Isoamylase 2
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G03310 AT1G03310 |
| fragaria_vesca | FvH4_2g23950 |
| malus_domestica | MD03G1268800.v1.1 MD11G1289600.v1.1 |
| prunus_persica | Prupe.8G244800_v2.0.a1 |
| pyrus_communis | pycom11g25700 |
| rosa_chinensis | RchiOBHm_Chr6g0291581 |
| rosa_laevigata | RLG00000012105 |
| rosa_multiflora | Rmu_sc0002226.1_g000007 |
| rosa_roxburghii | Rroxscaffold_7G00175950 |
| rosa_rugosa | Rorug06G0220400 Rorug06G0220400 |
| rosa_samantha | Rh6AG332800 Rh6BG339600 Rh6CG346000 Rh6DG333200 |
| rosa_wichuraiana | Rw6G028900 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1062 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 413 |
| Acc36I | ACCTGC | 2 cut(s) | 1062, 1130 |
| Acc65I | GGTACC | 1 cut(s) | 1702 |
| AccB1I | GGYRCC | 1 cut(s) | 1702 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2504 |
| AciI | CCGC | 5 cut(s) | 169, 191, 1397, 1619, 2215 |
| AclWI | GGATC | 7 cut(s) | 169, 327, 791, 929, 942, 1179, 1640 |
| AcsI | RAATTY | 5 cut(s) | 586, 625, 1471, 1891, 2315 |
| AcuI | CTGAAG | 7 cut(s) | 291, 447, 483, 1929, 2082, 2213, 2395 |
| AcyI | GRCGYC | 2 cut(s) | 2008, 2058 |
| AfaI | GTAC | 6 cut(s) | 268, 752, 1144, 1447, 1704, 1998 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 42, 424, 1295, 2459, 2504 |
| AflIII | ACRYGT | 1 cut(s) | 1094 |
| AjiI | CACGTC | 1 cut(s) | 1549 |
| AjnI | CCWGG | 1 cut(s) | 2503 |
| AleI | CACNNNNGTG | 1 cut(s) | 2583 |
| Alw21I | GWGCWC | 2 cut(s) | 1110, 2590 |
| Alw26I | GTCTC | 5 cut(s) | 90, 458, 640, 2426, 2580 |
| AlwI | GGATC | 7 cut(s) | 169, 327, 791, 929, 942, 1179, 1640 |
| AoxI | GGCC | 5 cut(s) | 33, 45, 1297, 1858, 1976 |
| ApeKI | GCWGC | 5 cut(s) | 10, 1394, 1408, 1501, 2417 |
| ApoI | RAATTY | 5 cut(s) | 586, 625, 1471, 1891, 2315 |
| Asp718I | GGTACC | 1 cut(s) | 1702 |
| AvaII | GGWCC | 6 cut(s) | 885, 1241, 1286, 1363, 1687, 1912 |
| BamHI | GGATCC | 1 cut(s) | 934 |
| BanI | GGYRCC | 1 cut(s) | 1702 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2225, 2257 |
| BbsI | GAAGAC | 6 cut(s) | 626, 1009, 1514, 1790, 1892, 2307 |
| Bbv12I | GWGCWC | 2 cut(s) | 1110, 2590 |
| BbvI | GCAGC | 4 cut(s) | 1381, 1395, 1513, 2429 |
| BceAI | ACGGC | 3 cut(s) | 1835, 2460, 2505 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2126, 2160 |
| BciT130I | CCWGG | 1 cut(s) | 2505 |
| BcoDI | GTCTC | 5 cut(s) | 90, 458, 640, 2426, 2580 |
| BfaI | CTAG | 5 cut(s) | 149, 413, 687, 794, 2000 |
| BfmI | CTRYAG | 4 cut(s) | 29, 358, 871, 1302 |
| BfuAI | ACCTGC | 2 cut(s) | 1062, 1130 |
| BglII | AGATCT | 1 cut(s) | 1183 |
| BisI | GCNGC | 6 cut(s) | 11, 191, 1395, 1409, 1502, 2418 |
