MD04G1102000.v1.1

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0015958
Physical Location & Seq
Forward (+)
18858382 .. 18860532
2151 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1102000.v1.1.491

Sequence Viewer

Length: 2151 bp
ATGTTCGATTTTCACTGCTTCCTTTACATTCTGATTCTCTGCATTCCCAAACCCGCCGTGTTCACCGTCGTCGCCGCCACCGAATTTGACTTTGGCACCTTGACTCTGAGCAGCCTGAAGCTCATTGGCGATGCCCACCTGAGCAATGGGAGTGTGCGGCTCACCCGCGACCTCGCCGTTCCCAACTCTGGCGCCGGCCGCGTTTTATACTCCAAACCCATTCGCTTCCGCCAGCCTTCCTCCCCAGTCCCGGCGAGCTTCTCCACATTCTTCTCATTCTCCGTCACCAACCTCAACCCCTCCTCCATCGGCGGCGGTCTCGCGTTCCTCATCTCCCCCGACGACGTCGCTCTCGGCGACGCCGGCGGGTTCCTCGGCCTTCAGTCGGGTCCAGATACTGGGTCGGGTTTTGTGGCCGTCGAATTCGACACGCTCATGGACGTCGAGTTCAAGGACATCAACGGGAACCACGTCGGGTTGGATCTGGACTCCATGGTGTCGACACGTGTCAGCGATCTTGATGCCGTCGACATCGATCTCAAGAGCGGCGATCTGGTGAACTCGTGGATCGAGTACGACGGGTCGAGTCAGGTCATCACCGTCTCGGTTTCATACTCGAATCTGAAACCCAAAGACCCGGTTCTATCATTCTCCCTCGATCTAGCCCAGTACGTCAGCGATTTTATGTACGTTGGGTTTTCCGGGTCGACCCAGGGAAGCACGGAGATTCACAGCGTCGAGTGGTGGAGCTTCAGCTCGTCGTTCGATTCCCCTGTTTCGCCATCCGGGTCGCCGTCTCCGCCGCCGCCGAGCACTACTTTGATGAACCCAACTGCCAATTCAGTAAAATCTCCACCACCGTCAATGCCGCCAAGTGGGTCTTCTTCTAGTAATGCTAGCGGTACTAATCAGAAGAACAGCAAGTCGTCTTCTTCCTGCCATAACCAGCTCTGCAGAGAAGGTCCGGGAGCCGTAGCCGGAGTTGTCACCGCTTCCGCTTTTGTTTTGGCTCTGTTTGCAGGTGTTTTAATCTGGGTTTACTCCAAGAAAGTCAAACAAGTCAAGAAATCGGATTCGAATTTCGCTTCGGATATCATCAAAATGCCGAAGGAGTTCACTTACAGGGAGCTCAAGGCAGCAACTAAGTGCTTCAATGCCAATAGAATCATCGGGCATGGTGCTTTCGGAACAGTCTACAAAGGCATATTATCCGACACCGGCGACATTGTCGCGGTGAAGAGGTGCAGCCACAGCAGTCAGGGGAAGAACGAGTTCTTGTCGGAATTGTCGATAATTGGAACTCTCAGGCACCGGAATTTGGTGAGGCTCCAAGGCTGGTGCCACGAGAAGGGCGAAATTTTGTTGGTGTATGATTTAATGCCGAATGGCAGTCTCGACAAGGCATTGTTCGAGGCAAGAACGCCGCTGCCATGGCCGCACCGGCGAAAAATTCTATTGGGAGTTGCCTCTGCTCTGGCCTATTTGCACCAAGAATGTGAAAATCAGGTGATCCATAGGGACATTAAGACCAGTAACATAATGCTGGATGAAGGGTTCAATGCCCGGTTGGGGGATTTCGGTTTGGCGAGGCAAATCGAGCACGACAAGTCGCCAGACGCCACGGTGGCGGCCGGGACAATGGGGTACCTGGCGCCGGAGTACTTGTTGACAGGCAGAGCTACTGAGAAAACGGATGTGTTTAGCTTTGGGGCGGTGGTGCTTGAGGTGGGGAGTGGGAGGAGGCCAATTGAGAGGGAGGTGAGTGGGGTAGGAAAAGCAGGAGCGTGTAGTAATTTGGTGGAGTGGGTGTGGGGATTGCACAGAGATGGGCGGTTGTTGACGGCGGCGGACCCGAGATTGGAGGGGCAATTTGATGAGGGGGAGATGAGGAAGGTGCTGCTGGTTGGGCTGGCGTGCTCACATCCTGACCCTAATTGTCGGCCAGCAATGAGAGGAGTGGTTCAGATGCTGGTGGGTGAAGCTCAAGTCCCAATTGTGCCAAGAACGAAGCCTTGTACAAGTTTCAGCACTTCACATCTTTTGTTGAGCTTGCAGGACAGTGTTTCTGACTGCAATGGCATGATCACCATCTCCACCTCCTCCTCCGAACTCAGCTTCGGCGCCGGCGCCGGCGGTGATCACATCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase S1FA

