pycom420g00810

Regulated-SNARE-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_420
OG0017054
Physical Location & Seq
Reverse (-)
997134 .. 997406
273 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom420g00810.1

Sequence Viewer

Length: 273 bp
ATGGGGCAGCAATATCTGATCTACAGCTTCGTCGCTCGAGGCTCTATGGTTCTCGCCGAGTACACAGAGTTCACCGGAAATTTCACCACCATCGCCGCTCAATGCCTCCAGAAGCTTCCTTCCTCCAACAACAAGTTCACATACAATTGCGACGGCCACACCTTCAATTACCTTGTCGAAAACGGCTTCAGTGAGTCCCCATGCATTCATTTCCTCATGCGTTTTTTCGTTTCCTTCCCATTAATTTATGAGTTTCCCTTCAGTTTTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
Longin Synaptobrevin

Protein Analysis

91

Amino Acids

10.37

Weight (kDa)

5.46

Isoelectric Point (pI)

34.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Longin PF13774 32 - 63 3.5e-06 Regulated-SNARE-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017054)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g00320
malus_domestica MD00G1099600.v1.1 MD04G1003900.v1.1
prunus_persica Prupe.1G003400_v2.0.a1
pyrus_communis pycom04g00400 pycom420g00810
rosa_laevigata RLG00000010253
rosa_roxburghii Rroxscaffold_5G00333070
rosa_rugosa Rorug03G0300000
rosa_samantha Rh4BG002600 Rh4CG003400 Rh4DG002900
rosa_wichuraiana Rw4G000610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 98
AciI CCGC 1 cut(s) 96
AcoI YGGCCR 1 cut(s) 154
AcsI RAATTY 1 cut(s) 79
AcuI CTGAAG 2 cut(s) 172, 244
AfaI GTAC 1 cut(s) 62
AgsI TTSAA 1 cut(s) 166
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AluBI AGCT 2 cut(s) 27, 115
AluI AGCT 2 cut(s) 27, 115
Ama87I CYCGRG 1 cut(s) 36
AoxI GGCC 1 cut(s) 154
ApeKI GCWGC 1 cut(s) 7
ApoI RAATTY 1 cut(s) 79
AseI ATTAAT 1 cut(s) 242
AsuHPI GGTGA 2 cut(s) 64, 76
AvaI CYCGRG 1 cut(s) 36
BbvI GCAGC 1 cut(s) 19
BccI CCATC 1 cut(s) 98
BceAI ACGGC 2 cut(s) 169, 199
BfmI CTRYAG 1 cut(s) 22
BisI GCNGC 2 cut(s) 8, 96
BlsI GCNGC 2 cut(s) 9, 97
BmeT110I CYCGRG 1 cut(s) 36
BpmI CTGGAG 1 cut(s) 92
BsaWI WCCGGW 1 cut(s) 74
BseXI GCAGC 1 cut(s) 19
BshFI GGCC 1 cut(s) 156
BsiHKCI CYCGRG 1 cut(s) 36
BsiSI CCGG 1 cut(s) 75
BslFI GGGAC 1 cut(s) 181
BsmFI GGGAC 1 cut(s) 181
BsmI GAATGC 1 cut(s) 204
BsnI GGCC 1 cut(s) 156
BsoBI CYCGRG 1 cut(s) 36
Bsp143I GATC 1 cut(s) 18
BspACI CCGC 1 cut(s) 96
BspANI GGCC 1 cut(s) 156
BsrBI CCGCTC 1 cut(s) 98
BssMI GATC 1 cut(s) 18
BstKTI GATC 1 cut(s) 21
BstMBI GATC 1 cut(s) 18
BstSFI CTRYAG 1 cut(s) 22
BstV1I GCAGC 1 cut(s) 19
BsuRI GGCC 1 cut(s) 156
BtgZI GCGATG 1 cut(s) 76
BtsIMutI CAGTG 1 cut(s) 196
Csp6I GTAC 1 cut(s) 61
