, 1118
RsaNI	GTAC	5 cut(s)	166, 736, 838, 963, 1117
RseI	CAYNNNNRTG	2 cut(s)	230, 1169
SacI	GAGCTC	2 cut(s)	60, 762
SatI	GCNGC	4 cut(s)	439, 675, 1434, 1458
Sau3AI	GATC	4 cut(s)	43, 345, 531, 798
Sau96I	GGNCC	5 cut(s)	161, 309, 570, 656, 1480
ScaI	AGTACT	1 cut(s)	964
SchI	GAGTC	3 cut(s)	26, 473, 833
ScrFI	CCNGG	2 cut(s)	301, 330
SduI	GDGCHC	6 cut(s)	53, 60, 208, 324, 762, 1109
SfaNI	GCATC	3 cut(s)	132, 286, 287
SinI	GGWCC	4 cut(s)	161, 309, 570, 656
SmiMI	CAYNNNNRTG	2 cut(s)	230, 1169
Sse9I	AATT	7 cut(s)	711, 885, 1038, 1068, 1096, 1254, 1381
SseBI	AGGCCT	2 cut(s)	215, 425
SspI	AATATT	1 cut(s)	1221
SspMI	CTAG	2 cut(s)	935, 1082
SstI	GAGCTC	2 cut(s)	60, 762
StuI	AGGCCT	2 cut(s)	215, 425
StyD4I	CCNGG	2 cut(s)	299, 328
StyI	CCWWGG	2 cut(s)	225, 830
TaaI	ACNGT	5 cut(s)	248, 313, 370, 511, 1420
TaiI	ACGT	4 cut(s)	579, 741, 1140, 1580
TaqI	TCGA	5 cut(s)	126, 324, 365, 1066, 1149
TaqII	GACCGA	1 cut(s)	644
TasI	AATT	7 cut(s)	711, 885, 1038, 1068, 1096, 1254, 1381
TatI	WGTACW	2 cut(s)	962, 1116
TauI	GCSGC	2 cut(s)	441, 1460
TfiI	GAWTC	2 cut(s)	107, 459
TscAI	CASTG	3 cut(s)	269, 649, 1527
TseFI	GTSAC	1 cut(s)	63
TseI	GCWGC	2 cut(s)	674, 1433
Tsp45I	GTSAC	1 cut(s)	63
TspDTI	ATGAA	3 cut(s)	188, 884, 1064
TspGWI	ACGGA	2 cut(s)	153, 1418
TspRI	CASTG	3 cut(s)	269, 649, 1527
Van91I	CCANNNNNTGG	1 cut(s)	226
VpaK11BI	GGWCC	4 cut(s)	161, 309, 570, 656
XagI	CCTNNNNNAGG	1 cut(s)	914
XapI	RAATTY	2 cut(s)	1068, 1096
XceI	RCATGY	1 cut(s)	136
XspI	CTAG	2 cut(s)	935, 1082
ZrmI	AGTACT	1 cut(s)	964
Zsp2I	ATGCAT	1 cut(s)	125

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.