MD04G1032400.v1.1

Vesicle transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008997
Physical Location & Seq
Forward (+)
3651460 .. 3664497
13038 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1032400.v1.1.491

Sequence Viewer

Length: 528 bp
ATGGCTTATGAATTGAGTGAACAGAAAAAGGTTGGGTTGGGTCTCCTTGGCTTTGGCATCCTTTTCACATTTCTGGGTGTGATGCTTTTCTTCGACAGAGGCTTGCTTGCTCTAGGGAATATATTTTGGTTGACTGGGGTGGCCCTTTTACTTGGTTGGCGTTCTACATGGAATCTCTTCACTAGCAAAGCAAACTTAAAGGGCTCTGTATCTTTTCTTCTTGGTCTCTTTTTCCTTTTCGTTCGGTGGCCAATAGTTGGTATAATCTTGGAAATATATGGCTGCCTTAACTTGTTCGGTCGTTTTTGGCCATCTGTGAAGGCATTCCTCTATGAAGTTCCCATGTTGGGATGGATCATAAGATTCTTGATCAACTTCAGGGCAGTGATGCTTGACGTCTACTTATATAACAGCTCGTGTCTCACAGGGTTTCCTGATTCTTCAGAAGCCGAACAAGGTCTAAGCAACTGGCTTCTCATGGGTTTAAGATTCCATCATCAGATGAGTGAAAAAGAAGAAAGAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Got1

Protein Analysis

176

Amino Acids

20.09

Weight (kDa)

