MD04G1174900.v1.1
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009915
Physical Location & Seq
Reverse (-)
26617142 .. 26621239
4098 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1174900.v1.1.491

Sequence Viewer

Length: 2898 bp
ATGGCGTTTGAGCTCTGTTTTCTGCTTCTTCTTGCTCCTCTTCTGGTACTCGCCCTGGACCCCGTTTTCAACGACGACGTTCTGGGCCTGATCGTCTTCAAGGCGGGACTTTCCGACCCGGAAGCCAAGCTCGCCTCTTGGAACGAGGACGACGACAGTCCCTGCAGCTGGGTCGGAGTCAAATGCGACCCGACAACCGGCAGAGTCGCCGAGGTTGTTCTCGACGGATTTTCGCTATCCGGGCACATCGGCCGCGGCCTTTTGCGGCTGCAGAATCTCCAAAGACTCTCGCTTTCCGACAACAACTTCACTGGGTTCATAAACCCGGATCTCCCACACCTCGGGAGCTTGCAGGTTATCGATTTGAGCCGGAATAGCCTATCTGGGTCGGTCCCGGAGGAGTTTTTCATGCAATGTGGGTCTCTCAGGGTGGTTTCTTTCGCCAGAAACAACCTCACTGGGCGGATTCCAGAGTCCCTGAGCTTGTGCCAGACGTTGGTGGATGTGAACTTTTCGTCGAATCGGATGTCCGGGAAGTTGCCGTCTGGGATTTGGTACTTGAGGATGCTTCAGTCGCTAGACTTGGCGGATAACTTGGTGGAGGGTGAAGTTCCTGAAGGTATTGAGAATTTGTATGATTTGAGAGTGATTAACTTGGCAAAAAACCGATTTTCAGGACAGCTTCCTGGGGACTTTGGAAGCTGTTTGCAGTTGAAGTTGCTTGATTTTAGTGAGAATTTGTTTTCTGGAAGAATACCGGATTCGATTCAGAGGCTCAATTCATGCACTTCTCTGAGCTTACAGGGGAATTTGCTTGCGGGGCAAGTTCCGAATTGGCTTGGCGATTTGAAGAACCTTGAGGTGTTGGATGTGTCTGGTAATAATTTCTCTGGTGAAATTCCAAGTTCATTAGGAAATCTCGAGCTACTCGAAAAGTTGAACTTGTCTAGGAATGGATTTACTGGAAGCTTGCCAGATACGTTGGCAAATTGCAACAATCTGCTAGCCATAGATGTTAGCCATAATCTGTTAGCAGGTAAGCTTCCTTCGTGGATTTTTAAGCTCGGTCTGCGCAGTGTTTCGCTTTATGGCAACCGATTAGGTGGAAGTGAGGAGTACAGTTCATTAGCGTCAATGGCAGCTTCTAATGGAGGCCTGCAGGTCTTGGATTTGTCATCGAACGCGTTTTCTGATGTGCTTCCATCCGACATTGGTGTTCTTAGTAGCTTGCAGTTTTTGAATATGTCAGGGAACCATCTGTTGGGTTGGATTCCAGCAAGTATAGGTGAATTAAAGGCGGCATATGTTCTCGACTTGAGTGACAATTGGCTCAATGGAAGCATTCCTGATGAAATCGGAGGGGCAGTCTCGCTCAAGGAAATAAGACTGCAGAAGAACTTTCTAAGCGGGAAAGTTCCAGCCGAAATTGCAAAGTGCTCATCTCTGACAAATCTGATTCTATCGCAGAACAATCTGACTGGCCCTATTCCTACAGCCCTTGCTAACCTTACCAATCTTCAATATGTCGACTTGTCTTTGAACAAATTCTCAGGAGGCATACCGAAAGAGCTGACAAATCTTTCCCATCTCCTCTACTTCAATGTCTCCCACAACCACCTTGAGGGTGAACTGCCATTAGGGGGATTCTTCAACACTATCCCGCCGTCATCTGTCTCAGACAACCCATCCCTATGTGGATCTGCTGTTACCCGTGCCTGCCCTTCCGTTCACCCCAAACCCATTGTCCTCAACCCTAATTCATCCAACCCCGTCCATGGTTCTTCCTCTCCAACTCATGGTCACAAGATCGTATTCAGCATATCAGCTCTCATAGCTATTGGTGCAGCTGTATTCATTGCCATTGGTGTCATAGCTATAACGGTCCTCAATATGCACGTGCGGTCTTCATTGTCACGTTCTGCTGCTCCTCTTGAGTTATCAGGCGGGGAAGATTATAGTTGTTCCCCTGCTAATGATCCAAACTACGGCAAGCTTGTCATGTTTTCTGGTGATGCCGACTTTGGTGCTGGGGCACAAGCCTTGCTCAACAAGGACTGCGAACTTGGTCGTGGTGGGTTTGGAGTAGTTTACAAAACAGCCCTTCGTGATGGGCGTTCAGTTGCGATAAAAAAGCTAACAGTCTCAAGTTTGATCAAGTCCCAAGAAGATTTCGAGAGGGAAGTTAAAGGACTTGGAAAGATCAGGCACCGTAATCTTGTGGCCCTAGAAGGGTATTACTGGACTCCGTCCTTGCAGCTCATCATTTATGAATACATACCATGCGGGAGTCTGTTTAAGAATCTGCATGACGGACCTGGCAAAACCTGCCTCACCTGGCGGCAGAGGTTCCACATAATTCTCGGGATGGCAAAGGGTTTGGCTCATTTGCACCAAATGAAGATAATCCACTACAATTTGAAATCAACTAATGTTTTGATAGACAGCAATGGCGAGCCTAAGGTGGGAGACTTTGGCTTGGCAAGGCTGTTGCCAACACTAGACCGGTGCATCTTAAGCAGCAAAATCCAAAGCGCGCTTGGCTACATGGCGCCTGAGTTTGCATGTCAAACGGTTAAGATTACTGAGAAATGTGATGTGTATGGATTTGGAATCTTGGTTTTGGAGGTGGTGACTGGAAAGAGGCCTGTCGAATATTTGGAGGATGACGTGATAGTACTCTGTGACATGGTGAGGGGAGCGCTCGAGGAAGGCCGGGTGGAGGAATGCCTTGACAAAAATCTCCTTGGCAATTTTCCAGCAGAGGAGGCAATTCCGGTGGTAAAGCTGGGTTTAATATGCGCGTCTCAAGTGCCATCAAATCGCCCGGACATGAGTGAAGTGATCAACATTTTAGAACTCATCCAATGCCCTTCAGAAGGCCATGAAGAATTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

966

Amino Acids

104.22

Weight (kDa)

5.29

Isoelectric Point (pI)

38.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 63 2.8e-13 Leucine rich repeat N-terminal domain
LRR_14 PF23598 184 - 352 3.8e-10 Leucine-rich repeat region
LRR_14 PF23598 386 - 518 9e-08 Leucine-rich repeat region
LRR_8 PF13855 481 - 540 5.6e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 684 - 950 1.9e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 685 - 889 8.5e-41 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1073
Acc36I ACCTGC 4 cut(s) 343, 1025, 1150, 2333
AccB1I GGYRCC 2 cut(s) 2205, 2548
AccB7I CCANNNNNTGG 3 cut(s) 496, 1261, 1796
AccI GTMKAC 1 cut(s) 1527
AccII CGCG 4 cut(s) 255, 1184, 2534, 2801
AclWI GGATC 3 cut(s) 336, 1705, 1970
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 5 cut(s) 628, 736, 808, 897, 1544
AcuI CTGAAG 3 cut(s) 554, 636, 2856
AcvI CACGTG 1 cut(s) 1897
AcyI GRCGYC 1 cut(s) 2549
AfaI GTAC 4 cut(s) 48, 557, 1118, 2676
AfeI AGCGCT 1 cut(s) 2700
AflII CTTAAG 1 cut(s) 2512
AflIII ACRYGT 1 cut(s) 1182
AgeI ACCGGT 1 cut(s) 2502
AjiI CACGTC 1 cut(s) 2668
AjnI CCWGG 4 cut(s) 54, 685, 2314, 2333
AjuI GAANNNNNNNTTGG 2 cut(s) 1244, 1276
Alw21I GWGCWC 2 cut(s) 15, 1439
Alw26I GTCTC 7 cut(s) 426, 1372, 1609, 1678, 2146, 2460, 2808
AlwI GGATC 3 cut(s) 336, 1705, 1970
AlwNI CAGNNNCTG 1 cut(s) 162
Ama87I CYCGRG 4 cut(s) 341, 920, 2360, 2702
