MD04G1026500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014714
Physical Location & Seq
Forward (+)
3061766 .. 3064823
3058 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1026500.v1.1.491

Sequence Viewer

Length: 909 bp
ATGCTTGAATCTCAGCTGAAAAACCCAGCTCGGGCTGGGACTGGGGTTTCACAATCTCTGCAGAAACAAGTAGCTCCAAATGATAACCCCGGTCAACCAGAATCGCCCTTCTCCATAAGACAGTATGTTCTTGATTGTCGCCAGAAAGATGTATTTTCTAACTGGCCATTTCCAGAGAAATACTTGCAAATTTGTCTCGACCATGGTATTAGTGATGCGTTGCCGCCCTTTGAATCCCATACTTCAGCTATCAAATCGATTAGGGGAGCGGTAGGTTTGAATTGTACTCAACAAGATGATAACAATGCTGTTGACTTTGAAAGCGGAGTACCAGATATCATTGAGGAGACTGAAGATGGGAGTAAACTATACTCTGACAATGAGGAAGCAATATCAAAACTTTCAAATCAGCACTGCCATTTCTCTCCTTCAAATAGTAGTCGTGAGCGTGAAGAAAACAAGGCGGACTTTAGTTCCCACATATCATCCAATAGCATAGTTTCAACTGATCAACCTTCAACTACAATACCAAGTTCACACCTTAATGTTGTTGAGTGCAATGAAACTTTGACTTCTTCTAGGAAGCCAAGGCATATGAAAAAACGACACAGGGGTAAGCATAAGAAGAGGTCGATGGGGAATATCTTAGATGTAGCCAAGCCTTGTACACCGGAGGATCTCCTTAGAATTAAAAGATTGTGCTGTGTTTCAAGCGTACCTCTTGTGAAGGGGGGTGAAGCAATTGAGAATTTGATCGATGCTAAGCAAGATTGCAACTCTGATCTAACCAAGGATAACTCGAGTGAAAAGCTTGAGAGAGATGGTAGTGAAGCAGCGAATCTCAGCATGCAGTCCAGCAAAAAATTTGTAGTGAAGTTCACGTTTAGCGGTTGCAAATCGAATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

303

Amino Acids

33.38

Weight (kDa)

6.32

Isoelectric Point (pI)

