MD04G1003900.v1.1

Belongs to the synaptobrevin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0017054
Physical Location & Seq
Reverse (-)
539323 .. 541964
2642 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1003900.v1.1.491

Sequence Viewer

Length: 663 bp
ATGGGGCAGCAATCTCTGATCTACAGCTTCGTCGCTCGAGGCTCTGTGATTCTCGCCGAGTACACAGAGTTCACTGGAAATTTCACCACCATCGCCGCTCAATGCCTCCAGAAGCTTCCTTCCTCCAACAACAAGTTCACCTACAATTGCGATGGCCACACCTTCAATTACCTCGTCGAAAACGGCTTCACCTACTGTGTAGTTGCTGCTGAATCTGCTGGGAGGCAAATTCCAATTGCCTACCTGGAGAGAATCAAGGATGATTTCAACAAAAGATATGGTGGTGGGAAAGCTGCGACTGCTGTTGCCAATGGACTGAACAGAGAGTTTGGACCCAAACTGAAGGACCACATGAAGTACTGTGTTGATCACCCTGAAGAGATCAACAAGCTTGCAAAAGTGAAGGCTCAGGTTACTGAGGTCAAGGGTGTTATGATGGAAAATATTGAGAAGGTTCTTGATCGCGGAGAGAAGATTGAGCTGTTGGTGGATAAAACTGATAATCTTCGCTCCCAGGCCCAAGATTTCAGGACACAGGGAACAAAAATGAAAAGAAAGATGTGGTTTCAGAACATGAAGATGAAACTGATTGTCGTGGGGATCCTCATTCTCATAGCCCTCATGATATATTTAACTATTTGCCGTGGTTTCAAGTGTACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
Longin Synaptobrevin

Protein Analysis

221

Amino Acids

24.92

Weight (kDa)

9.22

Isoelectric Point (pI)

