Prupe.8G238100_v2.0.a1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0010100
Physical Location & Seq
Forward (+)
20979445 .. 20984025
4581 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G238100.1

Sequence Viewer

Length: 1041 bp
ATGTCTGAACAACTGATAGAAGGTCTCCCTGACGCTGTTTCCCTTAGGTGTCTTGCATGGGTTCCTTTCTATCTCCATCCCAAGTTGGAGCTTGTTTCTCGTTCCTGGCAAGCTGCCATTCGTAGCCCTGAACTTTTTAGAGCTCGACAGGAGGTTGGCTCATGTGAGGATCTACTATGCGTGTCCGCTTTTGACCCAGATAATTTATGGCAGCTATATGACCCTCATCGAGATCTTTGGATTACACTCCCTGTTCTCCCCTCTAAAATCAGAAACCTTTCTCACTTTGGTGCTGTCTCTGCTGCCGGAAAATTGTTTGTTCTGGGTGGTGGCAGTGGCGCTGTTGACCCACAGACTGGCGACCAAGATGGCAGCTTTGCAACCAATGAGGTGTGGTCATATGATCCTGTAATTAGGCGATGGACACCACGTGCATCTATGCTTGTGCCTCGTGCGATGTTTGCATGCTGTGTTTTAGGCGGAAAGATTGTTGTTGCAGGTGGTTTCACCAGCTGTCGAAAATCAATTTCTCAGGCAGAAATGTATGACCCTGAGAAGGATGCGTGGGATCCAATTCCTGATCTCCATCGCACTCATAATTCTGCATGCTCAGGAATAGTGATTGGAGGAAAGGTGCATGTCTTACACAAGGGTTTATCAACGGTGCAAGTCTTGGACAATGTGGGGCATGAGTGGACGGTGGAGGATTATGGTTGGCAACAGGGTCCAATGACAGTTGTTAAGGGTGCACTTTATGTGATGAGCCATGGTGTTATCTCCAAGCAAGATAGAGAATCAGTGAAGGTATTGGTTTCGGCATCTGAGTTTGGTCGCAGAATTGGGTTTGCAATGACTGGACTAGCAGATGAGATTTATGTGATTGGTGGGGTGATTGTCCCTGACCAATGGAACTTGGAAATCAAGCCAATGTCTGATGTTGATGTTCTCACGATTGGGAGTGAAAGACCGACTTGGCGCCAGGCAGCTCCAATGACACGGTGCCGTGGAACGATTCTTGGTTGTACACAGTTGAGAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

347

Amino Acids

38.12

Weight (kDa)

6.15

Isoelectric Point (pI)

