MD03G1294100.v1.1

Histone-lysine N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005087
Physical Location & Seq
Forward (+)
37044296 .. 37049505
5210 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1294100.v1.1.491

Sequence Viewer

Length: 2451 bp
ATGCTGTCCCAAGCTTCCGACTCTGCAACTGAACTCACAAAATTCCATGGAGAAGATCCGAGTGATGGCATCGGAAGTTTGGAGCAGAAGATGCACCAGCTCAAGTTGCAAATTCAAGCAGAGAGGGTGATATTAGTAAAAGAAAAAGTTAAGAAGAACAGGGAGAAGGTAGAAGGTTATGTTTCAAAAACTATATCGGAGACATCAAGAGCGAATTTGAATGCTTCGTCATCAGAGCAAAATGGGAGCTTTAAATTGTTTTCTTCAAGAATTGAACAGCCTCTCTGCAAAATCAGTGGATTTGCGCAGGGATATGGTGAGAAAAACTGGATTGAGAATCAAGAGGTTTTATTCTCATCCAGTACCAAGCTCCCACTTGTTGACAAGATACCGCCTTATACAACTTGGATATTCTTGGACAGAAATCAAAGAATGGCTGAAGACCAATCAGTAGTTGGGAGGAGGCGTATTTACTACGATGAAGATGGCAGTGAAGCTCTTGTCTACAGTGGCAGTGATGATGAGGCAGAAGAACCCGAAAAAGTAAAACATGAATTTTCTGCGGGCGAAGATCGAATTCTGTTGATGGCCTTTCAGGAGCATGGCCTAGGCGAGGAAGTAGTGGAAGTTGTGAGGGAGTTTATTGGAGTCCCCATTTTGGAAATCCTGGACCGTTATAACACAATCAAGGAAAGGAACCGCGAGAAACGTGAACCCGATGGGTGCATTTCTCTTGATAAGAGTCTTAGTGCTGCATTAGATTCTTTCGATAACCTTTTCTGTCGCCGTTGCTTGGTGTTTGATTGTCGTTTGCATGGATGTTCTCAACCTTTAATATATCCTAGTGAAAAGTCGCAGTGGTCCGGTCATGACGAGAACCAGGAACCTTGCAGTGATCAATGTTACCTCAGGTTGAGTATAGTAACATCTGAGAGAGAAAGTGCTCCAGGATCATCCATTAGTGCCGAGCGACCAAGTTCTCTTGGCTATACAGACCTCACACACAACGAAAGAAGCATTCCTGGAGATGCTGAGACTACAACTTCAGAAACCATTCAGTGTTCTCATATCCTGAAGATGCACAATGAAAATACAGGGAAGCGCAAGGTCATGAAGCATACAGATAAAGTGGCAAAGGACCTAACTATAGTACCTGATGATTTCCACGGTTCTTCTAAGAAACAAAAGAGATTAGATGCTTTGGATTTGGTAACTGCAACTAGTGAACCTATCCCTGTTCGGGTTCACATTTCCAGAGGTGAACCAAGAGATGTAGCCGAAGTGCCTGAACTGAGGCAAACATCCAATTCTACACGTGGACAAGTTGAGGGGATGTGTAGCAACTCTGAGTGGAAACCAGTGGAGAAAGATTTATACATGAAGGGACTACAGATATTTGGGAGAAACAGTTGCCTCATAGCGAGGAACTTACTCTCCGGATTGAGGACGTGTAAGGAAGTGTCTAGTTACATGCATAATTCTGGATCTTCAACGCCTAACAGATCTGTGGTTGAACCATCGTCATTTATGGAAGACAATGTGAAAGCTGATTTGGATCAGACAGAACAAGAGATGTCGTCAAAGCCACGGTTACTTCGTAGAAGGGGAAAAGCACGGAGGCTGAAGTATTCTTGGAAGTCTGCTGGGCACCCAACAATGTGGAAAAGGATTGCTGATGGCAAAAACGATTCACTTAAACTGTATACGCCATGTGAATGCCAGTCTATGTGTGGAAAGGAATGTTCTTGTATGAGTAATGGAACCTGCTGTGAAAAATACTGTGGGTGCTCAAAGAGTTGCAAAAATAGGTTCAGGGGATGTCACTGTGCAAAGAGTCAGTGTAGAAGTCGGCAGTGCCCATGCTTTGCTGCTGGGCGCGAATGTGACCCGGATGTCTGTCGTCATTGTTGGATTAGTTGTGGGGATGGTTCACTAGGCGAGCCACCGAAACAGGGAGATAGTCAGTGTGGTAACATGAGGCTTCTTCTAAGACAACAACAGAGGATTCTCCTGGGCAAGTCTGATGTTGCTGGTTGGGGAGCCTTCTTAAAGAACCCCGTAAACAAGAATGATTATCTTGGAGAATATACTGGTGAAATCATCTCGCATCAGGAAGCAGACAAGCGGGGGAAGATTTATGATCGTGTAAACTCATCATTCCTTTTCGACTTGAATGAACAGTATGTCCTAGATGCTTACCGAAAGGGAGACAAGCTTAAATTTGCAAACCACTCATCAAATCCTAACTGCCATGCAAAGGTAATGCTGGTTGCGGGTGATCATCGAGTTGGCATATTTGCCAAGGAGCATATTGATGCCGGTGAGGAGCTCTTCTACGATTATTGTTATCTATCAGAGGCAGCACCTGTATGGGCTCAAAAACCGGAGGGTTCTAAGAGGGACGATTCATCTGTCTCCCGAGGCAGAGCGAAAAAGCACCAAGCCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0003723 GO:0003727 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005677 GO:0005911 GO:0006139 GO:0006325 GO:0006342 GO:0006349 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008213 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009642 GO:0009646 GO:0009653 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0017053 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0030054 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031519 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048316 GO:0048317 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048587 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0055044 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070734 GO:0071514 GO:0071704 GO:0071840 GO:0080050 GO:0080090 GO:0090568 GO:0090698 GO:0097159 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:2000014 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00310 ko05206 map00310 map05206
Pfam Domains
Protein Families
SET

Protein Analysis

817

Amino Acids

91.88

Weight (kDa)

7.23

Isoelectric Point (pI)

50.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_CLF_N PF25996 186 - 230 1.6e-16 Histone-lysine N-methyltransferase CLF, N-terminal HTH domain
preSET_CXC PF18264 606 - 637 2.7e-07 CXC domain
SET PF00856 678 - 781 2.2e-20 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 678
AasI GACNNNNNNGTC 1 cut(s) 1892
Acc16I TGCGCA 1 cut(s) 306
Acc36I ACCTGC 1 cut(s) 1772
AccB1I GGYRCC 1 cut(s) 1647
AccI GTMKAC 2 cut(s) 504, 1703
AccII CGCG 2 cut(s) 702, 1878
AccIII TCCGGA 1 cut(s) 1436
AciI CCGC 5 cut(s) 392, 563, 700, 2125, 2273