| BlsI | GCNGC | 6 cut(s) | 12, 192, 1396, 1410, 1503, 2419 |
| Bme1390I | CCNGG | 1 cut(s) | 2505 |
| Bme18I | GGWCC | 6 cut(s) | 885, 1241, 1286, 1363, 1687, 1912 |
| BmgBI | CACGTC | 1 cut(s) | 1549 |
| BmrFI | CCNGG | 1 cut(s) | 2505 |
| BmrI | ACTGGG | 2 cut(s) | 1039, 1540 |
| BmsI | GCATC | 2 cut(s) | 571, 1583 |
| BmuI | ACTGGG | 2 cut(s) | 1039, 1540 |
| BpiI | GAAGAC | 6 cut(s) | 626, 1009, 1514, 1790, 1892, 2307 |
| BpmI | CTGGAG | 3 cut(s) | 190, 803, 1314 |
| Bpu10I | CCTNAGC | 2 cut(s) | 945, 1452 |
| Bsa29I | ATCGAT | 1 cut(s) | 811 |
| BsaAI | YACGTR | 1 cut(s) | 1589 |
| BsaBI | GATNNNNATC | 1 cut(s) | 795 |
| BsaHI | GRCGYC | 2 cut(s) | 2008, 2058 |
| BsaJI | CCNNGG | 1 cut(s) | 2291 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 150, 180, 239, 269, 1287, 1317, 2203, 2233 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 42, 424, 1295, 2459, 2504 |
| Bse1I | ACTGG | 8 cut(s) | 268, 450, 1034, 1116, 1374, 1535, 2144, 2270 |
| Bse3DI | GCAATG | 4 cut(s) | 12, 1671, 1771, 2154 |
| Bse8I | GATNNNNATC | 1 cut(s) | 795 |
| BseBI | CCWGG | 1 cut(s) | 2505 |
| BseCI | ATCGAT | 1 cut(s) | 811 |
| BseDI | CCNNGG | 1 cut(s) | 2291 |
| BseGI | GGATG | 3 cut(s) | 446, 1039, 1489 |
| BseJI | GATNNNNATC | 1 cut(s) | 795 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 42, 424, 1295, 2459, 2504 |
| BseMI | GCAATG | 4 cut(s) | 12, 1671, 1771, 2154 |
| BseMII | CTCAG | 7 cut(s) | 433, 651, 782, 884, 1095, 1530, 1943 |
| BseNI | ACTGG | 8 cut(s) | 268, 450, 1034, 1116, 1374, 1535, 2144, 2270 |
| BseRI | GAGGAG | 1 cut(s) | 2224 |
| BseXI | GCAGC | 4 cut(s) | 1381, 1395, 1513, 2429 |
| BseYI | CCCAGC | 2 cut(s) | 1683, 1861 |
| BsgI | GTGCAG | 2 cut(s) | 1520, 2436 |
| BshFI | GGCC | 5 cut(s) | 35, 47, 1299, 1860, 1978 |
| BshNI | GGYRCC | 1 cut(s) | 1702 |
| BshVI | ATCGAT | 1 cut(s) | 811 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1110, 2590 |
| BslFI | GGGAC | 4 cut(s) | 898, 1700, 1850, 2045 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 42, 424, 1295, 2459, 2504 |
| BsmAI | GTCTC | 5 cut(s) | 90, 458, 640, 2426, 2580 |
| BsmFI | GGGAC | 4 cut(s) | 898, 1700, 1850, 2045 |
| BsmI | GAATGC | 3 cut(s) | 1756, 1865, 2149 |
| BsnI | GGCC | 5 cut(s) | 35, 47, 1299, 1860, 1978 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1110, 2590 |
| BspACI | CCGC | 5 cut(s) | 169, 191, 1397, 1619, 2215 |
| BspANI | GGCC | 5 cut(s) | 35, 47, 1299, 1860, 1978 |
| BspCNI | CTCAG | 7 cut(s) | 432, 650, 781, 885, 1096, 1531, 1944 |
| BspDI | ATCGAT | 1 cut(s) | 811 |
| BspHI | TCATGA | 1 cut(s) | 1693 |
| BspMI | ACCTGC | 2 cut(s) | 1062, 1130 |
| BspPI | GGATC | 7 cut(s) | 169, 327, 791, 929, 942, 1179, 1640 |
