Rroxscaffold_6G00412190

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0015958
Physical Location & Seq
Forward (+)
34834523 .. 34836685
2163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00412190.1

Sequence Viewer

Length: 2163 bp
ATGTCCCAACGGATTCGCTCCATCATCTTTACTACTGTAGTACTTCACCTTTACCTTTGCTTTTCCAACTTCCTCATTACGTCAGCCGTAACCGAATTCGACTTCGGAACCCTCACCCTCAGCAGCCTCAAGCTCCTCGGCGACGCCCACTTGAACAATGGCAGCGTGCGTCTCACTCGCGACCTTGCCGTCCCCACCTCCGGCGCCGGGAGAGTTCTGTACTCGAAGCCCGTCCGCTTCAAGCAACCCTCCACTCCGTTTCCGGCTAGCTTCTCCACATTCTTCACGTTCTCCGTCAGCAATTTGAACCCCTCCTCGATCGGCGGCGGGCTGGCTTTTATCATCTCGCCGGACGATGAGGTTGTCGGAGACGCCGGCGGGTTCTTGGGGCTCCAGAGCATGGAGGACCCGGGTTCGGGTTCCGGGTCGAGCTTCGTGGCGGTGGAGTTTGACACGCTCATGGACGTGGAGTTCAAGGACATTAATAGCAACCACGTGGGCCTGGATCTCAACTCCATGGTTTCCTCACAAGTCGGCGATCTTGGCGCTGCGGATATTGATCTCAAGAGCGGGGACCTCGTGAATTGCTGGATCGAATTCGGCGGGTCGAGTCGGGTCTTCAACATATCCGTTTCGTATTCGAATCTCAAACCCAGAGACCCGCTCCTCTCCTTCCCTCTCGATCTTGATCAATACGTCAACGACTTCATGTACGTCGGGTTTTCCGGGTCGACCCAGGGAAGCACCGAAATCCACAGCGTTGACTGGTGGAGCTTCAGCTCGTCTTTTGATTCAACTCCCTCAACCGGCTCAGCAGCACCGCCGCCTCCGACGACTACTCTGATGAACCCAACAGCCAACTCAGTCAAATCTCCACCGCCTTCTGTGCCTCCAACTGGGTCGGCTTCAGCTTCTAGTACTAGTACTACGCAGAAGAACAGTAAGTCTTCTTGCCATAACCAGCTTTGCAAGCAAGGTCCGGGAGCTGTTGCTGGTGTGGTCACAGCTGGGGCTTTCGTTTTGGCTCTGTTTGCCGGTGTTTTAGTTTGGGTTTACAACAAGAAGATTAGACATGTTAAGAAATCGGAGTCTAGTTTTGCTTCGGATGTGATCAAAATGCCTAAGGAGTTTAGTTATAGGGAGCTCAAGGCAGCTACGAAGTGCTTCAATGCGAATCGGGTTATTGGGCATGGTGCATTTGGGATTGTTTACAAGGGAATATTATCAGAGACGGGTGACATTGTTGCAGTGAAGAGATGTAGTCATAGTACTCAGGGGAAGAACGAGTTTTTGTCCGAGTTGTCTATTATCGGAACTCTTAGGCATCGAAATCTTGTTAGGCTTCAAGGTTGGTGCCATGAGAAGGGTGAGATTTTGTTAGTCTATGACTTGATGCCTAATGGTAGTCTTGACAAGGCATTGTTTGAGGCTAGAATGCCATTGCCGTGGCCTCATAGGCGAAAGATTCTATTAGGGGTTGCTTCGGCATTGGCCTATTTGCATCAGGAATGTGAGAATCAGGTGATCCATAGGGATGTTAAGGCCAGCAACATTATGCTGGATGAAGGGTTCAATGCGCGGTTAGGGGATTTCGGGTTGGCTAGGCAAATTGAGCATGACAAGTCCCCGGATGCCACGGCAGCGGCTGGGACAATGGGGTATTTGGCGCCAGAGTACTTGTTAACGGGCAGAGCTACTGAAAAAACGGATGTGTTTAGTTATGGAGCAGTGGTACTTGAGGTGGCTAGTGGGAGGAGGCCAATTGAGAAGGAAGTCAGTGGAGTAGGGAAAACGGCTGCTTGTAGCAATCTGGTTGAGTGGGTTTGGAGCTTGCACAGAGATGAAAGGCTGTTAACGGCAGCTGATCCAAGGTTAGAGGGTCAATTCGACGAGGCTGAAATGAGGAAAGTGCTACTAGTTGGACTCGCTTGCTCTCAGCCTGACCCAAGTGTTAGACCTACAATGAGAGCTGTGGTGCAAATGCTAGTGGGTGAAGCCGAAGTCCCAATTGTCCCAAAAACAAAACCCACCGCAACTTTTAGCACTTCACATCTCTTGTTGAGCTTGCAGGACAGTGTTTCCGATTGCAATGGCATGATCACCATCTCCACCTCCTCCTCCGAAAACAGCTTCAACGGTCTCGGTGATGATCATATCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase

