MD03G1256100.v1.1

Histone H2A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010719
Physical Location & Seq
Forward (+)
34362959 .. 34364721
1763 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1256100.v1.1.491

Sequence Viewer

Length: 438 bp
ATGAATTTTTCTGAATATTGGGTAGAAATGGCGGGCAAGGGCGGGAAGGGGCTTATAGCAGCGAAAACCACAGCAGCTAACAAGGACAAGGACAAGGACAAGAAGAGGCCTGTTTCTCGTTCATCTCGCGCTGGTATCCAGTTTCCGGTGGGTCGAATTCATCGGCAGCTGAAACAGCGAATTGCTGCTCATGGGCGTGTTGGCGCCACTGCTGCTGTGTACTTGGCCTCAATCCTCGAGTACCTGACTGCTGAGGTTCTTGAGTTGGCCGGAAATGCTAGCAAGGATCTCAAGGTCAAGAGAATCACACCGAGGCATCTGCAACTGGCCATCAGGGGAGATGAGGAGCTGGACACTCTGATCAAAGGAACCATTGCCGGAGGTGGTGTCATCCCCCACATCCACAAGTCCCTCATCAACAAGACCTCCAAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006325 GO:0006342 GO:0006355 GO:0006996 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0040029 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071840 GO:0080090 GO:0097159 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04014 ko04015 ko04016 ko04020 ko04022 ko04024 ko04070 ko04114 ko04217 ko04218 ko04261 ko04270 ko04371 ko04626 ko04713 ko04720 ko04722 ko04728 ko04740 ko04744 ko04745 ko04750 ko04910 ko04912 ko04915 ko04916 ko04921 ko04922 ko04924 ko04925 ko04970 ko04971 ko05010 ko05031 ko05034 ko05133 ko05152 ko05167 ko05200 ko05214 ko05322 ko05418 map04014 map04015 map04016 map04020 map04022 map04024 map04070 map04114 map04217 map04218 map04261 map04270 map04371 map04626 map04713 map04720 map04722 map04728 map04740 map04744 map04745 map04750 map04910 map04912 map04915 map04916 map04921 map04922 map04924 map04925 map04970 map04971 map05010 map05031 map05034 map05133 map05152 map05167 map05200 map05214 map05322 map05418
Pfam Domains
Protein Families
EF-hand_7 Histone Histone_H2A_C

Protein Analysis

146

Amino Acids

15.76

Weight (kDa)

10.13

Isoelectric Point (pI)

