MD04G1100000.v1.1

Fasciclin-like arabinogalactan protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016223
Physical Location & Seq
Forward (+)
18492811 .. 18493551
741 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1100000.v1.1.491

Sequence Viewer

Length: 741 bp
ATGGCACCCCACTCTTTCTTCGTTTTGCTAGTCTTCTTCCTCTATTCATTTTCATCCAATATTTCAGCTCAGTCTCCGGCAGCAGCCCCCGCCCCATCCGCCACCAACGTGACTGCTGTCCTTGAGAAGGCCGGCCAATTCACCACTTTGATCAAGCTCCTCAAGTCCACCAAAATGGACGACCAAATCAACACCCAACTCAGCACCTCAAACCAAGGGATCACCATCTTTGCACCAACTGACAACGCCTTCTCCAGCCTCAAAGCCGGCACGCTAAACTCGATTTCCGAGCAGCAGAAGCTGCAGCTGGTGCAGTTCCATATCCTGCCATCTTTCTACTCTGCTGCACAATTCCAAACCGTGTCCAACCCTCTGCACACGCAGGCTGGGAACAGCGATGACGGGCAGTTCCCGTTGAATGTGACAACGTCAGGGAACCTAGTGAACATAACAACAGGGGTGGTGAATGCAACCGTGGCTAATACCATTTTTACTGACAGTCAGCTGGCTGTGTATGAGGTCGACCAGGTGCTCCTTCCTTTGAGCATTTTTGGCCCTGCAGCACCCGCTCCTGCGCCATCTAAGCCCGAAACAAAAGTTAAAGGTGCTGATTCACCTTCAGGGTCCTCGGATAGCGGCAGCACTGCTGATTCTTCTGCTGCAATGGGGCTGGAACAGCATGGGATTGCAGAAGCAGCATCGGTGGGGATGATAGCAATTCTTGCAGCATTTTTGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009832 GO:0009834 GO:0009987 GO:0016020 GO:0031224 GO:0031225 GO:0042546 GO:0044085 GO:0044425 GO:0044464 GO:0071554 GO:0071669 GO:0071840 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fasciclin

Protein Analysis

247

Amino Acids

25.53

Weight (kDa)

