MD11G1282500.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010100
Physical Location & Seq
Forward (+)
40093178 .. 40096411
3234 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1282500.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGTCTGAACAACTGATAGAAGGTCTCCCTGACGCTGTTGCCCTTAGGTGCCTTGCATGGGTTCCCTTCTATCTCCACCCCAAGTTGGAGCTTGTTTCTCGTTCCTGGCAAGCTGCCATTCATAGCGCTGAACTTTTTAGAGTTCGACAGGAAGTTGGCTCATGTGAGGATCTATTATGCGTGTCTGCTAATGAACCAGATAATCTATGGCAACTATATGACCCTCGTCGAGACCTTTGGATTACACTTCCTGTTCTCCCCTCTAAAATAAAAAACCTTTCCAACTTCGGCACTGTCTTTACTGCTGGAAAACTGTTTGTTCTTGGTGGTGGCAGTGATGCTGTTGACCCACAGACTGGTGACCATGGTACCTTTTCAACCAGTGAGGTTTGGTCATACGATCCTGTAACCAGGCAATGGACTCCACGTGCATCTATGCTTGTTCCTCGTGCAATGTTTGCATGCTGTGTTTTAGGTGGAAAGATTGTTGTTGCAGGGGGTTTAACCAGCTGCCGGAAATCAATCTCTCTAGCAGAAATGTATGACCCTAACAAAGATGTGTGGGCTCCAATTCCTGATCTGCATTTATCTCATAATTCAGTATGCTCGGGGATAGTGATTGGAGAAAAGATGTATGTCTTACACAGGGGTTTATCAACGGTGCAAGTCTTCGACAATGTTGGGCGTCAATGGACGGTAGAGGATTATGGTTGGCAACAGGGTCCAATGACAGTCATTAAGGATGCACTCTATGTGATGAGCCATGGAGTAATCTCCAGACAAGACAGAGAATCATCAAAGGTATTGGTTTCGGCTTCTGAGTTCAGTGGCAGAGTTGGGTTTGCAATGGCTGGACTAGGAGATGAAATTTATGTGATTGGTGGGGTGATTCTCTCTGAACATGGGACCTCGGAAATCAAGCAAATATCAGACGTTGATGTTCTCACGATCGGAGATGAAAGACCAACTCGGCGCCAGGCTGCTCCAATGACACGGTGCAAAGGAAGCGTTTATGGTTGTGCACAGTTGAGAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

346

Amino Acids

37.96

Weight (kDa)

5.92

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 71 - 213 1.1e-07 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 77 - 210 1.9e-09 Calicin, beta-propeller domain
Beta-prop_FBX42 PF13415 78 - 190 1.5e-06 FBX42, beta-propeller domain
Kelch_1 PF01344 94 - 146 2.7e-07 Kelch motif
Kelch_FKB95 PF25210 126 - 264 3.4e-16 FKB95, Kelch-repeats domain
Kelch_1 PF01344 149 - 194 1.6e-10 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 368
AccB1I GGYRCC 3 cut(s) 48, 368, 972
AccB7I CCANNNNNTGG 2 cut(s) 356, 417
AclWI GGATC 2 cut(s) 177, 395
AcsI RAATTY 2 cut(s) 867, 1032
AcvI CACGTG 1 cut(s) 428
AcyI GRCGYC 2 cut(s) 685, 973
AfaI GTAC 1 cut(s) 370
AfeI AGCGCT 1 cut(s) 127
AfiI CCNNNNNNNGG 5 cut(s) 58, 85, 356, 417, 513
AgsI TTSAA 1 cut(s) 378
AjnI CCWGG 3 cut(s) 104, 410, 975
AluBI AGCT 3 cut(s) 91, 113, 510
AluI AGCT 3 cut(s) 91, 113, 510
Alw21I GWGCWC 1 cut(s) 1024
