Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0013348

Physical Location & Seq

Genomic Coordinates Forward (+)

40921333 .. 40924810

Gene Structure 3478 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1289600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2613 bp

ATGGCAACTCTTCCGCTGTCAATCGCCATGCAGGCCTCCTGTTGTTTGAATTGTGGAACCACTGAATTGTCTAAGCTGACTGCTGCAAATCGTTATAGACACAGGCACAATGGTCTGCGTGGTTCTGTAAAACTGGGTATAGAGAGAAATCTAGTTTTCGGAGAAGTTGTGCAGAACTTCAAGGAAACGCCTCTAAGGGACCGTGACTTGAAAGTGTATGCTACATCCCGAGTTTCTGTTGAGCCAATGGAGCAAAGAGTCTACACCAGTACTGAAACTGAAGAAGCAGGCAAGGTATCAACTTATCGATTTAGAACAGAAACTGGGGATATGGTAAAAGTTTTTGTTAGAATGAAGAATGCCAAATGCATTGTGAATATTGAAGTTTCATCGTTGCACCTGTCTAGTAATGATAGATTACTGGTACTGAGTTGGGGAATTTATAGATCTGATTCATCTAGTTTCATGCCTTCGAATTTCAGAAGTTCGACCCCAGCTGACAGAACCACCACCCTTGAAACTCCATTCACTGAGACTTGTTCCGGCAGGTTTACTCTCGAATTGGAGTTTGAGGCTAAACAAATCCCCTTCTACCTCTCGTTTATTTTGAAATCCCCCGCAGATGCTGATTCGAGTGACTTGGACATAAGAAGTCATAGGAAGACAAATTTCTGTTTCCCGGTTGGTTTTGGTCGAGGCTATCCCGCTCCATTGGGTCTCTCTTTTTCTAATGATGGTTCCATGAACTTTGCCATCTTTTCAAGAAATGCAGAAAGTGTAGTTTTATGCTTGTATGGTGAGACCACAGCTGAAAAACCTGTGTTGGAGCTCGATCTAGATCCATATGTTAATCGATCAGGTGACATCTGGCATGCGTCATTTGAAAGTGGATGGGATTTTGTGAGCTATGGTTATAGATTTGATGAAGGGAATGTCTTGCTGGATCCATACGCTAAGATCATTTCGAGATCTGTTCCTCATGGGACAGGGTTGAAGTATCTTGGAAGGCTATGTGAGGAACCTGCTTTCAACTGGGCTGGTGACGTTCGACCTGACTTAGCAATGGAAAAACTTGTGGTTTATCGGTTGAACGTGACACGTTTTACAGAGCATAAGTCTAGTAATCTACCAACCAATATAGCAGGCACTTTTTCGGGTTTGACAGAGAAGTTGGAGCATTTAAAACACCTGGGTGTGAATGCAGTTTTATTAGAGCCTATTTTCCCATTTGACGAGCAAAAGGGTCCATATTTCCCTATCCATTTCTTTTCACCTATGAATTGGTTTGGACCTTCTAGAGGGCCTGTATCAGCTGTCAATTCTATGAAAGACATGGTGAAAAAATTTCATGCCGACGGAATAGAGGTCCTACTGGAAGTTGTTTTCACCCATACCGCTGAGGGTGAAGCTCTGCAAGGAATCGATATCTCATCTTATTATCACATAAACGGGGTTGCGGATTTGAAAGCCAGAAATGCTTTGAATTGTAACTATCCTGTTGTGCAACAAATGGTATTGGATAGTCTTCGATACTGGGTGACTGAGTTTCACGTTGATGGCTTTTGTTTTATAAATGCATCATCTCTGATGCGAGGGTCTAAAGGTGAATACTTATCTCGCCCACCTTTGGTCGAAGCAATTGCTTTTGATCCGCTACTCTCGAAGACCAAGATCATTGCAGACCGCTGGGATCCTCATGGCTCGGTACCAAAAGAAACTCGCTTTCCTCATTGGAAGAGATGGGCAGAAGTGAATTCGAAATTCTCTAAAGATGTGAGGAACTTTTTGAGGGGTGAGGGCCTTCTTAGTGACCTTGCTACACGGCTCTGTGGGAACGGGGACATCTTCTCGGATGGACGAGGGCCAGCATTCGCTTTCAACTTTATTTCCCGGAATTCCGGACTTCCTCTCGTGGACCTTGTTAGTTTCAGTGGTGTTAAGTTAGCTTCAGAGCTGAGTTGGAATTGCGGGGAAGAAGGGCCTACAGATAAAACTGCTGTACTTGAAAGGCGACTTAAACAAATTCGTAATTTTCTCTTTATCTTGTTTGTTTCACTCGGCGTTCCTGTTCTTAACATGGGAGATGAGTGTGGACAATCTACAGGCGGTTCCCCTGCATACAGTGACAGAAAAGCTTTCGATTGGAACGCACTGGGAACAGGTTTTGCTACTCAAACCACACAATTCATTGCGTTTTTAAGTTCGTTTAGGATAAGGCGGAGCGACCTACTTCAGGAGAGGAACTTCTTGAAAGAAGAAAACATAGACTGGTACGGGAGTGATCAATCTTCTCCAAAATGGGAAGATCCGTCCTGCAAATTCCTTGCCATGAAGCTGAAACCAGACGAAGAGGAAGCGACCGAACCAGGAGATGTTTCTCCCCCTATATGGGGTGACTTGTTTGTTGCCTTCAGTGCAGCAGCTCGTTCAGAGACTGTCATTCTGCCTCCGCCTCCGGAAGGTATGGGATGGTTCCGTTTGGTCGACACGGCTCTTCCATTTCCAGGTTTTTTCTCAACCGACGGCGAGCCAGTTCCGGAACAAATGGCGGGTTTGTTTGCGTACCAAATGAAGTCTCACAGTTGTGCTCTGTTTGAAGCCAGATGCTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009251 GO:0009507 GO:0009536 GO:0009987 GO:0010021 GO:0015980 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0032991 GO:0043033 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0071704 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1902494 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

