MD03G1261800.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010100
Physical Location & Seq
Forward (+)
34793975 .. 34797527
3553 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1261800.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGTCTGAACAACTGATAGAAGGTCTCCCCGACGCTGTTGCCCTTAGGTGCCTTGCATGGGTTCCCTTCTATCTCCATCCCAAGTTGGAGCTTGTTTCTCGTTCCTGGCAAGCTGCCATTCATAGTGCTGAACTTTTTAGAGCTCGACAGGAAGTTGGCTCATGTGAGGATCTATTATGCGTGTCTGCTAATGAACCAGATAATTTATGGCAGCTATATGATCCTCGTCGAGACCTTTGGATTACACTCCCTGTTCTCCCCTCTAAAATAAAAAACCTTTCCAACTTCGGCACTGTCTCAACTGCTGGAAAACTGTTTGTTCTTGGTGGTGGCAGTGATGCTGTTGACCCACAGACTGGTGACCATGGTACCTTTTCAACCAATGAGGTTTGGTCATACGATCCTGTAATTAGGCGATGGAGCCCACGTGCATCTATGCTTGTTCCTCGTGCAATGTTTGCATGCTGTGTTTTAGGTGGAAAGATTGTTGTTGCAGGGGGTTTAACCAGCTGTCGGAAATCAATCTCTTTAGCAGAAATGTATGACCCTGATAACGATGTGTGGGCTCCAATTCCTGATCTACATTTATCTCATAATGCTGCATGCTCAGGGATAGTGATTGGAGAAAAGATGTACGTCTTGCACAGGGGTTTATCAACGGTGCAAGTCTTCGACAATGTGGGGCTTCAATGGACGGTAGAGGATTATGGTTGGCAACAGGGTCCAATGACAGTCATTAAGGGTGCACTTTATGTGATGAGCCATGGAGTTATCTCCAGGCAAGACAGAGAATCAGAGAAGGTATTGGTTTCGGCTTCTGAGTTTGGTGGCAGAGTTGGGTTTGCAATGGCTGGGCTAGGAGATGAAATTTATGTGATTGGTGGGGTGATCCTCTCTGAACATGGGACCTCTGAAATCAAGCAAATATCAGACGTTGATGTTCTCACGATCGGAGGTGAAAGACCAACTTGGCGCCAGGCAGCTCCGATGACACGGTGCAAAGGAAGTGTTTATGGTTGTGCACAGTTGAGAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

346

Amino Acids

37.75

Weight (kDa)

5.64

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 71 - 213 2.5e-07 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 77 - 212 7.7e-09 Calicin, beta-propeller domain
Kelch_1 PF01344 94 - 146 9.3e-06 Kelch motif
Kelch_FKB95 PF25210 126 - 264 4.8e-13 FKB95, Kelch-repeats domain
Kelch_1 PF01344 149 - 194 6.8e-11 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 368
AccB1I GGYRCC 3 cut(s) 48, 368, 972
AccB7I CCANNNNNTGG 1 cut(s) 356
AclWI GGATC 4 cut(s) 177, 215, 395, 883
AcsI RAATTY 2 cut(s) 867, 1032
AcvI CACGTG 1 cut(s) 428
AcyI GRCGYC 1 cut(s) 973
AfaI GTAC 2 cut(s) 370, 635
AfiI CCNNNNNNNGG 3 cut(s) 58, 356, 513
AgsI TTSAA 2 cut(s) 378, 689
AjnI CCWGG 3 cut(s) 104, 776, 975
AluBI AGCT 6 cut(s) 91, 113, 143, 214, 510, 983
AluI AGCT 6 cut(s) 91, 113, 143, 214, 510, 983
Alw21I GWGCWC 3 cut(s) 145, 748, 1024
Alw26I GTCTC 3 cut(s) 29, 225, 301
