Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0009541

Physical Location & Seq

Genomic Coordinates Reverse (-)

36208684 .. 36215185

Gene Structure 6502 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1249900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1635 bp

ATGGCATCAGTGGCAGTGAACGGACTGAAACTCGGTACGACGTCGCAGGCCTACTTGGAAGGCAAAGCGGTGAGCGACACCAAGGTTCTGATATCCGACCTCTGCCGCCAGTTCTACAATCTCGGATGGGTCTCAGGGACCGGCGGCAGCATCACAATCAAAGTGCACGACGACTCTATTCCTAAGCCCGAGCAGCTCGTAGTCATGTCTCCATCTGGCGTTCAGAAGGAGAGAATGGTACCAGAGGACATGTATGTGTTATCTCCGAATGGGTCTGTCTTGTGTACTCCATCTCCTAAACCCTACCCACATAAGCCTCCCAAGTGTTCTGATTGTGGTCCACTTTTTATGAAGAATGTAGCCAAGAGGCGATTGATTTTGCGAAGAGAAATGTCAAGGAATCTCAGTTACAAGGCATATGAGATGCGTAATGCTGGAGCTGTTATTCATAGTCATGGGATGGAATCTTGTCTTGTAACAATGATCAATCCTAATGCAAAAGAATTTCGAATCACCCATATGGAGATGATCAAAGGAATCAAAGGGCACGGTTATTATGATGAGCTTGTGGTCCCTATAATAGAGAATACTGCCTATGAATATGAGCTCACAGAGTCTCTTGCCAAAGCTATTGAAGCCTACCCGAAAACAACAGCCGTTCTTGTTCGTAACCATGGGATCTATATATGGGGAGACTCGTGGATCAATGCTAAGACTCAGGCTGAATGTTATCACTATCTCTTTGAAGCTGCTATCAAACTTCATCAGTTGGGTTTGGACCCAACTACTCCAAACCATGGTCCTATAAAACAAAATGTCAAGGGAGTTTCGGGAAGTGGTATTTGCAGAAACATATCTGTGAACGCTGGGACTGCAGCTTCAGATAATAAATTTGAGCCATCACGACGTTGCATTGTTCTTGACATCGAGGGGACTACTACTCCCATATCGTTTGTGACTGATGTTCTCTTTCCATATGCCCGCGATAATGTTGGAAGGCACTTGTCTGCAACATATGACACTGAAGAAACTCAAGATGACATAAAGTTGTTGCGCTTCCAGGTTCAAGAAGACCTGGAAAAAGGTGTTGCTGGTGCTGTTCCCATCCCCTCTGATGATGCAGGGAAAGAGGAGGTTGTTCAAGCTTTGGTTGCTAATGTTGACTCGATGATAAAAGCTGATCGGAAGATCACTGCCTTGAAACAGTTGCAAGGTCATATCTGGAGAACTGGATTTGAAAAGAATGAATTGACAGGAGTAGTTTTCGATGATGTACCAGAAGCTCTTAAGAAATGGCATGATTCAGGCATAAAGGTGTACATATACTCCAGTGGTAGCAGGTTGGCACAGAGACTTATATTTGGTAACTCAAATTATGGGGACCTAAGAGAATATTTGTCTGGATTTTTTGACACCACAGTGGGAAACAAAAGAGAAAGTAGCAGTTATGCTGAAATTGTACAATCAGTTGGAGTTGATAAACCATCGGAGGTTTTATTTGTGACGGATGTCTTTCAAGAAGCTGTAGCTGCAAAGGCAGCAGGATTGGGAGTTGCTGTATCGATCCGACCAGGGAATGGAGCTCTTCCAGAGAATCATGGTTTCATGACAATCACTTCTTTCCTGGAAATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00270 ko01100 map00270 map01100

Pfam Domains

Protein Families

Aldolase_II Hydrolase

Protein Analysis

545

Amino Acids

59.82

Weight (kDa)

6.3

Isoelectric Point (pI)

