Rroxscaffold_3G00219270

Histone-lysine N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005087
Physical Location & Seq
Reverse (-)
1419320 .. 1426160
6841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00219270.1

Sequence Viewer

Length: 2559 bp
ATGAGCGCAACAGCGCGAGGAGTGATGATGTCCAAAGCTGCCGACTCTGCAACTAAACTCAAAAGATCACATGGAGATGAATCAGGAGATACTGTAGGCTTGGCACAGAAGATGTATCAGCTTAAGAAGCAAATTCAAGCAGAGAGAGTGATGTCAGTGAAGGAGAAAATTGAGCAGAATAGGAAGAAGCTAGAAAGTTACGTTTCGGAAATATCGTCAGTAATTTCAAGAGAAAATGAGCCAAGTGGGAGCTCTAGATTGCTTAATTTAAGAATTCAACATCCTCCCTGCAGATTCAGTGGTTTTTCTAAGGGATTTGGTGATAAAGATTATAGTAATAATGAAGATGAGTTACTCTCATCAAGTATTGAGCTTCCAAAAGCTCACAAGATTGATCCTTATACAACTTGGATATGGTTGGACAGGAATCAGAGAATGGCTGAGGACCAATCAGTAGTTGGGAGGAGGCAAATTTACTACGATAAAGAACATGGCGTTGAAGCTCTAGTCTACAGTGACACTGATGATGAGATGACAGAACCTGAAGAAGTGAAACGTGCATTTTCTGGAGGAGAAGATCGAATTCTGTCGATGGCCTTTCAAGAGCATGGGACAGGAGAAGAAGTAATGAAAGCTGTGAGCCAGTTTATTGGAGCCACCACTTCGGAAATCCAGGAGCGGTACATTACAATTAAGGAGCGGGACTGTGAAAAACATGAATCAAAAGATTCTGGGGAATCTGGATCAGATAAGCACATTTCTCTTGATAAGAGTCTCAGTGCTGCATTAGATTCTTTTGATAACCTTTTCTGTCGCCGTTGCCTGATATTTGATTGTCGTCTGCATGGCTGTTCTCAACCTCTAATCTATCCTAGTGAAAAGCAGTTGCATTGGTCAGAACATGATGAGGACGACAAACCTTGCAGTGATCAATGTTACCTTAGGTTAAAGGCAGTTGACAACCCGTTGGAAGGTCCACATATTAATATAACAAAGTCAGAGAGAGAAAGTGCTCCAGCATCATCCTTTAATGCTGAACAACTAAGTTCTCGTTGTAGTATGGACATCATACATGATGAAAGATACATTCCTGGAAAATCTGTGGTTGTCACTTCAGAAACTATTCATAGTTCAGAAGTCAATACTGGAGCTTTAGGTTTGGATACAGATATGATGATGACTCACAATGAAAATTCAGGGAAACGGAAGGTCGTAATGTACACAGATAAAGTGGCACACGACCAAACTATAGTGCCGGATGATTTGCAGGGGTCTTGCAAGAAACAGAAGGGATTAGATGCCTTGCACTTAGTAACTAGTACACCAATCCTGGTTCAAGATCACTTTACCAGTTCTGAACTTAAGAACTCAGATGGTGATTTACCCAATAAAAATGAACTTGAAATGCCCAACAACGAATGCACCAGACATACTTCAAAGGAACTTGTTTGTTTTGGTAGTTCTTCCCGTGATGAATCTATGGAAGATATCAAAGATAAGCCAAAAGATGTAGTAGAAGTACTGAAGCAAACATCCAAATCTACAGGAGGGCAAGTTGAAGGGCAGTGTAGTAGCAGCTCTGAATGGAAGGCAGTAGAGAAAGAATTATACATGAAGGGATTAGAGATATTTGGGAGAAACAGTTGCCTTATAGCCAGGAACTTACTTTCTGGCTTCAGGACTTGCTTGGAAGTGTCCATGTTCATGCATGATGCTGAAGCTTCAATGCCTAACAGATCTGTTGATGGATTTATGGAAGACAACGGAAACGGAATAGCTGATACGGATCAGAGTGAACAAGAGATGCGGACAAAGTCACGATCATTTCGTAGAAGGGGCAAAGCACGGAGGCTTAAATATTCTTGGAAGTCTGCTGGTCACCCATCAGTCTGGAAAAGGATTGCTGATGGCAAAAACCAGTCCCGCAAACAATATATACCATGTGGATGCCAGTCTACATGTGGAAAGCAATGTTCTTATGCTCAAAGAGCTGCAAAAATAGGTTCAGGGGATGCCACTGTGCAAAAAGTCAATGCAGAAGTCGGCAGTGCCCATGCTTTGCCGCAGATCGTGAATGTGACCCAGATGTCTGTCGCAATTGTTGGGTTAGAAAGATATGTCAAATTGCTGCTGTATATGGACCAGCTGGTTGAGCTTTGTGGGGACAGTTCATTAGGCGAACCACCAAAACAGGGTGATAGTCAATGTGGAAACATGAGGCTTCTTCTAAGGCAACAACAAAGGATTCTCTTGGGTAAATCTGATGTTGCTGGATGGGGAGCCTTTTTAAAGTGGGTCCTAGATGCTTACCGAAAAGGAGACAAGCTTAAATTTGCAAACCACTCGAAGAAACCTAACTGTCACGCAAAGGTAATGCTGGTGGCGGGAGATCACCGAGTAGGCATATTTGCCAAGGAGCATATTGATGCTGGTGAGGAGATCTTCTATGATTATTGTTATCCACCAGAAACAGAAATCCCATGGGCTCAAGCTGAGGGTACAAAGAGGGATGATTCATCTGTCTCCCAAGTCAGAGCGAAGAAACACCAATCTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0003723 GO:0003727 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005677 GO:0005911 GO:0006139 GO:0006325 GO:0006342 GO:0006349 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008213 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009642 GO:0009646 GO:0009653 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0017053 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0030054 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031519 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048316 GO:0048317 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048587 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0055044 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070734 GO:0071514 GO:0071704 GO:0071840 GO:0080050 GO:0080090 GO:0090568 GO:0090698 GO:0097159 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:2000014 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00310 ko05206 map00310 map05206
Pfam Domains
Protein Families
SET

Protein Analysis

852

Amino Acids

95.47

Weight (kDa)

6.23

Isoelectric Point (pI)

46.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_CLF_N PF25996 188 - 232 3.2e-20 Histone-lysine N-methyltransferase CLF, N-terminal HTH domain
SET PF00856 755 - 818 9.9e-12 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2086
AccBSI CCGCTC 2 cut(s) 679, 700
AccI GTMKAC 2 cut(s) 510, 1955
AccII CGCG 1 cut(s) 16
AciI CCGC 6 cut(s) 679, 700, 1807, 1924, 2063, 2384
AclWI GGATC 3 cut(s) 389, 751, 1794