34.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Longin PF13774 32 - 111 5.2e-23 Regulated-SNARE-like domain
Synaptobrevin PF00957 130 - 209 2.5e-30 Synaptobrevin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017054)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g00320
malus_domestica MD00G1099600.v1.1 MD04G1003900.v1.1
prunus_persica Prupe.1G003400_v2.0.a1
pyrus_communis pycom04g00400 pycom420g00810
rosa_laevigata RLG00000010253
rosa_roxburghii Rroxscaffold_5G00333070
rosa_rugosa Rorug03G0300000
rosa_samantha Rh4BG002600 Rh4CG003400 Rh4DG002900
rosa_wichuraiana Rw4G000610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 98
AccII CGCG 1 cut(s) 465
AciI CCGC 2 cut(s) 96, 465
AclWI GGATC 2 cut(s) 595, 608
AcoI YGGCCR 1 cut(s) 154
AcsI RAATTY 2 cut(s) 79, 228
AcuI CTGAAG 2 cut(s) 362, 396
AfaI GTAC 3 cut(s) 62, 359, 658
AgsI TTSAA 3 cut(s) 166, 268, 652
AjnI CCWGG 2 cut(s) 243, 513
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AluBI AGCT 5 cut(s) 27, 115, 293, 391, 481
AluI AGCT 5 cut(s) 27, 115, 293, 391, 481
AlwI GGATC 2 cut(s) 595, 608
Ama87I CYCGRG 1 cut(s) 36
AoxI GGCC 2 cut(s) 154, 516
ApeKI GCWGC 3 cut(s) 7, 206, 293
ApoI RAATTY 2 cut(s) 79, 228
AspS9I GGNCC 3 cut(s) 332, 346, 517
AsuHPI GGTGA 4 cut(s) 76, 130, 181, 362
AvaI CYCGRG 1 cut(s) 36
AvaII GGWCC 2 cut(s) 332, 346
BalI TGGCCA 1 cut(s) 156
BamHI GGATCC 1 cut(s) 600
BbvI GCAGC 3 cut(s) 19, 193, 280
BccI CCATC 3 cut(s) 98, 146, 430
BceAI ACGGC 2 cut(s) 199, 627
BciT130I CCWGG 2 cut(s) 245, 515
BclI TGATCA 1 cut(s) 367
BfmI CTRYAG 1 cut(s) 22
BisI GCNGC 4 cut(s) 8, 96, 207, 294
BlsI GCNGC 4 cut(s) 9, 97, 208, 295
BmcAI AGTACT 1 cut(s) 359
Bme1390I CCNGG 2 cut(s) 245, 515
Bme18I GGWCC 2 cut(s) 332, 346
BmeT110I CYCGRG 1 cut(s) 36
BmgT120I GGNCC 3 cut(s) 332, 346, 517
BmiI GGNNCC 2 cut(s) 334, 602
BmrFI CCNGG 2 cut(s) 245, 515
BpmI CTGGAG 2 cut(s) 92, 266
Bpu10I CCTNAGC 1 cut(s) 408
BsaJI CCNNGG 2 cut(s) 513, 643
Bse1I ACTGG 1 cut(s) 79
BseBI CCWGG 2 cut(s) 245, 515
BseDI CCNNGG 2 cut(s) 513, 643
BseGI GGATG 1 cut(s) 265
BseMII CTCAG 2 cut(s) 408, 422
BseNI ACTGG 1 cut(s) 79
BseXI GCAGC 3 cut(s) 19, 193, 280
BseYI CCCAGC 1 cut(s) 218
Bsh1236I CGCG 1 cut(s) 465
BshFI GGCC 2 cut(s) 156, 518
BsiHKCI CYCGRG 1 cut(s) 36
BsnI GGCC 2 cut(s) 156, 518
BsoBI CYCGRG 1 cut(s) 36
Bsp143I GATC 5 cut(s) 18, 367, 381, 460, 600
BspACI CCGC 2 cut(s) 96, 465
BspANI GGCC 2 cut(s) 156, 518
BspCNI CTCAG 2 cut(s) 409, 421
BspFNI CGCG 1 cut(s) 465
BspHI TCATGA 1 cut(s) 621
BspLI GGNNCC 2 cut(s) 334, 602
BspPI GGATC 2 cut(s) 595, 608
BsrBI CCGCTC 1 cut(s) 98
BsrI ACTGG 1 cut(s) 79
BssECI CCNNGG 2 cut(s) 513, 643
BssMI GATC 5 cut(s) 18, 367, 381, 460, 600
Bst2UI CCWGG 2 cut(s) 245, 515
Bst4CI ACNGT 2 cut(s) 197, 362
Bst6I CTCTTC 1 cut(s) 372
BstC8I GCNNGC 1 cut(s) 393
BstDEI CTNAG 2 cut(s) 408, 417
BstDSI CCRYGG 1 cut(s) 643
BstF5I GGATG 1 cut(s) 265
BstFNI CGCG 1 cut(s) 465
BstKTI GATC 5 cut(s) 21, 370, 384, 463, 603
BstMBI GATC 5 cut(s) 18, 367, 381, 460, 600
BstMWI GCNNNNNNNGC 2 cut(s) 215, 299
BstNI CCWGG 2 cut(s) 245, 515
BstSCI CCNGG 2 cut(s) 243, 513
BstSFI CTRYAG 1 cut(s) 22
BstUI CGCG 1 cut(s) 465
BstV1I GCAGC 3 cut(s) 19, 193, 280
BstX2I RGATCY 1 cut(s) 600
BstYI RGATCY 1 cut(s) 600
BsuRI GGCC 2 cut(s) 156, 518
BtgI CCRYGG 1 cut(s) 643
BtgZI GCGATG 2 cut(s) 76, 165
BtsCI GGATG 1 cut(s) 265
BtsIMutI CAGTG 1 cut(s) 72
Cac8I GCNNGC 1 cut(s) 393
CciI TCATGA 1 cut(s) 621
Cfr13I GGNCC 3 cut(s) 332, 346, 517
Csp6I GTAC 3 cut(s) 61, 358, 657
CviAII CATG 3 cut(s) 352, 574, 622
CviQI GTAC 3 cut(s) 61, 358, 657
DdeI CTNAG 2 cut(s) 408, 417
DpnI GATC 5 cut(s) 20, 369, 383, 462, 602