33.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 28 - 113 1.9e-21 Core histone H2A/H2B/H3/H4 domain
Histone_H2A_C PF16211 115 - 144 1.7e-10 C-terminus of histone H2A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010719)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38810 AT2G38810 AT2G38810 AT2G38810
fragaria_vesca FvH4_2g22780
malus_domestica MD03G1256100.v1.1 MD11G1276900.v1.1
prunus_persica Prupe.8G233200_v2.0.a1 Prupe.8G233200_v2.0.a1
pyrus_communis pycom11g24490
rosa_chinensis RchiOBHm_Chr6g0289701
rosa_laevigata RLG00000012274
rosa_multiflora Rmu_sc0005640.1_g000031
rosa_roxburghii Rroxscaffold_7G00177640 Rroxscaffold_7G00178130
rosa_rugosa Rorug06G0205800
rosa_samantha Rh6AG317500 Rh6BG325100 Rh6DG316400
rosa_wichuraiana Rw6G027380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 203
AccII CGCG 1 cut(s) 129
AciI CCGC 2 cut(s) 32, 42
AclWI GGATC 1 cut(s) 294
AcoI YGGCCR 2 cut(s) 267, 327
AcsI RAATTY 2 cut(s) 4, 156
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 2 cut(s) 221, 242
AfiI CCNNNNNNNGG 1 cut(s) 145
AluBI AGCT 3 cut(s) 77, 169, 349
AluI AGCT 3 cut(s) 77, 169, 349
AlwI GGATC 1 cut(s) 294
Ama87I CYCGRG 1 cut(s) 236
AoxI GGCC 4 cut(s) 107, 225, 267, 327
ApeKI GCWGC 5 cut(s) 59, 74, 166, 185, 212
ApoI RAATTY 2 cut(s) 4, 156
AspLEI GCGC 2 cut(s) 131, 206
AsuNHI GCTAGC 1 cut(s) 278
AvaI CYCGRG 1 cut(s) 236
BalI TGGCCA 1 cut(s) 329
BanI GGYRCC 1 cut(s) 203
BbvCI CCTCAGC 1 cut(s) 252
BbvI GCAGC 5 cut(s) 71, 86, 172, 178, 199
BccI CCATC 1 cut(s) 338
BcgI CGANNNNNNTGC 2 cut(s) 301, 335
BciVI GTATCC 1 cut(s) 146
BclI TGATCA 1 cut(s) 360
BfaI CTAG 1 cut(s) 279
BfoI RGCGCY 1 cut(s) 207
BfuI GTATCC 1 cut(s) 146
BisI GCNGC 5 cut(s) 60, 75, 167, 186, 213
BlsI GCNGC 5 cut(s) 61, 76, 168, 187, 214
BmeT110I CYCGRG 1 cut(s) 236
BmiI GGNNCC 2 cut(s) 205, 370
BmsI GCATC 1 cut(s) 325
BmtI GCTAGC 1 cut(s) 282
Bpu10I CCTNAGC 1 cut(s) 252
BpuEI CTTGAG 2 cut(s) 275, 281
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 2 cut(s) 311, 429
BsaWI WCCGGW 1 cut(s) 145
BsaXI ACNNNNNCTCC 3 cut(s) 372, 402, 410
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse1I ACTGG 2 cut(s) 139, 330
Bse3DI GCAATG 1 cut(s) 372
BseDI CCNNGG 2 cut(s) 311, 429
BseGI GGATG 2 cut(s) 390, 399
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 1 cut(s) 243
BseNI ACTGG 2 cut(s) 139, 330
BseRI GAGGAG 1 cut(s) 359
BseXI GCAGC 5 cut(s) 71, 86, 172, 178, 199
Bsh1236I CGCG 1 cut(s) 129
BshFI GGCC 4 cut(s) 109, 227, 269, 329
BshNI GGYRCC 1 cut(s) 203
BsiHKCI CYCGRG 1 cut(s) 236
BsiSI CCGG 3 cut(s) 146, 270, 378
BslFI GGGAC 1 cut(s) 394
BslI CCNNNNNNNGG 1 cut(s) 145
BsmFI GGGAC 1 cut(s) 394
BsnI GGCC 4 cut(s) 109, 227, 269, 329
BsoBI CYCGRG 1 cut(s) 236
Bsp143I GATC 2 cut(s) 286, 360
BspACI CCGC 2 cut(s) 32, 42
BspANI GGCC 4 cut(s) 109, 227, 269, 329
BspCNI CTCAG 1 cut(s) 244
BspFNI CGCG 1 cut(s) 129
BspLI GGNNCC 2 cut(s) 205, 370
BspOI GCTAGC 1 cut(s) 282
BspPI GGATC 1 cut(s) 294
BspT107I GGYRCC 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 372
BsrI ACTGG 2 cut(s) 139, 330
BssECI CCNNGG 2 cut(s) 311, 429
BssMI GATC 2 cut(s) 286, 360
BssNI GRCGYC 1 cut(s) 204
BssT1I CCWWGG 1 cut(s) 429
Bst6I CTCTTC 1 cut(s) 98
BstACI GRCGYC 1 cut(s) 204
BstC8I GCNNGC 2 cut(s) 34, 280
BstDEI CTNAG 1 cut(s) 252
BstF5I GGATG 2 cut(s) 390, 399
BstFNI CGCG 1 cut(s) 129
BstH2I RGCGCY 1 cut(s) 207
BstHHI GCGC 2 cut(s) 131, 206
BstKTI GATC 2 cut(s) 289, 363
BstMBI GATC 2 cut(s) 286, 360
BstMWI GCNNNNNNNGC 3 cut(s) 175, 212, 275
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 5 cut(s) 71, 86, 172, 178, 199
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
BsuI GTATCC 1 cut(s) 146
BsuRI GGCC 4 cut(s) 109, 227, 269, 329
BtsCI GGATG 2 cut(s) 390, 399
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 207