CviAII CATG 2 cut(s) 201, 217
CviJI RGCY 5 cut(s) 27, 42, 115, 156, 186
CviKI_1 RGCY 5 cut(s) 27, 42, 115, 156, 186
CviQI GTAC 1 cut(s) 61
DpnI GATC 1 cut(s) 20
DpnII GATC 1 cut(s) 18
EaeI YGGCCR 1 cut(s) 154
Eco57I CTGAAG 2 cut(s) 172, 244
Eco88I CYCGRG 1 cut(s) 36
EcoT22I ATGCAT 1 cut(s) 206
FaeI CATG 2 cut(s) 204, 220
FaiI YATR 5 cut(s) 47, 142, 202, 218, 249
FaqI GGGAC 1 cut(s) 181
FatI CATG 2 cut(s) 200, 216
Fnu4HI GCNGC 2 cut(s) 8, 96
Fsp4HI GCNGC 2 cut(s) 8, 96
GluI GCNGC 2 cut(s) 8, 96
GsuI CTGGAG 1 cut(s) 92
HaeIII GGCC 1 cut(s) 156
HapII CCGG 1 cut(s) 75
Hin1II CATG 2 cut(s) 204, 220
HindIII AAGCTT 1 cut(s) 113
HinfI GANTC 1 cut(s) 194
HpaII CCGG 1 cut(s) 75
HphI GGTGA 2 cut(s) 64, 76
Hpy166II GTNNAC 3 cut(s) 63, 72, 138
Hpy188I TCNGA 1 cut(s) 18
Hpy188III TCNNGA 1 cut(s) 109
Hpy8I GTNNAC 3 cut(s) 63, 72, 138
Hpy99I CGWCG 2 cut(s) 35, 155
HpyAV CCTTC 4 cut(s) 129, 172, 244, 268
HpyCH4V TGCA 1 cut(s) 204
Hsp92II CATG 2 cut(s) 204, 220
Kzo9I GATC 1 cut(s) 18
LpnPI CCDG 2 cut(s) 88, 122
Lsp1109I GCAGC 1 cut(s) 19
MalI GATC 1 cut(s) 20
MbiI CCGCTC 1 cut(s) 98
MboI GATC 1 cut(s) 18
MfeI CAATTG 1 cut(s) 145
MluCI AATT 4 cut(s) 79, 145, 166, 243
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 4 cut(s) 32, 116, 133, 224
Mph1103I ATGCAT 1 cut(s) 206
MseI TTAA 1 cut(s) 242
MspI CCGG 1 cut(s) 75
MunI CAATTG 1 cut(s) 145
Mva1269I GAATGC 1 cut(s) 204
NdeII GATC 1 cut(s) 18
NlaIII CATG 2 cut(s) 204, 220
NmeAIII GCCGAG 1 cut(s) 82
NsiI ATGCAT 1 cut(s) 206
PaeR7I CTCGAG 1 cut(s) 36
PctI GAATGC 1 cut(s) 204
PkrI GCNGC 2 cut(s) 9, 97
PleI GAGTC 1 cut(s) 202
PpsI GAGTC 1 cut(s) 202
PshBI ATTAAT 1 cut(s) 242
PspXI VCTCGAGB 1 cut(s) 36
PsrI GAACNNNNNNTAC 2 cut(s) 53, 85
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
SaqAI TTAA 1 cut(s) 242
SatI GCNGC 2 cut(s) 8, 96
Sau3AI GATC 1 cut(s) 18
SchI GAGTC 1 cut(s) 203
SetI ASST 4 cut(s) 29, 117, 164, 174
SfcI CTRYAG 1 cut(s) 22
Sfr274I CTCGAG 1 cut(s) 36
SlaI CTCGAG 1 cut(s) 36
SmlI CTYRAG 1 cut(s) 36
SmoI CTYRAG 1 cut(s) 36
Sse9I AATT 4 cut(s) 79, 145, 166, 243
SsiI CCGC 1 cut(s) 96
TaqI TCGA 2 cut(s) 37, 177
TasI AATT 4 cut(s) 79, 145, 166, 243
TatI WGTACW 1 cut(s) 60
TauI GCSGC 1 cut(s) 98
Tru1I TTAA 1 cut(s) 242
Tru9I TTAA 1 cut(s) 242
TscAI CASTG 1 cut(s) 196
TseI GCWGC 1 cut(s) 7
TspDTI ATGAA 1 cut(s) 197
TspRI CASTG 1 cut(s) 196
VspI ATTAAT 1 cut(s) 242
XapI RAATTY 1 cut(s) 79
XhoI CTCGAG 1 cut(s) 36
Zsp2I ATGCAT 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.