Protein Analysis

720

Amino Acids

77.57

Weight (kDa)

5.96

Isoelectric Point (pI)

37.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 32 - 268 2.9e-61 Legume lectin domain
Pkinase PF00069 390 - 660 2.9e-50 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 391 - 662 1.7e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53380
fragaria_vesca FvH4_6g15560
malus_domestica MD04G1102000.v1.1 MD12G1121700.v1.1
prunus_persica Prupe.6G239500_v2.0.a1
pyrus_communis pycom04g09730 pycom12g12070
rosa_chinensis RchiOBHm_Chr3g0469121
rosa_laevigata RLG00000024371
rosa_multiflora Rmu_sc0000302.1_g000014 Rmu_sc0007933.1_g000020
rosa_roxburghii Rroxscaffold_6G00412190
rosa_rugosa Rorug03G0099900
rosa_samantha Rh3AG148900
rosa_wichuraiana Rw3G014050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 188
AccB1I GGYRCC 3 cut(s) 203, 1353, 1666
AccB7I CCANNNNNTGG 1 cut(s) 400
AccBSI CCGCTC 2 cut(s) 570, 664
AccI GTMKAC 1 cut(s) 731
AccII CGCG 2 cut(s) 180, 1579
AclWI GGATC 4 cut(s) 513, 599, 1519, 1859
AcsI RAATTY 2 cut(s) 95, 596
AcuI CTGAAG 2 cut(s) 760, 891
AcvI CACGTG 1 cut(s) 496
AcyI GRCGYC 4 cut(s) 144, 204, 372, 1667
AfaI GTAC 8 cut(s) 42, 221, 713, 919, 925, 1270, 1676, 1734
AfiI CCNNNNNNNGG 8 cut(s) 200, 207, 400, 415, 416, 806, 896, 1363
AflIII ACRYGT 1 cut(s) 1072
AhlI ACTAGT 2 cut(s) 920, 1915
AjiI CACGTC 1 cut(s) 466
AjnI CCWGG 2 cut(s) 501, 735
AloI GAACNNNNNNTCC 4 cut(s) 455, 487, 1553, 1585
Alw21I GWGCWC 1 cut(s) 1146
Alw26I GTCTC 5 cut(s) 176, 363, 651, 1223, 2144
AlwI GGATC 4 cut(s) 513, 599, 1519, 1859
AlwNI CAGNNNCTG 1 cut(s) 1646
Ama87I CYCGRG 1 cut(s) 409
AoxI GGCC 5 cut(s) 499, 1448, 1491, 1542, 1757
ApeKI GCWGC 8 cut(s) 123, 162, 548, 815, 1151, 1640, 1796, 1859
ApoI RAATTY 2 cut(s) 95, 596
AseI ATTAAT 1 cut(s) 483
Asp700I GAANNNNTTC 1 cut(s) 1163
AspLEI GCGC 4 cut(s) 206, 548, 1579, 1669
AspS9I GGNCC 4 cut(s) 406, 499, 574, 977
AsuC2I CCSGG 7 cut(s) 208, 410, 411, 424, 727, 981, 1628
AsuHPI GGTGA 8 cut(s) 38, 106, 1247, 1379, 1534, 2003, 2092, 2156
AsuII TTCGAA 1 cut(s) 641
AsuNHI GCTAGC 1 cut(s) 266
AvaI CYCGRG 1 cut(s) 409