60.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014714)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g12430 FvH4_4g12430
malus_domestica MD04G1026500.v1.1
prunus_persica Prupe.1G176100_v2.0.a1 Prupe.1G176100_v2.0.a1
pyrus_communis pycom04g02260
rosa_chinensis RchiOBHm_Chr4g0410661
rosa_laevigata RLG00000008408
rosa_multiflora Rmu_ssc0000072.1_g000017
rosa_roxburghii Rroxscaffold_5G00355440
rosa_rugosa Rorug04G0111600 Rorug04G0111700
rosa_samantha Rh4AG162200 Rh4BG161200 Rh4CG175200 Rh4DG158000
rosa_wichuraiana Rw4G013530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 269
AciI CCGC 5 cut(s) 224, 269, 324, 464, 888
AclWI GGATC 1 cut(s) 684
AcoI YGGCCR 1 cut(s) 164
AcsI RAATTY 3 cut(s) 189, 748, 863
AcuI CTGAAG 2 cut(s) 228, 372
AfaI GTAC 4 cut(s) 286, 330, 667, 717
AfiI CCNNNNNNNGG 1 cut(s) 31
AgsI TTSAA 9 cut(s) 8, 233, 280, 320, 405, 432, 504, 519, 711
AluBI AGCT 5 cut(s) 16, 29, 74, 248, 811
AluI AGCT 5 cut(s) 16, 29, 74, 248, 811
Alw26I GTCTC 2 cut(s) 200, 341
AlwI GGATC 1 cut(s) 684
Ama87I CYCGRG 2 cut(s) 30, 799
AoxI GGCC 1 cut(s) 164
ApeKI GCWGC 1 cut(s) 833
ApoI RAATTY 3 cut(s) 189, 748, 863
ArsI GACNNNNNNTTYG 1 cut(s) 888
AsuC2I CCSGG 1 cut(s) 90
AsuHPI GGTGA 1 cut(s) 746
AvaI CYCGRG 2 cut(s) 30, 799
BalI TGGCCA 1 cut(s) 166
BbvI GCAGC 1 cut(s) 845
BccI CCATC 3 cut(s) 350, 628, 815
BclI TGATCA 1 cut(s) 508
BcnI CCSGG 1 cut(s) 90
BcoDI GTCTC 2 cut(s) 200, 341
BfaI CTAG 1 cut(s) 579
BfmI CTRYAG 1 cut(s) 59
BisI GCNGC 2 cut(s) 224, 834
BlpI GCTNAGC 1 cut(s) 762
BlsI GCNGC 2 cut(s) 225, 835
Bme1390I CCNGG 1 cut(s) 90
BmeT110I CYCGRG 2 cut(s) 30, 799
BmrFI CCNGG 1 cut(s) 90
BmrI ACTGGG 1 cut(s) 51
BmsI GCATC 2 cut(s) 205, 748
BmuI ACTGGG 1 cut(s) 51
Bpu1102I GCTNAGC 1 cut(s) 762
BpuEI CTTGAG 1 cut(s) 833
BpuMI CCSGG 1 cut(s) 90
Bsa29I ATCGAT 2 cut(s) 257, 756
BsaJI CCNNGG 4 cut(s) 88, 202, 587, 789
BsaWI WCCGGW 1 cut(s) 670
Bsc4I CCNNNNNNNGG 1 cut(s) 31
Bse1I ACTGG 2 cut(s) 46, 167
Bse3DI GCAATG 1 cut(s) 565
BseCI ATCGAT 2 cut(s) 257, 756
BseDI CCNNGG 4 cut(s) 88, 202, 587, 789
BseGI GGATG 1 cut(s) 485
BseLI CCNNNNNNNGG 1 cut(s) 31
BseMI GCAATG 1 cut(s) 565
BseMII CTCAG 2 cut(s) 26, 856
BseNI ACTGG 2 cut(s) 46, 167
BseRI GAGGAG 1 cut(s) 359
BseXI GCAGC 1 cut(s) 845
BseYI CCCAGC 2 cut(s) 25, 35
BshFI GGCC 1 cut(s) 166
BshVI ATCGAT 2 cut(s) 257, 756
BsiHKCI CYCGRG 2 cut(s) 30, 799
BsiSI CCGG 2 cut(s) 90, 671
BslFI GGGAC 1 cut(s) 52
BslI CCNNNNNNNGG 1 cut(s) 31
BsmAI GTCTC 2 cut(s) 200, 341
BsmFI GGGAC 1 cut(s) 52
BsnI GGCC 1 cut(s) 166
BsoBI CYCGRG 2 cut(s) 30, 799
Bsp1407I TGTACA 1 cut(s) 665
Bsp143I GATC 4 cut(s) 508, 676, 753, 781