AcsI RAATTY 6 cut(s) 132, 273, 471, 582, 1192, 2330
AcuI CTGAAG 5 cut(s) 564, 1098, 1544, 1660, 1737
AfaI GTAC 5 cut(s) 683, 1220, 1321, 1521, 2500
AfiI CCNNNNNNNGG 2 cut(s) 971, 2192
AflII CTTAAG 2 cut(s) 122, 1361
AflIII ACRYGT 1 cut(s) 1958
AhlI ACTAGT 1 cut(s) 1316
AjnI CCWGG 4 cut(s) 672, 1090, 1329, 1655
Alw21I GWGCWC 2 cut(s) 254, 1015
Alw26I GTCTC 3 cut(s) 779, 2313, 2527
AlwI GGATC 3 cut(s) 389, 751, 1794
AlwNI CAGNNNCTG 1 cut(s) 542
AoxI GGCC 1 cut(s) 594
ApeKI GCWGC 5 cut(s) 38, 782, 1575, 1991, 2128
ApoI RAATTY 6 cut(s) 132, 273, 471, 582, 1192, 2330
AseI ATTAAT 1 cut(s) 984
Asp700I GAANNNNTTC 1 cut(s) 1122
AspLEI GCGC 2 cut(s) 8, 16
AspS9I GGNCC 4 cut(s) 445, 974, 2140, 2296
AsuHPI GGTGA 6 cut(s) 332, 1388, 1871, 2207, 2384, 2444
AvaII GGWCC 4 cut(s) 445, 974, 2140, 2296
AxyI CCTNAGG 1 cut(s) 941
BaeGI GKGCMC 1 cut(s) 2053
BanII GRGCYC 2 cut(s) 254, 2488
BarI GAAGNNNNNNTAC 2 cut(s) 336, 368
BbsI GAAGAC 1 cut(s) 1764
Bbv12I GWGCWC 2 cut(s) 254, 1015
BbvCI CCTCAGC 2 cut(s) 441, 2493
BbvI GCAGC 5 cut(s) 25, 769, 1587, 1978, 2115
BccI CCATC 6 cut(s) 586, 1367, 1739, 1891, 1901, 2268
BceAI ACGGC 1 cut(s) 801
BcgI CGANNNNNNTGC 2 cut(s) 2325, 2359
BciT130I CCWGG 4 cut(s) 674, 1092, 1331, 1657
BciVI GTATCC 1 cut(s) 1156
BclI TGATCA 1 cut(s) 928
BcoDI GTCTC 3 cut(s) 779, 2313, 2527
BcuI ACTAGT 1 cut(s) 1316
BfaI CTAG 6 cut(s) 191, 255, 506, 873, 1317, 2300
BfmI CTRYAG 5 cut(s) 93, 289, 511, 1248, 1542
BfrI CTTAAG 2 cut(s) 122, 1361
BfuI GTATCC 1 cut(s) 1156
BglII AGATCT 2 cut(s) 1736, 2439
BisI GCNGC 6 cut(s) 39, 783, 1576, 1992, 2063, 2129
BlsI GCNGC 6 cut(s) 40, 784, 1577, 1993, 2064, 2130
BmcAI AGTACT 1 cut(s) 1521
Bme1390I CCNGG 4 cut(s) 674, 1092, 1331, 1657
Bme18I GGWCC 4 cut(s) 445, 974, 2140, 2296
BmgT120I GGNCC 4 cut(s) 445, 974, 2140, 2296
BmiI GGNNCC 3 cut(s) 655, 2281, 2297
BmrFI CCNGG 4 cut(s) 674, 1092, 1331, 1657
BmsI GCATC 8 cut(s) 1028, 1288, 1702, 1794, 1937, 2002, 2293, 2416
BpiI GAAGAC 1 cut(s) 1764
BplI GAGNNNNNCTC 2 cut(s) 341, 373
BpmI CTGGAG 3 cut(s) 588, 999, 1167
Bpu10I CCTNAGC 2 cut(s) 441, 2493
BpuEI CTTGAG 1 cut(s) 2472
BsaBI GATNNNNATC 1 cut(s) 1785
BsaJI CCNNGG 2 cut(s) 2412, 2480
BsaXI ACNNNNNCTCC 8 cut(s) 78, 108, 609, 639, 1539, 1569, 1627, 1657