4.82

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fasciclin PF02469 46 - 180 9.4e-22 Fasciclin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016223)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03170
fragaria_vesca FvH4_6g15700
malus_domestica MD04G1100000.v1.1
prunus_persica Prupe.6G238300_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0469401
rosa_laevigata RLG00000024352
rosa_multiflora Rmu_co8311235.1_g000001 Rmu_sc0005274.1_g000010
rosa_roxburghii Rroxscaffold_6G00411920
rosa_rugosa Rorug03G0101500
rosa_samantha Rh3AG150500 Rh3BG174200 Rh3CG165200 Rh3DG201300
rosa_wichuraiana Rw3G014190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccBSI CCGCTC 1 cut(s) 569
AccI GTMKAC 1 cut(s) 522
AciI CCGC 4 cut(s) 90, 99, 567, 636
AclWI GGATC 1 cut(s) 227
AcoI YGGCCR 1 cut(s) 133
AcuI CTGAAG 1 cut(s) 603
AfiI CCNNNNNNNGG 1 cut(s) 127
AgsI TTSAA 1 cut(s) 418
AjnI CCWGG 1 cut(s) 525
AleI CACNNNNGTG 1 cut(s) 107
AluBI AGCT 5 cut(s) 68, 157, 301, 307, 505
AluI AGCT 5 cut(s) 68, 157, 301, 307, 505
Alw21I GWGCWC 1 cut(s) 534
Alw26I GTCTC 1 cut(s) 78
AlwI GGATC 1 cut(s) 227
AlwNI CAGNNNCTG 1 cut(s) 301
AoxI GGCC 3 cut(s) 129, 133, 553
AspLEI GCGC 1 cut(s) 577
AspS9I GGNCC 2 cut(s) 554, 624
AsuHPI GGTGA 4 cut(s) 133, 214, 475, 606
AvaII GGWCC 1 cut(s) 624
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 534
BccI CCATC 4 cut(s) 103, 233, 337, 586
BciT130I CCWGG 1 cut(s) 527
BclI TGATCA 1 cut(s) 150
BcoDI GTCTC 1 cut(s) 78
BfaI CTAG 2 cut(s) 29, 440
BfmI CTRYAG 2 cut(s) 302, 558
Bme1390I CCNGG 1 cut(s) 527
Bme18I GGWCC 1 cut(s) 624
BmgT120I GGNCC 2 cut(s) 554, 624
BmiI GGNNCC 3 cut(s) 6, 437, 625
BmrFI CCNGG 1 cut(s) 527
BmsI GCATC 1 cut(s) 707
BoxI GACNNNNGTC 1 cut(s) 116
BpiI GAAGAC 1 cut(s) 25
BpmI CTGGAG 1 cut(s) 238
BpuEI CTTGAG 2 cut(s) 143, 146
BsaBI GATNNNNATC 1 cut(s) 224
BsaJI CCNNGG 3 cut(s) 214, 474, 627
BsaXI ACNNNNNCTCC 2 cut(s) 236, 266
Bsc4I CCNNNNNNNGG 1 cut(s) 127
Bse118I RCCGGY 2 cut(s) 131, 266
Bse3DI GCAATG 1 cut(s) 669
Bse8I GATNNNNATC 1 cut(s) 224
BseBI CCWGG 1 cut(s) 527
BseDI CCNNGG 3 cut(s) 214, 474, 627
BseGI GGATG 3 cut(s) 53, 95, 714
BseJI GATNNNNATC 1 cut(s) 224
BseLI CCNNNNNNNGG 1 cut(s) 127
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 2 cut(s) 83, 214
BseRI GAGGAG 1 cut(s) 149
BseYI CCCAGC 1 cut(s) 386
BsgI GTGCAG 3 cut(s) 330, 332, 359
BshFI GGCC 3 cut(s) 131, 135, 555
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 534
BsiSI CCGG 3 cut(s) 77, 132, 267
BslI CCNNNNNNNGG 1 cut(s) 127
BsmAI GTCTC 1 cut(s) 78
BsmI GAATGC 1 cut(s) 472
BsnI GGCC 3 cut(s) 131, 135, 555
Bsp1286I GDGCHC 1 cut(s) 534
Bsp143I GATC 2 cut(s) 150, 219
BspACI CCGC 4 cut(s) 90, 99, 567, 636