Bsp1720I GCTNAGC 1 cut(s) 762
Bsp19I CCATGG 1 cut(s) 202
BspACI CCGC 5 cut(s) 224, 269, 324, 464, 888
BspANI GGCC 1 cut(s) 166
BspCNI CTCAG 2 cut(s) 25, 855
BspDI ATCGAT 2 cut(s) 257, 756
BspMAI CTGCAG 1 cut(s) 63
BspPI GGATC 1 cut(s) 684
BsrBI CCGCTC 1 cut(s) 269
BsrDI GCAATG 1 cut(s) 565
BsrGI TGTACA 1 cut(s) 665
BsrI ACTGG 2 cut(s) 46, 167
BssECI CCNNGG 4 cut(s) 88, 202, 587, 789
BssMI GATC 4 cut(s) 508, 676, 753, 781
BssT1I CCWWGG 3 cut(s) 202, 587, 789
Bst4CI ACNGT 1 cut(s) 123
Bst6I CTCTTC 1 cut(s) 620
BstAUI TGTACA 1 cut(s) 665
BstC8I GCNNGC 1 cut(s) 848
BstDEI CTNAG 5 cut(s) 12, 646, 683, 762, 842
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 1 cut(s) 485
BstKTI GATC 4 cut(s) 511, 679, 756, 784
BstMAI GTCTC 2 cut(s) 200, 341
BstMBI GATC 4 cut(s) 508, 676, 753, 781
BstNSI RCATGY 1 cut(s) 850
BstSCI CCNGG 1 cut(s) 88
BstSFI CTRYAG 1 cut(s) 59
BstV1I GCAGC 1 cut(s) 845
BstX2I RGATCY 1 cut(s) 676
BstYI RGATCY 1 cut(s) 676
Bsu15I ATCGAT 2 cut(s) 257, 756
BsuRI GGCC 1 cut(s) 166
BsuTUI ATCGAT 2 cut(s) 257, 756
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 1 cut(s) 485
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 1 cut(s) 412
Cac8I GCNNGC 1 cut(s) 848
ClaI ATCGAT 2 cut(s) 257, 756
Csp6I GTAC 4 cut(s) 285, 329, 666, 716
CviAII CATG 2 cut(s) 203, 847
CviQI GTAC 4 cut(s) 285, 329, 666, 716
DdeI CTNAG 5 cut(s) 12, 646, 683, 762, 842
DpnI GATC 4 cut(s) 510, 678, 755, 783
DpnII GATC 4 cut(s) 508, 676, 753, 781
EaeI YGGCCR 1 cut(s) 164
Eam1104I CTCTTC 1 cut(s) 620
EarI CTCTTC 1 cut(s) 620
EciI GGCGGA 1 cut(s) 479
Eco130I CCWWGG 3 cut(s) 202, 587, 789
Eco32I GATATC 1 cut(s) 337
Eco57I CTGAAG 2 cut(s) 228, 372
Eco88I CYCGRG 2 cut(s) 30, 799
EcoRV GATATC 1 cut(s) 337
EcoT14I CCWWGG 3 cut(s) 202, 587, 789
ErhI CCWWGG 3 cut(s) 202, 587, 789
FaeI CATG 2 cut(s) 206, 850
FalI AAGNNNNNCTT 2 cut(s) 452, 484
FaqI GGGAC 1 cut(s) 52
FatI CATG 2 cut(s) 202, 846
FauNDI CATATG 1 cut(s) 594
FbaI TGATCA 1 cut(s) 508
Fnu4HI GCNGC 2 cut(s) 224, 834
FokI GGATG 1 cut(s) 472
Fsp4HI GCNGC 2 cut(s) 224, 834
FspBI CTAG 1 cut(s) 579
GluI GCNGC 2 cut(s) 224, 834
GsaI CCCAGC 2 cut(s) 29, 39
HaeIII GGCC 1 cut(s) 166
HapII CCGG 2 cut(s) 90, 671
Hin1II CATG 2 cut(s) 206, 850
HincII GTYRAC 2 cut(s) 95, 313
HindII GTYRAC 2 cut(s) 95, 313
HindIII AAGCTT 1 cut(s) 809
HinfI GANTC 4 cut(s) 8, 101, 233, 838
HpaII CCGG 2 cut(s) 90, 671
HphI GGTGA 1 cut(s) 746
Hpy166II GTNNAC 6 cut(s) 95, 313, 365, 536, 668, 879
Hpy188I TCNGA 3 cut(s) 376, 781, 908
Hpy188III TCNNGA 4 cut(s) 131, 173, 197, 443
Hpy8I GTNNAC 6 cut(s) 95, 313, 365, 536, 668, 879
HpyAV CCTTC 4 cut(s) 118, 438, 525, 721