Aor51HI AGCGCT 1 cut(s) 2700
AoxI GGCC 9 cut(s) 85, 250, 256, 1153, 1480, 2220, 2642, 2710, 2878
ApeKI GCWGC 7 cut(s) 165, 268, 1139, 1844, 1922, 2254, 2517
ApoI RAATTY 5 cut(s) 628, 736, 808, 897, 1544
ArsI GACNNNNNNTTYG 4 cut(s) 512, 544, 1728, 1760
AsiGI ACCGGT 1 cut(s) 2502
Asp700I GAANNNNTTC 1 cut(s) 1544
AspLEI GCGC 6 cut(s) 1074, 2534, 2536, 2551, 2701, 2801
AspS9I GGNCC 7 cut(s) 58, 85, 391, 1481, 1882, 2221, 2312
AsuC2I CCSGG 7 cut(s) 119, 241, 326, 395, 532, 2714, 2825
AsuHPI GGTGA 9 cut(s) 617, 905, 1298, 1637, 1721, 2021, 2323, 2641, 2701
AsuNHI GCTAGC 1 cut(s) 1003
AvaI CYCGRG 4 cut(s) 341, 920, 2360, 2702
AvaII GGWCC 4 cut(s) 58, 391, 1882, 2312
AxyI CCTNAGG 1 cut(s) 2457
BaeGI GKGCMC 2 cut(s) 246, 2035
BanI GGYRCC 2 cut(s) 2205, 2548
BanII GRGCYC 1 cut(s) 15
BarI GAAGNNNNNNTAC 2 cut(s) 1030, 1062
BbrPI CACGTG 1 cut(s) 1897
BbsI GAAGAC 2 cut(s) 88, 1896
Bbv12I GWGCWC 2 cut(s) 15, 1439
BbvI GCAGC 7 cut(s) 177, 255, 1151, 1856, 1909, 2266, 2529
BccI CCATC 7 cut(s) 1210, 1263, 1593, 1693, 2102, 2359, 2821
BceAI ACGGC 3 cut(s) 526, 1648, 2002
BcgI CGANNNNNNTGC 4 cut(s) 154, 188, 2006, 2040
BciT130I CCWGG 4 cut(s) 56, 687, 2316, 2335
BclI TGATCA 2 cut(s) 2151, 2841
BcnI CCSGG 7 cut(s) 119, 241, 326, 395, 532, 2714, 2825
BcoDI GTCTC 7 cut(s) 426, 1372, 1609, 1678, 2146, 2460, 2808
BfaI CTAG 5 cut(s) 578, 948, 1004, 2225, 2498
BfmI CTRYAG 5 cut(s) 163, 269, 1157, 1388, 1491
BfoI RGCGCY 2 cut(s) 2552, 2702
BfrI CTTAAG 1 cut(s) 2512
BfuAI ACCTGC 4 cut(s) 343, 1025, 1150, 2333
BmcAI AGTACT 1 cut(s) 2676
Bme18I GGWCC 4 cut(s) 58, 391, 1882, 2312
BmeT110I CYCGRG 4 cut(s) 341, 920, 2360, 2702
BmgBI CACGTC 1 cut(s) 2668
BmgT120I GGNCC 7 cut(s) 58, 85, 391, 1481, 1882, 2221, 2312
BmiI GGNNCC 6 cut(s) 60, 393, 1253, 2207, 2348, 2550
BmrI ACTGGG 2 cut(s) 321, 468
BmsI GCATC 3 cut(s) 555, 2002, 2517
BmtI GCTAGC 1 cut(s) 1007
BmuI ACTGGG 2 cut(s) 321, 468
BoxI GACNNNNGTC 1 cut(s) 156
BpiI GAAGAC 2 cut(s) 88, 1896
Bpu10I CCTNAGC 1 cut(s) 479
BpuEI CTTGAG 8 cut(s) 580, 878, 1336, 1358, 1640, 1952, 2128, 2790
BpuMI CCSGG 7 cut(s) 119, 241, 326, 395, 532, 2714, 2825
Bsa29I ATCGAT 1 cut(s) 360
BsaAI YACGTR 1 cut(s) 1897
BsaHI GRCGYC 1 cut(s) 2549
BsaI GGTCTC 1 cut(s) 426
BsaJI CCNNGG 7 cut(s) 54, 210, 253, 340, 686, 1774, 2743
BsaWI WCCGGW 3 cut(s) 757, 2502, 2773
