MD04G1040200.v1.1

Ribosomal L18p L5e family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0019643
Physical Location & Seq
Forward (+)
4707709 .. 4708086
378 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1040200.v1.1.491

Sequence Viewer

Length: 378 bp
ATGTCCGGAAACCACCATGTCCTCCGCCTGGTGCTCTCATGCCGCAAAATCACAGCACAGGTGACGAGCCCCAGCAGCTCCTCAATCATAGCCATGGCCTCCTCCTCCGAGCAAGAGTTCGTCGCCCGCTACCGATCCAATCTCAACCGCTTCCCTCGCTCCCAAACCTTCTGGGACGCCAAGGTCGCCTCTCGCGTCGGCGAGAAGCTCGCCCTCCGTCTCCGGGATGTCGGCGTCACCGGCGTGGAGATCGACCTCCGCGAAGAGCTGGCCAGGCCTGTCCACCACCGCATCAGGGTGCTGCCGCTCTTCGATTCCGTTAAACGAGCCGGCGTTGTAGTCGATGGCGTTGACGAGCTTGGCCTTGGTGGGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

126

Amino Acids

13.78

Weight (kDa)

10.13

Isoelectric Point (pI)

55.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019643)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43310
fragaria_vesca FvH4_4g10741
malus_domestica MD04G1040200.v1.1 MD07G1010900.v1.1 MD07G1093600.v1.1 MD08G1180100.v1.1
prunus_persica Prupe.1G192700_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 182
AccBSI CCGCTC 1 cut(s) 307
AccII CGCG 2 cut(s) 195, 261
AccIII TCCGGA 1 cut(s) 5
AciI CCGC 7 cut(s) 25, 43, 127, 148, 259, 289, 305
AclWI GGATC 1 cut(s) 129
AcoI YGGCCR 1 cut(s) 270
AcyI GRCGYC 2 cut(s) 177, 234
AfiI CCNNNNNNNGG 3 cut(s) 28, 223, 295
AjnI CCWGG 2 cut(s) 27, 272
AleI CACNNNNGTG 1 cut(s) 242
AluBI AGCT 4 cut(s) 78, 208, 268, 358
AluI AGCT 4 cut(s) 78, 208, 268, 358
Alw21I GWGCWC 1 cut(s) 36
Alw26I GTCTC 1 cut(s) 224
AlwI GGATC 1 cut(s) 129
Aor13HI TCCGGA 1 cut(s) 5
AoxI GGCC 5 cut(s) 96, 270, 275, 361, 371
ApaI GGGCCC 1 cut(s) 375
ApeKI GCWGC 2 cut(s) 75, 301
AspS9I GGNCC 2 cut(s) 371, 372
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 2 cut(s) 73, 229
BaeGI GKGCMC 1 cut(s) 375
BalI TGGCCA 1 cut(s) 272
BanII GRGCYC 2 cut(s) 71, 375
Bbv12I GWGCWC 1 cut(s) 36
BbvI GCAGC 2 cut(s) 87, 288
BccI CCATC 1 cut(s) 338
BciT130I CCWGG 2 cut(s) 29, 274
BcnI CCSGG 1 cut(s) 224
BcoDI GTCTC 1 cut(s) 224
BfaI CTAG 1 cut(s) 376
BisI GCNGC 4 cut(s) 43, 76, 302, 305
BlsI GCNGC 4 cut(s) 44, 77, 303, 306
Bme1390I CCNGG 3 cut(s) 29, 224, 274
BmgT120I GGNCC 2 cut(s) 371, 372
BmiI GGNNCC 1 cut(s) 373
BmrFI CCNGG 3 cut(s) 29, 224, 274
BmsI GCATC 1 cut(s) 300
BpuMI CCSGG 1 cut(s) 224
BsaHI GRCGYC 2 cut(s) 177, 234
BsaJI CCNNGG 3 cut(s) 93, 180, 364
BsaWI WCCGGW 1 cut(s) 5
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 223, 295
Bse118I RCCGGY 2 cut(s) 239, 329
BseAI TCCGGA 1 cut(s) 5
BseBI CCWGG 2 cut(s) 29, 274
BseDI CCNNGG 3 cut(s) 93, 180, 364
BseGI GGATG 1 cut(s) 232
BseLI CCNNNNNNNGG 3 cut(s) 28, 223, 295
BseRI GAGGAG 3 cut(s) 70, 91, 94
BseSI GKGCMC 1 cut(s) 375
BseXI GCAGC 2 cut(s) 87, 288
BseYI CCCAGC 1 cut(s) 71
Bsh1236I CGCG 2 cut(s) 195, 261
BshFI GGCC 5 cut(s) 98, 272, 277, 363, 373