45.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 488
Acc36I ACCTGC 1 cut(s) 488
AccB1I GGYRCC 2 cut(s) 975, 999
AccB7I CCANNNNNTGG 1 cut(s) 356
AciI CCGC 2 cut(s) 186, 480
AclWI GGATC 4 cut(s) 177, 398, 563, 576
AcsI RAATTY 1 cut(s) 1035
AcvI CACGTG 1 cut(s) 431
AcyI GRCGYC 1 cut(s) 976
AdeI CACNNNGTG 1 cut(s) 431
AfaI GTAC 1 cut(s) 1024
AfiI CCNNNNNNNGG 2 cut(s) 356, 556
AjnI CCWGG 2 cut(s) 104, 978
AleI CACNNNNGTG 1 cut(s) 288
AluBI AGCT 7 cut(s) 91, 113, 143, 214, 375, 513, 986
AluI AGCT 7 cut(s) 91, 113, 143, 214, 375, 513, 986
Alw21I GWGCWC 2 cut(s) 145, 751
Alw26I GTCTC 2 cut(s) 29, 301
Alw44I GTGCAC 1 cut(s) 747
AlwI GGATC 4 cut(s) 177, 398, 563, 576
ApaLI GTGCAC 1 cut(s) 747
ApeKI GCWGC 5 cut(s) 113, 211, 302, 372, 983
ApoI RAATTY 1 cut(s) 1035
Asp700I GAANNNNTTC 1 cut(s) 277
AspLEI GCGC 2 cut(s) 341, 978
AspS9I GGNCC 1 cut(s) 725
AsuHPI GGTGA 2 cut(s) 499, 901
AvaII GGWCC 1 cut(s) 725
AxyI CCTNAGG 1 cut(s) 44
BaeGI GKGCMC 1 cut(s) 751
BamHI GGATCC 1 cut(s) 568
BanI GGYRCC 2 cut(s) 975, 999
BanII GRGCYC 1 cut(s) 145
BauI CACGAG 1 cut(s) 450
BbrPI CACGTG 1 cut(s) 431
Bbv12I GWGCWC 2 cut(s) 145, 751
BbvI GCAGC 5 cut(s) 100, 223, 289, 384, 995
BccI CCATC 4 cut(s) 84, 362, 414, 594
BceAI ACGGC 1 cut(s) 987
BciT130I CCWGG 2 cut(s) 106, 980
BcoDI GTCTC 2 cut(s) 29, 301
BfaI CTAG 1 cut(s) 860
BfoI RGCGCY 2 cut(s) 342, 979
BfuAI ACCTGC 1 cut(s) 488
BglII AGATCT 1 cut(s) 232
BisI GCNGC 5 cut(s) 114, 212, 303, 373, 984
BlsI GCNGC 5 cut(s) 115, 213, 304, 374, 985
Bme1390I CCNGG 2 cut(s) 106, 980
Bme18I GGWCC 1 cut(s) 725
BmgT120I GGNCC 1 cut(s) 725
BmiI GGNNCC 5 cut(s) 63, 570, 726, 977, 1001
BmrFI CCNGG 2 cut(s) 106, 980
BmsI GCATC 3 cut(s) 443, 550, 827
BplI GAGNNNNNCTC 2 cut(s) 143, 175
Bpu10I CCTNAGC 1 cut(s) 610
BsaAI YACGTR 1 cut(s) 431
BsaBI GATNNNNATC 1 cut(s) 585
BsaHI GRCGYC 1 cut(s) 976
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 766, 1003
BsaXI ACNNNNNCTCC 2 cut(s) 618, 648
Bsc4I CCNNNNNNNGG 2 cut(s) 356, 556
Bse1I ACTGG 2 cut(s) 361, 859
Bse21I CCTNAGG 1 cut(s) 44
Bse3DI GCAATG 1 cut(s) 855
Bse8I GATNNNNATC 1 cut(s) 585
BseBI CCWGG 2 cut(s) 106, 980
BseDI CCNNGG 2 cut(s) 766, 1003
BseGI GGATG 2 cut(s) 76, 565
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 2 cut(s) 356, 556
BseMI GCAATG 1 cut(s) 855
BseMII CTCAG 4 cut(s) 543, 545, 624, 813
BseNI ACTGG 2 cut(s) 361, 859
