Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0016265

Physical Location & Seq

Genomic Coordinates Forward (+)

35166719 .. 35169030

Gene Structure 2312 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1266500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1272 bp

ATGGGTTCGATCCTTGTACTAAAAAATATTCAAGACAAAGATGAGAGGCACAAGTTTTCAATTTTTGGAATTATTTCCAATCTTAAAAATAGAATAATTGATCAGATAAGCAAGCAAGATATGAGAAAGCTGCTTCACAGCATCAAGGTTGGAGTTGCCCTGGTTCTGGTTTCACTTCTCTATCTTCTCGACCCTCTGTATGAGCAAGTTGGAGAGAATGCCATGTGGGCTATCATGACCGTCGTCGTCATCTTCGAGTTCTACGCAGGTGCCACACTTGGTAAGGGCTTAAACCGTGGATTGGGAACAATTTTAGGAGGTGCTTTGGGGTGCTCAGCTGCAACTTTTGCTCAAAAAGTTGGTGGAATGGGCAAAGGCCACGCTGTATTCATCGGTTCTTCGGTGTTTATCATCGGTGCATTTGCGACATATTTTAGATTAGTACCAAGCATTAAGAAAAGATACGACTATGGAGCCATGATCTTCATCTTGACCTTCAATCTAGTAATCGTGTCGGGTTTACGTGCCGAAAAAGTGTTGGAATTAGCTCGTGATCGTCTTTCAACGATCGGGATGGGATTTGCTGTTTGCATATTCATAAACTTGCTGGTCTTCCCCGCATGGGCTAGTGATGAACTTCATGACTCTACAACTCATAAATTTCAACTCCTTGCGGACTCAATTGAAGGATGCTTGGAGAATTACTTCAGACAGGACAATGAAAATATGAAGGATAACCAGACTTCCATTTCTAGTAGTTGCAAGGCGGTGTTGCACTCCAAGACAAAGGATGAGACACTGGCAAATTTTGCGAAATGGGAACCATGGCATGGAAAGTTTGGTCTCCACTACCCATGGACTAAATACCTACAACTAGGAGAGCTTCTTCGAGAGCTTGCTACCATTGTCATTTCCCTGAAAGGCTGTATTCAATCTCCTAAACAGCCTTCATCGAGTCTGAAGCAGTCAATCGAAGAGCCAAGTGAAGCTGTTGGGGTGACACTTGTATGGAGCCTAAGAGAGCTTGGAGAGAGCCTTGGGAAGATGAGACGATGCAACCAACAGGTTGTGATAGTGCCCAAATTGAAGTCAACGAGACAAGAGCTAAGCTCGATTGTTTCTCCAACTTCCAAATTTGGACCAATAGAAAATGCTGATGAACTTGCAATTGCAAGCTTTGTCTTCTTGTTGACAGAGATAGTTGAAAAGGTGGAAGAATTGGCAAAGGAGATTGAAGAACTTGGAGAGATTGCTGGTTTTCATACTAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0005215 GO:0005310 GO:0005342 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006820 GO:0006835 GO:0008150 GO:0008509 GO:0008514 GO:0009705 GO:0015075 GO:0015140 GO:0015318 GO:0015556 GO:0015711 GO:0015740 GO:0015743 GO:0015849 GO:0016020 GO:0022857 GO:0031090 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071423 GO:0071702 GO:0098588 GO:0098656 GO:0098805 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ALMT

Protein Analysis

424

Amino Acids

46.88

Weight (kDa)

8.09

Isoelectric Point (pI)