DpnII GATC 5 cut(s) 18, 367, 381, 460, 600
EaeI YGGCCR 1 cut(s) 154
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
Eco47I GGWCC 2 cut(s) 332, 346
Eco57I CTGAAG 2 cut(s) 362, 396
Eco88I CYCGRG 1 cut(s) 36
EcoRII CCWGG 2 cut(s) 243, 513
FaeI CATG 3 cut(s) 355, 577, 625
FaiI YATR 7 cut(s) 279, 353, 434, 575, 614, 623, 628
FatI CATG 3 cut(s) 351, 573, 621
FbaI TGATCA 1 cut(s) 367
Fnu4HI GCNGC 4 cut(s) 8, 96, 207, 294
FokI GGATG 1 cut(s) 272
Fsp4HI GCNGC 4 cut(s) 8, 96, 207, 294
GluI GCNGC 4 cut(s) 8, 96, 207, 294
GsaI CCCAGC 1 cut(s) 222
GsuI CTGGAG 2 cut(s) 92, 266
HaeIII GGCC 2 cut(s) 156, 518
Hin1II CATG 3 cut(s) 355, 577, 625
HindIII AAGCTT 2 cut(s) 113, 389
HinfI GANTC 3 cut(s) 49, 212, 252
HphI GGTGA 4 cut(s) 76, 130, 181, 362
Hpy166II GTNNAC 4 cut(s) 63, 72, 138, 657
Hpy188I TCNGA 2 cut(s) 18, 570
Hpy188III TCNNGA 4 cut(s) 109, 458, 529, 622
Hpy8I GTNNAC 4 cut(s) 63, 72, 138, 657
Hpy99I CGWCG 2 cut(s) 35, 179
HpyAV CCTTC 5 cut(s) 129, 172, 337, 397, 445
HpyCH4III ACNGT 2 cut(s) 197, 362
HpyCH4V TGCA 1 cut(s) 395
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 299
HpyF3I CTNAG 2 cut(s) 408, 417
Hsp92II CATG 3 cut(s) 355, 577, 625
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 5 cut(s) 18, 367, 381, 460, 600
LmnI GCTCC 1 cut(s) 515
Lsp1109I GCAGC 3 cut(s) 19, 193, 280
MaeIII GTNAC 1 cut(s) 412
MalI GATC 5 cut(s) 20, 369, 383, 462, 602
MbiI CCGCTC 1 cut(s) 98
MboI GATC 5 cut(s) 18, 367, 381, 460, 600
MboII GAAGA 4 cut(s) 389, 484, 497, 589
MfeI CAATTG 2 cut(s) 145, 234
MflI RGATCY 1 cut(s) 600
MlsI TGGCCA 1 cut(s) 156
MluCI AATT 5 cut(s) 79, 145, 166, 228, 234
MluNI TGGCCA 1 cut(s) 156
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 8 cut(s) 32, 116, 133, 182, 216, 412, 614, 629
Mox20I TGGCCA 1 cut(s) 156
MscI TGGCCA 1 cut(s) 156
MseI TTAA 1 cut(s) 632
MslI CAYNNNNRTG 1 cut(s) 578
Msp20I TGGCCA 1 cut(s) 156
MspR9I CCNGG 2 cut(s) 245, 515
MunI CAATTG 2 cut(s) 145, 234
MvaI CCWGG 2 cut(s) 245, 515
MvnI CGCG 1 cut(s) 465
MwoI GCNNNNNNNGC 2 cut(s) 215, 299
NdeII GATC 5 cut(s) 18, 367, 381, 460, 600
NlaIII CATG 3 cut(s) 355, 577, 625
NlaIV GGNNCC 2 cut(s) 334, 602
NmeAIII GCCGAG 1 cut(s) 82
PaeR7I CTCGAG 1 cut(s) 36
PagI TCATGA 1 cut(s) 621
PfeI GAWTC 3 cut(s) 49, 212, 252
PkrI GCNGC 4 cut(s) 9, 97, 208, 295
Psp6I CCWGG 2 cut(s) 243, 513
PspFI CCCAGC 1 cut(s) 218
PspGI CCWGG 2 cut(s) 243, 513
PspN4I GGNNCC 2 cut(s) 334, 602
PspPI GGNCC 3 cut(s) 332, 346, 517
PspXI VCTCGAGB 1 cut(s) 36
PsrI GAACNNNNNNTAC 2 cut(s) 53, 85
PsuI RGATCY 1 cut(s) 600
RsaI GTAC 3 cut(s) 62, 359, 658
RsaNI GTAC 3 cut(s) 61, 358, 657
RseI CAYNNNNRTG 1 cut(s) 578
SaqAI TTAA 1 cut(s) 632
SatI GCNGC 4 cut(s) 8, 96, 207, 294
Sau3AI GATC 5 cut(s) 18, 367, 381, 460, 600
Sau96I GGNCC 3 cut(s) 332, 346, 517
ScaI AGTACT 1 cut(s) 359
ScrFI CCNGG 2 cut(s) 245, 515
SfcI CTRYAG 1 cut(s) 22
Sfr274I CTCGAG 1 cut(s) 36
SinI GGWCC 2 cut(s) 332, 346
SlaI CTCGAG 1 cut(s) 36
SmiMI CAYNNNNRTG 1 cut(s) 578
SmlI CTYRAG 1 cut(s) 36
SmoI CTYRAG 1 cut(s) 36
Sse9I AATT 5 cut(s) 79, 145, 166, 228, 234
SsiI CCGC 2 cut(s) 96, 465
SspI AATATT 1 cut(s) 445
StyD4I CCNGG 2 cut(s) 243, 513
TaaI ACNGT 2 cut(s) 197, 362
TaqI TCGA 2 cut(s) 37, 177
TasI AATT 5 cut(s) 79, 145, 166, 228, 234
TatI WGTACW 2 cut(s) 60, 357
TauI GCSGC 1 cut(s) 98
TfiI GAWTC 3 cut(s) 49, 212, 252
Tru1I TTAA 1 cut(s) 632
Tru9I TTAA 1 cut(s) 632
TscAI CASTG 1 cut(s) 79
TseI GCWGC 3 cut(s) 7, 206, 293
TspDTI ATGAA 4 cut(s) 368, 563, 590, 596
TspRI CASTG 1 cut(s) 79
VpaK11BI GGWCC 2 cut(s) 332, 346
XapI RAATTY 2 cut(s) 79, 228
XhoI CTCGAG 1 cut(s) 36
ZrmI AGTACT 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.