RLG00000010253

Belongs to the synaptobrevin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0017054
Physical Location & Seq
Forward (+)
64692350 .. 64695048
2699 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010253

Sequence Viewer

Length: 669 bp
ATGGGGCAGCAATCTCTGATCTATAGCTTCGTGGCTCGAGGCACTGTGATTCTTGCCGAGTACACAGAGTTCACCGGTAATTTCACCAGCATCGCCTCTCAATGCCTCCAGAAGCTTCCTGCCTCCAACAACAAGTTCACCTACAATTGCGATGGCCACACCTTCAATTACCTCGTCGAAAACGGCTTCACCTACTGTGTAGTTGCAGCTGAATCTGCAGGTAGGCAAATTCCCATTGCCTATCTGGAGAGAATCAAGGATGATTTCAACAAAAGATATGCTGGTGGAAAAGCAGGGACTGCAACAGCCAATGGACTGAACAGAGAGTTTGGACCTAAGCTGAAGGAGCACATGAAGTACTGTGTGGATCATCCTGAAGAGATCAACAAGCTTGCAAAAGTGAAGGCTCAGGTTACTGAGGTCAAGGGTGTTATGATGGAAAATATTGAGAAGGTTCTTGACCGTGGTGAGAAGATTGAGCTGTTGGTGGATAAAACTGATAATCTCCGCTCTCAGGCCCAAGATTTCAAGCAACAAGGAACAAAAATGAGAAGGAAGATGTGGTTTCAGAATATGAAGATGAAGTTGACTGTTGTGGGGATTGTTGTTGTCTTAGGCCTCGTCATATTTTTGTCTGCTTGCAATGGTTTCAAGTGTGTTTCAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
Longin Synaptobrevin

Protein Analysis

223

Amino Acids

24.95

Weight (kDa)

9.04

Isoelectric Point (pI)

