Rh4CG047300

chloroplast RF68

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0020370
Physical Location & Seq
Forward (+)
9464740 .. 9465105
366 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG047300.1

Sequence Viewer

Length: 366 bp
ATGGAGCACCTAACAACGTATCTTCACAGATCAAGAACTACGAGATCGTCACTTTCATTCTGGGGTGACGGAAGGATCGTACCATTTGAGCCTTTTTTTTTTCATGCTTTTCCCGGAGGTCTGGAGAAAGCTGCAATCAATAGGATTTTCCTAATCCTCCCTTCCGGAAAGGAAGATAGTGAAATTGTTTTTCCTTTCCGCATGGACCAGGAGATTGGATCTAGCTGTAAGAAGAATGTTTGGCTGATAAATAACTCACCTCACTTCTTGGTCTTTGACCCCCTCAGTCACTATGAACGCCCCCGATCAGTGCAATGGGATGTGTCTATTTATCTATCTCTTGACTCGAAATCGGAGCAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2647

Protein Analysis

121

Amino Acids

14.08

Weight (kDa)

6.5

Isoelectric Point (pI)

64.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020370)

Species Orthologous Gene IDs
malus_domestica MD00G1140100.v1.1 MD03G1269900.v1.1
rosa_chinensis RchiOBHm_Chr2g0095251 RchiOBHm_Chr4g0392721 RchiOBHm_Chr7g0221131
rosa_samantha Rh4CG047300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 349
AccIII TCCGGA 1 cut(s) 164
AciI CCGC 1 cut(s) 199
AclWI GGATC 2 cut(s) 83, 226
AfaI GTAC 1 cut(s) 81
AjnI CCWGG 1 cut(s) 207
AluBI AGCT 2 cut(s) 131, 225
AluI AGCT 2 cut(s) 131, 225
Alw21I GWGCWC 1 cut(s) 9
AlwI GGATC 2 cut(s) 83, 226
Aor13HI TCCGGA 1 cut(s) 164
ApeKI GCWGC 1 cut(s) 131
AspS9I GGNCC 1 cut(s) 205
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 2 cut(s) 77, 249
AvaII GGWCC 1 cut(s) 205
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 1 cut(s) 118
BciT130I CCWGG 1 cut(s) 209
BcnI CCSGG 1 cut(s) 114
BfaI CTAG 1 cut(s) 222
BfuAI ACCTGC 1 cut(s) 349
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
Bme1390I CCNGG 2 cut(s) 114, 209
Bme18I GGWCC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 205
BmrFI CCNGG 2 cut(s) 114, 209
BpmI CTGGAG 1 cut(s) 143
BpuMI CCSGG 1 cut(s) 114
BsaWI WCCGGW 1 cut(s) 164
Bse3DI GCAATG 1 cut(s) 320
BseAI TCCGGA 1 cut(s) 164
BseBI CCWGG 1 cut(s) 209
BseGI GGATG 1 cut(s) 325
BseMI GCAATG 1 cut(s) 320
BseMII CTCAG 1 cut(s) 298
BseXI GCAGC 1 cut(s) 118
BsiHKAI GWGCWC 1 cut(s) 9
BsiSI CCGG 2 cut(s) 114, 165
Bsp1286I GDGCHC 1 cut(s) 9
Bsp13I TCCGGA 1 cut(s) 164
Bsp143I GATC 5 cut(s) 29, 44, 75, 218, 305
BspACI CCGC 1 cut(s) 199
BspCNI CTCAG 1 cut(s) 297
BspEI TCCGGA 1 cut(s) 164
BspMI ACCTGC 1 cut(s) 349
BspPI GGATC 2 cut(s) 83, 226
BsrDI GCAATG 1 cut(s) 320
BssMI GATC 5 cut(s) 29, 44, 75, 218, 305
Bst2UI CCWGG 1 cut(s) 209
BstDEI CTNAG 1 cut(s) 284
BstF5I GGATG 1 cut(s) 325
BstKTI GATC 5 cut(s) 32, 47, 78, 221, 308
BstMBI GATC 5 cut(s) 29, 44, 75, 218, 305
BstNI CCWGG 1 cut(s) 209
BstSCI CCNGG 2 cut(s) 112, 207
BstV1I GCAGC 1 cut(s) 118
BstX2I RGATCY 1 cut(s) 218
BstXI CCANNNNNNTGG 1 cut(s) 215
BstYI RGATCY 1 cut(s) 218
BtsCI GGATG 1 cut(s) 325