Alw26I GTCTC 2 cut(s) 29, 225
Alw44I GTGCAC 1 cut(s) 1020
AlwI GGATC 2 cut(s) 177, 395
Ama87I CYCGRG 1 cut(s) 607
Aor51HI AGCGCT 1 cut(s) 127
ApaLI GTGCAC 1 cut(s) 1020
ApeKI GCWGC 3 cut(s) 113, 510, 980
ApoI RAATTY 2 cut(s) 867, 1032
Asp718I GGTACC 1 cut(s) 368
AspLEI GCGC 2 cut(s) 128, 975
AspS9I GGNCC 2 cut(s) 722, 906
AsuHPI GGTGA 2 cut(s) 371, 898
AvaI CYCGRG 1 cut(s) 607
AvaII GGWCC 2 cut(s) 722, 906
AxyI CCTNAGG 1 cut(s) 44
BaeGI GKGCMC 1 cut(s) 1024
BaeI ACNNNNGTAYC 2 cut(s) 352, 385
BanI GGYRCC 3 cut(s) 48, 368, 972
BanII GRGCYC 1 cut(s) 568
BauI CACGAG 1 cut(s) 447
BbrPI CACGTG 1 cut(s) 428
BbsI GAAGAC 1 cut(s) 661
Bbv12I GWGCWC 1 cut(s) 1024
BbvI GCAGC 3 cut(s) 100, 497, 967
BciT130I CCWGG 3 cut(s) 106, 412, 977
BcoDI GTCTC 2 cut(s) 29, 225
BfaI CTAG 2 cut(s) 530, 857
BfoI RGCGCY 2 cut(s) 129, 976
BisI GCNGC 3 cut(s) 114, 511, 981
BlsI GCNGC 3 cut(s) 115, 512, 982
Bme1390I CCNGG 3 cut(s) 106, 412, 977
Bme18I GGWCC 2 cut(s) 722, 906
BmeT110I CYCGRG 1 cut(s) 607
BmgT120I GGNCC 2 cut(s) 722, 906
BmiI GGNNCC 7 cut(s) 50, 63, 370, 567, 723, 907, 974
BmrFI CCNGG 3 cut(s) 106, 412, 977
BmsI GCATC 3 cut(s) 328, 440, 733
BoxI GACNNNNGTC 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 661
BpmI CTGGAG 1 cut(s) 760
BsaAI YACGTR 1 cut(s) 428
BsaHI GRCGYC 2 cut(s) 685, 973
BsaI GGTCTC 2 cut(s) 29, 225
BsaJI CCNNGG 3 cut(s) 364, 763, 909
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 85, 356, 417, 513
Bse1I ACTGG 2 cut(s) 361, 381
Bse21I CCTNAGG 1 cut(s) 44
Bse3DI GCAATG 3 cut(s) 422, 459, 852
BseBI CCWGG 3 cut(s) 106, 412, 977
BseDI CCNNGG 3 cut(s) 364, 763, 909
BseGI GGATG 1 cut(s) 748
BseLI CCNNNNNNNGG 5 cut(s) 58, 85, 356, 417, 513
BseMI GCAATG 3 cut(s) 422, 459, 852
BseMII CTCAG 1 cut(s) 810
BseNI ACTGG 2 cut(s) 361, 381
BseSI GKGCMC 1 cut(s) 1024
BseXI GCAGC 3 cut(s) 100, 497, 967
Bsh1285I CGRYCG 1 cut(s) 951
BshNI GGYRCC 3 cut(s) 48, 368, 972
BsiEI CGRYCG 1 cut(s) 951
BsiHKAI GWGCWC 1 cut(s) 1024
BsiHKCI CYCGRG 1 cut(s) 607
BsiSI CCGG 1 cut(s) 514
BslFI GGGAC 1 cut(s) 919
BslI CCNNNNNNNGG 5 cut(s) 58, 85, 356, 417, 513
BsmAI GTCTC 2 cut(s) 29, 225
BsmFI GGGAC 1 cut(s) 919
Bso31I GGTCTC 2 cut(s) 29, 225
BsoBI CYCGRG 1 cut(s) 607
Bsp1286I GDGCHC 2 cut(s) 568, 1024
Bsp143I GATC 4 cut(s) 169, 400, 577, 948
