Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0013348

Physical Location & Seq

Genomic Coordinates Forward (+)

53396091 .. 53399647

Gene Structure 3557 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG332800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2613 bp

ATGGCAATGCTACCGCTGTCAATCACACTACAGGCCTGTTGTTGGGCCTGTGGAACCACTGAATCGTCTAAGCTGACAGCCACTACTCATTATAGAGACAGAAACAAAACTACGCTCGGTTTGGTAAAATTGGACGCGGAGAGAAAGCTAGTTTTTGGAGGATTTGTGCAGAGTTTCACACAAAGTTTGCCGCGGGGTTGTCACTCCAGAGTGCAAGCTACATCAGGAGTTTCTATTGAACCAATGGAGCAGAAGTTTCCCACCGGTACTGAAGAAACGGATAAGGTATCAACTTATCTATTTAGGACAGAAAATGGGGATCTGGTGAATGTGTTTGTTAGAAATAATGCTGTCAACTACAGTGTGTATGTTGAAGTTTCATCATTACAATTGTCCAGCAGTGCTGACAGGCTAGTCCTCAGTTGGGGCATGTATAGAGCTGATTCATCCAGTCTCATGCCTTCAGACTTTATCAAAACCCCATTTATAAAGACTTCTTCAGGCAGTTTTACTCTTGAATTGGAGTTTGAGGCTAAACAAACCCCTTTCTATCTCTCATTTATTGTGAAATCTCTGGCAGATGCAAATTTGAGTGGTTTGGAAATAAGAAGCCATAGGAAGACAAATTTCTGTGTCCCAGTTGGTTTCGGTCGAGGTTATCCTACTCCATTGGGTCTTTCCTTTACTAGTGATGGTTCCATAAACTTTGCCATTTTTTCGCGAAATGCAGAAAGTGTGGTTTTATGCTTGTACGATGACCCCGCTGCTCAGAAGCCTGCTCTGGAGCTTGATCTAGATCCATATGTCAATCGATCAGGTGACATATGGCATGTGTCATTTGAAAGTGCATGGACTTTTGTGAGCTATGGCTACAGATTTAAGGGGACCCTTCTGAGAAACACAAATAGCTTTGATGAAGGGGATATTCTTCTGGATCCATATGCTAAGGTCATTGATGAATCTATCCCTAATAATAAAGGAGCAGGGTCGAAGTTAGTTGGAAGACTATGTGAGGAACCTGTGTTTGACTGGGATGGTGATGTTCGACCTCACCTGCCAATAGAGAAACTGATGGTTTATCGGTTGAACCTGACACGTTTTACTGAGCACAAGTCTAGTAAGCTACCTACTAATGTAGCAGGTACGTTTTCTGGTTTGACAGAAAAGTTGGATCAATTTAAAGATCTGGGTGTGAATGCAATTTTGTTAGAGCCAATTTTCCCATTTGATGAGGAAAAAGGTCCATATTTCCCTTGCCATTTTTTTTCACCAATGAATTGTTTTGGACCATCTGGAGGCCCTATAGCAACTATCAACTCGATGAAAGAGATGGTGAAAGAGTTTCATGCCAATGGAATAGAGGTCCTACTGGAAGTTGTTTTCACCCATACTGCTGCGGGTGAAGTGCTGCAAGGAATCGACACCTCATCTTATTATCATGCAAGTACAGCTAAGGATTTGGAAGCAAGAAATTCTTTGAATTGTAACCATCCCATTGTGCAGCAAATGGTATTAGATAGTCTTCGATACTGGGTGACTGAGTTTCACGTCGATGGTTTTTGTTTCATAAATGCATCTTCTCTGTTACGTGGGGTTAACGGTGAATACCAATCTCGCCCGCCTTTGGTTGAAGCAATTGCTTTTGATCCACTACTGACAAAGACCAAGTTCATTGCAGACTGCTGGGACCCTCATGAAATGGTACCAAAGGAAACTCGTTTTCCTCATTGGAAGAGATGGGCAGAAGTGAATGCAAGATTCTGCAATGATGTAAGAAACTTTTTGAGGGGTGAGGGTCTTCTTAGTGACCTTGCTACACGGCTTTGTGGGAACGGGGACATCTTTTCAGATGGGCGAGGCCCAGCATTCTCTTTCAATTTTATTTCCAGAAATTCTGGTCTTCCTCTTGTGGACCTTGTTAGTTTCAGTGGCAGTGAGTTAGCTTCAGAACTGAGTTGGAATTGTGGGGAAGAAGGGCCTACAAGTAAAACTGCTGTACTAGAAAGACGCCTTAAACAAATCCGTAATTTTCTGTTTATCTTGTATCTTTCATTGGGCGTCCCTGTTCTTAACATGGGAGACGAGTGTGGACAATCTTCAGGTGGTTCCCCTGCATACAGTGACAGAAAATCTTTCGACTGGAATGCAATGGAAACAGGTTTTGCTACTCAAACCAGACAGTTTATTGCATATTTAAGTTCATTCAGGAGGAGGCGGAGTGATCTTCTTCAGAATAAGCACTACTTTAAAGAAGAAAATATTGACTGGTATGGGAGTGACCAATCGTTACCGAGGTGGGAAGACCCTCTGTGCAAATTCCTTGCCATGAGGTTGAAGGCAGACCAAGATGAAGTGGAGAACCAATTGACCAACGAATATGCTGATTTAAGGGGTGACTTGTTTGTAGCCTTCAGTGCAGCTGAACAGTCAGAGACTGTTATTCTACCTCCACCCCCAGAAGGTATGGCATGGAGACGTTTGGTTGACACGGCTCTCCCATTTCCTGGATTTTTCTCAATTGATGGTGAGCCTATCATTGAGCAAATGGAGGGTTTATTTTCTTATGCCATGAAGTCTCACAGTTGTGCTCTTTTTGAAGCCAGAAGATTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009251 GO:0009507 GO:0009536 GO:0009987 GO:0010021 GO:0015980 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0032991 GO:0043033 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0071704 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1902494 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

