Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0014575

Physical Location & Seq

Genomic Coordinates Forward (+)

36166350 .. 36170436

Gene Structure 4087 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1280500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1251 bp

ATGGCTGCAATGAGTGGCCTCGCTACGAGCTCCACTCCTTCTCGATTACTCGCATTTCCAATAAGACGCGGTTTCCCGGACCACTCGTCTTCGCGCTCCGTGGTCATCAGTAAACCTCCTCCCAGAGCTCCCAGACTCGTTTGCATGGCGGAGCCCTATTTGATTACAAAGCTGGATTCTGCAGAGAAGACCTGGAAAGATTTATCGGTCAAGCTGGCTGATCCAGACGTTGTGTCAAATCCCAGTGAATACCAGAAGCTTGCGCAGTCTGTGGCAGAGCTTGATGAGGTCGTGACAATCTATAGGGATTTCAAAGACTCCGAGAAGCAGTTAGAAGAGACTAAAGCTTTAGCAAAAGAAGCTGGCAATGATGAGGATATGGCAGAGATGATATCTTTTGAACTTGAATCTTTATCCAATCAAATCAAAGAACTTGAAGCGAAGCTCAAGATGCTACTCCTTCCTAGTGACCCTCTTGATGCAAGAAATATAATGCTTGAAGTAAGGGCTGGTACTGGTGGTGATGAAGCTGGAATCTGGGCAGGTGATCTTGTCCGTATGTATGAGAAGTACAGTGAGAGGAATAATTGGAGGTTCAGCACAGTCTCAAGCTCAGAGGCTGAAAGAGGAGGATACAAGGCCTATGTGATGGAAGTCAAAGGAAAACGTGTATACAGCAAATTAAAATATGAATCAGGTGTTCATCGGGTTCAACGTGTTCCTCATACAGAAACACAGGGTCGCGTGCATACTTCCACTGCGACTGTTGCGATCATGCCAGAGGCGGATGAAGTTGAGGTGGTTATCGACCCAAAAGATATTGAACTGACAACAGCAAGGTCTGGGGGAGCCGGAGGGCAAAATGTCAACAAGGTGGAGACAGCTGTTGATCTTGTCCATAAACCAACGGGCATCCGCATCTTTTGTAGTGAAGAAAGGTCTCAACTTCAGAACAAGCATCGTGCTTTTCAGTTGCTGCGGGCAAAATTGTATAAGATAAAAGTGAGAGAGCAGCAGGAGTTGATTAGGAATCAGCGGAAATCGCAGGTTGGTACTGGTTCTCGTGCAGAAAAGATTCGGACATACAACTTTAAGGACAATAGAGTGACCGACCATCGGTTAAAGACAAACTATGAGCTCACCTCTTTTCTTGGTGGCGATATAGATAACGCAATTCAGGCTTGTGCTTCCTTAGAACAGCAAGAACTCCTGGAAGAACTCGCTGAATCTGTAGGAGCTCCGGCTGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003747 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006412 GO:0006415 GO:0006518 GO:0006807 GO:0006996 GO:0008079 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009668 GO:0009987 GO:0010027 GO:0010467 GO:0016043 GO:0019538 GO:0022411 GO:0032544 GO:0032984 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0043624 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0061024 GO:0071704 GO:0071840 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PCRF RF-1

Protein Analysis

417

Amino Acids

46.44

Weight (kDa)

6.26

Isoelectric Point (pI)