Protein Analysis

717

Amino Acids

76.71

Weight (kDa)

5.96

Isoelectric Point (pI)

36.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 27 - 258 2e-61 Legume lectin domain
Lectin_L-type_dom PF18483 39 - 247 2.2e-07 Legume lectin beta-barrel domain
Pkinase PF00069 385 - 586 5.1e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 386 - 584 7.4e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53380
fragaria_vesca FvH4_6g15560
malus_domestica MD04G1102000.v1.1 MD12G1121700.v1.1
prunus_persica Prupe.6G239500_v2.0.a1
pyrus_communis pycom04g09730 pycom12g12070
rosa_chinensis RchiOBHm_Chr3g0469121
rosa_laevigata RLG00000024371
rosa_multiflora Rmu_sc0000302.1_g000014 Rmu_sc0007933.1_g000020
rosa_roxburghii Rroxscaffold_6G00412190
rosa_rugosa Rorug03G0099900
rosa_samantha Rh3AG148900
rosa_wichuraiana Rw3G014050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1010
AatII GACGTC 2 cut(s) 348, 444
Acc36I ACCTGC 1 cut(s) 1010
Acc65I GGTACC 1 cut(s) 1644
AccB1I GGYRCC 8 cut(s) 95, 191, 1308, 1338, 1644, 1651, 2120, 2126
AccB7I CCANNNNNTGG 1 cut(s) 398
AccBSI CCGCTC 1 cut(s) 546
AccI GTMKAC 4 cut(s) 500, 528, 707, 1194
AccII CGCG 4 cut(s) 168, 201, 323, 1232
AclWI GGATC 3 cut(s) 489, 575, 1504
AcoI YGGCCR 5 cut(s) 196, 414, 1433, 1629, 1940
AcsI RAATTY 6 cut(s) 83, 422, 1078, 1315, 1356, 1449
AcuI CTGAAG 3 cut(s) 137, 365, 736
AcvI CACGTG 1 cut(s) 506
AcyI GRCGYC 8 cut(s) 192, 345, 360, 441, 1617, 1652, 2121, 2127
AfaI GTAC 7 cut(s) 575, 671, 689, 904, 1646, 1661, 2017
AflIII ACRYGT 2 cut(s) 503, 505
AgsI TTSAA 3 cut(s) 451, 1153, 1558
AjiI CACGTC 1 cut(s) 472
AjnI CCWGG 2 cut(s) 711, 1647
AjuI GAANNNNNNNTTGG 6 cut(s) 75, 107, 823, 855, 865, 897
AloI GAACNNNNNNTCC 4 cut(s) 431, 463, 1538, 1570
Alw21I GWGCWC 4 cut(s) 815, 1131, 1602, 1919
Alw26I GTCTC 4 cut(s) 323, 607, 801, 1397
AlwI GGATC 3 cut(s) 489, 575, 1504
AlwNI CAGNNNCTG 2 cut(s) 398, 1969
Ama87I CYCGRG 1 cut(s) 1852
AoxI GGCC 8 cut(s) 196, 376, 414, 1433, 1476, 1629, 1742, 1940
ApeKI GCWGC 5 cut(s) 111, 1136, 1245, 1426, 1897
ApoI RAATTY 6 cut(s) 83, 422, 1078, 1315, 1356, 1449
Asp700I GAANNNNTTC 2 cut(s) 1112, 1271
