pycom04g09730

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0015958
Physical Location & Seq
Forward (+)
12464586 .. 12466733
2148 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g09730.1

Sequence Viewer

Length: 2148 bp
ATGTTGGATTTTCACTGCTTCCTTTACATTCTGATTCTCTGCATTTCCAAAGCCGCCGTGTTCGCCGTCGTCGCCGCCACCGAATTTGACTTCGGCACCTTGACTCTGAGCAGCCTGAAGCTCATTGGCGATGCCCACCTGAGCAATGGGAGTGTGCGGCTCACCCGCGACCTCGCCGTTCCCAACTCCGGCGCCGGCCGCGTTTTATACTCCAAACCCATTCGCTTCCGCCAGCCTTCCTCCCCAGTCCCGGCGAGCTTCTCCACATTCTTCTCATTCTCCGTCACCAACCTCAACCCCTCCTCCATCGGCGGCGGTCTCGCGTTCCTCATCTCCCCAGACGACGTCGCTCTCGGCGACGCCGGCGGGTTCCTCGGCCTTCAGTCGGGTCCGGATACAGGGTCGGGTTTCGTGGCCGTCGAATTCGATACGCTCATGGACGTCGAGTTCAAGGACATCAACGGGAACCACGTCGGGTTGGATCTGGACTCCATAGTGTCGACACGTGTCAGCGATCTGGATGCCGTCGACATCGATCTCAAGAGCGGCGATCTGGTGAACTCGTGGATCGAGTACGACGGGTCGAGTAAGGTCATCAACGTCTCGGTTTCGTACTCGAATCTGAAACCCAAAGACCCGGTTCTATCATTCTCCCTCGATCTAGCCCAGTACGTCAGCGATTTTATGTACGTTGGGTTTTCCGGGTCGACCCAGGGAAGCACGGAGATTCACAGCGTCGAGTGGTGGAGCTTCAGCTCGTCGTTCGATTCCCCTGTTTCGCCATCCGGGTCGGCGTCTCCGCCGCCGCCGAGCACTACTTTGATGAACCCAACTGCCAATTCAGTAAAATCTCCACCACCTTCAATGCCGCCAAGTGGGTCTTCTTCTAGTACTGCTAGCGGTACCAATCAGAAGAGCAAGTCGTCTTCTTCCTGCCATAACCAGCTCTGCAGAGAAGGTCCGGGAGCCGTCGCCGGAGTTGTCACCGCTTCCGCTTTTGTGTTGGCTGTGTTTGCGGGTGTTTTAATCTGGGTTTACTCCAAGAAAGTCAAACAAGTCAAGAAATCGGATTCGAATTTCGCTTCGGATATCATCAAAATGCCGAAGGAGTTCACTTACAGGGAGCTCAAGGAAGCAACTAAGTGCTTCAATGCCAATAGAATAATCGGGCATGGTGCTTTTGGAACTGTCTACAAAGGCATATTATCCGACACCGGTGACATTGTCGCGGTGAAGAGGTGCAGCCACAGCAGTCAGGGGAAGAACGAGTTCTTGTCGGAATTGTCGATAATTGGAACTCTTAGGCACCGGAATTTGGTGAGGCTCCAAGGCTGGTGCCACGAGAAGGGCGAAATTTTGTTGGTGTATGATTTAATGCCGAATGGCAGTCTCGACAAGGCATTGTTCGAGGCAAGAACGCCGCTTCCATGGCCGCACCGGCGAAAAATTCTATTGGGAGTTGCCTCTGCTCTGGCCTATTTGCACCAAGAATGTGAAAATCAGGTGATCCATAGGGACATTAAGACCAGTAACATAATGCTGGATGAAGGGTTCAGTGCCCGGTTGGGGGATTTCGGTTTGGCGAGGCAAATCGAGCACGATAAGTCGCCAGACGCCACGGTGGCGGCCGGGACAATGGGGTACCTGGCGCCGGAGTACTTGTTGACAGGCAGAGCTACTGAGAAAACGGATGTGTTTAGCTTTGGGGCGGTGGTGCTTGAGGTGGGGAGTGGGAGGAGGCCAATTGAGAGGGAGGTGAGTGGGGTAGGGAAAGCAGGAGCGTGTCATAATTTGGTGGAGTGGGTGTGGGCATTGCACAGAGATGAGCGGTTGTTGACGGCGGCGGACCCGAGATTGGAGGGGCAATTTGATGAGGGGGAGATGAGGAAGGTGCTGCTGGTTGGGCTGGCTTGCTCACATCCTGACCCTAATTGTAGGCCAGCAATGAGAGGGGTGGTTCAGATGCTGGTGGGTGAAGCTCAAGTCCCAATTGTGCCAAGAACAAAACCTTGTACGAGTTTCAGCACTTCACATCTTTTGTTGAGCTTGCAGGACAGTGTTTCTGACTGCAATGGCATGATCACCATCTCCACCTCCTCCTCCGAACTCAGCTTCGGCGCCGGCGCCGGCGGTGATCACATCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase S1FA