38.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PCRF	PF03462	62 - 256	1.4e-70	PCRF domain
RF-1	PF00472	263 - 373	5.9e-38	RF-1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014575)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G62910
fragaria_vesca	FvH4_2g25000
malus_domestica	MD03G1280500.v1.1 MD11G1301500.v1.1
prunus_persica	Prupe.8G255000_v2.0.a1
pyrus_communis	pycom03g22220 pycom11g26410
rosa_chinensis	RchiOBHm_Chr6g0292981
rosa_laevigata	RLG00000011977
rosa_multiflora	Rmu_sc0001788.1_g000007
rosa_roxburghii	Rroxscaffold_7G00174500
rosa_rugosa	Rorug06G0231600
rosa_samantha	Rh6AG344400 Rh6BG351700 Rh6CG358900 Rh6DG344500
rosa_wichuraiana	Rw6G030060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	533
Acc16I	TGCGCA	1 cut(s)	264
Acc36I	ACCTGC	2 cut(s)	533, 1036
AccI	GTMKAC	1 cut(s)	672
AccII	CGCG	3 cut(s)	69, 94, 744
AciI	CCGC	6 cut(s)	69, 149, 785, 916, 979, 1036
AclWI	GGATC	1 cut(s)	215
AcuI	CTGAAG	1 cut(s)	932
AfaI	GTAC	3 cut(s)	514, 572, 1054
AfiI	CCNNNNNNNGG	1 cut(s)	1116
AflIII	ACRYGT	2 cut(s)	667, 715
AgsI	TTSAA	7 cut(s)	313, 401, 407, 437, 500, 713, 824
AhdI	GACNNNNNGTC	2 cut(s)	85, 232
AjnI	CCWGG	2 cut(s)	191, 1209
Alw21I	GWGCWC	4 cut(s)	32, 130, 1140, 1240
Alw26I	GTCTC	4 cut(s)	332, 610, 872, 945
AlwI	GGATC	1 cut(s)	215
AlwNI	CAGNNNCTG	2 cut(s)	620, 976
AoxI	GGCC	2 cut(s)	16, 639
ApeKI	GCWGC	3 cut(s)	5, 976, 1012
Asp700I	GAANNNNTTC	1 cut(s)	1074
AspLEI	GCGC	2 cut(s)	96, 265
AspS9I	GGNCC	1 cut(s)	79
AsuC2I	CCSGG	1 cut(s)	77
AsuHPI	GGTGA	3 cut(s)	533, 557, 1132
AvaII	GGWCC	1 cut(s)	79
BanII	GRGCYC	5 cut(s)	32, 130, 156, 1140, 1240
BauI	CACGAG	1 cut(s)	1062
BbsI	GAAGAC	2 cut(s)	81, 194
Bbv12I	GWGCWC	4 cut(s)	32, 130, 1140, 1240
BbvI	GCAGC	2 cut(s)	963, 1024
BccI	CCATC	2 cut(s)	643, 1122
BciT130I	CCWGG	2 cut(s)	193, 1211
BciVI	GTATCC	1 cut(s)	626
BcnI	CCSGG	1 cut(s)	77
BcoDI	GTCTC	4 cut(s)	332, 610, 872, 945
BfaI	CTAG	1 cut(s)	465
BfmI	CTRYAG	3 cut(s)	180, 301, 1230
BfuAI	ACCTGC	2 cut(s)	533, 1036
BfuI	GTATCC	1 cut(s)	626
BisI	GCNGC	3 cut(s)	6, 977, 1013
BlsI	GCNGC	3 cut(s)	7, 978, 1014
Bme1390I	CCNGG	3 cut(s)	77, 193, 1211
Bme18I	GGWCC	1 cut(s)	79
BmeRI	GACNNNNNGTC	2 cut(s)	85, 232
BmgT120I	GGNCC	1 cut(s)	79
BmiI	GGNNCC	2 cut(s)	153, 850
BmrFI	CCNGG	3 cut(s)	77, 193, 1211
BmrI	ACTGGG	1 cut(s)	237
BmsI	GCATC	5 cut(s)	441, 469, 921, 927, 967
BmuI	ACTGGG	1 cut(s)	237
BpiI	GAAGAC	2 cut(s)	81, 194
BplI	GAGNNNNNCTC	4 cut(s)	19, 51, 1127, 1159
BpuEI	CTTGAG	2 cut(s)	431, 592
BpuMI	CCSGG	1 cut(s)	77
BsaI	GGTCTC	1 cut(s)	945