870

Amino Acids

97.59

Weight (kDa)

5.34

Isoelectric Point (pI)

45.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	223 - 315	3e-12	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	379 - 466	6.3e-10	Alpha amylase, catalytic domain
ISOA1-3_C	PF21156	756 - 836	4.3e-06	Isoamylase 1-3, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0013348)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03310 AT1G03310
fragaria_vesca	FvH4_2g23950
malus_domestica	MD03G1268800.v1.1 MD11G1289600.v1.1
prunus_persica	Prupe.8G244800_v2.0.a1
pyrus_communis	pycom11g25700
rosa_chinensis	RchiOBHm_Chr6g0291581
rosa_laevigata	RLG00000012105
rosa_multiflora	Rmu_sc0002226.1_g000007
rosa_roxburghii	Rroxscaffold_7G00175950
rosa_rugosa	Rorug06G0220400 Rorug06G0220400
rosa_samantha	Rh6AG332800 Rh6BG339600 Rh6CG346000 Rh6DG333200
rosa_wichuraiana	Rw6G028900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	488
AarI	CACCTGC	1 cut(s)	1062
AasI	GACNNNNNNGTC	1 cut(s)	413
Acc36I	ACCTGC	2 cut(s)	1062, 1130
Acc65I	GGTACC	1 cut(s)	1702
AccB1I	GGYRCC	1 cut(s)	1702
AccB7I	CCANNNNNTGG	1 cut(s)	2504
AccII	CGCG	3 cut(s)	137, 193, 721
AciI	CCGC	8 cut(s)	14, 137, 191, 193, 762, 1397, 1619, 2215
AclWI	GGATC	6 cut(s)	327, 791, 929, 942, 1179, 1640
AcsI	RAATTY	5 cut(s)	586, 625, 1471, 1891, 2315
AcuI	CTGAAG	7 cut(s)	291, 447, 483, 1929, 2082, 2213, 2395
AcyI	GRCGYC	2 cut(s)	2008, 2058
AfaI	GTAC	6 cut(s)	268, 752, 1144, 1447, 1704, 1998
AfiI	CCNNNNNNNGG	6 cut(s)	42, 424, 1295, 1624, 2459, 2504
AflIII	ACRYGT	1 cut(s)	1094
AgeI	ACCGGT	1 cut(s)	263
AhlI	ACTAGT	1 cut(s)	686
AjiI	CACGTC	1 cut(s)	1549
AjnI	CCWGG	1 cut(s)	2503
AleI	CACNNNNGTG	1 cut(s)	2583
Alw21I	GWGCWC	2 cut(s)	1110, 2590
Alw26I	GTCTC	6 cut(s)	90, 458, 2073, 2426, 2467, 2580
AlwI	GGATC	6 cut(s)	327, 791, 929, 942, 1179, 1640
AlwNI	CAGNNNCTG	1 cut(s)	2435
AoxI	GGCC	5 cut(s)	33, 45, 1297, 1858, 1976
ApeKI	GCWGC	5 cut(s)	764, 1394, 1408, 1501, 2417
ApoI	RAATTY	5 cut(s)	586, 625, 1471, 1891, 2315
AsiGI	ACCGGT	1 cut(s)	263
Asp718I	GGTACC	1 cut(s)	1702
AvaII	GGWCC	6 cut(s)	885, 1241, 1286, 1363, 1687, 1912
BamHI	GGATCC	1 cut(s)	934
BanI	GGYRCC	1 cut(s)	1702
BbsI	GAAGAC	6 cut(s)	626, 1009, 1514, 1790, 1892, 2307