39.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ALMT	PF11744	36 - 373	9.2e-121	Aluminium activated malate transporter
FUSC_2	PF13515	66 - 200	1.1e-10	Fusaric acid resistance protein-like
FUSC	PF04632	72 - 236	9.9e-10	Fusaric acid resistance protein family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016265)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g23710
malus_domestica	MD03G1266500.v1.1 MD11G1287000.v1.1 MD11G1287100.v1.1
prunus_persica	Prupe.8G242400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0291181
rosa_laevigata	RLG00000012135
rosa_multiflora	Rmu_sc0013203.1_g000001
rosa_roxburghii	Rroxscaffold_7G00176320
rosa_rugosa	Rorug06G0218300
rosa_samantha	Rh6AG328700 Rh6BG336200 Rh6CG342400 Rh6DG329600
rosa_wichuraiana	Rw6G028590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	257
Acc36I	ACCTGC	1 cut(s)	257
AccB1I	GGYRCC	1 cut(s)	269
AccB7I	CCANNNNNTGG	1 cut(s)	830
AciI	CCGC	3 cut(s)	618, 674, 767
AclWI	GGATC	1 cut(s)	4
AcsI	RAATTY	3 cut(s)	659, 805, 1133
AcuI	CTGAAG	2 cut(s)	691, 980
AfaI	GTAC	2 cut(s)	18, 444
AfiI	CCNNNNNNNGG	4 cut(s)	166, 301, 622, 830
AjnI	CCWGG	1 cut(s)	159
Alw21I	GWGCWC	1 cut(s)	335
Alw26I	GTCTC	4 cut(s)	788, 848, 1042, 1090
AlwI	GGATC	1 cut(s)	4
AoxI	GGCC	1 cut(s)	376
ApeKI	GCWGC	2 cut(s)	130, 338
ApoI	RAATTY	3 cut(s)	659, 805, 1133
Asp700I	GAANNNNTTC	2 cut(s)	73, 704
AspS9I	GGNCC	1 cut(s)	1139
AsuHPI	GGTGA	1 cut(s)	1009
AvaII	GGWCC	1 cut(s)	1139
BaeGI	GKGCMC	1 cut(s)	1080
BanI	GGYRCC	1 cut(s)	269
BauI	CACGAG	1 cut(s)	549
BbsI	GAAGAC	2 cut(s)	604, 1174
Bbv12I	GWGCWC	1 cut(s)	335
BbvI	GCAGC	2 cut(s)	117, 325
BccI	CCATC	1 cut(s)	568
BciT130I	CCWGG	1 cut(s)	161
BclI	TGATCA	1 cut(s)	100
BcoDI	GTCTC	4 cut(s)	788, 848, 1042, 1090
BfaI	CTAG	4 cut(s)	503, 627, 753, 875
BfuAI	ACCTGC	1 cut(s)	257
BglI	GCCNNNNNGGC	1 cut(s)	227
BisI	GCNGC	2 cut(s)	131, 339
BlpI	GCTNAGC	2 cut(s)	334, 1106
BlsI	GCNGC	2 cut(s)	132, 340
Bme1390I	CCNGG	1 cut(s)	161
Bme18I	GGWCC	1 cut(s)	1139
BmgT120I	GGNCC	1 cut(s)	1139
BmiI	GGNNCC	4 cut(s)	271, 475, 822, 1013
BmrFI	CCNGG	1 cut(s)	161
BmsI	GCATC	3 cut(s)	150, 680, 1043
BoxI	GACNNNNGTC	1 cut(s)	242
BpiI	GAAGAC	2 cut(s)	604, 1174
BplI	GAGNNNNNCTC	2 cut(s)	1094, 1126
Bpu1102I	GCTNAGC	2 cut(s)	334, 1106
BsaAI	YACGTR	1 cut(s)	524