34.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Longin PF13774 32 - 111 1.7e-22 Regulated-SNARE-like domain
Synaptobrevin PF00957 130 - 210 3.6e-30 Synaptobrevin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017054)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g00320
malus_domestica MD00G1099600.v1.1 MD04G1003900.v1.1
prunus_persica Prupe.1G003400_v2.0.a1
pyrus_communis pycom04g00400 pycom420g00810
rosa_laevigata RLG00000010253
rosa_roxburghii Rroxscaffold_5G00333070
rosa_rugosa Rorug03G0300000
rosa_samantha Rh4BG002600 Rh4CG003400 Rh4DG002900
rosa_wichuraiana Rw4G000610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 209
AccBSI CCGCTC 1 cut(s) 510
AciI CCGC 1 cut(s) 508
AclWI GGATC 1 cut(s) 375
AcoI YGGCCR 1 cut(s) 154
AcsI RAATTY 1 cut(s) 228
AcuI CTGAAG 2 cut(s) 362, 396
AfaI GTAC 2 cut(s) 62, 359
AfiI CCNNNNNNNGG 1 cut(s) 514
AgeI ACCGGT 1 cut(s) 74
AgsI TTSAA 4 cut(s) 166, 268, 529, 652
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AluBI AGCT 6 cut(s) 27, 115, 209, 340, 391, 481
AluI AGCT 6 cut(s) 27, 115, 209, 340, 391, 481
Alw21I GWGCWC 1 cut(s) 351
AlwI GGATC 1 cut(s) 375
AlwNI CAGNNNCTG 1 cut(s) 299
Ama87I CYCGRG 1 cut(s) 36
AoxI GGCC 3 cut(s) 154, 516, 616
ApeKI GCWGC 2 cut(s) 7, 206
ApoI RAATTY 1 cut(s) 228
AsiGI ACCGGT 1 cut(s) 74
AspS9I GGNCC 2 cut(s) 332, 517
AsuHPI GGTGA 5 cut(s) 64, 76, 130, 181, 479
AvaI CYCGRG 1 cut(s) 36
AvaII GGWCC 1 cut(s) 332
BalI TGGCCA 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 351
BbvI GCAGC 2 cut(s) 19, 218
BccI CCATC 2 cut(s) 146, 430
BceAI ACGGC 1 cut(s) 199
BfmI CTRYAG 2 cut(s) 22, 216
BfuAI ACCTGC 1 cut(s) 209
BisI GCNGC 2 cut(s) 8, 207
BlsI GCNGC 2 cut(s) 9, 208
BmcAI AGTACT 1 cut(s) 359
Bme18I GGWCC 1 cut(s) 332
BmeT110I CYCGRG 1 cut(s) 36
BmgT120I GGNCC 2 cut(s) 332, 517
BmsI GCATC 1 cut(s) 99
BpmI CTGGAG 2 cut(s) 92, 266
Bpu10I CCTNAGC 2 cut(s) 336, 408
BsaJI CCNNGG 1 cut(s) 463
BsaWI WCCGGW 1 cut(s) 74
Bsc4I CCNNNNNNNGG 1 cut(s) 514
Bse118I RCCGGY 1 cut(s) 74
Bse3DI GCAATG 2 cut(s) 234, 649
BseDI CCNNGG 1 cut(s) 463
BseGI GGATG 2 cut(s) 265, 370
BseLI CCNNNNNNNGG 1 cut(s) 514
BseMI GCAATG 2 cut(s) 234, 649
BseMII CTCAG 3 cut(s) 408, 422, 527
BseXI GCAGC 2 cut(s) 19, 218
BshFI GGCC 3 cut(s) 156, 518, 618
BshTI ACCGGT 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 351
BsiHKCI CYCGRG 1 cut(s) 36
BsiSI CCGG 1 cut(s) 75
BslFI GGGAC 1 cut(s) 310
BslI CCNNNNNNNGG 1 cut(s) 514
BsmFI GGGAC 1 cut(s) 310
BsnI GGCC 3 cut(s) 156, 518, 618
BsoBI CYCGRG 1 cut(s) 36
Bsp1286I GDGCHC 1 cut(s) 351
Bsp143I GATC 3 cut(s) 18, 367, 381
BspACI CCGC 1 cut(s) 508
BspANI GGCC 3 cut(s) 156, 518, 618
BspCNI CTCAG 3 cut(s) 409, 421, 526
BspMAI CTGCAG 1 cut(s) 220
BspMI ACCTGC 1 cut(s) 209
BspPI GGATC 1 cut(s) 375
BsrBI CCGCTC 1 cut(s) 510
BsrDI GCAATG 2 cut(s) 234, 649
BsrFI RCCGGY 1 cut(s) 74
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 1 cut(s) 463
BssMI GATC 3 cut(s) 18, 367, 381
Bst4CI ACNGT 5 cut(s) 46, 197, 362, 464, 592
Bst6I CTCTTC 1 cut(s) 372
BstAPI GCANNNNNTGC 1 cut(s) 299
BstC8I GCNNGC 2 cut(s) 393, 640
BstDEI CTNAG 5 cut(s) 336, 408, 417, 513, 613
BstDSI CCRYGG 1 cut(s) 463
BstF5I GGATG 2 cut(s) 265, 370
BstKTI GATC 3 cut(s) 21, 370, 384
BstMBI GATC 3 cut(s) 18, 367, 381
BstMWI GCNNNNNNNGC 3 cut(s) 215, 299, 346
BstSFI CTRYAG 2 cut(s) 22, 216
BstV1I GCAGC 2 cut(s) 19, 218
BsuRI GGCC 3 cut(s) 156, 518, 618
BtgI CCRYGG 1 cut(s) 463
BtgZI GCGATG 2 cut(s) 76, 165
BtsCI GGATG 2 cut(s) 265, 370
BtsIMutI CAGTG 1 cut(s) 42
BveI ACCTGC 1 cut(s) 209
Cac8I GCNNGC 2 cut(s) 393, 640
CaiI CAGNNNCTG 1 cut(s) 299
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 2 cut(s) 332, 517