39.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aldolase_II	PF00596	31 - 251	4.7e-29	Class II Aldolase and Adducin N-terminal domain
Hydrolase	PF00702	303 - 515	5.6e-12	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009541)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53850 AT5G53850
fragaria_vesca	FvH4_3g19790 FvH4_3g19790
malus_domestica	MD03G1229100.v1.1 MD11G1249900.v1.1
prunus_persica	Prupe.4G180000_v2.0.a1 Prupe.4G180000_v2.0.a1 Prupe.4G180000_v2.0.a1 Prupe.4G180000_v2.0.a1 Prupe.4G180000_v2.0.a1 Prupe.4G180000_v2.0.a1
pyrus_communis	pycom03g17690
rosa_chinensis	RchiOBHm_Chr5g0033201
rosa_laevigata	RLG00000033457
rosa_multiflora	Rmu_ssc0000299.1_g000003
rosa_roxburghii	Rroxscaffold_1G00046780
rosa_rugosa	Rorug05G0137700 Rorug05G0137800
rosa_samantha	Rh5BG230400 Rh5CG258300
rosa_wichuraiana	Rw5G020980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	44
Acc36I	ACCTGC	1 cut(s)	1329
Acc65I	GGTACC	1 cut(s)	238
AccB1I	GGYRCC	1 cut(s)	238
AccB7I	CCANNNNNTGG	2 cut(s)	797, 1577
AccII	CGCG	1 cut(s)	984
AciI	CCGC	4 cut(s)	68, 106, 144, 982
AclWI	GGATC	3 cut(s)	686, 710, 1558
AcsI	RAATTY	2 cut(s)	503, 890
AcuI	CTGAAG	2 cut(s)	864, 1044
AcyI	GRCGYC	1 cut(s)	41
AfaI	GTAC	6 cut(s)	37, 240, 286, 1275, 1319, 1461
AfiI	CCNNNNNNNGG	2 cut(s)	797, 1577
AflII	CTTAAG	1 cut(s)	1286
AflIII	ACRYGT	1 cut(s)	249
AgsI	TTSAA	7 cut(s)	635, 746, 1067, 1142, 1201, 1238, 1517
AjnI	CCWGG	4 cut(s)	1059, 1074, 1570, 1623
AleI	CACNNNNGTG	1 cut(s)	1418
Alw21I	GWGCWC	3 cut(s)	168, 609, 1585
Alw26I	GTCTC	5 cut(s)	136, 213, 621, 687, 1345
Alw44I	GTGCAC	1 cut(s)	164
AlwI	GGATC	3 cut(s)	686, 710, 1558
Ama87I	CYCGRG	1 cut(s)	188
AoxI	GGCC	1 cut(s)	48
ApaLI	GTGCAC	1 cut(s)	164
ApeKI	GCWGC	6 cut(s)	147, 193, 749, 875, 1529, 1538
ApoI	RAATTY	2 cut(s)	503, 890
Asp718I	GGTACC	1 cut(s)	238
AspLEI	GCGC	1 cut(s)	1056
AspS9I	GGNCC	6 cut(s)	138, 338, 571, 778, 800, 1381
AsuHPI	GGTGA	2 cut(s)	82, 505
AsuII	TTCGAA	1 cut(s)	508
AvaI	CYCGRG	1 cut(s)	188
AvaII	GGWCC	6 cut(s)	138, 338, 571, 778, 800, 1381
BaeGI	GKGCMC	2 cut(s)	168, 549
BanI	GGYRCC	1 cut(s)	238
BanII	GRGCYC	2 cut(s)	609, 1585
BauI	CACGAG	1 cut(s)	697
BbsI	GAAGAC	1 cut(s)	1077
Bbv12I	GWGCWC	3 cut(s)	168, 609, 1585
BbvI	GCAGC	6 cut(s)	159, 205, 736, 887, 1516, 1550
