pycom12g12070

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0015958
Physical Location & Seq
Forward (+)
14583930 .. 14586062
2133 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g12070.1

Sequence Viewer

Length: 2133 bp
ATGTTGGATTGTCACTGCTTCCTTTACTTTCTGGTTCTTTGCATTCCGAACGCAGCCGTATTCGCCTTCGCGGCCACCACTGAATTTGACTTCGGCACCTTGACATTGAGCAGCTTGAAGCTCATCGGCGATGCTCACCTGAACAATGGGAGTGTGCGGCTCACCCGCGACCTCACCGTTCCCAACTCCGGCGCCGGCCGCGTTTTATACGCCAAACCCATCCGCTTCCGCCAGCCCTCTTCCCCGTTGCCGGCGAGCTTCTTCACATTCTTCTCATTCTCCGTCACCAATCTTAACCCCTCCTCCATCGGCGGCGGACTCGCTTTCCTCATTTCTTCCGACGACTCCGCCCTTGGAGAAGCGGGCGGGTCCCTCGGCCTACAGTCGGGTCCCGATACCAGGTCGGATTTCGTCGCCGTCGAGTTCGACACACTCATGGACGTCGAGTTCGAAGACATCAACGGGAACCACGTCGGATTGGATCTGAACTCCATGGTTTCGACACGTGTCAGCGATCTGAGTTCCGTCGACATCGATCTCAAGAGCGGCGATCTGGTGAACTCGTCGATCGAGTACGACGGCTCTAGTGGGGTCATCAACGTCTCGGTTTCGTACTCGAATCTGAAACCCACAGACCCGGTTCTATCATTCTCTCTCGATCTGGGCCAGTACGTAAGCGATTTTATGTACGTTGGGTTTTCCGGGTCGACCCAGGGAAGCACGGAGATTCACAGCGTCGAGTGGTGGAGCTTCAGCTCGTCGTTCGATTCCCCTGTTTCGCCATCCGGGTCTCCGCCGCCGCCGACCACCACTCTGATGAATCCAACTGCCAGTTCAGTCAAATCTCCACCGCCGTCAATTCCGCCAAGTGGGTCTTCTTCTAGTAATGCTACCAGTACTAATCAGAAGAACAGCAAGTCGTCTTCTTCCTGCCATAACCATCTCTGCAGAGAAGGTCCGGGAGCCGTCGCCGGAGTTGTCACCGCTTCCGCTTTCGTTTTGGCTCTGTTCGCCGGGGTTTTGATCTGGGTGTACTCCAAGAAAGTCAAACTAGTCAAGAAATCGGATTCGAATATCGCTTCGGATATCATTAAAATGCCGAAGGAGTTCACTTACAGGGAGCTCAAGGCAGCTACCAAGTGCTTCAATGCCAACAGAATCATCGGGCACGGCGCTTTCGGAACAGTCTATAAGGGCATATTATCCGACACGGGCGACATTGTTGCGGTGAAGAGGTGCAGCCACAACAGTCAGGGGAAGAACGAGTTCTTGTCGGAATTGTCGACAATTGGAACTCTCAGGCACCGGAATTTGGTGAGGCTCCAAGGTTGGTGCCACGAGAAGGGCGAAATTTTGTTAGTGTATGAATTGATGCCGAATGGCAGTCTGGACAAAGCACTGTTCGAGGCAAGAACGCCGCTGCCATGGCCTCACAGGCGTAAAATTCTACTGGGAGTTGCCTCTGCTTTGTCCTATATGCACCAAGAGTGCGAAAACCAGGTGATCCATAGAGATATTAAGACCAGCAATATAATGCTGGATGAAGGGTTCAATGCCCGTTTGGGGGATTTCGGTTTGGCGAGGCAAATCGAGCACAATAAGTCCCCGGATGCCACGGTGGCGGCCGGGACAATGGGTTATCTGGCGCCGGATTATTTGTTGACAGGCAGGGCAACTGAGAAGACTGATGTATTTAGCTTTGGGGCGGTGGTGCTGGAGGTGGGGAGTGGGAGGAGGCCAATTGAGAGGGAGGTGAGTGGGGTTGGGAAAGTTGGTGTGTGTAATAACTTGGTGGAGTGGGTGTGGGGATTGCACCGAGATGGGCGGCTGTTGACGGCGGCGGACCCGAGACTGGAGGGGCAATTTGATGAGGGGGAGATGAGGAATGTGTTGTTGGTAGGGCTGGCTTGCTCACATCCTGACCCAAATTGTAGACCGGCAATGAGAGGTGTGGTCCAAATGCTGGTGGGTGAAGCTGAAGTCCCAATCGTGCCAAGAACAAAGCCTTCAACAAGTTTCAGCACCTCACATCTTTTGATGAGTTTGCAGGACACAGTTTCTGAATGCAATGGCATGGTCACGATCTCAACCTCCTCCTCCGAACACAGCTTCGGCGGCGTTCACATCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase S1FA

