MD04G1021800.v1.1

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0020352
Physical Location & Seq
Reverse (-)
2567896 .. 2568558
663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1021800.v1.1.491

Sequence Viewer

Length: 270 bp
ATGAAAATTAGAAATGGAGGCCACTTTTGTGTATCAGAGAGCGAGTACATGATTCCTTTTGCTTCCATGAATCCAAGGGAAGCCGCCTTCATCTACAAAGATCTTGACCTGGGGATCAACCACAATGGCAAGGCAAGCTATTCTGAGGGAAGAGTCTATGGAACCAGGTACTTGAAGGATAATTTCGATCGATTGGTGCGTATTAAGACCAACATCGATCCGGGTAATACTTTTAGGAATGAACAAAGTATCCCAACTCTTCCATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

90

Amino Acids

10.24

Weight (kDa)

8.82

Isoelectric Point (pI)

29.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BBE PF08031 32 - 85 1.2e-14 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020352)

Species Orthologous Gene IDs
malus_domestica MD04G1021800.v1.1 MD05G1266500.v1.1 MD10G1244100.v1.1
rosa_chinensis RchiOBHm_Chr5g0017941
rosa_multiflora Rmu_sc0003834.1_g000006
rosa_rugosa Rorug05G0038900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 84
AclWI GGATC 2 cut(s) 122, 212
AfaI GTAC 2 cut(s) 47, 170
AgsI TTSAA 1 cut(s) 175
AjnI CCWGG 2 cut(s) 108, 164
AleI CACNNNNGTG 1 cut(s) 27
AloI GAACNNNNNNTCC 2 cut(s) 234, 266
AluBI AGCT 1 cut(s) 138
AluI AGCT 1 cut(s) 138
AlwI GGATC 2 cut(s) 122, 212
AoxI GGCC 1 cut(s) 19
AsuC2I CCSGG 1 cut(s) 222
BciT130I CCWGG 2 cut(s) 110, 166
BciVI GTATCC 1 cut(s) 260
BcnI CCSGG 1 cut(s) 222
BfaI CTAG 1 cut(s) 268
BfuI GTATCC 1 cut(s) 260
BglII AGATCT 1 cut(s) 100
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 3 cut(s) 110, 166, 222
BmiI GGNNCC 1 cut(s) 163
BmrFI CCNGG 3 cut(s) 110, 166, 222
BpuMI CCSGG 1 cut(s) 222
Bsa29I ATCGAT 2 cut(s) 190, 216
BsaJI CCNNGG 2 cut(s) 74, 109
BseBI CCWGG 2 cut(s) 110, 166
BseCI ATCGAT 2 cut(s) 190, 216
BseDI CCNNGG 2 cut(s) 74, 109
BseMII CTCAG 1 cut(s) 135
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 1 cut(s) 21
BshVI ATCGAT 2 cut(s) 190, 216
BsiEI CGRYCG 1 cut(s) 190
BsiSI CCGG 1 cut(s) 221
BsnI GGCC 1 cut(s) 21
Bsp143I GATC 4 cut(s) 100, 114, 187, 217
BspACI CCGC 1 cut(s) 84
BspANI GGCC 1 cut(s) 21
BspCNI CTCAG 1 cut(s) 136
BspDI ATCGAT 2 cut(s) 190, 216
BspLI GGNNCC 1 cut(s) 163
BspPI GGATC 2 cut(s) 122, 212
BssECI CCNNGG 2 cut(s) 74, 109
BssMI GATC 4 cut(s) 100, 114, 187, 217
BssT1I CCWWGG 1 cut(s) 74
Bst2UI CCWGG 2 cut(s) 110, 166
Bst6I CTCTTC 2 cut(s) 145, 264
BstC8I GCNNGC 1 cut(s) 136
BstDEI CTNAG 1 cut(s) 144
BstKTI GATC 4 cut(s) 103, 117, 190, 220
BstMBI GATC 4 cut(s) 100, 114, 187, 217
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 1 cut(s) 135
BstNI CCWGG 2 cut(s) 110, 166
BstSCI CCNGG 3 cut(s) 108, 164, 220
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
Bsu15I ATCGAT 2 cut(s) 190, 216
BsuI GTATCC 1 cut(s) 260
BsuRI GGCC 1 cut(s) 21
