Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0007973

Physical Location & Seq

Genomic Coordinates Forward (+)

30251311 .. 30259225

Gene Structure 7915 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1218100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2169 bp

ATGGGTGACTTGTCAGGGGAGGATTTCAATGATACGCTTCGAATACTTGTTGCTACGGATTGCCATCTGGGTTATATGGAGAAGGATGAAATACGGCGGCATGATTCATTCCAGGCATTTGATGAGATATGCTCAATAGCGGAACGAAAAAATGTGGATTTTTTGCTTCTTGGTGGTGATCTGTTCCATGAGAATAAGCCTTCAAGGTCAACACTAGTTAAGGTCATTGAGATTCTCCGTCGTCATTGCCTCAATGATCAGCCAGTGCAGTTCCAAGTTGTTAGTGACCAGACTATGAATTTTCCAAATACATTTGGTCATGTAAATTATGAAGATCCTCACTTCAATGTTGGATTGCCAGTATTTAGTATTCATGGAAATCATGACGACCCTGCTGGAGTGGATAATCTTTCTGCTGTCGATATTCTCTCAGCGTGCAACCTTGTAAACTATTTTGGGAAAATGGGTCTTGGGGGTTCTGGTGTTGGTCAGATCACTGTGTATCCTATTCTCATGAGGAAGGGTGCAACATCAGTGGCTCTCTATGGTCTCGGAAATATTAGAGATGAGCGGCTCAATAGAATGTTTCAGACACCACATGCTGTACAATGGATGAGACCTGAATCTCAAGAAGGGTTGGAAGTATCAGACTGGTTCAACATTCTAGTGCTTCATCAGAACAGAGTGAAGACAAATCCAAAAAATGCCATAAATGAGCACTTTTTACCACGCTTCCTAGACTTCGTAGTGTGGGGGCATGAACATGAATGCCTTGTGGATCCTCAGGAAGTTCCAGGGATGGGATTTCACATTACACAACCTGGTTCTTCAGTTGCAACATCACTGATCGATGGAGAATCAAAGCCAAAGCATGTGCTCCTTTTAGAAATTAAGGGGAATCAATACCGTCCAACCAAGATTCCTTTGACGTCTGTGAGGCCGTTTGAGTACACAGAGATTGTATTAAAAGATGAACCTGAAATTGATCCCAATGATCCAAACTCAATTCTTGAACACTTGGACAAAGTGGTCAACTCTCTGATAGAGAAATCTAGGAAAAATGTTGTTAGCGGATCACCGATCCAACTTCCATTGGTCCGAATAAAGGTGGACTATTCTGGATTTATGACAATAAATCCTCAAAGATTTGGGCAGAAGTATGTGGGGAAGGTTGCAAACCCACAAGACATTCTTATATTTTCCAAGGCTTCAACAAAGGGCCGGAGTGAAGCTAAAATTGATGATTCTGAACGGCTTCGCCCGGAAGAACTAAATCAGCAAAACATAGAGGCTTTGGTTGCTGAAAATAATCTGAAAATGGAGATCCTTCCTGTCAATGATTTGGACGTTGCATTACACAATTTTGTTAGTAAGGATGACAAAATGGCTTTTTATTCTTGCGTGAAATACAATCTTGAAGAAACTCAAAAGAAAATTGCTAAGGATCCAGATAATGTAACATTTGAAGAGGAAGATTTAATCGTTAAAGTCGGAGAGTGTTTGCAGGAACGTGTCAAGGAAAGGCCTACGCAGTCTAAGAAAAACTCTCCATTCTCCTCCACTGCCCCTCCTTGGGAGGATTTCAGAGGTACAAGTGCTGCTGGAACTGCAAGTGCAGTTTCCTTCAGTGACGATGAAGATGCAATGCAGATGACTGGCTCCACATCAACTGCCAGAGGCCGCAACGGCAGGATGTCTACCTCAGAAGTTGGTAAGGGTAAAACTACAAGAAGAGGAAGAGGAAGGGGCAGGGGCAGGGGCACCAGTACCTTGAAGCAGTTGACTCTCGATGCATCTCTTGGATTTCGGCATTCTCAGAGATCTGCATCAGTTGCTGCAACTGCCGCTGTTCAAAGTATTGCGGATGATGGAGATGATGTGGATTCTCCAAATGAAGGGTCGGGGAAACTTGGAATTGATGAGGTTGACAACAGTTCGGAAAATGATGAAATTCTTCCACCAAGTAGAGGACGGAAAAGGGCTGCTCCAAGGGGGAGAGGTAGAGGCTCTACACAACCATCTAAACGTGGAAAGAAATCAGATAGCTCTGCAGTTAACCGAATGTTTATGAACAAAGATGATGATGATGATGATGATGACGATGTGACAAAGAGAGTAAACAAGCCTCTGCCTCAGGTCACGAGAAATTATGGCTCTCTTAGAAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000791 GO:0000793 GO:0000794 GO:0000795 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0070013 GO:0099086

