Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0013348

Physical Location & Seq

Genomic Coordinates Forward (+)

35349436 .. 35352662

Gene Structure 3227 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1268800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2613 bp

ATGGCAACTCTTCCGCTGTCAATCGCAATACAGGCCTCCTGTTGTTTGAATTGTGGAACCACTGAATTGCCTAAGCTGACAGCTACGGATCGTTATGGACACAGGAACAACGGTGTTCATGGTTATGTGAAATTGGGTACAGAAAGAAATCTAGTTTTACGAGAAGTTGTGCAAATTTTGAAGGAAAGGCCTCTAAGGGACCGTGAATGGAAAGTGTATGCTACATCCAGAGTTTCTGTTGAGCCAGTGGAGCAAAGAGTCTCCACCGGTACTGAAACTGAAGAAGTAGGCAAGGTATCAACTTATCTATTTAGGACAGACACTGGGGATCCGGTAAATGTCTTTGTTAGAAAGAGAAATGCCAAATACGCTGTGAATATCGAAGTTTCATTGTTGCACCTGTCCAGAAGTGATAGGAGACTAGTACTGAGTTGGGGAATGTATAGAGCTGATTCATCTAGTTTCGTGCCTTCGAATTTCAGAAGTTCGACCCCAGCTGACAGAACTACTGCCCTTGAAACTCCATTCACTGGGACTTCTTCTGGCAAGTTTACTCTCGAATTGGAGTTTGAGGCTAAACAAATCCCCTTCTACCTCTCGTTTGTTCTGAAATCTCCCGCAGACGCTGATTTGAGTGACTTGGAAATAAGAAGCCATAGGAAGACAAATTTCTGTTTTCCAGTTGGTTTTGGTCGAGGCTGTCCTGCTCCATTGGGTGTCTCTTTCTCTAATGACGGTTCTATGAACTTTGCCATCTTTTCAAGAAATGCAGAAAGTGTAGTTTTATGCTTGTATGCTGAGACCACAGCTGAAAAACCTGTGTTGGAGCTCGATCTAGATCCATATGTCAATCGATCAGGTGACATCTGGCATGCGTCATTTGAAAATGCATGGGATTTTGTGAGCTATGGTTATAGATTTGATGAAGGGAATGTCTTGCTGGATCCATATGCTAAGATCATTGCGAAATCAGTTCCACATGGGACAGGGTCGAAGTATCTTGGAAGACTATGTGAGGAACCTGCTTTTGAGTGGGCTGGTGATGTTCGGCCTGACTTAACGCTGGAAAAACTAGTGGTTTATCGGTTGAATGTGAAACGTTTCACCAAACACAAGTCTAGTAAGCTACCAACTAACATAGCAGGCACATTTTCGGGTTTGACAGAGAAGTTGGAGCATTTTAAAGCTCTTGGTGTGAATGCAGTTTTGTTAGAGCCGATTTTCCCATTTGATGAGCAAAAGGGTCCATATTTTCCTGTCCATTTCTTTTCACCAATGAATTGTTTCGGACCTCCTAGAGGGCCTGTATCGGCTGTCATTTCTATGAAAGAGATGGTGAAAATTTTTCATGCCGACGGAATAGAGGTCCTACTGGAAGTTGTTTTCACCCACACCTCTGAGGGTGAAGCTATGCAAGGAATTGATATCTCATCGTATTATCACGTAAACGGGGTTGAGGATTTGGAAGCCAGAAATGCTTTGAATTGTAACTATCCTGTTGTGCAACAAATGGTATTGGATAGTCTTAAGTACTGGGTGACTGAGTTTCACGTTGATGGCTTTTGTTTTATAAATGCATCATCTCTGTTGCGAGGGTCTAATGGTGAATACTTATCTCGTCCGCCTTTGGTCGAAGCAATTGCTTTTGATCCTCTACTCTCGAAGACCAAGATCATCGCAGACTGTTGGGATCCCCGTGGCTTGGTACCAAAGGAAACTCGCTTTCCTCACTGGAAGAGGTGGGCAGAAGTGAATACGAAATTCTCTAATGATGTGAGAAACTTTCTGAGGGGTGAGGGTCTTCTCAGTGACCTTGCTACACGGCTTTGTGGGAACGGGGACATCGTCTCAGATGGACGAGGGCCAGCATCCTCTTTCAACTTTATTTCCAGGAACTCCGGACTTTCTCTCGTGGACCTCGTTAGTTTCAGTGGTGTTGAGCTAGCTGCAGAACTGAGTTGCAATTGTGGGGAAGAAGGGCCTACAAATAAAACCGCTGTACTTGAAAGGCGACTTAAACAAATTCGGAACTTTCTCTTTATCTTGTTTGTTTCACTCGGCGTTCCTGTTCTTAACATGGGAGATGAGTACGGACAATCTGCAGGTGGTTCCCCTGCATACAGCGACAGAAAAGCTTTCGACTGGAACGCACTGGGAACAGGTTTTGCTACTCAAACCACACAATTCATCGCATTTTTAAGTTCGTTTAGGATAAGGCGGAGCGACCGACTTCAGAAGAGGAACTTCTTGAAAGAAGAAAATATAGACTGGTACGGGAGTGGTCAATCTTCTCCCAAATGGGAAGATCCATCCTGCAAATTCCTTGCCATGAAGTTGAAAGCAGACAAAGAGGAAGCGTACGAATCAGGAGACGATTCTTCCCCTTCATGGGGTGACTTATTTGTTGCCTTCAGTGCAGCTGATCTATCAGAAACTGTTATTCTGCCTCCACTGCCGGAAGGTATGGGATGGTTCCATTTGGTCGACACGGCTCTTCCATTTCCAGGTTTTTTCTCAACTGACGGCGAGCCAGTTGCTGAACAACCGGCGGGTTTATTTGCGTACGAAATGAAGTCTCACAGTTGTGCTCTGTTTGAAGCCAGAAGCTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009251 GO:0009507 GO:0009536 GO:0009987 GO:0010021 GO:0015980 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0032991 GO:0043033 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0071704 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1902494 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

871

Amino Acids

96.9

Weight (kDa)

5.68

Isoelectric Point (pI)

40.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	237 - 318	1.4e-11	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	376 - 468	5.3e-08	Alpha amylase, catalytic domain
ISOA1-3_C	PF21156	756 - 842	2.1e-06	Isoamylase 1-3, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013348)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03310 AT1G03310
fragaria_vesca	FvH4_2g23950
malus_domestica	MD03G1268800.v1.1 MD11G1289600.v1.1
prunus_persica	Prupe.8G244800_v2.0.a1
pyrus_communis	pycom11g25700
rosa_chinensis	RchiOBHm_Chr6g0291581
rosa_laevigata	RLG00000012105
rosa_multiflora	Rmu_sc0002226.1_g000007
rosa_roxburghii	Rroxscaffold_7G00175950
rosa_rugosa	Rorug06G0220400 Rorug06G0220400
rosa_samantha	Rh6AG332800 Rh6BG339600 Rh6CG346000 Rh6DG333200
rosa_wichuraiana	Rw6G028900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1571