871

Amino Acids

97.39

Weight (kDa)

5.83

Isoelectric Point (pI)

41.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	237 - 318	1.1e-10	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	376 - 467	1.5e-09	Alpha amylase, catalytic domain
ISOA1-3_C	PF21156	756 - 847	1.9e-06	Isoamylase 1-3, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0013348)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03310 AT1G03310
fragaria_vesca	FvH4_2g23950
malus_domestica	MD03G1268800.v1.1 MD11G1289600.v1.1
prunus_persica	Prupe.8G244800_v2.0.a1
pyrus_communis	pycom11g25700
rosa_chinensis	RchiOBHm_Chr6g0291581
rosa_laevigata	RLG00000012105
rosa_multiflora	Rmu_sc0002226.1_g000007
rosa_roxburghii	Rroxscaffold_7G00175950
rosa_rugosa	Rorug06G0220400 Rorug06G0220400
rosa_samantha	Rh6AG332800 Rh6BG339600 Rh6CG346000 Rh6DG333200
rosa_wichuraiana	Rw6G028900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1571
Acc36I	ACCTGC	2 cut(s)	537, 1030
Acc65I	GGTACC	1 cut(s)	1705
AccB1I	GGYRCC	1 cut(s)	1705
AccBSI	CCGCTC	1 cut(s)	707
AccI	GTMKAC	2 cut(s)	261, 2484
AccIII	TCCGGA	3 cut(s)	1898, 2455, 2536
AclWI	GGATC	7 cut(s)	833, 938, 951, 1643, 1685, 1698, 2300
AcsI	RAATTY	9 cut(s)	438, 475, 667, 1343, 1753, 1760, 1894, 2022, 2318
AcuI	CTGAAG	4 cut(s)	300, 1932, 2216, 2395
AfaI	GTAC	6 cut(s)	271, 426, 1707, 2001, 2273, 2564
AfiI	CCNNNNNNNGG	8 cut(s)	1298, 1627, 2233, 2388, 2389, 2390, 2459, 2504
AflIII	ACRYGT	1 cut(s)	1097
AjnI	CCWGG	3 cut(s)	1188, 2365, 2503
AleI	CACNNNNGTG	2 cut(s)	1191, 2583
Alw21I	GWGCWC	2 cut(s)	831, 2590
Alw26I	GTCTC	5 cut(s)	527, 722, 794, 2426, 2580
AlwI	GGATC	7 cut(s)	833, 938, 951, 1643, 1685, 1698, 2300
AlwNI	CAGNNNCTG	3 cut(s)	323, 626, 2435
Ama87I	CYCGRG	1 cut(s)	228
Aor13HI	TCCGGA	3 cut(s)	1898, 2455, 2536
AoxI	GGCC	5 cut(s)	33, 1301, 1798, 1862, 1979
ApeKI	GCWGC	3 cut(s)	83, 2417, 2420
ApoI	RAATTY	9 cut(s)	438, 475, 667, 1343, 1753, 1760, 1894, 2022, 2318
Asp718I	GGTACC	1 cut(s)	1705
AspS9I	GGNCC	9 cut(s)	199, 1244, 1289, 1301, 1366, 1798, 1862, 1915, 1979
AsuC2I	CCSGG	2 cut(s)	680, 1891
AsuII	TTCGAA	2 cut(s)	473, 1757
AvaI	CYCGRG	1 cut(s)	228
AvaII	GGWCC	5 cut(s)	199, 1244, 1289, 1366, 1915