AvaII GGWCC 3 cut(s) 406, 574, 977
AxyI CCTNAGG 1 cut(s) 1122
BanI GGYRCC 3 cut(s) 203, 1353, 1666
BanII GRGCYC 2 cut(s) 393, 1146
BauI CACGAG 1 cut(s) 578
BbrPI CACGTG 1 cut(s) 496
BbsI GAAGAC 2 cut(s) 610, 939
Bbv12I GWGCWC 1 cut(s) 1146
BbvCI CCTCAGC 1 cut(s) 119
BbvI GCAGC 8 cut(s) 135, 174, 535, 827, 1163, 1652, 1783, 1871
BccI CCATC 2 cut(s) 29, 2111
BceAI ACGGC 6 cut(s) 71, 173, 1429, 1653, 1809, 1872
BciT130I CCWGG 2 cut(s) 503, 737
BclI TGATCA 4 cut(s) 688, 1110, 2097, 2149
BcnI CCSGG 7 cut(s) 208, 410, 411, 424, 727, 981, 1628
BcoDI GTCTC 5 cut(s) 176, 363, 651, 1223, 2144
BcuI ACTAGT 2 cut(s) 920, 1915
BfaI CTAG 9 cut(s) 267, 915, 921, 1092, 1431, 1602, 1746, 1916, 1985
BfmI CTRYAG 1 cut(s) 36
BfoI RGCGCY 3 cut(s) 207, 549, 1670
BglI GCCNNNNNGGC 1 cut(s) 1456
BlpI GCTNAGC 1 cut(s) 811
BmcAI AGTACT 5 cut(s) 42, 919, 925, 1270, 1676
Bme1390I CCNGG 9 cut(s) 208, 410, 411, 424, 503, 727, 737, 981, 1628
Bme18I GGWCC 3 cut(s) 406, 574, 977
BmeT110I CYCGRG 1 cut(s) 409
BmgBI CACGTC 1 cut(s) 466
BmgT120I GGNCC 4 cut(s) 406, 499, 574, 977
BmiI GGNNCC 8 cut(s) 109, 205, 392, 408, 421, 575, 1355, 1668
BmrFI CCNGG 9 cut(s) 208, 410, 411, 424, 503, 727, 737, 981, 1628
BmrI ACTGGG 1 cut(s) 906
BmsI GCATC 4 cut(s) 1333, 1383, 1510, 1621
BmtI GCTAGC 1 cut(s) 270
BmuI ACTGGG 1 cut(s) 906
BpiI GAAGAC 2 cut(s) 610, 939
BplI GAGNNNNNCTC 2 cut(s) 648, 680
BpmI CTGGAG 1 cut(s) 377
Bpu10I CCTNAGC 1 cut(s) 119
Bpu1102I GCTNAGC 1 cut(s) 811
Bpu14I TTCGAA 1 cut(s) 641
BpuEI CTTGAG 4 cut(s) 113, 548, 1130, 1757
BpuMI CCSGG 7 cut(s) 208, 410, 411, 424, 727, 981, 1628
BsaAI YACGTR 1 cut(s) 496
BsaBI GATNNNNATC 3 cut(s) 558, 687, 2102
BsaHI GRCGYC 4 cut(s) 144, 204, 372, 1667
BsaI GGTCTC 2 cut(s) 651, 2144
BsaJI CCNNGG 9 cut(s) 136, 409, 516, 735, 736, 1445, 1626, 1635, 1868
BsaXI ACNNNNNCTCC 2 cut(s) 1716, 1746
Bsc4I CCNNNNNNNGG 8 cut(s) 200, 207, 400, 415, 416, 806, 896, 1363
Bse118I RCCGGY 3 cut(s) 374, 806, 1034
Bse1I ACTGG 2 cut(s) 770, 901
Bse21I CCTNAGG 1 cut(s) 1122
Bse3DI GCAATG 2 cut(s) 1439, 2095
Bse8I GATNNNNATC 3 cut(s) 558, 687, 2102
BseBI CCWGG 2 cut(s) 503, 737
BseDI CCNNGG 9 cut(s) 136, 409, 516, 735, 736, 1445, 1626, 1635, 1868
BseGI GGATG 5 cut(s) 1111, 1540, 1567, 1636, 1714
BseJI GATNNNNATC 3 cut(s) 558, 687, 2102