Bbv12I	GWGCWC	2 cut(s)	1110, 2590
BbvI	GCAGC	5 cut(s)	751, 1381, 1395, 1513, 2429
BceAI	ACGGC	2 cut(s)	1835, 2505
BcgI	CGANNNNNNTGC	2 cut(s)	2126, 2160
BciT130I	CCWGG	1 cut(s)	2505
BcoDI	GTCTC	6 cut(s)	90, 458, 2073, 2426, 2467, 2580
BcuI	ACTAGT	1 cut(s)	686
BfaI	CTAG	6 cut(s)	149, 413, 687, 794, 1116, 2000
BfmI	CTRYAG	4 cut(s)	29, 358, 871, 1302
BfuAI	ACCTGC	2 cut(s)	1062, 1130
BglII	AGATCT	1 cut(s)	1183
BisI	GCNGC	6 cut(s)	191, 765, 1395, 1409, 1502, 2418
BlsI	GCNGC	6 cut(s)	192, 766, 1396, 1410, 1503, 2419
Bme1390I	CCNGG	1 cut(s)	2505
Bme18I	GGWCC	6 cut(s)	885, 1241, 1286, 1363, 1687, 1912
BmgBI	CACGTC	1 cut(s)	1549
BmrFI	CCNGG	1 cut(s)	2505
BmrI	ACTGGG	3 cut(s)	632, 1039, 1540
BmsI	GCATC	2 cut(s)	571, 1583
BmuI	ACTGGG	3 cut(s)	632, 1039, 1540
BpiI	GAAGAC	6 cut(s)	626, 1009, 1514, 1790, 1892, 2307
BpmI	CTGGAG	3 cut(s)	190, 803, 1314
Bpu10I	CCTNAGC	2 cut(s)	945, 1452
Bsa29I	ATCGAT	1 cut(s)	811
BsaAI	YACGTR	1 cut(s)	1589
BsaBI	GATNNNNATC	1 cut(s)	795
BsaHI	GRCGYC	2 cut(s)	2008, 2058
BsaJI	CCNNGG	2 cut(s)	191, 2291
BsaWI	WCCGGW	1 cut(s)	263
BsaXI	ACNNNNNCTCC	6 cut(s)	150, 180, 239, 269, 2203, 2233
Bsc4I	CCNNNNNNNGG	6 cut(s)	42, 424, 1295, 1624, 2459, 2504
Bse118I	RCCGGY	1 cut(s)	263
Bse1I	ACTGG	7 cut(s)	450, 638, 1034, 1374, 1535, 2144, 2270
Bse3DI	GCAATG	4 cut(s)	12, 1671, 1771, 2154
Bse8I	GATNNNNATC	1 cut(s)	795
BseBI	CCWGG	1 cut(s)	2505
BseCI	ATCGAT	1 cut(s)	811
BseDI	CCNNGG	2 cut(s)	191, 2291
BseGI	GGATG	3 cut(s)	446, 1039, 1489
BseJI	GATNNNNATC	1 cut(s)	795
BseLI	CCNNNNNNNGG	6 cut(s)	42, 424, 1295, 1624, 2459, 2504
BseMI	GCAATG	4 cut(s)	12, 1671, 1771, 2154
BseMII	CTCAG	6 cut(s)	433, 782, 884, 1095, 1530, 1943
BseNI	ACTGG	7 cut(s)	450, 638, 1034, 1374, 1535, 2144, 2270
BseRI	GAGGAG	1 cut(s)	2224
BseXI	GCAGC	5 cut(s)	751, 1381, 1395, 1513, 2429
BseYI	CCCAGC	2 cut(s)	1683, 1861
BsgI	GTGCAG	3 cut(s)	188, 1520, 2436
Bsh1236I	CGCG	3 cut(s)	137, 193, 721
Bsh1285I	CGRYCG	1 cut(s)	652
BshFI	GGCC	5 cut(s)	35, 47, 1299, 1860, 1978
BshNI	GGYRCC	1 cut(s)	1702
BshTI	ACCGGT	1 cut(s)	263
BshVI	ATCGAT	1 cut(s)	811
BsiEI	CGRYCG	1 cut(s)	652
BsiHKAI	GWGCWC	2 cut(s)	1110, 2590
BsiSI	CCGG	1 cut(s)	264