AclWI GGATC 4 cut(s) 50, 958, 1492, 1563
AcsI RAATTY 6 cut(s) 41, 111, 214, 554, 576, 2219
AcuI CTGAAG 4 cut(s) 459, 1029, 1094, 1643
AcvI CACGTG 1 cut(s) 1316
AfaI GTAC 2 cut(s) 364, 1152
AfiI CCNNNNNNNGG 8 cut(s) 65, 613, 658, 793, 1240, 1443, 1952, 2257
AflIII ACRYGT 2 cut(s) 1313, 1448
AgsI TTSAA 8 cut(s) 116, 186, 220, 267, 275, 1491, 1514, 2173
AhlI ACTAGT 1 cut(s) 1220
AjiI CACGTC 1 cut(s) 1449
AjnI CCWGG 5 cut(s) 666, 879, 946, 1021, 2010
AjuI GAANNNNNNNTTGG 2 cut(s) 1535, 1567
AloI GAACNNNNNNTCC 4 cut(s) 1418, 1450, 2169, 2201
AluBI AGCT 8 cut(s) 14, 100, 249, 370, 497, 1547, 2215, 2329
AluI AGCT 8 cut(s) 14, 100, 249, 370, 497, 1547, 2215, 2329
Alw21I GWGCWC 3 cut(s) 946, 1790, 2331
Alw26I GTCTC 4 cut(s) 194, 1028, 2202, 2419
AlwI GGATC 4 cut(s) 50, 958, 1492, 1563
AlwNI CAGNNNCTG 1 cut(s) 2366
Ama87I CYCGRG 1 cut(s) 2418
Aor13HI TCCGGA 1 cut(s) 1436
AoxI GGCC 2 cut(s) 588, 604
ApeKI GCWGC 3 cut(s) 752, 1868, 2360
ApoI RAATTY 6 cut(s) 41, 111, 214, 554, 576, 2219
Asp700I GAANNNNTTC 1 cut(s) 1053
AspA2I CCTAGG 1 cut(s) 607
AspLEI GCGC 3 cut(s) 307, 1104, 1878
AspS9I GGNCC 3 cut(s) 670, 861, 1138
AsuC2I CCSGG 1 cut(s) 1889
AsuHPI GGTGA 6 cut(s) 139, 329, 1271, 2105, 2288, 2333
AvaI CYCGRG 1 cut(s) 2418
AvaII GGWCC 3 cut(s) 670, 861, 1138
AvrII CCTAGG 1 cut(s) 607
AxyI CCTNAGG 1 cut(s) 908
BaeGI GKGCMC 2 cut(s) 1650, 1859
BaeI ACNNNNGTAYC 2 cut(s) 1134, 1167
BanI GGYRCC 1 cut(s) 1647
BanII GRGCYC 2 cut(s) 2331, 2377
BbrPI CACGTG 1 cut(s) 1316
BbsI GAAGAC 2 cut(s) 447, 1539
Bbv12I GWGCWC 3 cut(s) 946, 1790, 2331
BbvI GCAGC 3 cut(s) 739, 1855, 2372
BccI CCATC 7 cut(s) 59, 479, 580, 713, 1525, 1670, 1919
BceAI ACGGC 1 cut(s) 771
BciT130I CCWGG 5 cut(s) 668, 881, 948, 1023, 2012
BclI TGATCA 2 cut(s) 895, 2278
BcnI CCSGG 1 cut(s) 1889
BcoDI GTCTC 4 cut(s) 194, 1028, 2202, 2419
BcuI ACTAGT 1 cut(s) 1220
BfaI CTAG 6 cut(s) 608, 843, 1221, 1464, 1934, 2189
BfmI CTRYAG 3 cut(s) 505, 1146, 1388
BfuAI ACCTGC 1 cut(s) 1772
BglII AGATCT 1 cut(s) 1502
BisI GCNGC 3 cut(s) 753, 1869, 2361
BlnI CCTAGG 1 cut(s) 607
BlsI GCNGC 3 cut(s) 754, 1870, 2362
Bme1390I CCNGG 6 cut(s) 668, 881, 948, 1023, 1889, 2012
Bme18I GGWCC 3 cut(s) 670, 861, 1138
BmeT110I CYCGRG 1 cut(s) 2418