AarI	CACCTGC	1 cut(s)	2093
Acc36I	ACCTGC	2 cut(s)	1030, 2093
Acc65I	GGTACC	1 cut(s)	1705
AccB1I	GGYRCC	1 cut(s)	1705
AccB7I	CCANNNNNTGG	1 cut(s)	530
AccI	GTMKAC	1 cut(s)	2484
AccIII	TCCGGA	1 cut(s)	1898
AciI	CCGC	6 cut(s)	14, 618, 1622, 1995, 2218, 2549
AclI	AACGTT	1 cut(s)	1099
AcsI	RAATTY	7 cut(s)	174, 475, 667, 1341, 1760, 2022, 2318
AcuI	CTGAAG	3 cut(s)	300, 2216, 2395
AfiI	CCNNNNNNNGG	7 cut(s)	530, 1298, 1702, 2388, 2389, 2390, 2504
AflII	CTTAAG	1 cut(s)	1526
AgeI	ACCGGT	1 cut(s)	266
AhlI	ACTAGT	2 cut(s)	421, 1072
AjnI	CCWGG	2 cut(s)	1889, 2503
AleI	CACNNNNGTG	1 cut(s)	2583
Alw21I	GWGCWC	2 cut(s)	831, 2590
Alw26I	GTCTC	7 cut(s)	265, 412, 724, 794, 1852, 2364, 2580
AlwNI	CAGNNNCTG	4 cut(s)	323, 626, 2435, 2537
Aor13HI	TCCGGA	1 cut(s)	1898
AoxI	GGCC	6 cut(s)	33, 188, 1049, 1301, 1862, 1979
ApeKI	GCWGC	2 cut(s)	1946, 2417
ApoI	RAATTY	7 cut(s)	174, 475, 667, 1341, 1760, 2022, 2318
AsiGI	ACCGGT	1 cut(s)	266
Asp700I	GAANNNNTTC	2 cut(s)	1100, 1283
Asp718I	GGTACC	1 cut(s)	1705
AspS9I	GGNCC	8 cut(s)	199, 1244, 1289, 1301, 1366, 1862, 1915, 1979
AsuII	TTCGAA	1 cut(s)	473
AsuNHI	GCTAGC	1 cut(s)	1942
AvaII	GGWCC	5 cut(s)	199, 1244, 1289, 1366, 1915
BaeI	ACNNNNGTAYC	2 cut(s)	2263, 2296
BamHI	GGATCC	3 cut(s)	328, 943, 1690
BanI	GGYRCC	1 cut(s)	1705
BanII	GRGCYC	1 cut(s)	831
BauI	CACGAG	1 cut(s)	1910
BbsI	GAAGAC	4 cut(s)	668, 1012, 1670, 1793
Bbv12I	GWGCWC	2 cut(s)	831, 2590
BbvI	GCAGC	2 cut(s)	1933, 2429
BccI	CCATC	6 cut(s)	761, 1327, 1550, 1847, 2317, 2463
BceAI	ACGGC	3 cut(s)	1838, 2505, 2539
BcgI	CGANNNNNNTGC	4 cut(s)	1622, 1656, 2516, 2550
BciT130I	CCWGG	2 cut(s)	1891, 2505
BcoDI	GTCTC	7 cut(s)	265, 412, 724, 794, 1852, 2364, 2580
BcuI	ACTAGT	2 cut(s)	421, 1072
BfaI	CTAG	8 cut(s)	152, 422, 459, 836, 1073, 1119, 1296, 1943
BfmI	CTRYAG	2 cut(s)	1947, 2100
BfrI	CTTAAG	1 cut(s)	1526
BfuAI	ACCTGC	2 cut(s)	1030, 2093
BisI	GCNGC	2 cut(s)	1947, 2418
BlsI	GCNGC	2 cut(s)	1948, 2419
BmcAI	AGTACT	2 cut(s)	426, 1532
Bme1390I	CCNGG	2 cut(s)	1891, 2505
Bme18I	GGWCC	5 cut(s)	199, 1244, 1289, 1366, 1915