Asp718I GGTACC 1 cut(s) 1644
AspLEI GCGC 4 cut(s) 194, 1654, 2123, 2129
AspS9I GGNCC 3 cut(s) 389, 962, 1849
AsuC2I CCSGG 7 cut(s) 251, 638, 703, 787, 966, 1564, 1633
AsuII TTCGAA 1 cut(s) 1076
AsuNHI GCTAGC 1 cut(s) 896
AvaI CYCGRG 1 cut(s) 1852
AvaII GGWCC 3 cut(s) 389, 962, 1849
BanI GGYRCC 8 cut(s) 95, 191, 1308, 1338, 1644, 1651, 2120, 2126
BanII GRGCYC 1 cut(s) 1131
BauI CACGAG 2 cut(s) 562, 1343
BbrPI CACGTG 1 cut(s) 506
BbsI GAAGAC 2 cut(s) 873, 921
Bbv12I GWGCWC 4 cut(s) 815, 1131, 1602, 1919
BbvI GCAGC 5 cut(s) 123, 1148, 1257, 1413, 1884
BccI CCATC 4 cut(s) 314, 790, 1820, 2096
BceAI ACGGC 7 cut(s) 41, 161, 401, 509, 778, 956, 1857
BcgI CGANNNNNNTGC 2 cut(s) 503, 537
BciT130I CCWGG 2 cut(s) 713, 1649
BclI TGATCA 2 cut(s) 2082, 2137
BcnI CCSGG 7 cut(s) 251, 638, 703, 787, 966, 1564, 1633
BcoDI GTCTC 4 cut(s) 323, 607, 801, 1397
BfaI CTAG 3 cut(s) 662, 888, 897
BfmI CTRYAG 1 cut(s) 952
BfoI RGCGCY 4 cut(s) 195, 1655, 2124, 2130
BfuAI ACCTGC 1 cut(s) 1010
BglI GCCNNNNNGGC 2 cut(s) 1441, 1625
BmcAI AGTACT 1 cut(s) 1661
Bme1390I CCNGG 9 cut(s) 251, 638, 703, 713, 787, 966, 1564, 1633, 1649
Bme18I GGWCC 3 cut(s) 389, 962, 1849
BmeT110I CYCGRG 1 cut(s) 1852
BmgBI CACGTC 1 cut(s) 472
BmgT120I GGNCC 3 cut(s) 389, 962, 1849
BmrFI CCNGG 9 cut(s) 251, 638, 703, 713, 787, 966, 1564, 1633, 1649
BmrI ACTGGG 3 cut(s) 239, 408, 661
BmsI GCATC 3 cut(s) 121, 511, 1956
BmtI GCTAGC 1 cut(s) 900
BmuI ACTGGG 3 cut(s) 239, 408, 661
BoxI GACNNNNGTC 1 cut(s) 506
BpiI GAAGAC 2 cut(s) 873, 921
Bpu10I CCTNAGC 1 cut(s) 140
Bpu14I TTCGAA 1 cut(s) 1076
BpuEI CTTGAG 4 cut(s) 524, 1115, 1742, 1968
BpuMI CCSGG 7 cut(s) 251, 638, 703, 787, 966, 1564, 1633
Bsa29I ATCGAT 1 cut(s) 534
BsaAI YACGTR 1 cut(s) 506
BsaBI GATNNNNATC 1 cut(s) 2087
BsaHI GRCGYC 8 cut(s) 192, 345, 360, 441, 1617, 1652, 2121, 2127
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 7 cut(s) 373, 492, 711, 712, 1330, 1430, 1620
BsaWI WCCGGW 1 cut(s) 1311
BsaXI ACNNNNNCTCC 6 cut(s) 287, 317, 1649, 1679, 1793, 1823
Bse118I RCCGGY 6 cut(s) 194, 362, 1217, 1440, 2123, 2129
Bse1I ACTGG 4 cut(s) 245, 403, 667, 1530
Bse3DI GCAATG 3 cut(s) 151, 1953, 2080
Bse8I GATNNNNATC 1 cut(s) 2087
BseBI CCWGG 2 cut(s) 713, 1649
BseCI ATCGAT 1 cut(s) 534
BseDI CCNNGG 7 cut(s) 373, 492, 711, 712, 1330, 1430, 1620