Protein Analysis

711

Amino Acids

76.3

Weight (kDa)

5.9

Isoelectric Point (pI)

39.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 27 - 258 9.8e-60 Legume lectin domain
Lectin_L-type_dom PF18483 39 - 247 1.3e-07 Legume lectin beta-barrel domain
Pkinase PF00069 383 - 583 1.1e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 384 - 582 2.1e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53380
fragaria_vesca FvH4_6g15560
malus_domestica MD04G1102000.v1.1 MD12G1121700.v1.1
prunus_persica Prupe.6G239500_v2.0.a1
pyrus_communis pycom04g09730 pycom12g12070
rosa_chinensis RchiOBHm_Chr3g0469121
rosa_laevigata RLG00000024371
rosa_multiflora Rmu_sc0000302.1_g000014 Rmu_sc0007933.1_g000020
rosa_roxburghii Rroxscaffold_6G00412190
rosa_rugosa Rorug03G0099900
rosa_samantha Rh3AG148900
rosa_wichuraiana Rw3G014050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 444
AccB1I GGYRCC 5 cut(s) 95, 191, 1302, 1332, 1645
AccB7I CCANNNNNTGG 1 cut(s) 1963
AccBSI CCGCTC 1 cut(s) 546
AccI GTMKAC 4 cut(s) 528, 707, 1283, 1933
AccII CGCG 3 cut(s) 71, 168, 201
AclWI GGATC 2 cut(s) 489, 1498
AcoI YGGCCR 3 cut(s) 72, 196, 1623
AcsI RAATTY 4 cut(s) 83, 1309, 1350, 1443
AcuI CTGAAG 2 cut(s) 736, 1998
AcvI CACGTG 1 cut(s) 506
AcyI GRCGYC 3 cut(s) 192, 441, 1646
AfaI GTAC 6 cut(s) 575, 614, 671, 689, 898, 1034
AflIII ACRYGT 2 cut(s) 503, 505
AgsI TTSAA 4 cut(s) 118, 1147, 1552, 2010
AhlI ACTAGT 1 cut(s) 1051
AjiI CACGTC 1 cut(s) 472
AjnI CCWGG 3 cut(s) 398, 711, 1497
AjuI GAANNNNNNNTTGG 6 cut(s) 817, 849, 859, 891, 1877, 1909
AloI GAACNNNNNNTCC 4 cut(s) 431, 463, 1532, 1564
Alw21I GWGCWC 2 cut(s) 1125, 1596
Alw26I GTCTC 3 cut(s) 607, 795, 1843
AlwI GGATC 2 cut(s) 489, 1498
AlwNI CAGNNNCTG 1 cut(s) 2060
Ama87I CYCGRG 1 cut(s) 1846
AoxI GGCC 7 cut(s) 72, 196, 376, 664, 1427, 1623, 1736
ApeKI GCWGC 5 cut(s) 53, 111, 1130, 1239, 1420