KEGG Pathways

Metabolic & Signaling

ko03440 ko03450 ko04218 map03440 map03450 map04218

Pfam Domains

Protein Families

Metallophos Mre11_DNA_bind

Protein Analysis

723

Amino Acids

80.38

Weight (kDa)

5.85

Isoelectric Point (pI)

45.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Metallophos	PF00149	13 - 257	3.3e-15	Calcineurin-like phosphoesterase
Mre11_DNA_bind	PF04152	303 - 455	4.9e-34	Mre11 DNA-binding presumed domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54260 AT5G54260
fragaria_vesca	FvH4_3g21040 FvH4_3g21040 FvH4_3g21040
malus_domestica	MD03G1218100.v1.1 MD11G1235900.v1.1
prunus_persica	Prupe.4G191100_v2.0.a1 Prupe.4G191100_v2.0.a1 Prupe.4G191100_v2.0.a1 Prupe.4G191100_v2.0.a1 Prupe.4G191100_v2.0.a1
pyrus_communis	pycom03g16760
rosa_chinensis	RchiOBHm_Chr5g0036071
rosa_laevigata	RLG00000033663
rosa_multiflora	Rmu_sc0000382.1_g000032
rosa_roxburghii	Rroxscaffold_1G00044680
rosa_rugosa	Rorug05G0152500 Rorug05G0152500
rosa_samantha	Rh5AG246700 Rh5BG247000 Rh5CG278100 Rh5DG255600
rosa_wichuraiana	Rw5G022640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1028
AatII	GACGTC	1 cut(s)	932
AccB1I	GGYRCC	1 cut(s)	1760
AccBSI	CCGCTC	1 cut(s)	571
AccI	GTMKAC	1 cut(s)	1697
AciI	CCGC	7 cut(s)	97, 140, 571, 1071, 1681, 1845, 1862
AcsI	RAATTY	2 cut(s)	298, 1950
AcuI	CTGAAG	2 cut(s)	813, 1609
AcyI	GRCGYC	1 cut(s)	929
AfaI	GTAC	4 cut(s)	606, 950, 1591, 1768
AfiI	CCNNNNNNNGG	6 cut(s)	800, 1105, 1572, 1573, 1895, 1967
AflIII	ACRYGT	1 cut(s)	1510
AhlI	ACTAGT	1 cut(s)	214
AjnI	CCWGG	3 cut(s)	111, 793, 820
AluBI	AGCT	2 cut(s)	1232, 2046
AluI	AGCT	2 cut(s)	1232, 2046
Alw21I	GWGCWC	2 cut(s)	720, 879
Alw26I	GTCTC	2 cut(s)	554, 610
AlwNI	CAGNNNCTG	1 cut(s)	1835
AoxI	GGCC	4 cut(s)	938, 1219, 1523, 1678
ApeKI	GCWGC	3 cut(s)	1598, 1835, 1982
ApoI	RAATTY	2 cut(s)	298, 1950
ArsI	GACNNNNNNTTYG	2 cut(s)	1883, 1915
Asp700I	GAANNNNTTC	2 cut(s)	1254, 1953
AspS9I	GGNCC	2 cut(s)	1096, 1219
AsuC2I	CCSGG	1 cut(s)	1262