BsaBI	GATNNNNATC	1 cut(s)	485
BsaI	GGTCTC	1 cut(s)	848
BsaJI	CCNNGG	5 cut(s)	159, 295, 824, 854, 1036
Bsc4I	CCNNNNNNNGG	4 cut(s)	166, 301, 622, 830
Bse1I	ACTGG	1 cut(s)	804
Bse8I	GATNNNNATC	1 cut(s)	485
BseBI	CCWGG	1 cut(s)	161
BseDI	CCNNGG	5 cut(s)	159, 295, 824, 854, 1036
BseGI	GGATG	3 cut(s)	579, 695, 796
BseJI	GATNNNNATC	1 cut(s)	485
BseLI	CCNNNNNNNGG	4 cut(s)	166, 301, 622, 830
BseMII	CTCAG	1 cut(s)	348
BseNI	ACTGG	1 cut(s)	804
BseSI	GKGCMC	1 cut(s)	1080
BseXI	GCAGC	2 cut(s)	117, 325
Bsh1285I	CGRYCG	1 cut(s)	570
BshFI	GGCC	1 cut(s)	378
BshNI	GGYRCC	1 cut(s)	269
BsiEI	CGRYCG	1 cut(s)	570
BsiHKAI	GWGCWC	1 cut(s)	335
BslI	CCNNNNNNNGG	4 cut(s)	166, 301, 622, 830
BsmAI	GTCTC	4 cut(s)	788, 848, 1042, 1090
BsmBI	CGTCTC	1 cut(s)	1042
BsmI	GAATGC	1 cut(s)	223
BsnI	GGCC	1 cut(s)	378
Bso31I	GGTCTC	1 cut(s)	848
Bsp1286I	GDGCHC	2 cut(s)	335, 1080
Bsp143I	GATC	5 cut(s)	9, 100, 480, 553, 567
Bsp1720I	GCTNAGC	2 cut(s)	334, 1106
Bsp19I	CCATGG	2 cut(s)	824, 854
BspACI	CCGC	3 cut(s)	618, 674, 767
BspANI	GGCC	1 cut(s)	378
BspCNI	CTCAG	1 cut(s)	347
BspHI	TCATGA	2 cut(s)	234, 640
BspLI	GGNNCC	4 cut(s)	271, 475, 822, 1013
BspMI	ACCTGC	1 cut(s)	257
BspPI	GGATC	1 cut(s)	4
BspQI	GCTCTTC	1 cut(s)	969
BspT107I	GGYRCC	1 cut(s)	269
BspTNI	GGTCTC	1 cut(s)	848
BsrI	ACTGG	1 cut(s)	804
BssECI	CCNNGG	5 cut(s)	159, 295, 824, 854, 1036
BssMI	GATC	5 cut(s)	9, 100, 480, 553, 567
BssSI	CACGAG	1 cut(s)	549
BssT1I	CCWWGG	3 cut(s)	824, 854, 1036
Bst2BI	CACGAG	1 cut(s)	549
Bst2UI	CCWGG	1 cut(s)	161
Bst4CI	ACNGT	2 cut(s)	241, 296
Bst6I	CTCTTC	1 cut(s)	969
BstAPI	GCANNNNNTGC	2 cut(s)	347, 809
BstBAI	YACGTR	1 cut(s)	524
BstC8I	GCNNGC	3 cut(s)	113, 897, 1174
BstDEI	CTNAG	3 cut(s)	334, 1016, 1106
BstDSI	CCRYGG	3 cut(s)	295, 824, 854
BstF5I	GGATG	3 cut(s)	579, 695, 796
BstKTI	GATC	5 cut(s)	12, 103, 483, 556, 570
BstMAI	GTCTC	4 cut(s)	788, 848, 1042, 1090
BstMBI	GATC	5 cut(s)	9, 100, 480, 553, 567
BstMCI	CGRYCG	1 cut(s)	570
BstMWI	GCNNNNNNNGC	3 cut(s)	227, 347, 809
BstNI	CCWGG	1 cut(s)	161
BstPAI	GACNNNNGTC	1 cut(s)	242
BstSCI	CCNGG	1 cut(s)	159
BstSLI	GKGCMC	1 cut(s)	1080
BstV1I	GCAGC	2 cut(s)	117, 325
BstV2I	GAAGAC	2 cut(s)	604, 1174
BsuRI	GGCC	1 cut(s)	378
BtgI	CCRYGG	3 cut(s)	295, 824, 854
BtsCI	GGATG	3 cut(s)	579, 695, 796
BtsIMutI	CAGTG	1 cut(s)	797