BmgBI CACGTC 1 cut(s) 1449
BmgT120I GGNCC 3 cut(s) 670, 861, 1138
BmiI GGNNCC 5 cut(s) 698, 885, 1649, 1762, 2041
BmrFI CCNGG 6 cut(s) 668, 881, 948, 1023, 1889, 2012
BmsI GCATC 8 cut(s) 78, 81, 1018, 1068, 1186, 2116, 2182, 2305
BpiI GAAGAC 2 cut(s) 447, 1539
BpmI CTGGAG 2 cut(s) 930, 1044
BpuEI CTTGAG 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 1889
BsaAI YACGTR 1 cut(s) 1316
BsaBI GATNNNNATC 1 cut(s) 1554
BsaJI CCNNGG 7 cut(s) 46, 607, 1165, 1586, 2011, 2301, 2419
BsaWI WCCGGW 3 cut(s) 863, 1436, 2383
BsaXI ACNNNNNCTCC 6 cut(s) 451, 481, 1393, 1418, 1423, 1448
Bsc4I CCNNNNNNNGG 8 cut(s) 65, 613, 658, 793, 1240, 1443, 1952, 2257
Bse118I RCCGGY 1 cut(s) 2318
Bse1I ACTGG 5 cut(s) 332, 360, 1358, 1720, 2095
Bse21I CCTNAGG 1 cut(s) 908
Bse8I GATNNNNATC 1 cut(s) 1554
BseAI TCCGGA 1 cut(s) 1436
BseBI CCWGG 5 cut(s) 668, 881, 948, 1023, 2012
BseDI CCNNGG 7 cut(s) 46, 607, 1165, 1586, 2011, 2301, 2419
BseGI GGATG 8 cut(s) 356, 824, 953, 1301, 1338, 1823, 1897, 1930
BseJI GATNNNNATC 1 cut(s) 1554
BseLI CCNNNNNNNGG 8 cut(s) 65, 613, 658, 793, 1240, 1443, 1952, 2257
BseMII CTCAG 5 cut(s) 921, 922, 1023, 1283, 1338
BseNI ACTGG 5 cut(s) 332, 360, 1358, 1720, 2095
BseRI GAGGAG 2 cut(s) 475, 2339
BseSI GKGCMC 2 cut(s) 1650, 1859
BseXI GCAGC 3 cut(s) 739, 1855, 2372
BseYI CCCAGC 2 cut(s) 1643, 1871
Bsh1236I CGCG 2 cut(s) 702, 1878
BshFI GGCC 2 cut(s) 590, 606
BshNI GGYRCC 1 cut(s) 1647
BsiHKAI GWGCWC 3 cut(s) 946, 1790, 2331
BsiHKCI CYCGRG 1 cut(s) 2418
BsiSI CCGG 5 cut(s) 864, 1437, 1889, 2319, 2384
BslFI GGGAC 3 cut(s) 635, 1398, 2414
BslI CCNNNNNNNGG 8 cut(s) 65, 613, 658, 793, 1240, 1443, 1952, 2257
BsmAI GTCTC 4 cut(s) 194, 1028, 2202, 2419
BsmFI GGGAC 3 cut(s) 635, 1398, 2414
BsmI GAATGC 3 cut(s) 226, 1017, 1721
BsnI GGCC 2 cut(s) 590, 606
BsoBI CYCGRG 1 cut(s) 2418
Bsp1286I GDGCHC 6 cut(s) 946, 1650, 1790, 1859, 2331, 2377
Bsp13I TCCGGA 1 cut(s) 1436
Bsp143I GATC 9 cut(s) 55, 571, 895, 950, 1484, 1502, 1555, 2140, 2278
Bsp19I CCATGG 1 cut(s) 46
BspACI CCGC 5 cut(s) 392, 563, 700, 2125, 2273
BspANI GGCC 2 cut(s) 590, 606
BspCNI CTCAG 5 cut(s) 921, 922, 1024, 1284, 1339
BspEI TCCGGA 1 cut(s) 1436
BspFNI CGCG 2 cut(s) 702, 1878
BspHI TCATGA 2 cut(s) 868, 1110