Protein Analysis

716

Amino Acids

76.61

Weight (kDa)

5.93

Isoelectric Point (pI)

36.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 27 - 258 4.2e-62 Legume lectin domain
Lectin_L-type_dom PF18483 39 - 247 5.3e-07 Legume lectin beta-barrel domain
Pkinase PF00069 384 - 597 4.6e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 385 - 654 4.3e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53380
fragaria_vesca FvH4_6g15560
malus_domestica MD04G1102000.v1.1 MD12G1121700.v1.1
prunus_persica Prupe.6G239500_v2.0.a1
pyrus_communis pycom04g09730 pycom12g12070
rosa_chinensis RchiOBHm_Chr3g0469121
rosa_laevigata RLG00000024371
rosa_multiflora Rmu_sc0000302.1_g000014 Rmu_sc0007933.1_g000020
rosa_roxburghii Rroxscaffold_6G00412190
rosa_rugosa Rorug03G0099900
rosa_samantha Rh3AG148900
rosa_wichuraiana Rw3G014050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 348, 444
Acc65I GGTACC 2 cut(s) 902, 1641
AccB1I GGYRCC 9 cut(s) 95, 191, 902, 1305, 1335, 1641, 1648, 2117, 2123
AccBSI CCGCTC 2 cut(s) 546, 1828
AccI GTMKAC 4 cut(s) 500, 528, 707, 1191
AccII CGCG 4 cut(s) 168, 201, 323, 1229
AccIII TCCGGA 1 cut(s) 391
AclWI GGATC 3 cut(s) 489, 575, 1501
AcoI YGGCCR 4 cut(s) 196, 414, 1430, 1626
AcsI RAATTY 6 cut(s) 83, 422, 1075, 1312, 1353, 1446
AcuI CTGAAG 3 cut(s) 137, 365, 736
AcvI CACGTG 1 cut(s) 506
AcyI GRCGYC 9 cut(s) 192, 345, 360, 441, 794, 1614, 1649, 2118, 2124
AfaI GTAC 9 cut(s) 575, 614, 671, 689, 892, 904, 1643, 1658, 2014
AflIII ACRYGT 2 cut(s) 503, 505
AgeI ACCGGT 1 cut(s) 1214
AgsI TTSAA 3 cut(s) 451, 864, 1150
AjiI CACGTC 1 cut(s) 472
AjnI CCWGG 2 cut(s) 711, 1644
AjuI GAANNNNNNNTTGG 4 cut(s) 823, 855, 865, 897
AloI GAACNNNNNNTCC 6 cut(s) 431, 463, 1408, 1440, 1535, 1567
Alw21I GWGCWC 3 cut(s) 815, 1128, 1599
Alw26I GTCTC 4 cut(s) 323, 607, 801, 1394
AlwI GGATC 3 cut(s) 489, 575, 1501
AlwNI CAGNNNCTG 1 cut(s) 1966
Ama87I CYCGRG 1 cut(s) 1849
Aor13HI TCCGGA 1 cut(s) 391
AoxI GGCC 8 cut(s) 196, 376, 414, 1430, 1473, 1626, 1739, 1937
ApeKI GCWGC 3 cut(s) 111, 1242, 1894
ApoI RAATTY 6 cut(s) 83, 422, 1075, 1312, 1353, 1446
AsiGI ACCGGT 1 cut(s) 1214
Asp700I GAANNNNTTC 2 cut(s) 1109, 1268
Asp718I GGTACC 2 cut(s) 902, 1641