BsaJI	CCNNGG	1 cut(s)	99
Bsc4I	CCNNNNNNNGG	1 cut(s)	1116
Bse1I	ACTGG	3 cut(s)	243, 520, 1060
Bse3DI	GCAATG	2 cut(s)	15, 373
BseBI	CCWGG	2 cut(s)	193, 1211
BseDI	CCNNGG	1 cut(s)	99
BseGI	GGATG	2 cut(s)	793, 912
BseLI	CCNNNNNNNGG	1 cut(s)	1116
BseMI	GCAATG	2 cut(s)	15, 373
BseMII	CTCAG	1 cut(s)	627
BseNI	ACTGG	3 cut(s)	243, 520, 1060
BseRI	GAGGAG	2 cut(s)	108, 642
BseXI	GCAGC	2 cut(s)	963, 1024
BsgI	GTGCAG	1 cut(s)	1086
Bsh1236I	CGCG	3 cut(s)	69, 94, 744
BshFI	GGCC	2 cut(s)	18, 641
BsiHKAI	GWGCWC	4 cut(s)	32, 130, 1140, 1240
BsiSI	CCGG	3 cut(s)	77, 852, 1241
BslI	CCNNNNNNNGG	1 cut(s)	1116
BsmAI	GTCTC	4 cut(s)	332, 610, 872, 945
BsnI	GGCC	2 cut(s)	18, 641
Bso31I	GGTCTC	1 cut(s)	945
Bsp1286I	GDGCHC	5 cut(s)	32, 130, 156, 1140, 1240
Bsp143I	GATC	4 cut(s)	220, 547, 771, 889
BspACI	CCGC	6 cut(s)	69, 149, 785, 916, 979, 1036
BspANI	GGCC	2 cut(s)	18, 641
BspCNI	CTCAG	1 cut(s)	626
BspFNI	CGCG	3 cut(s)	69, 94, 744
BspLI	GGNNCC	2 cut(s)	153, 850
BspMAI	CTGCAG	1 cut(s)	184
BspMI	ACCTGC	2 cut(s)	533, 1036
BspPI	GGATC	1 cut(s)	215
BspTNI	GGTCTC	1 cut(s)	945
BsrDI	GCAATG	2 cut(s)	15, 373
BsrI	ACTGG	3 cut(s)	243, 520, 1060
BssECI	CCNNGG	1 cut(s)	99
BssMI	GATC	4 cut(s)	220, 547, 771, 889
BssNAI	GTATAC	1 cut(s)	673
BssSI	CACGAG	1 cut(s)	1062
Bst1107I	GTATAC	1 cut(s)	673
Bst2BI	CACGAG	1 cut(s)	1062
Bst2UI	CCWGG	2 cut(s)	193, 1211
Bst4CI	ACNGT	3 cut(s)	575, 604, 766
Bst6I	CTCTTC	1 cut(s)	330
BstC8I	GCNNGC	6 cut(s)	216, 261, 364, 746, 981, 1246
BstDEI	CTNAG	2 cut(s)	613, 1192
BstDSI	CCRYGG	1 cut(s)	99
BstF5I	GGATG	2 cut(s)	793, 912
BstFNI	CGCG	3 cut(s)	69, 94, 744
BstHHI	GCGC	2 cut(s)	96, 265
BstKTI	GATC	4 cut(s)	223, 550, 774, 892
BstMAI	GTCTC	4 cut(s)	332, 610, 872, 945
BstMBI	GATC	4 cut(s)	220, 547, 771, 889
BstMWI	GCNNNNNNNGC	5 cut(s)	359, 451, 767, 1042, 1178
BstNI	CCWGG	2 cut(s)	193, 1211
BstSCI	CCNGG	3 cut(s)	75, 191, 1209
BstSFI	CTRYAG	3 cut(s)	180, 301, 1230
BstUI	CGCG	3 cut(s)	69, 94, 744
BstV1I	GCAGC	2 cut(s)	963, 1024
BstV2I	GAAGAC	2 cut(s)	81, 194
BstZ17I	GTATAC	1 cut(s)	673
BsuI	GTATCC	1 cut(s)	626
BsuRI	GGCC	2 cut(s)	18, 641
BtgI	CCRYGG	1 cut(s)	99
BtsCI	GGATG	2 cut(s)	793, 912
BtsI	GCAGTG	1 cut(s)	756
BtsIMutI	CAGTG	3 cut(s)	250, 580, 756
BveI	ACCTGC	2 cut(s)	533, 1036
Cac8I	GCNNGC	6 cut(s)	216, 261, 364, 746, 981, 1246
CaiI	CAGNNNCTG	2 cut(s)	620, 976
CfoI	GCGC	2 cut(s)	96, 265
Cfr13I	GGNCC	1 cut(s)	79
CseI	GACGC	1 cut(s)	75
Csp6I	GTAC	3 cut(s)	513, 571, 1053
CviAII	CATG	2 cut(s)	145, 775
CviQI	GTAC	3 cut(s)	513, 571, 1053