BseSI GKGCMC 1 cut(s) 751
BseXI GCAGC 5 cut(s) 100, 223, 289, 384, 995
BshNI GGYRCC 2 cut(s) 975, 999
BsiHKAI GWGCWC 2 cut(s) 145, 751
BsiSI CCGG 1 cut(s) 306
BslFI GGGAC 1 cut(s) 881
BslI CCNNNNNNNGG 2 cut(s) 356, 556
BsmAI GTCTC 2 cut(s) 29, 301
BsmFI GGGAC 1 cut(s) 881
Bso31I GGTCTC 1 cut(s) 29
Bsp1286I GDGCHC 2 cut(s) 145, 751
Bsp1407I TGTACA 1 cut(s) 1022
Bsp143I GATC 5 cut(s) 169, 232, 403, 568, 580
Bsp19I CCATGG 1 cut(s) 766
BspACI CCGC 2 cut(s) 186, 480
BspCNI CTCAG 4 cut(s) 544, 544, 623, 814
BspLI GGNNCC 5 cut(s) 63, 570, 726, 977, 1001
BspMI ACCTGC 1 cut(s) 488
BspPI GGATC 4 cut(s) 177, 398, 563, 576
BspT107I GGYRCC 2 cut(s) 975, 999
BspTNI GGTCTC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 855
BsrGI TGTACA 1 cut(s) 1022
BsrI ACTGG 2 cut(s) 361, 859
BssECI CCNNGG 2 cut(s) 766, 1003
BssMI GATC 5 cut(s) 169, 232, 403, 568, 580
BssNI GRCGYC 1 cut(s) 976
BssSI CACGAG 1 cut(s) 450
BssT1I CCWWGG 1 cut(s) 766
Bst2BI CACGAG 1 cut(s) 450
Bst2UI CCWGG 2 cut(s) 106, 980
Bst4CI ACNGT 5 cut(s) 664, 700, 736, 999, 1029
BstACI GRCGYC 1 cut(s) 976
BstAUI TGTACA 1 cut(s) 1022
BstBAI YACGTR 1 cut(s) 431
BstC8I GCNNGC 3 cut(s) 111, 466, 607
BstDEI CTNAG 5 cut(s) 44, 531, 552, 610, 822
BstDSI CCRYGG 2 cut(s) 766, 1003
BstF5I GGATG 2 cut(s) 76, 565
BstH2I RGCGCY 2 cut(s) 342, 979
BstHHI GCGC 2 cut(s) 341, 978
BstKTI GATC 5 cut(s) 172, 235, 406, 571, 583
BstMAI GTCTC 2 cut(s) 29, 301
BstMBI GATC 5 cut(s) 169, 232, 403, 568, 580
BstMWI GCNNNNNNNGC 2 cut(s) 299, 461
BstNI CCWGG 2 cut(s) 106, 980
BstNSI RCATGY 3 cut(s) 468, 609, 641
BstSCI CCNGG 2 cut(s) 104, 978
BstSLI GKGCMC 1 cut(s) 751
BstV1I GCAGC 5 cut(s) 100, 223, 289, 384, 995
BstX2I RGATCY 3 cut(s) 169, 232, 568
BstYI RGATCY 3 cut(s) 169, 232, 568
Bsu36I CCTNAGG 1 cut(s) 44
BtgI CCRYGG 2 cut(s) 766, 1003
BtgZI GCGATG 3 cut(s) 433, 470, 572
BtsCI GGATG 2 cut(s) 76, 565
BtsI GCAGTG 1 cut(s) 340
BtsIMutI CAGTG 2 cut(s) 340, 804
BveI ACCTGC 1 cut(s) 488
Cac8I GCNNGC 3 cut(s) 111, 466, 607
CfoI GCGC 2 cut(s) 341, 978
Cfr13I GGNCC 1 cut(s) 725
CseI GACGC 1 cut(s) 41
Csp6I GTAC 1 cut(s) 1023
CviAII CATG 7 cut(s) 57, 162, 465, 606, 638, 689, 767
CviQI GTAC 1 cut(s) 1023
DdeI CTNAG 5 cut(s) 44, 531, 552, 610, 822
DinI GGCGCC 1 cut(s) 977
DpnI GATC 5 cut(s) 171, 234, 405, 570, 582
DpnII GATC 5 cut(s) 169, 232, 403, 568, 580
DraIII CACNNNGTG 1 cut(s) 431