AspLEI GCGC 4 cut(s) 194, 1651, 2120, 2126
AspS9I GGNCC 3 cut(s) 389, 959, 1846
AsuC2I CCSGG 7 cut(s) 251, 638, 703, 787, 963, 1561, 1630
AsuII TTCGAA 1 cut(s) 1073
AsuNHI GCTAGC 1 cut(s) 896
AvaI CYCGRG 1 cut(s) 1849
AvaII GGWCC 3 cut(s) 389, 959, 1846
BaeGI GKGCMC 1 cut(s) 1561
BanI GGYRCC 9 cut(s) 95, 191, 902, 1305, 1335, 1641, 1648, 2117, 2123
BanII GRGCYC 1 cut(s) 1128
BauI CACGAG 2 cut(s) 562, 1340
BbrPI CACGTG 1 cut(s) 506
BbsI GAAGAC 2 cut(s) 873, 918
Bbv12I GWGCWC 3 cut(s) 815, 1128, 1599
BbvI GCAGC 3 cut(s) 123, 1254, 1881
BccI CCATC 3 cut(s) 314, 790, 2093
BceAI ACGGC 7 cut(s) 41, 50, 161, 401, 509, 953, 1854
BcgI CGANNNNNNTGC 2 cut(s) 503, 537
BciT130I CCWGG 2 cut(s) 713, 1646
BciVI GTATCC 1 cut(s) 388
BclI TGATCA 2 cut(s) 2079, 2134
BcnI CCSGG 7 cut(s) 251, 638, 703, 787, 963, 1561, 1630
BcoDI GTCTC 4 cut(s) 323, 607, 801, 1394
BfaI CTAG 3 cut(s) 662, 888, 897
BfmI CTRYAG 1 cut(s) 949
BfoI RGCGCY 4 cut(s) 195, 1652, 2121, 2127
BfuI GTATCC 1 cut(s) 388
BglI GCCNNNNNGGC 2 cut(s) 1438, 1622
BmcAI AGTACT 2 cut(s) 892, 1658
Bme1390I CCNGG 9 cut(s) 251, 638, 703, 713, 787, 963, 1561, 1630, 1646
Bme18I GGWCC 3 cut(s) 389, 959, 1846
BmeT110I CYCGRG 1 cut(s) 1849
BmgBI CACGTC 1 cut(s) 472
BmgT120I GGNCC 3 cut(s) 389, 959, 1846
BmrFI CCNGG 9 cut(s) 251, 638, 703, 713, 787, 963, 1561, 1630, 1646
BmrI ACTGGG 2 cut(s) 239, 661
BmsI GCATC 3 cut(s) 121, 511, 1953
BmtI GCTAGC 1 cut(s) 900
BmuI ACTGGG 2 cut(s) 239, 661
BoxI GACNNNNGTC 1 cut(s) 506
BpiI GAAGAC 2 cut(s) 873, 918
Bpu10I CCTNAGC 1 cut(s) 140
Bpu14I TTCGAA 1 cut(s) 1073
BpuEI CTTGAG 4 cut(s) 524, 1112, 1739, 1965
BpuMI CCSGG 7 cut(s) 251, 638, 703, 787, 963, 1561, 1630
Bsa29I ATCGAT 1 cut(s) 534
BsaAI YACGTR 1 cut(s) 506
BsaBI GATNNNNATC 1 cut(s) 2084
BsaHI GRCGYC 9 cut(s) 192, 345, 360, 441, 794, 1614, 1649, 2118, 2124
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 6 cut(s) 373, 711, 712, 1327, 1427, 1617
BsaWI WCCGGW 3 cut(s) 391, 1214, 1308
BsaXI ACNNNNNCTCC 6 cut(s) 287, 317, 1646, 1676, 1790, 1820
Bse118I RCCGGY 6 cut(s) 194, 362, 1214, 1437, 2120, 2126
Bse1I ACTGG 3 cut(s) 245, 667, 1527
Bse3DI GCAATG 4 cut(s) 151, 1811, 1950, 2077
Bse8I GATNNNNATC 1 cut(s) 2084
BseAI TCCGGA 1 cut(s) 391
BseBI CCWGG 2 cut(s) 713, 1646
BseCI ATCGAT 1 cut(s) 534
BseDI CCNNGG 6 cut(s) 373, 711, 712, 1327, 1427, 1617