BslFI	GGGAC	5 cut(s)	620, 898, 1700, 1850, 2045
BslI	CCNNNNNNNGG	6 cut(s)	42, 424, 1295, 1624, 2459, 2504
BsmAI	GTCTC	6 cut(s)	90, 458, 2073, 2426, 2467, 2580
BsmBI	CGTCTC	2 cut(s)	2073, 2467
BsmFI	GGGAC	5 cut(s)	620, 898, 1700, 1850, 2045
BsmI	GAATGC	4 cut(s)	1201, 1756, 1865, 2149
BsnI	GGCC	5 cut(s)	35, 47, 1299, 1860, 1978
Bsp1286I	GDGCHC	2 cut(s)	1110, 2590
Bsp143I	GATC	9 cut(s)	319, 790, 796, 812, 934, 1171, 1183, 1645, 2221
Bsp68I	TCGCGA	1 cut(s)	721
BspACI	CCGC	8 cut(s)	14, 137, 191, 193, 762, 1397, 1619, 2215
BspANI	GGCC	5 cut(s)	35, 47, 1299, 1860, 1978
BspCNI	CTCAG	6 cut(s)	432, 781, 885, 1096, 1531, 1944
BspDI	ATCGAT	1 cut(s)	811
BspFNI	CGCG	3 cut(s)	137, 193, 721
BspHI	TCATGA	1 cut(s)	1693
BspMI	ACCTGC	2 cut(s)	1062, 1130
BspPI	GGATC	6 cut(s)	327, 791, 929, 942, 1179, 1640
BspT107I	GGYRCC	1 cut(s)	1702
BsrDI	GCAATG	4 cut(s)	12, 1671, 1771, 2154
BsrFI	RCCGGY	1 cut(s)	263
BsrI	ACTGG	7 cut(s)	450, 638, 1034, 1374, 1535, 2144, 2270
BssAI	RCCGGY	1 cut(s)	263
BssECI	CCNNGG	2 cut(s)	191, 2291
BssMI	GATC	9 cut(s)	319, 790, 796, 812, 934, 1171, 1183, 1645, 2221
BssNI	GRCGYC	2 cut(s)	2008, 2058
Bst2UI	CCWGG	1 cut(s)	2505
Bst4CI	ACNGT	7 cut(s)	362, 1601, 2120, 2181, 2427, 2437, 2582
Bst6I	CTCTTC	1 cut(s)	1727
BstACI	GRCGYC	2 cut(s)	2008, 2058
BstBAI	YACGTR	1 cut(s)	1589
BstC8I	GCNNGC	3 cut(s)	216, 777, 1619
BstDSI	CCRYGG	1 cut(s)	191
BstF5I	GGATG	3 cut(s)	446, 1039, 1489
BstFNI	CGCG	3 cut(s)	137, 193, 721
BstKTI	GATC	9 cut(s)	322, 793, 799, 815, 937, 1174, 1186, 1648, 2224
BstMAI	GTCTC	6 cut(s)	90, 458, 2073, 2426, 2467, 2580
BstMBI	GATC	9 cut(s)	319, 790, 796, 812, 934, 1171, 1183, 1645, 2221
BstMCI	CGRYCG	1 cut(s)	652
BstMWI	GCNNNNNNNGC	2 cut(s)	1448, 2414
BstNI	CCWGG	1 cut(s)	2505
BstNSI	RCATGY	2 cut(s)	433, 833
BstSCI	CCNGG	1 cut(s)	2503
BstSFI	CTRYAG	4 cut(s)	29, 358, 871, 1302
BstUI	CGCG	3 cut(s)	137, 193, 721
BstV1I	GCAGC	5 cut(s)	751, 1381, 1395, 1513, 2429
BstV2I	GAAGAC	6 cut(s)	626, 1009, 1514, 1790, 1892, 2307
BstX2I	RGATCY	4 cut(s)	319, 796, 934, 1183
BstYI	RGATCY	4 cut(s)	319, 796, 934, 1183
Bsu15I	ATCGAT	1 cut(s)	811
BsuRI	GGCC	5 cut(s)	35, 47, 1299, 1860, 1978
BsuTUI	ATCGAT	1 cut(s)	811
BtgI	CCRYGG	1 cut(s)	191