BveI	ACCTGC	1 cut(s)	257
Cac8I	GCNNGC	3 cut(s)	113, 897, 1174
CciI	TCATGA	2 cut(s)	234, 640
Cfr13I	GGNCC	1 cut(s)	1139
Csp6I	GTAC	2 cut(s)	17, 443
CviAII	CATG	8 cut(s)	223, 235, 478, 621, 641, 825, 830, 855
CviQI	GTAC	2 cut(s)	17, 443
DdeI	CTNAG	3 cut(s)	334, 1016, 1106
DpnI	GATC	5 cut(s)	11, 102, 482, 555, 569
DpnII	GATC	5 cut(s)	9, 100, 480, 553, 567
Eam1104I	CTCTTC	1 cut(s)	969
EarI	CTCTTC	1 cut(s)	969
Eco130I	CCWWGG	3 cut(s)	824, 854, 1036
Eco31I	GGTCTC	1 cut(s)	848
Eco47I	GGWCC	1 cut(s)	1139
Eco57I	CTGAAG	2 cut(s)	691, 980
EcoRII	CCWGG	1 cut(s)	159
EcoT14I	CCWWGG	3 cut(s)	824, 854, 1036
ErhI	CCWWGG	3 cut(s)	824, 854, 1036
Esp3I	CGTCTC	1 cut(s)	1042
FaeI	CATG	8 cut(s)	226, 238, 481, 624, 644, 828, 833, 858
FatI	CATG	8 cut(s)	222, 234, 477, 620, 640, 824, 829, 854
FauI	CCCGC	1 cut(s)	625
FbaI	TGATCA	1 cut(s)	100
Fnu4HI	GCNGC	2 cut(s)	131, 339
FokI	GGATG	3 cut(s)	586, 702, 803
Fsp4HI	GCNGC	2 cut(s)	131, 339
FspBI	CTAG	4 cut(s)	503, 627, 753, 875
GluI	GCNGC	2 cut(s)	131, 339
HaeIII	GGCC	1 cut(s)	378
Hin1II	CATG	8 cut(s)	226, 238, 481, 624, 644, 828, 833, 858
HincII	GTYRAC	2 cut(s)	1092, 1191
HindII	GTYRAC	2 cut(s)	1092, 1191
HindIII	AAGCTT	1 cut(s)	1174
HinfI	GANTC	3 cut(s)	644, 677, 955
HphI	GGTGA	1 cut(s)	1009
Hpy166II	GTNNAC	3 cut(s)	521, 1092, 1191
Hpy188I	TCNGA	3 cut(s)	105, 710, 960
Hpy188III	TCNNGA	8 cut(s)	32, 188, 235, 490, 551, 571, 641, 890
Hpy8I	GTNNAC	3 cut(s)	521, 1092, 1191
Hpy99I	CGWCG	2 cut(s)	245, 248
HpyAV	CCTTC	4 cut(s)	505, 680, 724, 957
HpyCH4III	ACNGT	2 cut(s)	241, 296
HpyCH4IV	ACGT	1 cut(s)	523
HpyCH4V	TGCA	8 cut(s)	341, 419, 591, 762, 775, 1056, 1166, 1172
HpyF10VI	GCNNNNNNNGC	3 cut(s)	227, 347, 809
HpyF3I	CTNAG	3 cut(s)	334, 1016, 1106
HpySE526I	ACGT	1 cut(s)	523
Hsp92II	CATG	8 cut(s)	226, 238, 481, 624, 644, 828, 833, 858
Ksp22I	TGATCA	1 cut(s)	100
Kzo9I	GATC	5 cut(s)	9, 100, 480, 553, 567
LguI	GCTCTTC	1 cut(s)	969
LmnI	GCTCC	2 cut(s)	473, 1011
Lsp1109I	GCAGC	2 cut(s)	117, 325
LweI	GCATC	3 cut(s)	150, 680, 1043
MaeI	CTAG	4 cut(s)	503, 627, 753, 875
MaeII	ACGT	1 cut(s)	523
MaeIII	GTNAC	1 cut(s)	997
MalI	GATC	5 cut(s)	11, 102, 482, 555, 569
MboI	GATC	5 cut(s)	9, 100, 480, 553, 567
MfeI	CAATTG	2 cut(s)	681, 1167
MhlI	GDGCHC	2 cut(s)	335, 1080
MlyI	GAGTC	3 cut(s)	638, 671, 964