BseGI GGATG 5 cut(s) 526, 782, 1549, 1696, 1918
BseJI GATNNNNATC 1 cut(s) 2084
BseMI GCAATG 4 cut(s) 151, 1811, 1950, 2077
BseMII CTCAG 4 cut(s) 98, 131, 1671, 2122
BseNI ACTGG 3 cut(s) 245, 667, 1527
BseRI GAGGAG 4 cut(s) 292, 1750, 2086, 2089
BseSI GKGCMC 1 cut(s) 1561
BseX3I CGGCCG 2 cut(s) 196, 1626
BseXI GCAGC 3 cut(s) 123, 1254, 1881
BsgI GTGCAG 1 cut(s) 1261
Bsh1236I CGCG 4 cut(s) 168, 201, 323, 1229
Bsh1285I CGRYCG 2 cut(s) 199, 1629
BshFI GGCC 8 cut(s) 198, 378, 416, 1432, 1475, 1628, 1741, 1939
BshNI GGYRCC 9 cut(s) 95, 191, 902, 1305, 1335, 1641, 1648, 2117, 2123
BshTI ACCGGT 1 cut(s) 1214
BshVI ATCGAT 1 cut(s) 534
BsiEI CGRYCG 2 cut(s) 199, 1629
BsiHKAI GWGCWC 3 cut(s) 815, 1128, 1599
BsiHKCI CYCGRG 1 cut(s) 1849
BslFI GGGAC 4 cut(s) 233, 1529, 1645, 1970
BsmAI GTCTC 4 cut(s) 323, 607, 801, 1394
BsmBI CGTCTC 2 cut(s) 607, 801
BsmFI GGGAC 4 cut(s) 233, 1529, 1645, 1970
BsnI GGCC 8 cut(s) 198, 378, 416, 1432, 1475, 1628, 1741, 1939
Bso31I GGTCTC 1 cut(s) 323
BsoBI CYCGRG 1 cut(s) 1849
Bsp119I TTCGAA 1 cut(s) 1073
Bsp1286I GDGCHC 4 cut(s) 815, 1128, 1561, 1599
Bsp13I TCCGGA 1 cut(s) 391
Bsp143I GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1506, 2079, 2134
Bsp19I CCATGG 1 cut(s) 1427
BspANI GGCC 8 cut(s) 198, 378, 416, 1432, 1475, 1628, 1741, 1939
BspCNI CTCAG 4 cut(s) 99, 132, 1672, 2121
BspDI ATCGAT 1 cut(s) 534
BspEI TCCGGA 1 cut(s) 391
BspFNI CGCG 4 cut(s) 168, 201, 323, 1229
BspMAI CTGCAG 1 cut(s) 953
BspOI GCTAGC 1 cut(s) 900
BspPI GGATC 3 cut(s) 489, 575, 1501
BspQI GCTCTTC 1 cut(s) 908
BspT104I TTCGAA 1 cut(s) 1073
BspT107I GGYRCC 9 cut(s) 95, 191, 902, 1305, 1335, 1641, 1648, 2117, 2123
BspTNI GGTCTC 1 cut(s) 323
BsrBI CCGCTC 2 cut(s) 546, 1828
BsrDI GCAATG 4 cut(s) 151, 1811, 1950, 2077
BsrFI RCCGGY 6 cut(s) 194, 362, 1214, 1437, 2120, 2126
BsrI ACTGG 3 cut(s) 245, 667, 1527
BssAI RCCGGY 6 cut(s) 194, 362, 1214, 1437, 2120, 2126
BssECI CCNNGG 6 cut(s) 373, 711, 712, 1327, 1427, 1617
BssMI GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1506, 2079, 2134
BssNI GRCGYC 9 cut(s) 192, 345, 360, 441, 794, 1614, 1649, 2118, 2124
BssSI CACGAG 2 cut(s) 562, 1340
BssT1I CCWWGG 2 cut(s) 1327, 1427
Bst2BI CACGAG 2 cut(s) 562, 1340
Bst2UI CCWGG 2 cut(s) 713, 1646
Bst4CI ACNGT 3 cut(s) 1189, 1621, 2057
Bst6I CTCTTC 2 cut(s) 908, 1229