Bsp19I CCATGG 2 cut(s) 364, 763
BspCNI CTCAG 1 cut(s) 811
BspLI GGNNCC 7 cut(s) 50, 63, 370, 567, 723, 907, 974
BspPI GGATC 2 cut(s) 177, 395
BspT107I GGYRCC 3 cut(s) 48, 368, 972
BspTNI GGTCTC 2 cut(s) 29, 225
BsrDI GCAATG 3 cut(s) 422, 459, 852
BsrI ACTGG 2 cut(s) 361, 381
BssECI CCNNGG 3 cut(s) 364, 763, 909
BssMI GATC 4 cut(s) 169, 400, 577, 948
BssNI GRCGYC 2 cut(s) 685, 973
BssSI CACGAG 1 cut(s) 447
BssT1I CCWWGG 2 cut(s) 364, 763
Bst2BI CACGAG 1 cut(s) 447
Bst2UI CCWGG 3 cut(s) 106, 412, 977
Bst4CI ACNGT 7 cut(s) 295, 315, 661, 697, 733, 996, 1026
BstACI GRCGYC 2 cut(s) 685, 973
BstAPI GCANNNNNTGC 1 cut(s) 458
BstBAI YACGTR 1 cut(s) 428
BstC8I GCNNGC 2 cut(s) 111, 463
BstDEI CTNAG 2 cut(s) 44, 819
BstDSI CCRYGG 2 cut(s) 364, 763
BstEII GGTNACC 1 cut(s) 359
BstF5I GGATG 1 cut(s) 748
BstH2I RGCGCY 2 cut(s) 129, 976
BstHHI GCGC 2 cut(s) 128, 975
BstKTI GATC 4 cut(s) 172, 403, 580, 951
BstMAI GTCTC 2 cut(s) 29, 225
BstMBI GATC 4 cut(s) 169, 400, 577, 948
BstMCI CGRYCG 1 cut(s) 951
BstMWI GCNNNNNNNGC 2 cut(s) 458, 1005
BstNI CCWGG 3 cut(s) 106, 412, 977
BstNSI RCATGY 1 cut(s) 465
BstPAI GACNNNNGTC 1 cut(s) 225
BstPI GGTNACC 1 cut(s) 359
BstSCI CCNGG 3 cut(s) 104, 410, 975
BstSLI GKGCMC 1 cut(s) 1024
BstV1I GCAGC 3 cut(s) 100, 497, 967
BstV2I GAAGAC 1 cut(s) 661
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
Bsu36I CCTNAGG 1 cut(s) 44
BtgI CCRYGG 2 cut(s) 364, 763
BtsCI GGATG 1 cut(s) 748
BtsI GCAGTG 1 cut(s) 340
BtsIMutI CAGTG 4 cut(s) 291, 340, 388, 832
Cac8I GCNNGC 2 cut(s) 111, 463
CfoI GCGC 2 cut(s) 128, 975
Cfr13I GGNCC 2 cut(s) 722, 906
CseI GACGC 2 cut(s) 41, 674
Csp6I GTAC 1 cut(s) 369
CviAII CATG 6 cut(s) 57, 162, 365, 462, 764, 902
CviJI RGCY 9 cut(s) 91, 113, 159, 510, 566, 762, 815, 851, 980
CviKI_1 RGCY 9 cut(s) 91, 113, 159, 510, 566, 762, 815, 851, 980
CviQI GTAC 1 cut(s) 369
DdeI CTNAG 2 cut(s) 44, 819
DinI GGCGCC 1 cut(s) 974
DpnI GATC 4 cut(s) 171, 402, 579, 950
DpnII GATC 4 cut(s) 169, 400, 577, 948
Eco130I CCWWGG 2 cut(s) 364, 763
Eco24I GRGCYC 1 cut(s) 568
Eco31I GGTCTC 2 cut(s) 29, 225
Eco47I GGWCC 2 cut(s) 722, 906
Eco47III AGCGCT 1 cut(s) 127
Eco72I CACGTG 1 cut(s) 428
Eco81I CCTNAGG 1 cut(s) 44
Eco88I CYCGRG 1 cut(s) 607
Eco91I GGTNACC 1 cut(s) 359
EcoO109I RGGNCCY 1 cut(s) 906