ApoI RAATTY 4 cut(s) 83, 1309, 1350, 1443
ArsI GACNNNNNNTTYG 2 cut(s) 431, 463
Asp700I GAANNNNTTC 2 cut(s) 1106, 1265
AspLEI GCGC 3 cut(s) 194, 1175, 1648
AspS9I GGNCC 6 cut(s) 369, 389, 664, 956, 1843, 1954
AsuC2I CCSGG 7 cut(s) 638, 703, 787, 960, 1015, 1607, 1627
AsuII TTCGAA 2 cut(s) 450, 1070
AvaI CYCGRG 1 cut(s) 1846
AvaII GGWCC 5 cut(s) 369, 389, 956, 1843, 1954
BaeGI GKGCMC 1 cut(s) 1170
BanI GGYRCC 5 cut(s) 95, 191, 1302, 1332, 1645
BanII GRGCYC 1 cut(s) 1125
BauI CACGAG 1 cut(s) 1337
BbrPI CACGTG 1 cut(s) 506
BbsI GAAGAC 4 cut(s) 459, 867, 915, 1688
Bbv12I GWGCWC 2 cut(s) 1125, 1596
BbvI GCAGC 5 cut(s) 65, 123, 1142, 1251, 1407
BccI CCATC 5 cut(s) 227, 314, 790, 948, 1814
BceAI ACGGC 7 cut(s) 41, 401, 595, 838, 950, 1186, 1851
BcgI CGANNNNNNTGC 2 cut(s) 1205, 1239
BciT130I CCWGG 3 cut(s) 400, 713, 1499
BcnI CCSGG 7 cut(s) 638, 703, 787, 960, 1015, 1607, 1627
BcoDI GTCTC 3 cut(s) 607, 795, 1843
BcuI ACTAGT 1 cut(s) 1051
BfaI CTAG 3 cut(s) 585, 882, 1052
BfmI CTRYAG 2 cut(s) 380, 946
BfoI RGCGCY 3 cut(s) 195, 1176, 1649
BglI GCCNNNNNGGC 3 cut(s) 71, 1435, 1619
BmcAI AGTACT 1 cut(s) 898
Bme18I GGWCC 5 cut(s) 369, 389, 956, 1843, 1954
BmeT110I CYCGRG 1 cut(s) 1846
BmgBI CACGTC 1 cut(s) 472
BmgT120I GGNCC 6 cut(s) 369, 389, 664, 956, 1843, 1954
BmrI ACTGGG 1 cut(s) 1460
BmsI GCATC 3 cut(s) 121, 1362, 1600
BmuI ACTGGG 1 cut(s) 1460
BoxI GACNNNNGTC 1 cut(s) 506
BpiI GAAGAC 4 cut(s) 459, 867, 915, 1688
BpmI CTGGAG 2 cut(s) 1736, 1874
Bpu14I TTCGAA 2 cut(s) 450, 1070
BpuEI CTTGAG 2 cut(s) 524, 1109
BpuMI CCSGG 7 cut(s) 638, 703, 787, 960, 1015, 1607, 1627
Bsa29I ATCGAT 1 cut(s) 534
BsaAI YACGTR 2 cut(s) 506, 673
BsaHI GRCGYC 3 cut(s) 192, 441, 1646
BsaI GGTCTC 1 cut(s) 795
BsaWI WCCGGW 1 cut(s) 1305
BsaXI ACNNNNNCTCC 4 cut(s) 287, 317, 1787, 1817
Bse118I RCCGGY 3 cut(s) 194, 250, 1936
Bse1I ACTGG 5 cut(s) 667, 831, 894, 1455, 1857
Bse3DI GCAATG 2 cut(s) 1947, 2074
BseBI CCWGG 3 cut(s) 400, 713, 1499
BseCI ATCGAT 1 cut(s) 534
BseGI GGATG 5 cut(s) 219, 782, 1546, 1615, 1915
BseMI GCAATG 2 cut(s) 1947, 2074
BseMII CTCAG 3 cut(s) 509, 1312, 1668
BseNI ACTGG 5 cut(s) 667, 831, 894, 1455, 1857