BstACI GRCGYC 9 cut(s) 192, 345, 360, 441, 794, 1614, 1649, 2118, 2124
BstBAI YACGTR 1 cut(s) 506
BstBI TTCGAA 1 cut(s) 1073
BstDEI CTNAG 6 cut(s) 107, 140, 1140, 1301, 1680, 2108
BstDSI CCRYGG 2 cut(s) 1427, 1617
BstF5I GGATG 5 cut(s) 526, 782, 1549, 1696, 1918
BstFNI CGCG 4 cut(s) 168, 201, 323, 1229
BstH2I RGCGCY 4 cut(s) 195, 1652, 2121, 2127
BstHHI GCGC 4 cut(s) 194, 1651, 2120, 2126
BstKTI GATC 9 cut(s) 484, 517, 538, 553, 570, 661, 1509, 2082, 2137
BstMAI GTCTC 4 cut(s) 323, 607, 801, 1394
BstMBI GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1506, 2079, 2134
BstMCI CGRYCG 2 cut(s) 199, 1629
BstNI CCWGG 2 cut(s) 713, 1646
BstPAI GACNNNNGTC 1 cut(s) 506
BstSCI CCNGG 9 cut(s) 249, 636, 701, 711, 785, 961, 1559, 1628, 1644
BstSFI CTRYAG 1 cut(s) 949
BstSLI GKGCMC 1 cut(s) 1561
BstUI CGCG 4 cut(s) 168, 201, 323, 1229
BstV1I GCAGC 3 cut(s) 123, 1254, 1881
BstV2I GAAGAC 2 cut(s) 873, 918
BstX2I RGATCY 1 cut(s) 481
BstYI RGATCY 1 cut(s) 481
BstZI CGGCCG 2 cut(s) 196, 1626
Bsu15I ATCGAT 1 cut(s) 534
BsuI GTATCC 1 cut(s) 388
BsuRI GGCC 8 cut(s) 198, 378, 416, 1432, 1475, 1628, 1741, 1939
BsuTUI ATCGAT 1 cut(s) 534
BtgI CCRYGG 2 cut(s) 1427, 1617
BtgZI GCGATG 1 cut(s) 144
BtrI CACGTC 1 cut(s) 472
BtsCI GGATG 5 cut(s) 526, 782, 1549, 1696, 1918
BtsI GCAGTG 1 cut(s) 13
BtsIMutI CAGTG 3 cut(s) 13, 1561, 2062
CaiI CAGNNNCTG 1 cut(s) 1966
CfoI GCGC 4 cut(s) 194, 1651, 2120, 2126
Cfr10I RCCGGY 6 cut(s) 194, 362, 1214, 1437, 2120, 2126
Cfr13I GGNCC 3 cut(s) 389, 959, 1846
ClaI ATCGAT 1 cut(s) 534
CseI GACGC 4 cut(s) 368, 724, 783, 1622
Csp6I GTAC 9 cut(s) 574, 613, 670, 688, 891, 903, 1642, 1657, 2013
CspAI ACCGGT 1 cut(s) 1214
CspCI CAANNNNNGTGG 2 cut(s) 67, 102
CviAII CATG 4 cut(s) 436, 1172, 1428, 2077
CviQI GTAC 9 cut(s) 574, 613, 670, 688, 891, 903, 1642, 1657, 2013
DdeI CTNAG 6 cut(s) 107, 140, 1140, 1301, 1680, 2108
DinI GGCGCC 4 cut(s) 193, 1650, 2119, 2125
DpnI GATC 9 cut(s) 483, 516, 537, 552, 569, 660, 1508, 2081, 2136
DpnII GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1506, 2079, 2134
EaeI YGGCCR 4 cut(s) 196, 414, 1430, 1626
EagI CGGCCG 2 cut(s) 196, 1626
Eam1104I CTCTTC 2 cut(s) 908, 1229
EarI CTCTTC 2 cut(s) 908, 1229
EciI GGCGGA 3 cut(s) 218, 789, 1859
Ecl136II GAGCTC 1 cut(s) 1126
EclXI CGGCCG 2 cut(s) 196, 1626
Eco130I CCWWGG 2 cut(s) 1327, 1427