BseGI GGATG 4 cut(s) 782, 1552, 1699, 1921
BseJI GATNNNNATC 1 cut(s) 2087
BseMI GCAATG 3 cut(s) 151, 1953, 2080
BseMII CTCAG 5 cut(s) 98, 131, 1318, 1674, 2125
BseNI ACTGG 4 cut(s) 245, 403, 667, 1530
BseRI GAGGAG 5 cut(s) 292, 1753, 1968, 2089, 2092
BseX3I CGGCCG 2 cut(s) 196, 1629
BseXI GCAGC 5 cut(s) 123, 1148, 1257, 1413, 1884
BsgI GTGCAG 1 cut(s) 1264
Bsh1236I CGCG 4 cut(s) 168, 201, 323, 1232
Bsh1285I CGRYCG 2 cut(s) 199, 1632
BshFI GGCC 8 cut(s) 198, 378, 416, 1435, 1478, 1631, 1744, 1942
BshNI GGYRCC 8 cut(s) 95, 191, 1308, 1338, 1644, 1651, 2120, 2126
BshVI ATCGAT 1 cut(s) 534
BsiEI CGRYCG 2 cut(s) 199, 1632
BsiHKAI GWGCWC 4 cut(s) 815, 1131, 1602, 1919
BsiHKCI CYCGRG 1 cut(s) 1852
BslFI GGGAC 4 cut(s) 233, 1532, 1648, 1973
BsmAI GTCTC 4 cut(s) 323, 607, 801, 1397
BsmBI CGTCTC 2 cut(s) 607, 801
BsmFI GGGAC 4 cut(s) 233, 1532, 1648, 1973
BsmI GAATGC 1 cut(s) 42
BsnI GGCC 8 cut(s) 198, 378, 416, 1435, 1478, 1631, 1744, 1942
Bso31I GGTCTC 1 cut(s) 323
BsoBI CYCGRG 1 cut(s) 1852
Bsp119I TTCGAA 1 cut(s) 1076
Bsp1286I GDGCHC 4 cut(s) 815, 1131, 1602, 1919
Bsp1407I TGTACA 1 cut(s) 2015
Bsp143I GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1509, 2082, 2137
Bsp19I CCATGG 2 cut(s) 492, 1430
BspANI GGCC 8 cut(s) 198, 378, 416, 1435, 1478, 1631, 1744, 1942
BspCNI CTCAG 5 cut(s) 99, 132, 1317, 1675, 2124
BspDI ATCGAT 1 cut(s) 534
BspFNI CGCG 4 cut(s) 168, 201, 323, 1232
BspMAI CTGCAG 1 cut(s) 956
BspMI ACCTGC 1 cut(s) 1010
BspOI GCTAGC 1 cut(s) 900
BspPI GGATC 3 cut(s) 489, 575, 1504
BspT104I TTCGAA 1 cut(s) 1076
BspT107I GGYRCC 8 cut(s) 95, 191, 1308, 1338, 1644, 1651, 2120, 2126
BspTNI GGTCTC 1 cut(s) 323
BsrBI CCGCTC 1 cut(s) 546
BsrDI GCAATG 3 cut(s) 151, 1953, 2080
BsrFI RCCGGY 6 cut(s) 194, 362, 1217, 1440, 2123, 2129
BsrGI TGTACA 1 cut(s) 2015
BsrI ACTGG 4 cut(s) 245, 403, 667, 1530
BssAI RCCGGY 6 cut(s) 194, 362, 1217, 1440, 2123, 2129
BssECI CCNNGG 7 cut(s) 373, 492, 711, 712, 1330, 1430, 1620
BssMI GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1509, 2082, 2137
BssNI GRCGYC 8 cut(s) 192, 345, 360, 441, 1617, 1652, 2121, 2127
BssSI CACGAG 2 cut(s) 562, 1343
BssT1I CCWWGG 3 cut(s) 492, 1330, 1430
Bst2BI CACGAG 2 cut(s) 562, 1343
Bst2UI CCWGG 2 cut(s) 713, 1649
Bst4CI ACNGT 6 cut(s) 67, 601, 861, 1192, 1624, 2060
