MD04G1064000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016853
Physical Location & Seq
Forward (+)
8505855 .. 8512785
6931 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1064000.v1.1.491

Sequence Viewer

Length: 366 bp
ATGGGTTTTCACATGGCAGCTGTAGCACTCATCAAACTCAAAGTCGTCACCATTTCCGCCTCTCACGGCAACGCTGTGCTGACTGGGTTGATCTGGCCGTTTGTTCTCAAACTCTCTTTCAGCTTCAGACTTCTTTATCAAACCTACTCCGAATTCAGCCATGCATCGAGGCTCTTCTTTTTCCAGCTCAATCAGATTGCAGTTGCTGGTTTGGATGACGATGCAAATCGTCGTCATCGGCAGCCAGTTACTAGGTTGCAACGAGCTCTGAGGCTGCTGAGACACACTGTGACTCAAGAAAGACAGTTACAGGCCTTGGAGTTGGATGAGCAGAGCATGCATGCCCTCGCCATGGTTGCACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

122

Amino Acids

13.8

Weight (kDa)

10.46

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016853)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g18850
malus_domestica MD04G1064000.v1.1
prunus_persica Prupe.5G069300_v2.0.a1
pyrus_communis pycom06g04880
rosa_chinensis RchiOBHm_Chr7g0203191
rosa_laevigata RLG00000003544
rosa_multiflora Rmu_co8225674.1_g000001 Rmu_sc0010754.1_g000015
rosa_roxburghii Rroxscaffold_3G00253980
rosa_rugosa Rorug07G0075000
rosa_samantha Rh7AG204400 Rh7BG203600 Rh7CG215300 Rh7DG210900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 57
AcoI YGGCCR 1 cut(s) 95
AcsI RAATTY 1 cut(s) 152
AcuI CTGAAG 1 cut(s) 109
AdeI CACNNNGTG 1 cut(s) 289
AfiI CCNNNNNNNGG 1 cut(s) 352
AluBI AGCT 4 cut(s) 20, 123, 187, 266
AluI AGCT 4 cut(s) 20, 123, 187, 266
Alw21I GWGCWC 1 cut(s) 268
Alw26I GTCTC 1 cut(s) 274
AlwNI CAGNNNCTG 1 cut(s) 206
AoxI GGCC 2 cut(s) 95, 312
ApeKI GCWGC 3 cut(s) 17, 241, 274
ApoI RAATTY 1 cut(s) 152
ArsI GACNNNNNNTTYG 2 cut(s) 27, 59
AsuHPI GGTGA 1 cut(s) 40
BanII GRGCYC 1 cut(s) 268
Bbv12I GWGCWC 1 cut(s) 268
BbvI GCAGC 3 cut(s) 29, 253, 261
BceAI ACGGC 2 cut(s) 82, 82
BcoDI GTCTC 1 cut(s) 274
BfaI CTAG 1 cut(s) 252
BfmI CTRYAG 1 cut(s) 21
BisI GCNGC 3 cut(s) 18, 242, 275
BlsI GCNGC 3 cut(s) 19, 243, 276
BmrI ACTGGG 1 cut(s) 93
BmsI GCATC 2 cut(s) 173, 211
BmuI ACTGGG 1 cut(s) 93
BpuEI CTTGAG 1 cut(s) 279
BsaBI GATNNNNATC 1 cut(s) 225
BsaJI CCNNGG 2 cut(s) 315, 351
Bsc4I CCNNNNNNNGG 1 cut(s) 352
Bse1I ACTGG 2 cut(s) 88, 245
Bse8I GATNNNNATC 1 cut(s) 225
BseDI CCNNGG 2 cut(s) 315, 351