AsuHPI	GGTGA	3 cut(s)	17, 188, 1068
AsuII	TTCGAA	1 cut(s)	40
AvaII	GGWCC	1 cut(s)	1096
AxyI	CCTNAGG	2 cut(s)	783, 2133
BaeGI	GKGCMC	1 cut(s)	1763
BamHI	GGATCC	2 cut(s)	778, 1444
BanI	GGYRCC	1 cut(s)	1760
BauI	CACGAG	1 cut(s)	2140
BbsI	GAAGAC	1 cut(s)	695
Bbv12I	GWGCWC	2 cut(s)	720, 879
BbvI	GCAGC	3 cut(s)	1585, 1822, 1969
BccI	CCATC	5 cut(s)	72, 793, 845, 1862, 2026
BceAI	ACGGC	4 cut(s)	110, 925, 1268, 1702
BcgI	CGANNNNNNTGC	2 cut(s)	1622, 1656
BciT130I	CCWGG	3 cut(s)	113, 795, 822
BciVI	GTATCC	1 cut(s)	513
BclI	TGATCA	1 cut(s)	256
BcnI	CCSGG	1 cut(s)	1262
BcoDI	GTCTC	2 cut(s)	554, 610
BcuI	ACTAGT	1 cut(s)	214
BfaI	CTAG	4 cut(s)	215, 665, 737, 1053
BfmI	CTRYAG	1 cut(s)	2049
BfuI	GTATCC	1 cut(s)	513
BglI	GCCNNNNNGGC	1 cut(s)	1686
BglII	AGATCT	1 cut(s)	1820
BisI	GCNGC	7 cut(s)	98, 572, 1599, 1681, 1836, 1845, 1983
BlsI	GCNGC	7 cut(s)	99, 573, 1600, 1682, 1837, 1846, 1984
Bme1390I	CCNGG	4 cut(s)	113, 795, 822, 1262
Bme18I	GGWCC	1 cut(s)	1096
BmgT120I	GGNCC	2 cut(s)	1096, 1219
BmiI	GGNNCC	4 cut(s)	780, 1446, 1660, 1762
BmrFI	CCNGG	4 cut(s)	113, 795, 822, 1262
BmsI	GCATC	4 cut(s)	1630, 1780, 1802, 1835
BpiI	GAAGAC	1 cut(s)	695
BplI	GAGNNNNNCTC	2 cut(s)	116, 148
BpmI	CTGGAG	1 cut(s)	417
Bpu10I	CCTNAGC	1 cut(s)	1440
Bpu14I	TTCGAA	1 cut(s)	40
BpuEI	CTTGAG	1 cut(s)	612
BpuMI	CCSGG	1 cut(s)	1262
Bsa29I	ATCGAT	1 cut(s)	849
BsaBI	GATNNNNATC	2 cut(s)	63, 1825
BsaHI	GRCGYC	1 cut(s)	929
BsaI	GGTCTC	2 cut(s)	554, 610
BsaJI	CCNNGG	4 cut(s)	794, 1203, 1571, 1988
BsaXI	ACNNNNNCTCC	4 cut(s)	1552, 1582, 1863, 1893
Bsc4I	CCNNNNNNNGG	6 cut(s)	800, 1105, 1572, 1573, 1895, 1967
Bse1I	ACTGG	5 cut(s)	263, 359, 656, 1660, 1764
Bse21I	CCTNAGG	2 cut(s)	783, 2133
Bse3DI	GCAATG	2 cut(s)	244, 1650
Bse8I	GATNNNNATC	2 cut(s)	63, 1825
BseBI	CCWGG	3 cut(s)	113, 795, 822
BseCI	ATCGAT	1 cut(s)	849
BseDI	CCNNGG	4 cut(s)	794, 1203, 1571, 1988
BseGI	GGATG	6 cut(s)	91, 618, 804, 1381, 1698, 1870
BseJI	GATNNNNATC	2 cut(s)	63, 1825
BseLI	CCNNNNNNNGG	6 cut(s)	800, 1105, 1572, 1573, 1895, 1967