EciI GGCGGA 1 cut(s) 495
Ecl136II GAGCTC 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 766
Eco24I GRGCYC 1 cut(s) 145
Eco31I GGTCTC 1 cut(s) 29
Eco47I GGWCC 1 cut(s) 725
Eco53kI GAGCTC 1 cut(s) 143
Eco72I CACGTG 1 cut(s) 431
Eco81I CCTNAGG 1 cut(s) 44
EcoICRI GAGCTC 1 cut(s) 143
EcoRII CCWGG 2 cut(s) 104, 978
EcoT14I CCWWGG 1 cut(s) 766
EcoT38I GRGCYC 1 cut(s) 145
EgeI GGCGCC 1 cut(s) 977
EheI GGCGCC 1 cut(s) 977
ErhI CCWWGG 1 cut(s) 766
FaeI CATG 7 cut(s) 60, 165, 468, 609, 641, 692, 770
FalI AAGNNNNNCTT 2 cut(s) 955, 987
FaqI GGGAC 1 cut(s) 881
FatI CATG 7 cut(s) 56, 161, 464, 605, 637, 688, 766
FauNDI CATATG 1 cut(s) 400
Fnu4HI GCNGC 5 cut(s) 114, 212, 303, 373, 984
FokI GGATG 2 cut(s) 63, 572
FriOI GRGCYC 1 cut(s) 145
Fsp4HI GCNGC 5 cut(s) 114, 212, 303, 373, 984
FspBI CTAG 1 cut(s) 860
GlaI GCGC 2 cut(s) 340, 977
GluI GCNGC 5 cut(s) 114, 212, 303, 373, 984
HaeII RGCGCY 2 cut(s) 342, 979
HapII CCGG 1 cut(s) 306
HgaI GACGC 1 cut(s) 41
HhaI GCGC 2 cut(s) 341, 978
Hin1I GRCGYC 1 cut(s) 976
Hin1II CATG 7 cut(s) 60, 165, 468, 609, 641, 692, 770
Hin6I GCGC 2 cut(s) 339, 976
HinP1I GCGC 2 cut(s) 339, 976
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 2 cut(s) 794, 1012
HpaII CCGG 1 cut(s) 306
HphI GGTGA 2 cut(s) 499, 901
Hpy166II GTNNAC 4 cut(s) 346, 696, 749, 1025
Hpy188I TCNGA 4 cut(s) 7, 272, 823, 934
Hpy188III TCNNGA 4 cut(s) 230, 578, 612, 949
Hpy8I GTNNAC 4 cut(s) 346, 696, 749, 1025
HpyAV CCTTC 3 cut(s) 14, 550, 796
HpyCH4III ACNGT 5 cut(s) 664, 700, 736, 999, 1029
HpyCH4IV ACGT 1 cut(s) 430
HpyF10VI GCNNNNNNNGC 2 cut(s) 299, 461
HpyF3I CTNAG 5 cut(s) 44, 531, 552, 610, 822
HpySE526I ACGT 1 cut(s) 430
Hsp92I GRCGYC 1 cut(s) 976
Hsp92II CATG 7 cut(s) 60, 165, 468, 609, 641, 692, 770
HspAI GCGC 2 cut(s) 339, 976
KasI GGCGCC 1 cut(s) 975
Kzo9I GATC 5 cut(s) 169, 232, 403, 568, 580
LmnI GCTCC 2 cut(s) 88, 991
Lsp1109I GCAGC 5 cut(s) 100, 223, 289, 384, 995
LweI GCATC 3 cut(s) 443, 550, 827
MaeI CTAG 1 cut(s) 860
MaeII ACGT 1 cut(s) 430
MalI GATC 5 cut(s) 171, 234, 405, 570, 582
MboI GATC 5 cut(s) 169, 232, 403, 568, 580
MflI RGATCY 3 cut(s) 169, 232, 568
MhlI GDGCHC 2 cut(s) 145, 751
MluCI AATT 8 cut(s) 202, 311, 411, 525, 573, 598, 837, 1035
Mly113I GGCGCC 1 cut(s) 976
MmeI TCCRAC 1 cut(s) 66
MnlI CCTC 8 cut(s) 145, 160, 234, 271, 382, 459, 620, 697
MroXI GAANNNNTTC 1 cut(s) 277
MseI TTAA 1 cut(s) 741