BmgT120I	GGNCC	8 cut(s)	199, 1244, 1289, 1301, 1366, 1862, 1915, 1979
BmrFI	CCNGG	2 cut(s)	1891, 2505
BmrI	ACTGGG	4 cut(s)	333, 540, 1543, 2162
BmsI	GCATC	2 cut(s)	1586, 1877
BmtI	GCTAGC	1 cut(s)	1946
BmuI	ACTGGG	4 cut(s)	333, 540, 1543, 2162
BpiI	GAAGAC	4 cut(s)	668, 1012, 1670, 1793
Bpu10I	CCTNAGC	1 cut(s)	72
Bpu14I	TTCGAA	1 cut(s)	473
Bsa29I	ATCGAT	1 cut(s)	853
BsaAI	YACGTR	1 cut(s)	1444
BsaBI	GATNNNNATC	1 cut(s)	837
BsaI	GGTCTC	1 cut(s)	794
BsaJI	CCNNGG	1 cut(s)	1696
BsaWI	WCCGGW	3 cut(s)	266, 331, 1898
Bsc4I	CCNNNNNNNGG	7 cut(s)	530, 1298, 1702, 2388, 2389, 2390, 2504
Bse118I	RCCGGY	2 cut(s)	266, 2545
Bse3DI	GCAATG	1 cut(s)	960
Bse8I	GATNNNNATC	1 cut(s)	837
BseAI	TCCGGA	1 cut(s)	1898
BseBI	CCWGG	2 cut(s)	1891, 2505
BseCI	ATCGAT	1 cut(s)	853
BseDI	CCNNGG	1 cut(s)	1696
BseGI	GGATG	4 cut(s)	224, 1868, 2309, 2474
BseJI	GATNNNNATC	1 cut(s)	837
BseLI	CCNNNNNNNGG	7 cut(s)	530, 1298, 1702, 2388, 2389, 2390, 2504
BseMI	GCAATG	1 cut(s)	960
BseMII	CTCAG	8 cut(s)	419, 789, 1389, 1533, 1778, 1819, 1863, 1946
BseXI	GCAGC	2 cut(s)	1933, 2429
BseYI	CCCAGC	1 cut(s)	493
BsgI	GTGCAG	1 cut(s)	2436
Bsh1285I	CGRYCG	1 cut(s)	2227
BshFI	GGCC	6 cut(s)	35, 190, 1051, 1303, 1864, 1981
BshNI	GGYRCC	1 cut(s)	1705
BshTI	ACCGGT	1 cut(s)	266
BshVI	ATCGAT	1 cut(s)	853
BsiEI	CGRYCG	1 cut(s)	2227
BsiHKAI	GWGCWC	2 cut(s)	831, 2590
BsiSI	CCGG	5 cut(s)	267, 332, 1899, 2456, 2546
BsiWI	CGTACG	2 cut(s)	2358, 2562
BslFI	GGGAC	4 cut(s)	212, 547, 997, 1853
BslI	CCNNNNNNNGG	7 cut(s)	530, 1298, 1702, 2388, 2389, 2390, 2504
BsmAI	GTCTC	7 cut(s)	265, 412, 724, 794, 1852, 2364, 2580
BsmBI	CGTCTC	2 cut(s)	1852, 2364
BsmFI	GGGAC	4 cut(s)	212, 547, 997, 1853
BsmI	GAATGC	1 cut(s)	1204
BsnI	GGCC	6 cut(s)	35, 190, 1051, 1303, 1864, 1981
Bso31I	GGTCTC	1 cut(s)	794
Bsp119I	TTCGAA	1 cut(s)	473
Bsp1286I	GDGCHC	2 cut(s)	831, 2590
Bsp13I	TCCGGA	1 cut(s)	1898
BspACI	CCGC	6 cut(s)	14, 618, 1622, 1995, 2218, 2549
BspANI	GGCC	6 cut(s)	35, 190, 1051, 1303, 1864, 1981
BspCNI	CTCAG	8 cut(s)	420, 790, 1390, 1534, 1779, 1818, 1862, 1947
BspDI	ATCGAT	1 cut(s)	853
BspEI	TCCGGA	1 cut(s)	1898
BspMAI	CTGCAG	2 cut(s)	1951, 2104