Bst6I CTCTTC 1 cut(s) 1232
BstACI GRCGYC 8 cut(s) 192, 345, 360, 441, 1617, 1652, 2121, 2127
BstAUI TGTACA 1 cut(s) 2015
BstBAI YACGTR 1 cut(s) 506
BstBI TTCGAA 1 cut(s) 1076
BstDEI CTNAG 6 cut(s) 107, 140, 1143, 1304, 1683, 2111
BstDSI CCRYGG 3 cut(s) 492, 1430, 1620
BstF5I GGATG 4 cut(s) 782, 1552, 1699, 1921
BstFNI CGCG 4 cut(s) 168, 201, 323, 1232
BstH2I RGCGCY 4 cut(s) 195, 1655, 2124, 2130
BstHHI GCGC 4 cut(s) 194, 1654, 2123, 2129
BstKTI GATC 9 cut(s) 484, 517, 538, 553, 570, 661, 1512, 2085, 2140
BstMAI GTCTC 4 cut(s) 323, 607, 801, 1397
BstMBI GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1509, 2082, 2137
BstMCI CGRYCG 2 cut(s) 199, 1632
BstNI CCWGG 2 cut(s) 713, 1649
BstPAI GACNNNNGTC 1 cut(s) 506
BstSCI CCNGG 9 cut(s) 249, 636, 701, 711, 785, 964, 1562, 1631, 1647
BstSFI CTRYAG 1 cut(s) 952
BstUI CGCG 4 cut(s) 168, 201, 323, 1232
BstV1I GCAGC 5 cut(s) 123, 1148, 1257, 1413, 1884
BstV2I GAAGAC 2 cut(s) 873, 921
BstX2I RGATCY 1 cut(s) 481
BstYI RGATCY 1 cut(s) 481
BstZI CGGCCG 2 cut(s) 196, 1629
Bsu15I ATCGAT 1 cut(s) 534
BsuRI GGCC 8 cut(s) 198, 378, 416, 1435, 1478, 1631, 1744, 1942
BsuTUI ATCGAT 1 cut(s) 534
BtgI CCRYGG 3 cut(s) 492, 1430, 1620
BtgZI GCGATG 1 cut(s) 144
BtrI CACGTC 1 cut(s) 472
BtsCI GGATG 4 cut(s) 782, 1552, 1699, 1921
BtsI GCAGTG 1 cut(s) 13
BtsIMutI CAGTG 2 cut(s) 13, 2065
BveI ACCTGC 1 cut(s) 1010
CaiI CAGNNNCTG 2 cut(s) 398, 1969
CfoI GCGC 4 cut(s) 194, 1654, 2123, 2129
Cfr10I RCCGGY 6 cut(s) 194, 362, 1217, 1440, 2123, 2129
Cfr13I GGNCC 3 cut(s) 389, 962, 1849
ClaI ATCGAT 1 cut(s) 534
CseI GACGC 3 cut(s) 368, 724, 1625
Csp6I GTAC 7 cut(s) 574, 670, 688, 903, 1645, 1660, 2016
CspCI CAANNNNNGTGG 2 cut(s) 67, 102
CviAII CATG 5 cut(s) 436, 493, 1175, 1431, 2080
CviQI GTAC 7 cut(s) 574, 670, 688, 903, 1645, 1660, 2016
DdeI CTNAG 6 cut(s) 107, 140, 1143, 1304, 1683, 2111
DinI GGCGCC 4 cut(s) 193, 1653, 2122, 2128
DpnI GATC 9 cut(s) 483, 516, 537, 552, 569, 660, 1511, 2084, 2139
DpnII GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1509, 2082, 2137
EaeI YGGCCR 5 cut(s) 196, 414, 1433, 1629, 1940
EagI CGGCCG 2 cut(s) 196, 1629
Eam1104I CTCTTC 1 cut(s) 1232
EarI CTCTTC 1 cut(s) 1232
EciI GGCGGA 3 cut(s) 218, 789, 1862
Ecl136II GAGCTC 1 cut(s) 1129
EclXI CGGCCG 2 cut(s) 196, 1629
Eco130I CCWWGG 3 cut(s) 492, 1330, 1430
