Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0010004

Physical Location & Seq

Genomic Coordinates Forward (+)

32266499 .. 32272209

Gene Structure 5711 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1237500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 897 bp

ATGATACCTATCCAGTGCGATCTCTGGATCACTACTGAATGCGGAAAGTTGCAAGTTTCTCCTGGTGAAATTGCTGTATTACCTCAAGGATTTCGTTTTGCGGTTGATCTGCCTGATGGCCCTTCGCGTGGTTATGTGGCCGAGGTTTTCGGCACCCATTTTCAACTCCCTGATCTTGGACCAATTGGAGCTAATGGTCTCGCTGCTCCAAGGGATTTTCTTGTTCCCACTGCGTGGTTCGAAGAGAGTTCCCGACCCGGCTACATCATTATACAGAAATTTGGTGGTGAACTCTTTACTGCAAAGCAAGATTTCTCTCCCTTCAATGTAGTTGCTTGGCATGGCAATTATACTCCATATAAGTATGATCTGACTACGTTCTGCCCCTTTAACACTGTTTTATTTGATCACGGTGATCCATCAGTGAATACAGTTTTGACAGCACCAACTGATAAACCTGGTGTTGCATTACTTGATTTCGTCATTTTCCCTCCGCGATGGTTGGTTGCTGAGCATACATTCCGACCTCCATACTATCATCGTAATTGTATGAGTGAGTTTATGGGTCTCATCTACGGTGGATATGAGGCAAAAGCGGATGGTTTTCTTCCTGGTGGTGCAAGCCTTCATAGCTGCATGACTCCCCATGGTCCTGATACCAAAACATATGAGGCTACCATTGCGCGTGGAAATGAGGCAGGACCATTCAGAATCACCGATACTTTGGCTTTTATGTTTGAATCGTGTTTAATCCCCAGAGTATGCACATGGGCTCTCGAGTCTCCAGTCATCGATCGTGATTATTACCAGTGCTGGACTGGACTGAAATCACATTTTACGCGTGGAAGGATGAATGCTGAAGACGGAGATGTCCAGAATGGGAAGAAAGAGGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004411 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006520 GO:0006570 GO:0006572 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009072 GO:0009074 GO:0009987 GO:0016054 GO:0016491 GO:0016701 GO:0016702 GO:0018958 GO:0019336 GO:0019439 GO:0019752 GO:0032787 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0051213 GO:0055114 GO:0071704 GO:0072329 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606 GO:1901615 GO:1901616 GO:1901999 GO:1902000

KEGG Pathways

Metabolic & Signaling

ko00350 ko00643 ko01100 ko01120 map00350 map00643 map01100 map01120

Pfam Domains

Protein Families

HgmA

Protein Analysis

299

Amino Acids

33.17

Weight (kDa)

5.15

Isoelectric Point (pI)

