Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0009541

Physical Location & Seq

Genomic Coordinates Reverse (-)

31464008 .. 31469786

Gene Structure 5779 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1229100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1566 bp

ATGGCAGTGAACGGACTGAAACTCGGTACGACGTCGCAGTCCTACTTAGAAGGCAAAGCAGTGAGCGACACCAAGGTTCTCATATCCGATCTCTGCCGCCAATTCTACAATCTCGGATGGGTCTCAGGAACGGGTGGCAGCATCACAATCAAAGTTCACGATGACTCTGTTCCGAAGCCTGAGCAGCTTGTAGTCATGTCTCCGTCTGGCGTTCAGAAGGAGAGAATGGTACCCGAGGACATGTATGTGTTATCTCCTAATGGGTCTGTCTTGTGTTCTCCATCTCCGAAGCCGTACCCGCATAAGCCTCCCAAGTGTTCTGATTGTGGTCCACTTTTTATGAAGGCATATGATATGCGTAATGCAGGAGCTGTTATTCATAGTCATGGGATGGAATCTTGTCTTGTAACAATGATCAATCCATTAGCAAAAGAATTTCGAATCACTCATATGGAGATGATTAAAGGAATCAAAGGGCACGGTTATTATGATGAACTTGTGGTCCCTATAATAGAGAACACTGCCTATGAATATGAGCTCACAGAGTCTCTTGCCAAAGCTATTGAAGCCTACCCAAAAACAACAGCTGTGCTTGTTCGTAACCATGGGATCTATGTCTGGGGTGACTCGTGGATCAATGCTAAGACTCAGGCTGAATGTTATCACTATCTCTTTGAAGCTGCTATCAAACTTCATCAGTTGGGTTTGGACCCAACTACTCCAAACCATGGCCCTATACACCAAAATGTCAAAGGAGTTTTAGGAAGTGGTATTCACGGAAATATATCTGTGAAGGCAGCGACTACAGCTTTAGATAATACATCTGAGCCGTCACAATGTTGCATTGTTCTTGACATTGAGGGAACTACTACTCCCATATCGTTTGTGACTGATGTTCTCTTTCCATATGCCCGCAATAATGTTGGAAGGCACTTGTCTGCAACATATGACACTGAAGAAACTCAAGATGACATAAAGTTGTTGCGCTTCCAGGTTCAAGAAGACCTGGAAAAAGGTGTTGCTGGTGCTGTTCCCATCCCCCCTGATGATGCAGGGAAAGAGGAGGTTGTTGCAGCTTTGGTTGCTAATGTTGACGCAATGATAAAAGCTGATCGGAAGATCACTGCCTTGAAACAGTTGCAAGGTCATATCTGGAGAACTGGATATGAAAAGAATGAATTGAAGGGACTAGTTTTCGATGATGTACCAGAAGCTCTTAAGAAGTGGCATGATTCAGGCATAAAGGTCTACATATACTCTAGTGGTAGCAGGTTGGCACAGAGACTTATATTTGGTAACTCAAATTATGGGGACCTAAGAGAATATTTGTCTGGATATTTTGACACCACAGTGGGAAACAAAAGAGAAAGTAGCAGTTATGCTGAAATTGTACAATCAGTTGGAGTTGATAAACCATCGGAGGTTTTATTTGTGACAGATGTCTTCCAAGAAGCTGTAGCTGCAAAGGCGGCAGGATTGGAAGTTATTGTATCGATCCGACCAGGGAATGGAGCTCTACCAGAGAATCATGGTTTCAAAACAATCACTTCTTTCTTGGAAATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00270 ko01100 map00270 map01100

Pfam Domains

Protein Families

Aldolase_II Hydrolase

Protein Analysis

522

Amino Acids

57.04

Weight (kDa)

5.69

Isoelectric Point (pI)