Eco24I GRGCYC 1 cut(s) 1131
Eco31I GGTCTC 1 cut(s) 323
Eco32I GATATC 1 cut(s) 1093
Eco47I GGWCC 3 cut(s) 389, 962, 1849
Eco52I CGGCCG 2 cut(s) 196, 1629
Eco53kI GAGCTC 1 cut(s) 1129
Eco57I CTGAAG 3 cut(s) 137, 365, 736
Eco72I CACGTG 1 cut(s) 506
Eco88I CYCGRG 1 cut(s) 1852
EcoICRI GAGCTC 1 cut(s) 1129
EcoRI GAATTC 1 cut(s) 422
EcoRII CCWGG 2 cut(s) 711, 1647
EcoRV GATATC 1 cut(s) 1093
EcoT14I CCWWGG 3 cut(s) 492, 1330, 1430
EcoT38I GRGCYC 1 cut(s) 1131
EgeI GGCGCC 4 cut(s) 193, 1653, 2122, 2128
EheI GGCGCC 4 cut(s) 193, 1653, 2122, 2128
ErhI CCWWGG 3 cut(s) 492, 1330, 1430
Esp3I CGTCTC 2 cut(s) 607, 801
FaeI CATG 5 cut(s) 439, 496, 1178, 1434, 2083
FalI AAGNNNNNCTT 2 cut(s) 865, 897
FaqI GGGAC 4 cut(s) 233, 1532, 1648, 1973
FatI CATG 5 cut(s) 435, 492, 1174, 1430, 2079
FauI CCCGC 3 cut(s) 61, 173, 359
FbaI TGATCA 2 cut(s) 2082, 2137
FblI GTMKAC 4 cut(s) 500, 528, 707, 1194
FokI GGATG 4 cut(s) 769, 1559, 1706, 1908
FriOI GRGCYC 1 cut(s) 1131
FspBI CTAG 3 cut(s) 662, 888, 897
GlaI GCGC 4 cut(s) 193, 1653, 2122, 2128
HaeII RGCGCY 4 cut(s) 195, 1655, 2124, 2130
HaeIII GGCC 8 cut(s) 198, 378, 416, 1435, 1478, 1631, 1744, 1942
HgaI GACGC 3 cut(s) 368, 724, 1625
HhaI GCGC 4 cut(s) 194, 1654, 2123, 2129
Hin1I GRCGYC 8 cut(s) 192, 345, 360, 441, 1617, 1652, 2121, 2127
Hin1II CATG 5 cut(s) 439, 496, 1178, 1434, 2083
Hin6I GCGC 4 cut(s) 192, 1652, 2121, 2127
HinP1I GCGC 4 cut(s) 192, 1652, 2121, 2127
HincII GTYRAC 5 cut(s) 501, 529, 708, 1668, 1839
HindII GTYRAC 5 cut(s) 501, 529, 708, 1668, 1839
HinfI GANTC 9 cut(s) 34, 103, 488, 586, 619, 727, 767, 1073, 1164
Hpy188III TCNNGA 7 cut(s) 392, 485, 518, 541, 1063, 1394, 1925
HpyAV CCTTC 7 cut(s) 246, 389, 953, 1102, 1342, 1544, 1885
HpyCH4III ACNGT 6 cut(s) 67, 601, 861, 1192, 1624, 2060
HpyCH4IV ACGT 6 cut(s) 345, 441, 471, 505, 672, 690
HpyCH4V TGCA 8 cut(s) 42, 954, 1019, 1245, 1486, 1819, 2053, 2073
HpyF3I CTNAG 6 cut(s) 107, 140, 1143, 1304, 1683, 2111
HpySE526I ACGT 6 cut(s) 345, 441, 471, 505, 672, 690
Hsp92I GRCGYC 8 cut(s) 192, 345, 360, 441, 1617, 1652, 2121, 2127
Hsp92II CATG 5 cut(s) 439, 496, 1178, 1434, 2083
HspAI GCGC 4 cut(s) 192, 1652, 2121, 2127
KasI GGCGCC 4 cut(s) 191, 1651, 2120, 2126
KpnI GGTACC 1 cut(s) 1648
KroI GCCGGC 4 cut(s) 194, 362, 2123, 2129