39.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HgmA_N	PF20510	2 - 124	6e-58	Homogentisate 1,2-dioxygenase N-terminal
HgmA_C	PF04209	126 - 279	1.8e-76	Homogentisate 1,2-dioxygenase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010004)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54080 AT5G54080
fragaria_vesca	FvH4_3g19020 FvH4_3g19020 FvH4_3g19020
malus_domestica	MD03G1237300.v1.1 MD03G1237500.v1.1
prunus_persica	Prupe.4G172100_v2.0.a1
pyrus_communis	pycom03g18320
rosa_chinensis	RchiOBHm_Chr5g0031821
rosa_laevigata	RLG00000033349
rosa_multiflora	Rmu_co8454513.1_g000001 Rmu_sc0006847.1_g000029
rosa_roxburghii	Rroxscaffold_1G00048450
rosa_rugosa	Rorug05G0127800 Rorug05G0127900
rosa_samantha	Rh5AG222100 Rh5BG222100 Rh5CG245400 Rh5DG227000
rosa_wichuraiana	Rw5G020250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	869
AccB1I	GGYRCC	1 cut(s)	152
AccB7I	CCANNNNNTGG	1 cut(s)	234
AccII	CGCG	4 cut(s)	127, 496, 685, 841
AciI	CCGC	4 cut(s)	42, 101, 494, 596
AclWI	GGATC	2 cut(s)	35, 410
AcoI	YGGCCR	1 cut(s)	138
AcsI	RAATTY	1 cut(s)	278
AcuI	CTGAAG	1 cut(s)	879
AdeI	CACNNNGTG	1 cut(s)	234
AfiI	CCNNNNNNNGG	3 cut(s)	128, 176, 234
AflIII	ACRYGT	1 cut(s)	839
AgsI	TTSAA	3 cut(s)	164, 325, 740
AjnI	CCWGG	3 cut(s)	61, 457, 610
AluBI	AGCT	2 cut(s)	191, 633
AluI	AGCT	2 cut(s)	191, 633
Alw26I	GTCTC	3 cut(s)	203, 572, 786
AlwI	GGATC	2 cut(s)	35, 410
Ama87I	CYCGRG	1 cut(s)	776
AoxI	GGCC	2 cut(s)	118, 138
ApeKI	GCWGC	2 cut(s)	203, 633
ApoI	RAATTY	1 cut(s)	278
AspLEI	GCGC	1 cut(s)	685
AspS9I	GGNCC	4 cut(s)	119, 179, 650, 701
AsuC2I	CCSGG	1 cut(s)	258
AsuHPI	GGTGA	4 cut(s)	77, 299, 425, 706
AsuII	TTCGAA	1 cut(s)	240
AvaI	CYCGRG	1 cut(s)	776
AvaII	GGWCC	3 cut(s)	179, 650, 701
BanI	GGYRCC	1 cut(s)	152
BanII	GRGCYC	1 cut(s)	775
BbsI	GAAGAC	1 cut(s)	867
BbvI	GCAGC	2 cut(s)	190, 620
BccI	CCATC	4 cut(s)	110, 427, 492, 593
BciT130I	CCWGG	3 cut(s)	63, 459, 612
BclI	TGATCA	1 cut(s)	406
BcnI	CCSGG	1 cut(s)	258
BcoDI	GTCTC	3 cut(s)	203, 572, 786
BisI	GCNGC	2 cut(s)	204, 634
BlpI	GCTNAGC	1 cut(s)	510
BlsI	GCNGC	2 cut(s)	205, 635
Bme1390I	CCNGG	4 cut(s)	63, 258, 459, 612
Bme18I	GGWCC	3 cut(s)	179, 650, 701
BmeT110I	CYCGRG	1 cut(s)	776
BmgT120I	GGNCC	4 cut(s)	119, 179, 650, 701
BmiI	GGNNCC	1 cut(s)	154
BmrFI	CCNGG	4 cut(s)	63, 258, 459, 612
BpiI	GAAGAC	1 cut(s)	867
BpmI	CTGGAG	1 cut(s)	768
Bpu1102I	GCTNAGC	1 cut(s)	510
Bpu14I	TTCGAA	1 cut(s)	240
BpuEI	CTTGAG	1 cut(s)	69
BpuMI	CCSGG	1 cut(s)	258
Bsa29I	ATCGAT	1 cut(s)	792