BspLI GGNNCC 5 cut(s) 698, 885, 1649, 1762, 2041
BspMI ACCTGC 1 cut(s) 1772
BspPI GGATC 4 cut(s) 50, 958, 1492, 1563
BspQI GCTCTTC 1 cut(s) 2336
BspT107I GGYRCC 1 cut(s) 1647
BsrFI RCCGGY 1 cut(s) 2318
BsrI ACTGG 5 cut(s) 332, 360, 1358, 1720, 2095
BssAI RCCGGY 1 cut(s) 2318
BssECI CCNNGG 7 cut(s) 46, 607, 1165, 1586, 2011, 2301, 2419
BssMI GATC 9 cut(s) 55, 571, 895, 950, 1484, 1502, 1555, 2140, 2278
BssNAI GTATAC 1 cut(s) 1704
BssT1I CCWWGG 3 cut(s) 46, 607, 2301
Bst1107I GTATAC 1 cut(s) 1704
Bst2UI CCWGG 5 cut(s) 668, 881, 948, 1023, 2012
Bst4CI ACNGT 9 cut(s) 509, 674, 1169, 1409, 1590, 1701, 1781, 1826, 2181
Bst6I CTCTTC 1 cut(s) 2336
BstAPI GCANNNNNTGC 1 cut(s) 91
BstBAI YACGTR 1 cut(s) 1316
BstC8I GCNNGC 2 cut(s) 565, 1940
BstDEI CTNAG 9 cut(s) 746, 908, 930, 1032, 1176, 1292, 1347, 1988, 2394
BstDSI CCRYGG 3 cut(s) 46, 1165, 1586
BstENI CCTNNNNNAGG 1 cut(s) 611
BstF5I GGATG 8 cut(s) 356, 824, 953, 1301, 1338, 1823, 1897, 1930
BstFNI CGCG 2 cut(s) 702, 1878
BstHHI GCGC 3 cut(s) 307, 1104, 1878
BstKTI GATC 9 cut(s) 58, 574, 898, 953, 1487, 1505, 1558, 2143, 2281
BstMAI GTCTC 4 cut(s) 194, 1028, 2202, 2419
BstMBI GATC 9 cut(s) 55, 571, 895, 950, 1484, 1502, 1555, 2140, 2278
BstMWI GCNNNNNNNGC 2 cut(s) 91, 106
BstNI CCWGG 5 cut(s) 668, 881, 948, 1023, 2012
BstNSI RCATGY 1 cut(s) 1474
BstSCI CCNGG 6 cut(s) 666, 879, 946, 1021, 1887, 2010
BstSFI CTRYAG 3 cut(s) 505, 1146, 1388
BstSLI GKGCMC 2 cut(s) 1650, 1859
BstUI CGCG 2 cut(s) 702, 1878
BstV1I GCAGC 3 cut(s) 739, 1855, 2372
BstV2I GAAGAC 2 cut(s) 447, 1539
BstX2I RGATCY 3 cut(s) 55, 1484, 1502
BstXI CCANNNNNNTGG 1 cut(s) 1659
BstYI RGATCY 3 cut(s) 55, 1484, 1502
BstZ17I GTATAC 1 cut(s) 1704
Bsu36I CCTNAGG 1 cut(s) 908
BsuRI GGCC 2 cut(s) 590, 606
BtgI CCRYGG 3 cut(s) 46, 1165, 1586
BtrI CACGTC 1 cut(s) 1449
BtsCI GGATG 8 cut(s) 356, 824, 953, 1301, 1338, 1823, 1897, 1930
BtsI GCAGTG 5 cut(s) 496, 520, 863, 898, 1859
BveI ACCTGC 1 cut(s) 1772
Cac8I GCNNGC 2 cut(s) 565, 1940
CaiI CAGNNNCTG 1 cut(s) 2366
CciI TCATGA 2 cut(s) 868, 1110
CfoI GCGC 3 cut(s) 307, 1104, 1878
Cfr10I RCCGGY 1 cut(s) 2318
Cfr13I GGNCC 3 cut(s) 670, 861, 1138
Csp6I GTAC 2 cut(s) 363, 1151
CspCI CAANNNNNGTGG 2 cut(s) 277, 312