BaeI	ACNNNNGTAYC	2 cut(s)	2263, 2296
BamHI	GGATCC	2 cut(s)	943, 1690
BanI	GGYRCC	1 cut(s)	1705
BanII	GRGCYC	1 cut(s)	831
BauI	CACGAG	1 cut(s)	1910
BbsI	GAAGAC	3 cut(s)	668, 1517, 1670
Bbv12I	GWGCWC	2 cut(s)	831, 2590
BbvCI	CCTCAGC	1 cut(s)	1398
BbvI	GCAGC	3 cut(s)	70, 2429, 2432
BccI	CCATC	7 cut(s)	728, 761, 885, 1550, 1734, 1847, 2463
BceAI	ACGGC	3 cut(s)	1838, 2505, 2539
BcgI	CGANNNNNNTGC	2 cut(s)	1622, 1656
BciT130I	CCWGG	3 cut(s)	1190, 2367, 2505
BclI	TGATCA	1 cut(s)	2281
BcnI	CCSGG	2 cut(s)	680, 1891
BcoDI	GTCTC	5 cut(s)	527, 722, 794, 2426, 2580
BfaI	CTAG	6 cut(s)	152, 405, 459, 836, 1119, 1296
BfmI	CTRYAG	2 cut(s)	1983, 2100
BfuAI	ACCTGC	2 cut(s)	537, 1030
BglI	GCCNNNNNGGC	1 cut(s)	32
BglII	AGATCT	2 cut(s)	446, 968
BisI	GCNGC	3 cut(s)	84, 2418, 2421
BlsI	GCNGC	3 cut(s)	85, 2419, 2422
BmcAI	AGTACT	1 cut(s)	271
Bme1390I	CCNGG	5 cut(s)	680, 1190, 1891, 2367, 2505
Bme18I	GGWCC	5 cut(s)	199, 1244, 1289, 1366, 1915
BmeT110I	CYCGRG	1 cut(s)	228
BmgT120I	GGNCC	9 cut(s)	199, 1244, 1289, 1301, 1366, 1798, 1862, 1915, 1979
BmrFI	CCNGG	5 cut(s)	680, 1190, 1891, 2367, 2505
BmrI	ACTGGG	5 cut(s)	143, 333, 1042, 1543, 2162
BmsI	GCATC	4 cut(s)	613, 1578, 1586, 2594
BmuI	ACTGGG	5 cut(s)	143, 333, 1042, 1543, 2162
BpiI	GAAGAC	3 cut(s)	668, 1517, 1670
Bpu10I	CCTNAGC	1 cut(s)	1398
Bpu14I	TTCGAA	2 cut(s)	473, 1757
BpuMI	CCSGG	2 cut(s)	680, 1891
Bsa29I	ATCGAT	3 cut(s)	307, 853, 1422
BsaBI	GATNNNNATC	1 cut(s)	837
BsaI	GGTCTC	2 cut(s)	722, 794
BsaJI	CCNNGG	1 cut(s)	1189
BsaWI	WCCGGW	3 cut(s)	1898, 2455, 2536
BsaXI	ACNNNNNCTCC	2 cut(s)	153, 183
Bsc4I	CCNNNNNNNGG	8 cut(s)	1298, 1627, 2233, 2388, 2389, 2390, 2459, 2504
Bse3DI	GCAATG	3 cut(s)	1068, 1674, 2187
Bse8I	GATNNNNATC	1 cut(s)	837
BseAI	TCCGGA	3 cut(s)	1898, 2455, 2536
BseBI	CCWGG	3 cut(s)	1190, 2367, 2505
BseCI	ATCGAT	3 cut(s)	307, 853, 1422
BseDI	CCNNGG	1 cut(s)	1189
BseGI	GGATG	4 cut(s)	224, 896, 1858, 2474
BseJI	GATNNNNATC	1 cut(s)	837
BseLI	CCNNNNNNNGG	8 cut(s)	1298, 1627, 2233, 2388, 2389, 2390, 2459, 2504
BseMI	GCAATG	3 cut(s)	1068, 1674, 2187
BseMII	CTCAG	5 cut(s)	419, 522, 1389, 1533, 1946
BseXI	GCAGC	3 cut(s)	70, 2429, 2432
BseYI	CCCAGC	2 cut(s)	493, 1686
