MD04G1163000.v1.1

Protein of unknown function (DUF688)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0015801
Physical Location & Seq
Forward (+)
25451744 .. 25452520
777 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1163000.v1.1.491

Sequence Viewer

Length: 777 bp
ATGCTGAACTTCAATGCACCACTCCTCTCAACCCGCCGCCTTGGAAACTCCGGCGGTGAAGATTTACAAGGTGCATCGCTGCCAGCGAGCGAGCGGATTCCGTTTTCCTGGGAACGAGTCCCTGGCAAGCCCAAGGACTCGAATGCGGGTGACGATATCCAGCCTGGGGACACTCCTCGCCCAAAGCTTCCCCCAGGCTGGTGGCACCGGCCTCTTGACAAGGACAACGAGTGTGATCATGAACTCGATGATGGATGTGAGGGCGACATTGATGACGATGATGATCTACGTGACCTTGAGGATGATATCTACGATGTTGATTACAGCAACGAGGCCTTCTCGGATGCCATAGATGTGTTCTCACTCTCAGAGGCAATAGACATTGTGGAAAAGAAAGCACAGAGAGAACAAGAAACAGATCACAGCTTGAAGTTGAAGCTTGCAGAAGAATCAAGGCACGAGATGAATCAAAACTGTCCGAATTTCATAATCGAGCGGTTTCTTCCGGACGCAACTGCATTGGCTGCACCTTCTGCTCTGAAGCTAAACGTGTCACAGATGAGTTCAAGCAAGAGACTCTGCTACCCGTGGAATTATCCGGAGCCGGCGGAGGCCTGTGTTTCGAGAACGGTGTGCAGGCAGTCGTACTCGTCGCCCAAGGGTTGTGGCTTGCAAGGTTTCTTCCCTTGGCGGATGAAGCATAAGCTCTGTCGAGCGAAAAGCCCTGTCGATGCACAAGCTCAATGCAGTGTAAAACACAAGAAAAATAGATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF688

Protein Analysis

259

Amino Acids

29.01

Weight (kDa)

5.01

Isoelectric Point (pI)