BsaXI ACNNNNNCTCC 2 cut(s) 1350, 1380
Bse118I RCCGGY 2 cut(s) 197, 2502
Bse1I ACTGG 6 cut(s) 316, 463, 967, 1483, 2243, 2638
Bse21I CCTNAGG 1 cut(s) 2457
Bse3DI GCAATG 3 cut(s) 419, 1854, 2452
BseBI CCWGG 4 cut(s) 56, 687, 2316, 2335
BseCI ATCGAT 1 cut(s) 360
BseDI CCNNGG 7 cut(s) 54, 210, 253, 340, 686, 1774, 2743
BseMI GCAATG 3 cut(s) 419, 1854, 2452
BseMII CTCAG 7 cut(s) 439, 470, 785, 1563, 1689, 2544, 2574
BseNI ACTGG 6 cut(s) 316, 463, 967, 1483, 2243, 2638
BsePI GCGCGC 1 cut(s) 2532
BseRI GAGGAG 6 cut(s) 27, 413, 1127, 1580, 1917, 2777
BseSI GKGCMC 2 cut(s) 246, 2035
BseX3I CGGCCG 1 cut(s) 250
BseXI GCAGC 7 cut(s) 177, 255, 1151, 1856, 1909, 2266, 2529
BseYI CCCAGC 3 cut(s) 168, 2027, 2785
BsgI GTGCAG 1 cut(s) 1863
Bsh1236I CGCG 4 cut(s) 255, 1184, 2534, 2801
Bsh1285I CGRYCG 1 cut(s) 253
BshFI GGCC 9 cut(s) 87, 252, 258, 1155, 1482, 2222, 2644, 2712, 2880
BshNI GGYRCC 2 cut(s) 2205, 2548
BshTI ACCGGT 1 cut(s) 2502
BshVI ATCGAT 1 cut(s) 360
BsiEI CGRYCG 1 cut(s) 253
BsiHKAI GWGCWC 2 cut(s) 15, 1439
BsiHKCI CYCGRG 4 cut(s) 341, 920, 2360, 2702
BslFI GGGAC 6 cut(s) 120, 144, 377, 460, 704, 2143
BsmAI GTCTC 7 cut(s) 426, 1372, 1609, 1678, 2146, 2460, 2808
BsmBI CGTCTC 1 cut(s) 2808
BsmFI GGGAC 6 cut(s) 120, 144, 377, 460, 704, 2143
BsmI GAATGC 2 cut(s) 1341, 2729
BsnI GGCC 9 cut(s) 87, 252, 258, 1155, 1482, 2222, 2644, 2712, 2880
Bso31I GGTCTC 1 cut(s) 426
BsoBI CYCGRG 4 cut(s) 341, 920, 2360, 2702
Bsp1286I GDGCHC 4 cut(s) 15, 246, 1439, 2035
Bsp143I GATC 8 cut(s) 90, 328, 1697, 1806, 1975, 2151, 2199, 2841
Bsp19I CCATGG 1 cut(s) 1774
BspANI GGCC 9 cut(s) 87, 252, 258, 1155, 1482, 2222, 2644, 2712, 2880
BspCNI CTCAG 7 cut(s) 438, 471, 786, 1562, 1688, 2545, 2575
BspDI ATCGAT 1 cut(s) 360
BspFNI CGCG 4 cut(s) 255, 1184, 2534, 2801
BspLI GGNNCC 6 cut(s) 60, 393, 1253, 2207, 2348, 2550
BspMAI CTGCAG 4 cut(s) 167, 273, 1161, 1392
BspMI ACCTGC 4 cut(s) 343, 1025, 1150, 2333
BspOI GCTAGC 1 cut(s) 1007
BspPI GGATC 3 cut(s) 336, 1705, 1970
BspT107I GGYRCC 2 cut(s) 2205, 2548
BspTI CTTAAG 1 cut(s) 2512
BspTNI GGTCTC 1 cut(s) 426
BsrDI GCAATG 3 cut(s) 419, 1854, 2452
BsrFI RCCGGY 2 cut(s) 197, 2502
BsrI ACTGG 6 cut(s) 316, 463, 967, 1483, 2243, 2638
BssAI RCCGGY 2 cut(s) 197, 2502
BssECI CCNNGG 7 cut(s) 54, 210, 253, 340, 686, 1774, 2743
BssHII GCGCGC 1 cut(s) 2532