BsgI	GTGCAG	2 cut(s)	191, 2436
Bsh1285I	CGRYCG	1 cut(s)	2361
BshFI	GGCC	5 cut(s)	35, 1303, 1800, 1864, 1981
BshNI	GGYRCC	1 cut(s)	1705
BshVI	ATCGAT	3 cut(s)	307, 853, 1422
BsiEI	CGRYCG	1 cut(s)	2361
BsiHKAI	GWGCWC	2 cut(s)	831, 2590
BsiHKCI	CYCGRG	1 cut(s)	228
BsiSI	CCGG	6 cut(s)	543, 680, 1891, 1899, 2456, 2537
BslFI	GGGAC	3 cut(s)	212, 997, 1853
BslI	CCNNNNNNNGG	8 cut(s)	1298, 1627, 2233, 2388, 2389, 2390, 2459, 2504
BsmAI	GTCTC	5 cut(s)	527, 722, 794, 2426, 2580
BsmFI	GGGAC	3 cut(s)	212, 997, 1853
BsmI	GAATGC	3 cut(s)	364, 1204, 1868
BsnI	GGCC	5 cut(s)	35, 1303, 1800, 1864, 1981
Bso31I	GGTCTC	2 cut(s)	722, 794
BsoBI	CYCGRG	1 cut(s)	228
Bsp119I	TTCGAA	2 cut(s)	473, 1757
Bsp1286I	GDGCHC	2 cut(s)	831, 2590
Bsp13I	TCCGGA	3 cut(s)	1898, 2455, 2536
BspANI	GGCC	5 cut(s)	35, 1303, 1800, 1864, 1981
BspCNI	CTCAG	5 cut(s)	420, 523, 1390, 1534, 1947
BspDI	ATCGAT	3 cut(s)	307, 853, 1422
BspEI	TCCGGA	3 cut(s)	1898, 2455, 2536
BspMI	ACCTGC	2 cut(s)	537, 1030
BspPI	GGATC	7 cut(s)	833, 938, 951, 1643, 1685, 1698, 2300
BspQI	GCTCTTC	1 cut(s)	2499
BspT104I	TTCGAA	2 cut(s)	473, 1757
BspT107I	GGYRCC	1 cut(s)	1705
BspTNI	GGTCTC	2 cut(s)	722, 794
BsrBI	CCGCTC	1 cut(s)	707
BsrDI	GCAATG	3 cut(s)	1068, 1674, 2187
BssECI	CCNNGG	1 cut(s)	1189
BssSI	CACGAG	1 cut(s)	1910
Bst2BI	CACGAG	1 cut(s)	1910
Bst2UI	CCWGG	3 cut(s)	1190, 2367, 2505
Bst4CI	ACNGT	4 cut(s)	203, 2123, 2437, 2582
Bst6I	CTCTTC	4 cut(s)	15, 1730, 2343, 2499
BstBI	TTCGAA	2 cut(s)	473, 1757
BstC8I	GCNNGC	6 cut(s)	33, 289, 873, 1144, 1866, 2528
BstENI	CCTNNNNNAGG	3 cut(s)	1296, 2231, 2457
BstF5I	GGATG	4 cut(s)	224, 896, 1858, 2474
BstMAI	GTCTC	5 cut(s)	527, 722, 794, 2426, 2580
BstMCI	CGRYCG	1 cut(s)	2361
BstMWI	GCNNNNNNNGC	4 cut(s)	32, 250, 1475, 2414
BstNI	CCWGG	3 cut(s)	1190, 2367, 2505
BstNSI	RCATGY	1 cut(s)	875
BstSCI	CCNGG	5 cut(s)	678, 1188, 1889, 2365, 2503
BstSFI	CTRYAG	2 cut(s)	1983, 2100
BstV1I	GCAGC	3 cut(s)	70, 2429, 2432
BstV2I	GAAGAC	3 cut(s)	668, 1517, 1670
BstX2I	RGATCY	6 cut(s)	446, 838, 943, 968, 1690, 2305
BstYI	RGATCY	6 cut(s)	446, 838, 943, 968, 1690, 2305
Bsu15I	ATCGAT	3 cut(s)	307, 853, 1422
BsuRI	GGCC	5 cut(s)	35, 1303, 1800, 1864, 1981