BccI	CCATC	7 cut(s)	120, 220, 298, 454, 907, 1112, 1492
BceAI	ACGGC	1 cut(s)	641
BciT130I	CCWGG	4 cut(s)	1061, 1076, 1572, 1625
BclI	TGATCA	2 cut(s)	483, 528
BcoDI	GTCTC	5 cut(s)	136, 213, 621, 687, 1345
BfmI	CTRYAG	2 cut(s)	873, 1524
BfrI	CTTAAG	1 cut(s)	1286
BfuAI	ACCTGC	1 cut(s)	1329
BisI	GCNGC	8 cut(s)	106, 145, 148, 194, 750, 876, 1530, 1539
BlsI	GCNGC	8 cut(s)	107, 146, 149, 195, 751, 877, 1531, 1540
Bme1390I	CCNGG	4 cut(s)	1061, 1076, 1572, 1625
Bme18I	GGWCC	6 cut(s)	138, 338, 571, 778, 800, 1381
BmeT110I	CYCGRG	1 cut(s)	188
BmgT120I	GGNCC	6 cut(s)	138, 338, 571, 778, 800, 1381
BmiI	GGNNCC	5 cut(s)	139, 240, 573, 780, 1382
BmrFI	CCNGG	4 cut(s)	1061, 1076, 1572, 1625
BmsI	GCATC	4 cut(s)	14, 159, 414, 1108
BoxI	GACNNNNGTC	1 cut(s)	1508
BpiI	GAAGAC	1 cut(s)	1077
BpmI	CTGGAG	3 cut(s)	456, 1243, 1312
Bpu10I	CCTNAGC	1 cut(s)	183
Bpu14I	TTCGAA	1 cut(s)	508
BpuEI	CTTGAG	1 cut(s)	1017
Bsa29I	ATCGAT	1 cut(s)	1562
BsaHI	GRCGYC	1 cut(s)	41
BsaI	GGTCTC	1 cut(s)	136
BsaJI	CCNNGG	4 cut(s)	81, 673, 796, 1571
BsaXI	ACNNNNNCTCC	8 cut(s)	277, 307, 925, 955, 1310, 1340, 1542, 1572
Bsc4I	CCNNNNNNNGG	2 cut(s)	797, 1577
Bse118I	RCCGGY	1 cut(s)	140
Bse1I	ACTGG	3 cut(s)	109, 1234, 1329
BseBI	CCWGG	4 cut(s)	1061, 1076, 1572, 1625
BseCI	ATCGAT	1 cut(s)	1562
BseDI	CCNNGG	4 cut(s)	81, 673, 796, 1571
BseGI	GGATG	4 cut(s)	131, 465, 1104, 1513
BseLI	CCNNNNNNNGG	2 cut(s)	797, 1577
BseMII	CTCAG	3 cut(s)	147, 418, 731
BseNI	ACTGG	3 cut(s)	109, 1234, 1329
BseRI	GAGGAG	1 cut(s)	1145
BseSI	GKGCMC	2 cut(s)	168, 549
BseXI	GCAGC	6 cut(s)	159, 205, 736, 887, 1516, 1550
BseYI	CCCAGC	1 cut(s)	866
Bsh1236I	CGCG	1 cut(s)	984
BshFI	GGCC	1 cut(s)	50
BshNI	GGYRCC	1 cut(s)	238
BshVI	ATCGAT	1 cut(s)	1562
BsiHKAI	GWGCWC	3 cut(s)	168, 609, 1585
BsiHKCI	CYCGRG	1 cut(s)	188
BsiSI	CCGG	1 cut(s)	141
BslFI	GGGAC	5 cut(s)	151, 557, 883, 946, 1394
BslI	CCNNNNNNNGG	2 cut(s)	797, 1577
BsmAI	GTCTC	5 cut(s)	136, 213, 621, 687, 1345
BsmFI	GGGAC	5 cut(s)	151, 557, 883, 946, 1394
BsnI	GGCC	1 cut(s)	50
Bso31I	GGTCTC	1 cut(s)	136
BsoBI	CYCGRG	1 cut(s)	188
Bsp119I	TTCGAA	1 cut(s)	508
Bsp1286I	GDGCHC	4 cut(s)	168, 549, 609, 1585
Bsp1407I	TGTACA	2 cut(s)	1317, 1459
Bsp143I	GATC	7 cut(s)	483, 528, 678, 702, 1180, 1188, 1563
Bsp19I	CCATGG	2 cut(s)	673, 796
BspACI	CCGC	4 cut(s)	68, 106, 144, 982