| BspT107I | GGYRCC | 1 cut(s) | 1702 |
| BsrDI | GCAATG | 4 cut(s) | 12, 1671, 1771, 2154 |
| BsrI | ACTGG | 8 cut(s) | 268, 450, 1034, 1116, 1374, 1535, 2144, 2270 |
| BssECI | CCNNGG | 1 cut(s) | 2291 |
| BssNI | GRCGYC | 2 cut(s) | 2008, 2058 |
| Bst2UI | CCWGG | 1 cut(s) | 2505 |
| Bst4CI | ACNGT | 8 cut(s) | 352, 362, 565, 1601, 2120, 2427, 2437, 2582 |
| Bst6I | CTCTTC | 1 cut(s) | 1727 |
| BstACI | GRCGYC | 2 cut(s) | 2008, 2058 |
| BstBAI | YACGTR | 1 cut(s) | 1589 |
| BstC8I | GCNNGC | 4 cut(s) | 216, 777, 831, 1619 |
| BstF5I | GGATG | 3 cut(s) | 446, 1039, 1489 |
| BstMAI | GTCTC | 5 cut(s) | 90, 458, 640, 2426, 2580 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 1448, 2414 |
| BstNI | CCWGG | 1 cut(s) | 2505 |
| BstNSI | RCATGY | 2 cut(s) | 433, 833 |
| BstSCI | CCNGG | 1 cut(s) | 2503 |
| BstSFI | CTRYAG | 4 cut(s) | 29, 358, 871, 1302 |
| BstV1I | GCAGC | 4 cut(s) | 1381, 1395, 1513, 2429 |
| BstV2I | GAAGAC | 6 cut(s) | 626, 1009, 1514, 1790, 1892, 2307 |
| BstX2I | RGATCY | 5 cut(s) | 161, 319, 796, 934, 1183 |
| BstYI | RGATCY | 5 cut(s) | 161, 319, 796, 934, 1183 |
| Bsu15I | ATCGAT | 1 cut(s) | 811 |
| BsuRI | GGCC | 5 cut(s) | 35, 47, 1299, 1860, 1978 |
| BsuTUI | ATCGAT | 1 cut(s) | 811 |
| BtrI | CACGTC | 1 cut(s) | 1549 |
| BtsCI | GGATG | 3 cut(s) | 446, 1039, 1489 |
| BtsI | GCAGTG | 3 cut(s) | 406, 762, 1939 |
| BtsIMutI | CAGTG | 9 cut(s) | 57, 261, 367, 406, 762, 1933, 1939, 2125, 2419 |
| BveI | ACCTGC | 2 cut(s) | 1062, 1130 |
| Cac8I | GCNNGC | 4 cut(s) | 216, 777, 831, 1619 |
| CciI | TCATGA | 1 cut(s) | 1693 |
| ClaI | ATCGAT | 1 cut(s) | 811 |
| CseI | GACGC | 4 cut(s) | 143, 822, 2016, 2047 |
| Csp6I | GTAC | 6 cut(s) | 267, 751, 1143, 1446, 1703, 1997 |
| CviQI | GTAC | 6 cut(s) | 267, 751, 1143, 1446, 1703, 1997 |
| DraI | TTTAAA | 1 cut(s) | 1180 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 413 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 413 |
| Eam1104I | CTCTTC | 1 cut(s) | 1727 |
| EarI | CTCTTC | 1 cut(s) | 1727 |
| EciI | GGCGGA | 1 cut(s) | 2230 |
| Eco147I | AGGCCT | 1 cut(s) | 35 |
| Eco47I | GGWCC | 6 cut(s) | 885, 1241, 1286, 1363, 1687, 1912 |
| Eco57I | CTGAAG | 7 cut(s) | 291, 447, 483, 1929, 2082, 2213, 2395 |
| EcoO109I | RGGNCCY | 5 cut(s) | 885, 1298, 1363, 1687, 1976 |
| EcoRII | CCWGG | 1 cut(s) | 2503 |
| EcoT22I | ATGCAT | 1 cut(s) | 1576 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1459, 1491, 2228, 2260 |
| FaqI | GGGAC | 4 cut(s) | 898, 1700, 1850, 2045 |
| FauI | CCCGC | 2 cut(s) | 1390, 1626 |
| FauNDI | CATATG | 2 cut(s) | 824, 940 |
| Fnu4HI | GCNGC | 6 cut(s) | 11, 191, 1395, 1409, 1502, 2418 |