54.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF688 PF05097 33 - 178 5.6e-09 Protein of unknown function (DUF688)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015801)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51760 AT3G51760 AT3G51760 AT3G51760
malus_domestica MD04G1163000.v1.1
prunus_persica Prupe.6G282500_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0462281
rosa_multiflora Rmu_co8387589.1_g000001 Rmu_sc0001553.1_g000001
rosa_roxburghii Rroxscaffold_6G00417620
rosa_samantha Rh3AG114200 Rh3BG117300 Rh3CG120100 Rh3DG119100
rosa_wichuraiana Rw3G009640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 204
AccBSI CCGCTC 2 cut(s) 94, 496
AccIII TCCGGA 2 cut(s) 505, 598
AciI CCGC 8 cut(s) 34, 37, 54, 94, 146, 496, 608, 691
AcsI RAATTY 1 cut(s) 481
AcuI CTGAAG 1 cut(s) 560
AfaI GTAC 1 cut(s) 647
AfiI CCNNNNNNNGG 2 cut(s) 166, 198
AflIII ACRYGT 1 cut(s) 549
AgsI TTSAA 4 cut(s) 13, 430, 436, 567
AjnI CCWGG 4 cut(s) 107, 121, 163, 193
AluBI AGCT 6 cut(s) 187, 426, 439, 544, 706, 740
AluI AGCT 6 cut(s) 187, 426, 439, 544, 706, 740
Alw26I GTCTC 1 cut(s) 568
Aor13HI TCCGGA 2 cut(s) 505, 598
AoxI GGCC 3 cut(s) 209, 333, 612
ApeKI GCWGC 2 cut(s) 79, 524
ApoI RAATTY 1 cut(s) 481
AsuHPI GGTGA 2 cut(s) 68, 161
BanI GGYRCC 1 cut(s) 204
BauI CACGAG 1 cut(s) 458
BbvI GCAGC 2 cut(s) 66, 511
BccI CCATC 1 cut(s) 245
BciT130I CCWGG 4 cut(s) 109, 123, 165, 195
BclI TGATCA 1 cut(s) 235
BcoDI GTCTC 1 cut(s) 568
BisI GCNGC 3 cut(s) 37, 80, 525
BlsI GCNGC 3 cut(s) 38, 81, 526
Bme1390I CCNGG 4 cut(s) 109, 123, 165, 195
BmiI GGNNCC 2 cut(s) 206, 603
BmrFI CCNGG 4 cut(s) 109, 123, 165, 195
BmsI GCATC 3 cut(s) 83, 334, 721
BplI GAGNNNNNCTC 2 cut(s) 323, 355
BpuEI CTTGAG 1 cut(s) 317
BsaAI YACGTR 1 cut(s) 290
BsaBI GATNNNNATC 1 cut(s) 282
BsaJI CCNNGG 9 cut(s) 40, 108, 121, 132, 164, 193, 587, 657, 686
BsaWI WCCGGW 2 cut(s) 505, 598
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 198
Bse118I RCCGGY 2 cut(s) 207, 604
Bse8I GATNNNNATC 1 cut(s) 282
BseAI TCCGGA 2 cut(s) 505, 598
BseBI CCWGG 4 cut(s) 109, 123, 165, 195
BseDI CCNNGG 9 cut(s) 40, 108, 121, 132, 164, 193, 587, 657, 686
BseGI GGATG 4 cut(s) 260, 307, 349, 699
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 2 cut(s) 166, 198
BseMII CTCAG 1 cut(s) 381
BseRI GAGGAG 2 cut(s) 14, 165
BseXI GCAGC 2 cut(s) 66, 511
BsgI GTGCAG 2 cut(s) 510, 655
BshFI GGCC 3 cut(s) 211, 335, 614
BshNI GGYRCC 1 cut(s) 204
BsiSI CCGG 5 cut(s) 51, 208, 506, 599, 605
BslFI GGGAC 2 cut(s) 104, 182
BslI CCNNNNNNNGG 2 cut(s) 166, 198
BsmAI GTCTC 1 cut(s) 568
BsmFI GGGAC 2 cut(s) 104, 182
BsmI GAATGC 1 cut(s) 148
BsnI GGCC 3 cut(s) 211, 335, 614
Bsp13I TCCGGA 2 cut(s) 505, 598
Bsp143I GATC 4 cut(s) 235, 283, 418, 770