BspANI	GGCC	1 cut(s)	50
BspCNI	CTCAG	3 cut(s)	146, 417, 730
BspDI	ATCGAT	1 cut(s)	1562
BspFNI	CGCG	1 cut(s)	984
BspHI	TCATGA	1 cut(s)	1605
BspLI	GGNNCC	5 cut(s)	139, 240, 573, 780, 1382
BspMAI	CTGCAG	1 cut(s)	877
BspMI	ACCTGC	1 cut(s)	1329
BspPI	GGATC	3 cut(s)	686, 710, 1558
BspQI	GCTCTTC	1 cut(s)	1590
BspT104I	TTCGAA	1 cut(s)	508
BspT107I	GGYRCC	1 cut(s)	238
BspTI	CTTAAG	1 cut(s)	1286
BspTNI	GGTCTC	1 cut(s)	136
BsrFI	RCCGGY	1 cut(s)	140
BsrGI	TGTACA	2 cut(s)	1317, 1459
BsrI	ACTGG	3 cut(s)	109, 1234, 1329
BssAI	RCCGGY	1 cut(s)	140
BssECI	CCNNGG	4 cut(s)	81, 673, 796, 1571
BssMI	GATC	7 cut(s)	483, 528, 678, 702, 1180, 1188, 1563
BssNI	GRCGYC	1 cut(s)	41
BssSI	CACGAG	1 cut(s)	697
BssT1I	CCWWGG	3 cut(s)	81, 673, 796
Bst2BI	CACGAG	1 cut(s)	697
Bst2UI	CCWGG	4 cut(s)	1061, 1076, 1572, 1625
Bst4CI	ACNGT	3 cut(s)	551, 1206, 1420
Bst6I	CTCTTC	2 cut(s)	379, 1590
BstACI	GRCGYC	1 cut(s)	41
BstAFI	CTTAAG	1 cut(s)	1286
BstAUI	TGTACA	2 cut(s)	1317, 1459
BstBI	TTCGAA	1 cut(s)	508
BstC8I	GCNNGC	2 cut(s)	48, 982
BstDEI	CTNAG	6 cut(s)	133, 183, 404, 711, 717, 1385
BstDSI	CCRYGG	2 cut(s)	673, 796
BstF5I	GGATG	4 cut(s)	131, 465, 1104, 1513
BstFNI	CGCG	1 cut(s)	984
BstHHI	GCGC	1 cut(s)	1056
BstKTI	GATC	7 cut(s)	486, 531, 681, 705, 1183, 1191, 1566
BstMAI	GTCTC	5 cut(s)	136, 213, 621, 687, 1345
BstMBI	GATC	7 cut(s)	483, 528, 678, 702, 1180, 1188, 1563
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 193, 635, 872, 1151, 1529, 1535, 1538
BstNI	CCWGG	4 cut(s)	1061, 1076, 1572, 1625
BstNSI	RCATGY	1 cut(s)	253
BstPAI	GACNNNNGTC	1 cut(s)	1508
BstSCI	CCNGG	4 cut(s)	1059, 1074, 1570, 1623
BstSFI	CTRYAG	2 cut(s)	873, 1524
BstSLI	GKGCMC	2 cut(s)	168, 549
BstUI	CGCG	1 cut(s)	984
BstV1I	GCAGC	6 cut(s)	159, 205, 736, 887, 1516, 1550
BstV2I	GAAGAC	1 cut(s)	1077
BstX2I	RGATCY	1 cut(s)	678
BstYI	RGATCY	1 cut(s)	678
Bsu15I	ATCGAT	1 cut(s)	1562
BsuRI	GGCC	1 cut(s)	50
BsuTUI	ATCGAT	1 cut(s)	1562
BtgI	CCRYGG	2 cut(s)	673, 796
BtsCI	GGATG	4 cut(s)	131, 465, 1104, 1513
BtsI	GCAGTG	2 cut(s)	21, 1191
BtsIMutI	CAGTG	6 cut(s)	15, 21, 1020, 1191, 1336, 1425
BveI	ACCTGC	1 cut(s)	1329
Cac8I	GCNNGC	2 cut(s)	48, 982
CciI	TCATGA	1 cut(s)	1605
CfoI	GCGC	1 cut(s)	1056
Cfr10I	RCCGGY	1 cut(s)	140
Cfr13I	GGNCC	6 cut(s)	138, 338, 571, 778, 800, 1381
ClaI	ATCGAT	1 cut(s)	1562