Csp6I GTAC 2 cut(s) 61, 358
CspAI ACCGGT 1 cut(s) 74
CviAII CATG 1 cut(s) 352
CviQI GTAC 2 cut(s) 61, 358
DdeI CTNAG 5 cut(s) 336, 408, 417, 513, 613
DpnI GATC 3 cut(s) 20, 369, 383
DpnII GATC 3 cut(s) 18, 367, 381
EaeI YGGCCR 1 cut(s) 154
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
Eco147I AGGCCT 1 cut(s) 618
Eco47I GGWCC 1 cut(s) 332
Eco57I CTGAAG 2 cut(s) 362, 396
Eco88I CYCGRG 1 cut(s) 36
FaeI CATG 1 cut(s) 355
FaiI YATR 6 cut(s) 24, 279, 353, 434, 575, 626
FaqI GGGAC 1 cut(s) 310
FatI CATG 1 cut(s) 351
Fnu4HI GCNGC 2 cut(s) 8, 207
FokI GGATG 2 cut(s) 272, 357
Fsp4HI GCNGC 2 cut(s) 8, 207
GluI GCNGC 2 cut(s) 8, 207
GsuI CTGGAG 2 cut(s) 92, 266
HaeIII GGCC 3 cut(s) 156, 518, 618
HapII CCGG 1 cut(s) 75
Hin1II CATG 1 cut(s) 355
HincII GTYRAC 1 cut(s) 588
HindII GTYRAC 1 cut(s) 588
HindIII AAGCTT 2 cut(s) 113, 389
HinfI GANTC 3 cut(s) 49, 212, 252
HpaII CCGG 1 cut(s) 75
HphI GGTGA 5 cut(s) 64, 76, 130, 181, 479
Hpy166II GTNNAC 4 cut(s) 63, 72, 138, 588
Hpy188I TCNGA 3 cut(s) 18, 570, 664
Hpy188III TCNNGA 4 cut(s) 109, 245, 374, 458
Hpy8I GTNNAC 4 cut(s) 63, 72, 138, 588
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 5 cut(s) 172, 337, 397, 445, 546
HpyCH4III ACNGT 5 cut(s) 46, 197, 362, 464, 592
HpyCH4V TGCA 5 cut(s) 206, 218, 302, 395, 642
HpyF10VI GCNNNNNNNGC 3 cut(s) 215, 299, 346
HpyF3I CTNAG 5 cut(s) 336, 408, 417, 513, 613
Hsp92II CATG 1 cut(s) 355
Kzo9I GATC 3 cut(s) 18, 367, 381
LmnI GCTCC 1 cut(s) 346
Lsp1109I GCAGC 2 cut(s) 19, 218
LweI GCATC 1 cut(s) 99
MaeIII GTNAC 1 cut(s) 412
MalI GATC 3 cut(s) 20, 369, 383
MbiI CCGCTC 1 cut(s) 510
MboI GATC 3 cut(s) 18, 367, 381
MboII GAAGA 4 cut(s) 389, 484, 568, 589
MfeI CAATTG 1 cut(s) 145
MhlI GDGCHC 1 cut(s) 351
MlsI TGGCCA 1 cut(s) 156
MluCI AATT 4 cut(s) 79, 145, 166, 228
MluNI TGGCCA 1 cut(s) 156
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 7 cut(s) 32, 106, 116, 133, 182, 412, 629
Mox20I TGGCCA 1 cut(s) 156
MscI TGGCCA 1 cut(s) 156
MseI TTAA 1 cut(s) 667
Msp20I TGGCCA 1 cut(s) 156
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 1 cut(s) 75
MunI CAATTG 1 cut(s) 145
MwoI GCNNNNNNNGC 3 cut(s) 215, 299, 346
NdeII GATC 3 cut(s) 18, 367, 381
NlaIII CATG 1 cut(s) 355
NmeAIII GCCGAG 1 cut(s) 82
PaeR7I CTCGAG 1 cut(s) 36
PceI AGGCCT 1 cut(s) 618
PfeI GAWTC 3 cut(s) 49, 212, 252
PinAI ACCGGT 1 cut(s) 74
PkrI GCNGC 2 cut(s) 9, 208
PspPI GGNCC 2 cut(s) 332, 517
PspXI VCTCGAGB 1 cut(s) 36
PsrI GAACNNNNNNTAC 2 cut(s) 53, 85
PstI CTGCAG 1 cut(s) 220
PstNI CAGNNNCTG 1 cut(s) 299
PvuII CAGCTG 1 cut(s) 209
RsaI GTAC 2 cut(s) 62, 359
RsaNI GTAC 2 cut(s) 61, 358
SaqAI TTAA 1 cut(s) 667
SatI GCNGC 2 cut(s) 8, 207
Sau3AI GATC 3 cut(s) 18, 367, 381
Sau96I GGNCC 2 cut(s) 332, 517
ScaI AGTACT 1 cut(s) 359
SduI GDGCHC 1 cut(s) 351
SfaNI GCATC 1 cut(s) 99
SfcI CTRYAG 2 cut(s) 22, 216
Sfr274I CTCGAG 1 cut(s) 36
SinI GGWCC 1 cut(s) 332
SlaI CTCGAG 1 cut(s) 36
SmlI CTYRAG 1 cut(s) 36
SmoI CTYRAG 1 cut(s) 36
Sse9I AATT 4 cut(s) 79, 145, 166, 228
SseBI AGGCCT 1 cut(s) 618
SsiI CCGC 1 cut(s) 508
SspI AATATT 1 cut(s) 445
StuI AGGCCT 1 cut(s) 618
TaaI ACNGT 5 cut(s) 46, 197, 362, 464, 592
TaqI TCGA 2 cut(s) 37, 177
TasI AATT 4 cut(s) 79, 145, 166, 228
TatI WGTACW 2 cut(s) 60, 357
TfiI GAWTC 3 cut(s) 49, 212, 252
Tru1I TTAA 1 cut(s) 667
Tru9I TTAA 1 cut(s) 667
TscAI CASTG 1 cut(s) 49
TseI GCWGC 2 cut(s) 7, 206
TspDTI ATGAA 3 cut(s) 368, 590, 596
TspRI CASTG 1 cut(s) 49
VpaK11BI GGWCC 1 cut(s) 332
XapI RAATTY 1 cut(s) 228
XcmI CCANNNNNNNNNTGG 1 cut(s) 241
XhoI CTCGAG 1 cut(s) 36
ZrmI AGTACT 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.