BspACI CCGC 8 cut(s) 34, 37, 54, 94, 146, 496, 608, 691
BspANI GGCC 3 cut(s) 211, 335, 614
BspCNI CTCAG 1 cut(s) 380
BspEI TCCGGA 2 cut(s) 505, 598
BspHI TCATGA 2 cut(s) 238, 773
BspLI GGNNCC 2 cut(s) 206, 603
BspT107I GGYRCC 1 cut(s) 204
BsrBI CCGCTC 2 cut(s) 94, 496
BsrFI RCCGGY 2 cut(s) 207, 604
BssAI RCCGGY 2 cut(s) 207, 604
BssECI CCNNGG 9 cut(s) 40, 108, 121, 132, 164, 193, 587, 657, 686
BssMI GATC 4 cut(s) 235, 283, 418, 770
BssSI CACGAG 1 cut(s) 458
BssT1I CCWWGG 4 cut(s) 40, 132, 657, 686
Bst2BI CACGAG 1 cut(s) 458
Bst2UI CCWGG 4 cut(s) 109, 123, 165, 195
Bst4CI ACNGT 2 cut(s) 476, 631
BstAPI GCANNNNNTGC 2 cut(s) 524, 533
BstBAI YACGTR 1 cut(s) 290
BstC8I GCNNGC 8 cut(s) 84, 88, 92, 128, 441, 606, 638, 671
BstDEI CTNAG 1 cut(s) 367
BstDSI CCRYGG 1 cut(s) 587
BstF5I GGATG 4 cut(s) 260, 307, 349, 699
BstKTI GATC 4 cut(s) 238, 286, 421, 773
BstMAI GTCTC 1 cut(s) 568
BstMBI GATC 4 cut(s) 235, 283, 418, 770
BstMWI GCNNNNNNNGC 3 cut(s) 524, 533, 697
BstNI CCWGG 4 cut(s) 109, 123, 165, 195
BstSCI CCNGG 4 cut(s) 107, 121, 163, 193
BstV1I GCAGC 2 cut(s) 66, 511
BstXI CCANNNNNNTGG 1 cut(s) 201
BsuRI GGCC 3 cut(s) 211, 335, 614
BtgI CCRYGG 1 cut(s) 587
BtgZI GCGATG 1 cut(s) 60
BtsCI GGATG 4 cut(s) 260, 307, 349, 699
BtsI GCAGTG 1 cut(s) 754
BtsIMutI CAGTG 1 cut(s) 754
Cac8I GCNNGC 8 cut(s) 84, 88, 92, 128, 441, 606, 638, 671
CciI TCATGA 2 cut(s) 238, 773
Cfr10I RCCGGY 2 cut(s) 207, 604
CseI GACGC 1 cut(s) 518
Csp6I GTAC 1 cut(s) 646
CspCI CAANNNNNGTGG 2 cut(s) 646, 681
CviAII CATG 2 cut(s) 239, 774
CviQI GTAC 1 cut(s) 646
DdeI CTNAG 1 cut(s) 367
DpnI GATC 4 cut(s) 237, 285, 420, 772
DpnII GATC 4 cut(s) 235, 283, 418, 770
EciI GGCGGA 2 cut(s) 623, 706
Eco130I CCWWGG 4 cut(s) 40, 132, 657, 686
Eco147I AGGCCT 2 cut(s) 335, 614
Eco32I GATATC 2 cut(s) 157, 307
Eco57I CTGAAG 1 cut(s) 560
EcoRII CCWGG 4 cut(s) 107, 121, 163, 193
EcoRV GATATC 2 cut(s) 157, 307
EcoT14I CCWWGG 4 cut(s) 40, 132, 657, 686
ErhI CCWWGG 4 cut(s) 40, 132, 657, 686
FaeI CATG 2 cut(s) 242, 777
FaiI YATR 5 cut(s) 240, 350, 488, 702, 775
FaqI GGGAC 2 cut(s) 104, 182
FatI CATG 2 cut(s) 238, 773
FauI CCCGC 2 cut(s) 41, 139
FbaI TGATCA 1 cut(s) 235
Fnu4HI GCNGC 3 cut(s) 37, 80, 525
FokI GGATG 4 cut(s) 267, 314, 356, 706
Fsp4HI GCNGC 3 cut(s) 37, 80, 525
GluI GCNGC 3 cut(s) 37, 80, 525
HaeIII GGCC 3 cut(s) 211, 335, 614
HapII CCGG 5 cut(s) 51, 208, 506, 599, 605
HgaI GACGC 1 cut(s) 518
Hin1II CATG 2 cut(s) 242, 777
HindIII AAGCTT 2 cut(s) 185, 437
HinfI GANTC 6 cut(s) 97, 117, 137, 449, 466, 576
HpaII CCGG 5 cut(s) 51, 208, 506, 599, 605
HphI GGTGA 2 cut(s) 68, 161
Hpy188I TCNGA 4 cut(s) 343, 370, 480, 540
Hpy188III TCNNGA 6 cut(s) 215, 239, 506, 599, 624, 774
Hpy99I CGWCG 1 cut(s) 655
HpyAV CCTTC 2 cut(s) 346, 540