EcoO65I GGTNACC 1 cut(s) 359
EcoRII CCWGG 3 cut(s) 104, 410, 975
EcoT14I CCWWGG 2 cut(s) 364, 763
EcoT38I GRGCYC 1 cut(s) 568
EgeI GGCGCC 1 cut(s) 974
EheI GGCGCC 1 cut(s) 974
ErhI CCWWGG 2 cut(s) 364, 763
FaeI CATG 6 cut(s) 60, 165, 368, 465, 767, 905
FaqI GGGAC 1 cut(s) 919
FatI CATG 6 cut(s) 56, 161, 364, 461, 763, 901
Fnu4HI GCNGC 3 cut(s) 114, 511, 981
FokI GGATG 1 cut(s) 755
FriOI GRGCYC 1 cut(s) 568
Fsp4HI GCNGC 3 cut(s) 114, 511, 981
FspBI CTAG 2 cut(s) 530, 857
GlaI GCGC 2 cut(s) 127, 974
GluI GCNGC 3 cut(s) 114, 511, 981
GsuI CTGGAG 1 cut(s) 760
HaeII RGCGCY 2 cut(s) 129, 976
HapII CCGG 1 cut(s) 514
HgaI GACGC 2 cut(s) 41, 674
HhaI GCGC 2 cut(s) 128, 975
Hin1I GRCGYC 2 cut(s) 685, 973
Hin1II CATG 6 cut(s) 60, 165, 368, 465, 767, 905
Hin6I GCGC 2 cut(s) 126, 973
HinP1I GCGC 2 cut(s) 126, 973
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 3 cut(s) 421, 791, 889
HpaII CCGG 1 cut(s) 514
HphI GGTGA 2 cut(s) 371, 898
Hpy166II GTNNAC 2 cut(s) 346, 1022
Hpy188I TCNGA 6 cut(s) 7, 820, 898, 913, 931, 953
Hpy188III TCNNGA 4 cut(s) 230, 575, 777, 946
Hpy8I GTNNAC 2 cut(s) 346, 1022
Hpy99I CGWCG 1 cut(s) 231
HpyAV CCTTC 2 cut(s) 14, 76
HpyCH4III ACNGT 7 cut(s) 295, 315, 661, 697, 733, 996, 1026
HpyCH4IV ACGT 2 cut(s) 427, 933
HpyF10VI GCNNNNNNNGC 2 cut(s) 458, 1005
HpyF3I CTNAG 2 cut(s) 44, 819
HpySE526I ACGT 2 cut(s) 427, 933
Hsp92I GRCGYC 2 cut(s) 685, 973
Hsp92II CATG 6 cut(s) 60, 165, 368, 465, 767, 905
HspAI GCGC 2 cut(s) 126, 973
KasI GGCGCC 1 cut(s) 972
KpnI GGTACC 1 cut(s) 372
Kzo9I GATC 4 cut(s) 169, 400, 577, 948
LmnI GCTCC 3 cut(s) 88, 571, 988
Lsp1109I GCAGC 3 cut(s) 100, 497, 967
LweI GCATC 3 cut(s) 328, 440, 733
MaeI CTAG 2 cut(s) 530, 857
MaeII ACGT 2 cut(s) 427, 933
MaeIII GTNAC 2 cut(s) 359, 406
MalI GATC 4 cut(s) 171, 402, 579, 950
MboI GATC 4 cut(s) 169, 400, 577, 948
MboII GAAGA 1 cut(s) 661
MflI RGATCY 1 cut(s) 169
MhlI GDGCHC 2 cut(s) 568, 1024
MluCI AATT 4 cut(s) 570, 595, 867, 1032
Mly113I GGCGCC 1 cut(s) 973
MlyI GAGTC 1 cut(s) 415
MmeI TCCRAC 2 cut(s) 66, 306
MnlI CCTC 7 cut(s) 160, 234, 271, 379, 456, 694, 919
MseI TTAA 2 cut(s) 503, 738
MspA1I CMGCKG 1 cut(s) 510
MspI CCGG 1 cut(s) 514
MspR9I CCNGG 3 cut(s) 106, 412, 977
MvaI CCWGG 3 cut(s) 106, 412, 977