BseRI GAGGAG 4 cut(s) 292, 1747, 2083, 2086
BseSI GKGCMC 1 cut(s) 1170
BseX3I CGGCCG 2 cut(s) 196, 1623
BseXI GCAGC 5 cut(s) 65, 123, 1142, 1251, 1407
BsgI GTGCAG 1 cut(s) 1258
Bsh1236I CGCG 3 cut(s) 71, 168, 201
Bsh1285I CGRYCG 3 cut(s) 199, 570, 1626
BshFI GGCC 7 cut(s) 74, 198, 378, 666, 1429, 1625, 1738
BshNI GGYRCC 5 cut(s) 95, 191, 1302, 1332, 1645
BshVI ATCGAT 1 cut(s) 534
BsiEI CGRYCG 3 cut(s) 199, 570, 1626
BsiHKAI GWGCWC 2 cut(s) 1125, 1596
BsiHKCI CYCGRG 1 cut(s) 1846
BslFI GGGAC 5 cut(s) 355, 375, 1588, 1642, 1967
BsmAI GTCTC 3 cut(s) 607, 795, 1843
BsmBI CGTCTC 1 cut(s) 607
BsmFI GGGAC 5 cut(s) 355, 375, 1588, 1642, 1967
BsmI GAATGC 2 cut(s) 42, 2069
BsnI GGCC 7 cut(s) 74, 198, 378, 666, 1429, 1625, 1738
Bso31I GGTCTC 1 cut(s) 795
BsoBI CYCGRG 1 cut(s) 1846
Bsp119I TTCGAA 2 cut(s) 450, 1070
Bsp1286I GDGCHC 3 cut(s) 1125, 1170, 1596
Bsp143I GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1023, 1503, 2082
Bsp19I CCATGG 2 cut(s) 492, 1424
BspANI GGCC 7 cut(s) 74, 198, 378, 666, 1429, 1625, 1738
BspCNI CTCAG 3 cut(s) 510, 1311, 1669
BspDI ATCGAT 1 cut(s) 534
BspFNI CGCG 3 cut(s) 71, 168, 201
BspMAI CTGCAG 1 cut(s) 950
BspPI GGATC 2 cut(s) 489, 1498
BspT104I TTCGAA 2 cut(s) 450, 1070
BspT107I GGYRCC 5 cut(s) 95, 191, 1302, 1332, 1645
BspTNI GGTCTC 1 cut(s) 795
BsrBI CCGCTC 1 cut(s) 546
BsrDI GCAATG 2 cut(s) 1947, 2074
BsrFI RCCGGY 3 cut(s) 194, 250, 1936
BsrI ACTGG 5 cut(s) 667, 831, 894, 1455, 1857
BssAI RCCGGY 3 cut(s) 194, 250, 1936
BssMI GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1023, 1503, 2082
BssNI GRCGYC 3 cut(s) 192, 441, 1646
BssSI CACGAG 1 cut(s) 1337
BssT1I CCWWGG 4 cut(s) 352, 492, 1324, 1424
Bst2BI CACGAG 1 cut(s) 1337
Bst2UI CCWGG 3 cut(s) 400, 713, 1499
Bst4CI ACNGT 7 cut(s) 178, 384, 1186, 1250, 1401, 1618, 2056
Bst6I CTCTTC 2 cut(s) 244, 1226
BstACI GRCGYC 3 cut(s) 192, 441, 1646
BstBAI YACGTR 2 cut(s) 506, 673
BstBI TTCGAA 2 cut(s) 450, 1070
BstC8I GCNNGC 7 cut(s) 196, 233, 252, 256, 364, 1905, 1909
BstDEI CTNAG 3 cut(s) 518, 1298, 1677
BstDSI CCRYGG 3 cut(s) 492, 1424, 1614
BstF5I GGATG 5 cut(s) 219, 782, 1546, 1615, 1915