BsaBI	GATNNNNATC	1 cut(s)	8
BsaI	GGTCTC	2 cut(s)	203, 572
BsaJI	CCNNGG	3 cut(s)	141, 209, 646
Bsc4I	CCNNNNNNNGG	3 cut(s)	128, 176, 234
Bse1I	ACTGG	4 cut(s)	13, 785, 808, 823
Bse3DI	GCAATG	1 cut(s)	678
Bse8I	GATNNNNATC	1 cut(s)	8
BseBI	CCWGG	3 cut(s)	63, 459, 612
BseCI	ATCGAT	1 cut(s)	792
BseDI	CCNNGG	3 cut(s)	141, 209, 646
BseGI	GGATG	2 cut(s)	604, 855
BseJI	GATNNNNATC	1 cut(s)	8
BseLI	CCNNNNNNNGG	3 cut(s)	128, 176, 234
BseMI	GCAATG	1 cut(s)	678
BseMII	CTCAG	1 cut(s)	501
BseNI	ACTGG	4 cut(s)	13, 785, 808, 823
BseXI	GCAGC	2 cut(s)	190, 620
Bsh1236I	CGCG	4 cut(s)	127, 496, 685, 841
Bsh1285I	CGRYCG	1 cut(s)	796
BshFI	GGCC	2 cut(s)	120, 140
BshNI	GGYRCC	1 cut(s)	152
BshVI	ATCGAT	1 cut(s)	792
BsiEI	CGRYCG	1 cut(s)	796
BsiHKCI	CYCGRG	1 cut(s)	776
BsiSI	CCGG	1 cut(s)	258
BslI	CCNNNNNNNGG	3 cut(s)	128, 176, 234
BsmAI	GTCTC	3 cut(s)	203, 572, 786
BsmI	GAATGC	2 cut(s)	44, 859
BsnI	GGCC	2 cut(s)	120, 140
Bso31I	GGTCTC	2 cut(s)	203, 572
BsoBI	CYCGRG	1 cut(s)	776
Bsp119I	TTCGAA	1 cut(s)	240
Bsp1286I	GDGCHC	1 cut(s)	775
Bsp143I	GATC	8 cut(s)	19, 27, 106, 172, 367, 406, 415, 793
Bsp1720I	GCTNAGC	1 cut(s)	510
Bsp19I	CCATGG	1 cut(s)	646
BspACI	CCGC	4 cut(s)	42, 101, 494, 596
BspANI	GGCC	2 cut(s)	120, 140
BspCNI	CTCAG	1 cut(s)	502
BspDI	ATCGAT	1 cut(s)	792
BspFNI	CGCG	4 cut(s)	127, 496, 685, 841
BspLI	GGNNCC	1 cut(s)	154
BspPI	GGATC	2 cut(s)	35, 410
BspT104I	TTCGAA	1 cut(s)	240
BspT107I	GGYRCC	1 cut(s)	152
BspTNI	GGTCTC	2 cut(s)	203, 572
BsrDI	GCAATG	1 cut(s)	678
BsrI	ACTGG	4 cut(s)	13, 785, 808, 823
BssECI	CCNNGG	3 cut(s)	141, 209, 646
BssMI	GATC	8 cut(s)	19, 27, 106, 172, 367, 406, 415, 793
BssT1I	CCWWGG	2 cut(s)	209, 646
Bst2UI	CCWGG	3 cut(s)	63, 459, 612
Bst4CI	ACNGT	4 cut(s)	397, 413, 433, 578
Bst6I	CTCTTC	1 cut(s)	237
BstBI	TTCGAA	1 cut(s)	240
BstC8I	GCNNGC	1 cut(s)	622
BstDEI	CTNAG	1 cut(s)	510
BstDSI	CCRYGG	1 cut(s)	646
BstF5I	GGATG	2 cut(s)	604, 855
BstFNI	CGCG	4 cut(s)	127, 496, 685, 841
BstHHI	GCGC	1 cut(s)	685
BstKTI	GATC	8 cut(s)	22, 30, 109, 175, 370, 409, 418, 796
BstMAI	GTCTC	3 cut(s)	203, 572, 786
BstMBI	GATC	8 cut(s)	19, 27, 106, 172, 367, 406, 415, 793
BstMCI	CGRYCG	1 cut(s)	796
BstMWI	GCNNNNNNNGC	2 cut(s)	630, 680
BstNI	CCWGG	3 cut(s)	63, 459, 612
BstSCI	CCNGG	4 cut(s)	61, 256, 457, 610
BstUI	CGCG	4 cut(s)	127, 496, 685, 841
BstV1I	GCAGC	2 cut(s)	190, 620
BstV2I	GAAGAC	1 cut(s)	867
Bsu15I	ATCGAT	1 cut(s)	792
BsuRI	GGCC	2 cut(s)	120, 140
BsuTUI	ATCGAT	1 cut(s)	792
BtgI	CCRYGG	1 cut(s)	646