MwoI GCNNNNNNNGC 2 cut(s) 458, 1005
NarI GGCGCC 1 cut(s) 973
NcoI CCATGG 2 cut(s) 364, 763
NdeII GATC 4 cut(s) 169, 400, 577, 948
NlaIII CATG 6 cut(s) 60, 165, 368, 465, 767, 905
NlaIV GGNNCC 7 cut(s) 50, 63, 370, 567, 723, 907, 974
NmeAIII GCCGAG 1 cut(s) 949
NmuCI GTSAC 1 cut(s) 359
NspI RCATGY 1 cut(s) 465
PaeI GCATGC 1 cut(s) 465
PfeI GAWTC 2 cut(s) 791, 889
PflMI CCANNNNNTGG 2 cut(s) 356, 417
PkrI GCNGC 3 cut(s) 115, 512, 982
Ple19I CGATCG 1 cut(s) 951
PleI GAGTC 1 cut(s) 415
PluTI GGCGCC 1 cut(s) 976
PmaCI CACGTG 1 cut(s) 428
PmlI CACGTG 1 cut(s) 428
PpsI GAGTC 1 cut(s) 415
Ppu21I YACGTR 1 cut(s) 428
PpuMI RGGWCCY 1 cut(s) 906
PshAI GACNNNNGTC 1 cut(s) 225
Psp5II RGGWCCY 1 cut(s) 906
Psp6I CCWGG 3 cut(s) 104, 410, 975
PspCI CACGTG 1 cut(s) 428
PspEI GGTNACC 1 cut(s) 359
PspGI CCWGG 3 cut(s) 104, 410, 975
PspN4I GGNNCC 7 cut(s) 50, 63, 370, 567, 723, 907, 974
PspPI GGNCC 2 cut(s) 722, 906
PspPPI RGGWCCY 1 cut(s) 906
PsuI RGATCY 1 cut(s) 169
PvuI CGATCG 1 cut(s) 951
PvuII CAGCTG 1 cut(s) 510
RsaI GTAC 1 cut(s) 370
RsaNI GTAC 1 cut(s) 369
SaqAI TTAA 2 cut(s) 503, 738
SatI GCNGC 3 cut(s) 114, 511, 981
Sau3AI GATC 4 cut(s) 169, 400, 577, 948
Sau96I GGNCC 2 cut(s) 722, 906
SchI GAGTC 1 cut(s) 415
ScrFI CCNGG 3 cut(s) 106, 412, 977
SduI GDGCHC 2 cut(s) 568, 1024
SfaNI GCATC 3 cut(s) 328, 440, 733
SfoI GGCGCC 1 cut(s) 974
SinI GGWCC 2 cut(s) 722, 906
SphI GCATGC 1 cut(s) 465
Sse9I AATT 4 cut(s) 570, 595, 867, 1032
SspDI GGCGCC 1 cut(s) 972
SspMI CTAG 2 cut(s) 530, 857
StyD4I CCNGG 3 cut(s) 104, 410, 975
StyI CCWWGG 2 cut(s) 364, 763
TaaI ACNGT 7 cut(s) 295, 315, 661, 697, 733, 996, 1026
TaiI ACGT 2 cut(s) 430, 936
TaqI TCGA 3 cut(s) 145, 229, 672
TasI AATT 4 cut(s) 570, 595, 867, 1032
TfiI GAWTC 2 cut(s) 791, 889
Tru1I TTAA 2 cut(s) 503, 738
Tru9I TTAA 2 cut(s) 503, 738
TscAI CASTG 4 cut(s) 298, 340, 388, 832
TseFI GTSAC 1 cut(s) 359
TseI GCWGC 3 cut(s) 113, 510, 980
Tsp45I GTSAC 1 cut(s) 359
TspDTI ATGAA 4 cut(s) 110, 207, 879, 972
TspRI CASTG 4 cut(s) 298, 340, 388, 832
Van91I CCANNNNNTGG 2 cut(s) 356, 417
VneI GTGCAC 1 cut(s) 1020
VpaK11BI GGWCC 2 cut(s) 722, 906
XapI RAATTY 2 cut(s) 867, 1032
XceI RCATGY 1 cut(s) 465
XcmI CCANNNNNNNNNTGG 1 cut(s) 204
XspI CTAG 2 cut(s) 530, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.