Alw44I GTGCAC 2 cut(s) 744, 1020
AlwI GGATC 4 cut(s) 177, 215, 395, 883
ApaLI GTGCAC 2 cut(s) 744, 1020
ApeKI GCWGC 4 cut(s) 113, 211, 599, 980
ApoI RAATTY 2 cut(s) 867, 1032
Asp718I GGTACC 1 cut(s) 368
AspLEI GCGC 1 cut(s) 975
AspS9I GGNCC 2 cut(s) 722, 906
AsuHPI GGTGA 3 cut(s) 371, 898, 968
AvaII GGWCC 2 cut(s) 722, 906
AxyI CCTNAGG 1 cut(s) 44
BaeGI GKGCMC 2 cut(s) 748, 1024
BaeI ACNNNNGTAYC 2 cut(s) 352, 385
BanI GGYRCC 3 cut(s) 48, 368, 972
BanII GRGCYC 3 cut(s) 145, 425, 568
BauI CACGAG 1 cut(s) 447
BbrPI CACGTG 1 cut(s) 428
BbsI GAAGAC 1 cut(s) 661
Bbv12I GWGCWC 3 cut(s) 145, 748, 1024
BbvI GCAGC 4 cut(s) 100, 223, 586, 992
BccI CCATC 2 cut(s) 84, 411
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BciT130I CCWGG 3 cut(s) 106, 778, 977
BcoDI GTCTC 3 cut(s) 29, 225, 301
BfaI CTAG 1 cut(s) 857
BfoI RGCGCY 1 cut(s) 976
BisI GCNGC 4 cut(s) 114, 212, 600, 981
BlsI GCNGC 4 cut(s) 115, 213, 601, 982
Bme1390I CCNGG 3 cut(s) 106, 778, 977
Bme18I GGWCC 2 cut(s) 722, 906
BmgT120I GGNCC 2 cut(s) 722, 906
BmiI GGNNCC 8 cut(s) 50, 63, 370, 422, 567, 723, 907, 974
BmrFI CCNGG 3 cut(s) 106, 778, 977
BmsI GCATC 2 cut(s) 328, 440
BpiI GAAGAC 1 cut(s) 661
BpmI CTGGAG 1 cut(s) 760
Bpu10I CCTNAGC 1 cut(s) 607
BsaAI YACGTR 1 cut(s) 428
BsaHI GRCGYC 1 cut(s) 973
BsaI GGTCTC 2 cut(s) 29, 225
BsaJI CCNNGG 2 cut(s) 364, 763
BsaXI ACNNNNNCTCC 2 cut(s) 412, 442
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 356, 513
Bse1I ACTGG 1 cut(s) 361
Bse21I CCTNAGG 1 cut(s) 44
Bse3DI GCAATG 2 cut(s) 459, 852
BseBI CCWGG 3 cut(s) 106, 778, 977
BseDI CCNNGG 2 cut(s) 364, 763
BseGI GGATG 1 cut(s) 76
BseLI CCNNNNNNNGG 3 cut(s) 58, 356, 513
BseMI GCAATG 2 cut(s) 459, 852
BseMII CTCAG 2 cut(s) 621, 810
BseNI ACTGG 1 cut(s) 361
BseSI GKGCMC 2 cut(s) 748, 1024
BseXI GCAGC 4 cut(s) 100, 223, 586, 992
BseYI CCCAGC 1 cut(s) 851
Bsh1285I CGRYCG 1 cut(s) 951
BshNI GGYRCC 3 cut(s) 48, 368, 972
BsiEI CGRYCG 1 cut(s) 951
BsiHKAI GWGCWC 3 cut(s) 145, 748, 1024
BslFI GGGAC 1 cut(s) 919
BslI CCNNNNNNNGG 3 cut(s) 58, 356, 513
BsmAI GTCTC 3 cut(s) 29, 225, 301
BsmFI GGGAC 1 cut(s) 919
Bso31I GGTCTC 2 cut(s) 29, 225
Bsp1286I GDGCHC 5 cut(s) 145, 425, 568, 748, 1024
Bsp143I GATC 6 cut(s) 169, 220, 400, 577, 888, 948
Bsp19I CCATGG 2 cut(s) 364, 763
BspCNI CTCAG 2 cut(s) 620, 811