35.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aldolase_II	PF00596	28 - 228	2.9e-35	Class II Aldolase and Adducin N-terminal domain
Hydrolase	PF00702	281 - 492	4.8e-12	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009541)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53850 AT5G53850
fragaria_vesca	FvH4_3g19790 FvH4_3g19790
malus_domestica	MD03G1229100.v1.1 MD11G1249900.v1.1
prunus_persica	Prupe.4G180000_v2.0.a1 Prupe.4G180000_v2.0.a1 Prupe.4G180000_v2.0.a1 Prupe.4G180000_v2.0.a1 Prupe.4G180000_v2.0.a1 Prupe.4G180000_v2.0.a1
pyrus_communis	pycom03g17690
rosa_chinensis	RchiOBHm_Chr5g0033201
rosa_laevigata	RLG00000033457
rosa_multiflora	Rmu_ssc0000299.1_g000003
rosa_roxburghii	Rroxscaffold_1G00046780
rosa_rugosa	Rorug05G0137700 Rorug05G0137800
rosa_samantha	Rh5BG230400 Rh5CG258300
rosa_wichuraiana	Rw5G020980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	37
AatII	GACGTC	1 cut(s)	35
Acc36I	ACCTGC	1 cut(s)	1260
Acc65I	GGTACC	1 cut(s)	229
AccB1I	GGYRCC	1 cut(s)	229
AccB7I	CCANNNNNTGG	2 cut(s)	728, 1508
AccI	GTMKAC	1 cut(s)	1248
AciI	CCGC	4 cut(s)	97, 299, 913, 1469
AclWI	GGATC	3 cut(s)	617, 641, 1489
AcsI	RAATTY	1 cut(s)	434
AcuI	CTGAAG	1 cut(s)	975
AcyI	GRCGYC	1 cut(s)	32
AfaI	GTAC	5 cut(s)	28, 231, 296, 1206, 1392
AfiI	CCNNNNNNNGG	2 cut(s)	728, 1508
AflII	CTTAAG	1 cut(s)	1217
AflIII	ACRYGT	1 cut(s)	240
AgsI	TTSAA	6 cut(s)	566, 677, 998, 1132, 1183, 1537
AhlI	ACTAGT	1 cut(s)	1189
AjnI	CCWGG	3 cut(s)	990, 1005, 1501
AleI	CACNNNNGTG	1 cut(s)	1349
AloI	GAACNNNNNNTCC	4 cut(s)	486, 518, 856, 888
Alw21I	GWGCWC	2 cut(s)	540, 1516
Alw26I	GTCTC	4 cut(s)	127, 204, 552, 1276
AlwI	GGATC	3 cut(s)	617, 641, 1489
AlwNI	CAGNNNCTG	1 cut(s)	371
Ama87I	CYCGRG	1 cut(s)	233
AoxI	GGCC	1 cut(s)	730
ApeKI	GCWGC	6 cut(s)	138, 184, 680, 797, 1073, 1460
ApoI	RAATTY	1 cut(s)	434
Asp718I	GGTACC	1 cut(s)	229
AspLEI	GCGC	1 cut(s)	987
AspS9I	GGNCC	5 cut(s)	329, 502, 709, 731, 1312
AsuHPI	GGTGA	1 cut(s)	635
AsuII	TTCGAA	1 cut(s)	439
AvaI	CYCGRG	1 cut(s)	233
AvaII	GGWCC	4 cut(s)	329, 502, 709, 1312
BaeGI	GKGCMC	1 cut(s)	480
BanI	GGYRCC	1 cut(s)	229
BanII	GRGCYC	2 cut(s)	540, 1516