BtrI	CACGTC	1 cut(s)	1549
BtsCI	GGATG	3 cut(s)	446, 1039, 1489
BtsI	GCAGTG	2 cut(s)	406, 1939
BtsIMutI	CAGTG	7 cut(s)	57, 367, 406, 1933, 1939, 2125, 2419
BtuMI	TCGCGA	1 cut(s)	721
BveI	ACCTGC	2 cut(s)	1062, 1130
Cac8I	GCNNGC	3 cut(s)	216, 777, 1619
CaiI	CAGNNNCTG	1 cut(s)	2435
CciI	TCATGA	1 cut(s)	1693
Cfr10I	RCCGGY	1 cut(s)	263
Cfr42I	CCGCGG	1 cut(s)	194
ClaI	ATCGAT	1 cut(s)	811
CseI	GACGC	3 cut(s)	143, 2016, 2047
Csp6I	GTAC	6 cut(s)	267, 751, 1143, 1446, 1703, 1997
CspAI	ACCGGT	1 cut(s)	263
CviQI	GTAC	6 cut(s)	267, 751, 1143, 1446, 1703, 1997
DpnI	GATC	9 cut(s)	321, 792, 798, 814, 936, 1173, 1185, 1647, 2223
DpnII	GATC	9 cut(s)	319, 790, 796, 812, 934, 1171, 1183, 1645, 2221
DraI	TTTAAA	2 cut(s)	1180, 2248
DrdI	GACNNNNNNGTC	1 cut(s)	413
DseDI	GACNNNNNNGTC	1 cut(s)	413
Eam1104I	CTCTTC	1 cut(s)	1727
EarI	CTCTTC	1 cut(s)	1727
EciI	GGCGGA	1 cut(s)	2230
Eco147I	AGGCCT	1 cut(s)	35
Eco47I	GGWCC	6 cut(s)	885, 1241, 1286, 1363, 1687, 1912
Eco57I	CTGAAG	7 cut(s)	291, 447, 483, 1929, 2082, 2213, 2395
EcoO109I	RGGNCCY	5 cut(s)	885, 1298, 1363, 1687, 1976
EcoRII	CCWGG	1 cut(s)	2503
EcoT22I	ATGCAT	1 cut(s)	1576
Esp3I	CGTCTC	2 cut(s)	2073, 2467
FalI	AAGNNNNNCTT	4 cut(s)	1459, 1491, 2228, 2260
FaqI	GGGAC	5 cut(s)	620, 898, 1700, 1850, 2045
FauI	CCCGC	4 cut(s)	186, 769, 1390, 1626
FauNDI	CATATG	3 cut(s)	802, 824, 940
Fnu4HI	GCNGC	6 cut(s)	191, 765, 1395, 1409, 1502, 2418
FokI	GGATG	3 cut(s)	433, 1046, 1476
Fsp4HI	GCNGC	6 cut(s)	191, 765, 1395, 1409, 1502, 2418
FspBI	CTAG	6 cut(s)	149, 413, 687, 794, 1116, 2000
GluI	GCNGC	6 cut(s)	191, 765, 1395, 1409, 1502, 2418
GsaI	CCCAGC	2 cut(s)	1687, 1865
GsuI	CTGGAG	3 cut(s)	190, 803, 1314
HaeIII	GGCC	5 cut(s)	35, 47, 1299, 1860, 1978
HapII	CCGG	1 cut(s)	264
HgaI	GACGC	3 cut(s)	143, 2016, 2047
Hin1I	GRCGYC	2 cut(s)	2008, 2058
HincII	GTYRAC	3 cut(s)	355, 1597, 2485
HindII	GTYRAC	3 cut(s)	355, 1597, 2485
HinfI	GANTC	5 cut(s)	62, 443, 959, 1416, 1758
HpaI	GTTAAC	1 cut(s)	1597
HpaII	CCGG	1 cut(s)	264
Hpy166II	GTNNAC	5 cut(s)	355, 1597, 1912, 2090, 2485
Hpy188I	TCNGA	7 cut(s)	466, 771, 894, 1849, 1948, 2232, 2431
Hpy8I	GTNNAC	5 cut(s)	355, 1597, 1912, 2090, 2485
Hpy99I	CGWCG	1 cut(s)	1553