KroNI GCCGGC 4 cut(s) 196, 364, 2125, 2131
Ksp22I TGATCA 2 cut(s) 2082, 2137
Kzo9I GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1509, 2082, 2137
LmnI GCTCC 5 cut(s) 747, 968, 1126, 1332, 1781
Lsp1109I GCAGC 5 cut(s) 123, 1148, 1257, 1413, 1884
LweI GCATC 3 cut(s) 121, 511, 1956
MaeI CTAG 3 cut(s) 662, 888, 897
MaeII ACGT 6 cut(s) 345, 441, 471, 505, 672, 690
MaeIII GTNAC 3 cut(s) 283, 985, 1532
MalI GATC 9 cut(s) 483, 516, 537, 552, 569, 660, 1511, 2084, 2139
MbiI CCGCTC 1 cut(s) 546
MboI GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1509, 2082, 2137
MboII GAAGA 8 cut(s) 262, 873, 876, 921, 924, 925, 1249, 1276
MfeI CAATTG 2 cut(s) 1746, 1992
MflI RGATCY 1 cut(s) 481
MhlI GDGCHC 4 cut(s) 815, 1131, 1602, 1919
Mly113I GGCGCC 4 cut(s) 192, 1652, 2121, 2127
MlyI GAGTC 3 cut(s) 97, 482, 595
MmeI TCCRAC 3 cut(s) 459, 1236, 1260
MreI CGCCGGCG 3 cut(s) 362, 2123, 2129
MroNI GCCGGC 4 cut(s) 194, 362, 2123, 2129
MroXI GAANNNNTTC 2 cut(s) 1112, 1271
MseI TTAA 3 cut(s) 1028, 1376, 1524
MslI CAYNNNNRTG 3 cut(s) 434, 1100, 1824
MspA1I CMGCKG 1 cut(s) 1426
MspR9I CCNGG 9 cut(s) 251, 638, 703, 713, 787, 966, 1564, 1633, 1649
MunI CAATTG 2 cut(s) 1746, 1992
Mva1269I GAATGC 1 cut(s) 42
MvaI CCWGG 2 cut(s) 713, 1649
MvnI CGCG 4 cut(s) 168, 201, 323, 1232
NaeI GCCGGC 4 cut(s) 196, 364, 2125, 2131
NarI GGCGCC 4 cut(s) 192, 1652, 2121, 2127
NciI CCSGG 7 cut(s) 251, 638, 703, 787, 966, 1564, 1633
NcoI CCATGG 2 cut(s) 492, 1430
NdeII GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1509, 2082, 2137
NgoMIV GCCGGC 4 cut(s) 194, 362, 2123, 2129
NheI GCTAGC 1 cut(s) 896
NlaIII CATG 5 cut(s) 439, 496, 1178, 1434, 2083
NmeAIII GCCGAG 3 cut(s) 333, 354, 834
NmuCI GTSAC 2 cut(s) 283, 985
NspV TTCGAA 1 cut(s) 1076
PaqCI CACCTGC 1 cut(s) 1010
PasI CCCWGGG 1 cut(s) 712
PcsI WCGNNNNNNNCGW 5 cut(s) 78, 354, 468, 1286, 1350
PctI GAATGC 1 cut(s) 42
PdiI GCCGGC 4 cut(s) 196, 364, 2125, 2131
PdmI GAANNNNTTC 2 cut(s) 1112, 1271
PfeI GAWTC 6 cut(s) 34, 619, 727, 767, 1073, 1164
PflFI GACNNNGTC 2 cut(s) 344, 1226
PflMI CCANNNNNTGG 1 cut(s) 398
PfoI TCCNGGA 1 cut(s) 964
PleI GAGTC 3 cut(s) 97, 482, 594
PluTI GGCGCC 4 cut(s) 195, 1655, 2124, 2130
PmaCI CACGTG 1 cut(s) 506
PmlI CACGTG 1 cut(s) 506
PpsI GAGTC 3 cut(s) 97, 482, 594
Ppu21I YACGTR 1 cut(s) 506
PshAI GACNNNNGTC 1 cut(s) 506