BauI	CACGAG	1 cut(s)	628
BbsI	GAAGAC	2 cut(s)	1008, 1435
Bbv12I	GWGCWC	2 cut(s)	540, 1516
BbvI	GCAGC	6 cut(s)	150, 196, 667, 809, 1085, 1447
BccI	CCATC	5 cut(s)	111, 289, 385, 1043, 1423
BceAI	ACGGC	2 cut(s)	277, 814
BciT130I	CCWGG	3 cut(s)	992, 1007, 1503
BclI	TGATCA	1 cut(s)	414
BcoDI	GTCTC	4 cut(s)	127, 204, 552, 1276
BcuI	ACTAGT	1 cut(s)	1189
BfaI	CTAG	2 cut(s)	1190, 1260
BfmI	CTRYAG	2 cut(s)	804, 1455
BfrI	CTTAAG	1 cut(s)	1217
BfuAI	ACCTGC	1 cut(s)	1260
BisI	GCNGC	8 cut(s)	97, 139, 185, 681, 798, 1074, 1461, 1470
BlsI	GCNGC	8 cut(s)	98, 140, 186, 682, 799, 1075, 1462, 1471
Bme1390I	CCNGG	3 cut(s)	992, 1007, 1503
Bme18I	GGWCC	4 cut(s)	329, 502, 709, 1312
BmeT110I	CYCGRG	1 cut(s)	233
BmgT120I	GGNCC	5 cut(s)	329, 502, 709, 731, 1312
BmiI	GGNNCC	4 cut(s)	231, 504, 711, 1313
BmrFI	CCNGG	3 cut(s)	992, 1007, 1503
BmsI	GCATC	2 cut(s)	150, 1039
BoxI	GACNNNNGTC	1 cut(s)	1439
BpiI	GAAGAC	2 cut(s)	1008, 1435
BpmI	CTGGAG	1 cut(s)	1174
Bpu10I	CCTNAGC	1 cut(s)	180
Bpu14I	TTCGAA	1 cut(s)	439
BpuEI	CTTGAG	1 cut(s)	948
Bsa29I	ATCGAT	1 cut(s)	1493
BsaHI	GRCGYC	1 cut(s)	32
BsaI	GGTCTC	1 cut(s)	127
BsaJI	CCNNGG	5 cut(s)	72, 234, 604, 727, 1502
BsaXI	ACNNNNNCTCC	2 cut(s)	856, 886
Bsc4I	CCNNNNNNNGG	2 cut(s)	728, 1508
Bse1I	ACTGG	1 cut(s)	1165
Bse3DI	GCAATG	1 cut(s)	1104
BseBI	CCWGG	3 cut(s)	992, 1007, 1503
BseCI	ATCGAT	1 cut(s)	1493
BseDI	CCNNGG	5 cut(s)	72, 234, 604, 727, 1502
BseGI	GGATG	3 cut(s)	122, 396, 1035
BseLI	CCNNNNNNNGG	2 cut(s)	728, 1508
BseMI	GCAATG	1 cut(s)	1104
BseMII	CTCAG	4 cut(s)	138, 171, 662, 816
BseNI	ACTGG	1 cut(s)	1165
BseRI	GAGGAG	1 cut(s)	1076
BseSI	GKGCMC	1 cut(s)	480
BseXI	GCAGC	6 cut(s)	150, 196, 667, 809, 1085, 1447
BshFI	GGCC	1 cut(s)	732
BshNI	GGYRCC	1 cut(s)	229
BshVI	ATCGAT	1 cut(s)	1493
BsiHKAI	GWGCWC	2 cut(s)	540, 1516
BsiHKCI	CYCGRG	1 cut(s)	233
BslFI	GGGAC	3 cut(s)	488, 1200, 1325
BslI	CCNNNNNNNGG	2 cut(s)	728, 1508
BsmAI	GTCTC	4 cut(s)	127, 204, 552, 1276
BsmFI	GGGAC	3 cut(s)	488, 1200, 1325
BsnI	GGCC	1 cut(s)	732
Bso31I	GGTCTC	1 cut(s)	127
BsoBI	CYCGRG	1 cut(s)	233
Bsp119I	TTCGAA	1 cut(s)	439
Bsp1286I	GDGCHC	3 cut(s)	480, 540, 1516
Bsp1407I	TGTACA	1 cut(s)	1390