CviQI GTAC 2 cut(s) 363, 1151
DdeI CTNAG 9 cut(s) 746, 908, 930, 1032, 1176, 1292, 1347, 1988, 2394
DpnI GATC 9 cut(s) 57, 573, 897, 952, 1486, 1504, 1557, 2142, 2280
DpnII GATC 9 cut(s) 55, 571, 895, 950, 1484, 1502, 1555, 2140, 2278
DraI TTTAAA 1 cut(s) 253
DrdI GACNNNNNNGTC 1 cut(s) 1892
DseDI GACNNNNNNGTC 1 cut(s) 1892
Eam1104I CTCTTC 1 cut(s) 2336
EarI CTCTTC 1 cut(s) 2336
Ecl136II GAGCTC 1 cut(s) 2329
Eco130I CCWWGG 3 cut(s) 46, 607, 2301
Eco24I GRGCYC 2 cut(s) 2331, 2377
Eco47I GGWCC 3 cut(s) 670, 861, 1138
Eco53kI GAGCTC 1 cut(s) 2329
Eco57I CTGAAG 4 cut(s) 459, 1029, 1094, 1643
Eco72I CACGTG 1 cut(s) 1316
Eco81I CCTNAGG 1 cut(s) 908
Eco88I CYCGRG 1 cut(s) 2418
EcoICRI GAGCTC 1 cut(s) 2329
EcoNI CCTNNNNNAGG 1 cut(s) 611
EcoO109I RGGNCCY 1 cut(s) 1138
EcoRI GAATTC 1 cut(s) 576
EcoRII CCWGG 5 cut(s) 666, 879, 946, 1021, 2010
EcoT14I CCWWGG 3 cut(s) 46, 607, 2301
EcoT22I ATGCAT 1 cut(s) 1476
EcoT38I GRGCYC 2 cut(s) 2331, 2377
ErhI CCWWGG 3 cut(s) 46, 607, 2301
FaqI GGGAC 3 cut(s) 635, 1398, 2414
FauI CCCGC 3 cut(s) 556, 2118, 2266
FbaI TGATCA 2 cut(s) 895, 2278
FblI GTMKAC 2 cut(s) 504, 1703
Fnu4HI GCNGC 3 cut(s) 753, 1869, 2361
FokI GGATG 8 cut(s) 343, 831, 940, 1288, 1345, 1830, 1904, 1937
FriOI GRGCYC 2 cut(s) 2331, 2377
Fsp4HI GCNGC 3 cut(s) 753, 1869, 2361
FspBI CTAG 6 cut(s) 608, 843, 1221, 1464, 1934, 2189
FspI TGCGCA 1 cut(s) 306
GlaI GCGC 3 cut(s) 306, 1103, 1877
GluI GCNGC 3 cut(s) 753, 1869, 2361
GsaI CCCAGC 2 cut(s) 1647, 1875
GsuI CTGGAG 2 cut(s) 930, 1044
HaeIII GGCC 2 cut(s) 590, 606
HapII CCGG 5 cut(s) 864, 1437, 1889, 2319, 2384
HhaI GCGC 3 cut(s) 307, 1104, 1878
Hin6I GCGC 3 cut(s) 305, 1102, 1876
HinP1I GCGC 3 cut(s) 305, 1102, 1876
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HindIII AAGCTT 2 cut(s) 12, 2213
HinfI GANTC 9 cut(s) 20, 337, 648, 742, 761, 1688, 1834, 2005, 2405
HpaII CCGG 5 cut(s) 864, 1437, 1889, 2319, 2384
HphI GGTGA 6 cut(s) 139, 329, 1271, 2105, 2288, 2333
HpyAV CCTTC 5 cut(s) 160, 167, 1375, 1596, 2053
HpyCH4III ACNGT 9 cut(s) 509, 674, 1169, 1409, 1590, 1701, 1781, 1826, 2181
HpyCH4IV ACGT 3 cut(s) 709, 1315, 1448
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 106