| FokI | GGATG | 3 cut(s) | 433, 1046, 1476 |
| Fsp4HI | GCNGC | 6 cut(s) | 11, 191, 1395, 1409, 1502, 2418 |
| FspBI | CTAG | 5 cut(s) | 149, 413, 687, 794, 2000 |
| GluI | GCNGC | 6 cut(s) | 11, 191, 1395, 1409, 1502, 2418 |
| GsaI | CCCAGC | 2 cut(s) | 1687, 1865 |
| GsuI | CTGGAG | 3 cut(s) | 190, 803, 1314 |
| HaeIII | GGCC | 5 cut(s) | 35, 47, 1299, 1860, 1978 |
| HgaI | GACGC | 4 cut(s) | 143, 822, 2016, 2047 |
| Hin1I | GRCGYC | 2 cut(s) | 2008, 2058 |
| HincII | GTYRAC | 3 cut(s) | 355, 1597, 2485 |
| HindII | GTYRAC | 3 cut(s) | 355, 1597, 2485 |
| HinfI | GANTC | 5 cut(s) | 62, 443, 959, 1416, 1758 |
| HpaI | GTTAAC | 1 cut(s) | 1597 |
| Hpy166II | GTNNAC | 6 cut(s) | 355, 1195, 1597, 1912, 2090, 2485 |
| Hpy188I | TCNGA | 7 cut(s) | 466, 771, 894, 1849, 1948, 2232, 2431 |
| Hpy8I | GTNNAC | 6 cut(s) | 355, 1195, 1597, 1912, 2090, 2485 |
| Hpy99I | CGWCG | 1 cut(s) | 1553 |
| HpyAV | CCTTC | 6 cut(s) | 471, 899, 911, 2329, 2419, 2453 |
| HpyCH4III | ACNGT | 8 cut(s) | 352, 362, 565, 1601, 2120, 2427, 2437, 2582 |
| HpyCH4IV | ACGT | 4 cut(s) | 1096, 1145, 1548, 1588 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 1448, 2414 |
| HpySE526I | ACGT | 4 cut(s) | 1096, 1145, 1548, 1588 |
| Hsp92I | GRCGYC | 2 cut(s) | 2008, 2058 |
| KflI | GGGWCCC | 2 cut(s) | 885, 1687 |
| KpnI | GGTACC | 1 cut(s) | 1706 |
| KspAI | GTTAAC | 1 cut(s) | 1597 |
| LmnI | GCTCC | 3 cut(s) | 247, 784, 2472 |
| Lsp1109I | GCAGC | 4 cut(s) | 1381, 1395, 1513, 2429 |
| LweI | GCATC | 2 cut(s) | 571, 1583 |
| MaeI | CTAG | 5 cut(s) | 149, 413, 687, 794, 2000 |
| MaeII | ACGT | 4 cut(s) | 1096, 1145, 1548, 1588 |
| MfeI | CAATTG | 4 cut(s) | 389, 1635, 2363, 2517 |
| MflI | RGATCY | 5 cut(s) | 161, 319, 796, 934, 1183 |
| MhlI | GDGCHC | 2 cut(s) | 1110, 2590 |
| MmeI | TCCRAC | 4 cut(s) | 979, 1149, 1937, 2409 |
| Mph1103I | ATGCAT | 1 cut(s) | 1576 |
| MseI | TTAA | 7 cut(s) | 879, 1179, 1596, 2013, 2070, 2195, 2387 |
| MslI | CAYNNNNRTG | 3 cut(s) | 1350, 1551, 2583 |
| MspA1I | CMGCKG | 1 cut(s) | 2420 |
| MspR9I | CCNGG | 1 cut(s) | 2505 |
| MunI | CAATTG | 4 cut(s) | 389, 1635, 2363, 2517 |
| Mva1269I | GAATGC | 3 cut(s) | 1756, 1865, 2149 |
| MvaI | CCWGG | 1 cut(s) | 2505 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 1448, 2414 |
| NdeI | CATATG | 2 cut(s) | 824, 940 |
| NmuCI | GTSAC | 6 cut(s) | 200, 818, 1534, 2120, 2276, 2393 |
| NsiI | ATGCAT | 1 cut(s) | 1576 |
| NspI | RCATGY | 2 cut(s) | 433, 833 |
| OliI | CACNNNNGTG | 1 cut(s) | 2583 |
| PaeI | GCATGC | 1 cut(s) | 833 |
| PagI | TCATGA | 1 cut(s) | 1693 |
| PaqCI | CACCTGC | 1 cut(s) | 1062 |
| PceI | AGGCCT | 1 cut(s) | 35 |
| PctI | GAATGC | 3 cut(s) | 1756, 1865, 2149 |