BtsIMutI CAGTG 1 cut(s) 315
BveI ACCTGC 1 cut(s) 349
Cfr13I GGNCC 1 cut(s) 205
Csp6I GTAC 1 cut(s) 80
CviAII CATG 2 cut(s) 104, 202
CviJI RGCY 4 cut(s) 91, 131, 225, 244
CviKI_1 RGCY 4 cut(s) 91, 131, 225, 244
CviQI GTAC 1 cut(s) 80
DdeI CTNAG 1 cut(s) 284
DpnI GATC 5 cut(s) 31, 46, 77, 220, 307
DpnII GATC 5 cut(s) 29, 44, 75, 218, 305
Eco47I GGWCC 1 cut(s) 205
EcoRII CCWGG 1 cut(s) 207
FaeI CATG 2 cut(s) 107, 205
FaiI YATR 3 cut(s) 105, 203, 294
FatI CATG 2 cut(s) 103, 201
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 1 cut(s) 332
Fsp4HI GCNGC 1 cut(s) 132
FspBI CTAG 1 cut(s) 222
GluI GCNGC 1 cut(s) 132
GsuI CTGGAG 1 cut(s) 143
HapII CCGG 2 cut(s) 114, 165
Hin1II CATG 2 cut(s) 107, 205
HinfI GANTC 1 cut(s) 344
HpaII CCGG 2 cut(s) 114, 165
HphI GGTGA 2 cut(s) 77, 249
Hpy188I TCNGA 1 cut(s) 355
Hpy188III TCNNGA 4 cut(s) 33, 122, 165, 341
HpyAV CCTTC 2 cut(s) 66, 171
HpyCH4IV ACGT 1 cut(s) 17
HpyCH4V TGCA 2 cut(s) 134, 313
HpyF3I CTNAG 1 cut(s) 284
HpySE526I ACGT 1 cut(s) 17
Hsp92II CATG 2 cut(s) 107, 205
Kpn2I TCCGGA 1 cut(s) 164
Kzo9I GATC 5 cut(s) 29, 44, 75, 218, 305
LmnI GCTCC 2 cut(s) 4, 355
LpnPI CCDG 7 cut(s) 46, 107, 127, 178, 194, 221, 344
Lsp1109I GCAGC 1 cut(s) 118
MaeI CTAG 1 cut(s) 222
MaeII ACGT 1 cut(s) 17
MaeIII GTNAC 3 cut(s) 48, 65, 287
MalI GATC 5 cut(s) 31, 46, 77, 220, 307
MboI GATC 5 cut(s) 29, 44, 75, 218, 305
MboII GAAGA 3 cut(s) 14, 185, 244
MflI RGATCY 1 cut(s) 218
MhlI GDGCHC 1 cut(s) 9
MluCI AATT 1 cut(s) 183
MlyI GAGTC 1 cut(s) 338
MnlI CCTC 4 cut(s) 110, 167, 270, 293
MroI TCCGGA 1 cut(s) 164
MspI CCGG 2 cut(s) 114, 165
MspR9I CCNGG 2 cut(s) 114, 209
MvaI CCWGG 1 cut(s) 209
NciI CCSGG 1 cut(s) 114
NdeII GATC 5 cut(s) 29, 44, 75, 218, 305
NlaIII CATG 2 cut(s) 107, 205
NmuCI GTSAC 3 cut(s) 48, 65, 287
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PfoI TCCNGGA 1 cut(s) 112
PkrI GCNGC 1 cut(s) 133
PleI GAGTC 1 cut(s) 338
PpsI GAGTC 1 cut(s) 338
Psp6I CCWGG 1 cut(s) 207
PspGI CCWGG 1 cut(s) 207
PspPI GGNCC 1 cut(s) 205
PsuI RGATCY 1 cut(s) 218
RsaI GTAC 1 cut(s) 81
RsaNI GTAC 1 cut(s) 80
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 5 cut(s) 29, 44, 75, 218, 305
Sau96I GGNCC 1 cut(s) 205
SchI GAGTC 1 cut(s) 338
ScrFI CCNGG 2 cut(s) 114, 209
SduI GDGCHC 1 cut(s) 9
SetI ASST 7 cut(s) 12, 20, 121, 133, 227, 262, 363
SinI GGWCC 1 cut(s) 205
Sse9I AATT 1 cut(s) 183
SsiI CCGC 1 cut(s) 199
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 2 cut(s) 112, 207
TaiI ACGT 1 cut(s) 20
TaqI TCGA 1 cut(s) 347
TasI AATT 1 cut(s) 183
TscAI CASTG 1 cut(s) 315
TseFI GTSAC 3 cut(s) 48, 65, 287
TseI GCWGC 1 cut(s) 131
Tsp45I GTSAC 3 cut(s) 48, 65, 287
TspDTI ATGAA 3 cut(s) 45, 92, 309
TspGWI ACGGA 1 cut(s) 84
TspRI CASTG 1 cut(s) 315
VpaK11BI GGWCC 1 cut(s) 205
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.