BstFNI CGCG 3 cut(s) 71, 168, 201
BstH2I RGCGCY 3 cut(s) 195, 1176, 1649
BstHHI GCGC 3 cut(s) 194, 1175, 1648
BstKTI GATC 9 cut(s) 484, 517, 538, 553, 570, 661, 1026, 1506, 2085
BstMAI GTCTC 3 cut(s) 607, 795, 1843
BstMBI GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1023, 1503, 2082
BstMCI CGRYCG 3 cut(s) 199, 570, 1626
BstMWI GCNNNNNNNGC 9 cut(s) 62, 71, 198, 1010, 1426, 1435, 1591, 1619, 2115
BstNI CCWGG 3 cut(s) 400, 713, 1499
BstPAI GACNNNNGTC 1 cut(s) 506
BstSFI CTRYAG 2 cut(s) 380, 946
BstSLI GKGCMC 1 cut(s) 1170
BstSNI TACGTA 1 cut(s) 673
BstUI CGCG 3 cut(s) 71, 168, 201
BstV1I GCAGC 5 cut(s) 65, 123, 1142, 1251, 1407
BstV2I GAAGAC 4 cut(s) 459, 867, 915, 1688
BstX2I RGATCY 1 cut(s) 481
BstYI RGATCY 1 cut(s) 481
BstZI CGGCCG 2 cut(s) 196, 1623
Bsu15I ATCGAT 1 cut(s) 534
BsuRI GGCC 7 cut(s) 74, 198, 378, 666, 1429, 1625, 1738
BsuTUI ATCGAT 1 cut(s) 534
BtgI CCRYGG 3 cut(s) 492, 1424, 1614
BtgZI GCGATG 1 cut(s) 144
BtrI CACGTC 1 cut(s) 472
BtsCI GGATG 5 cut(s) 219, 782, 1546, 1615, 1915
BtsI GCAGTG 1 cut(s) 13
BtsIMutI CAGTG 3 cut(s) 13, 78, 1397
Cac8I GCNNGC 7 cut(s) 196, 233, 252, 256, 364, 1905, 1909
CaiI CAGNNNCTG 1 cut(s) 2060
CfoI GCGC 3 cut(s) 194, 1175, 1648
Cfr10I RCCGGY 3 cut(s) 194, 250, 1936
Cfr13I GGNCC 6 cut(s) 369, 389, 664, 956, 1843, 1954
ClaI ATCGAT 1 cut(s) 534
CseI GACGC 1 cut(s) 724
CsiI ACCWGGT 2 cut(s) 398, 1497
Csp6I GTAC 6 cut(s) 574, 613, 670, 688, 897, 1033
CspCI CAANNNNNGTGG 2 cut(s) 67, 102
CviAII CATG 4 cut(s) 436, 493, 1425, 2074
CviQI GTAC 6 cut(s) 574, 613, 670, 688, 897, 1033
DdeI CTNAG 3 cut(s) 518, 1298, 1677
DinI GGCGCC 2 cut(s) 193, 1647
DpnI GATC 9 cut(s) 483, 516, 537, 552, 569, 660, 1025, 1505, 2084
DpnII GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1023, 1503, 2082
EaeI YGGCCR 3 cut(s) 72, 196, 1623
EagI CGGCCG 2 cut(s) 196, 1623
Eam1104I CTCTTC 2 cut(s) 244, 1226
EarI CTCTTC 2 cut(s) 244, 1226
EciI GGCGGA 6 cut(s) 218, 330, 337, 783, 852, 1856
Ecl136II GAGCTC 1 cut(s) 1123
EclXI CGGCCG 2 cut(s) 196, 1623
Eco105I TACGTA 1 cut(s) 673
Eco130I CCWWGG 4 cut(s) 352, 492, 1324, 1424
Eco24I GRGCYC 1 cut(s) 1125
Eco31I GGTCTC 1 cut(s) 795