BsiHKAI GWGCWC 1 cut(s) 36
BsiSI CCGG 4 cut(s) 6, 223, 240, 330
BslFI GGGAC 1 cut(s) 188
BslI CCNNNNNNNGG 3 cut(s) 28, 223, 295
BsmAI GTCTC 1 cut(s) 224
BsmBI CGTCTC 1 cut(s) 224
BsmFI GGGAC 1 cut(s) 188
BsnI GGCC 5 cut(s) 98, 272, 277, 363, 373
Bsp120I GGGCCC 1 cut(s) 371
Bsp1286I GDGCHC 3 cut(s) 36, 71, 375
Bsp13I TCCGGA 1 cut(s) 5
Bsp143I GATC 2 cut(s) 134, 249
Bsp19I CCATGG 1 cut(s) 93
BspACI CCGC 7 cut(s) 25, 43, 127, 148, 259, 289, 305
BspANI GGCC 5 cut(s) 98, 272, 277, 363, 373
BspEI TCCGGA 1 cut(s) 5
BspFNI CGCG 2 cut(s) 195, 261
BspLI GGNNCC 1 cut(s) 373
BspPI GGATC 1 cut(s) 129
BspQI GCTCTTC 2 cut(s) 258, 314
BsrBI CCGCTC 1 cut(s) 307
BsrFI RCCGGY 2 cut(s) 239, 329
BssAI RCCGGY 2 cut(s) 239, 329
BssECI CCNNGG 3 cut(s) 93, 180, 364
BssMI GATC 2 cut(s) 134, 249
BssNI GRCGYC 2 cut(s) 177, 234
BssT1I CCWWGG 3 cut(s) 93, 180, 364
Bst2UI CCWGG 2 cut(s) 29, 274
Bst6I CTCTTC 2 cut(s) 258, 314
BstACI GRCGYC 2 cut(s) 177, 234
BstC8I GCNNGC 4 cut(s) 127, 210, 270, 331
BstDSI CCRYGG 1 cut(s) 93
BstF5I GGATG 1 cut(s) 232
BstFNI CGCG 2 cut(s) 195, 261
BstKTI GATC 2 cut(s) 137, 252
BstMAI GTCTC 1 cut(s) 224
BstMBI GATC 2 cut(s) 134, 249
BstMWI GCNNNNNNNGC 5 cut(s) 75, 156, 185, 240, 274
BstNI CCWGG 2 cut(s) 29, 274
BstSCI CCNGG 3 cut(s) 27, 222, 272
BstSLI GKGCMC 1 cut(s) 375
BstUI CGCG 2 cut(s) 195, 261
BstV1I GCAGC 2 cut(s) 87, 288
BsuRI GGCC 5 cut(s) 98, 272, 277, 363, 373
BtgI CCRYGG 1 cut(s) 93
BtsCI GGATG 1 cut(s) 232
Cac8I GCNNGC 4 cut(s) 127, 210, 270, 331
Cfr10I RCCGGY 2 cut(s) 239, 329
Cfr13I GGNCC 2 cut(s) 371, 372
CseI GACGC 3 cut(s) 184, 185, 223
CviAII CATG 3 cut(s) 17, 39, 94
DpnI GATC 2 cut(s) 136, 251
DpnII GATC 2 cut(s) 134, 249
DrdI GACNNNNNNGTC 1 cut(s) 182
DseDI GACNNNNNNGTC 1 cut(s) 182
EaeI YGGCCR 1 cut(s) 270
Eam1104I CTCTTC 2 cut(s) 258, 314
EarI CTCTTC 2 cut(s) 258, 314
EciI GGCGGA 1 cut(s) 14
Eco130I CCWWGG 3 cut(s) 93, 180, 364
Eco147I AGGCCT 1 cut(s) 277
Eco24I GRGCYC 2 cut(s) 71, 375
EcoO109I RGGNCCY 1 cut(s) 372
EcoRII CCWGG 2 cut(s) 27, 272
EcoT14I CCWWGG 3 cut(s) 93, 180, 364
EcoT38I GRGCYC 2 cut(s) 71, 375
ErhI CCWWGG 3 cut(s) 93, 180, 364
Esp3I CGTCTC 1 cut(s) 224
FaeI CATG 3 cut(s) 20, 42, 97
FaiI YATR 4 cut(s) 18, 40, 89, 95
FaqI GGGAC 1 cut(s) 188
FatI CATG 3 cut(s) 16, 38, 93
FauI CCCGC 1 cut(s) 134
Fnu4HI GCNGC 4 cut(s) 43, 76, 302, 305
FokI GGATG 1 cut(s) 239
FriOI GRGCYC 2 cut(s) 71, 375
Fsp4HI GCNGC 4 cut(s) 43, 76, 302, 305
FspBI CTAG 1 cut(s) 376
GluI GCNGC 4 cut(s) 43, 76, 302, 305
GsaI CCCAGC 1 cut(s) 75
HaeIII GGCC 5 cut(s) 98, 272, 277, 363, 373
HapII CCGG 4 cut(s) 6, 223, 240, 330
HgaI GACGC 3 cut(s) 184, 185, 223
Hin1I GRCGYC 2 cut(s) 177, 234
Hin1II CATG 3 cut(s) 20, 42, 97
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 1 cut(s) 314
HpaII CCGG 4 cut(s) 6, 223, 240, 330
HphI GGTGA 2 cut(s) 73, 229