Bsc4I CCNNNNNNNGG 2 cut(s) 971, 2192
Bse1I ACTGG 5 cut(s) 643, 1150, 1350, 1918, 1951
Bse21I CCTNAGG 1 cut(s) 941
Bse3DI GCAATG 1 cut(s) 1976
Bse8I GATNNNNATC 1 cut(s) 1785
BseBI CCWGG 4 cut(s) 674, 1092, 1331, 1657
BseDI CCNNGG 2 cut(s) 2412, 2480
BseGI GGATG 8 cut(s) 280, 1022, 1264, 1532, 1952, 2017, 2279, 2515
BseJI GATNNNNATC 1 cut(s) 1785
BseLI CCNNNNNNNGG 2 cut(s) 971, 2192
BseMI GCAATG 1 cut(s) 1976
BseMII CTCAG 4 cut(s) 432, 790, 1383, 2484
BseNI ACTGG 5 cut(s) 643, 1150, 1350, 1918, 1951
BseRI GAGGAG 4 cut(s) 33, 478, 585, 2450
BseSI GKGCMC 1 cut(s) 2053
BseXI GCAGC 5 cut(s) 25, 769, 1587, 1978, 2115
Bsh1236I CGCG 1 cut(s) 16
BshFI GGCC 1 cut(s) 596
BsiHKAI GWGCWC 2 cut(s) 254, 1015
BsiSI CCGG 1 cut(s) 1256
BslFI GGGAC 4 cut(s) 625, 716, 1906, 2177
BslI CCNNNNNNNGG 2 cut(s) 971, 2192
BsmAI GTCTC 3 cut(s) 779, 2313, 2527
BsmFI GGGAC 4 cut(s) 625, 716, 1906, 2177
BsmI GAATGC 1 cut(s) 1424
BsnI GGCC 1 cut(s) 596
Bsp1286I GDGCHC 4 cut(s) 254, 1015, 2053, 2488
Bsp1407I TGTACA 1 cut(s) 1218
Bsp19I CCATGG 1 cut(s) 2480
BspACI CCGC 6 cut(s) 679, 700, 1807, 1924, 2063, 2384
BspANI GGCC 1 cut(s) 596
BspCNI CTCAG 4 cut(s) 433, 789, 1382, 2485
BspFNI CGCG 1 cut(s) 16
BspLI GGNNCC 3 cut(s) 655, 2281, 2297
BspMAI CTGCAG 1 cut(s) 293
BspPI GGATC 3 cut(s) 389, 751, 1794
BspTI CTTAAG 2 cut(s) 122, 1361
BsrBI CCGCTC 2 cut(s) 679, 700
BsrDI GCAATG 1 cut(s) 1976
BsrGI TGTACA 1 cut(s) 1218
BsrI ACTGG 5 cut(s) 643, 1150, 1350, 1918, 1951
BssECI CCNNGG 2 cut(s) 2412, 2480
BssT1I CCWWGG 2 cut(s) 2412, 2480
Bst2UI CCWGG 4 cut(s) 674, 1092, 1331, 1657
Bst4CI ACNGT 7 cut(s) 94, 515, 707, 1643, 2020, 2168, 2360
BstAFI CTTAAG 2 cut(s) 122, 1361
BstAUI TGTACA 1 cut(s) 1218
BstDEI CTNAG 9 cut(s) 309, 441, 776, 941, 1043, 1309, 1369, 2228, 2493
BstDSI CCRYGG 1 cut(s) 2480
BstEII GGTNACC 1 cut(s) 1877
BstF5I GGATG 8 cut(s) 280, 1022, 1264, 1532, 1952, 2017, 2279, 2515
BstFNI CGCG 1 cut(s) 16
BstHHI GCGC 2 cut(s) 8, 16
BstMAI GTCTC 3 cut(s) 779, 2313, 2527
BstMWI GCNNNNNNNGC 4 cut(s) 47, 127, 1988, 2152
BstNI CCWGG 4 cut(s) 674, 1092, 1331, 1657
BstNSI RCATGY 1 cut(s) 1962
BstPI GGTNACC 1 cut(s) 1877
BstSCI CCNGG 4 cut(s) 672, 1090, 1329, 1655