BspANI GGCC 3 cut(s) 131, 135, 555
BspCNI CTCAG 2 cut(s) 82, 213
BspLI GGNNCC 3 cut(s) 6, 437, 625
BspMAI CTGCAG 2 cut(s) 306, 562
BspPI GGATC 1 cut(s) 227
BspT107I GGYRCC 1 cut(s) 4
BsrBI CCGCTC 1 cut(s) 569
BsrDI GCAATG 1 cut(s) 669
BsrFI RCCGGY 2 cut(s) 131, 266
BssAI RCCGGY 2 cut(s) 131, 266
BssECI CCNNGG 3 cut(s) 214, 474, 627
BssMI GATC 2 cut(s) 150, 219
BssT1I CCWWGG 1 cut(s) 214
Bst2UI CCWGG 1 cut(s) 527
Bst4CI ACNGT 3 cut(s) 361, 475, 500
BstAPI GCANNNNNTGC 3 cut(s) 301, 310, 722
BstC8I GCNNGC 5 cut(s) 133, 268, 272, 384, 507
BstDEI CTNAG 3 cut(s) 69, 200, 582
BstDSI CCRYGG 1 cut(s) 474
BstENI CCTNNNNNAGG 1 cut(s) 125
BstF5I GGATG 3 cut(s) 53, 95, 714
BstHHI GCGC 1 cut(s) 577
BstKTI GATC 2 cut(s) 153, 222
BstMAI GTCTC 1 cut(s) 78
BstMBI GATC 2 cut(s) 150, 219
BstNI CCWGG 1 cut(s) 527
BstPAI GACNNNNGTC 1 cut(s) 116
BstSCI CCNGG 1 cut(s) 525
BstSFI CTRYAG 2 cut(s) 302, 558
BstV2I GAAGAC 1 cut(s) 25
BstXI CCANNNNNNTGG 1 cut(s) 175
BsuRI GGCC 3 cut(s) 131, 135, 555
BtgI CCRYGG 1 cut(s) 474
BtgZI GCGATG 1 cut(s) 411
BtsCI GGATG 3 cut(s) 53, 95, 714
BtsI GCAGTG 1 cut(s) 642
BtsIMutI CAGTG 1 cut(s) 642
Cac8I GCNNGC 5 cut(s) 133, 268, 272, 384, 507
CaiI CAGNNNCTG 1 cut(s) 301
CfoI GCGC 1 cut(s) 577
Cfr10I RCCGGY 2 cut(s) 131, 266
Cfr13I GGNCC 2 cut(s) 554, 624
CsiI ACCWGGT 1 cut(s) 525
CspCI CAANNNNNGTGG 2 cut(s) 441, 476
CviAII CATG 1 cut(s) 680
DdeI CTNAG 3 cut(s) 69, 200, 582
DpnI GATC 2 cut(s) 152, 221
DpnII GATC 2 cut(s) 150, 219
EaeI YGGCCR 1 cut(s) 133
EciI GGCGGA 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 214
Eco47I GGWCC 1 cut(s) 624
Eco57I CTGAAG 1 cut(s) 603
EcoNI CCTNNNNNAGG 1 cut(s) 125
EcoO109I RGGNCCY 1 cut(s) 624
EcoRII CCWGG 1 cut(s) 525
EcoT14I CCWWGG 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 214
FaeI CATG 1 cut(s) 683
FaiI YATR 4 cut(s) 321, 449, 516, 681
FatI CATG 1 cut(s) 679
FauI CCCGC 2 cut(s) 97, 574
FbaI TGATCA 1 cut(s) 150
FblI GTMKAC 1 cut(s) 522
FokI GGATG 3 cut(s) 40, 82, 721
FseI GGCCGGCC 1 cut(s) 135
FspBI CTAG 2 cut(s) 29, 440
GlaI GCGC 1 cut(s) 576
GsaI CCCAGC 1 cut(s) 390
GsuI CTGGAG 1 cut(s) 238
HaeIII GGCC 3 cut(s) 131, 135, 555
HapII CCGG 3 cut(s) 77, 132, 267
HhaI GCGC 1 cut(s) 577
Hin1II CATG 1 cut(s) 683
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HinfI GANTC 2 cut(s) 611, 650
HpaII CCGG 3 cut(s) 77, 132, 267
HphI GGTGA 4 cut(s) 133, 214, 475, 606
Hpy166II GTNNAC 3 cut(s) 168, 445, 523
Hpy188I TCNGA 2 cut(s) 289, 631
Hpy8I GTNNAC 3 cut(s) 168, 445, 523
HpyAV CCTTC 4 cut(s) 121, 259, 545, 627
HpyCH4III ACNGT 3 cut(s) 361, 475, 500
HpyCH4IV ACGT 2 cut(s) 108, 428
HpyF3I CTNAG 3 cut(s) 69, 200, 582
HpySE526I ACGT 2 cut(s) 108, 428
Hsp92II CATG 1 cut(s) 683