BsuTUI ATCGAT 2 cut(s) 190, 216
Cac8I GCNNGC 1 cut(s) 136
ClaI ATCGAT 2 cut(s) 190, 216
CsiI ACCWGGT 1 cut(s) 164
Csp6I GTAC 2 cut(s) 46, 169
CviAII CATG 2 cut(s) 49, 67
CviJI RGCY 3 cut(s) 21, 83, 138
CviKI_1 RGCY 3 cut(s) 21, 83, 138
CviQI GTAC 2 cut(s) 46, 169
DdeI CTNAG 1 cut(s) 144
DpnI GATC 4 cut(s) 102, 116, 189, 219
DpnII GATC 4 cut(s) 100, 114, 187, 217
Eam1104I CTCTTC 2 cut(s) 145, 264
EarI CTCTTC 2 cut(s) 145, 264
Eco130I CCWWGG 1 cut(s) 74
EcoRII CCWGG 2 cut(s) 108, 164
EcoT14I CCWWGG 1 cut(s) 74
ErhI CCWWGG 1 cut(s) 74
FaeI CATG 2 cut(s) 52, 70
FaiI YATR 3 cut(s) 50, 68, 159
FatI CATG 2 cut(s) 48, 66
Fnu4HI GCNGC 1 cut(s) 84
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 1 cut(s) 268
GluI GCNGC 1 cut(s) 84
HaeIII GGCC 1 cut(s) 21
HapII CCGG 1 cut(s) 221
Hin1II CATG 2 cut(s) 52, 70
HinfI GANTC 3 cut(s) 52, 70, 153
HpaII CCGG 1 cut(s) 221
Hpy188I TCNGA 2 cut(s) 37, 145
Hpy188III TCNNGA 1 cut(s) 104
HpyAV CCTTC 2 cut(s) 97, 169
HpyF10VI GCNNNNNNNGC 1 cut(s) 135
HpyF3I CTNAG 1 cut(s) 144
Hsp92II CATG 2 cut(s) 52, 70
Kzo9I GATC 4 cut(s) 100, 114, 187, 217
LpnPI CCDG 5 cut(s) 95, 122, 151, 178, 234
MabI ACCWGGT 1 cut(s) 164
MaeI CTAG 1 cut(s) 268
MalI GATC 4 cut(s) 102, 116, 189, 219
MboI GATC 4 cut(s) 100, 114, 187, 217
MboII GAAGA 2 cut(s) 162, 251
MflI RGATCY 1 cut(s) 100
MluCI AATT 2 cut(s) 6, 181
MlyI GAGTC 1 cut(s) 162
MnlI CCTC 2 cut(s) 11, 139
MseI TTAA 1 cut(s) 204
MslI CAYNNNNRTG 1 cut(s) 27
MspI CCGG 1 cut(s) 221
MspR9I CCNGG 3 cut(s) 110, 166, 222
MvaI CCWGG 2 cut(s) 110, 166
MwoI GCNNNNNNNGC 1 cut(s) 135
NciI CCSGG 1 cut(s) 222
NdeII GATC 4 cut(s) 100, 114, 187, 217
NlaIII CATG 2 cut(s) 52, 70
NlaIV GGNNCC 1 cut(s) 163
OliI CACNNNNGTG 1 cut(s) 27
PcsI WCGNNNNNNNCGW 1 cut(s) 196
PfeI GAWTC 2 cut(s) 52, 70
PkrI GCNGC 1 cut(s) 85
Ple19I CGATCG 1 cut(s) 190
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
Psp6I CCWGG 2 cut(s) 108, 164
PspGI CCWGG 2 cut(s) 108, 164
PspN4I GGNNCC 1 cut(s) 163
PsuI RGATCY 1 cut(s) 100
PvuI CGATCG 1 cut(s) 190
RsaI GTAC 2 cut(s) 47, 170
RsaNI GTAC 2 cut(s) 46, 169
RseI CAYNNNNRTG 1 cut(s) 27
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 4 cut(s) 100, 114, 187, 217
SchI GAGTC 1 cut(s) 162
ScrFI CCNGG 3 cut(s) 110, 166, 222
SetI ASST 3 cut(s) 111, 140, 170
SexAI ACCWGGT 1 cut(s) 164
SmiMI CAYNNNNRTG 1 cut(s) 27
Sse9I AATT 2 cut(s) 6, 181
SsiI CCGC 1 cut(s) 84
SspMI CTAG 1 cut(s) 268
StyD4I CCNGG 3 cut(s) 108, 164, 220
StyI CCWWGG 1 cut(s) 74
TaqI TCGA 3 cut(s) 186, 190, 216
TasI AATT 2 cut(s) 6, 181
TatI WGTACW 1 cut(s) 45
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 2 cut(s) 52, 70
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TspDTI ATGAA 4 cut(s) 17, 79, 83, 255
XspI CTAG 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.