BseLI CCNNNNNNNGG 8 cut(s) 200, 207, 400, 415, 416, 806, 896, 1363
BseMI GCAATG 2 cut(s) 1439, 2095
BseMII CTCAG 5 cut(s) 133, 825, 876, 1286, 1949
BseNI ACTGG 2 cut(s) 770, 901
BseRI GAGGAG 6 cut(s) 125, 304, 656, 1768, 2104, 2107
BseXI GCAGC 8 cut(s) 135, 174, 535, 827, 1163, 1652, 1783, 1871
BseYI CCCAGC 2 cut(s) 1007, 1646
Bsh1236I CGCG 2 cut(s) 180, 1579
Bsh1285I CGRYCG 1 cut(s) 321
BshFI GGCC 5 cut(s) 501, 1450, 1493, 1544, 1759
BshNI GGYRCC 3 cut(s) 203, 1353, 1666
BsiEI CGRYCG 1 cut(s) 321
BsiHKAI GWGCWC 1 cut(s) 1146
BsiHKCI CYCGRG 1 cut(s) 409
BslFI GGGAC 6 cut(s) 176, 587, 1609, 1663, 1988, 1997
BslI CCNNNNNNNGG 8 cut(s) 200, 207, 400, 415, 416, 806, 896, 1363
BsmAI GTCTC 5 cut(s) 176, 363, 651, 1223, 2144
BsmBI CGTCTC 3 cut(s) 176, 363, 1223
BsmFI GGGAC 6 cut(s) 176, 587, 1609, 1663, 1988, 1997
BsmI GAATGC 1 cut(s) 1440
BsnI GGCC 5 cut(s) 501, 1450, 1493, 1544, 1759
Bso31I GGTCTC 2 cut(s) 651, 2144
BsoBI CYCGRG 1 cut(s) 409
Bsp119I TTCGAA 1 cut(s) 641
Bsp1286I GDGCHC 2 cut(s) 393, 1146
Bsp1720I GCTNAGC 1 cut(s) 811
Bsp19I CCATGG 1 cut(s) 516
Bsp68I TCGCGA 1 cut(s) 180
BspANI GGCC 5 cut(s) 501, 1450, 1493, 1544, 1759
BspCNI CTCAG 5 cut(s) 132, 824, 875, 1285, 1948
BspFNI CGCG 2 cut(s) 180, 1579
BspLI GGNNCC 8 cut(s) 109, 205, 392, 408, 421, 575, 1355, 1668
BspOI GCTAGC 1 cut(s) 270
BspPI GGATC 4 cut(s) 513, 599, 1519, 1859
BspT104I TTCGAA 1 cut(s) 641
BspT107I GGYRCC 3 cut(s) 203, 1353, 1666
BspTNI GGTCTC 2 cut(s) 651, 2144
BsrBI CCGCTC 2 cut(s) 570, 664
BsrDI GCAATG 2 cut(s) 1439, 2095
BsrFI RCCGGY 3 cut(s) 374, 806, 1034
BsrI ACTGG 2 cut(s) 770, 901
BssAI RCCGGY 3 cut(s) 374, 806, 1034
BssECI CCNNGG 9 cut(s) 136, 409, 516, 735, 736, 1445, 1626, 1635, 1868
BssNI GRCGYC 4 cut(s) 144, 204, 372, 1667
BssSI CACGAG 1 cut(s) 578
BssT1I CCWWGG 2 cut(s) 516, 1868
Bst2BI CACGAG 1 cut(s) 578
Bst2UI CCWGG 2 cut(s) 503, 737
Bst4CI ACNGT 4 cut(s) 37, 941, 2075, 2138
Bst6I CTCTTC 1 cut(s) 1247
BstACI GRCGYC 4 cut(s) 144, 204, 372, 1667
BstBAI YACGTR 1 cut(s) 496
BstBI TTCGAA 1 cut(s) 641
BstDEI CTNAG 7 cut(s) 119, 811, 862, 1122, 1272, 1319, 1935
BstDSI CCRYGG 3 cut(s) 516, 1445, 1635
BstF5I GGATG 5 cut(s) 1111, 1540, 1567, 1636, 1714
BstFNI CGCG 2 cut(s) 180, 1579
BstH2I RGCGCY 3 cut(s) 207, 549, 1670
BstHHI GCGC 4 cut(s) 206, 548, 1579, 1669