MslI CAYNNNNRTG 1 cut(s) 288
MspA1I CMGCKG 1 cut(s) 513
MspI CCGG 1 cut(s) 306
MspR9I CCNGG 2 cut(s) 106, 980
MvaI CCWGG 2 cut(s) 106, 980
MwoI GCNNNNNNNGC 2 cut(s) 299, 461
NarI GGCGCC 1 cut(s) 976
NcoI CCATGG 1 cut(s) 766
NdeI CATATG 1 cut(s) 400
NdeII GATC 5 cut(s) 169, 232, 403, 568, 580
NlaIII CATG 7 cut(s) 60, 165, 468, 609, 641, 692, 770
NlaIV GGNNCC 5 cut(s) 63, 570, 726, 977, 1001
NspI RCATGY 3 cut(s) 468, 609, 641
OliI CACNNNNGTG 1 cut(s) 288
PaeI GCATGC 2 cut(s) 468, 609
PaqCI CACCTGC 1 cut(s) 488
PdmI GAANNNNTTC 1 cut(s) 277
PfeI GAWTC 2 cut(s) 794, 1012
PflMI CCANNNNNTGG 1 cut(s) 356
PkrI GCNGC 5 cut(s) 115, 213, 304, 374, 985
PluTI GGCGCC 1 cut(s) 979
PmaCI CACGTG 1 cut(s) 431
PmlI CACGTG 1 cut(s) 431
Ppu21I YACGTR 1 cut(s) 431
Psp124BI GAGCTC 1 cut(s) 145
Psp6I CCWGG 2 cut(s) 104, 978
PspCI CACGTG 1 cut(s) 431
PspGI CCWGG 2 cut(s) 104, 978
PspN4I GGNNCC 5 cut(s) 63, 570, 726, 977, 1001
PspPI GGNCC 1 cut(s) 725
PsuI RGATCY 3 cut(s) 169, 232, 568
PvuII CAGCTG 1 cut(s) 513
RsaI GTAC 1 cut(s) 1024
RsaNI GTAC 1 cut(s) 1023
RseI CAYNNNNRTG 1 cut(s) 288
SacI GAGCTC 1 cut(s) 145
SaqAI TTAA 1 cut(s) 741
SatI GCNGC 5 cut(s) 114, 212, 303, 373, 984
Sau3AI GATC 5 cut(s) 169, 232, 403, 568, 580
Sau96I GGNCC 1 cut(s) 725
ScrFI CCNGG 2 cut(s) 106, 980
SduI GDGCHC 2 cut(s) 145, 751
SfaNI GCATC 3 cut(s) 443, 550, 827
SfoI GGCGCC 1 cut(s) 977
SinI GGWCC 1 cut(s) 725
SmiMI CAYNNNNRTG 1 cut(s) 288
SphI GCATGC 2 cut(s) 468, 609
Sse9I AATT 8 cut(s) 202, 311, 411, 525, 573, 598, 837, 1035
SsiI CCGC 2 cut(s) 186, 480
SspDI GGCGCC 1 cut(s) 975
SspMI CTAG 1 cut(s) 860
SstI GAGCTC 1 cut(s) 145
StyD4I CCNGG 2 cut(s) 104, 978
StyI CCWWGG 1 cut(s) 766
TaaI ACNGT 5 cut(s) 664, 700, 736, 999, 1029
TaiI ACGT 1 cut(s) 433
TaqI TCGA 3 cut(s) 145, 229, 517
TaqII GACCGA 1 cut(s) 982
TasI AATT 8 cut(s) 202, 311, 411, 525, 573, 598, 837, 1035
TatI WGTACW 1 cut(s) 1022
TfiI GAWTC 2 cut(s) 794, 1012
Tru1I TTAA 1 cut(s) 741
Tru9I TTAA 1 cut(s) 741
TscAI CASTG 2 cut(s) 340, 804
TseI GCWGC 5 cut(s) 113, 211, 302, 372, 983
TspRI CASTG 2 cut(s) 340, 804
Van91I CCANNNNNTGG 1 cut(s) 356
VneI GTGCAC 1 cut(s) 747
VpaK11BI GGWCC 1 cut(s) 725
XapI RAATTY 1 cut(s) 1035
XceI RCATGY 3 cut(s) 468, 609, 641
XcmI CCANNNNNNNNNTGG 1 cut(s) 204
XmnI GAANNNNTTC 1 cut(s) 277
XspI CTAG 1 cut(s) 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.