BsuTUI	ATCGAT	3 cut(s)	307, 853, 1422
BtsCI	GGATG	4 cut(s)	224, 896, 1858, 2474
BtsIMutI	CAGTG	6 cut(s)	60, 528, 1936, 2128, 2150, 2419
BveI	ACCTGC	2 cut(s)	537, 1030
Cac8I	GCNNGC	6 cut(s)	33, 289, 873, 1144, 1866, 2528
CaiI	CAGNNNCTG	3 cut(s)	323, 626, 2435
Cfr13I	GGNCC	9 cut(s)	199, 1244, 1289, 1301, 1366, 1798, 1862, 1915, 1979
ClaI	ATCGAT	3 cut(s)	307, 853, 1422
CseI	GACGC	1 cut(s)	864
Csp6I	GTAC	6 cut(s)	270, 425, 1706, 2000, 2272, 2563
CspCI	CAANNNNNGTGG	2 cut(s)	496, 531
CviQI	GTAC	6 cut(s)	270, 425, 1706, 2000, 2272, 2563
DraI	TTTAAA	1 cut(s)	1182
Eam1104I	CTCTTC	4 cut(s)	15, 1730, 2343, 2499
EarI	CTCTTC	4 cut(s)	15, 1730, 2343, 2499
EciI	GGCGGA	2 cut(s)	2233, 2439
Ecl136II	GAGCTC	1 cut(s)	829
Eco147I	AGGCCT	1 cut(s)	35
Eco24I	GRGCYC	1 cut(s)	831
Eco31I	GGTCTC	2 cut(s)	722, 794
Eco32I	GATATC	1 cut(s)	1426
Eco47I	GGWCC	5 cut(s)	199, 1244, 1289, 1366, 1915
Eco53kI	GAGCTC	1 cut(s)	829
Eco57I	CTGAAG	4 cut(s)	300, 1932, 2216, 2395
Eco88I	CYCGRG	1 cut(s)	228
EcoICRI	GAGCTC	1 cut(s)	829
EcoNI	CCTNNNNNAGG	3 cut(s)	1296, 2231, 2457
EcoO109I	RGGNCCY	4 cut(s)	1301, 1366, 1798, 1979
EcoRI	GAATTC	2 cut(s)	1753, 1894
EcoRII	CCWGG	3 cut(s)	1188, 2365, 2503
EcoRV	GATATC	1 cut(s)	1426
EcoT22I	ATGCAT	2 cut(s)	371, 1579
EcoT38I	GRGCYC	1 cut(s)	831
FaqI	GGGAC	3 cut(s)	212, 997, 1853
FauI	CCCGC	4 cut(s)	625, 712, 1961, 2542
FauNDI	CATATG	1 cut(s)	844
FbaI	TGATCA	1 cut(s)	2281
FblI	GTMKAC	2 cut(s)	261, 2484
Fnu4HI	GCNGC	3 cut(s)	84, 2418, 2421
FokI	GGATG	4 cut(s)	211, 903, 1865, 2481
FriOI	GRGCYC	1 cut(s)	831
Fsp4HI	GCNGC	3 cut(s)	84, 2418, 2421
FspBI	CTAG	6 cut(s)	152, 405, 459, 836, 1119, 1296
GluI	GCNGC	3 cut(s)	84, 2418, 2421
GsaI	CCCAGC	2 cut(s)	497, 1690
HaeIII	GGCC	5 cut(s)	35, 1303, 1800, 1864, 1981
HapII	CCGG	6 cut(s)	543, 680, 1891, 1899, 2456, 2537
HgaI	GACGC	1 cut(s)	864
HincII	GTYRAC	1 cut(s)	2485
HindII	GTYRAC	1 cut(s)	2485
HindIII	AAGCTT	1 cut(s)	2133
HinfI	GANTC	4 cut(s)	258, 452, 629, 1419
HpaII	CCGG	6 cut(s)	543, 680, 1891, 1899, 2456, 2537
Hpy166II	GTNNAC	5 cut(s)	262, 552, 1915, 2093, 2485
Hpy188I	TCNGA	7 cut(s)	161, 451, 482, 1587, 1852, 1951, 2431
Hpy8I	GTNNAC	5 cut(s)	262, 552, 1915, 2093, 2485