Csp6I	GTAC	6 cut(s)	36, 239, 285, 1274, 1318, 1460
CviAII	CATG	8 cut(s)	205, 250, 455, 674, 797, 1298, 1598, 1606
CviQI	GTAC	6 cut(s)	36, 239, 285, 1274, 1318, 1460
DdeI	CTNAG	6 cut(s)	133, 183, 404, 711, 717, 1385
DpnI	GATC	7 cut(s)	485, 530, 680, 704, 1182, 1190, 1565
DpnII	GATC	7 cut(s)	483, 528, 678, 702, 1180, 1188, 1563
Eam1104I	CTCTTC	2 cut(s)	379, 1590
EarI	CTCTTC	2 cut(s)	379, 1590
Ecl136II	GAGCTC	2 cut(s)	607, 1583
Eco130I	CCWWGG	3 cut(s)	81, 673, 796
Eco147I	AGGCCT	1 cut(s)	50
Eco24I	GRGCYC	2 cut(s)	609, 1585
Eco31I	GGTCTC	1 cut(s)	136
Eco32I	GATATC	1 cut(s)	93
Eco47I	GGWCC	6 cut(s)	138, 338, 571, 778, 800, 1381
Eco53kI	GAGCTC	2 cut(s)	607, 1583
Eco57I	CTGAAG	2 cut(s)	864, 1044
Eco88I	CYCGRG	1 cut(s)	188
EcoICRI	GAGCTC	2 cut(s)	607, 1583
EcoO109I	RGGNCCY	1 cut(s)	1381
EcoRII	CCWGG	4 cut(s)	1059, 1074, 1570, 1623
EcoRV	GATATC	1 cut(s)	93
EcoT14I	CCWWGG	3 cut(s)	81, 673, 796
EcoT38I	GRGCYC	2 cut(s)	609, 1585
ErhI	CCWWGG	3 cut(s)	81, 673, 796
FaeI	CATG	8 cut(s)	208, 253, 458, 677, 800, 1301, 1601, 1609
FaqI	GGGAC	5 cut(s)	151, 557, 883, 946, 1394
FatI	CATG	8 cut(s)	204, 249, 454, 673, 796, 1297, 1597, 1605
FauI	CCCGC	1 cut(s)	989
FauNDI	CATATG	4 cut(s)	418, 519, 976, 1015
FbaI	TGATCA	2 cut(s)	483, 528
Fnu4HI	GCNGC	8 cut(s)	106, 145, 148, 194, 750, 876, 1530, 1539
FokI	GGATG	4 cut(s)	138, 472, 1091, 1520
FriOI	GRGCYC	2 cut(s)	609, 1585
Fsp4HI	GCNGC	8 cut(s)	106, 145, 148, 194, 750, 876, 1530, 1539
GlaI	GCGC	1 cut(s)	1055
GluI	GCNGC	8 cut(s)	106, 145, 148, 194, 750, 876, 1530, 1539
GsaI	CCCAGC	1 cut(s)	870
GsuI	CTGGAG	3 cut(s)	456, 1243, 1312
HaeIII	GGCC	1 cut(s)	50
HapII	CCGG	1 cut(s)	141
HhaI	GCGC	1 cut(s)	1056
Hin1I	GRCGYC	1 cut(s)	41
Hin1II	CATG	8 cut(s)	208, 253, 458, 677, 800, 1301, 1601, 1609
Hin6I	GCGC	1 cut(s)	1054
HinP1I	GCGC	1 cut(s)	1054
HincII	GTYRAC	1 cut(s)	1162
HindII	GTYRAC	1 cut(s)	1162
HindIII	AAGCTT	1 cut(s)	1143
HpaII	CCGG	1 cut(s)	141
HphI	GGTGA	2 cut(s)	82, 505
Hpy166II	GTNNAC	7 cut(s)	19, 166, 285, 341, 862, 1162, 1318
Hpy8I	GTNNAC	7 cut(s)	19, 166, 285, 341, 862, 1162, 1318
Hpy99I	CGWCG	4 cut(s)	43, 46, 173, 909
HpyAV	CCTTC	3 cut(s)	53, 220, 990
HpyCH4III	ACNGT	3 cut(s)	551, 1206, 1420
HpyCH4IV	ACGT	2 cut(s)	41, 907
HpyCH4V	TGCA	9 cut(s)	166, 497, 846, 875, 912, 1010, 1121, 1210, 1532