HpyAV	CCTTC	7 cut(s)	471, 899, 911, 1967, 2329, 2419, 2453
HpyCH4III	ACNGT	7 cut(s)	362, 1601, 2120, 2181, 2427, 2437, 2582
HpyCH4IV	ACGT	5 cut(s)	1096, 1145, 1548, 1588, 2476
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1448, 2414
HpySE526I	ACGT	5 cut(s)	1096, 1145, 1548, 1588, 2476
Hsp92I	GRCGYC	2 cut(s)	2008, 2058
KflI	GGGWCCC	2 cut(s)	885, 1687
KpnI	GGTACC	1 cut(s)	1706
KspAI	GTTAAC	1 cut(s)	1597
KspI	CCGCGG	1 cut(s)	194
Kzo9I	GATC	9 cut(s)	319, 790, 796, 812, 934, 1171, 1183, 1645, 2221
LmnI	GCTCC	3 cut(s)	247, 784, 980
Lsp1109I	GCAGC	5 cut(s)	751, 1381, 1395, 1513, 2429
LweI	GCATC	2 cut(s)	571, 1583
MaeI	CTAG	6 cut(s)	149, 413, 687, 794, 1116, 2000
MaeII	ACGT	5 cut(s)	1096, 1145, 1548, 1588, 2476
MalI	GATC	9 cut(s)	321, 792, 798, 814, 936, 1173, 1185, 1647, 2223
MboI	GATC	9 cut(s)	319, 790, 796, 812, 934, 1171, 1183, 1645, 2221
MfeI	CAATTG	4 cut(s)	389, 1635, 2363, 2517
MflI	RGATCY	4 cut(s)	319, 796, 934, 1183
MhlI	GDGCHC	2 cut(s)	1110, 2590
MmeI	TCCRAC	3 cut(s)	979, 1149, 1937
Mph1103I	ATGCAT	1 cut(s)	1576
MseI	TTAA	8 cut(s)	879, 1179, 1596, 2013, 2070, 2195, 2247, 2387
MslI	CAYNNNNRTG	3 cut(s)	1350, 1551, 2583
MspA1I	CMGCKG	4 cut(s)	16, 193, 764, 2420
MspI	CCGG	1 cut(s)	264
MspR9I	CCNGG	1 cut(s)	2505
MunI	CAATTG	4 cut(s)	389, 1635, 2363, 2517
Mva1269I	GAATGC	4 cut(s)	1201, 1756, 1865, 2149
MvaI	CCWGG	1 cut(s)	2505
MvnI	CGCG	3 cut(s)	137, 193, 721
MwoI	GCNNNNNNNGC	2 cut(s)	1448, 2414
NdeI	CATATG	3 cut(s)	802, 824, 940
NdeII	GATC	9 cut(s)	319, 790, 796, 812, 934, 1171, 1183, 1645, 2221
NmuCI	GTSAC	7 cut(s)	200, 818, 1534, 1805, 2120, 2276, 2393
NruI	TCGCGA	1 cut(s)	721
NsiI	ATGCAT	1 cut(s)	1576
NspI	RCATGY	2 cut(s)	433, 833
OliI	CACNNNNGTG	1 cut(s)	2583
PagI	TCATGA	1 cut(s)	1693
PaqCI	CACCTGC	1 cut(s)	1062
PceI	AGGCCT	1 cut(s)	35
PctI	GAATGC	4 cut(s)	1201, 1756, 1865, 2149
PfeI	GAWTC	5 cut(s)	62, 443, 959, 1416, 1758
PflMI	CCANNNNNTGG	1 cut(s)	2504
PfoI	TCCNGGA	1 cut(s)	2503
PinAI	ACCGGT	1 cut(s)	263
PkrI	GCNGC	6 cut(s)	192, 766, 1396, 1410, 1503, 2419
Ppu21I	YACGTR	1 cut(s)	1589
PpuMI	RGGWCCY	3 cut(s)	885, 1363, 1687
PsiI	TTATAA	1 cut(s)	488
Psp5II	RGGWCCY	3 cut(s)	885, 1363, 1687
Psp6I	CCWGG	1 cut(s)	2503
PspFI	CCCAGC	2 cut(s)	1683, 1861