Hpy99I	CGWCG	2 cut(s)	1358, 2525
HpyAV	CCTTC	9 cut(s)	480, 598, 920, 999, 1302, 1811, 1970, 2419, 2453
HpyCH4III	ACNGT	4 cut(s)	203, 2123, 2437, 2582
HpyCH4IV	ACGT	4 cut(s)	1044, 1092, 1099, 1551
HpyF10VI	GCNNNNNNNGC	4 cut(s)	32, 250, 1475, 2414
HpySE526I	ACGT	4 cut(s)	1044, 1092, 1099, 1551
Kpn2I	TCCGGA	3 cut(s)	1898, 2455, 2536
KpnI	GGTACC	1 cut(s)	1709
Ksp22I	TGATCA	1 cut(s)	2281
LguI	GCTCTTC	1 cut(s)	2499
LmnI	GCTCC	5 cut(s)	250, 712, 826, 1174, 2220
Lsp1109I	GCAGC	3 cut(s)	70, 2429, 2432
LweI	GCATC	4 cut(s)	613, 1578, 1586, 2594
MaeI	CTAG	6 cut(s)	152, 405, 459, 836, 1119, 1296
MaeII	ACGT	4 cut(s)	1044, 1092, 1099, 1551
MbiI	CCGCTC	1 cut(s)	707
MfeI	CAATTG	1 cut(s)	1638
MflI	RGATCY	6 cut(s)	446, 838, 943, 968, 1690, 2305
MhlI	GDGCHC	2 cut(s)	831, 2590
MlyI	GAGTC	1 cut(s)	267
MmeI	TCCRAC	3 cut(s)	804, 1152, 1940
Mph1103I	ATGCAT	2 cut(s)	371, 1579
MroI	TCCGGA	3 cut(s)	1898, 2455, 2536
MseI	TTAA	6 cut(s)	849, 1181, 1938, 2016, 2073, 2198
MslI	CAYNNNNRTG	3 cut(s)	1191, 1554, 2583
MspA1I	CMGCKG	6 cut(s)	16, 497, 809, 1313, 1397, 1686
MspI	CCGG	6 cut(s)	543, 680, 1891, 1899, 2456, 2537
MspR9I	CCNGG	5 cut(s)	680, 1190, 1891, 2367, 2505
MunI	CAATTG	1 cut(s)	1638
Mva1269I	GAATGC	3 cut(s)	364, 1204, 1868
MvaI	CCWGG	3 cut(s)	1190, 2367, 2505
MwoI	GCNNNNNNNGC	4 cut(s)	32, 250, 1475, 2414
NciI	CCSGG	2 cut(s)	680, 1891
NdeI	CATATG	1 cut(s)	844
NmeAIII	GCCGAG	1 cut(s)	2037
NmuCI	GTSAC	9 cut(s)	203, 635, 860, 1040, 1093, 1537, 1808, 2123, 2393
NsiI	ATGCAT	2 cut(s)	371, 1579
NspI	RCATGY	1 cut(s)	875
NspV	TTCGAA	2 cut(s)	473, 1757
OliI	CACNNNNGTG	2 cut(s)	1191, 2583
PaeI	GCATGC	1 cut(s)	875
PceI	AGGCCT	1 cut(s)	35
PciSI	GCTCTTC	1 cut(s)	2499
PctI	GAATGC	3 cut(s)	364, 1204, 1868
PfeI	GAWTC	3 cut(s)	452, 629, 1419
PfoI	TCCNGGA	1 cut(s)	1889
PkrI	GCNGC	3 cut(s)	85, 2419, 2422
PleI	GAGTC	1 cut(s)	266
PpsI	GAGTC	1 cut(s)	266
PpuMI	RGGWCCY	1 cut(s)	1366
PsiI	TTATAA	1 cut(s)	1571
Psp124BI	GAGCTC	1 cut(s)	831
Psp5II	RGGWCCY	1 cut(s)	1366
Psp6I	CCWGG	3 cut(s)	1188, 2365, 2503
PspFI	CCCAGC	2 cut(s)	493, 1686
PspGI	CCWGG	3 cut(s)	1188, 2365, 2503
PspPI	GGNCC	9 cut(s)	199, 1244, 1289, 1301, 1366, 1798, 1862, 1915, 1979