Hpy166II GTNNAC 2 cut(s) 283, 352
Hpy188I TCNGA 1 cut(s) 109
Hpy188III TCNNGA 1 cut(s) 6
Hpy8I GTNNAC 2 cut(s) 283, 352
Hpy99I CGWCG 2 cut(s) 125, 200
HpyAV CCTTC 1 cut(s) 178
HpyF10VI GCNNNNNNNGC 5 cut(s) 75, 156, 185, 240, 274
Hsp92I GRCGYC 2 cut(s) 177, 234
Hsp92II CATG 3 cut(s) 20, 42, 97
Kpn2I TCCGGA 1 cut(s) 5
KroI GCCGGC 1 cut(s) 329
KroNI GCCGGC 1 cut(s) 331
Kzo9I GATC 2 cut(s) 134, 249
LguI GCTCTTC 2 cut(s) 258, 314
LmnI GCTCC 2 cut(s) 83, 164
Lsp1109I GCAGC 2 cut(s) 87, 288
LweI GCATC 1 cut(s) 300
MaeI CTAG 1 cut(s) 376
MaeIII GTNAC 2 cut(s) 61, 235
MalI GATC 2 cut(s) 136, 251
MbiI CCGCTC 1 cut(s) 307
MboI GATC 2 cut(s) 134, 249
MboII GAAGA 2 cut(s) 275, 301
MhlI GDGCHC 3 cut(s) 36, 71, 375
MlsI TGGCCA 1 cut(s) 272
MluNI TGGCCA 1 cut(s) 272
MnlI CCTC 9 cut(s) 32, 91, 109, 112, 115, 165, 199, 224, 266
Mox20I TGGCCA 1 cut(s) 272
MroI TCCGGA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 329
MscI TGGCCA 1 cut(s) 272
MseI TTAA 1 cut(s) 321
MslI CAYNNNNRTG 3 cut(s) 92, 242, 296
Msp20I TGGCCA 1 cut(s) 272
MspI CCGG 4 cut(s) 6, 223, 240, 330
MspR9I CCNGG 3 cut(s) 29, 224, 274
MvaI CCWGG 2 cut(s) 29, 274
MvnI CGCG 2 cut(s) 195, 261
MwoI GCNNNNNNNGC 5 cut(s) 75, 156, 185, 240, 274
NaeI GCCGGC 1 cut(s) 331
NciI CCSGG 1 cut(s) 224
NcoI CCATGG 1 cut(s) 93
NdeII GATC 2 cut(s) 134, 249
NgoMIV GCCGGC 1 cut(s) 329
NlaIII CATG 3 cut(s) 20, 42, 97
NlaIV GGNNCC 1 cut(s) 373
NmuCI GTSAC 2 cut(s) 61, 235
OliI CACNNNNGTG 1 cut(s) 242
PceI AGGCCT 1 cut(s) 277
PciSI GCTCTTC 2 cut(s) 258, 314
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 258
PdiI GCCGGC 1 cut(s) 331
PfeI GAWTC 1 cut(s) 314
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 4 cut(s) 44, 77, 303, 306
Psp6I CCWGG 2 cut(s) 27, 272
PspFI CCCAGC 1 cut(s) 71
PspGI CCWGG 2 cut(s) 27, 272
PspN4I GGNNCC 1 cut(s) 373
PspOMI GGGCCC 1 cut(s) 371
PspPI GGNCC 2 cut(s) 371, 372
RseI CAYNNNNRTG 3 cut(s) 92, 242, 296
SapI GCTCTTC 2 cut(s) 258, 314
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 4 cut(s) 43, 76, 302, 305
Sau3AI GATC 2 cut(s) 134, 249
Sau96I GGNCC 2 cut(s) 371, 372
ScrFI CCNGG 3 cut(s) 29, 224, 274
SduI GDGCHC 3 cut(s) 36, 71, 375
SetI ASST 8 cut(s) 63, 80, 170, 186, 210, 258, 270, 360
SfaNI GCATC 1 cut(s) 300
SgrAI CRCCGGYG 1 cut(s) 239
SmiMI CAYNNNNRTG 3 cut(s) 92, 242, 296
SseBI AGGCCT 1 cut(s) 277
SsiI CCGC 7 cut(s) 25, 43, 127, 148, 259, 289, 305
SspMI CTAG 1 cut(s) 376
StuI AGGCCT 1 cut(s) 277
StyD4I CCNGG 3 cut(s) 27, 222, 272
StyI CCWWGG 3 cut(s) 93, 180, 364
TaqI TCGA 3 cut(s) 252, 312, 342
TauI GCSGC 2 cut(s) 45, 307
TfiI GAWTC 1 cut(s) 314
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TseFI GTSAC 2 cut(s) 61, 235
TseI GCWGC 2 cut(s) 75, 301
Tsp45I GTSAC 2 cut(s) 61, 235
TspGWI ACGGA 2 cut(s) 206, 307
XspI CTAG 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.