BtgZI	GCGATG	1 cut(s)	511
BtsCI	GGATG	2 cut(s)	604, 855
BtsI	GCAGTG	1 cut(s)	228
BtsIMutI	CAGTG	5 cut(s)	20, 228, 393, 429, 815
Cac8I	GCNNGC	1 cut(s)	622
CfoI	GCGC	1 cut(s)	685
Cfr13I	GGNCC	4 cut(s)	119, 179, 650, 701
ClaI	ATCGAT	1 cut(s)	792
CsiI	ACCWGGT	1 cut(s)	457
CviAII	CATG	4 cut(s)	341, 637, 647, 768
CviJI	RGCY	9 cut(s)	120, 140, 191, 261, 624, 633, 674, 728, 773
CviKI_1	RGCY	9 cut(s)	120, 140, 191, 261, 624, 633, 674, 728, 773
DdeI	CTNAG	1 cut(s)	510
DpnI	GATC	8 cut(s)	21, 29, 108, 174, 369, 408, 417, 795
DpnII	GATC	8 cut(s)	19, 27, 106, 172, 367, 406, 415, 793
DraIII	CACNNNGTG	1 cut(s)	234
DrdI	GACNNNNNNGTC	1 cut(s)	869
DseDI	GACNNNNNNGTC	1 cut(s)	869
EaeI	YGGCCR	1 cut(s)	138
Eam1104I	CTCTTC	1 cut(s)	237
EarI	CTCTTC	1 cut(s)	237
Eco130I	CCWWGG	2 cut(s)	209, 646
Eco24I	GRGCYC	1 cut(s)	775
Eco31I	GGTCTC	2 cut(s)	203, 572
Eco47I	GGWCC	3 cut(s)	179, 650, 701
Eco57I	CTGAAG	1 cut(s)	879
Eco88I	CYCGRG	1 cut(s)	776
EcoRII	CCWGG	3 cut(s)	61, 457, 610
EcoT14I	CCWWGG	2 cut(s)	209, 646
EcoT38I	GRGCYC	1 cut(s)	775
ErhI	CCWWGG	2 cut(s)	209, 646
FaeI	CATG	4 cut(s)	344, 640, 650, 771
FatI	CATG	4 cut(s)	340, 636, 646, 767
FauNDI	CATATG	1 cut(s)	667
FbaI	TGATCA	1 cut(s)	406
Fnu4HI	GCNGC	2 cut(s)	204, 634
FokI	GGATG	2 cut(s)	611, 862
FriOI	GRGCYC	1 cut(s)	775
Fsp4HI	GCNGC	2 cut(s)	204, 634
GlaI	GCGC	1 cut(s)	684
GluI	GCNGC	2 cut(s)	204, 634
GsuI	CTGGAG	1 cut(s)	768
HaeIII	GGCC	2 cut(s)	120, 140
HapII	CCGG	1 cut(s)	258
HhaI	GCGC	1 cut(s)	685
Hin1II	CATG	4 cut(s)	344, 640, 650, 771
Hin6I	GCGC	1 cut(s)	683
HinP1I	GCGC	1 cut(s)	683
HinfI	GANTC	4 cut(s)	640, 711, 740, 779
HpaII	CCGG	1 cut(s)	258
HphI	GGTGA	4 cut(s)	77, 299, 425, 706
Hpy166II	GTNNAC	1 cut(s)	290
Hpy188I	TCNGA	3 cut(s)	372, 524, 710
Hpy188III	TCNNGA	6 cut(s)	25, 252, 653, 776, 797, 874
Hpy8I	GTNNAC	1 cut(s)	290
HpyAV	CCTTC	4 cut(s)	132, 331, 635, 840
HpyCH4III	ACNGT	4 cut(s)	397, 413, 433, 578
HpyCH4IV	ACGT	1 cut(s)	377
HpyCH4V	TGCA	6 cut(s)	52, 302, 467, 620, 636, 765
HpyF10VI	GCNNNNNNNGC	2 cut(s)	630, 680
HpyF3I	CTNAG	1 cut(s)	510
HpySE526I	ACGT	1 cut(s)	377
Hsp92II	CATG	4 cut(s)	344, 640, 650, 771
HspAI	GCGC	1 cut(s)	683
Ksp22I	TGATCA	1 cut(s)	406
Kzo9I	GATC	8 cut(s)	19, 27, 106, 172, 367, 406, 415, 793
LmnI	GCTCC	2 cut(s)	188, 211
Lsp1109I	GCAGC	2 cut(s)	190, 620
MabI	ACCWGGT	1 cut(s)	457
MaeII	ACGT	1 cut(s)	377
MalI	GATC	8 cut(s)	21, 29, 108, 174, 369, 408, 417, 795
MboI	GATC	8 cut(s)	19, 27, 106, 172, 367, 406, 415, 793