Eco24I GRGCYC 1 cut(s) 1128
Eco31I GGTCTC 1 cut(s) 323
Eco32I GATATC 1 cut(s) 1090
Eco47I GGWCC 3 cut(s) 389, 959, 1846
Eco52I CGGCCG 2 cut(s) 196, 1626
Eco53kI GAGCTC 1 cut(s) 1126
Eco57I CTGAAG 3 cut(s) 137, 365, 736
Eco72I CACGTG 1 cut(s) 506
Eco88I CYCGRG 1 cut(s) 1849
EcoICRI GAGCTC 1 cut(s) 1126
EcoRI GAATTC 1 cut(s) 422
EcoRII CCWGG 2 cut(s) 711, 1644
EcoRV GATATC 1 cut(s) 1090
EcoT14I CCWWGG 2 cut(s) 1327, 1427
EcoT38I GRGCYC 1 cut(s) 1128
EgeI GGCGCC 4 cut(s) 193, 1650, 2119, 2125
EheI GGCGCC 4 cut(s) 193, 1650, 2119, 2125
ErhI CCWWGG 2 cut(s) 1327, 1427
Esp3I CGTCTC 2 cut(s) 607, 801
FaeI CATG 4 cut(s) 439, 1175, 1431, 2080
FalI AAGNNNNNCTT 2 cut(s) 865, 897
FaqI GGGAC 4 cut(s) 233, 1529, 1645, 1970
FatI CATG 4 cut(s) 435, 1171, 1427, 2076
FauI CCCGC 3 cut(s) 173, 359, 1009
FbaI TGATCA 2 cut(s) 2079, 2134
FblI GTMKAC 4 cut(s) 500, 528, 707, 1191
FokI GGATG 5 cut(s) 533, 769, 1556, 1703, 1905
FriOI GRGCYC 1 cut(s) 1128
FspBI CTAG 3 cut(s) 662, 888, 897
GlaI GCGC 4 cut(s) 193, 1650, 2119, 2125
HaeII RGCGCY 4 cut(s) 195, 1652, 2121, 2127
HaeIII GGCC 8 cut(s) 198, 378, 416, 1432, 1475, 1628, 1741, 1939
HgaI GACGC 4 cut(s) 368, 724, 783, 1622
HhaI GCGC 4 cut(s) 194, 1651, 2120, 2126
Hin1I GRCGYC 9 cut(s) 192, 345, 360, 441, 794, 1614, 1649, 2118, 2124
Hin1II CATG 4 cut(s) 439, 1175, 1431, 2080
Hin6I GCGC 4 cut(s) 192, 1649, 2118, 2124
HinP1I GCGC 4 cut(s) 192, 1649, 2118, 2124
HincII GTYRAC 5 cut(s) 501, 529, 708, 1665, 1836
HindII GTYRAC 5 cut(s) 501, 529, 708, 1665, 1836
HinfI GANTC 7 cut(s) 34, 103, 488, 619, 727, 767, 1070
Hpy166II GTNNAC 9 cut(s) 501, 529, 559, 708, 1036, 1113, 1192, 1665, 1836
Hpy188III TCNNGA 7 cut(s) 392, 485, 518, 541, 1060, 1391, 1922
Hpy8I GTNNAC 9 cut(s) 501, 529, 559, 708, 1036, 1113, 1192, 1665, 1836
HpyAV CCTTC 8 cut(s) 246, 389, 870, 950, 1099, 1339, 1541, 1882
HpyCH4III ACNGT 3 cut(s) 1189, 1621, 2057
HpyCH4IV ACGT 7 cut(s) 345, 441, 471, 505, 600, 672, 690
HpyCH4V TGCA 7 cut(s) 42, 951, 1242, 1483, 1816, 2050, 2070
HpyF3I CTNAG 6 cut(s) 107, 140, 1140, 1301, 1680, 2108
HpySE526I ACGT 7 cut(s) 345, 441, 471, 505, 600, 672, 690
Hsp92I GRCGYC 9 cut(s) 192, 345, 360, 441, 794, 1614, 1649, 2118, 2124
Hsp92II CATG 4 cut(s) 439, 1175, 1431, 2080
HspAI GCGC 4 cut(s) 192, 1649, 2118, 2124
KasI GGCGCC 4 cut(s) 191, 1648, 2117, 2123