BseMI	GCAATG	2 cut(s)	244, 1650
BseMII	CTCAG	5 cut(s)	444, 797, 1716, 1829, 2147
BseNI	ACTGG	5 cut(s)	263, 359, 656, 1660, 1764
BseRI	GAGGAG	1 cut(s)	1546
BseSI	GKGCMC	1 cut(s)	1763
BseXI	GCAGC	3 cut(s)	1585, 1822, 1969
BsgI	GTGCAG	2 cut(s)	287, 1635
BshFI	GGCC	4 cut(s)	940, 1221, 1525, 1680
BshNI	GGYRCC	1 cut(s)	1760
BshVI	ATCGAT	1 cut(s)	849
BsiHKAI	GWGCWC	2 cut(s)	720, 879
BsiSI	CCGG	2 cut(s)	1222, 1262
BslI	CCNNNNNNNGG	6 cut(s)	800, 1105, 1572, 1573, 1895, 1967
BsmAI	GTCTC	2 cut(s)	554, 610
BsmI	GAATGC	2 cut(s)	773, 1810
BsnI	GGCC	4 cut(s)	940, 1221, 1525, 1680
Bso31I	GGTCTC	2 cut(s)	554, 610
Bsp119I	TTCGAA	1 cut(s)	40
Bsp1286I	GDGCHC	3 cut(s)	720, 879, 1763
Bsp1407I	TGTACA	1 cut(s)	604
BspACI	CCGC	7 cut(s)	97, 140, 571, 1071, 1681, 1845, 1862
BspANI	GGCC	4 cut(s)	940, 1221, 1525, 1680
BspCNI	CTCAG	5 cut(s)	443, 796, 1715, 1828, 2146
BspDI	ATCGAT	1 cut(s)	849
BspHI	TCATGA	2 cut(s)	382, 513
BspLI	GGNNCC	4 cut(s)	780, 1446, 1660, 1762
BspMAI	CTGCAG	1 cut(s)	2053
BspT104I	TTCGAA	1 cut(s)	40
BspT107I	GGYRCC	1 cut(s)	1760
BspTNI	GGTCTC	2 cut(s)	554, 610
BsrBI	CCGCTC	1 cut(s)	571
BsrDI	GCAATG	2 cut(s)	244, 1650
BsrGI	TGTACA	1 cut(s)	604
BsrI	ACTGG	5 cut(s)	263, 359, 656, 1660, 1764
BssECI	CCNNGG	4 cut(s)	794, 1203, 1571, 1988
BssNI	GRCGYC	1 cut(s)	929
BssSI	CACGAG	1 cut(s)	2140
BssT1I	CCWWGG	3 cut(s)	1203, 1571, 1988
Bst2BI	CACGAG	1 cut(s)	2140
Bst2UI	CCWGG	3 cut(s)	113, 795, 822
Bst4CI	ACNGT	3 cut(s)	499, 908, 1934
Bst6I	CTCTTC	3 cut(s)	1461, 1726, 1732
BstACI	GRCGYC	1 cut(s)	929
BstAPI	GCANNNNNTGC	1 cut(s)	1832
BstAUI	TGTACA	1 cut(s)	604
BstBI	TTCGAA	1 cut(s)	40
BstC8I	GCNNGC	1 cut(s)	436
BstDEI	CTNAG	8 cut(s)	430, 783, 1440, 1536, 1702, 1815, 2133, 2159
BstF5I	GGATG	6 cut(s)	91, 618, 804, 1381, 1698, 1870
BstMAI	GTCTC	2 cut(s)	554, 610
BstMWI	GCNNNNNNNGC	6 cut(s)	1298, 1607, 1686, 1832, 1841, 1844
BstNI	CCWGG	3 cut(s)	113, 795, 822
BstNSI	RCATGY	2 cut(s)	602, 875
BstSCI	CCNGG	4 cut(s)	111, 793, 820, 1260