BssMI GATC 8 cut(s) 90, 328, 1697, 1806, 1975, 2151, 2199, 2841
BssNI GRCGYC 1 cut(s) 2549
BssT1I CCWWGG 2 cut(s) 1774, 2743
Bst2UI CCWGG 4 cut(s) 56, 687, 2316, 2335
Bst4CI ACNGT 6 cut(s) 158, 1121, 1882, 2140, 2210, 2572
Bst6I CTCTTC 1 cut(s) 45
BstACI GRCGYC 1 cut(s) 2549
BstAFI CTTAAG 1 cut(s) 2512
BstAPI GCANNNNNTGC 1 cut(s) 2325
BstBAI YACGTR 1 cut(s) 1897
BstDSI CCRYGG 2 cut(s) 253, 1774
BstENI CCTNNNNNAGG 1 cut(s) 2874
BstFNI CGCG 4 cut(s) 255, 1184, 2534, 2801
BstH2I RGCGCY 2 cut(s) 2552, 2702
BstHHI GCGC 6 cut(s) 1074, 2534, 2536, 2551, 2701, 2801
BstKTI GATC 8 cut(s) 93, 331, 1700, 1809, 1978, 2154, 2202, 2844
BstMAI GTCTC 7 cut(s) 426, 1372, 1609, 1678, 2146, 2460, 2808
BstMBI GATC 8 cut(s) 90, 328, 1697, 1806, 1975, 2151, 2199, 2841
BstMCI CGRYCG 1 cut(s) 253
BstNI CCWGG 4 cut(s) 56, 687, 2316, 2335
BstNSI RCATGY 1 cut(s) 2565
BstPAI GACNNNNGTC 1 cut(s) 156
BstSFI CTRYAG 5 cut(s) 163, 269, 1157, 1388, 1491
BstSLI GKGCMC 2 cut(s) 246, 2035
BstUI CGCG 4 cut(s) 255, 1184, 2534, 2801
BstV1I GCAGC 7 cut(s) 177, 255, 1151, 1856, 1909, 2266, 2529
BstV2I GAAGAC 2 cut(s) 88, 1896
BstX2I RGATCY 2 cut(s) 328, 1697
BstYI RGATCY 2 cut(s) 328, 1697
BstZI CGGCCG 1 cut(s) 250
Bsu15I ATCGAT 1 cut(s) 360
Bsu36I CCTNAGG 1 cut(s) 2457
BsuRI GGCC 9 cut(s) 87, 252, 258, 1155, 1482, 2222, 2644, 2712, 2880
BsuTUI ATCGAT 1 cut(s) 360
BtgI CCRYGG 2 cut(s) 253, 1774
BtrI CACGTC 1 cut(s) 2668
BtsI GCAGTG 1 cut(s) 1081
BtsIMutI CAGTG 3 cut(s) 309, 456, 1081
BveI ACCTGC 4 cut(s) 343, 1025, 1150, 2333
CaiI CAGNNNCTG 1 cut(s) 162
CfoI GCGC 6 cut(s) 1074, 2534, 2536, 2551, 2701, 2801
Cfr10I RCCGGY 2 cut(s) 197, 2502
Cfr13I GGNCC 7 cut(s) 58, 85, 391, 1481, 1882, 2221, 2312
Cfr42I CCGCGG 1 cut(s) 256
ClaI ATCGAT 1 cut(s) 360
CseI GACGC 2 cut(s) 1119, 2790
Csp6I GTAC 4 cut(s) 47, 556, 1117, 2675
CspAI ACCGGT 1 cut(s) 2502
CspCI CAANNNNNGTGG 2 cut(s) 2757, 2792
CviQI GTAC 4 cut(s) 47, 556, 1117, 2675
DinI GGCGCC 1 cut(s) 2550
DpnI GATC 8 cut(s) 92, 330, 1699, 1808, 1977, 2153, 2201, 2843
DpnII GATC 8 cut(s) 90, 328, 1697, 1806, 1975, 2151, 2199, 2841
EaeI YGGCCR 1 cut(s) 250
EagI CGGCCG 1 cut(s) 250
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
EciI GGCGGA 2 cut(s) 478, 602
Ecl136II GAGCTC 1 cut(s) 13
EclXI CGGCCG 1 cut(s) 250
Eco130I CCWWGG 2 cut(s) 1774, 2743