Bsp143I	GATC	7 cut(s)	88, 414, 609, 633, 1111, 1119, 1494
Bsp19I	CCATGG	2 cut(s)	604, 727
BspACI	CCGC	4 cut(s)	97, 299, 913, 1469
BspANI	GGCC	1 cut(s)	732
BspCNI	CTCAG	4 cut(s)	137, 172, 661, 817
BspDI	ATCGAT	1 cut(s)	1493
BspLI	GGNNCC	4 cut(s)	231, 504, 711, 1313
BspMI	ACCTGC	1 cut(s)	1260
BspPI	GGATC	3 cut(s)	617, 641, 1489
BspT104I	TTCGAA	1 cut(s)	439
BspT107I	GGYRCC	1 cut(s)	229
BspTI	CTTAAG	1 cut(s)	1217
BspTNI	GGTCTC	1 cut(s)	127
BsrDI	GCAATG	1 cut(s)	1104
BsrGI	TGTACA	1 cut(s)	1390
BsrI	ACTGG	1 cut(s)	1165
BssECI	CCNNGG	5 cut(s)	72, 234, 604, 727, 1502
BssMI	GATC	7 cut(s)	88, 414, 609, 633, 1111, 1119, 1494
BssNI	GRCGYC	1 cut(s)	32
BssSI	CACGAG	1 cut(s)	628
BssT1I	CCWWGG	3 cut(s)	72, 604, 727
Bst2BI	CACGAG	1 cut(s)	628
Bst2UI	CCWGG	3 cut(s)	992, 1007, 1503
Bst4CI	ACNGT	3 cut(s)	482, 1137, 1351
BstACI	GRCGYC	1 cut(s)	32
BstAFI	CTTAAG	1 cut(s)	1217
BstAUI	TGTACA	1 cut(s)	1390
BstBI	TTCGAA	1 cut(s)	439
BstC8I	GCNNGC	1 cut(s)	913
BstDEI	CTNAG	7 cut(s)	46, 124, 180, 642, 648, 825, 1316
BstDSI	CCRYGG	2 cut(s)	604, 727
BstF5I	GGATG	3 cut(s)	122, 396, 1035
BstHHI	GCGC	1 cut(s)	987
BstKTI	GATC	7 cut(s)	91, 417, 612, 636, 1114, 1122, 1497
BstMAI	GTCTC	4 cut(s)	127, 204, 552, 1276
BstMBI	GATC	7 cut(s)	88, 414, 609, 633, 1111, 1119, 1494
BstMWI	GCNNNNNNNGC	8 cut(s)	184, 298, 566, 806, 1082, 1460, 1466, 1469
BstNI	CCWGG	3 cut(s)	992, 1007, 1503
BstNSI	RCATGY	1 cut(s)	244
BstPAI	GACNNNNGTC	1 cut(s)	1439
BstSCI	CCNGG	3 cut(s)	990, 1005, 1501
BstSFI	CTRYAG	2 cut(s)	804, 1455
BstSLI	GKGCMC	1 cut(s)	480
BstV1I	GCAGC	6 cut(s)	150, 196, 667, 809, 1085, 1447
BstV2I	GAAGAC	2 cut(s)	1008, 1435
BstX2I	RGATCY	1 cut(s)	609
BstYI	RGATCY	1 cut(s)	609
Bsu15I	ATCGAT	1 cut(s)	1493
BsuRI	GGCC	1 cut(s)	732
BsuTUI	ATCGAT	1 cut(s)	1493
BtgI	CCRYGG	2 cut(s)	604, 727
BtsCI	GGATG	3 cut(s)	122, 396, 1035
BtsI	GCAGTG	4 cut(s)	12, 66, 519, 1122
BtsIMutI	CAGTG	6 cut(s)	12, 66, 519, 951, 1122, 1356
BveI	ACCTGC	1 cut(s)	1260
Cac8I	GCNNGC	1 cut(s)	913
CaiI	CAGNNNCTG	1 cut(s)	371
CfoI	GCGC	1 cut(s)	987
Cfr13I	GGNCC	5 cut(s)	329, 502, 709, 731, 1312
ClaI	ATCGAT	1 cut(s)	1493
CseI	GACGC	1 cut(s)	1103