BspMI	ACCTGC	2 cut(s)	1030, 2093
BspOI	GCTAGC	1 cut(s)	1946
BspQI	GCTCTTC	1 cut(s)	2499
BspT104I	TTCGAA	1 cut(s)	473
BspT107I	GGYRCC	1 cut(s)	1705
BspTI	CTTAAG	1 cut(s)	1526
BspTNI	GGTCTC	1 cut(s)	794
BsrDI	GCAATG	1 cut(s)	960
BsrFI	RCCGGY	2 cut(s)	266, 2545
BssAI	RCCGGY	2 cut(s)	266, 2545
BssECI	CCNNGG	1 cut(s)	1696
BssSI	CACGAG	1 cut(s)	1910
Bst2BI	CACGAG	1 cut(s)	1910
Bst2UI	CCWGG	2 cut(s)	1891, 2505
Bst4CI	ACNGT	6 cut(s)	113, 203, 737, 1685, 2437, 2582
Bst6I	CTCTTC	4 cut(s)	15, 1730, 2231, 2499
BstAFI	CTTAAG	1 cut(s)	1526
BstBAI	YACGTR	1 cut(s)	1444
BstBI	TTCGAA	1 cut(s)	473
BstC8I	GCNNGC	5 cut(s)	873, 1144, 1866, 1944, 2528
BstDSI	CCRYGG	1 cut(s)	1696
BstENI	CCTNNNNNAGG	1 cut(s)	1296
BstF5I	GGATG	4 cut(s)	224, 1868, 2309, 2474
BstMAI	GTCTC	7 cut(s)	265, 412, 724, 794, 1852, 2364, 2580
BstMCI	CGRYCG	1 cut(s)	2227
BstMWI	GCNNNNNNNGC	6 cut(s)	32, 250, 368, 1475, 2414, 2452
BstNI	CCWGG	2 cut(s)	1891, 2505
BstNSI	RCATGY	1 cut(s)	875
BstSCI	CCNGG	2 cut(s)	1889, 2503
BstSFI	CTRYAG	2 cut(s)	1947, 2100
BstV1I	GCAGC	2 cut(s)	1933, 2429
BstV2I	GAAGAC	4 cut(s)	668, 1012, 1670, 1793
BstX2I	RGATCY	5 cut(s)	328, 838, 943, 1690, 2305
BstYI	RGATCY	5 cut(s)	328, 838, 943, 1690, 2305
Bsu15I	ATCGAT	1 cut(s)	853
BsuRI	GGCC	6 cut(s)	35, 190, 1051, 1303, 1864, 1981
BsuTUI	ATCGAT	1 cut(s)	853
BtgI	CCRYGG	1 cut(s)	1696
BtgZI	GCGATG	2 cut(s)	1660, 2173
BtsCI	GGATG	4 cut(s)	224, 1868, 2309, 2474
BtsI	GCAGTG	1 cut(s)	2450
BveI	ACCTGC	2 cut(s)	1030, 2093
Cac8I	GCNNGC	5 cut(s)	873, 1144, 1866, 1944, 2528
CaiI	CAGNNNCTG	4 cut(s)	323, 626, 2435, 2537
Cfr10I	RCCGGY	2 cut(s)	266, 2545
Cfr13I	GGNCC	8 cut(s)	199, 1244, 1289, 1301, 1366, 1862, 1915, 1979
ClaI	ATCGAT	1 cut(s)	853
CseI	GACGC	2 cut(s)	632, 864
CspAI	ACCGGT	1 cut(s)	266
CviAII	CATG	8 cut(s)	119, 872, 891, 980, 1349, 2077, 2329, 2388
DraI	TTTAAA	1 cut(s)	1183
Eam1104I	CTCTTC	4 cut(s)	15, 1730, 2231, 2499
EarI	CTCTTC	4 cut(s)	15, 1730, 2231, 2499
EciI	GGCGGA	2 cut(s)	1611, 2233
Ecl136II	GAGCTC	1 cut(s)	829
Eco147I	AGGCCT	2 cut(s)	35, 190
Eco24I	GRGCYC	1 cut(s)	831
Eco31I	GGTCTC	1 cut(s)	794