Eco147I AGGCCT 2 cut(s) 1155, 2644
Eco24I GRGCYC 1 cut(s) 15
Eco31I GGTCTC 1 cut(s) 426
Eco47I GGWCC 4 cut(s) 58, 391, 1882, 2312
Eco47III AGCGCT 1 cut(s) 2700
Eco52I CGGCCG 1 cut(s) 250
Eco53kI GAGCTC 1 cut(s) 13
Eco57I CTGAAG 3 cut(s) 554, 636, 2856
Eco72I CACGTG 1 cut(s) 1897
Eco81I CCTNAGG 1 cut(s) 2457
Eco88I CYCGRG 4 cut(s) 341, 920, 2360, 2702
EcoICRI GAGCTC 1 cut(s) 13
EcoNI CCTNNNNNAGG 1 cut(s) 2874
EcoRII CCWGG 4 cut(s) 54, 685, 2314, 2333
EcoT14I CCWWGG 2 cut(s) 1774, 2743
EcoT38I GRGCYC 1 cut(s) 15
EgeI GGCGCC 1 cut(s) 2550
EheI GGCGCC 1 cut(s) 2550
ErhI CCWWGG 2 cut(s) 1774, 2743
Esp3I CGTCTC 1 cut(s) 2808
FalI AAGNNNNNCTT 2 cut(s) 926, 958
FaqI GGGAC 6 cut(s) 120, 144, 377, 460, 704, 2143
FauI CCCGC 6 cut(s) 97, 811, 1400, 1668, 1937, 2276
FauNDI CATATG 1 cut(s) 1303
FbaI TGATCA 2 cut(s) 2151, 2841
FblI GTMKAC 1 cut(s) 1527
FriOI GRGCYC 1 cut(s) 15
FspBI CTAG 5 cut(s) 578, 948, 1004, 2225, 2498
FspI TGCGCA 1 cut(s) 1073
GlaI GCGC 6 cut(s) 1073, 2533, 2535, 2550, 2700, 2800
GsaI CCCAGC 3 cut(s) 172, 2031, 2789
HaeII RGCGCY 2 cut(s) 2552, 2702
HaeIII GGCC 9 cut(s) 87, 252, 258, 1155, 1482, 2222, 2644, 2712, 2880
HgaI GACGC 2 cut(s) 1119, 2790
HhaI GCGC 6 cut(s) 1074, 2534, 2536, 2551, 2701, 2801
Hin1I GRCGYC 1 cut(s) 2549
Hin6I GCGC 6 cut(s) 1072, 2532, 2534, 2549, 2699, 2799
HinP1I GCGC 6 cut(s) 1072, 2532, 2534, 2549, 2699, 2799
HincII GTYRAC 1 cut(s) 1528
HindII GTYRAC 1 cut(s) 1528
HindIII AAGCTT 3 cut(s) 967, 1040, 1991
HphI GGTGA 9 cut(s) 617, 905, 1298, 1637, 1721, 2021, 2323, 2641, 2701
Hpy166II GTNNAC 5 cut(s) 508, 1528, 1628, 1729, 2089
Hpy8I GTNNAC 5 cut(s) 508, 1528, 1628, 1729, 2089
Hpy99I CGWCG 5 cut(s) 77, 80, 155, 227, 520
HpyAV CCTTC 8 cut(s) 611, 1056, 1731, 2111, 2222, 2702, 2870, 2880
HpyCH4III ACNGT 6 cut(s) 158, 1121, 1882, 2140, 2210, 2572
HpyCH4IV ACGT 6 cut(s) 78, 494, 980, 1896, 1915, 2667
HpySE526I ACGT 6 cut(s) 78, 494, 980, 1896, 1915, 2667
Hsp92I GRCGYC 1 cut(s) 2549
HspAI GCGC 6 cut(s) 1072, 2532, 2534, 2549, 2699, 2799
KasI GGCGCC 1 cut(s) 2548
Ksp22I TGATCA 2 cut(s) 2151, 2841
KspI CCGCGG 1 cut(s) 256
Kzo9I GATC 8 cut(s) 90, 328, 1697, 1806, 1975, 2151, 2199, 2841
LmnI GCTCC 4 cut(s) 40, 345, 1930, 2696
Lsp1109I GCAGC 7 cut(s) 177, 255, 1151, 1856, 1909, 2266, 2529
LweI GCATC 3 cut(s) 555, 2002, 2517