PspGI	CCWGG	1 cut(s)	2503
PspPPI	RGGWCCY	3 cut(s)	885, 1363, 1687
PstNI	CAGNNNCTG	1 cut(s)	2435
PsuI	RGATCY	4 cut(s)	319, 796, 934, 1183
PvuII	CAGCTG	1 cut(s)	2420
RruI	TCGCGA	1 cut(s)	721
RsaI	GTAC	6 cut(s)	268, 752, 1144, 1447, 1704, 1998
RsaNI	GTAC	6 cut(s)	267, 751, 1143, 1446, 1703, 1997
RseI	CAYNNNNRTG	3 cut(s)	1350, 1551, 2583
SacII	CCGCGG	1 cut(s)	194
SaqAI	TTAA	8 cut(s)	879, 1179, 1596, 2013, 2070, 2195, 2247, 2387
SatI	GCNGC	6 cut(s)	191, 765, 1395, 1409, 1502, 2418
Sau3AI	GATC	9 cut(s)	319, 790, 796, 812, 934, 1171, 1183, 1645, 2221
ScrFI	CCNGG	1 cut(s)	2505
SduI	GDGCHC	2 cut(s)	1110, 2590
SfaNI	GCATC	2 cut(s)	571, 1583
SfcI	CTRYAG	4 cut(s)	29, 358, 871, 1302
Sfr303I	CCGCGG	1 cut(s)	194
SgrBI	CCGCGG	1 cut(s)	194
SinI	GGWCC	6 cut(s)	885, 1241, 1286, 1363, 1687, 1912
SmiMI	CAYNNNNRTG	3 cut(s)	1350, 1551, 2583
SpeI	ACTAGT	1 cut(s)	686
SseBI	AGGCCT	1 cut(s)	35
SsiI	CCGC	8 cut(s)	14, 137, 191, 193, 762, 1397, 1619, 2215
SspI	AATATT	1 cut(s)	2260
SspMI	CTAG	6 cut(s)	149, 413, 687, 794, 1116, 2000
StuI	AGGCCT	1 cut(s)	35
StyD4I	CCNGG	1 cut(s)	2503
TaaI	ACNGT	7 cut(s)	362, 1601, 2120, 2181, 2427, 2437, 2582
TaiI	ACGT	5 cut(s)	1099, 1148, 1551, 1591, 2479
TaqI	TCGA	9 cut(s)	652, 811, 989, 1045, 1319, 1419, 1525, 1551, 2136
TaqII	GACCGA	1 cut(s)	638
TatI	WGTACW	2 cut(s)	1445, 1996
TauI	GCSGC	1 cut(s)	193
TfiI	GAWTC	5 cut(s)	62, 443, 959, 1416, 1758
Tru1I	TTAA	8 cut(s)	879, 1179, 1596, 2013, 2070, 2195, 2247, 2387
Tru9I	TTAA	8 cut(s)	879, 1179, 1596, 2013, 2070, 2195, 2247, 2387
TscAI	CASTG	7 cut(s)	64, 367, 406, 1933, 1939, 2125, 2419
TseFI	GTSAC	7 cut(s)	200, 818, 1534, 1805, 2120, 2276, 2393
TseI	GCWGC	5 cut(s)	764, 1394, 1408, 1501, 2417
Tsp45I	GTSAC	7 cut(s)	200, 818, 1534, 1805, 2120, 2276, 2393
TspGWI	ACGGA	2 cut(s)	293, 2012
TspRI	CASTG	7 cut(s)	64, 367, 406, 1933, 1939, 2125, 2419
Van91I	CCANNNNNTGG	1 cut(s)	2504
VpaK11BI	GGWCC	6 cut(s)	885, 1241, 1286, 1363, 1687, 1912
XapI	RAATTY	5 cut(s)	586, 625, 1471, 1891, 2315
XbaI	TCTAGA	1 cut(s)	793
XceI	RCATGY	2 cut(s)	433, 833
XspI	CTAG	6 cut(s)	149, 413, 687, 794, 1116, 2000
Zsp2I	ATGCAT	1 cut(s)	1576

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.