BstSFI CTRYAG 5 cut(s) 93, 289, 511, 1248, 1542
BstSLI GKGCMC 1 cut(s) 2053
BstUI CGCG 1 cut(s) 16
BstV1I GCAGC 5 cut(s) 25, 769, 1587, 1978, 2115
BstV2I GAAGAC 1 cut(s) 1764
BstX2I RGATCY 2 cut(s) 1736, 2439
BstXI CCANNNNNNTGG 2 cut(s) 650, 1890
BstYI RGATCY 2 cut(s) 1736, 2439
Bsu36I CCTNAGG 1 cut(s) 941
BsuI GTATCC 1 cut(s) 1156
BsuRI GGCC 1 cut(s) 596
BtgI CCRYGG 1 cut(s) 2480
BtsCI GGATG 8 cut(s) 280, 1022, 1264, 1532, 1952, 2017, 2279, 2515
BtsI GCAGTG 3 cut(s) 931, 1571, 2053
CaiI CAGNNNCTG 1 cut(s) 542
CfoI GCGC 2 cut(s) 8, 16
Cfr13I GGNCC 4 cut(s) 445, 974, 2140, 2296
Csp6I GTAC 5 cut(s) 682, 1219, 1320, 1520, 2499
CviQI GTAC 5 cut(s) 682, 1219, 1320, 1520, 2499
DdeI CTNAG 9 cut(s) 309, 441, 776, 941, 1043, 1309, 1369, 2228, 2493
DraI TTTAAA 1 cut(s) 2289
DrdI GACNNNNNNGTC 1 cut(s) 2086
DseDI GACNNNNNNGTC 1 cut(s) 2086
Ecl136II GAGCTC 1 cut(s) 252
Eco130I CCWWGG 2 cut(s) 2412, 2480
Eco24I GRGCYC 2 cut(s) 254, 2488
Eco32I GATATC 1 cut(s) 1489
Eco47I GGWCC 4 cut(s) 445, 974, 2140, 2296
Eco53kI GAGCTC 1 cut(s) 252
Eco57I CTGAAG 5 cut(s) 564, 1098, 1544, 1660, 1737
Eco81I CCTNAGG 1 cut(s) 941
Eco91I GGTNACC 1 cut(s) 1877
EcoICRI GAGCTC 1 cut(s) 252
EcoO109I RGGNCCY 1 cut(s) 2296
EcoO65I GGTNACC 1 cut(s) 1877
EcoRI GAATTC 2 cut(s) 273, 582
EcoRII CCWGG 4 cut(s) 672, 1090, 1329, 1655
EcoRV GATATC 1 cut(s) 1489
EcoT14I CCWWGG 2 cut(s) 2412, 2480
EcoT22I ATGCAT 1 cut(s) 1710
EcoT38I GRGCYC 2 cut(s) 254, 2488
ErhI CCWWGG 2 cut(s) 2412, 2480
FaqI GGGAC 4 cut(s) 625, 716, 1906, 2177
FauI CCCGC 3 cut(s) 693, 1931, 2377
FbaI TGATCA 1 cut(s) 928
FblI GTMKAC 2 cut(s) 510, 1955
Fnu4HI GCNGC 6 cut(s) 39, 783, 1576, 1992, 2063, 2129
FokI GGATG 8 cut(s) 267, 1009, 1271, 1519, 1959, 2024, 2286, 2522
FriOI GRGCYC 2 cut(s) 254, 2488
Fsp4HI GCNGC 6 cut(s) 39, 783, 1576, 1992, 2063, 2129
FspBI CTAG 6 cut(s) 191, 255, 506, 873, 1317, 2300
GlaI GCGC 2 cut(s) 7, 15
GluI GCNGC 6 cut(s) 39, 783, 1576, 1992, 2063, 2129
GsuI CTGGAG 3 cut(s) 588, 999, 1167
HaeIII GGCC 1 cut(s) 596
HapII CCGG 1 cut(s) 1256
HhaI GCGC 2 cut(s) 8, 16
Hin6I GCGC 2 cut(s) 6, 14
HinP1I GCGC 2 cut(s) 6, 14
HincII GTYRAC 1 cut(s) 958
HindII GTYRAC 1 cut(s) 958
HindIII AAGCTT 2 cut(s) 1719, 2324
HpaII CCGG 1 cut(s) 1256