BstMAI GTCTC 5 cut(s) 176, 363, 651, 1223, 2144
BstMCI CGRYCG 1 cut(s) 321
BstMWI GCNNNNNNNGC 7 cut(s) 543, 886, 970, 1031, 1456, 1612, 1640
BstNI CCWGG 2 cut(s) 503, 737
BstNSI RCATGY 1 cut(s) 1076
BstSCI CCNGG 9 cut(s) 206, 408, 409, 422, 501, 725, 735, 979, 1626
BstSFI CTRYAG 1 cut(s) 36
BstUI CGCG 2 cut(s) 180, 1579
BstV1I GCAGC 8 cut(s) 135, 174, 535, 827, 1163, 1652, 1783, 1871
BstV2I GAAGAC 2 cut(s) 610, 939
BstX2I RGATCY 1 cut(s) 505
BstXI CCANNNNNNTGG 1 cut(s) 1446
BstYI RGATCY 1 cut(s) 505
Bsu36I CCTNAGG 1 cut(s) 1122
BsuRI GGCC 5 cut(s) 501, 1450, 1493, 1544, 1759
BtgI CCRYGG 3 cut(s) 516, 1445, 1635
BtrI CACGTC 1 cut(s) 466
BtsCI GGATG 5 cut(s) 1111, 1540, 1567, 1636, 1714
BtsI GCAGTG 2 cut(s) 1254, 1734
BtsIMutI CAGTG 4 cut(s) 1254, 1734, 1783, 2080
BtuMI TCGCGA 1 cut(s) 180
CaiI CAGNNNCTG 1 cut(s) 1646
CfoI GCGC 4 cut(s) 206, 548, 1579, 1669
Cfr10I RCCGGY 3 cut(s) 374, 806, 1034
Cfr13I GGNCC 4 cut(s) 406, 499, 574, 977
Cfr9I CCCGGG 1 cut(s) 409
CseI GACGC 3 cut(s) 152, 158, 380
Csp6I GTAC 8 cut(s) 41, 220, 712, 918, 924, 1269, 1675, 1733
CviAII CATG 9 cut(s) 400, 460, 517, 709, 1073, 1190, 1358, 1616, 2095
CviQI GTAC 8 cut(s) 41, 220, 712, 918, 924, 1269, 1675, 1733
DdeI CTNAG 7 cut(s) 119, 811, 862, 1122, 1272, 1319, 1935
DinI GGCGCC 2 cut(s) 205, 1668
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
Eam1104I CTCTTC 1 cut(s) 1247
EarI CTCTTC 1 cut(s) 1247
Ecl136II GAGCTC 1 cut(s) 1144
Eco130I CCWWGG 2 cut(s) 516, 1868
Eco24I GRGCYC 2 cut(s) 393, 1146
Eco31I GGTCTC 2 cut(s) 651, 2144
Eco47I GGWCC 3 cut(s) 406, 574, 977
Eco53kI GAGCTC 1 cut(s) 1144
Eco57I CTGAAG 2 cut(s) 760, 891
Eco72I CACGTG 1 cut(s) 496
Eco81I CCTNAGG 1 cut(s) 1122
Eco88I CYCGRG 1 cut(s) 409
EcoICRI GAGCTC 1 cut(s) 1144
EcoO109I RGGNCCY 2 cut(s) 406, 574
EcoRI GAATTC 2 cut(s) 95, 596
EcoRII CCWGG 2 cut(s) 501, 735
EcoT14I CCWWGG 2 cut(s) 516, 1868
EcoT38I GRGCYC 2 cut(s) 393, 1146
EgeI GGCGCC 2 cut(s) 205, 1668
EheI GGCGCC 2 cut(s) 205, 1668
ErhI CCWWGG 2 cut(s) 516, 1868
Esp3I CGTCTC 3 cut(s) 176, 363, 1223
FaeI CATG 9 cut(s) 403, 463, 520, 712, 1076, 1193, 1361, 1619, 2098
FaqI GGGAC 6 cut(s) 176, 587, 1609, 1663, 1988, 1997
FatI CATG 9 cut(s) 399, 459, 516, 708, 1072, 1189, 1357, 1615, 2094
FauI CCCGC 5 cut(s) 320, 371, 563, 596, 669