Eco32I GATATC 1 cut(s) 1087
Eco47I GGWCC 5 cut(s) 369, 389, 956, 1843, 1954
Eco52I CGGCCG 2 cut(s) 196, 1623
Eco53kI GAGCTC 1 cut(s) 1123
Eco57I CTGAAG 2 cut(s) 736, 1998
Eco72I CACGTG 1 cut(s) 506
Eco88I CYCGRG 1 cut(s) 1846
EcoICRI GAGCTC 1 cut(s) 1123
EcoO109I RGGNCCY 2 cut(s) 369, 389
EcoRII CCWGG 3 cut(s) 398, 711, 1497
EcoRV GATATC 1 cut(s) 1087
EcoT14I CCWWGG 4 cut(s) 352, 492, 1324, 1424
EcoT38I GRGCYC 1 cut(s) 1125
EgeI GGCGCC 2 cut(s) 193, 1647
EheI GGCGCC 2 cut(s) 193, 1647
ErhI CCWWGG 4 cut(s) 352, 492, 1324, 1424
Esp3I CGTCTC 1 cut(s) 607
FaeI CATG 4 cut(s) 439, 496, 1428, 2077
FalI AAGNNNNNCTT 2 cut(s) 859, 891
FaqI GGGAC 5 cut(s) 355, 375, 1588, 1642, 1967
FatI CATG 4 cut(s) 435, 492, 1424, 2073
FauI CCCGC 3 cut(s) 173, 355, 359
FblI GTMKAC 4 cut(s) 528, 707, 1283, 1933
FokI GGATG 5 cut(s) 206, 769, 1553, 1622, 1902
FriOI GRGCYC 1 cut(s) 1125
FspBI CTAG 3 cut(s) 585, 882, 1052
GlaI GCGC 3 cut(s) 193, 1174, 1647
GsuI CTGGAG 2 cut(s) 1736, 1874
HaeII RGCGCY 3 cut(s) 195, 1176, 1649
HaeIII GGCC 7 cut(s) 74, 198, 378, 666, 1429, 1625, 1738
HgaI GACGC 1 cut(s) 724
HhaI GCGC 3 cut(s) 194, 1175, 1648
Hin1I GRCGYC 3 cut(s) 192, 441, 1646
Hin1II CATG 4 cut(s) 439, 496, 1428, 2077
Hin6I GCGC 3 cut(s) 192, 1173, 1646
HinP1I GCGC 3 cut(s) 192, 1173, 1646
HincII GTYRAC 5 cut(s) 529, 708, 1284, 1662, 1833
HindII GTYRAC 5 cut(s) 529, 708, 1284, 1662, 1833
HinfI GANTC 8 cut(s) 318, 344, 619, 727, 767, 820, 1067, 1158
Hpy188III TCNNGA 7 cut(s) 392, 541, 656, 1057, 1388, 1919, 2080
HpyAV CCTTC 6 cut(s) 76, 947, 1096, 1336, 1538, 2016
HpyCH4III ACNGT 7 cut(s) 178, 384, 1186, 1250, 1401, 1618, 2056
HpyCH4IV ACGT 6 cut(s) 441, 471, 505, 600, 672, 690
HpyCH4V TGCA 7 cut(s) 42, 948, 1239, 1480, 1813, 2047, 2067
HpyF10VI GCNNNNNNNGC 9 cut(s) 62, 71, 198, 1010, 1426, 1435, 1591, 1619, 2115
HpyF3I CTNAG 3 cut(s) 518, 1298, 1677
HpySE526I ACGT 6 cut(s) 441, 471, 505, 600, 672, 690
Hsp92I GRCGYC 3 cut(s) 192, 441, 1646
Hsp92II CATG 4 cut(s) 439, 496, 1428, 2077
HspAI GCGC 3 cut(s) 192, 1173, 1646
KasI GGCGCC 2 cut(s) 191, 1645
KflI GGGWCCC 2 cut(s) 369, 389
KroI GCCGGC 2 cut(s) 194, 250
KroNI GCCGGC 2 cut(s) 196, 252