Kpn2I TCCGGA 1 cut(s) 391
KpnI GGTACC 2 cut(s) 906, 1645
KroI GCCGGC 4 cut(s) 194, 362, 2120, 2126
KroNI GCCGGC 4 cut(s) 196, 364, 2122, 2128
Ksp22I TGATCA 2 cut(s) 2079, 2134
Kzo9I GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1506, 2079, 2134
LguI GCTCTTC 1 cut(s) 908
LmnI GCTCC 5 cut(s) 747, 965, 1123, 1329, 1778
Lsp1109I GCAGC 3 cut(s) 123, 1254, 1881
LweI GCATC 3 cut(s) 121, 511, 1953
MaeI CTAG 3 cut(s) 662, 888, 897
MaeII ACGT 7 cut(s) 345, 441, 471, 505, 600, 672, 690
MaeIII GTNAC 4 cut(s) 283, 982, 1217, 1529
MalI GATC 9 cut(s) 483, 516, 537, 552, 569, 660, 1508, 2081, 2136
MbiI CCGCTC 2 cut(s) 546, 1828
MboI GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1506, 2079, 2134
MboII GAAGA 8 cut(s) 262, 873, 876, 918, 921, 925, 1246, 1273
MfeI CAATTG 2 cut(s) 1743, 1989
MflI RGATCY 1 cut(s) 481
MhlI GDGCHC 4 cut(s) 815, 1128, 1561, 1599
Mly113I GGCGCC 4 cut(s) 192, 1649, 2118, 2124
MlyI GAGTC 2 cut(s) 97, 482
MmeI TCCRAC 3 cut(s) 459, 1233, 1257
MreI CGCCGGCG 3 cut(s) 362, 2120, 2126
MroI TCCGGA 1 cut(s) 391
MroNI GCCGGC 4 cut(s) 194, 362, 2120, 2126
MroXI GAANNNNTTC 2 cut(s) 1109, 1268
MseI TTAA 3 cut(s) 1025, 1373, 1521
MslI CAYNNNNRTG 2 cut(s) 1097, 1821
MspR9I CCNGG 9 cut(s) 251, 638, 703, 713, 787, 963, 1561, 1630, 1646
MunI CAATTG 2 cut(s) 1743, 1989
MvaI CCWGG 2 cut(s) 713, 1646
MvnI CGCG 4 cut(s) 168, 201, 323, 1229
NaeI GCCGGC 4 cut(s) 196, 364, 2122, 2128
NarI GGCGCC 4 cut(s) 192, 1649, 2118, 2124
NciI CCSGG 7 cut(s) 251, 638, 703, 787, 963, 1561, 1630
NcoI CCATGG 1 cut(s) 1427
NdeII GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1506, 2079, 2134
NgoMIV GCCGGC 4 cut(s) 194, 362, 2120, 2126
NheI GCTAGC 1 cut(s) 896
NlaIII CATG 4 cut(s) 439, 1175, 1431, 2080
NmeAIII GCCGAG 3 cut(s) 333, 354, 834
NmuCI GTSAC 3 cut(s) 283, 982, 1217
NspV TTCGAA 1 cut(s) 1073
PasI CCCWGGG 1 cut(s) 712
PciSI GCTCTTC 1 cut(s) 908
PcsI WCGNNNNNNNCGW 6 cut(s) 78, 354, 417, 468, 1283, 1347
PdiI GCCGGC 4 cut(s) 196, 364, 2122, 2128
PdmI GAANNNNTTC 2 cut(s) 1109, 1268
PfeI GAWTC 5 cut(s) 34, 619, 727, 767, 1070
PflFI GACNNNGTC 2 cut(s) 344, 1223
PfoI TCCNGGA 1 cut(s) 961
PinAI ACCGGT 1 cut(s) 1214
PleI GAGTC 2 cut(s) 97, 482
PluTI GGCGCC 4 cut(s) 195, 1652, 2121, 2127
PmaCI CACGTG 1 cut(s) 506
PmlI CACGTG 1 cut(s) 506
PpsI GAGTC 2 cut(s) 97, 482