FbaI TGATCA 4 cut(s) 688, 1110, 2097, 2149
FblI GTMKAC 1 cut(s) 731
FokI GGATG 5 cut(s) 1118, 1547, 1574, 1643, 1721
FriOI GRGCYC 2 cut(s) 393, 1146
FspBI CTAG 9 cut(s) 267, 915, 921, 1092, 1431, 1602, 1746, 1916, 1985
GlaI GCGC 4 cut(s) 205, 547, 1578, 1668
GsaI CCCAGC 2 cut(s) 1011, 1650
GsuI CTGGAG 1 cut(s) 377
HaeII RGCGCY 3 cut(s) 207, 549, 1670
HaeIII GGCC 5 cut(s) 501, 1450, 1493, 1544, 1759
HgaI GACGC 3 cut(s) 152, 158, 380
HhaI GCGC 4 cut(s) 206, 548, 1579, 1669
Hin1I GRCGYC 4 cut(s) 144, 204, 372, 1667
Hin1II CATG 9 cut(s) 403, 463, 520, 712, 1076, 1193, 1361, 1619, 2098
Hin6I GCGC 4 cut(s) 204, 546, 1577, 1667
HinP1I GCGC 4 cut(s) 204, 546, 1577, 1667
HincII GTYRAC 5 cut(s) 700, 732, 763, 1683, 1854
HindII GTYRAC 5 cut(s) 700, 732, 763, 1683, 1854
HinfI GANTC 9 cut(s) 13, 610, 643, 791, 1088, 1174, 1465, 1516, 1923
HpaI GTTAAC 2 cut(s) 1683, 1854
HphI GGTGA 8 cut(s) 38, 106, 1247, 1379, 1534, 2003, 2092, 2156
Hpy166II GTNNAC 7 cut(s) 700, 732, 763, 1054, 1210, 1683, 1854
Hpy188III TCNNGA 8 cut(s) 179, 394, 565, 580, 680, 686, 1409, 1505
Hpy8I GTNNAC 7 cut(s) 700, 732, 763, 1054, 1210, 1683, 1854
Hpy99I CGWCG 4 cut(s) 146, 719, 835, 1892
HpyAV CCTTC 5 cut(s) 682, 891, 1357, 1559, 1762
HpyCH4III ACNGT 4 cut(s) 37, 941, 2075, 2138
HpyCH4IV ACGT 6 cut(s) 80, 287, 465, 495, 696, 714
HpyCH4V TGCA 8 cut(s) 969, 1196, 1247, 1501, 1834, 1978, 2068, 2088
HpyF10VI GCNNNNNNNGC 7 cut(s) 543, 886, 970, 1031, 1456, 1612, 1640
HpyF3I CTNAG 7 cut(s) 119, 811, 862, 1122, 1272, 1319, 1935
HpySE526I ACGT 6 cut(s) 80, 287, 465, 495, 696, 714
Hsp92I GRCGYC 4 cut(s) 144, 204, 372, 1667
Hsp92II CATG 9 cut(s) 403, 463, 520, 712, 1076, 1193, 1361, 1619, 2098
HspAI GCGC 4 cut(s) 204, 546, 1577, 1667
KasI GGCGCC 2 cut(s) 203, 1666
KroI GCCGGC 1 cut(s) 374
KroNI GCCGGC 1 cut(s) 376
Ksp22I TGATCA 4 cut(s) 688, 1110, 2097, 2149
KspAI GTTAAC 2 cut(s) 1683, 1854
LmnI GCTCC 9 cut(s) 23, 138, 396, 669, 771, 983, 1141, 1724, 1827
Lsp1109I GCAGC 8 cut(s) 135, 174, 535, 827, 1163, 1652, 1783, 1871
LweI GCATC 4 cut(s) 1333, 1383, 1510, 1621
MaeI CTAG 9 cut(s) 267, 915, 921, 1092, 1431, 1602, 1746, 1916, 1985
MaeII ACGT 6 cut(s) 80, 287, 465, 495, 696, 714
MaeIII GTNAC 3 cut(s) 88, 1000, 1235
MbiI CCGCTC 2 cut(s) 570, 664
MboII GAAGA 7 cut(s) 274, 610, 939, 946, 1075, 1264, 1291