Kzo9I GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1023, 1503, 2082
LmnI GCTCC 4 cut(s) 747, 962, 1120, 1326
Lsp1109I GCAGC 5 cut(s) 65, 123, 1142, 1251, 1407
LweI GCATC 3 cut(s) 121, 1362, 1600
MabI ACCWGGT 2 cut(s) 398, 1497
MaeI CTAG 3 cut(s) 585, 882, 1052
MaeII ACGT 6 cut(s) 441, 471, 505, 600, 672, 690
MaeIII GTNAC 4 cut(s) 11, 283, 979, 2077
MalI GATC 9 cut(s) 483, 516, 537, 552, 569, 660, 1025, 1505, 2084
MbiI CCGCTC 1 cut(s) 546
MboI GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1023, 1503, 2082
MfeI CAATTG 2 cut(s) 1287, 1740
MflI RGATCY 1 cut(s) 481
MhlI GDGCHC 3 cut(s) 1125, 1170, 1596
Mly113I GGCGCC 2 cut(s) 192, 1646
MlyI GAGTC 2 cut(s) 312, 338
MmeI TCCRAC 6 cut(s) 363, 384, 454, 848, 1230, 1254
MroNI GCCGGC 2 cut(s) 194, 250
MroXI GAANNNNTTC 2 cut(s) 1106, 1265
MseI TTAA 3 cut(s) 294, 1092, 1518
MslI CAYNNNNRTG 4 cut(s) 434, 815, 1094, 1818
MspA1I CMGCKG 1 cut(s) 1420
MunI CAATTG 2 cut(s) 1287, 1740
Mva1269I GAATGC 2 cut(s) 42, 2069
MvaI CCWGG 3 cut(s) 400, 713, 1499
MvnI CGCG 3 cut(s) 71, 168, 201
MwoI GCNNNNNNNGC 9 cut(s) 62, 71, 198, 1010, 1426, 1435, 1591, 1619, 2115
NaeI GCCGGC 2 cut(s) 196, 252
NarI GGCGCC 2 cut(s) 192, 1646
NciI CCSGG 7 cut(s) 638, 703, 787, 960, 1015, 1607, 1627
NcoI CCATGG 2 cut(s) 492, 1424
NdeII GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1023, 1503, 2082
NgoMIV GCCGGC 2 cut(s) 194, 250
NlaIII CATG 4 cut(s) 439, 496, 1428, 2077
NmeAIII GCCGAG 1 cut(s) 354
NmuCI GTSAC 4 cut(s) 11, 283, 979, 2077
NspV TTCGAA 2 cut(s) 450, 1070
PasI CCCWGGG 1 cut(s) 712
PcsI WCGNNNNNNNCGW 5 cut(s) 417, 447, 468, 1280, 1344
PctI GAATGC 2 cut(s) 42, 2069
PdiI GCCGGC 2 cut(s) 196, 252
PdmI GAANNNNTTC 2 cut(s) 1106, 1265
PfeI GAWTC 6 cut(s) 619, 727, 767, 820, 1067, 1158
PflMI CCANNNNNTGG 1 cut(s) 1963
PfoI TCCNGGA 1 cut(s) 958
Ple19I CGATCG 1 cut(s) 570
PleI GAGTC 2 cut(s) 312, 338
PluTI GGCGCC 2 cut(s) 195, 1649
PmaCI CACGTG 1 cut(s) 506
PmlI CACGTG 1 cut(s) 506
PpsI GAGTC 2 cut(s) 312, 338
Ppu21I YACGTR 2 cut(s) 506, 673
PpuMI RGGWCCY 2 cut(s) 369, 389
PshAI GACNNNNGTC 1 cut(s) 506
Psp124BI GAGCTC 1 cut(s) 1125
Psp5II RGGWCCY 2 cut(s) 369, 389