DdeI	CTNAG	2 cut(s)	613, 1192
DpnI	GATC	4 cut(s)	222, 549, 773, 891
DpnII	GATC	4 cut(s)	220, 547, 771, 889
DriI	GACNNNNNGTC	2 cut(s)	85, 232
Eam1104I	CTCTTC	1 cut(s)	330
Eam1105I	GACNNNNNGTC	2 cut(s)	85, 232
EarI	CTCTTC	1 cut(s)	330
EciI	GGCGGA	2 cut(s)	164, 800
Ecl136II	GAGCTC	4 cut(s)	30, 128, 1138, 1238
Eco147I	AGGCCT	1 cut(s)	641
Eco24I	GRGCYC	5 cut(s)	32, 130, 156, 1140, 1240
Eco31I	GGTCTC	1 cut(s)	945
Eco32I	GATATC	1 cut(s)	393
Eco47I	GGWCC	1 cut(s)	79
Eco53kI	GAGCTC	4 cut(s)	30, 128, 1138, 1238
Eco57I	CTGAAG	1 cut(s)	932
EcoICRI	GAGCTC	4 cut(s)	30, 128, 1138, 1238
EcoRII	CCWGG	2 cut(s)	191, 1209
EcoRV	GATATC	1 cut(s)	393
EcoT38I	GRGCYC	5 cut(s)	32, 130, 156, 1140, 1240
FaeI	CATG	2 cut(s)	148, 778
FatI	CATG	2 cut(s)	144, 774
FauI	CCCGC	1 cut(s)	972
FblI	GTMKAC	1 cut(s)	672
Fnu4HI	GCNGC	3 cut(s)	6, 977, 1013
FokI	GGATG	2 cut(s)	800, 899
FriOI	GRGCYC	5 cut(s)	32, 130, 156, 1140, 1240
Fsp4HI	GCNGC	3 cut(s)	6, 977, 1013
FspBI	CTAG	1 cut(s)	465
FspI	TGCGCA	1 cut(s)	264
GlaI	GCGC	2 cut(s)	95, 264
GluI	GCNGC	3 cut(s)	6, 977, 1013
HaeIII	GGCC	2 cut(s)	18, 641
HapII	CCGG	3 cut(s)	77, 852, 1241
HgaI	GACGC	1 cut(s)	75
HhaI	GCGC	2 cut(s)	96, 265
Hin1II	CATG	2 cut(s)	148, 778
Hin6I	GCGC	2 cut(s)	94, 263
HinP1I	GCGC	2 cut(s)	94, 263
HincII	GTYRAC	1 cut(s)	868
HindII	GTYRAC	1 cut(s)	868
HindIII	AAGCTT	2 cut(s)	257, 345
HinfI	GANTC	9 cut(s)	135, 176, 317, 407, 534, 692, 1030, 1075, 1226
HpaII	CCGG	3 cut(s)	77, 852, 1241
HphI	GGTGA	3 cut(s)	533, 557, 1132
Hpy166II	GTNNAC	3 cut(s)	113, 673, 868
Hpy188I	TCNGA	4 cut(s)	322, 616, 951, 1080
Hpy188III	TCNNGA	5 cut(s)	42, 224, 292, 448, 476
Hpy8I	GTNNAC	3 cut(s)	113, 673, 868
HpyAV	CCTTC	2 cut(s)	48, 470
HpyCH4III	ACNGT	3 cut(s)	575, 604, 766
HpyCH4IV	ACGT	3 cut(s)	228, 667, 715
HpyCH4V	TGCA	6 cut(s)	8, 144, 182, 482, 748, 1067
HpyF10VI	GCNNNNNNNGC	5 cut(s)	359, 451, 767, 1042, 1178
HpyF3I	CTNAG	2 cut(s)	613, 1192
HpySE526I	ACGT	3 cut(s)	228, 667, 715
Hsp92II	CATG	2 cut(s)	148, 778
HspAI	GCGC	2 cut(s)	94, 263
Kzo9I	GATC	4 cut(s)	220, 547, 771, 889
LmnI	GCTCC	7 cut(s)	35, 101, 133, 151, 848, 1235, 1243
Lsp1109I	GCAGC	2 cut(s)	963, 1024
LweI	GCATC	5 cut(s)	441, 469, 921, 927, 967
MaeI	CTAG	1 cut(s)	465
MaeII	ACGT	3 cut(s)	228, 667, 715
MaeIII	GTNAC	3 cut(s)	292, 467, 1105
MalI	GATC	4 cut(s)	222, 549, 773, 891
MboI	GATC	4 cut(s)	220, 547, 771, 889
MboII	GAAGA	5 cut(s)	81, 199, 347, 944, 1226
MhlI	GDGCHC	5 cut(s)	32, 130, 156, 1140, 1240
MluCI	AATT	4 cut(s)	586, 680, 986, 1173
MlyI	GAGTC	2 cut(s)	129, 311