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 193, 635, 872, 1151, 1529, 1535, 1538
HpyF3I	CTNAG	6 cut(s)	133, 183, 404, 711, 717, 1385
HpySE526I	ACGT	2 cut(s)	41, 907
Hsp92I	GRCGYC	1 cut(s)	41
Hsp92II	CATG	8 cut(s)	208, 253, 458, 677, 800, 1301, 1601, 1609
HspAI	GCGC	1 cut(s)	1054
KpnI	GGTACC	1 cut(s)	242
Ksp22I	TGATCA	2 cut(s)	483, 528
Kzo9I	GATC	7 cut(s)	483, 528, 678, 702, 1180, 1188, 1563
LguI	GCTCTTC	1 cut(s)	1590
LmnI	GCTCC	2 cut(s)	437, 1580
Lsp1109I	GCAGC	6 cut(s)	159, 205, 736, 887, 1516, 1550
LweI	GCATC	4 cut(s)	14, 159, 414, 1108
MaeII	ACGT	2 cut(s)	41, 907
MaeIII	GTNAC	6 cut(s)	407, 475, 668, 955, 1364, 1501
MalI	GATC	7 cut(s)	485, 530, 680, 704, 1182, 1190, 1565
MboI	GATC	7 cut(s)	483, 528, 678, 702, 1180, 1188, 1563
MboII	GAAGA	6 cut(s)	364, 396, 1037, 1082, 1198, 1577
MflI	RGATCY	1 cut(s)	678
MhlI	GDGCHC	4 cut(s)	168, 549, 609, 1585
MluCI	AATT	5 cut(s)	503, 890, 1247, 1372, 1455
MlyI	GAGTC	5 cut(s)	167, 623, 689, 709, 1157
MmeI	TCCRAC	4 cut(s)	120, 973, 1450, 1591
MnlI	CCTC	9 cut(s)	110, 238, 327, 360, 922, 1120, 1123, 1126, 1483
MseI	TTAA	1 cut(s)	1287
MslI	CAYNNNNRTG	6 cut(s)	254, 453, 518, 857, 1313, 1418
MspCI	CTTAAG	1 cut(s)	1286
MspI	CCGG	1 cut(s)	141
MspR9I	CCNGG	4 cut(s)	1061, 1076, 1572, 1625
MvaI	CCWGG	4 cut(s)	1061, 1076, 1572, 1625
MvnI	CGCG	1 cut(s)	984
MwoI	GCNNNNNNNGC	8 cut(s)	11, 193, 635, 872, 1151, 1529, 1535, 1538
NcoI	CCATGG	2 cut(s)	673, 796
NdeI	CATATG	4 cut(s)	418, 519, 976, 1015
NdeII	GATC	7 cut(s)	483, 528, 678, 702, 1180, 1188, 1563
NlaIII	CATG	8 cut(s)	208, 253, 458, 677, 800, 1301, 1601, 1609
NlaIV	GGNNCC	5 cut(s)	139, 240, 573, 780, 1382
NmuCI	GTSAC	2 cut(s)	955, 1501
NspI	RCATGY	1 cut(s)	253
NspV	TTCGAA	1 cut(s)	508
OliI	CACNNNNGTG	1 cut(s)	1418
PagI	TCATGA	1 cut(s)	1605
PceI	AGGCCT	1 cut(s)	50
PciI	ACATGT	1 cut(s)	249
PciSI	GCTCTTC	1 cut(s)	1590
PfeI	GAWTC	6 cut(s)	400, 464, 510, 537, 1301, 1594
PflMI	CCANNNNNTGG	2 cut(s)	797, 1577
PfoI	TCCNGGA	1 cut(s)	1623
PkrI	GCNGC	8 cut(s)	107, 146, 149, 195, 751, 877, 1531, 1540
PleI	GAGTC	5 cut(s)	167, 622, 689, 709, 1157
PpsI	GAGTC	5 cut(s)	167, 622, 689, 709, 1157
PpuMI	RGGWCCY	1 cut(s)	1381
PscI	ACATGT	1 cut(s)	249
PshAI	GACNNNNGTC	1 cut(s)	1508
Psp124BI	GAGCTC	2 cut(s)	609, 1585
Psp5II	RGGWCCY	1 cut(s)	1381