Eco32I	GATATC	1 cut(s)	1426
Eco47I	GGWCC	5 cut(s)	199, 1244, 1289, 1366, 1915
Eco53kI	GAGCTC	1 cut(s)	829
Eco57I	CTGAAG	3 cut(s)	300, 2216, 2395
EcoICRI	GAGCTC	1 cut(s)	829
EcoNI	CCTNNNNNAGG	1 cut(s)	1296
EcoO109I	RGGNCCY	3 cut(s)	1301, 1366, 1979
EcoRII	CCWGG	2 cut(s)	1889, 2503
EcoRV	GATATC	1 cut(s)	1426
EcoT22I	ATGCAT	2 cut(s)	892, 1579
EcoT38I	GRGCYC	1 cut(s)	831
Esp3I	CGTCTC	2 cut(s)	1852, 2364
FaeI	CATG	8 cut(s)	122, 875, 894, 983, 1352, 2080, 2332, 2391
FalI	AAGNNNNNCTT	2 cut(s)	2228, 2260
FaqI	GGGAC	4 cut(s)	212, 547, 997, 1853
FatI	CATG	8 cut(s)	118, 871, 890, 979, 1348, 2076, 2328, 2387
FauI	CCCGC	2 cut(s)	625, 2542
FauNDI	CATATG	2 cut(s)	844, 949
FblI	GTMKAC	1 cut(s)	2484
Fnu4HI	GCNGC	2 cut(s)	1947, 2418
FokI	GGATG	4 cut(s)	211, 1855, 2296, 2481
FriOI	GRGCYC	1 cut(s)	831
Fsp4HI	GCNGC	2 cut(s)	1947, 2418
FspBI	CTAG	8 cut(s)	152, 422, 459, 836, 1073, 1119, 1296, 1943
GluI	GCNGC	2 cut(s)	1947, 2418
GsaI	CCCAGC	1 cut(s)	497
HaeIII	GGCC	6 cut(s)	35, 190, 1051, 1303, 1864, 1981
HapII	CCGG	5 cut(s)	267, 332, 1899, 2456, 2546
HgaI	GACGC	2 cut(s)	632, 864
Hin1II	CATG	8 cut(s)	122, 875, 894, 983, 1352, 2080, 2332, 2391
HincII	GTYRAC	1 cut(s)	2485
HindII	GTYRAC	1 cut(s)	2485
HindIII	AAGCTT	2 cut(s)	2133, 2605
HinfI	GANTC	4 cut(s)	258, 452, 2363, 2375
HpaII	CCGG	5 cut(s)	267, 332, 1899, 2456, 2546
Hpy166II	GTNNAC	4 cut(s)	552, 1447, 1915, 2485
Hpy188I	TCNGA	9 cut(s)	482, 609, 1289, 1399, 1788, 1852, 2028, 2235, 2431
Hpy188III	TCNNGA	9 cut(s)	228, 405, 557, 762, 836, 1660, 1899, 2248, 2367
Hpy8I	GTNNAC	4 cut(s)	552, 1447, 1915, 2485
Hpy99I	CGWCG	1 cut(s)	1358
HpyAV	CCTTC	8 cut(s)	175, 480, 598, 920, 1970, 2394, 2419, 2453
HpyCH4III	ACNGT	6 cut(s)	113, 203, 737, 1685, 2437, 2582
HpyCH4IV	ACGT	3 cut(s)	1099, 1443, 1551
HpyF10VI	GCNNNNNNNGC	6 cut(s)	32, 250, 368, 1475, 2414, 2452
HpySE526I	ACGT	3 cut(s)	1099, 1443, 1551
Hsp92II	CATG	8 cut(s)	122, 875, 894, 983, 1352, 2080, 2332, 2391
Kpn2I	TCCGGA	1 cut(s)	1898
KpnI	GGTACC	1 cut(s)	1709
LguI	GCTCTTC	1 cut(s)	2499
LmnI	GCTCC	5 cut(s)	250, 712, 826, 1174, 2220
Lsp1109I	GCAGC	2 cut(s)	1933, 2429