MroXI	GAANNNNTTC	1 cut(s)	1074
MseI	TTAA	3 cut(s)	683, 1092, 1121
MspA1I	CMGCKG	2 cut(s)	884, 1036
MspI	CCGG	3 cut(s)	77, 852, 1241
MspR9I	CCNGG	3 cut(s)	77, 193, 1211
MvaI	CCWGG	2 cut(s)	193, 1211
MvnI	CGCG	3 cut(s)	69, 94, 744
MwoI	GCNNNNNNNGC	5 cut(s)	359, 451, 767, 1042, 1178
NciI	CCSGG	1 cut(s)	77
NdeII	GATC	4 cut(s)	220, 547, 771, 889
NlaIII	CATG	2 cut(s)	148, 778
NlaIV	GGNNCC	2 cut(s)	153, 850
NmuCI	GTSAC	3 cut(s)	292, 467, 1105
NsbI	TGCGCA	1 cut(s)	264
PaqCI	CACCTGC	1 cut(s)	533
PceI	AGGCCT	1 cut(s)	641
PcsI	WCGNNNNNNNCGW	1 cut(s)	712
PdmI	GAANNNNTTC	1 cut(s)	1074
PfeI	GAWTC	7 cut(s)	176, 407, 534, 692, 1030, 1075, 1226
PfoI	TCCNGGA	2 cut(s)	75, 1209
PkrI	GCNGC	3 cut(s)	7, 978, 1014
PleI	GAGTC	2 cut(s)	129, 311
PpsI	GAGTC	2 cut(s)	129, 311
Psp124BI	GAGCTC	4 cut(s)	32, 130, 1140, 1240
Psp6I	CCWGG	2 cut(s)	191, 1209
PspGI	CCWGG	2 cut(s)	191, 1209
PspN4I	GGNNCC	2 cut(s)	153, 850
PspPI	GGNCC	1 cut(s)	79
PstI	CTGCAG	1 cut(s)	184
PstNI	CAGNNNCTG	2 cut(s)	620, 976
PvuII	CAGCTG	1 cut(s)	884
RsaI	GTAC	3 cut(s)	514, 572, 1054
RsaNI	GTAC	3 cut(s)	513, 571, 1053
SacI	GAGCTC	4 cut(s)	32, 130, 1140, 1240
SaqAI	TTAA	3 cut(s)	683, 1092, 1121
SatI	GCNGC	3 cut(s)	6, 977, 1013
Sau3AI	GATC	4 cut(s)	220, 547, 771, 889
Sau96I	GGNCC	1 cut(s)	79
SchI	GAGTC	2 cut(s)	129, 311
ScrFI	CCNGG	3 cut(s)	77, 193, 1211
SduI	GDGCHC	5 cut(s)	32, 130, 156, 1140, 1240
SfaNI	GCATC	5 cut(s)	441, 469, 921, 927, 967
SfcI	CTRYAG	3 cut(s)	180, 301, 1230
SinI	GGWCC	1 cut(s)	79
SmlI	CTYRAG	2 cut(s)	446, 607
SmoI	CTYRAG	2 cut(s)	446, 607
Sse9I	AATT	4 cut(s)	586, 680, 986, 1173
SseBI	AGGCCT	1 cut(s)	641
SsiI	CCGC	6 cut(s)	69, 149, 785, 916, 979, 1036
SspMI	CTAG	1 cut(s)	465
SstI	GAGCTC	4 cut(s)	32, 130, 1140, 1240
StuI	AGGCCT	1 cut(s)	641
StyD4I	CCNGG	3 cut(s)	75, 191, 1209
TaaI	ACNGT	3 cut(s)	575, 604, 766
TaiI	ACGT	3 cut(s)	231, 670, 718
TaqI	TCGA	2 cut(s)	43, 807
TaqII	GACCGA	2 cut(s)	196, 1124
TasI	AATT	4 cut(s)	586, 680, 986, 1173
TatI	WGTACW	1 cut(s)	570
TfiI	GAWTC	7 cut(s)	176, 407, 534, 692, 1030, 1075, 1226
Tru1I	TTAA	3 cut(s)	683, 1092, 1121
Tru9I	TTAA	3 cut(s)	683, 1092, 1121
TscAI	CASTG	3 cut(s)	250, 580, 763
TseFI	GTSAC	3 cut(s)	292, 467, 1105
TseI	GCWGC	3 cut(s)	5, 976, 1012
Tsp45I	GTSAC	3 cut(s)	292, 467, 1105
TspDTI	ATGAA	4 cut(s)	540, 692, 705, 804
TspGWI	ACGGA	2 cut(s)	88, 545
TspRI	CASTG	3 cut(s)	250, 580, 763
VpaK11BI	GGWCC	1 cut(s)	79
XmiI	GTMKAC	1 cut(s)	672
XmnI	GAANNNNTTC	1 cut(s)	1074
XspI	CTAG	1 cut(s)	465

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.