BspLI GGNNCC 8 cut(s) 50, 63, 370, 422, 567, 723, 907, 974
BspPI GGATC 4 cut(s) 177, 215, 395, 883
BspT107I GGYRCC 3 cut(s) 48, 368, 972
BspTNI GGTCTC 2 cut(s) 29, 225
BsrDI GCAATG 2 cut(s) 459, 852
BsrI ACTGG 1 cut(s) 361
BssECI CCNNGG 2 cut(s) 364, 763
BssMI GATC 6 cut(s) 169, 220, 400, 577, 888, 948
BssNI GRCGYC 1 cut(s) 973
BssSI CACGAG 1 cut(s) 447
BssT1I CCWWGG 2 cut(s) 364, 763
Bst2BI CACGAG 1 cut(s) 447
Bst2UI CCWGG 3 cut(s) 106, 778, 977
Bst4CI ACNGT 7 cut(s) 295, 315, 661, 697, 733, 996, 1026
BstACI GRCGYC 1 cut(s) 973
BstAPI GCANNNNNTGC 1 cut(s) 458
BstBAI YACGTR 1 cut(s) 428
BstC8I GCNNGC 3 cut(s) 111, 463, 604
BstDEI CTNAG 3 cut(s) 44, 607, 819
BstDSI CCRYGG 2 cut(s) 364, 763
BstEII GGTNACC 1 cut(s) 359
BstF5I GGATG 1 cut(s) 76
BstH2I RGCGCY 1 cut(s) 976
BstHHI GCGC 1 cut(s) 975
BstKTI GATC 6 cut(s) 172, 223, 403, 580, 891, 951
BstMAI GTCTC 3 cut(s) 29, 225, 301
BstMBI GATC 6 cut(s) 169, 220, 400, 577, 888, 948
BstMCI CGRYCG 1 cut(s) 951
BstMWI GCNNNNNNNGC 1 cut(s) 458
BstNI CCWGG 3 cut(s) 106, 778, 977
BstNSI RCATGY 2 cut(s) 465, 606
BstPI GGTNACC 1 cut(s) 359
BstSCI CCNGG 3 cut(s) 104, 776, 975
BstSLI GKGCMC 2 cut(s) 748, 1024
BstV1I GCAGC 4 cut(s) 100, 223, 586, 992
BstV2I GAAGAC 1 cut(s) 661
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
Bsu36I CCTNAGG 1 cut(s) 44
BtgI CCRYGG 2 cut(s) 364, 763
BtgZI GCGATG 1 cut(s) 430
BtsCI GGATG 1 cut(s) 76
BtsI GCAGTG 1 cut(s) 340
BtsIMutI CAGTG 2 cut(s) 291, 340
Cac8I GCNNGC 3 cut(s) 111, 463, 604
CfoI GCGC 1 cut(s) 975
Cfr13I GGNCC 2 cut(s) 722, 906
CseI GACGC 1 cut(s) 41
Csp6I GTAC 2 cut(s) 369, 634
CviAII CATG 7 cut(s) 57, 162, 365, 462, 603, 764, 902
CviQI GTAC 2 cut(s) 369, 634
DdeI CTNAG 3 cut(s) 44, 607, 819
DinI GGCGCC 1 cut(s) 974
DpnI GATC 6 cut(s) 171, 222, 402, 579, 890, 950
DpnII GATC 6 cut(s) 169, 220, 400, 577, 888, 948
Ecl136II GAGCTC 1 cut(s) 143
Eco130I CCWWGG 2 cut(s) 364, 763
Eco24I GRGCYC 3 cut(s) 145, 425, 568
Eco31I GGTCTC 2 cut(s) 29, 225
Eco47I GGWCC 2 cut(s) 722, 906
Eco53kI GAGCTC 1 cut(s) 143
Eco72I CACGTG 1 cut(s) 428
Eco81I CCTNAGG 1 cut(s) 44
Eco91I GGTNACC 1 cut(s) 359
EcoICRI GAGCTC 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 906
EcoO65I GGTNACC 1 cut(s) 359
EcoRII CCWGG 3 cut(s) 104, 776, 975