Ppu21I YACGTR 1 cut(s) 506
PshAI GACNNNNGTC 1 cut(s) 506
Psp124BI GAGCTC 1 cut(s) 1128
Psp6I CCWGG 2 cut(s) 711, 1644
PspCI CACGTG 1 cut(s) 506
PspGI CCWGG 2 cut(s) 711, 1644
PspPI GGNCC 3 cut(s) 389, 959, 1846
PstI CTGCAG 1 cut(s) 953
PstNI CAGNNNCTG 1 cut(s) 1966
PsuI RGATCY 1 cut(s) 481
PsyI GACNNNGTC 2 cut(s) 344, 1223
RsaI GTAC 9 cut(s) 575, 614, 671, 689, 892, 904, 1643, 1658, 2014
RsaNI GTAC 9 cut(s) 574, 613, 670, 688, 891, 903, 1642, 1657, 2013
RseI CAYNNNNRTG 2 cut(s) 1097, 1821
SacI GAGCTC 1 cut(s) 1128
SalI GTCGAC 3 cut(s) 499, 527, 706
SapI GCTCTTC 1 cut(s) 908
SaqAI TTAA 3 cut(s) 1025, 1373, 1521
Sau3AI GATC 9 cut(s) 481, 514, 535, 550, 567, 658, 1506, 2079, 2134
Sau96I GGNCC 3 cut(s) 389, 959, 1846
ScaI AGTACT 2 cut(s) 892, 1658
SchI GAGTC 2 cut(s) 97, 482
ScrFI CCNGG 9 cut(s) 251, 638, 703, 713, 787, 963, 1561, 1630, 1646
SduI GDGCHC 4 cut(s) 815, 1128, 1561, 1599
SfaNI GCATC 3 cut(s) 121, 511, 1953
SfcI CTRYAG 1 cut(s) 949
SfoI GGCGCC 4 cut(s) 193, 1650, 2119, 2125
SfuI TTCGAA 1 cut(s) 1073
SgrAI CRCCGGYG 5 cut(s) 362, 1214, 1437, 2120, 2126
SinI GGWCC 3 cut(s) 389, 959, 1846
SmiMI CAYNNNNRTG 2 cut(s) 1097, 1821
SmlI CTYRAG 4 cut(s) 539, 1127, 1718, 1980
SmoI CTYRAG 4 cut(s) 539, 1127, 1718, 1980
SspDI GGCGCC 4 cut(s) 191, 1648, 2117, 2123
SspMI CTAG 3 cut(s) 662, 888, 897
SstI GAGCTC 1 cut(s) 1128
StyD4I CCNGG 9 cut(s) 249, 636, 701, 711, 785, 961, 1559, 1628, 1644
StyI CCWWGG 2 cut(s) 1327, 1427
TaaI ACNGT 3 cut(s) 1189, 1621, 2057
TaiI ACGT 7 cut(s) 348, 444, 474, 508, 603, 675, 693
TatI WGTACW 2 cut(s) 890, 1656
TfiI GAWTC 5 cut(s) 34, 619, 727, 767, 1070
Tru1I TTAA 3 cut(s) 1025, 1373, 1521
Tru9I TTAA 3 cut(s) 1025, 1373, 1521
TscAI CASTG 3 cut(s) 20, 1561, 2062
TseFI GTSAC 3 cut(s) 283, 982, 1217
TseI GCWGC 3 cut(s) 111, 1242, 1894
Tsp45I GTSAC 3 cut(s) 283, 982, 1217
TspDTI ATGAA 2 cut(s) 839, 1560
TspGWI ACGGA 3 cut(s) 271, 737, 1703
TspRI CASTG 3 cut(s) 20, 1561, 2062
Tth111I GACNNNGTC 2 cut(s) 344, 1223
VpaK11BI GGWCC 3 cut(s) 389, 959, 1846
XapI RAATTY 6 cut(s) 83, 422, 1075, 1312, 1353, 1446
XcmI CCANNNNNNNNNTGG 1 cut(s) 143
XmiI GTMKAC 4 cut(s) 500, 528, 707, 1191
XmnI GAANNNNTTC 2 cut(s) 1109, 1268
XspI CTAG 3 cut(s) 662, 888, 897
ZraI GACGTC 2 cut(s) 346, 442
ZrmI AGTACT 2 cut(s) 892, 1658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.