PspPPI	RGGWCCY	1 cut(s)	1366
PstNI	CAGNNNCTG	3 cut(s)	323, 626, 2435
PsuI	RGATCY	6 cut(s)	446, 838, 943, 968, 1690, 2305
PvuII	CAGCTG	3 cut(s)	497, 809, 1313
RsaI	GTAC	6 cut(s)	271, 426, 1707, 2001, 2273, 2564
RsaNI	GTAC	6 cut(s)	270, 425, 1706, 2000, 2272, 2563
RseI	CAYNNNNRTG	3 cut(s)	1191, 1554, 2583
SacI	GAGCTC	1 cut(s)	831
SalI	GTCGAC	1 cut(s)	2483
SapI	GCTCTTC	1 cut(s)	2499
SaqAI	TTAA	6 cut(s)	849, 1181, 1938, 2016, 2073, 2198
SatI	GCNGC	3 cut(s)	84, 2418, 2421
Sau96I	GGNCC	9 cut(s)	199, 1244, 1289, 1301, 1366, 1798, 1862, 1915, 1979
ScaI	AGTACT	1 cut(s)	271
SchI	GAGTC	1 cut(s)	267
ScrFI	CCNGG	5 cut(s)	680, 1190, 1891, 2367, 2505
SduI	GDGCHC	2 cut(s)	831, 2590
SfaNI	GCATC	4 cut(s)	613, 1578, 1586, 2594
SfcI	CTRYAG	2 cut(s)	1983, 2100
SfuI	TTCGAA	2 cut(s)	473, 1757
SinI	GGWCC	5 cut(s)	199, 1244, 1289, 1366, 1915
SmiMI	CAYNNNNRTG	3 cut(s)	1191, 1554, 2583
SphI	GCATGC	1 cut(s)	875
SseBI	AGGCCT	1 cut(s)	35
SspI	AATATT	1 cut(s)	379
SspMI	CTAG	6 cut(s)	152, 405, 459, 836, 1119, 1296
SstI	GAGCTC	1 cut(s)	831
StuI	AGGCCT	1 cut(s)	35
StyD4I	CCNGG	5 cut(s)	678, 1188, 1889, 2365, 2503
TaaI	ACNGT	4 cut(s)	203, 2123, 2437, 2582
TaiI	ACGT	4 cut(s)	1047, 1095, 1102, 1554
TaqII	GACCGA	1 cut(s)	2375
TatI	WGTACW	2 cut(s)	269, 1999
TfiI	GAWTC	3 cut(s)	452, 629, 1419
Tru1I	TTAA	6 cut(s)	849, 1181, 1938, 2016, 2073, 2198
Tru9I	TTAA	6 cut(s)	849, 1181, 1938, 2016, 2073, 2198
TscAI	CASTG	6 cut(s)	67, 535, 1936, 2128, 2157, 2419
TseFI	GTSAC	9 cut(s)	203, 635, 860, 1040, 1093, 1537, 1808, 2123, 2393
TseI	GCWGC	3 cut(s)	83, 2417, 2420
Tsp45I	GTSAC	9 cut(s)	203, 635, 860, 1040, 1093, 1537, 1808, 2123, 2393
TspGWI	ACGGA	3 cut(s)	1371, 2298, 2465
TspRI	CASTG	6 cut(s)	67, 535, 1936, 2128, 2157, 2419
VpaK11BI	GGWCC	5 cut(s)	199, 1244, 1289, 1366, 1915
XagI	CCTNNNNNAGG	3 cut(s)	1296, 2231, 2457
XapI	RAATTY	9 cut(s)	438, 475, 667, 1343, 1753, 1760, 1894, 2022, 2318
XbaI	TCTAGA	2 cut(s)	835, 1295
XceI	RCATGY	1 cut(s)	875
XmiI	GTMKAC	2 cut(s)	261, 2484
XspI	CTAG	6 cut(s)	152, 405, 459, 836, 1119, 1296
ZrmI	AGTACT	1 cut(s)	271
Zsp2I	ATGCAT	2 cut(s)	371, 1579

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.