Psp124BI GAGCTC 1 cut(s) 1131
Psp6I CCWGG 2 cut(s) 711, 1647
PspCI CACGTG 1 cut(s) 506
PspGI CCWGG 2 cut(s) 711, 1647
PspPI GGNCC 3 cut(s) 389, 962, 1849
PstI CTGCAG 1 cut(s) 956
PstNI CAGNNNCTG 2 cut(s) 398, 1969
PsuI RGATCY 1 cut(s) 481
PsyI GACNNNGTC 2 cut(s) 344, 1226
RsaI GTAC 7 cut(s) 575, 671, 689, 904, 1646, 1661, 2017
RsaNI GTAC 7 cut(s) 574, 670, 688, 903, 1645, 1660, 2016
RseI CAYNNNNRTG 3 cut(s) 434, 1100, 1824
SacI GAGCTC 1 cut(s) 1131
SalI GTCGAC 3 cut(s) 499, 527, 706
SaqAI TTAA 3 cut(s) 1028, 1376, 1524
Sau3AI GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1509, 2082, 2137
Sau96I GGNCC 3 cut(s) 389, 962, 1849
ScaI AGTACT 1 cut(s) 1661
SchI GAGTC 3 cut(s) 97, 482, 595
ScrFI CCNGG 9 cut(s) 251, 638, 703, 713, 787, 966, 1564, 1633, 1649
SduI GDGCHC 4 cut(s) 815, 1131, 1602, 1919
SfaNI GCATC 3 cut(s) 121, 511, 1956
SfcI CTRYAG 1 cut(s) 952
SfoI GGCGCC 4 cut(s) 193, 1653, 2122, 2128
SfuI TTCGAA 1 cut(s) 1076
SgrAI CRCCGGYG 5 cut(s) 362, 1217, 1440, 2123, 2129
SinI GGWCC 3 cut(s) 389, 962, 1849
SmiMI CAYNNNNRTG 3 cut(s) 434, 1100, 1824
SmlI CTYRAG 4 cut(s) 539, 1130, 1721, 1983
SmoI CTYRAG 4 cut(s) 539, 1130, 1721, 1983
SspDI GGCGCC 4 cut(s) 191, 1651, 2120, 2126
SspMI CTAG 3 cut(s) 662, 888, 897
SstI GAGCTC 1 cut(s) 1131
StyD4I CCNGG 9 cut(s) 249, 636, 701, 711, 785, 964, 1562, 1631, 1647
StyI CCWWGG 3 cut(s) 492, 1330, 1430
TaaI ACNGT 6 cut(s) 67, 601, 861, 1192, 1624, 2060
TaiI ACGT 6 cut(s) 348, 444, 474, 508, 675, 693
TatI WGTACW 2 cut(s) 1659, 2015
TfiI GAWTC 6 cut(s) 34, 619, 727, 767, 1073, 1164
Tru1I TTAA 3 cut(s) 1028, 1376, 1524
Tru9I TTAA 3 cut(s) 1028, 1376, 1524
TscAI CASTG 2 cut(s) 20, 2065
TseFI GTSAC 2 cut(s) 283, 985
TseI GCWGC 5 cut(s) 111, 1136, 1245, 1426, 1897
Tsp45I GTSAC 2 cut(s) 283, 985
TspDTI ATGAA 3 cut(s) 600, 839, 1563
TspGWI ACGGA 3 cut(s) 271, 737, 1706
TspRI CASTG 2 cut(s) 20, 2065
Tth111I GACNNNGTC 2 cut(s) 344, 1226
Van91I CCANNNNNTGG 1 cut(s) 398
VpaK11BI GGWCC 3 cut(s) 389, 962, 1849
XapI RAATTY 6 cut(s) 83, 422, 1078, 1315, 1356, 1449
XcmI CCANNNNNNNNNTGG 1 cut(s) 143
XmiI GTMKAC 4 cut(s) 500, 528, 707, 1194
XmnI GAANNNNTTC 2 cut(s) 1112, 1271
XspI CTAG 3 cut(s) 662, 888, 897
ZraI GACGTC 2 cut(s) 346, 442
ZrmI AGTACT 1 cut(s) 1661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.