MfeI CAATTG 2 cut(s) 1761, 2007
MflI RGATCY 1 cut(s) 505
MhlI GDGCHC 2 cut(s) 393, 1146
MluCI AATT 8 cut(s) 95, 301, 583, 596, 1608, 1761, 1883, 2007
Mly113I GGCGCC 2 cut(s) 204, 1667
MlyI GAGTC 3 cut(s) 619, 1097, 1917
MmeI TCCRAC 5 cut(s) 90, 346, 854, 917, 1900
MreI CGCCGGCG 1 cut(s) 374
MroNI GCCGGC 1 cut(s) 374
MroXI GAANNNNTTC 1 cut(s) 1163
MseI TTAA 5 cut(s) 483, 1077, 1539, 1682, 1853
MslI CAYNNNNRTG 5 cut(s) 458, 464, 1444, 1533, 1839
MspA1I CMGCKG 3 cut(s) 1007, 1643, 1862
MspR9I CCNGG 9 cut(s) 208, 410, 411, 424, 503, 727, 737, 981, 1628
MunI CAATTG 2 cut(s) 1761, 2007
Mva1269I GAATGC 1 cut(s) 1440
MvaI CCWGG 2 cut(s) 503, 737
MvnI CGCG 2 cut(s) 180, 1579
MwoI GCNNNNNNNGC 7 cut(s) 543, 886, 970, 1031, 1456, 1612, 1640
NaeI GCCGGC 1 cut(s) 376
NarI GGCGCC 2 cut(s) 204, 1667
NciI CCSGG 7 cut(s) 208, 410, 411, 424, 727, 981, 1628
NcoI CCATGG 1 cut(s) 516
NgoMIV GCCGGC 1 cut(s) 374
NheI GCTAGC 1 cut(s) 266
NlaIII CATG 9 cut(s) 403, 463, 520, 712, 1076, 1193, 1361, 1619, 2098
NlaIV GGNNCC 8 cut(s) 109, 205, 392, 408, 421, 575, 1355, 1668
NmeAIII GCCGAG 1 cut(s) 117
NmuCI GTSAC 2 cut(s) 1000, 1235
NruI TCGCGA 1 cut(s) 180
NspI RCATGY 1 cut(s) 1076
NspV TTCGAA 1 cut(s) 641
PasI CCCWGGG 1 cut(s) 736
PciI ACATGT 1 cut(s) 1072
PctI GAATGC 1 cut(s) 1440
PdiI GCCGGC 1 cut(s) 376
PdmI GAANNNNTTC 1 cut(s) 1163
PfeI GAWTC 6 cut(s) 13, 643, 791, 1174, 1465, 1516
PflMI CCANNNNNTGG 1 cut(s) 400
PfoI TCCNGGA 1 cut(s) 979
Ple19I CGATCG 1 cut(s) 321
PleI GAGTC 3 cut(s) 618, 1096, 1917
PluTI GGCGCC 2 cut(s) 207, 1670
PmaCI CACGTG 1 cut(s) 496
PmlI CACGTG 1 cut(s) 496
PpsI GAGTC 3 cut(s) 618, 1096, 1917
Ppu21I YACGTR 1 cut(s) 496
PpuMI RGGWCCY 2 cut(s) 406, 574
PscI ACATGT 1 cut(s) 1072
PshBI ATTAAT 1 cut(s) 483
Psp124BI GAGCTC 1 cut(s) 1146
Psp5II RGGWCCY 2 cut(s) 406, 574
Psp6I CCWGG 2 cut(s) 501, 735
PspCI CACGTG 1 cut(s) 496
PspFI CCCAGC 2 cut(s) 1007, 1646
PspGI CCWGG 2 cut(s) 501, 735
PspN4I GGNNCC 8 cut(s) 109, 205, 392, 408, 421, 575, 1355, 1668
PspPI GGNCC 4 cut(s) 406, 499, 574, 977
PspPPI RGGWCCY 2 cut(s) 406, 574
PstNI CAGNNNCTG 1 cut(s) 1646
PsuI RGATCY 1 cut(s) 505
PvuI CGATCG 1 cut(s) 321
PvuII CAGCTG 2 cut(s) 1007, 1862
RruI TCGCGA 1 cut(s) 180
RsaI GTAC 8 cut(s) 42, 221, 713, 919, 925, 1270, 1676, 1734