HspAI GCGC 1 cut(s) 575
KroI GCCGGC 2 cut(s) 131, 266
KroNI GCCGGC 2 cut(s) 133, 268
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 2 cut(s) 150, 219
LmnI GCTCC 3 cut(s) 162, 537, 574
LweI GCATC 1 cut(s) 707
MabI ACCWGGT 1 cut(s) 525
MaeI CTAG 2 cut(s) 29, 440
MaeII ACGT 2 cut(s) 108, 428
MaeIII GTNAC 2 cut(s) 109, 421
MalI GATC 2 cut(s) 152, 221
MbiI CCGCTC 1 cut(s) 569
MboI GATC 2 cut(s) 150, 219
MboII GAAGA 4 cut(s) 10, 25, 28, 645
MhlI GDGCHC 1 cut(s) 534
MluCI AATT 3 cut(s) 137, 350, 717
MmeI TCCRAC 1 cut(s) 390
MnlI CCTC 7 cut(s) 50, 170, 217, 269, 381, 511, 637
MroNI GCCGGC 2 cut(s) 131, 266
MseI TTAA 1 cut(s) 600
MslI CAYNNNNRTG 2 cut(s) 107, 173
MspA1I CMGCKG 2 cut(s) 307, 505
MspI CCGG 3 cut(s) 77, 132, 267
MspR9I CCNGG 1 cut(s) 527
Mva1269I GAATGC 1 cut(s) 472
MvaI CCWGG 1 cut(s) 527
NaeI GCCGGC 2 cut(s) 133, 268
NdeII GATC 2 cut(s) 150, 219
NgoMIV GCCGGC 2 cut(s) 131, 266
NlaIII CATG 1 cut(s) 683
NlaIV GGNNCC 3 cut(s) 6, 437, 625
NmuCI GTSAC 2 cut(s) 109, 421
OliI CACNNNNGTG 1 cut(s) 107
PcsI WCGNNNNNNNCGW 1 cut(s) 278
PctI GAATGC 1 cut(s) 472
PdiI GCCGGC 2 cut(s) 133, 268
PfeI GAWTC 2 cut(s) 611, 650
PflFI GACNNNGTC 1 cut(s) 427
PpuMI RGGWCCY 1 cut(s) 624
PshAI GACNNNNGTC 1 cut(s) 116
Psp5II RGGWCCY 1 cut(s) 624
Psp6I CCWGG 1 cut(s) 525
PspFI CCCAGC 1 cut(s) 386
PspGI CCWGG 1 cut(s) 525
PspN4I GGNNCC 3 cut(s) 6, 437, 625
PspPI GGNCC 2 cut(s) 554, 624
PspPPI RGGWCCY 1 cut(s) 624
PstI CTGCAG 2 cut(s) 306, 562
PstNI CAGNNNCTG 1 cut(s) 301
PsyI GACNNNGTC 1 cut(s) 427
PvuII CAGCTG 2 cut(s) 307, 505
RigI GGCCGGCC 1 cut(s) 135
RseI CAYNNNNRTG 2 cut(s) 107, 173
SalI GTCGAC 1 cut(s) 521
SaqAI TTAA 1 cut(s) 600
Sau3AI GATC 2 cut(s) 150, 219
Sau96I GGNCC 2 cut(s) 554, 624
ScrFI CCNGG 1 cut(s) 527
SduI GDGCHC 1 cut(s) 534
SexAI ACCWGGT 1 cut(s) 525
SfaNI GCATC 1 cut(s) 707
SfcI CTRYAG 2 cut(s) 302, 558
SinI GGWCC 1 cut(s) 624
SmiMI CAYNNNNRTG 2 cut(s) 107, 173
SmlI CTYRAG 2 cut(s) 122, 161
SmoI CTYRAG 2 cut(s) 122, 161
Sse9I AATT 3 cut(s) 137, 350, 717
SsiI CCGC 4 cut(s) 90, 99, 567, 636
SspI AATATT 1 cut(s) 61
SspMI CTAG 2 cut(s) 29, 440
StyD4I CCNGG 1 cut(s) 525
StyI CCWWGG 1 cut(s) 214
TaaI ACNGT 3 cut(s) 361, 475, 500
TaiI ACGT 2 cut(s) 111, 431
TaqI TCGA 2 cut(s) 281, 522
TasI AATT 3 cut(s) 137, 350, 717
TauI GCSGC 1 cut(s) 639
TfiI GAWTC 2 cut(s) 611, 650
Tru1I TTAA 1 cut(s) 600
Tru9I TTAA 1 cut(s) 600
TscAI CASTG 1 cut(s) 649
TseFI GTSAC 2 cut(s) 109, 421
Tsp45I GTSAC 2 cut(s) 109, 421
TspDTI ATGAA 2 cut(s) 36, 42
TspRI CASTG 1 cut(s) 649
Tth111I GACNNNGTC 1 cut(s) 427
VpaK11BI GGWCC 1 cut(s) 624
XagI CCTNNNNNAGG 1 cut(s) 125
XmiI GTMKAC 1 cut(s) 522
XspI CTAG 2 cut(s) 29, 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.