HphI GGTGA 6 cut(s) 332, 1388, 1871, 2207, 2384, 2444
Hpy166II GTNNAC 7 cut(s) 511, 958, 977, 1221, 1322, 1796, 1956
Hpy8I GTNNAC 7 cut(s) 511, 958, 977, 1221, 1322, 1796, 1956
HpyAV CCTTC 8 cut(s) 154, 965, 1201, 1282, 1553, 1582, 1609, 1827
HpyCH4III ACNGT 7 cut(s) 94, 515, 707, 1643, 2020, 2168, 2360
HpyCH4IV ACGT 2 cut(s) 201, 556
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 127, 1988, 2152
HpyF3I CTNAG 9 cut(s) 309, 441, 776, 941, 1043, 1309, 1369, 2228, 2493
HpySE526I ACGT 2 cut(s) 201, 556
HspAI GCGC 2 cut(s) 6, 14
Ksp22I TGATCA 1 cut(s) 928
LmnI GCTCC 8 cut(s) 249, 653, 676, 697, 1018, 1148, 2279, 2416
Lsp1109I GCAGC 5 cut(s) 25, 769, 1587, 1978, 2115
LweI GCATC 8 cut(s) 1028, 1288, 1702, 1794, 1937, 2002, 2293, 2416
MaeI CTAG 6 cut(s) 191, 255, 506, 873, 1317, 2300
MaeII ACGT 2 cut(s) 201, 556
MbiI CCGCTC 2 cut(s) 679, 700
MfeI CAATTG 1 cut(s) 2097
MflI RGATCY 2 cut(s) 1736, 2439
MhlI GDGCHC 4 cut(s) 254, 1015, 2053, 2488
MlyI GAGTC 3 cut(s) 38, 781, 1174
MmeI TCCRAC 2 cut(s) 399, 948
Mph1103I ATGCAT 1 cut(s) 1710
MroXI GAANNNNTTC 1 cut(s) 1122
MslI CAYNNNNRTG 4 cut(s) 75, 1703, 1945, 2424
MspA1I CMGCKG 1 cut(s) 2146
MspCI CTTAAG 2 cut(s) 122, 1361
MspI CCGG 1 cut(s) 1256
MspR9I CCNGG 4 cut(s) 674, 1092, 1331, 1657
MunI CAATTG 1 cut(s) 2097
Mva1269I GAATGC 1 cut(s) 1424
MvaI CCWGG 4 cut(s) 674, 1092, 1331, 1657
MvnI CGCG 1 cut(s) 16
MwoI GCNNNNNNNGC 4 cut(s) 47, 127, 1988, 2152
NcoI CCATGG 1 cut(s) 2480
NlaIV GGNNCC 3 cut(s) 655, 2281, 2297
NmuCI GTSAC 6 cut(s) 515, 1108, 1815, 1877, 2077, 2360
NsiI ATGCAT 1 cut(s) 1710
NspI RCATGY 1 cut(s) 1962
PciI ACATGT 1 cut(s) 1958
PcsI WCGNNNNNNNCGW 2 cut(s) 212, 1825
PctI GAATGC 1 cut(s) 1424
PdmI GAANNNNTTC 1 cut(s) 1122
PflFI GACNNNGTC 1 cut(s) 1813
PfoI TCCNGGA 2 cut(s) 672, 1090
PkrI GCNGC 6 cut(s) 40, 784, 1577, 1993, 2064, 2130
PleI GAGTC 3 cut(s) 38, 780, 1174
PpsI GAGTC 3 cut(s) 38, 780, 1174
PpuMI RGGWCCY 1 cut(s) 2296
PscI ACATGT 1 cut(s) 1958
PshBI ATTAAT 1 cut(s) 984
Psp124BI GAGCTC 1 cut(s) 254
Psp5II RGGWCCY 1 cut(s) 2296
Psp6I CCWGG 4 cut(s) 672, 1090, 1329, 1655
PspEI GGTNACC 1 cut(s) 1877
PspGI CCWGG 4 cut(s) 672, 1090, 1329, 1655
PspN4I GGNNCC 3 cut(s) 655, 2281, 2297