BstSFI	CTRYAG	1 cut(s)	2049
BstSLI	GKGCMC	1 cut(s)	1763
BstV1I	GCAGC	3 cut(s)	1585, 1822, 1969
BstV2I	GAAGAC	1 cut(s)	695
BstX2I	RGATCY	5 cut(s)	334, 778, 1323, 1444, 1820
BstYI	RGATCY	5 cut(s)	334, 778, 1323, 1444, 1820
Bsu15I	ATCGAT	1 cut(s)	849
Bsu36I	CCTNAGG	2 cut(s)	783, 2133
BsuI	GTATCC	1 cut(s)	513
BsuRI	GGCC	4 cut(s)	940, 1221, 1525, 1680
BsuTUI	ATCGAT	1 cut(s)	849
BtsCI	GGATG	6 cut(s)	91, 618, 804, 1381, 1698, 1870
BtsI	GCAGTG	1 cut(s)	1560
BtsIMutI	CAGTG	6 cut(s)	270, 495, 540, 842, 1560, 1633
Cac8I	GCNNGC	1 cut(s)	436
CaiI	CAGNNNCTG	1 cut(s)	1835
CciI	TCATGA	2 cut(s)	382, 513
Cfr13I	GGNCC	2 cut(s)	1096, 1219
ClaI	ATCGAT	1 cut(s)	849
CsiI	ACCWGGT	1 cut(s)	820
Csp6I	GTAC	4 cut(s)	605, 949, 1590, 1767
CspCI	CAANNNNNGTGG	2 cut(s)	516, 551
CviQI	GTAC	4 cut(s)	605, 949, 1590, 1767
DdeI	CTNAG	8 cut(s)	430, 783, 1440, 1536, 1702, 1815, 2133, 2159
DrdI	GACNNNNNNGTC	1 cut(s)	1028
DseDI	GACNNNNNNGTC	1 cut(s)	1028
Eam1104I	CTCTTC	3 cut(s)	1461, 1726, 1732
EarI	CTCTTC	3 cut(s)	1461, 1726, 1732
Eco130I	CCWWGG	3 cut(s)	1203, 1571, 1988
Eco147I	AGGCCT	1 cut(s)	1525
Eco31I	GGTCTC	2 cut(s)	554, 610
Eco47I	GGWCC	1 cut(s)	1096
Eco57I	CTGAAG	2 cut(s)	813, 1609
Eco81I	CCTNAGG	2 cut(s)	783, 2133
EcoRII	CCWGG	3 cut(s)	111, 793, 820
EcoT14I	CCWWGG	3 cut(s)	1203, 1571, 1988
EcoT22I	ATGCAT	1 cut(s)	1795
ErhI	CCWWGG	3 cut(s)	1203, 1571, 1988
FalI	AAGNNNNNCTT	2 cut(s)	1176, 1208
FbaI	TGATCA	1 cut(s)	256
FblI	GTMKAC	1 cut(s)	1697
Fnu4HI	GCNGC	7 cut(s)	98, 572, 1599, 1681, 1836, 1845, 1983
FokI	GGATG	6 cut(s)	98, 625, 811, 1388, 1705, 1877
Fsp4HI	GCNGC	7 cut(s)	98, 572, 1599, 1681, 1836, 1845, 1983
FspBI	CTAG	4 cut(s)	215, 665, 737, 1053
GluI	GCNGC	7 cut(s)	98, 572, 1599, 1681, 1836, 1845, 1983
GsuI	CTGGAG	1 cut(s)	417
HaeIII	GGCC	4 cut(s)	940, 1221, 1525, 1680
HapII	CCGG	2 cut(s)	1222, 1262
Hin1I	GRCGYC	1 cut(s)	929
HincII	GTYRAC	5 cut(s)	210, 1033, 1782, 1927, 2056
HindII	GTYRAC	5 cut(s)	210, 1033, 1782, 1927, 2056