Csp6I	GTAC	5 cut(s)	27, 230, 295, 1205, 1391
CviAII	CATG	7 cut(s)	196, 241, 386, 605, 728, 1229, 1529
CviQI	GTAC	5 cut(s)	27, 230, 295, 1205, 1391
DdeI	CTNAG	7 cut(s)	46, 124, 180, 642, 648, 825, 1316
DpnI	GATC	7 cut(s)	90, 416, 611, 635, 1113, 1121, 1496
DpnII	GATC	7 cut(s)	88, 414, 609, 633, 1111, 1119, 1494
DrdI	GACNNNNNNGTC	1 cut(s)	37
DseDI	GACNNNNNNGTC	1 cut(s)	37
Ecl136II	GAGCTC	2 cut(s)	538, 1514
Eco130I	CCWWGG	3 cut(s)	72, 604, 727
Eco24I	GRGCYC	2 cut(s)	540, 1516
Eco31I	GGTCTC	1 cut(s)	127
Eco47I	GGWCC	4 cut(s)	329, 502, 709, 1312
Eco53kI	GAGCTC	2 cut(s)	538, 1514
Eco57I	CTGAAG	1 cut(s)	975
Eco88I	CYCGRG	1 cut(s)	233
EcoICRI	GAGCTC	2 cut(s)	538, 1514
EcoO109I	RGGNCCY	1 cut(s)	1312
EcoRII	CCWGG	3 cut(s)	990, 1005, 1501
EcoT14I	CCWWGG	3 cut(s)	72, 604, 727
EcoT38I	GRGCYC	2 cut(s)	540, 1516
ErhI	CCWWGG	3 cut(s)	72, 604, 727
FaeI	CATG	7 cut(s)	199, 244, 389, 608, 731, 1232, 1532
FaqI	GGGAC	3 cut(s)	488, 1200, 1325
FatI	CATG	7 cut(s)	195, 240, 385, 604, 727, 1228, 1528
FauI	CCCGC	2 cut(s)	306, 920
FauNDI	CATATG	4 cut(s)	349, 450, 907, 946
FbaI	TGATCA	1 cut(s)	414
FblI	GTMKAC	1 cut(s)	1248
Fnu4HI	GCNGC	8 cut(s)	97, 139, 185, 681, 798, 1074, 1461, 1470
FokI	GGATG	3 cut(s)	129, 403, 1022
FriOI	GRGCYC	2 cut(s)	540, 1516
Fsp4HI	GCNGC	8 cut(s)	97, 139, 185, 681, 798, 1074, 1461, 1470
FspBI	CTAG	2 cut(s)	1190, 1260
GlaI	GCGC	1 cut(s)	986
GluI	GCNGC	8 cut(s)	97, 139, 185, 681, 798, 1074, 1461, 1470
GsuI	CTGGAG	1 cut(s)	1174
HaeIII	GGCC	1 cut(s)	732
HgaI	GACGC	1 cut(s)	1103
HhaI	GCGC	1 cut(s)	987
Hin1I	GRCGYC	1 cut(s)	32
Hin1II	CATG	7 cut(s)	199, 244, 389, 608, 731, 1232, 1532
Hin6I	GCGC	1 cut(s)	985
HinP1I	GCGC	1 cut(s)	985
HincII	GTYRAC	1 cut(s)	1093
HindII	GTYRAC	1 cut(s)	1093
HinfI	GANTC	9 cut(s)	164, 395, 441, 468, 545, 626, 646, 1232, 1525
HphI	GGTGA	1 cut(s)	635
Hpy166II	GTNNAC	5 cut(s)	10, 157, 332, 1093, 1249
Hpy188III	TCNNGA	7 cut(s)	126, 158, 851, 965, 998, 1153, 1332
Hpy8I	GTNNAC	5 cut(s)	10, 157, 332, 1093, 1249
Hpy99I	CGWCG	2 cut(s)	34, 37
HpyAV	CCTTC	6 cut(s)	44, 211, 337, 787, 921, 1177
HpyCH4III	ACNGT	3 cut(s)	482, 1137, 1351
HpyCH4IV	ACGT	1 cut(s)	32