| PfeI | GAWTC | 5 cut(s) | 62, 443, 959, 1416, 1758 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2504 |
| PfoI | TCCNGGA | 1 cut(s) | 2503 |
| PkrI | GCNGC | 6 cut(s) | 12, 192, 1396, 1410, 1503, 2419 |
| Ppu21I | YACGTR | 1 cut(s) | 1589 |
| PpuMI | RGGWCCY | 3 cut(s) | 885, 1363, 1687 |
| Psp5II | RGGWCCY | 3 cut(s) | 885, 1363, 1687 |
| Psp6I | CCWGG | 1 cut(s) | 2503 |
| PspFI | CCCAGC | 2 cut(s) | 1683, 1861 |
| PspGI | CCWGG | 1 cut(s) | 2503 |
| PspPPI | RGGWCCY | 3 cut(s) | 885, 1363, 1687 |
| PsuI | RGATCY | 5 cut(s) | 161, 319, 796, 934, 1183 |
| PvuII | CAGCTG | 1 cut(s) | 2420 |
| RsaI | GTAC | 6 cut(s) | 268, 752, 1144, 1447, 1704, 1998 |
| RsaNI | GTAC | 6 cut(s) | 267, 751, 1143, 1446, 1703, 1997 |
| RseI | CAYNNNNRTG | 3 cut(s) | 1350, 1551, 2583 |
| SaqAI | TTAA | 7 cut(s) | 879, 1179, 1596, 2013, 2070, 2195, 2387 |
| SatI | GCNGC | 6 cut(s) | 11, 191, 1395, 1409, 1502, 2418 |
| ScrFI | CCNGG | 1 cut(s) | 2505 |
| SduI | GDGCHC | 2 cut(s) | 1110, 2590 |
| SfaNI | GCATC | 2 cut(s) | 571, 1583 |
| SfcI | CTRYAG | 4 cut(s) | 29, 358, 871, 1302 |
| SinI | GGWCC | 6 cut(s) | 885, 1241, 1286, 1363, 1687, 1912 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 1350, 1551, 2583 |
| SphI | GCATGC | 1 cut(s) | 833 |
| SseBI | AGGCCT | 1 cut(s) | 35 |
| SsiI | CCGC | 5 cut(s) | 169, 191, 1397, 1619, 2215 |
| SspI | AATATT | 1 cut(s) | 2260 |
| SspMI | CTAG | 5 cut(s) | 149, 413, 687, 794, 2000 |
| StuI | AGGCCT | 1 cut(s) | 35 |
| StyD4I | CCNGG | 1 cut(s) | 2503 |
| TaaI | ACNGT | 8 cut(s) | 352, 362, 565, 1601, 2120, 2427, 2437, 2582 |
| TaiI | ACGT | 4 cut(s) | 1099, 1148, 1551, 1591 |
| TatI | WGTACW | 2 cut(s) | 1445, 1996 |
| TauI | GCSGC | 1 cut(s) | 193 |
| TfiI | GAWTC | 5 cut(s) | 62, 443, 959, 1416, 1758 |
| Tru1I | TTAA | 7 cut(s) | 879, 1179, 1596, 2013, 2070, 2195, 2387 |
| Tru9I | TTAA | 7 cut(s) | 879, 1179, 1596, 2013, 2070, 2195, 2387 |
| TscAI | CASTG | 9 cut(s) | 64, 268, 367, 406, 769, 1933, 1939, 2125, 2419 |
| TseFI | GTSAC | 6 cut(s) | 200, 818, 1534, 2120, 2276, 2393 |
| TseI | GCWGC | 5 cut(s) | 10, 1394, 1408, 1501, 2417 |
| Tsp45I | GTSAC | 6 cut(s) | 200, 818, 1534, 2120, 2276, 2393 |
| TspGWI | ACGGA | 3 cut(s) | 293, 789, 2012 |
| TspRI | CASTG | 9 cut(s) | 64, 268, 367, 406, 769, 1933, 1939, 2125, 2419 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2504 |
| VpaK11BI | GGWCC | 6 cut(s) | 885, 1241, 1286, 1363, 1687, 1912 |
| XapI | RAATTY | 5 cut(s) | 586, 625, 1471, 1891, 2315 |
| XbaI | TCTAGA | 1 cut(s) | 793 |
| XceI | RCATGY | 2 cut(s) | 433, 833 |
| XspI | CTAG | 5 cut(s) | 149, 413, 687, 794, 2000 |
| Zsp2I | ATGCAT | 1 cut(s) | 1576 |