HinfI	GANTC	9 cut(s)	104, 232, 623, 857, 898, 919, 1244, 1783, 1883
HpaI	GTTAAC	1 cut(s)	2056
HpaII	CCGG	2 cut(s)	1222, 1262
HphI	GGTGA	3 cut(s)	17, 188, 1068
Hpy99I	CGWCG	1 cut(s)	243
HpyCH4III	ACNGT	3 cut(s)	499, 908, 1934
HpyCH4IV	ACGT	4 cut(s)	929, 1347, 1510, 2026
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1298, 1607, 1686, 1832, 1841, 1844
HpyF3I	CTNAG	8 cut(s)	430, 783, 1440, 1536, 1702, 1815, 2133, 2159
HpySE526I	ACGT	4 cut(s)	929, 1347, 1510, 2026
Hsp92I	GRCGYC	1 cut(s)	929
Ksp22I	TGATCA	1 cut(s)	256
KspAI	GTTAAC	1 cut(s)	2056
LmnI	GCTCC	3 cut(s)	882, 1664, 1990
Lsp1109I	GCAGC	3 cut(s)	1585, 1822, 1969
LweI	GCATC	4 cut(s)	1630, 1780, 1802, 1835
MabI	ACCWGGT	1 cut(s)	820
MaeI	CTAG	4 cut(s)	215, 665, 737, 1053
MaeII	ACGT	4 cut(s)	929, 1347, 1510, 2026
MaeIII	GTNAC	6 cut(s)	5, 284, 1456, 1628, 2104, 2137
MbiI	CCGCTC	1 cut(s)	571
MflI	RGATCY	5 cut(s)	334, 778, 1323, 1444, 1820
MhlI	GDGCHC	3 cut(s)	720, 879, 1763
MlyI	GAGTC	1 cut(s)	1777
MmeI	TCCRAC	5 cut(s)	331, 618, 935, 1108, 1471
Mph1103I	ATGCAT	1 cut(s)	1795
MroXI	GAANNNNTTC	2 cut(s)	1254, 1953
MseI	TTAA	6 cut(s)	219, 891, 965, 1478, 1485, 2055
MslI	CAYNNNNRTG	3 cut(s)	345, 665, 762
MspA1I	CMGCKG	1 cut(s)	1847
MspI	CCGG	2 cut(s)	1222, 1262
MspR9I	CCNGG	4 cut(s)	113, 795, 822, 1262
Mva1269I	GAATGC	2 cut(s)	773, 1810
MvaI	CCWGG	3 cut(s)	113, 795, 822
MwoI	GCNNNNNNNGC	6 cut(s)	1298, 1607, 1686, 1832, 1841, 1844
NciI	CCSGG	1 cut(s)	1262
NlaIV	GGNNCC	4 cut(s)	780, 1446, 1660, 1762
NmuCI	GTSAC	5 cut(s)	5, 284, 1628, 2104, 2137
NsiI	ATGCAT	1 cut(s)	1795
NspI	RCATGY	2 cut(s)	602, 875
NspV	TTCGAA	1 cut(s)	40
PagI	TCATGA	2 cut(s)	382, 513
PceI	AGGCCT	1 cut(s)	1525
PctI	GAATGC	2 cut(s)	773, 1810
PdmI	GAANNNNTTC	2 cut(s)	1254, 1953
PfeI	GAWTC	8 cut(s)	104, 232, 623, 857, 898, 919, 1244, 1883
PkrI	GCNGC	7 cut(s)	99, 573, 1600, 1682, 1837, 1846, 1984
PleI	GAGTC	1 cut(s)	1777
PpsI	GAGTC	1 cut(s)	1777
Psp6I	CCWGG	3 cut(s)	111, 793, 820
PspGI	CCWGG	3 cut(s)	111, 793, 820
PspN4I	GGNNCC	4 cut(s)	780, 1446, 1660, 1762