Psp6I	CCWGG	4 cut(s)	1059, 1074, 1570, 1623
PspFI	CCCAGC	1 cut(s)	866
PspGI	CCWGG	4 cut(s)	1059, 1074, 1570, 1623
PspN4I	GGNNCC	5 cut(s)	139, 240, 573, 780, 1382
PspPI	GGNCC	6 cut(s)	138, 338, 571, 778, 800, 1381
PspPPI	RGGWCCY	1 cut(s)	1381
PstI	CTGCAG	1 cut(s)	877
PsuI	RGATCY	1 cut(s)	678
RsaI	GTAC	6 cut(s)	37, 240, 286, 1275, 1319, 1461
RsaNI	GTAC	6 cut(s)	36, 239, 285, 1274, 1318, 1460
RseI	CAYNNNNRTG	6 cut(s)	254, 453, 518, 857, 1313, 1418
SacI	GAGCTC	2 cut(s)	609, 1585
SapI	GCTCTTC	1 cut(s)	1590
SaqAI	TTAA	1 cut(s)	1287
SatI	GCNGC	8 cut(s)	106, 145, 148, 194, 750, 876, 1530, 1539
Sau3AI	GATC	7 cut(s)	483, 528, 678, 702, 1180, 1188, 1563
Sau96I	GGNCC	6 cut(s)	138, 338, 571, 778, 800, 1381
SchI	GAGTC	5 cut(s)	167, 623, 689, 709, 1157
ScrFI	CCNGG	4 cut(s)	1061, 1076, 1572, 1625
SduI	GDGCHC	4 cut(s)	168, 549, 609, 1585
SfaNI	GCATC	4 cut(s)	14, 159, 414, 1108
SfcI	CTRYAG	2 cut(s)	873, 1524
SfuI	TTCGAA	1 cut(s)	508
SinI	GGWCC	6 cut(s)	138, 338, 571, 778, 800, 1381
SmiMI	CAYNNNNRTG	6 cut(s)	254, 453, 518, 857, 1313, 1418
SmlI	CTYRAG	2 cut(s)	1032, 1286
SmoI	CTYRAG	2 cut(s)	1032, 1286
Sse9I	AATT	5 cut(s)	503, 890, 1247, 1372, 1455
SseBI	AGGCCT	1 cut(s)	50
SsiI	CCGC	4 cut(s)	68, 106, 144, 982
SspI	AATATT	1 cut(s)	1394
SstI	GAGCTC	2 cut(s)	609, 1585
StuI	AGGCCT	1 cut(s)	50
StyD4I	CCNGG	4 cut(s)	1059, 1074, 1570, 1623
StyI	CCWWGG	3 cut(s)	81, 673, 796
TaaI	ACNGT	3 cut(s)	551, 1206, 1420
TaiI	ACGT	2 cut(s)	44, 910
TaqI	TCGA	5 cut(s)	508, 927, 1166, 1266, 1562
TasI	AATT	5 cut(s)	503, 890, 1247, 1372, 1455
TatI	WGTACW	3 cut(s)	284, 1317, 1459
TauI	GCSGC	2 cut(s)	108, 147
TfiI	GAWTC	6 cut(s)	400, 464, 510, 537, 1301, 1594
Tru1I	TTAA	1 cut(s)	1287
Tru9I	TTAA	1 cut(s)	1287
TscAI	CASTG	6 cut(s)	15, 21, 1027, 1198, 1336, 1425
TseFI	GTSAC	2 cut(s)	955, 1501
TseI	GCWGC	6 cut(s)	147, 193, 749, 875, 1529, 1538
Tsp45I	GTSAC	2 cut(s)	955, 1501
TspDTI	ATGAA	6 cut(s)	365, 437, 612, 752, 1260, 1594
TspGWI	ACGGA	2 cut(s)	36, 1520
TspRI	CASTG	6 cut(s)	15, 21, 1027, 1198, 1336, 1425
Van91I	CCANNNNNTGG	2 cut(s)	797, 1577
Vha464I	CTTAAG	1 cut(s)	1286
VneI	GTGCAC	1 cut(s)	164
VpaK11BI	GGWCC	6 cut(s)	138, 338, 571, 778, 800, 1381
XapI	RAATTY	2 cut(s)	503, 890
XceI	RCATGY	1 cut(s)	253
ZraI	GACGTC	1 cut(s)	42

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.