LweI	GCATC	2 cut(s)	1586, 1877
MaeI	CTAG	8 cut(s)	152, 422, 459, 836, 1073, 1119, 1296, 1943
MaeII	ACGT	3 cut(s)	1099, 1443, 1551
MaeIII	GTNAC	6 cut(s)	635, 860, 1487, 1537, 1808, 2393
MfeI	CAATTG	2 cut(s)	1638, 1963
MflI	RGATCY	5 cut(s)	328, 838, 943, 1690, 2305
MhlI	GDGCHC	2 cut(s)	831, 2590
MlyI	GAGTC	1 cut(s)	267
MmeI	TCCRAC	2 cut(s)	804, 1152
Mph1103I	ATGCAT	2 cut(s)	892, 1579
MroI	TCCGGA	1 cut(s)	1898
MroXI	GAANNNNTTC	2 cut(s)	1100, 1283
MseI	TTAA	6 cut(s)	1058, 1182, 1527, 2016, 2073, 2198
MslI	CAYNNNNRTG	4 cut(s)	123, 1322, 1554, 2583
MspA1I	CMGCKG	5 cut(s)	16, 497, 809, 1997, 2420
MspCI	CTTAAG	1 cut(s)	1526
MspI	CCGG	5 cut(s)	267, 332, 1899, 2456, 2546
MspR9I	CCNGG	2 cut(s)	1891, 2505
MunI	CAATTG	2 cut(s)	1638, 1963
Mva1269I	GAATGC	1 cut(s)	1204
MvaI	CCWGG	2 cut(s)	1891, 2505
MwoI	GCNNNNNNNGC	6 cut(s)	32, 250, 368, 1475, 2414, 2452
NdeI	CATATG	2 cut(s)	844, 949
NheI	GCTAGC	1 cut(s)	1942
NlaIII	CATG	8 cut(s)	122, 875, 894, 983, 1352, 2080, 2332, 2391
NmeAIII	GCCGAG	1 cut(s)	2037
NmuCI	GTSAC	5 cut(s)	635, 860, 1537, 1808, 2393
NsiI	ATGCAT	2 cut(s)	892, 1579
NspI	RCATGY	1 cut(s)	875
NspV	TTCGAA	1 cut(s)	473
OliI	CACNNNNGTG	1 cut(s)	2583
PaeI	GCATGC	1 cut(s)	875
PaqCI	CACCTGC	1 cut(s)	2093
PceI	AGGCCT	2 cut(s)	35, 190
PciSI	GCTCTTC	1 cut(s)	2499
PcsI	WCGNNNNNNNCGW	2 cut(s)	1842, 1917
PctI	GAATGC	1 cut(s)	1204
PdmI	GAANNNNTTC	2 cut(s)	1100, 1283
PfeI	GAWTC	3 cut(s)	452, 2363, 2375
Pfl23II	CGTACG	2 cut(s)	2358, 2562
PflFI	GACNNNGTC	2 cut(s)	988, 1844
PflMI	CCANNNNNTGG	1 cut(s)	530
PfoI	TCCNGGA	1 cut(s)	1889
PinAI	ACCGGT	1 cut(s)	266
PkrI	GCNGC	2 cut(s)	1948, 2419
PleI	GAGTC	1 cut(s)	266
PpsI	GAGTC	1 cut(s)	266
Ppu21I	YACGTR	1 cut(s)	1444
PpuMI	RGGWCCY	1 cut(s)	1366
PsiI	TTATAA	1 cut(s)	1571
Psp124BI	GAGCTC	1 cut(s)	831
Psp1406I	AACGTT	1 cut(s)	1099
Psp5II	RGGWCCY	1 cut(s)	1366
Psp6I	CCWGG	2 cut(s)	1889, 2503
PspFI	CCCAGC	1 cut(s)	493
PspGI	CCWGG	2 cut(s)	1889, 2503
PspLI	CGTACG	2 cut(s)	2358, 2562
PspPI	GGNCC	8 cut(s)	199, 1244, 1289, 1301, 1366, 1862, 1915, 1979
PspPPI	RGGWCCY	1 cut(s)	1366
PstI	CTGCAG	2 cut(s)	1951, 2104