EcoT14I CCWWGG 2 cut(s) 364, 763
EcoT38I GRGCYC 3 cut(s) 145, 425, 568
EgeI GGCGCC 1 cut(s) 974
EheI GGCGCC 1 cut(s) 974
ErhI CCWWGG 2 cut(s) 364, 763
FaeI CATG 7 cut(s) 60, 165, 368, 465, 606, 767, 905
FalI AAGNNNNNCTT 2 cut(s) 952, 984
FaqI GGGAC 1 cut(s) 919
FatI CATG 7 cut(s) 56, 161, 364, 461, 602, 763, 901
Fnu4HI GCNGC 4 cut(s) 114, 212, 600, 981
FokI GGATG 1 cut(s) 63
FriOI GRGCYC 3 cut(s) 145, 425, 568
Fsp4HI GCNGC 4 cut(s) 114, 212, 600, 981
FspBI CTAG 1 cut(s) 857
GlaI GCGC 1 cut(s) 974
GluI GCNGC 4 cut(s) 114, 212, 600, 981
GsaI CCCAGC 1 cut(s) 855
GsuI CTGGAG 1 cut(s) 760
HaeII RGCGCY 1 cut(s) 976
HgaI GACGC 1 cut(s) 41
HhaI GCGC 1 cut(s) 975
Hin1I GRCGYC 1 cut(s) 973
Hin1II CATG 7 cut(s) 60, 165, 368, 465, 606, 767, 905
Hin6I GCGC 1 cut(s) 973
HinP1I GCGC 1 cut(s) 973
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 1 cut(s) 791
HphI GGTGA 3 cut(s) 371, 898, 968
Hpy166II GTNNAC 3 cut(s) 346, 746, 1022
Hpy188I TCNGA 9 cut(s) 7, 516, 796, 820, 898, 913, 931, 953, 987
Hpy188III TCNNGA 3 cut(s) 230, 575, 946
Hpy8I GTNNAC 3 cut(s) 346, 746, 1022
Hpy99I CGWCG 2 cut(s) 35, 231
HpyAV CCTTC 3 cut(s) 14, 76, 793
HpyCH4III ACNGT 7 cut(s) 295, 315, 661, 697, 733, 996, 1026
HpyCH4IV ACGT 3 cut(s) 427, 636, 933
HpyF10VI GCNNNNNNNGC 1 cut(s) 458
HpyF3I CTNAG 3 cut(s) 44, 607, 819
HpySE526I ACGT 3 cut(s) 427, 636, 933
Hsp92I GRCGYC 1 cut(s) 973
Hsp92II CATG 7 cut(s) 60, 165, 368, 465, 606, 767, 905
HspAI GCGC 1 cut(s) 973
KasI GGCGCC 1 cut(s) 972
KpnI GGTACC 1 cut(s) 372
Kzo9I GATC 6 cut(s) 169, 220, 400, 577, 888, 948
LmnI GCTCC 4 cut(s) 88, 420, 571, 988
Lsp1109I GCAGC 4 cut(s) 100, 223, 586, 992
LweI GCATC 2 cut(s) 328, 440
MaeI CTAG 1 cut(s) 857
MaeII ACGT 3 cut(s) 427, 636, 933
MaeIII GTNAC 1 cut(s) 359
MalI GATC 6 cut(s) 171, 222, 402, 579, 890, 950
MboI GATC 6 cut(s) 169, 220, 400, 577, 888, 948
MboII GAAGA 1 cut(s) 661
MflI RGATCY 1 cut(s) 169
MhlI GDGCHC 5 cut(s) 145, 425, 568, 748, 1024
MluCI AATT 5 cut(s) 202, 408, 570, 867, 1032
Mly113I GGCGCC 1 cut(s) 973
MmeI TCCRAC 3 cut(s) 66, 306, 494
MnlI CCTC 9 cut(s) 160, 234, 271, 379, 456, 694, 902, 919, 947
MseI TTAA 2 cut(s) 503, 738
MspA1I CMGCKG 1 cut(s) 510
MspR9I CCNGG 3 cut(s) 106, 778, 977
MvaI CCWGG 3 cut(s) 106, 778, 977
MwoI GCNNNNNNNGC 1 cut(s) 458