BseGI GGATG 2 cut(s) 220, 331
BseJI GATNNNNATC 1 cut(s) 225
BseLI CCNNNNNNNGG 1 cut(s) 352
BseMII CTCAG 2 cut(s) 260, 269
BseNI ACTGG 2 cut(s) 88, 245
BseXI GCAGC 3 cut(s) 29, 253, 261
BshFI GGCC 2 cut(s) 97, 314
BsiHKAI GWGCWC 1 cut(s) 268
BslI CCNNNNNNNGG 1 cut(s) 352
BsmAI GTCTC 1 cut(s) 274
BsnI GGCC 2 cut(s) 97, 314
Bsp1286I GDGCHC 1 cut(s) 268
Bsp143I GATC 1 cut(s) 90
Bsp19I CCATGG 1 cut(s) 351
BspACI CCGC 1 cut(s) 57
BspANI GGCC 2 cut(s) 97, 314
BspCNI CTCAG 2 cut(s) 261, 270
BspQI GCTCTTC 1 cut(s) 179
BsrI ACTGG 2 cut(s) 88, 245
BssECI CCNNGG 2 cut(s) 315, 351
BssMI GATC 1 cut(s) 90
BssT1I CCWWGG 2 cut(s) 315, 351
Bst4CI ACNGT 2 cut(s) 289, 306
Bst6I CTCTTC 1 cut(s) 179
BstAPI GCANNNNNTGC 1 cut(s) 337
BstC8I GCNNGC 2 cut(s) 338, 342
BstDEI CTNAG 2 cut(s) 269, 278
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 2 cut(s) 220, 331
BstKTI GATC 1 cut(s) 93
BstMAI GTCTC 1 cut(s) 274
BstMBI GATC 1 cut(s) 90
BstMWI GCNNNNNNNGC 3 cut(s) 23, 337, 356
BstNSI RCATGY 2 cut(s) 340, 344
BstSFI CTRYAG 1 cut(s) 21
BstV1I GCAGC 3 cut(s) 29, 253, 261
BsuRI GGCC 2 cut(s) 97, 314
BtgI CCRYGG 1 cut(s) 351
BtsCI GGATG 2 cut(s) 220, 331
BtsIMutI CAGTG 1 cut(s) 285
Cac8I GCNNGC 2 cut(s) 338, 342
CaiI CAGNNNCTG 1 cut(s) 206
CviAII CATG 5 cut(s) 13, 161, 337, 341, 352
DdeI CTNAG 2 cut(s) 269, 278
DpnI GATC 1 cut(s) 92
DpnII GATC 1 cut(s) 90
DraIII CACNNNGTG 1 cut(s) 289
EaeI YGGCCR 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 179
EarI CTCTTC 1 cut(s) 179
EciI GGCGGA 1 cut(s) 46
Ecl136II GAGCTC 1 cut(s) 266
Eco130I CCWWGG 2 cut(s) 315, 351
Eco147I AGGCCT 1 cut(s) 314
Eco24I GRGCYC 1 cut(s) 268
Eco53kI GAGCTC 1 cut(s) 266
Eco57I CTGAAG 1 cut(s) 109
EcoICRI GAGCTC 1 cut(s) 266
EcoRI GAATTC 1 cut(s) 152
EcoT14I CCWWGG 2 cut(s) 315, 351
EcoT22I ATGCAT 2 cut(s) 166, 342
EcoT38I GRGCYC 1 cut(s) 268
ErhI CCWWGG 2 cut(s) 315, 351
FaeI CATG 5 cut(s) 16, 164, 340, 344, 355
FaiI YATR 5 cut(s) 14, 162, 338, 342, 353
FatI CATG 5 cut(s) 12, 160, 336, 340, 351
Fnu4HI GCNGC 3 cut(s) 18, 242, 275
FokI GGATG 2 cut(s) 227, 338
FriOI GRGCYC 1 cut(s) 268
Fsp4HI GCNGC 3 cut(s) 18, 242, 275
FspBI CTAG 1 cut(s) 252
GluI GCNGC 3 cut(s) 18, 242, 275
HaeIII GGCC 2 cut(s) 97, 314
Hin1II CATG 5 cut(s) 16, 164, 340, 344, 355