7.78

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Got1 PF04178 15 - 114 4.5e-10 Got1/Sft2-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 399
AccB7I CCANNNNNTGG 1 cut(s) 257
AccI GTMKAC 1 cut(s) 399
AclWI GGATC 1 cut(s) 362
AcoI YGGCCR 2 cut(s) 248, 308
AcuI CTGAAG 2 cut(s) 361, 426
AcyI GRCGYC 1 cut(s) 396
AfiI CCNNNNNNNGG 2 cut(s) 257, 347
AluBI AGCT 1 cut(s) 414
AluI AGCT 1 cut(s) 414
Alw26I GTCTC 4 cut(s) 47, 230, 425, 516
AlwI GGATC 1 cut(s) 362
AoxI GGCC 3 cut(s) 141, 248, 308
ApeKI GCWGC 1 cut(s) 282
Asp700I GAANNNNTTC 2 cut(s) 176, 323
AspS9I GGNCC 1 cut(s) 142
BalI TGGCCA 2 cut(s) 250, 310
BanII GRGCYC 1 cut(s) 206
BarI GAAGNNNNNNTAC 2 cut(s) 201, 233
BauI CACGAG 1 cut(s) 415
BbvI GCAGC 1 cut(s) 269
BccI CCATC 3 cut(s) 319, 345, 501
BclI TGATCA 1 cut(s) 369
BcoDI GTCTC 4 cut(s) 47, 230, 425, 516
BfaI CTAG 3 cut(s) 113, 183, 526
BisI GCNGC 1 cut(s) 283
BlsI GCNGC 1 cut(s) 284
BmgT120I GGNCC 1 cut(s) 142
BmrI ACTGGG 1 cut(s) 144
BmsI GCATC 3 cut(s) 66, 72, 378
BmuI ACTGGG 1 cut(s) 144
BsaHI GRCGYC 1 cut(s) 396
BsaI GGTCTC 2 cut(s) 47, 230
BsaJI CCNNGG 1 cut(s) 46
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 347
Bse1I ACTGG 2 cut(s) 139, 473
BseDI CCNNGG 1 cut(s) 46
BseGI GGATG 2 cut(s) 57, 356
BseLI CCNNNNNNNGG 2 cut(s) 257, 347
BseNI ACTGG 2 cut(s) 139, 473
BseXI GCAGC 1 cut(s) 269
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 3 cut(s) 143, 250, 310
BsiEI CGRYCG 1 cut(s) 301
BslI CCNNNNNNNGG 2 cut(s) 257, 347
BsmAI GTCTC 4 cut(s) 47, 230, 425, 516
BsmI GAATGC 1 cut(s) 323
BsnI GGCC 3 cut(s) 143, 250, 310
Bso31I GGTCTC 2 cut(s) 47, 230
Bsp1286I GDGCHC 1 cut(s) 206
Bsp143I GATC 2 cut(s) 354, 369
BspANI GGCC 3 cut(s) 143, 250, 310
BspPI GGATC 1 cut(s) 362
BspTNI GGTCTC 2 cut(s) 47, 230
BsrI ACTGG 2 cut(s) 139, 473
BssECI CCNNGG 1 cut(s) 46
BssMI GATC 2 cut(s) 354, 369
BssNI GRCGYC 1 cut(s) 396
BssSI CACGAG 1 cut(s) 415
BssT1I CCWWGG 1 cut(s) 46
Bst2BI CACGAG 1 cut(s) 415
Bst6I CTCTTC 1 cut(s) 182
BstACI GRCGYC 1 cut(s) 396
BstC8I GCNNGC 2 cut(s) 104, 108
BstDEI CTNAG 1 cut(s) 461
BstF5I GGATG 2 cut(s) 57, 356
BstKTI GATC 2 cut(s) 357, 372
BstMAI GTCTC 4 cut(s) 47, 230, 425, 516
BstMBI GATC 2 cut(s) 354, 369
BstMCI CGRYCG 1 cut(s) 301
BstV1I GCAGC 1 cut(s) 269
BsuRI GGCC 3 cut(s) 143, 250, 310
BtsCI GGATG 2 cut(s) 57, 356
BtsI GCAGTG 1 cut(s) 390
BtsIMutI CAGTG 1 cut(s) 390
Cac8I GCNNGC 2 cut(s) 104, 108
Cfr13I GGNCC 1 cut(s) 142
CviAII CATG 3 cut(s) 168, 343, 478
DdeI CTNAG 1 cut(s) 461
DpnI GATC 2 cut(s) 356, 371
DpnII GATC 2 cut(s) 354, 369
EaeI YGGCCR 2 cut(s) 248, 308
Eam1104I CTCTTC 1 cut(s) 182
EarI CTCTTC 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 46
Eco24I GRGCYC 1 cut(s) 206
Eco31I GGTCTC 2 cut(s) 47, 230
Eco57I CTGAAG 2 cut(s) 361, 426
EcoT14I CCWWGG 1 cut(s) 46
EcoT38I GRGCYC 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 46
FaeI CATG 3 cut(s) 171, 346, 481
FatI CATG 3 cut(s) 167, 342, 477
FbaI TGATCA 1 cut(s) 369
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 1 cut(s) 283
FokI GGATG 2 cut(s) 44, 363
FriOI GRGCYC 1 cut(s) 206
Fsp4HI GCNGC 1 cut(s) 283
FspBI CTAG 3 cut(s) 113, 183, 526
GluI GCNGC 1 cut(s) 283
HaeIII GGCC 3 cut(s) 143, 250, 310
Hin1I GRCGYC 1 cut(s) 396
Hin1II CATG 3 cut(s) 171, 346, 481
HincII GTYRAC 1 cut(s) 132
HindII GTYRAC 1 cut(s) 132
HinfI GANTC 4 cut(s) 172, 363, 437, 489
Hpy166II GTNNAC 3 cut(s) 20, 132, 400
Hpy188I TCNGA 2 cut(s) 445, 501
Hpy188III TCNNGA 2 cut(s) 367, 434
Hpy8I GTNNAC 3 cut(s) 20, 132, 400
HpyAV CCTTC 1 cut(s) 313
HpyCH4IV ACGT 1 cut(s) 396
HpyF3I CTNAG 1 cut(s) 461
HpySE526I ACGT 1 cut(s) 396
Hsp92I GRCGYC 1 cut(s) 396
Hsp92II CATG 3 cut(s) 171, 346, 481
Ksp22I TGATCA 1 cut(s) 369
Kzo9I GATC 2 cut(s) 354, 369
LpnPI CCDG 6 cut(s) 59, 120, 364, 411, 447, 454
Lsp1109I GCAGC 1 cut(s) 269
LweI GCATC 3 cut(s) 66, 72, 378
MaeI CTAG 3 cut(s) 113, 183, 526
MaeII ACGT 1 cut(s) 396
MalI GATC 2 cut(s) 356, 371
MboI GATC 2 cut(s) 354, 369
MboII GAAGA 5 cut(s) 82, 169, 209, 432, 527
MhlI GDGCHC 1 cut(s) 206
MlsI TGGCCA 2 cut(s) 250, 310
MluCI AATT 1 cut(s) 11
MluNI TGGCCA 2 cut(s) 250, 310
MnlI CCTC 2 cut(s) 92, 338
Mox20I TGGCCA 2 cut(s) 250, 310
MroXI GAANNNNTTC 2 cut(s) 176, 323
MscI TGGCCA 2 cut(s) 250, 310
MseI TTAA 3 cut(s) 197, 288, 485
Msp20I TGGCCA 2 cut(s) 250, 310
Mva1269I GAATGC 1 cut(s) 323
NdeII GATC 2 cut(s) 354, 369
NlaIII CATG 3 cut(s) 171, 346, 481
PctI GAATGC 1 cut(s) 323
PdmI GAANNNNTTC 2 cut(s) 176, 323
PfeI GAWTC 4 cut(s) 172, 363, 437, 489
PflMI CCANNNNNTGG 1 cut(s) 257
PkrI GCNGC 1 cut(s) 284
PspPI GGNCC 1 cut(s) 142
SaqAI TTAA 3 cut(s) 197, 288, 485
SatI GCNGC 1 cut(s) 283
Sau3AI GATC 2 cut(s) 354, 369
Sau96I GGNCC 1 cut(s) 142
SduI GDGCHC 1 cut(s) 206
SetI ASST 4 cut(s) 33, 399, 416, 460
SfaNI GCATC 3 cut(s) 66, 72, 378
Sse9I AATT 1 cut(s) 11
SspMI CTAG 3 cut(s) 113, 183, 526
StyI CCWWGG 1 cut(s) 46
TaiI ACGT 1 cut(s) 399
TaqI TCGA 1 cut(s) 93
TaqII GACCGA 1 cut(s) 287
TasI AATT 1 cut(s) 11
TfiI GAWTC 4 cut(s) 172, 363, 437, 489
Tru1I TTAA 3 cut(s) 197, 288, 485
Tru9I TTAA 3 cut(s) 197, 288, 485
TscAI CASTG 1 cut(s) 390
TseI GCWGC 1 cut(s) 282
TspDTI ATGAA 2 cut(s) 24, 348
TspRI CASTG 1 cut(s) 390
Van91I CCANNNNNTGG 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 399
XmnI GAANNNNTTC 2 cut(s) 176, 323
XspI CTAG 3 cut(s) 113, 183, 526
ZraI GACGTC 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.