PspPI GGNCC 4 cut(s) 445, 974, 2140, 2296
PspPPI RGGWCCY 1 cut(s) 2296
PstI CTGCAG 1 cut(s) 293
PstNI CAGNNNCTG 1 cut(s) 542
PsuI RGATCY 2 cut(s) 1736, 2439
PsyI GACNNNGTC 1 cut(s) 1813
PvuII CAGCTG 1 cut(s) 2146
RsaI GTAC 5 cut(s) 683, 1220, 1321, 1521, 2500
RsaNI GTAC 5 cut(s) 682, 1219, 1320, 1520, 2499
RseI CAYNNNNRTG 4 cut(s) 75, 1703, 1945, 2424
SacI GAGCTC 1 cut(s) 254
SatI GCNGC 6 cut(s) 39, 783, 1576, 1992, 2063, 2129
Sau96I GGNCC 4 cut(s) 445, 974, 2140, 2296
ScaI AGTACT 1 cut(s) 1521
SchI GAGTC 3 cut(s) 38, 781, 1174
ScrFI CCNGG 4 cut(s) 674, 1092, 1331, 1657
SduI GDGCHC 4 cut(s) 254, 1015, 2053, 2488
SfaNI GCATC 8 cut(s) 1028, 1288, 1702, 1794, 1937, 2002, 2293, 2416
SfcI CTRYAG 5 cut(s) 93, 289, 511, 1248, 1542
SinI GGWCC 4 cut(s) 445, 974, 2140, 2296
SmiMI CAYNNNNRTG 4 cut(s) 75, 1703, 1945, 2424
SmlI CTYRAG 3 cut(s) 122, 1361, 2487
SmoI CTYRAG 3 cut(s) 122, 1361, 2487
SpeI ACTAGT 1 cut(s) 1316
SsiI CCGC 6 cut(s) 679, 700, 1807, 1924, 2063, 2384
SspI AATATT 1 cut(s) 1859
SspMI CTAG 6 cut(s) 191, 255, 506, 873, 1317, 2300
SstI GAGCTC 1 cut(s) 254
StyD4I CCNGG 4 cut(s) 672, 1090, 1329, 1655
StyI CCWWGG 2 cut(s) 2412, 2480
TaaI ACNGT 7 cut(s) 94, 515, 707, 1643, 2020, 2168, 2360
TaiI ACGT 2 cut(s) 204, 559
TaqI TCGA 3 cut(s) 580, 590, 2345
TatI WGTACW 3 cut(s) 1218, 1319, 1519
TauI GCSGC 1 cut(s) 2065
TscAI CASTG 9 cut(s) 162, 304, 520, 526, 784, 931, 1571, 2023, 2053
TseFI GTSAC 6 cut(s) 515, 1108, 1815, 1877, 2077, 2360
TseI GCWGC 5 cut(s) 38, 782, 1575, 1991, 2128
Tsp45I GTSAC 6 cut(s) 515, 1108, 1815, 1877, 2077, 2360
TspGWI ACGGA 5 cut(s) 1219, 1779, 1785, 1799, 1861
TspRI CASTG 9 cut(s) 162, 304, 520, 526, 784, 931, 1571, 2023, 2053
Tth111I GACNNNGTC 1 cut(s) 1813
Vha464I CTTAAG 2 cut(s) 122, 1361
VpaK11BI GGWCC 4 cut(s) 445, 974, 2140, 2296
VspI ATTAAT 1 cut(s) 984
XapI RAATTY 6 cut(s) 132, 273, 471, 582, 1192, 2330
XbaI TCTAGA 1 cut(s) 254
XceI RCATGY 1 cut(s) 1962
XcmI CCANNNNNNNNNTGG 2 cut(s) 455, 1958
XmiI GTMKAC 2 cut(s) 510, 1955
XmnI GAANNNNTTC 1 cut(s) 1122
XspI CTAG 6 cut(s) 191, 255, 506, 873, 1317, 2300
ZrmI AGTACT 1 cut(s) 1521
Zsp2I ATGCAT 1 cut(s) 1710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.