MboII	GAAGA	4 cut(s)	254, 599, 872, 895
MfeI	CAATTG	1 cut(s)	183
MhlI	GDGCHC	1 cut(s)	775
MluCI	AATT	5 cut(s)	69, 183, 278, 346, 544
MluI	ACGCGT	1 cut(s)	839
MlyI	GAGTC	2 cut(s)	634, 788
MmeI	TCCRAC	1 cut(s)	547
MnlI	CCTC	8 cut(s)	93, 136, 501, 537, 580, 664, 688, 883
MseI	TTAA	3 cut(s)	390, 749, 895
MspI	CCGG	1 cut(s)	258
MspR9I	CCNGG	4 cut(s)	63, 258, 459, 612
MunI	CAATTG	1 cut(s)	183
Mva1269I	GAATGC	2 cut(s)	44, 859
MvaI	CCWGG	3 cut(s)	63, 459, 612
MvnI	CGCG	4 cut(s)	127, 496, 685, 841
MwoI	GCNNNNNNNGC	2 cut(s)	630, 680
NciI	CCSGG	1 cut(s)	258
NcoI	CCATGG	1 cut(s)	646
NdeI	CATATG	1 cut(s)	667
NdeII	GATC	8 cut(s)	19, 27, 106, 172, 367, 406, 415, 793
NlaIII	CATG	4 cut(s)	344, 640, 650, 771
NlaIV	GGNNCC	1 cut(s)	154
NmeAIII	GCCGAG	1 cut(s)	166
NspV	TTCGAA	1 cut(s)	240
PaeR7I	CTCGAG	1 cut(s)	776
PctI	GAATGC	2 cut(s)	44, 859
PfeI	GAWTC	2 cut(s)	711, 740
PflMI	CCANNNNNTGG	1 cut(s)	234
PkrI	GCNGC	2 cut(s)	205, 635
Ple19I	CGATCG	1 cut(s)	796
PleI	GAGTC	2 cut(s)	634, 787
PpsI	GAGTC	2 cut(s)	634, 787
Psp6I	CCWGG	3 cut(s)	61, 457, 610
PspGI	CCWGG	3 cut(s)	61, 457, 610
PspN4I	GGNNCC	1 cut(s)	154
PspPI	GGNCC	4 cut(s)	119, 179, 650, 701
PvuI	CGATCG	1 cut(s)	796
SaqAI	TTAA	3 cut(s)	390, 749, 895
SatI	GCNGC	2 cut(s)	204, 634
Sau3AI	GATC	8 cut(s)	19, 27, 106, 172, 367, 406, 415, 793
Sau96I	GGNCC	4 cut(s)	119, 179, 650, 701
SchI	GAGTC	2 cut(s)	634, 788
ScrFI	CCNGG	4 cut(s)	63, 258, 459, 612
SduI	GDGCHC	1 cut(s)	775
SetI	ASST	8 cut(s)	10, 85, 147, 193, 380, 460, 529, 635
SexAI	ACCWGGT	1 cut(s)	457
Sfr274I	CTCGAG	1 cut(s)	776
SfuI	TTCGAA	1 cut(s)	240
SinI	GGWCC	3 cut(s)	179, 650, 701
SlaI	CTCGAG	1 cut(s)	776
SmlI	CTYRAG	2 cut(s)	84, 776
SmoI	CTYRAG	2 cut(s)	84, 776
Sse9I	AATT	5 cut(s)	69, 183, 278, 346, 544
SsiI	CCGC	4 cut(s)	42, 101, 494, 596
StyD4I	CCNGG	4 cut(s)	61, 256, 457, 610
StyI	CCWWGG	2 cut(s)	209, 646
TaaI	ACNGT	4 cut(s)	397, 413, 433, 578
TaiI	ACGT	1 cut(s)	380
TaqI	TCGA	3 cut(s)	240, 777, 792
TasI	AATT	5 cut(s)	69, 183, 278, 346, 544
TfiI	GAWTC	2 cut(s)	711, 740
Tru1I	TTAA	3 cut(s)	390, 749, 895
Tru9I	TTAA	3 cut(s)	390, 749, 895
TscAI	CASTG	5 cut(s)	20, 235, 400, 429, 815
TseI	GCWGC	2 cut(s)	203, 633
TspDTI	ATGAA	2 cut(s)	617, 866
TspGWI	ACGGA	1 cut(s)	879
TspRI	CASTG	5 cut(s)	20, 235, 400, 429, 815
Van91I	CCANNNNNTGG	1 cut(s)	234
VpaK11BI	GGWCC	3 cut(s)	179, 650, 701
XapI	RAATTY	1 cut(s)	278
XcmI	CCANNNNNNNNNTGG	1 cut(s)	815
XhoI	CTCGAG	1 cut(s)	776

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.