HpyCH4V	TGCA	7 cut(s)	365, 843, 941, 1052, 1073, 1141, 1463
HpyF10VI	GCNNNNNNNGC	8 cut(s)	184, 298, 566, 806, 1082, 1460, 1466, 1469
HpyF3I	CTNAG	7 cut(s)	46, 124, 180, 642, 648, 825, 1316
HpySE526I	ACGT	1 cut(s)	32
Hsp92I	GRCGYC	1 cut(s)	32
Hsp92II	CATG	7 cut(s)	199, 244, 389, 608, 731, 1232, 1532
HspAI	GCGC	1 cut(s)	985
KpnI	GGTACC	1 cut(s)	233
Ksp22I	TGATCA	1 cut(s)	414
Kzo9I	GATC	7 cut(s)	88, 414, 609, 633, 1111, 1119, 1494
LmnI	GCTCC	2 cut(s)	368, 1511
Lsp1109I	GCAGC	6 cut(s)	150, 196, 667, 809, 1085, 1447
LweI	GCATC	2 cut(s)	150, 1039
MaeI	CTAG	2 cut(s)	1190, 1260
MaeII	ACGT	1 cut(s)	32
MaeIII	GTNAC	7 cut(s)	406, 599, 623, 831, 886, 1295, 1432
MalI	GATC	7 cut(s)	90, 416, 611, 635, 1113, 1121, 1496
MboI	GATC	7 cut(s)	88, 414, 609, 633, 1111, 1119, 1494
MboII	GAAGA	4 cut(s)	968, 1013, 1129, 1435
MflI	RGATCY	1 cut(s)	609
MhlI	GDGCHC	3 cut(s)	480, 540, 1516
MluCI	AATT	5 cut(s)	101, 434, 1178, 1303, 1386
MlyI	GAGTC	4 cut(s)	158, 554, 620, 640
MmeI	TCCRAC	3 cut(s)	904, 1381, 1522
MnlI	CCTC	6 cut(s)	229, 318, 853, 1054, 1057, 1414
MseI	TTAA	2 cut(s)	462, 1218
MslI	CAYNNNNRTG	5 cut(s)	245, 384, 449, 744, 1349
MspA1I	CMGCKG	1 cut(s)	587
MspCI	CTTAAG	1 cut(s)	1217
MspR9I	CCNGG	3 cut(s)	992, 1007, 1503
MvaI	CCWGG	3 cut(s)	992, 1007, 1503
MwoI	GCNNNNNNNGC	8 cut(s)	184, 298, 566, 806, 1082, 1460, 1466, 1469
NcoI	CCATGG	2 cut(s)	604, 727
NdeI	CATATG	4 cut(s)	349, 450, 907, 946
NdeII	GATC	7 cut(s)	88, 414, 609, 633, 1111, 1119, 1494
NlaIII	CATG	7 cut(s)	199, 244, 389, 608, 731, 1232, 1532
NlaIV	GGNNCC	4 cut(s)	231, 504, 711, 1313
NmuCI	GTSAC	4 cut(s)	623, 831, 886, 1432
NspI	RCATGY	1 cut(s)	244
NspV	TTCGAA	1 cut(s)	439
OliI	CACNNNNGTG	1 cut(s)	1349
PciI	ACATGT	1 cut(s)	240
PfeI	GAWTC	5 cut(s)	395, 441, 468, 1232, 1525
PflMI	CCANNNNNTGG	2 cut(s)	728, 1508
PkrI	GCNGC	8 cut(s)	98, 140, 186, 682, 799, 1075, 1462, 1471
PleI	GAGTC	4 cut(s)	158, 553, 620, 640
PpsI	GAGTC	4 cut(s)	158, 553, 620, 640
PpuMI	RGGWCCY	1 cut(s)	1312
PscI	ACATGT	1 cut(s)	240
PshAI	GACNNNNGTC	1 cut(s)	1439
Psp124BI	GAGCTC	2 cut(s)	540, 1516
Psp5II	RGGWCCY	1 cut(s)	1312
Psp6I	CCWGG	3 cut(s)	990, 1005, 1501
PspGI	CCWGG	3 cut(s)	990, 1005, 1501