HpyCH4III ACNGT 1 cut(s) 123
HpyCH4IV ACGT 1 cut(s) 881
HpyCH4V TGCA 6 cut(s) 61, 187, 558, 774, 850, 894
HpyF3I CTNAG 5 cut(s) 12, 646, 683, 762, 842
HpySE526I ACGT 1 cut(s) 881
Hsp92II CATG 2 cut(s) 206, 850
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 4 cut(s) 508, 676, 753, 781
LmnI GCTCC 2 cut(s) 79, 266
Lsp1109I GCAGC 1 cut(s) 845
LweI GCATC 2 cut(s) 205, 748
MaeI CTAG 1 cut(s) 579
MaeII ACGT 1 cut(s) 881
MalI GATC 4 cut(s) 510, 678, 755, 783
MbiI CCGCTC 1 cut(s) 269
MboI GATC 4 cut(s) 508, 676, 753, 781
MboII GAAGA 4 cut(s) 365, 464, 567, 637
MfeI CAATTG 1 cut(s) 741
MflI RGATCY 1 cut(s) 676
MlsI TGGCCA 1 cut(s) 166
MluCI AATT 6 cut(s) 189, 280, 687, 741, 748, 863
MluNI TGGCCA 1 cut(s) 166
MnlI CCTC 5 cut(s) 337, 376, 621, 667, 729
Mox20I TGGCCA 1 cut(s) 166
MscI TGGCCA 1 cut(s) 166
MseI TTAA 2 cut(s) 543, 690
MslI CAYNNNNRTG 1 cut(s) 543
Msp20I TGGCCA 1 cut(s) 166
MspA1I CMGCKG 1 cut(s) 16
MspI CCGG 2 cut(s) 90, 671
MspR9I CCNGG 1 cut(s) 90
MunI CAATTG 1 cut(s) 741
NciI CCSGG 1 cut(s) 90
NcoI CCATGG 1 cut(s) 202
NdeI CATATG 1 cut(s) 594
NdeII GATC 4 cut(s) 508, 676, 753, 781
NlaIII CATG 2 cut(s) 206, 850
NspI RCATGY 1 cut(s) 850
PaeI GCATGC 1 cut(s) 850
PaeR7I CTCGAG 1 cut(s) 799
PfeI GAWTC 4 cut(s) 8, 101, 233, 838
PkrI GCNGC 2 cut(s) 225, 835
PspFI CCCAGC 2 cut(s) 25, 35
PspXI VCTCGAGB 1 cut(s) 799
PstI CTGCAG 1 cut(s) 63
PsuI RGATCY 1 cut(s) 676
PvuII CAGCTG 1 cut(s) 16
RsaI GTAC 4 cut(s) 286, 330, 667, 717
RsaNI GTAC 4 cut(s) 285, 329, 666, 716
RseI CAYNNNNRTG 1 cut(s) 543
SaqAI TTAA 2 cut(s) 543, 690
SatI GCNGC 2 cut(s) 224, 834
Sau3AI GATC 4 cut(s) 508, 676, 753, 781
ScrFI CCNGG 1 cut(s) 90
SfaNI GCATC 2 cut(s) 205, 748
SfcI CTRYAG 1 cut(s) 59
Sfr274I CTCGAG 1 cut(s) 799
SlaI CTCGAG 1 cut(s) 799
SmiMI CAYNNNNRTG 1 cut(s) 543
SmlI CTYRAG 2 cut(s) 799, 812
SmoI CTYRAG 2 cut(s) 799, 812
SphI GCATGC 1 cut(s) 850
Sse9I AATT 6 cut(s) 189, 280, 687, 741, 748, 863
SsiI CCGC 5 cut(s) 224, 269, 324, 464, 888
SspMI CTAG 1 cut(s) 579
StyD4I CCNGG 1 cut(s) 88
StyI CCWWGG 3 cut(s) 202, 587, 789
TaaI ACNGT 1 cut(s) 123
TaiI ACGT 1 cut(s) 884
TaqI TCGA 6 cut(s) 198, 257, 632, 756, 800, 899
TasI AATT 6 cut(s) 189, 280, 687, 741, 748, 863
TatI WGTACW 2 cut(s) 284, 665
TauI GCSGC 1 cut(s) 226
TfiI GAWTC 4 cut(s) 8, 101, 233, 838
Tru1I TTAA 2 cut(s) 543, 690
Tru9I TTAA 2 cut(s) 543, 690
TscAI CASTG 1 cut(s) 419
TseI GCWGC 1 cut(s) 833
TspDTI ATGAA 2 cut(s) 576, 611
TspRI CASTG 1 cut(s) 419
XapI RAATTY 3 cut(s) 189, 748, 863
XceI RCATGY 1 cut(s) 850
XhoI CTCGAG 1 cut(s) 799
XspI CTAG 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.