HinfI GANTC 1 cut(s) 292
HphI GGTGA 1 cut(s) 40
Hpy188I TCNGA 4 cut(s) 128, 151, 195, 270
Hpy188III TCNNGA 1 cut(s) 296
Hpy99I CGWCG 1 cut(s) 234
HpyCH4III ACNGT 2 cut(s) 289, 306
HpyCH4V TGCA 6 cut(s) 164, 200, 224, 259, 340, 359
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 337, 356
HpyF3I CTNAG 2 cut(s) 269, 278
Hsp92II CATG 5 cut(s) 16, 164, 340, 344, 355
Kzo9I GATC 1 cut(s) 90
LguI GCTCTTC 1 cut(s) 179
LpnPI CCDG 6 cut(s) 69, 79, 192, 197, 258, 296
Lsp1109I GCAGC 3 cut(s) 29, 253, 261
LweI GCATC 2 cut(s) 173, 211
MaeI CTAG 1 cut(s) 252
MaeIII GTNAC 4 cut(s) 46, 247, 289, 306
MalI GATC 1 cut(s) 92
MboI GATC 1 cut(s) 90
MboII GAAGA 1 cut(s) 166
MhlI GDGCHC 1 cut(s) 268
MluCI AATT 1 cut(s) 152
MlyI GAGTC 1 cut(s) 286
MmeI TCCRAC 1 cut(s) 303
MnlI CCTC 4 cut(s) 70, 162, 264, 356
Mph1103I ATGCAT 2 cut(s) 166, 342
MspA1I CMGCKG 1 cut(s) 20
MwoI GCNNNNNNNGC 3 cut(s) 23, 337, 356
NcoI CCATGG 1 cut(s) 351
NdeII GATC 1 cut(s) 90
NlaIII CATG 5 cut(s) 16, 164, 340, 344, 355
NmuCI GTSAC 2 cut(s) 46, 289
NsiI ATGCAT 2 cut(s) 166, 342
NspI RCATGY 2 cut(s) 340, 344
PaeI GCATGC 2 cut(s) 340, 344
PceI AGGCCT 1 cut(s) 314
PciSI GCTCTTC 1 cut(s) 179
PkrI GCNGC 3 cut(s) 19, 243, 276
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
Psp124BI GAGCTC 1 cut(s) 268
PstNI CAGNNNCTG 1 cut(s) 206
PvuII CAGCTG 1 cut(s) 20
SacI GAGCTC 1 cut(s) 268
SapI GCTCTTC 1 cut(s) 179
SatI GCNGC 3 cut(s) 18, 242, 275
Sau3AI GATC 1 cut(s) 90
SchI GAGTC 1 cut(s) 286
SduI GDGCHC 1 cut(s) 268
SetI ASST 6 cut(s) 22, 125, 146, 189, 257, 268
SfaNI GCATC 2 cut(s) 173, 211
SfcI CTRYAG 1 cut(s) 21
SmlI CTYRAG 1 cut(s) 294
SmoI CTYRAG 1 cut(s) 294
SphI GCATGC 2 cut(s) 340, 344
Sse9I AATT 1 cut(s) 152
SseBI AGGCCT 1 cut(s) 314
SsiI CCGC 1 cut(s) 57
SspMI CTAG 1 cut(s) 252
SstI GAGCTC 1 cut(s) 268
StuI AGGCCT 1 cut(s) 314
StyI CCWWGG 2 cut(s) 315, 351
TaaI ACNGT 2 cut(s) 289, 306
TaqI TCGA 1 cut(s) 167
TasI AATT 1 cut(s) 152
TscAI CASTG 1 cut(s) 292
TseFI GTSAC 2 cut(s) 46, 289
TseI GCWGC 3 cut(s) 17, 241, 274
Tsp45I GTSAC 2 cut(s) 46, 289
TspRI CASTG 1 cut(s) 292
XapI RAATTY 1 cut(s) 152
XceI RCATGY 2 cut(s) 340, 344
XspI CTAG 1 cut(s) 252
Zsp2I ATGCAT 2 cut(s) 166, 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.