MD03G1269900.v1.1

chloroplast RF68

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0020370
Physical Location & Seq
Forward (+)
35401953 .. 35402380
428 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1269900.v1.1.491

Sequence Viewer

Length: 261 bp
ATGGAGGATCTAACAACGTATCTTCATAGACCAAGAACTACAACATCGCCCCTTTCATTCTTGGGTGACAGACGAATCGTACCATTCGAGTCTGTTTTTCATGCTTTTCCCGGAGGTCTGGAGAAAGCTGCAATCCCACTTGTTCAACTGTTGCTGAGCAATTTTTGGATCAACAGACCTCCCCAAAAGGTAATTTTAATGTGGCCAATTTCTTCTCTCTTTTGCAATCAGTTTTGCAACAGCACATGCTGCTTTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2647

Protein Analysis

87

Amino Acids

9.85

Weight (kDa)

8.49

Isoelectric Point (pI)

40.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020370)

Species Orthologous Gene IDs
malus_domestica MD00G1140100.v1.1 MD03G1269900.v1.1
rosa_chinensis RchiOBHm_Chr2g0095251 RchiOBHm_Chr4g0392721 RchiOBHm_Chr7g0221131
rosa_samantha Rh4CG047300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 15, 176
AcoI YGGCCR 1 cut(s) 203
AfaI GTAC 1 cut(s) 81
AgsI TTSAA 1 cut(s) 146
AluBI AGCT 2 cut(s) 128, 258
AluI AGCT 2 cut(s) 128, 258
AlwI GGATC 2 cut(s) 15, 176
AoxI GGCC 1 cut(s) 203
ApeKI GCWGC 2 cut(s) 128, 249
AsuC2I CCSGG 1 cut(s) 111
AsuHPI GGTGA 1 cut(s) 77
BalI TGGCCA 1 cut(s) 205
BbvI GCAGC 2 cut(s) 115, 236
BcnI CCSGG 1 cut(s) 111
BisI GCNGC 2 cut(s) 129, 250
BlpI GCTNAGC 1 cut(s) 155
BlsI GCNGC 2 cut(s) 130, 251
Bme1390I CCNGG 1 cut(s) 111
BmrFI CCNGG 1 cut(s) 111
BpmI CTGGAG 1 cut(s) 140
Bpu1102I GCTNAGC 1 cut(s) 155
BpuMI CCSGG 1 cut(s) 111
BseMII CTCAG 1 cut(s) 146
BseXI GCAGC 2 cut(s) 115, 236
BshFI GGCC 1 cut(s) 205
BsiSI CCGG 1 cut(s) 111
BsnI GGCC 1 cut(s) 205
Bsp143I GATC 2 cut(s) 7, 168
Bsp1720I GCTNAGC 1 cut(s) 155
BspANI GGCC 1 cut(s) 205
BspCNI CTCAG 1 cut(s) 147
BspPI GGATC 2 cut(s) 15, 176
BssMI GATC 2 cut(s) 7, 168
Bst4CI ACNGT 1 cut(s) 150
BstAPI GCANNNNNTGC 1 cut(s) 249
BstDEI CTNAG 1 cut(s) 155
BstKTI GATC 2 cut(s) 10, 171
BstMBI GATC 2 cut(s) 7, 168
BstMWI GCNNNNNNNGC 2 cut(s) 249, 255
BstNSI RCATGY 1 cut(s) 249
BstSCI CCNGG 1 cut(s) 109
BstV1I GCAGC 2 cut(s) 115, 236
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BsuRI GGCC 1 cut(s) 205
BtgZI GCGATG 1 cut(s) 30
Csp6I GTAC 1 cut(s) 80
CviAII CATG 2 cut(s) 101, 246
CviJI RGCY 3 cut(s) 128, 205, 258
CviKI_1 RGCY 3 cut(s) 128, 205, 258
CviQI GTAC 1 cut(s) 80