PspPI	GGNCC	2 cut(s)	1096, 1219
PstI	CTGCAG	1 cut(s)	2053
PstNI	CAGNNNCTG	1 cut(s)	1835
PsuI	RGATCY	5 cut(s)	334, 778, 1323, 1444, 1820
RsaI	GTAC	4 cut(s)	606, 950, 1591, 1768
RsaNI	GTAC	4 cut(s)	605, 949, 1590, 1767
RseI	CAYNNNNRTG	3 cut(s)	345, 665, 762
SaqAI	TTAA	6 cut(s)	219, 891, 965, 1478, 1485, 2055
SatI	GCNGC	7 cut(s)	98, 572, 1599, 1681, 1836, 1845, 1983
Sau96I	GGNCC	2 cut(s)	1096, 1219
SchI	GAGTC	1 cut(s)	1777
ScrFI	CCNGG	4 cut(s)	113, 795, 822, 1262
SduI	GDGCHC	3 cut(s)	720, 879, 1763
SexAI	ACCWGGT	1 cut(s)	820
SfaNI	GCATC	4 cut(s)	1630, 1780, 1802, 1835
SfcI	CTRYAG	1 cut(s)	2049
SfuI	TTCGAA	1 cut(s)	40
SinI	GGWCC	1 cut(s)	1096
SmiMI	CAYNNNNRTG	3 cut(s)	345, 665, 762
SmlI	CTYRAG	1 cut(s)	627
SmoI	CTYRAG	1 cut(s)	627
SpeI	ACTAGT	1 cut(s)	214
SseBI	AGGCCT	1 cut(s)	1525
SsiI	CCGC	7 cut(s)	97, 140, 571, 1071, 1681, 1845, 1862
SspI	AATATT	1 cut(s)	559
SspMI	CTAG	4 cut(s)	215, 665, 737, 1053
StuI	AGGCCT	1 cut(s)	1525
StyD4I	CCNGG	4 cut(s)	111, 793, 820, 1260
StyI	CCWWGG	3 cut(s)	1203, 1571, 1988
TaaI	ACNGT	3 cut(s)	499, 908, 1934
TaiI	ACGT	4 cut(s)	932, 1350, 1513, 2029
TaqI	TCGA	4 cut(s)	40, 420, 849, 1788
TatI	WGTACW	2 cut(s)	604, 948
TauI	GCSGC	4 cut(s)	100, 574, 1683, 1847
TfiI	GAWTC	8 cut(s)	104, 232, 623, 857, 898, 919, 1244, 1883
Tru1I	TTAA	6 cut(s)	219, 891, 965, 1478, 1485, 2055
Tru9I	TTAA	6 cut(s)	219, 891, 965, 1478, 1485, 2055
TscAI	CASTG	6 cut(s)	270, 502, 540, 849, 1567, 1633
TseFI	GTSAC	5 cut(s)	5, 284, 1628, 2104, 2137
TseI	GCWGC	3 cut(s)	1598, 1835, 1982
Tsp45I	GTSAC	5 cut(s)	5, 284, 1628, 2104, 2137
TspGWI	ACGGA	3 cut(s)	71, 227, 1987
TspRI	CASTG	6 cut(s)	270, 502, 540, 849, 1567, 1633
VpaK11BI	GGWCC	1 cut(s)	1096
XapI	RAATTY	2 cut(s)	298, 1950
XceI	RCATGY	2 cut(s)	602, 875
XmiI	GTMKAC	1 cut(s)	1697
XmnI	GAANNNNTTC	2 cut(s)	1254, 1953
XspI	CTAG	4 cut(s)	215, 665, 737, 1053
ZraI	GACGTC	1 cut(s)	930
Zsp2I	ATGCAT	1 cut(s)	1795

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.