Cac8I GCNNGC 2 cut(s) 34, 280
CfoI GCGC 2 cut(s) 131, 206
Csp6I GTAC 2 cut(s) 220, 241
CviAII CATG 1 cut(s) 191
CviJI RGCY 8 cut(s) 52, 77, 109, 169, 227, 269, 329, 349
CviKI_1 RGCY 8 cut(s) 52, 77, 109, 169, 227, 269, 329, 349
CviQI GTAC 2 cut(s) 220, 241
DdeI CTNAG 1 cut(s) 252
DinI GGCGCC 1 cut(s) 205
DpnI GATC 2 cut(s) 288, 362
DpnII GATC 2 cut(s) 286, 360
EaeI YGGCCR 2 cut(s) 267, 327
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
Eco130I CCWWGG 1 cut(s) 429
Eco147I AGGCCT 1 cut(s) 109
Eco88I CYCGRG 1 cut(s) 236
EcoRI GAATTC 1 cut(s) 156
EcoT14I CCWWGG 1 cut(s) 429
EgeI GGCGCC 1 cut(s) 205
EheI GGCGCC 1 cut(s) 205
ErhI CCWWGG 1 cut(s) 429
FaeI CATG 1 cut(s) 194
FaiI YATR 2 cut(s) 56, 192
FaqI GGGAC 1 cut(s) 394
FatI CATG 1 cut(s) 190
FauI CCCGC 2 cut(s) 25, 35
FbaI TGATCA 1 cut(s) 360
Fnu4HI GCNGC 5 cut(s) 60, 75, 167, 186, 213
FokI GGATG 2 cut(s) 377, 386
Fsp4HI GCNGC 5 cut(s) 60, 75, 167, 186, 213
FspBI CTAG 1 cut(s) 279
GlaI GCGC 2 cut(s) 130, 205
GluI GCNGC 5 cut(s) 60, 75, 167, 186, 213
HaeII RGCGCY 1 cut(s) 207
HaeIII GGCC 4 cut(s) 109, 227, 269, 329
HapII CCGG 3 cut(s) 146, 270, 378
HhaI GCGC 2 cut(s) 131, 206
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 1 cut(s) 194
Hin6I GCGC 2 cut(s) 129, 204
HinP1I GCGC 2 cut(s) 129, 204
HinfI GANTC 1 cut(s) 303
HpaII CCGG 3 cut(s) 146, 270, 378
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 2 cut(s) 13, 360
Hpy188III TCNNGA 2 cut(s) 260, 298
Hpy8I GTNNAC 1 cut(s) 220
HpyAV CCTTC 1 cut(s) 40
HpyCH4V TGCA 1 cut(s) 322
HpyF10VI GCNNNNNNNGC 3 cut(s) 175, 212, 275
HpyF3I CTNAG 1 cut(s) 252
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 1 cut(s) 194
HspAI GCGC 2 cut(s) 129, 204
KasI GGCGCC 1 cut(s) 203
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 2 cut(s) 286, 360
LmnI GCTCC 1 cut(s) 346
Lsp1109I GCAGC 5 cut(s) 71, 86, 172, 178, 199
LweI GCATC 1 cut(s) 325
MaeI CTAG 1 cut(s) 279
MalI GATC 2 cut(s) 288, 362
MboI GATC 2 cut(s) 286, 360
MboII GAAGA 1 cut(s) 115
MflI RGATCY 1 cut(s) 286
MlsI TGGCCA 1 cut(s) 329
MluCI AATT 3 cut(s) 4, 156, 180
MluNI TGGCCA 1 cut(s) 329
Mly113I GGCGCC 1 cut(s) 204
MnlI CCTC 9 cut(s) 99, 238, 245, 247, 306, 337, 374, 422, 436
Mox20I TGGCCA 1 cut(s) 329
MscI TGGCCA 1 cut(s) 329
MslI CAYNNNNRTG 1 cut(s) 195
Msp20I TGGCCA 1 cut(s) 329
MspA1I CMGCKG 1 cut(s) 169
MspI CCGG 3 cut(s) 146, 270, 378
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 3 cut(s) 175, 212, 275
NarI GGCGCC 1 cut(s) 204
NdeII GATC 2 cut(s) 286, 360
NheI GCTAGC 1 cut(s) 278
NlaIII CATG 1 cut(s) 194
NlaIV GGNNCC 2 cut(s) 205, 370
PaeR7I CTCGAG 1 cut(s) 236
PceI AGGCCT 1 cut(s) 109
PfeI GAWTC 1 cut(s) 303
PkrI GCNGC 5 cut(s) 61, 76, 168, 187, 214
PluTI GGCGCC 1 cut(s) 207
PspN4I GGNNCC 2 cut(s) 205, 370
PspXI VCTCGAGB 1 cut(s) 236
PsuI RGATCY 1 cut(s) 286
PvuII CAGCTG 1 cut(s) 169
RsaI GTAC 2 cut(s) 221, 242
RsaNI GTAC 2 cut(s) 220, 241
RseI CAYNNNNRTG 1 cut(s) 195
SatI GCNGC 5 cut(s) 60, 75, 167, 186, 213
Sau3AI GATC 2 cut(s) 286, 360
SetI ASST 8 cut(s) 79, 171, 246, 258, 297, 351, 385, 428
SfaNI GCATC 1 cut(s) 325
SfoI GGCGCC 1 cut(s) 205
Sfr274I CTCGAG 1 cut(s) 236
SlaI CTCGAG 1 cut(s) 236
SmiMI CAYNNNNRTG 1 cut(s) 195
SmlI CTYRAG 3 cut(s) 236, 260, 290
SmoI CTYRAG 3 cut(s) 236, 260, 290
Sse9I AATT 3 cut(s) 4, 156, 180
SseBI AGGCCT 1 cut(s) 109
SsiI CCGC 2 cut(s) 32, 42
SspDI GGCGCC 1 cut(s) 203
SspI AATATT 1 cut(s) 17
SspMI CTAG 1 cut(s) 279
StuI AGGCCT 1 cut(s) 109
StyI CCWWGG 1 cut(s) 429
TaqI TCGA 2 cut(s) 154, 237
TasI AATT 3 cut(s) 4, 156, 180
TatI WGTACW 1 cut(s) 219
TfiI GAWTC 1 cut(s) 303
TscAI CASTG 1 cut(s) 214
TseI GCWGC 5 cut(s) 59, 74, 166, 185, 212
TspDTI ATGAA 3 cut(s) 17, 111, 149
TspRI CASTG 1 cut(s) 214
XapI RAATTY 2 cut(s) 4, 156
XhoI CTCGAG 1 cut(s) 236
XspI CTAG 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.