HpyF3I CTNAG 9 cut(s) 746, 908, 930, 1032, 1176, 1292, 1347, 1988, 2394
HpySE526I ACGT 3 cut(s) 709, 1315, 1448
HspAI GCGC 3 cut(s) 305, 1102, 1876
Kpn2I TCCGGA 1 cut(s) 1436
Ksp22I TGATCA 2 cut(s) 895, 2278
Kzo9I GATC 9 cut(s) 55, 571, 895, 950, 1484, 1502, 1555, 2140, 2278
LguI GCTCTTC 1 cut(s) 2336
LmnI GCTCC 8 cut(s) 82, 246, 375, 598, 949, 2039, 2305, 2326
Lsp1109I GCAGC 3 cut(s) 739, 1855, 2372
LweI GCATC 8 cut(s) 78, 81, 1018, 1068, 1186, 2116, 2182, 2305
MaeI CTAG 6 cut(s) 608, 843, 1221, 1464, 1934, 2189
MaeII ACGT 3 cut(s) 709, 1315, 1448
MaeIII GTNAC 8 cut(s) 902, 922, 1210, 1466, 1590, 1820, 1883, 1970
MalI GATC 9 cut(s) 57, 573, 897, 952, 1486, 1504, 1557, 2142, 2280
MboI GATC 9 cut(s) 55, 571, 895, 950, 1484, 1502, 1555, 2140, 2278
MflI RGATCY 3 cut(s) 55, 1484, 1502
MhlI GDGCHC 6 cut(s) 946, 1650, 1790, 1859, 2331, 2377
MlyI GAGTC 4 cut(s) 14, 657, 751, 1843
MmeI TCCRAC 2 cut(s) 42, 1889
Mph1103I ATGCAT 1 cut(s) 1476
MroI TCCGGA 1 cut(s) 1436
MroXI GAANNNNTTC 1 cut(s) 1053
MseI TTAA 6 cut(s) 150, 252, 833, 1695, 2048, 2217
MslI CAYNNNNRTG 3 cut(s) 1714, 2313, 2368
MspI CCGG 5 cut(s) 864, 1437, 1889, 2319, 2384
MspR9I CCNGG 6 cut(s) 668, 881, 948, 1023, 1889, 2012
Mva1269I GAATGC 3 cut(s) 226, 1017, 1721
MvaI CCWGG 5 cut(s) 668, 881, 948, 1023, 2012
MvnI CGCG 2 cut(s) 702, 1878
MwoI GCNNNNNNNGC 2 cut(s) 91, 106
NciI CCSGG 1 cut(s) 1889
NcoI CCATGG 1 cut(s) 46
NdeII GATC 9 cut(s) 55, 571, 895, 950, 1484, 1502, 1555, 2140, 2278
NlaIV GGNNCC 5 cut(s) 698, 885, 1649, 1762, 2041
NmeAIII GCCGAG 1 cut(s) 991
NmuCI GTSAC 2 cut(s) 1820, 1883
NsbI TGCGCA 1 cut(s) 306
NsiI ATGCAT 1 cut(s) 1476
NspI RCATGY 1 cut(s) 1474
PagI TCATGA 2 cut(s) 868, 1110
PciSI GCTCTTC 1 cut(s) 2336
PcsI WCGNNNNNNNCGW 1 cut(s) 1594
PctI GAATGC 3 cut(s) 226, 1017, 1721
PdmI GAANNNNTTC 1 cut(s) 1053
PfeI GAWTC 5 cut(s) 337, 761, 1688, 2005, 2405
PfoI TCCNGGA 3 cut(s) 666, 946, 1021
PkrI GCNGC 3 cut(s) 754, 1870, 2362
PleI GAGTC 4 cut(s) 14, 656, 750, 1842
PmaCI CACGTG 1 cut(s) 1316
PmlI CACGTG 1 cut(s) 1316
PpsI GAGTC 4 cut(s) 14, 656, 750, 1842
Ppu21I YACGTR 1 cut(s) 1316
PpuMI RGGWCCY 1 cut(s) 1138
PsiI TTATAA 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 2331
Psp5II RGGWCCY 1 cut(s) 1138