RsaNI GTAC 8 cut(s) 41, 220, 712, 918, 924, 1269, 1675, 1733
RseI CAYNNNNRTG 5 cut(s) 458, 464, 1444, 1533, 1839
SacI GAGCTC 1 cut(s) 1146
SalI GTCGAC 1 cut(s) 730
SaqAI TTAA 5 cut(s) 483, 1077, 1539, 1682, 1853
Sau96I GGNCC 4 cut(s) 406, 499, 574, 977
ScaI AGTACT 5 cut(s) 42, 919, 925, 1270, 1676
SchI GAGTC 3 cut(s) 619, 1097, 1917
ScrFI CCNGG 9 cut(s) 208, 410, 411, 424, 503, 727, 737, 981, 1628
SduI GDGCHC 2 cut(s) 393, 1146
SfaNI GCATC 4 cut(s) 1333, 1383, 1510, 1621
SfcI CTRYAG 1 cut(s) 36
SfoI GGCGCC 2 cut(s) 205, 1668
SfuI TTCGAA 1 cut(s) 641
SgrAI CRCCGGYG 1 cut(s) 374
SinI GGWCC 3 cut(s) 406, 574, 977
SmaI CCCGGG 1 cut(s) 411
SmiMI CAYNNNNRTG 5 cut(s) 458, 464, 1444, 1533, 1839
SmlI CTYRAG 4 cut(s) 128, 563, 1145, 1736
SmoI CTYRAG 4 cut(s) 128, 563, 1145, 1736
SpeI ACTAGT 2 cut(s) 920, 1915
Sse9I AATT 8 cut(s) 95, 301, 583, 596, 1608, 1761, 1883, 2007
SspDI GGCGCC 2 cut(s) 203, 1666
SspI AATATT 1 cut(s) 1221
SspMI CTAG 9 cut(s) 267, 915, 921, 1092, 1431, 1602, 1746, 1916, 1985
SstI GAGCTC 1 cut(s) 1146
StyD4I CCNGG 9 cut(s) 206, 408, 409, 422, 501, 725, 735, 979, 1626
StyI CCWWGG 2 cut(s) 516, 1868
TaaI ACNGT 4 cut(s) 37, 941, 2075, 2138
TaiI ACGT 6 cut(s) 83, 290, 468, 498, 699, 717
TasI AATT 8 cut(s) 95, 301, 583, 596, 1608, 1761, 1883, 2007
TatI WGTACW 6 cut(s) 40, 219, 917, 923, 1268, 1674
TauI GCSGC 3 cut(s) 327, 826, 1646
TfiI GAWTC 6 cut(s) 13, 643, 791, 1174, 1465, 1516
Tru1I TTAA 5 cut(s) 483, 1077, 1539, 1682, 1853
Tru9I TTAA 5 cut(s) 483, 1077, 1539, 1682, 1853
TscAI CASTG 4 cut(s) 1254, 1734, 1783, 2080
TseFI GTSAC 2 cut(s) 1000, 1235
TseI GCWGC 8 cut(s) 123, 162, 548, 815, 1151, 1640, 1796, 1859
Tsp45I GTSAC 2 cut(s) 1000, 1235
TspDTI ATGAA 4 cut(s) 697, 860, 1578, 1857
TspGWI ACGGA 5 cut(s) 25, 246, 283, 619, 1721
TspMI CCCGGG 1 cut(s) 409
TspRI CASTG 4 cut(s) 1254, 1734, 1783, 2080
Van91I CCANNNNNTGG 1 cut(s) 400
VpaK11BI GGWCC 3 cut(s) 406, 574, 977
VspI ATTAAT 1 cut(s) 483
XapI RAATTY 2 cut(s) 95, 596
XceI RCATGY 1 cut(s) 1076
XcmI CCANNNNNNNNNTGG 1 cut(s) 155
XmaI CCCGGG 1 cut(s) 409
XmiI GTMKAC 1 cut(s) 731
XmnI GAANNNNTTC 1 cut(s) 1163
XspI CTAG 9 cut(s) 267, 915, 921, 1092, 1431, 1602, 1746, 1916, 1985
ZrmI AGTACT 5 cut(s) 42, 919, 925, 1270, 1676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.