MmeI	TCCRAC	4 cut(s)	130, 190, 519, 1148
MnlI	CCTC	3 cut(s)	39, 204, 311
MroXI	GAANNNNTTC	2 cut(s)	73, 704
MseI	TTAA	3 cut(s)	84, 290, 453
MslI	CAYNNNNRTG	1 cut(s)	1006
MspA1I	CMGCKG	1 cut(s)	338
MspR9I	CCNGG	1 cut(s)	161
MunI	CAATTG	2 cut(s)	681, 1167
Mva1269I	GAATGC	1 cut(s)	223
MvaI	CCWGG	1 cut(s)	161
MwoI	GCNNNNNNNGC	3 cut(s)	227, 347, 809
NcoI	CCATGG	2 cut(s)	824, 854
NdeII	GATC	5 cut(s)	9, 100, 480, 553, 567
NlaIII	CATG	8 cut(s)	226, 238, 481, 624, 644, 828, 833, 858
NlaIV	GGNNCC	4 cut(s)	271, 475, 822, 1013
NmuCI	GTSAC	1 cut(s)	997
PagI	TCATGA	2 cut(s)	234, 640
PaqCI	CACCTGC	1 cut(s)	257
PciSI	GCTCTTC	1 cut(s)	969
PcsI	WCGNNNNNNNCGW	1 cut(s)	252
PctI	GAATGC	1 cut(s)	223
PdmI	GAANNNNTTC	2 cut(s)	73, 704
PflMI	CCANNNNNTGG	1 cut(s)	830
PkrI	GCNGC	2 cut(s)	132, 340
Ple19I	CGATCG	1 cut(s)	570
PleI	GAGTC	3 cut(s)	638, 671, 963
PpsI	GAGTC	3 cut(s)	638, 671, 963
Ppu21I	YACGTR	1 cut(s)	524
PshAI	GACNNNNGTC	1 cut(s)	242
Psp6I	CCWGG	1 cut(s)	159
PspGI	CCWGG	1 cut(s)	159
PspN4I	GGNNCC	4 cut(s)	271, 475, 822, 1013
PspPI	GGNCC	1 cut(s)	1139
PvuI	CGATCG	1 cut(s)	570
PvuII	CAGCTG	1 cut(s)	338
RsaI	GTAC	2 cut(s)	18, 444
RsaNI	GTAC	2 cut(s)	17, 443
RseI	CAYNNNNRTG	1 cut(s)	1006
SapI	GCTCTTC	1 cut(s)	969
SaqAI	TTAA	3 cut(s)	84, 290, 453
SatI	GCNGC	2 cut(s)	131, 339
Sau3AI	GATC	5 cut(s)	9, 100, 480, 553, 567
Sau96I	GGNCC	1 cut(s)	1139
SchI	GAGTC	3 cut(s)	638, 671, 964
ScrFI	CCNGG	1 cut(s)	161
SduI	GDGCHC	2 cut(s)	335, 1080
SfaNI	GCATC	3 cut(s)	150, 680, 1043
SinI	GGWCC	1 cut(s)	1139
SmiMI	CAYNNNNRTG	1 cut(s)	1006
SsiI	CCGC	3 cut(s)	618, 674, 767
SspI	AATATT	1 cut(s)	28
SspMI	CTAG	4 cut(s)	503, 627, 753, 875
StyD4I	CCNGG	1 cut(s)	159
StyI	CCWWGG	3 cut(s)	824, 854, 1036
TaaI	ACNGT	2 cut(s)	241, 296
TaiI	ACGT	1 cut(s)	526
TaqI	TCGA	7 cut(s)	8, 189, 255, 889, 953, 972, 1112
TatI	WGTACW	1 cut(s)	16
Tru1I	TTAA	3 cut(s)	84, 290, 453
Tru9I	TTAA	3 cut(s)	84, 290, 453
TscAI	CASTG	1 cut(s)	804
TseFI	GTSAC	1 cut(s)	997
TseI	GCWGC	2 cut(s)	130, 338
Tsp45I	GTSAC	1 cut(s)	997
TspRI	CASTG	1 cut(s)	804
Van91I	CCANNNNNTGG	1 cut(s)	830
VpaK11BI	GGWCC	1 cut(s)	1139
XapI	RAATTY	3 cut(s)	659, 805, 1133
XmnI	GAANNNNTTC	2 cut(s)	73, 704
XspI	CTAG	4 cut(s)	503, 627, 753, 875

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.