HpyCH4III ACNGT 2 cut(s) 476, 631
HpyCH4IV ACGT 2 cut(s) 289, 549
HpyCH4V TGCA 9 cut(s) 17, 74, 443, 518, 527, 636, 673, 734, 747
HpyF10VI GCNNNNNNNGC 3 cut(s) 524, 533, 697
HpyF3I CTNAG 1 cut(s) 367
HpySE526I ACGT 2 cut(s) 289, 549
Hsp92II CATG 2 cut(s) 242, 777
Kpn2I TCCGGA 2 cut(s) 505, 598
KroI GCCGGC 1 cut(s) 604
KroNI GCCGGC 1 cut(s) 606
Ksp22I TGATCA 1 cut(s) 235
Kzo9I GATC 4 cut(s) 235, 283, 418, 770
LmnI GCTCC 1 cut(s) 601
Lsp1109I GCAGC 2 cut(s) 66, 511
LweI GCATC 3 cut(s) 83, 334, 721
MaeII ACGT 2 cut(s) 289, 549
MaeIII GTNAC 3 cut(s) 149, 290, 552
MalI GATC 4 cut(s) 237, 285, 420, 772
MbiI CCGCTC 2 cut(s) 94, 496
MboI GATC 4 cut(s) 235, 283, 418, 770
MboII GAAGA 4 cut(s) 71, 458, 494, 673
MluCI AATT 2 cut(s) 481, 592
MlyI GAGTC 3 cut(s) 126, 131, 570
MnlI CCTC 8 cut(s) 35, 186, 222, 253, 292, 325, 364, 604
MroI TCCGGA 2 cut(s) 505, 598
MroNI GCCGGC 1 cut(s) 604
MslI CAYNNNNRTG 1 cut(s) 353
MspI CCGG 5 cut(s) 51, 208, 506, 599, 605
MspR9I CCNGG 4 cut(s) 109, 123, 165, 195
Mva1269I GAATGC 1 cut(s) 148
MvaI CCWGG 4 cut(s) 109, 123, 165, 195
MwoI GCNNNNNNNGC 3 cut(s) 524, 533, 697
NaeI GCCGGC 1 cut(s) 606
NdeII GATC 4 cut(s) 235, 283, 418, 770
NgoMIV GCCGGC 1 cut(s) 604
NlaIII CATG 2 cut(s) 242, 777
NlaIV GGNNCC 2 cut(s) 206, 603
NmuCI GTSAC 3 cut(s) 149, 290, 552
PagI TCATGA 2 cut(s) 238, 773
PceI AGGCCT 2 cut(s) 335, 614
PcsI WCGNNNNNNNCGW 1 cut(s) 83
PctI GAATGC 1 cut(s) 148
PdiI GCCGGC 1 cut(s) 606
PfeI GAWTC 3 cut(s) 97, 449, 466
PkrI GCNGC 3 cut(s) 38, 81, 526
PleI GAGTC 3 cut(s) 125, 131, 570
PpsI GAGTC 3 cut(s) 125, 131, 570
Ppu21I YACGTR 1 cut(s) 290
Psp6I CCWGG 4 cut(s) 107, 121, 163, 193
PspGI CCWGG 4 cut(s) 107, 121, 163, 193
PspN4I GGNNCC 2 cut(s) 206, 603
RsaI GTAC 1 cut(s) 647
RsaNI GTAC 1 cut(s) 646
RseI CAYNNNNRTG 1 cut(s) 353
SatI GCNGC 3 cut(s) 37, 80, 525
Sau3AI GATC 4 cut(s) 235, 283, 418, 770
SchI GAGTC 3 cut(s) 126, 131, 570
ScrFI CCNGG 4 cut(s) 109, 123, 165, 195
SfaNI GCATC 3 cut(s) 83, 334, 721
SmiMI CAYNNNNRTG 1 cut(s) 353
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 2 cut(s) 481, 592
SseBI AGGCCT 2 cut(s) 335, 614
SsiI CCGC 8 cut(s) 34, 37, 54, 94, 146, 496, 608, 691
StuI AGGCCT 2 cut(s) 335, 614
StyD4I CCNGG 4 cut(s) 107, 121, 163, 193
StyI CCWWGG 4 cut(s) 40, 132, 657, 686
TaaI ACNGT 2 cut(s) 476, 631
TaiI ACGT 2 cut(s) 292, 552
TaqI TCGA 6 cut(s) 140, 246, 492, 623, 712, 729
TasI AATT 2 cut(s) 481, 592
TauI GCSGC 1 cut(s) 39
TfiI GAWTC 3 cut(s) 97, 449, 466
TscAI CASTG 1 cut(s) 754
TseFI GTSAC 3 cut(s) 149, 290, 552
TseI GCWGC 2 cut(s) 79, 524
Tsp45I GTSAC 3 cut(s) 149, 290, 552
TspDTI ATGAA 4 cut(s) 255, 475, 479, 710
TspGWI ACGGA 1 cut(s) 90
TspRI CASTG 1 cut(s) 754
XapI RAATTY 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.