DdeI CTNAG 1 cut(s) 155
DpnI GATC 2 cut(s) 9, 170
DpnII GATC 2 cut(s) 7, 168
EaeI YGGCCR 1 cut(s) 203
FaeI CATG 2 cut(s) 104, 249
FaiI YATR 3 cut(s) 27, 102, 247
FatI CATG 2 cut(s) 100, 245
Fnu4HI GCNGC 2 cut(s) 129, 250
Fsp4HI GCNGC 2 cut(s) 129, 250
GluI GCNGC 2 cut(s) 129, 250
GsuI CTGGAG 1 cut(s) 140
HaeIII GGCC 1 cut(s) 205
HapII CCGG 1 cut(s) 111
Hin1II CATG 2 cut(s) 104, 249
HinfI GANTC 2 cut(s) 75, 89
HpaII CCGG 1 cut(s) 111
HphI GGTGA 1 cut(s) 77
Hpy188III TCNNGA 1 cut(s) 119
HpyCH4III ACNGT 1 cut(s) 150
HpyCH4IV ACGT 1 cut(s) 17
HpyCH4V TGCA 3 cut(s) 131, 225, 237
HpyF10VI GCNNNNNNNGC 2 cut(s) 249, 255
HpyF3I CTNAG 1 cut(s) 155
HpySE526I ACGT 1 cut(s) 17
Hsp92II CATG 2 cut(s) 104, 249
Kzo9I GATC 2 cut(s) 7, 168
LpnPI CCDG 2 cut(s) 104, 124
Lsp1109I GCAGC 2 cut(s) 115, 236
MaeII ACGT 1 cut(s) 17
MaeIII GTNAC 1 cut(s) 65
MalI GATC 2 cut(s) 9, 170
MboI GATC 2 cut(s) 7, 168
MboII GAAGA 2 cut(s) 14, 204
MflI RGATCY 1 cut(s) 7
MlsI TGGCCA 1 cut(s) 205
MluCI AATT 3 cut(s) 160, 192, 207
MluNI TGGCCA 1 cut(s) 205
MlyI GAGTC 1 cut(s) 98
MnlI CCTC 2 cut(s) 107, 189
Mox20I TGGCCA 1 cut(s) 205
MscI TGGCCA 1 cut(s) 205
MseI TTAA 1 cut(s) 197
Msp20I TGGCCA 1 cut(s) 205
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 1 cut(s) 111
MwoI GCNNNNNNNGC 2 cut(s) 249, 255
NciI CCSGG 1 cut(s) 111
NdeII GATC 2 cut(s) 7, 168
NlaIII CATG 2 cut(s) 104, 249
NmuCI GTSAC 1 cut(s) 65
NspI RCATGY 1 cut(s) 249
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PfeI GAWTC 1 cut(s) 75
PfoI TCCNGGA 1 cut(s) 109
PkrI GCNGC 2 cut(s) 130, 251
PleI GAGTC 1 cut(s) 97
PpsI GAGTC 1 cut(s) 97
PsuI RGATCY 1 cut(s) 7
RsaI GTAC 1 cut(s) 81
RsaNI GTAC 1 cut(s) 80
SaqAI TTAA 1 cut(s) 197
SatI GCNGC 2 cut(s) 129, 250
Sau3AI GATC 2 cut(s) 7, 168
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 1 cut(s) 111
SetI ASST 6 cut(s) 20, 118, 130, 181, 192, 260
SgeI CNNG 8 cut(s) 45, 73, 100, 113, 122, 123, 131, 152
Sse9I AATT 3 cut(s) 160, 192, 207
StyD4I CCNGG 1 cut(s) 109
TaaI ACNGT 1 cut(s) 150
TaiI ACGT 1 cut(s) 20
TaqI TCGA 1 cut(s) 87
TasI AATT 3 cut(s) 160, 192, 207
TfiI GAWTC 1 cut(s) 75
Tru1I TTAA 1 cut(s) 197
Tru9I TTAA 1 cut(s) 197
TseFI GTSAC 1 cut(s) 65
TseI GCWGC 2 cut(s) 128, 249
Tsp45I GTSAC 1 cut(s) 65
TspDTI ATGAA 3 cut(s) 14, 45, 89
XceI RCATGY 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.