MaeI CTAG 5 cut(s) 578, 948, 1004, 2225, 2498
MaeII ACGT 6 cut(s) 78, 494, 980, 1896, 1915, 2667
MaeIII GTNAC 6 cut(s) 1319, 1705, 1799, 1911, 2629, 2681
MalI GATC 8 cut(s) 92, 330, 1699, 1808, 1977, 2153, 2201, 2843
MboI GATC 8 cut(s) 90, 328, 1697, 1806, 1975, 2151, 2199, 2841
MfeI CAATTG 1 cut(s) 1324
MflI RGATCY 2 cut(s) 328, 1697
MhlI GDGCHC 4 cut(s) 15, 246, 1439, 2035
MluI ACGCGT 1 cut(s) 1182
Mly113I GGCGCC 1 cut(s) 2549
MlyI GAGTC 6 cut(s) 186, 213, 279, 482, 2236, 2296
MmeI TCCRAC 8 cut(s) 138, 154, 321, 846, 1230, 1247, 1788, 1814
MroXI GAANNNNTTC 1 cut(s) 1544
MseI TTAA 8 cut(s) 651, 1059, 1292, 2184, 2295, 2513, 2574, 2792
MslI CAYNNNNRTG 1 cut(s) 1690
MspA1I CMGCKG 3 cut(s) 168, 255, 1847
MspCI CTTAAG 1 cut(s) 2512
MunI CAATTG 1 cut(s) 1324
Mva1269I GAATGC 2 cut(s) 1341, 2729
MvaI CCWGG 4 cut(s) 56, 687, 2316, 2335
MvnI CGCG 4 cut(s) 255, 1184, 2534, 2801
NarI GGCGCC 1 cut(s) 2549
NciI CCSGG 7 cut(s) 119, 241, 326, 395, 532, 2714, 2825
NcoI CCATGG 1 cut(s) 1774
NdeI CATATG 1 cut(s) 1303
NdeII GATC 8 cut(s) 90, 328, 1697, 1806, 1975, 2151, 2199, 2841
NheI GCTAGC 1 cut(s) 1003
NlaIV GGNNCC 6 cut(s) 60, 393, 1253, 2207, 2348, 2550
NmeAIII GCCGAG 1 cut(s) 235
NmuCI GTSAC 5 cut(s) 1319, 1799, 1911, 2629, 2681
NsbI TGCGCA 1 cut(s) 1073
NspI RCATGY 1 cut(s) 2565
PaeR7I CTCGAG 2 cut(s) 920, 2702
PauI GCGCGC 1 cut(s) 2532
PceI AGGCCT 2 cut(s) 1155, 2644
PcsI WCGNNNNNNNCGW 3 cut(s) 150, 927, 2110
PctI GAATGC 2 cut(s) 1341, 2729
PdmI GAANNNNTTC 1 cut(s) 1544
PflFI GACNNNGTC 1 cut(s) 2245
PflMI CCANNNNNTGG 3 cut(s) 496, 1261, 1796
PfoI TCCNGGA 2 cut(s) 393, 530
PinAI ACCGGT 1 cut(s) 2502
PleI GAGTC 6 cut(s) 185, 212, 279, 481, 2236, 2295
PluTI GGCGCC 1 cut(s) 2552
PmaCI CACGTG 1 cut(s) 1897
PmlI CACGTG 1 cut(s) 1897
PpsI GAGTC 6 cut(s) 185, 212, 279, 481, 2236, 2295
Ppu21I YACGTR 1 cut(s) 1897
PshAI GACNNNNGTC 1 cut(s) 156
Psp124BI GAGCTC 1 cut(s) 15
Psp6I CCWGG 4 cut(s) 54, 685, 2314, 2333
PspCI CACGTG 1 cut(s) 1897
PspFI CCCAGC 3 cut(s) 168, 2027, 2785
PspGI CCWGG 4 cut(s) 54, 685, 2314, 2333
PspN4I GGNNCC 6 cut(s) 60, 393, 1253, 2207, 2348, 2550
PspPI GGNCC 7 cut(s) 58, 85, 391, 1481, 1882, 2221, 2312
PspXI VCTCGAGB 1 cut(s) 2702
PstI CTGCAG 4 cut(s) 167, 273, 1161, 1392
PstNI CAGNNNCTG 1 cut(s) 162
PsuI RGATCY 2 cut(s) 328, 1697
PsyI GACNNNGTC 1 cut(s) 2245