Psp6I CCWGG 5 cut(s) 666, 879, 946, 1021, 2010
PspCI CACGTG 1 cut(s) 1316
PspFI CCCAGC 2 cut(s) 1643, 1871
PspGI CCWGG 5 cut(s) 666, 879, 946, 1021, 2010
PspN4I GGNNCC 5 cut(s) 698, 885, 1649, 1762, 2041
PspPI GGNCC 3 cut(s) 670, 861, 1138
PspPPI RGGWCCY 1 cut(s) 1138
PstNI CAGNNNCTG 1 cut(s) 2366
PsuI RGATCY 3 cut(s) 55, 1484, 1502
RsaI GTAC 2 cut(s) 364, 1152
RsaNI GTAC 2 cut(s) 363, 1151
RseI CAYNNNNRTG 3 cut(s) 1714, 2313, 2368
SacI GAGCTC 1 cut(s) 2331
SapI GCTCTTC 1 cut(s) 2336
SaqAI TTAA 6 cut(s) 150, 252, 833, 1695, 2048, 2217
SatI GCNGC 3 cut(s) 753, 1869, 2361
Sau3AI GATC 9 cut(s) 55, 571, 895, 950, 1484, 1502, 1555, 2140, 2278
Sau96I GGNCC 3 cut(s) 670, 861, 1138
SchI GAGTC 4 cut(s) 14, 657, 751, 1843
ScrFI CCNGG 6 cut(s) 668, 881, 948, 1023, 1889, 2012
SduI GDGCHC 6 cut(s) 946, 1650, 1790, 1859, 2331, 2377
SfaNI GCATC 8 cut(s) 78, 81, 1018, 1068, 1186, 2116, 2182, 2305
SfcI CTRYAG 3 cut(s) 505, 1146, 1388
SinI GGWCC 3 cut(s) 670, 861, 1138
SmiMI CAYNNNNRTG 3 cut(s) 1714, 2313, 2368
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
SpeI ACTAGT 1 cut(s) 1220
SsiI CCGC 5 cut(s) 392, 563, 700, 2125, 2273
SspMI CTAG 6 cut(s) 608, 843, 1221, 1464, 1934, 2189
SstI GAGCTC 1 cut(s) 2331
StyD4I CCNGG 6 cut(s) 666, 879, 946, 1021, 1887, 2010
StyI CCWWGG 3 cut(s) 46, 607, 2301
TaaI ACNGT 9 cut(s) 509, 674, 1169, 1409, 1590, 1701, 1781, 1826, 2181
TaiI ACGT 3 cut(s) 712, 1318, 1451
TaqI TCGA 4 cut(s) 574, 768, 2166, 2284
TfiI GAWTC 5 cut(s) 337, 761, 1688, 2005, 2405
Tru1I TTAA 6 cut(s) 150, 252, 833, 1695, 2048, 2217
Tru9I TTAA 6 cut(s) 150, 252, 833, 1695, 2048, 2217
TseFI GTSAC 2 cut(s) 1820, 1883
TseI GCWGC 3 cut(s) 752, 1868, 2360
Tsp45I GTSAC 2 cut(s) 1820, 1883
TspDTI ATGAA 7 cut(s) 495, 567, 1101, 1127, 1394, 2190, 2397
TspGWI ACGGA 1 cut(s) 1630
VpaK11BI GGWCC 3 cut(s) 670, 861, 1138
XagI CCTNNNNNAGG 1 cut(s) 611
XapI RAATTY 6 cut(s) 41, 111, 214, 554, 576, 2219
XceI RCATGY 1 cut(s) 1474
XcmI CCANNNNNNNNNTGG 2 cut(s) 452, 1727
XmaJI CCTAGG 1 cut(s) 607
XmiI GTMKAC 2 cut(s) 504, 1703
XmnI GAANNNNTTC 1 cut(s) 1053
XspI CTAG 6 cut(s) 608, 843, 1221, 1464, 1934, 2189
Zsp2I ATGCAT 1 cut(s) 1476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.