PspN4I	GGNNCC	4 cut(s)	231, 504, 711, 1313
PspPI	GGNCC	5 cut(s)	329, 502, 709, 731, 1312
PspPPI	RGGWCCY	1 cut(s)	1312
PstNI	CAGNNNCTG	1 cut(s)	371
PsuI	RGATCY	1 cut(s)	609
PvuII	CAGCTG	1 cut(s)	587
RsaI	GTAC	5 cut(s)	28, 231, 296, 1206, 1392
RsaNI	GTAC	5 cut(s)	27, 230, 295, 1205, 1391
RseI	CAYNNNNRTG	5 cut(s)	245, 384, 449, 744, 1349
SacI	GAGCTC	2 cut(s)	540, 1516
SaqAI	TTAA	2 cut(s)	462, 1218
SatI	GCNGC	8 cut(s)	97, 139, 185, 681, 798, 1074, 1461, 1470
Sau3AI	GATC	7 cut(s)	88, 414, 609, 633, 1111, 1119, 1494
Sau96I	GGNCC	5 cut(s)	329, 502, 709, 731, 1312
SchI	GAGTC	4 cut(s)	158, 554, 620, 640
ScrFI	CCNGG	3 cut(s)	992, 1007, 1503
SduI	GDGCHC	3 cut(s)	480, 540, 1516
SfaNI	GCATC	2 cut(s)	150, 1039
SfcI	CTRYAG	2 cut(s)	804, 1455
SfuI	TTCGAA	1 cut(s)	439
SinI	GGWCC	4 cut(s)	329, 502, 709, 1312
SmiMI	CAYNNNNRTG	5 cut(s)	245, 384, 449, 744, 1349
SmlI	CTYRAG	2 cut(s)	963, 1217
SmoI	CTYRAG	2 cut(s)	963, 1217
SpeI	ACTAGT	1 cut(s)	1189
Sse9I	AATT	5 cut(s)	101, 434, 1178, 1303, 1386
SsiI	CCGC	4 cut(s)	97, 299, 913, 1469
SspI	AATATT	1 cut(s)	1325
SspMI	CTAG	2 cut(s)	1190, 1260
SstI	GAGCTC	2 cut(s)	540, 1516
StyD4I	CCNGG	3 cut(s)	990, 1005, 1501
StyI	CCWWGG	3 cut(s)	72, 604, 727
TaaI	ACNGT	3 cut(s)	482, 1137, 1351
TaiI	ACGT	1 cut(s)	35
TaqI	TCGA	3 cut(s)	439, 1197, 1493
TasI	AATT	5 cut(s)	101, 434, 1178, 1303, 1386
TatI	WGTACW	1 cut(s)	1390
TauI	GCSGC	2 cut(s)	99, 1472
TfiI	GAWTC	5 cut(s)	395, 441, 468, 1232, 1525
Tru1I	TTAA	2 cut(s)	462, 1218
Tru9I	TTAA	2 cut(s)	462, 1218
TscAI	CASTG	6 cut(s)	12, 66, 526, 958, 1129, 1356
TseFI	GTSAC	4 cut(s)	623, 831, 886, 1432
TseI	GCWGC	6 cut(s)	138, 184, 680, 797, 1073, 1460
Tsp45I	GTSAC	4 cut(s)	623, 831, 886, 1432
TspDTI	ATGAA	7 cut(s)	356, 368, 507, 543, 683, 1182, 1191
TspGWI	ACGGA	3 cut(s)	27, 192, 792
TspRI	CASTG	6 cut(s)	12, 66, 526, 958, 1129, 1356
Van91I	CCANNNNNTGG	2 cut(s)	728, 1508
Vha464I	CTTAAG	1 cut(s)	1217
VpaK11BI	GGWCC	4 cut(s)	329, 502, 709, 1312
XapI	RAATTY	1 cut(s)	434
XceI	RCATGY	1 cut(s)	244
XmiI	GTMKAC	1 cut(s)	1248
XspI	CTAG	2 cut(s)	1190, 1260
ZraI	GACGTC	1 cut(s)	33

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.