PteI GCGCGC 1 cut(s) 2532
PvuII CAGCTG 2 cut(s) 168, 1847
RsaI GTAC 4 cut(s) 48, 557, 1118, 2676
RsaNI GTAC 4 cut(s) 47, 556, 1117, 2675
RseI CAYNNNNRTG 1 cut(s) 1690
SacI GAGCTC 1 cut(s) 15
SacII CCGCGG 1 cut(s) 256
SalI GTCGAC 1 cut(s) 1526
SaqAI TTAA 8 cut(s) 651, 1059, 1292, 2184, 2295, 2513, 2574, 2792
Sau3AI GATC 8 cut(s) 90, 328, 1697, 1806, 1975, 2151, 2199, 2841
Sau96I GGNCC 7 cut(s) 58, 85, 391, 1481, 1882, 2221, 2312
SbfI CCTGCAGG 1 cut(s) 1161
ScaI AGTACT 1 cut(s) 2676
SchI GAGTC 6 cut(s) 186, 213, 279, 482, 2236, 2296
SdaI CCTGCAGG 1 cut(s) 1161
SduI GDGCHC 4 cut(s) 15, 246, 1439, 2035
SfaNI GCATC 3 cut(s) 555, 2002, 2517
SfcI CTRYAG 5 cut(s) 163, 269, 1157, 1388, 1491
SfoI GGCGCC 1 cut(s) 2550
Sfr274I CTCGAG 2 cut(s) 920, 2702
Sfr303I CCGCGG 1 cut(s) 256
SgrBI CCGCGG 1 cut(s) 256
SinI GGWCC 4 cut(s) 58, 391, 1882, 2312
SlaI CTCGAG 2 cut(s) 920, 2702
SmiMI CAYNNNNRTG 1 cut(s) 1690
Sse8387I CCTGCAGG 1 cut(s) 1161
SseBI AGGCCT 2 cut(s) 1155, 2644
SspDI GGCGCC 1 cut(s) 2548
SspI AATATT 1 cut(s) 2654
SspMI CTAG 5 cut(s) 578, 948, 1004, 2225, 2498
SstI GAGCTC 1 cut(s) 15
StuI AGGCCT 2 cut(s) 1155, 2644
StyI CCWWGG 2 cut(s) 1774, 2743
TaaI ACNGT 6 cut(s) 158, 1121, 1882, 2140, 2210, 2572
TaiI ACGT 6 cut(s) 81, 497, 983, 1899, 1918, 2670
TaqII GACCGA 2 cut(s) 379, 1055
TatI WGTACW 2 cut(s) 1116, 2674
TauI GCSGC 5 cut(s) 255, 258, 268, 1301, 2341
Tru1I TTAA 8 cut(s) 651, 1059, 1292, 2184, 2295, 2513, 2574, 2792
Tru9I TTAA 8 cut(s) 651, 1059, 1292, 2184, 2295, 2513, 2574, 2792
TscAI CASTG 3 cut(s) 316, 463, 1081
TseFI GTSAC 5 cut(s) 1319, 1799, 1911, 2629, 2681
TseI GCWGC 7 cut(s) 165, 268, 1139, 1844, 1922, 2254, 2517
Tsp45I GTSAC 5 cut(s) 1319, 1799, 1911, 2629, 2681
TspGWI ACGGA 4 cut(s) 240, 1714, 2235, 2325
TspRI CASTG 3 cut(s) 316, 463, 1081
Tth111I GACNNNGTC 1 cut(s) 2245
Van91I CCANNNNNTGG 3 cut(s) 496, 1261, 1796
Vha464I CTTAAG 1 cut(s) 2512
VpaK11BI GGWCC 4 cut(s) 58, 391, 1882, 2312
XagI CCTNNNNNAGG 1 cut(s) 2874
XapI RAATTY 5 cut(s) 628, 736, 808, 897, 1544
XceI RCATGY 1 cut(s) 2565
XcmI CCANNNNNNNNNTGG 1 cut(s) 2534
XhoI CTCGAG 2 cut(s) 920, 2702
XmiI GTMKAC 1 cut(s) 1527
XmnI GAANNNNTTC 1 cut(s) 1544
XspI CTAG 5 cut(s) 578, 948, 1004, 2225, 2498
ZrmI AGTACT 1 cut(s) 2676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.