Psp6I CCWGG 3 cut(s) 398, 711, 1497
PspCI CACGTG 1 cut(s) 506
PspGI CCWGG 3 cut(s) 398, 711, 1497
PspPI GGNCC 6 cut(s) 369, 389, 664, 956, 1843, 1954
PspPPI RGGWCCY 2 cut(s) 369, 389
PstI CTGCAG 1 cut(s) 950
PstNI CAGNNNCTG 1 cut(s) 2060
PsuI RGATCY 1 cut(s) 481
PvuI CGATCG 1 cut(s) 570
RsaI GTAC 6 cut(s) 575, 614, 671, 689, 898, 1034
RsaNI GTAC 6 cut(s) 574, 613, 670, 688, 897, 1033
RseI CAYNNNNRTG 4 cut(s) 434, 815, 1094, 1818
SacI GAGCTC 1 cut(s) 1125
SalI GTCGAC 3 cut(s) 527, 706, 1282
SaqAI TTAA 3 cut(s) 294, 1092, 1518
Sau3AI GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1023, 1503, 2082
Sau96I GGNCC 6 cut(s) 369, 389, 664, 956, 1843, 1954
ScaI AGTACT 1 cut(s) 898
SchI GAGTC 2 cut(s) 312, 338
SduI GDGCHC 3 cut(s) 1125, 1170, 1596
SexAI ACCWGGT 2 cut(s) 398, 1497
SfaNI GCATC 3 cut(s) 121, 1362, 1600
SfcI CTRYAG 2 cut(s) 380, 946
SfoI GGCGCC 2 cut(s) 193, 1647
SfuI TTCGAA 2 cut(s) 450, 1070
SinI GGWCC 5 cut(s) 369, 389, 956, 1843, 1954
SmiMI CAYNNNNRTG 4 cut(s) 434, 815, 1094, 1818
SmlI CTYRAG 2 cut(s) 539, 1124
SmoI CTYRAG 2 cut(s) 539, 1124
SnaBI TACGTA 1 cut(s) 673
SpeI ACTAGT 1 cut(s) 1051
SspDI GGCGCC 2 cut(s) 191, 1645
SspMI CTAG 3 cut(s) 585, 882, 1052
SstI GAGCTC 1 cut(s) 1125
StyI CCWWGG 4 cut(s) 352, 492, 1324, 1424
TaaI ACNGT 7 cut(s) 178, 384, 1186, 1250, 1401, 1618, 2056
TaiI ACGT 6 cut(s) 444, 474, 508, 603, 675, 693
TatI WGTACW 2 cut(s) 896, 1032
TfiI GAWTC 6 cut(s) 619, 727, 767, 820, 1067, 1158
Tru1I TTAA 3 cut(s) 294, 1092, 1518
Tru9I TTAA 3 cut(s) 294, 1092, 1518
TscAI CASTG 3 cut(s) 20, 85, 1404
TseFI GTSAC 4 cut(s) 11, 283, 979, 2077
TseI GCWGC 5 cut(s) 53, 111, 1130, 1239, 1420
Tsp45I GTSAC 4 cut(s) 11, 283, 979, 2077
TspDTI ATGAA 3 cut(s) 833, 1380, 1557
TspGWI ACGGA 3 cut(s) 271, 514, 737
TspRI CASTG 3 cut(s) 20, 85, 1404
Van91I CCANNNNNTGG 1 cut(s) 1963
VpaK11BI GGWCC 5 cut(s) 369, 389, 956, 1843, 1954
XapI RAATTY 4 cut(s) 83, 1309, 1350, 1443
XmiI GTMKAC 4 cut(s) 528, 707, 1283, 1933
XmnI GAANNNNTTC 2 cut(s) 1106, 1265
XspI CTAG 3 cut(s) 585, 882, 1052
ZraI GACGTC 1 cut(s) 442
ZrmI AGTACT 1 cut(s) 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.