PstNI	CAGNNNCTG	4 cut(s)	323, 626, 2435, 2537
PsuI	RGATCY	5 cut(s)	328, 838, 943, 1690, 2305
PsyI	GACNNNGTC	2 cut(s)	988, 1844
PvuII	CAGCTG	3 cut(s)	497, 809, 2420
RseI	CAYNNNNRTG	4 cut(s)	123, 1322, 1554, 2583
SacI	GAGCTC	1 cut(s)	831
SalI	GTCGAC	1 cut(s)	2483
SapI	GCTCTTC	1 cut(s)	2499
SaqAI	TTAA	6 cut(s)	1058, 1182, 1527, 2016, 2073, 2198
SatI	GCNGC	2 cut(s)	1947, 2418
Sau96I	GGNCC	8 cut(s)	199, 1244, 1289, 1301, 1366, 1862, 1915, 1979
ScaI	AGTACT	2 cut(s)	426, 1532
SchI	GAGTC	1 cut(s)	267
ScrFI	CCNGG	2 cut(s)	1891, 2505
SduI	GDGCHC	2 cut(s)	831, 2590
SfaNI	GCATC	2 cut(s)	1586, 1877
SfcI	CTRYAG	2 cut(s)	1947, 2100
SfuI	TTCGAA	1 cut(s)	473
SinI	GGWCC	5 cut(s)	199, 1244, 1289, 1366, 1915
SmiMI	CAYNNNNRTG	4 cut(s)	123, 1322, 1554, 2583
SmlI	CTYRAG	1 cut(s)	1526
SmoI	CTYRAG	1 cut(s)	1526
SpeI	ACTAGT	2 cut(s)	421, 1072
SphI	GCATGC	1 cut(s)	875
SseBI	AGGCCT	2 cut(s)	35, 190
SsiI	CCGC	6 cut(s)	14, 618, 1622, 1995, 2218, 2549
SspMI	CTAG	8 cut(s)	152, 422, 459, 836, 1073, 1119, 1296, 1943
SstI	GAGCTC	1 cut(s)	831
StuI	AGGCCT	2 cut(s)	35, 190
StyD4I	CCNGG	2 cut(s)	1889, 2503
TaaI	ACNGT	6 cut(s)	113, 203, 737, 1685, 2437, 2582
TaiI	ACGT	3 cut(s)	1102, 1446, 1554
TaqII	GACCGA	1 cut(s)	2241
TatI	WGTACW	3 cut(s)	424, 1530, 1999
TfiI	GAWTC	3 cut(s)	452, 2363, 2375
Tru1I	TTAA	6 cut(s)	1058, 1182, 1527, 2016, 2073, 2198
Tru9I	TTAA	6 cut(s)	1058, 1182, 1527, 2016, 2073, 2198
TseFI	GTSAC	5 cut(s)	635, 860, 1537, 1808, 2393
TseI	GCWGC	2 cut(s)	1946, 2417
Tsp45I	GTSAC	5 cut(s)	635, 860, 1537, 1808, 2393
TspGWI	ACGGA	3 cut(s)	101, 1371, 2106
Tth111I	GACNNNGTC	2 cut(s)	988, 1844
Van91I	CCANNNNNTGG	1 cut(s)	530
Vha464I	CTTAAG	1 cut(s)	1526
VpaK11BI	GGWCC	5 cut(s)	199, 1244, 1289, 1366, 1915
XagI	CCTNNNNNAGG	1 cut(s)	1296
XapI	RAATTY	7 cut(s)	174, 475, 667, 1341, 1760, 2022, 2318
XbaI	TCTAGA	1 cut(s)	835
XceI	RCATGY	1 cut(s)	875
XmiI	GTMKAC	1 cut(s)	2484
XmnI	GAANNNNTTC	2 cut(s)	1100, 1283
XspI	CTAG	8 cut(s)	152, 422, 459, 836, 1073, 1119, 1296, 1943
ZrmI	AGTACT	2 cut(s)	426, 1532
Zsp2I	ATGCAT	2 cut(s)	892, 1579

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.