NarI GGCGCC 1 cut(s) 973
NcoI CCATGG 2 cut(s) 364, 763
NdeII GATC 6 cut(s) 169, 220, 400, 577, 888, 948
NlaIII CATG 7 cut(s) 60, 165, 368, 465, 606, 767, 905
NlaIV GGNNCC 8 cut(s) 50, 63, 370, 422, 567, 723, 907, 974
NmuCI GTSAC 1 cut(s) 359
NspI RCATGY 2 cut(s) 465, 606
PaeI GCATGC 2 cut(s) 465, 606
PfeI GAWTC 1 cut(s) 791
PflMI CCANNNNNTGG 1 cut(s) 356
PkrI GCNGC 4 cut(s) 115, 213, 601, 982
Ple19I CGATCG 1 cut(s) 951
PluTI GGCGCC 1 cut(s) 976
PmaCI CACGTG 1 cut(s) 428
PmlI CACGTG 1 cut(s) 428
Ppu21I YACGTR 1 cut(s) 428
PpuMI RGGWCCY 1 cut(s) 906
Psp124BI GAGCTC 1 cut(s) 145
Psp5II RGGWCCY 1 cut(s) 906
Psp6I CCWGG 3 cut(s) 104, 776, 975
PspCI CACGTG 1 cut(s) 428
PspEI GGTNACC 1 cut(s) 359
PspFI CCCAGC 1 cut(s) 851
PspGI CCWGG 3 cut(s) 104, 776, 975
PspN4I GGNNCC 8 cut(s) 50, 63, 370, 422, 567, 723, 907, 974
PspPI GGNCC 2 cut(s) 722, 906
PspPPI RGGWCCY 1 cut(s) 906
PsuI RGATCY 1 cut(s) 169
PvuI CGATCG 1 cut(s) 951
PvuII CAGCTG 1 cut(s) 510
RsaI GTAC 2 cut(s) 370, 635
RsaNI GTAC 2 cut(s) 369, 634
SacI GAGCTC 1 cut(s) 145
SaqAI TTAA 2 cut(s) 503, 738
SatI GCNGC 4 cut(s) 114, 212, 600, 981
Sau3AI GATC 6 cut(s) 169, 220, 400, 577, 888, 948
Sau96I GGNCC 2 cut(s) 722, 906
ScrFI CCNGG 3 cut(s) 106, 778, 977
SduI GDGCHC 5 cut(s) 145, 425, 568, 748, 1024
SfaNI GCATC 2 cut(s) 328, 440
SfoI GGCGCC 1 cut(s) 974
SinI GGWCC 2 cut(s) 722, 906
SphI GCATGC 2 cut(s) 465, 606
Sse9I AATT 5 cut(s) 202, 408, 570, 867, 1032
SspDI GGCGCC 1 cut(s) 972
SspMI CTAG 1 cut(s) 857
SstI GAGCTC 1 cut(s) 145
StyD4I CCNGG 3 cut(s) 104, 776, 975
StyI CCWWGG 2 cut(s) 364, 763
TaaI ACNGT 7 cut(s) 295, 315, 661, 697, 733, 996, 1026
TaiI ACGT 3 cut(s) 430, 639, 936
TaqI TCGA 3 cut(s) 145, 229, 672
TasI AATT 5 cut(s) 202, 408, 570, 867, 1032
TfiI GAWTC 1 cut(s) 791
Tru1I TTAA 2 cut(s) 503, 738
Tru9I TTAA 2 cut(s) 503, 738
TscAI CASTG 2 cut(s) 298, 340
TseFI GTSAC 1 cut(s) 359
TseI GCWGC 4 cut(s) 113, 211, 599, 980
Tsp45I GTSAC 1 cut(s) 359
TspDTI ATGAA 3 cut(s) 110, 207, 879
TspRI CASTG 2 cut(s) 298, 340
Van91I CCANNNNNTGG 1 cut(s) 356
VneI GTGCAC 2 cut(s) 744, 1020
VpaK11BI GGWCC 2 cut(s) 722, 906
XapI RAATTY 2 cut(s) 867, 1032
XceI RCATGY 2 cut(s) 465, 606
XcmI CCANNNNNNNNNTGG 1 cut(s) 204
XspI CTAG 1 cut(s) 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.